FastQCFastQC Report
Thu 26 May 2016
SRR938802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811158
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60940.7512716388175916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49100.6053074740062971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33740.41594855749434756No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT27700.3414871085534507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17820.2196859304845665No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT13520.16667529630478894RNA PCR Primer, Index 26 (100% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.13893717376885883No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.10195301038761868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36300.039.5960121
GTATCAA57800.036.1482961
ATCAACG73200.028.472913
TATCAAC73450.028.4406362
TCAACGC73700.028.2797434
CAACGCA75450.027.6238175
AACGCAG77000.026.992786
GTACATG44600.025.611061
CATGGGA29300.024.9535034
TACATGG46600.024.1457582
ACATGGG46350.023.8662743
ACGCAGA86500.023.8073487
ATGGGAG9800.023.7382355
GTAGTAC1000.001658001323.7382353
GACCGCT1000.001663365823.7250657
CGCAGAG86950.023.6295668
TAAGGTG2503.8380676E-1022.7887085
TAAGGCG2303.6434358E-922.7061395
GAGTACT52050.022.70088612-13
GCAGAGT90750.022.3786859
Produced by FastQC (version 0.11.5)