##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938802_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 811158 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97610206642849 31.0 31.0 34.0 30.0 34.0 2 32.168859827555174 33.0 31.0 34.0 30.0 34.0 3 32.22841419304254 34.0 31.0 34.0 30.0 34.0 4 35.89485648911803 37.0 35.0 37.0 35.0 37.0 5 35.80009320009172 37.0 35.0 37.0 35.0 37.0 6 35.83315087812732 37.0 35.0 37.0 35.0 37.0 7 35.74056842193506 37.0 35.0 37.0 33.0 37.0 8 35.73506024720215 37.0 35.0 37.0 33.0 37.0 9 37.3480271414447 39.0 37.0 39.0 34.0 39.0 10-11 37.28784466158258 39.0 37.0 39.0 33.5 39.0 12-13 37.20891552570522 39.0 37.0 39.0 33.0 39.0 14-15 38.38394554451784 40.0 38.0 41.0 33.0 41.0 16-17 38.32920220228365 40.0 38.0 41.0 33.0 41.0 18-19 38.470631492261674 40.0 38.0 41.0 33.5 41.0 20-21 38.470535949839615 40.0 38.0 41.0 34.0 41.0 22-23 38.36705241149073 40.0 38.0 41.0 34.0 41.0 24-25 38.26606407136464 40.0 38.0 41.0 33.0 41.0 26-27 38.06286937933177 40.0 37.0 41.0 33.0 41.0 28-29 37.857458103106914 40.0 37.0 41.0 33.0 41.0 30-31 37.557266894982234 40.0 36.5 41.0 32.0 41.0 32-33 37.295462166433666 39.5 36.0 41.0 32.0 41.0 34-35 37.162965784717656 40.0 36.0 41.0 31.0 41.0 36-37 36.98645023534256 40.0 35.0 41.0 31.0 41.0 38-39 36.786523710547144 39.0 35.0 41.0 31.0 41.0 40-41 36.55882639387148 39.0 35.0 41.0 30.0 41.0 42-43 36.26675505881715 39.0 35.0 41.0 30.0 41.0 44-45 35.90623589978771 39.0 35.0 41.0 29.5 41.0 46-47 35.429902065935366 39.0 35.0 41.0 26.5 41.0 48-49 34.994252661010556 39.0 35.0 41.0 24.5 41.0 50-51 34.6989513756876 38.5 34.0 40.0 24.0 41.0 52-53 34.445736219084324 38.0 34.0 40.0 23.0 41.0 54-55 34.133911642368076 38.0 34.0 40.0 22.5 41.0 56-57 33.830046674014184 38.0 33.5 40.0 21.0 41.0 58-59 33.47674620727405 37.5 33.0 40.0 19.5 41.0 60-61 33.06842994829614 37.0 33.0 40.0 16.5 41.0 62-63 32.682314666193264 36.5 32.0 39.5 12.5 41.0 64-65 32.24490111667517 36.0 32.0 39.0 8.0 41.0 66-67 31.800080872037263 35.0 31.0 39.0 4.5 40.0 68-69 31.45975753182487 35.0 31.0 38.5 2.0 40.0 70-71 31.0396525707692 35.0 31.0 38.0 2.0 40.0 72-73 30.57624716762949 35.0 30.5 37.0 2.0 39.5 74-75 30.092898054386445 35.0 30.0 36.5 2.0 39.0 76-77 28.16507758044672 32.5 27.0 35.0 2.0 37.0 78-79 29.0727398361355 34.0 29.0 35.0 2.0 37.5 80-81 29.103377517080517 34.0 29.0 35.0 2.0 37.0 82-83 28.7818045312997 34.0 29.0 35.0 2.0 37.0 84-85 28.403437431425196 34.0 29.0 35.0 2.0 36.0 86-87 27.85644103861393 34.0 27.5 35.0 2.0 36.0 88-89 27.27755874934353 33.5 26.0 35.0 2.0 35.5 90-91 26.77414079624438 33.0 25.0 35.0 2.0 35.0 92-93 26.491788899326643 33.0 25.0 35.0 2.0 35.0 94-95 26.169925587863275 33.0 24.5 35.0 2.0 35.0 96-97 25.60332265723817 33.0 21.5 35.0 2.0 35.0 98-99 24.84206036308586 32.0 10.0 34.0 2.0 35.0 100-101 21.626367489441023 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1536312706366587 1101 2 0.21666234363360815 1101 3 0.04860742734540935 1101 4 0.17666935222792546 1101 5 -0.15594492660613923 1101 6 0.12280456822745123 1101 7 0.22442567158881133 1101 8 0.27819697575180413 1101 9 0.18610522526005724 1101 10-11 0.21594574550974954 1101 12-13 0.09371193492538055 1101 14-15 0.19122119476528354 1101 16-17 0.2921808037120428 1101 18-19 0.28179279496706755 1101 20-21 0.3467941433224979 1101 22-23 0.27869423765493906 1101 24-25 0.4965583359096044 1101 26-27 0.2812840215527217 1101 28-29 0.2966395599212248 1101 30-31 0.5007130799987678 1101 32-33 0.31244823321255666 1101 34-35 0.42778943826792926 1101 36-37 0.5622843904294896 1101 38-39 0.49183725596078176 1101 40-41 0.29201269379899486 1101 42-43 0.3391669876154353 1101 44-45 0.27298118105085933 1101 46-47 0.3949492095638263 1101 48-49 0.17466531288419418 1101 50-51 0.1842604990727068 1101 52-53 0.3896501581805154 1101 54-55 0.40528373237426507 1101 56-57 0.401869589650822 1101 58-59 0.41163212052744313 1101 60-61 0.4726216997144874 1101 62-63 0.30330141038287906 1101 64-65 0.3713808879996563 1101 66-67 0.5074487371559577 1101 68-69 0.1616678993724463 1101 70-71 0.5299371363453211 1101 72-73 0.2811497221794248 1101 74-75 -0.24237411379994356 1101 76-77 -0.3399098372930247 1101 78-79 -0.18769443298181088 1101 80-81 0.12488192483521132 1101 82-83 0.25448809321013854 1101 84-85 0.3162194605206672 1101 86-87 0.2920562449280837 1101 88-89 0.27633175553344813 1101 90-91 0.1874246529489092 1101 92-93 0.13496820096526818 1101 94-95 0.24495703238909883 1101 96-97 0.06312971805422407 1101 98-99 -0.049989467723541736 1101 100-101 0.27969069850108497 1102 1 0.3407609325397587 1102 2 0.26397848166079285 1102 3 0.2610856427514747 1102 4 0.20242745609686352 1102 5 2.418980714509189E-4 1102 6 0.1650666804616776 1102 7 0.2547846816968615 1102 8 0.27262883354197953 1102 9 0.3614014957375815 1102 10-11 0.5407366134079652 1102 12-13 0.46524382757090166 1102 14-15 0.37542300724349786 1102 16-17 0.6207055510107509 1102 18-19 0.5305510731127541 1102 20-21 0.6142653876584987 1102 22-23 0.5282193335071597 1102 24-25 0.5690226091755477 1102 26-27 0.44996648931152805 1102 28-29 0.46331784503799156 1102 30-31 0.48501510160908623 1102 32-33 0.6438136915604176 1102 34-35 0.7355142552773444 1102 36-37 0.7282089285961035 1102 38-39 0.8759650008196189 1102 40-41 0.961880243467867 1102 42-43 0.9100322716865392 1102 44-45 0.8565730248988928 1102 46-47 0.7177663932515159 1102 48-49 0.6465893282205499 1102 50-51 0.49952714250246544 1102 52-53 0.16431903235270084 1102 54-55 0.207632111600482 1102 56-57 0.29443232928135643 1102 58-59 0.41428112714995535 1102 60-61 0.5367947565287068 1102 62-63 0.6487179318189149 1102 64-65 0.7586630420603058 1102 66-67 1.03083667720475 1102 68-69 0.7239413395955197 1102 70-71 0.5234409704338567 1102 72-73 0.5687325036363724 1102 74-75 0.7333839900689973 1102 76-77 0.6933813791586978 1102 78-79 0.3570293383670915 1102 80-81 0.36542305414855747 1102 82-83 0.5674063574136952 1102 84-85 0.7384441032533253 1102 86-87 0.9563120831992578 1102 88-89 0.6511966910510516 1102 90-91 0.839938595089027 1102 92-93 0.7778315679921057 1102 94-95 0.9648768650834221 1102 96-97 1.1404824541992191 1102 98-99 1.578461211955787 1102 100-101 2.0343569585220003 1103 1 0.18945183919571207 1103 2 0.2158400056999028 1103 3 0.09262441334207239 1103 4 -0.08239911414553092 1103 5 0.20989771700810422 1103 6 0.1809967728682622 1103 7 0.2517178543230827 1103 8 0.11479101380178491 1103 9 0.2774487704128177 1103 10-11 0.2242340019174094 1103 12-13 0.2474756475789306 1103 14-15 0.23557099170847806 1103 16-17 0.1616574058386675 1103 18-19 0.06493402476670695 1103 20-21 0.2982248091128952 1103 22-23 0.4379337435781352 1103 24-25 0.2874637501960393 1103 26-27 0.40511581300243193 1103 28-29 0.33402897400544873 1103 30-31 0.29757201871100136 1103 32-33 0.3740182583208309 1103 34-35 0.1868216745889768 1103 36-37 0.0935012146547578 1103 38-39 0.08510689180662467 1103 40-41 0.09572248265362049 1103 42-43 0.3436418203674023 1103 44-45 0.2691450082924902 1103 46-47 0.15730871279082947 1103 48-49 0.11843879922630407 1103 50-51 0.132398442890846 1103 52-53 0.22570580080054015 1103 54-55 -0.06842420284976924 1103 56-57 -0.10594767607555156 1103 58-59 -0.22744915274826383 1103 60-61 -0.4273993642694691 1103 62-63 -0.42239496492675954 1103 64-65 -0.23628640216093544 1103 66-67 -0.2701543499815351 1103 68-69 -0.3188251663640358 1103 70-71 -0.25213657074734286 1103 72-73 -0.04265426481146406 1103 74-75 -0.3377021221581309 1103 76-77 -0.1834617305239057 1103 78-79 -0.5629636306967924 1103 80-81 -0.407395681622166 1103 82-83 -0.6190858750759247 1103 84-85 -0.7256093988224741 1103 86-87 -0.6502166431968845 1103 88-89 -0.6316026259437244 1103 90-91 -0.47791161249098835 1103 92-93 -0.6005633697338872 1103 94-95 -0.6762105863696384 1103 96-97 -0.5366335227660919 1103 98-99 -0.4253589300021581 1103 100-101 0.35651947358828906 1104 1 0.3264697664151157 1104 2 0.22107345743566498 1104 3 0.12108431534721831 1104 4 0.18143455770966455 1104 5 0.00790765767853685 1104 6 0.06089531257158143 1104 7 0.2642158889710373 1104 8 0.18392006603683342 1104 9 0.18094918273565241 1104 10-11 0.2944981452324882 1104 12-13 0.10893036105467502 1104 14-15 0.24878698149185396 1104 16-17 0.3914985091311465 1104 18-19 0.2944382105654739 1104 20-21 0.2741761197219432 1104 22-23 0.3095352889063747 1104 24-25 0.27544060877732335 1104 26-27 0.24762693930156843 1104 28-29 0.2553202343656622 1104 30-31 0.20339716174050437 1104 32-33 0.31240647711825886 1104 34-35 0.4368922668257582 1104 36-37 0.6290941413126347 1104 38-39 0.5487374505441878 1104 40-41 0.7391185789029251 1104 42-43 0.9591138738634868 1104 44-45 1.0575038671368944 1104 46-47 0.9446239295892127 1104 48-49 1.108628884867521 1104 50-51 0.8445128729066695 1104 52-53 0.9251954510160019 1104 54-55 0.8256673873687177 1104 56-57 0.8239911332901144 1104 58-59 1.0924652381209512 1104 60-61 1.2655139773424011 1104 62-63 0.9749607140582484 1104 64-65 0.8637411929861685 1104 66-67 0.7330335312566554 1104 68-69 0.7062976480842735 1104 70-71 0.8971226920771791 1104 72-73 1.0015509147334747 1104 74-75 0.9119497297650234 1104 76-77 0.8008090356264752 1104 78-79 0.9084272609794226 1104 80-81 0.8801978932007906 1104 82-83 1.0207124236511653 1104 84-85 1.1252604900589276 1104 86-87 1.4306964994732354 1104 88-89 1.4001903526957022 1104 90-91 1.3748402012481833 1104 92-93 1.649191161806403 1104 94-95 1.4698712236153106 1104 96-97 1.393002863039733 1104 98-99 1.5055352811135485 1104 100-101 1.989214428489479 1105 1 -0.13368607806462407 1105 2 -0.020703712080667458 1105 3 -0.017373793849913 1105 4 0.08324848270541452 1105 5 -0.12457106139768115 1105 6 -0.051129403072252444 1105 7 -0.10711109606645408 1105 8 0.017995107694602552 1105 9 0.12557591509871457 1105 10-11 0.08032577481581171 1105 12-13 0.04777098910886224 1105 14-15 -0.06276091052372834 1105 16-17 0.017311985205012093 1105 18-19 0.03569631415107466 1105 20-21 -0.12489439374804334 1105 22-23 -0.193862020991574 1105 24-25 0.03745667332231761 1105 26-27 -0.021502510753535375 1105 28-29 -0.20942303063697665 1105 30-31 -0.1150458898683695 1105 32-33 -0.10183151694911174 1105 34-35 -0.07937335776019694 1105 36-37 -0.2848001469357442 1105 38-39 -0.18601128569394376 1105 40-41 -0.3934349604876317 1105 42-43 -0.22256076064707742 1105 44-45 -0.3376222128865507 1105 46-47 -0.2248487586579202 1105 48-49 -0.07323046229405605 1105 50-51 -0.2693753361911746 1105 52-53 -0.052769966411204905 1105 54-55 -0.2150184966878541 1105 56-57 -0.1679017738999633 1105 58-59 -0.20945187655832598 1105 60-61 -0.37406253967843384 1105 62-63 -0.4745987034032524 1105 64-65 -0.46259696054200106 1105 66-67 -0.3499453148582212 1105 68-69 -0.4913105958673647 1105 70-71 -0.36712161495421114 1105 72-73 -0.36831941230923704 1105 74-75 -0.3687986916042796 1105 76-77 -0.2148736140102585 1105 78-79 -0.3579135736100092 1105 80-81 -0.4472298311549352 1105 82-83 -0.2559777750435295 1105 84-85 -0.2004432495539099 1105 86-87 -0.04068550967043194 1105 88-89 -0.13603982576633555 1105 90-91 -0.003014856215376227 1105 92-93 0.11200468620172188 1105 94-95 0.3307444078587771 1105 96-97 0.33360098910261016 1105 98-99 0.19243720571025946 1105 100-101 0.9476766839790294 1106 1 0.0730419556102575 1106 2 0.09198760703834097 1106 3 0.1409005646716608 1106 4 0.14806719647344835 1106 5 0.1710214109562216 1106 6 0.07338555457002371 1106 7 -0.04916108014363374 1106 8 0.062120190138593046 1106 9 0.09511880182330401 1106 10-11 0.07732005920194496 1106 12-13 0.17559502607999633 1106 14-15 0.14667324823057726 1106 16-17 0.24088500605863317 1106 18-19 0.07730015736184015 1106 20-21 -0.009786729814670991 1106 22-23 0.10711930780186663 1106 24-25 0.030419691625262146 1106 26-27 0.041898241769594335 1106 28-29 0.19034695627762943 1106 30-31 0.09716004730666583 1106 32-33 0.10246432455315357 1106 34-35 0.06142438316116028 1106 36-37 0.11773482667462787 1106 38-39 0.23358902883838795 1106 40-41 0.2085835401711691 1106 42-43 0.052570508303723784 1106 44-45 0.07588894289902015 1106 46-47 0.02836259529508567 1106 48-49 -0.07957544268106886 1106 50-51 -0.07738308177617625 1106 52-53 -0.17008267704900248 1106 54-55 -0.12311061058961315 1106 56-57 -0.17230583021996182 1106 58-59 -0.019906907038077293 1106 60-61 -0.013244604694868656 1106 62-63 0.3279801900285477 1106 64-65 0.10762117457106513 1106 66-67 0.07206153421908468 1106 68-69 0.18657147262038976 1106 70-71 0.27341124869360556 1106 72-73 0.24171785162172554 1106 74-75 0.5315878330096844 1106 76-77 0.6848239278380888 1106 78-79 0.38528828702077433 1106 80-81 0.37492356825433504 1106 82-83 0.48973702382330586 1106 84-85 0.6541942157139644 1106 86-87 0.6601646633676275 1106 88-89 0.8076945061014946 1106 90-91 0.31899440769747045 1106 92-93 0.45374089258564254 1106 94-95 0.27081799997192135 1106 96-97 0.3670215583699026 1106 98-99 0.4290925981134919 1106 100-101 1.0037363364540077 1107 1 0.036364270152084543 1107 2 -0.1376261396485745 1107 3 -0.3022648065424889 1107 4 -0.07395251822919136 1107 5 -0.11798660000300742 1107 6 -0.04359827460892518 1107 7 -0.0988096700361254 1107 8 -0.12425820111784702 1107 9 -0.31032507679130816 1107 10-11 -0.24815940713119033 1107 12-13 -0.1053916024676127 1107 14-15 -0.07168867439963122 1107 16-17 -0.18913077761568786 1107 18-19 -0.25938795127748193 1107 20-21 -0.17379622979977682 1107 22-23 -0.3386131440281446 1107 24-25 -0.2827745666909536 1107 26-27 -0.1960540498412371 1107 28-29 -0.10606598037920634 1107 30-31 -0.15672109864765105 1107 32-33 -0.20475819547424123 1107 34-35 -0.0590139860576997 1107 36-37 -0.23547849117283448 1107 38-39 -0.15855040354495742 1107 40-41 -0.34238804921505306 1107 42-43 -0.24784641894246562 1107 44-45 -0.006424407707015689 1107 46-47 -0.16488765039417785 1107 48-49 -0.023372160868028402 1107 50-51 -0.2982584800905954 1107 52-53 -0.2259248700863239 1107 54-55 -0.09751872280260443 1107 56-57 -0.1481061816300695 1107 58-59 -0.09242618992321638 1107 60-61 -0.24441512793855935 1107 62-63 -0.10924098987556619 1107 64-65 -0.006243889903750244 1107 66-67 -0.03026730225609242 1107 68-69 -0.12215943062270895 1107 70-71 -0.2038215456123602 1107 72-73 -0.11846261831484384 1107 74-75 -0.021044764744864608 1107 76-77 -0.42476688785028927 1107 78-79 -0.3530958220950424 1107 80-81 -0.1073786146576019 1107 82-83 -0.13437477223970618 1107 84-85 -0.04222020996875386 1107 86-87 0.12235829629296546 1107 88-89 0.10214341762357648 1107 90-91 0.1860072728810671 1107 92-93 0.45568779392278813 1107 94-95 0.637405748266989 1107 96-97 0.47623341697200416 1107 98-99 0.7159772581688806 1107 100-101 1.400547587533552 1108 1 0.15237863959810483 1108 2 0.11233289482549225 1108 3 0.25046412772359616 1108 4 0.1745884788086869 1108 5 0.20512425901256393 1108 6 0.16731746245542212 1108 7 0.22090735271549278 1108 8 0.14398406270014164 1108 9 0.28230112854051725 1108 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-1.09656764130942 2316 54-55 -0.7862411142237917 2316 56-57 -0.8537776171482037 2316 58-59 -0.5350368161868744 2316 60-61 -0.6454766026141598 2316 62-63 -0.7295888003840929 2316 64-65 -0.8566949686537839 2316 66-67 -0.8480775548601933 2316 68-69 -0.8494743283781929 2316 70-71 -0.7809848041284688 2316 72-73 -0.8297722182943694 2316 74-75 -0.6822315177456595 2316 76-77 -0.7882493437661395 2316 78-79 -0.615914136532119 2316 80-81 -0.8825411309732196 2316 82-83 -0.7179273533059174 2316 84-85 -1.0483420844089046 2316 86-87 -1.1416859286193741 2316 88-89 -1.6843869230208561 2316 90-91 -2.0405295940091115 2316 92-93 -2.118740199832491 2316 94-95 -2.026493875514138 2316 96-97 -2.0273131497108707 2316 98-99 -1.7793161669584414 2316 100-101 -1.699180485560401 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 17.0 5 35.0 6 123.0 7 281.0 8 527.0 9 971.0 10 1758.0 11 2585.0 12 3741.0 13 4995.0 14 5537.0 15 7170.0 16 9187.0 17 12022.0 18 10998.0 19 7162.0 20 6647.0 21 6787.0 22 7172.0 23 7628.0 24 8679.0 25 9847.0 26 11005.0 27 12545.0 28 14999.0 29 17850.0 30 21494.0 31 25981.0 32 31409.0 33 39191.0 34 51494.0 35 71517.0 36 102879.0 37 154646.0 38 130419.0 39 11853.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.00297032883665 20.82961630369512 15.023389486222072 22.144023881246156 2 15.855727244260676 22.565764006519075 38.75040374378358 22.828105005436672 3 17.922402294004375 27.086091735518853 32.173263408608435 22.81824256186834 4 12.632434125040993 18.69524802812769 37.25501073773543 31.417307109095887 5 12.409458489630731 38.58443265585964 34.42513385469459 14.580974999815034 6 29.10906127561575 37.69630215923129 17.924677175566682 15.269959389586276 7 25.586137349320353 35.21064946656509 21.882173386689153 17.321039797425406 8 25.47234940665074 34.46813074641438 21.11598480197446 18.943535044960413 9 26.170487130743947 16.447473858360517 21.411981389568986 35.970057621326546 10-11 23.705246080295083 27.412415337085992 28.218485177980124 20.6638534046388 12-13 25.184242773910874 25.56499473592074 28.53506961652354 20.71569287364484 14-15 22.20473693164587 26.5805182220973 27.67863967315862 23.536105173098214 16-17 20.440294992960506 29.915008296985224 28.181209609215696 21.463487100838574 18-19 20.2671366121027 27.645723767749313 31.673853922417088 20.4132856977309 20-21 21.319397700571283 26.811237761323937 31.475742087238245 20.393622450866538 22-23 21.127449892622646 27.305469464641906 31.26209690343928 20.30498373929617 24-25 20.90453847234927 27.378956400387594 31.63770381456542 20.07880131269771 26-27 20.549419525684 27.41136933256241 31.671139756419 20.368071385334595 28-29 20.124254460906506 27.54611308770918 31.900073721765676 20.429558729618645 30-31 20.59888455763242 27.167025413051466 31.473892879069183 20.76019715024693 32-33 20.484172010878275 27.484349534862506 31.469146578101924 20.562331876157298 34-35 20.89277964630574 27.63507648909623 31.18027952301577 20.291864341582254 36-37 20.945448985618576 27.30516151743841 30.87909357635496 20.870295920588056 38-39 20.64397407088893 27.19591397650598 31.122392678694172 21.037719273910913 40-41 20.720615757898553 27.167062426488535 31.2606491447396 20.851672670873313 42-43 20.80149871839604 27.21802555317331 30.861134296560323 21.11934143187033 44-45 20.384045901566587 27.50709814862531 30.734767798384222 21.374088151423884 46-47 20.92756624812767 27.46786988923202 29.993589387971475 21.610974474668836 48-49 20.923398975151777 27.61363727424645 29.637197811240785 21.825765939360988 50-51 20.605233505679536 27.86658086340762 29.550038340249372 21.978147290663472 52-53 20.78466833835085 28.00403867064123 29.534320070812342 21.676972920195574 54-55 20.40194388762732 27.816590602570646 29.385335532658246 22.396129977143787 56-57 20.680795928267585 28.148124861693873 28.881463249557576 22.28961596048097 58-59 20.544321930676713 27.81998777053982 29.28185207284419 22.353838225939278 60-61 20.548869830651817 28.143740996462384 29.099187007906778 22.20820216497902 62-63 20.244738498295327 28.178644803097818 29.1546646958059 22.421952002800957 64-65 19.999371259533586 28.11493129161168 28.983702677263203 22.901994771591532 66-67 19.981593046042658 28.110735150069686 28.690313787400296 23.217358016487356 68-69 20.05130945754611 27.769302224663186 28.821626899371456 23.35776141841926 70-71 20.02710939175845 27.7644429322031 28.59467575983964 23.613771916198818 72-73 20.14256162178022 27.597767635898308 28.378441684603985 23.881229057717483 74-75 19.8602854698368 27.686789438257097 28.18842044790508 24.26450464400102 76-77 19.77079095745534 27.420343628538756 28.547084560152264 24.26178085385364 78-79 19.846738801686307 27.349192338791813 28.361284110338186 24.442784749183698 80-81 19.837908941513827 27.367721589991483 28.126829965887897 24.667539502606797 82-83 19.715206980702103 27.125977238502596 28.193781548553567 24.965034232241738 84-85 19.52048799370776 26.80371764810308 28.17348777919961 25.502306578989543 86-87 19.622117148496816 26.596493652898417 28.093413736740754 25.687975461864017 88-89 19.783403684661103 26.347046481361435 28.034921640332342 25.83462819364512 90-91 19.55050339412016 26.27859631318725 28.068971491865867 26.10192880082672 92-93 19.54012431671421 26.14947346619754 28.051948212053446 26.258454005034803 94-95 19.281937926821676 25.62998093460502 28.273740361743215 26.814340776830093 96-97 19.390242098333495 25.337418850581518 28.22341639976429 27.048922651320705 98-99 19.26967372571065 25.44781657827452 28.14205124032556 27.140458455689277 100-101 19.26897479314823 24.928727600433213 28.573313024680434 27.228984581738114 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1130.0 1 808.5 2 590.5 3 705.5 4 629.0 5 626.5 6 935.5 7 1538.0 8 2172.0 9 2410.5 10 2055.5 11 1763.5 12 1769.0 13 1761.0 14 1842.0 15 1908.0 16 2022.5 17 2159.5 18 2284.0 19 2395.5 20 2575.5 21 2916.5 22 3294.0 23 3723.0 24 4245.5 25 4939.5 26 6039.5 27 7289.0 28 8269.5 29 9249.0 30 10700.5 31 12446.5 32 14365.0 33 15829.5 34 17371.0 35 18902.5 36 20163.0 37 21568.0 38 22133.0 39 22124.5 40 22675.0 41 24124.0 42 25286.0 43 26314.0 44 27446.5 45 28757.5 46 29483.5 47 29794.5 48 30831.5 49 30554.5 50 28876.5 51 27283.5 52 25958.0 53 24919.5 54 23723.0 55 21765.0 56 19466.5 57 17227.5 58 15561.0 59 14103.0 60 12134.5 61 10175.5 62 8577.5 63 6770.5 64 4984.5 65 3847.0 66 2860.0 67 2214.0 68 1638.0 69 1052.5 70 800.0 71 654.0 72 530.0 73 423.5 74 354.5 75 292.0 76 204.5 77 124.5 78 93.5 79 70.5 80 42.5 81 25.5 82 17.0 83 11.0 84 5.0 85 4.5 86 5.0 87 4.0 88 1.5 89 0.5 90 0.5 91 1.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2243705911795236 2 0.0 3 0.0 4 0.0 5 0.025149231099243306 6 0.004438099605748818 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002465610892082677 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.931221784165354E-4 26-27 0.0013560859906454723 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007643393765456298 36-37 0.01806059978450561 38-39 0.01238969473271545 40-41 0.0073968326762480305 42-43 0.0080132353992687 44-45 0.003821696882728149 46-47 3.6984163381240153E-4 48-49 0.0016642873521558069 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.547624507186023E-4 58-59 2.465610892082677E-4 60-61 0.002280690075176476 62-63 8.013235399268699E-4 64-65 0.001541006807551673 66-67 0.0063489480471128935 68-69 1.8492081690620076E-4 70-71 0.0 72-73 0.0 74-75 4.931221784165354E-4 76-77 0.05633920888408917 78-79 0.13597844069835963 80-81 0.0011095249014372046 82-83 9.246040845310038E-4 84-85 0.0 86-87 0.10201465065992074 88-89 0.3493770634081153 90-91 0.3874707516907927 92-93 4.931221784165354E-4 94-95 3.082013615103346E-4 96-97 0.0 98-99 0.0 100-101 0.0011711651737392715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 811158.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.34550030545856 #Duplication Level Percentage of deduplicated Percentage of total 1 82.04540899971961 49.51071253854052 2 10.672404715514954 12.880632040401698 3 3.0102044761760403 5.449568854097218 4 1.253573440816789 3.0259006582289705 5 0.6944542559010289 2.0953594755801266 6 0.43441964494470603 1.5729162490024775 7 0.29743619502677265 1.2564255198489778 8 0.22550638748357854 1.0886636619818515 9 0.1752364054215038 0.9517255701201734 >10 1.0706070010507263 12.17888409234705 >50 0.08499460848037926 3.518428579071353 >100 0.032689252090025475 3.134909561620067 >500 0.0016344625994327638 0.6963451908509128 >1k 0.0012258469495745729 1.8881952191716944 >5k 2.0430782492909547E-4 0.7513327891369446 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6094 0.7512716388175916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4910 0.6053074740062971 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3374 0.41594855749434756 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 2770 0.3414871085534507 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1782 0.2196859304845665 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 1352 0.16667529630478894 RNA PCR Primer, Index 26 (100% over 22bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1127 0.13893717376885883 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 827 0.10195301038761868 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.2328054460413384E-4 0.0 6 0.0 0.0 0.0 1.2328054460413384E-4 0.0 7 0.0 0.0 0.0 2.465610892082677E-4 0.0 8 0.0 0.0 0.0 3.6984163381240153E-4 1.2328054460413384E-4 9 0.0 0.0 0.0 4.931221784165354E-4 1.2328054460413384E-4 10-11 0.0 0.0 0.0 6.164027230206692E-4 1.2328054460413384E-4 12-13 0.0 0.0 0.0 6.164027230206692E-4 1.2328054460413384E-4 14-15 0.0 0.0 0.0 6.164027230206692E-4 1.2328054460413384E-4 16-17 0.0 0.0 0.0 7.396832676248031E-4 1.2328054460413384E-4 18-19 6.164027230206692E-5 0.0 0.0 8.0132353992687E-4 1.2328054460413384E-4 20-21 1.2328054460413384E-4 0.0 0.0 0.0010478846291351377 1.2328054460413384E-4 22-23 1.2328054460413384E-4 0.0 0.0 0.0028354525258950784 1.2328054460413384E-4 24-25 1.2328054460413384E-4 0.0 0.0 0.005979106413300492 1.2328054460413384E-4 26-27 1.2328054460413384E-4 0.0 0.0 0.01158837119278858 1.2328054460413384E-4 28-29 1.2328054460413384E-4 0.0 0.0 0.032484423503189266 1.2328054460413384E-4 30-31 1.2328054460413384E-4 6.164027230206692E-5 0.0 0.08900855320418463 1.2328054460413384E-4 32-33 1.2328054460413384E-4 1.2328054460413384E-4 0.0 0.19114648440870952 1.2328054460413384E-4 34-35 1.2328054460413384E-4 1.2328054460413384E-4 0.0 0.3510413507602711 1.2328054460413384E-4 36-37 1.2328054460413384E-4 1.2328054460413384E-4 0.0 0.5934725417243003 1.2328054460413384E-4 38-39 1.2328054460413384E-4 1.2328054460413384E-4 0.0 0.9623279311798688 1.2328054460413384E-4 40-41 1.2328054460413384E-4 1.2328054460413384E-4 0.0 1.3900497806839112 1.2328054460413384E-4 42-43 1.8492081690620076E-4 1.2328054460413384E-4 0.0 1.772096188412122 1.2328054460413384E-4 44-45 2.465610892082677E-4 1.2328054460413384E-4 0.0 2.214981544902473 1.2328054460413384E-4 46-47 3.6984163381240153E-4 1.2328054460413384E-4 0.0 2.7381841762024166 1.2328054460413384E-4 48-49 3.6984163381240153E-4 1.2328054460413384E-4 0.0 3.278461162930033 1.2328054460413384E-4 50-51 3.6984163381240153E-4 1.2328054460413384E-4 0.0 3.880563342776623 1.2328054460413384E-4 52-53 3.6984163381240153E-4 1.2328054460413384E-4 0.0 4.43396970750458 1.2328054460413384E-4 54-55 3.6984163381240153E-4 1.2328054460413384E-4 0.0 5.009135088355166 1.2328054460413384E-4 56-57 3.6984163381240153E-4 1.2328054460413384E-4 0.0 5.648961114850621 1.2328054460413384E-4 58-59 3.6984163381240153E-4 1.2328054460413384E-4 0.0 6.3527081037233195 1.2328054460413384E-4 60-61 3.6984163381240153E-4 1.2328054460413384E-4 0.0 7.141703589189776 1.2328054460413384E-4 62-63 3.6984163381240153E-4 1.2328054460413384E-4 0.0 7.894023112636503 1.2328054460413384E-4 64-65 3.6984163381240153E-4 1.2328054460413384E-4 0.0 8.637343156327127 1.2328054460413384E-4 66-67 3.6984163381240153E-4 1.2328054460413384E-4 0.0 9.444214320761184 1.2328054460413384E-4 68-69 3.6984163381240153E-4 1.2328054460413384E-4 0.0 10.346997748897255 1.2328054460413384E-4 70-71 3.6984163381240153E-4 1.2328054460413384E-4 0.0 11.347604289176708 1.2328054460413384E-4 72-73 3.6984163381240153E-4 1.2328054460413384E-4 0.0 12.351662684705076 1.2328054460413384E-4 74-75 3.6984163381240153E-4 1.2328054460413384E-4 0.0 13.336612595819803 1.2328054460413384E-4 76-77 3.6984163381240153E-4 1.2328054460413384E-4 0.0 14.352444283357865 1.8492081690620076E-4 78-79 3.6984163381240153E-4 1.2328054460413384E-4 0.0 15.452353302315949 2.465610892082677E-4 80-81 3.6984163381240153E-4 1.2328054460413384E-4 0.0 16.61760100991422 2.465610892082677E-4 82-83 3.6984163381240153E-4 1.2328054460413384E-4 0.0 17.80331328791678 2.465610892082677E-4 84-85 3.6984163381240153E-4 1.2328054460413384E-4 0.0 18.965972104078368 2.465610892082677E-4 86-87 3.6984163381240153E-4 1.2328054460413384E-4 0.0 20.126596791253988 2.465610892082677E-4 88-89 3.6984163381240153E-4 1.2328054460413384E-4 0.0 21.33363660347306 2.465610892082677E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3630 0.0 39.596012 1 GTATCAA 5780 0.0 36.148296 1 ATCAACG 7320 0.0 28.47291 3 TATCAAC 7345 0.0 28.440636 2 TCAACGC 7370 0.0 28.279743 4 CAACGCA 7545 0.0 27.623817 5 AACGCAG 7700 0.0 26.99278 6 GTACATG 4460 0.0 25.61106 1 CATGGGA 2930 0.0 24.953503 4 TACATGG 4660 0.0 24.145758 2 ACATGGG 4635 0.0 23.866274 3 ACGCAGA 8650 0.0 23.807348 7 ATGGGAG 980 0.0 23.738235 5 GTAGTAC 100 0.0016580013 23.738235 3 GACCGCT 100 0.0016633658 23.725065 7 CGCAGAG 8695 0.0 23.629566 8 TAAGGTG 250 3.8380676E-10 22.788708 5 TAAGGCG 230 3.6434358E-9 22.706139 5 GAGTACT 5205 0.0 22.700886 12-13 GCAGAGT 9075 0.0 22.378685 9 >>END_MODULE