FastQCFastQC Report
Thu 26 May 2016
SRR938801_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938801_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003452
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67490.6725782598470081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53270.5308674455778652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33690.33574102199208333No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31290.31182358498463303No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23480.23399225872288862No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19760.19692023136134068No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.14948398129656426No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12570.12526757632652086No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.11749440929909952No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11560.11520232158588552No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.10782777850858835No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10160.10125048333153952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG40150.024.195231
TATTCCG1201.7430993E-423.6841665
GAGTACT56500.023.37790912-13
TACATGG41200.022.8793642
GTATCAA117450.022.3117491
ACATGGG39850.021.9903183
GTACTTT61500.021.43877414-15
AGTACTT57950.021.32244112-13
AGAGTAC82200.020.50350410-11
GCCGTAT2150.019.90904894-95
GGTATCA90250.019.9000171
ATCAACG131950.019.636593
TCAACGC132700.019.5969984
TATCAAC133150.019.5307662
CAACGCA133800.019.3650825
AACGCAG134400.019.3375426
CATGGGG15400.019.070374
GTACTAG1559.778176E-418.3434491
CATGGGA24000.018.1578624
ACTTTTT75150.018.0194316-17