##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938801_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1003452 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.526162686406526 33.0 31.0 34.0 30.0 34.0 2 31.56330746263897 33.0 31.0 34.0 28.0 34.0 3 31.5566584151509 33.0 31.0 34.0 28.0 34.0 4 35.13471496394446 37.0 35.0 37.0 32.0 37.0 5 35.18057664940625 37.0 35.0 37.0 33.0 37.0 6 35.277136325404705 37.0 35.0 37.0 33.0 37.0 7 35.19466999916289 37.0 35.0 37.0 32.0 37.0 8 35.17196736864344 37.0 35.0 37.0 32.0 37.0 9 36.72082471309041 39.0 37.0 39.0 32.0 39.0 10-11 36.62586202429215 39.0 37.0 39.0 32.0 39.0 12-13 36.514285187532636 39.0 37.0 39.0 32.0 39.0 14-15 37.62995838365961 40.0 37.0 41.0 32.0 41.0 16-17 37.56725184662545 40.0 37.0 41.0 32.0 41.0 18-19 37.53860174677015 40.0 37.0 41.0 32.0 41.0 20-21 37.4442982823294 40.0 37.0 41.0 32.0 41.0 22-23 37.33898083814672 40.0 37.0 41.0 32.0 41.0 24-25 37.31393878331998 40.0 37.0 41.0 31.0 41.0 26-27 37.195760235666484 40.0 36.5 41.0 31.0 41.0 28-29 37.066686298896215 40.0 36.0 41.0 31.0 41.0 30-31 36.83165064198387 39.0 36.0 41.0 30.5 41.0 32-33 36.5628460554167 39.0 35.0 41.0 30.0 41.0 34-35 36.19586288133364 39.0 35.0 41.0 29.5 41.0 36-37 35.9071674579352 39.0 35.0 41.0 29.0 41.0 38-39 35.70788537966938 39.0 35.0 41.0 27.5 41.0 40-41 35.50198464899168 38.5 35.0 40.0 27.0 41.0 42-43 35.19256177674667 38.0 35.0 40.0 25.5 41.0 44-45 35.049371569342625 39.0 35.0 40.5 24.5 41.0 46-47 34.74871942056022 39.0 34.5 41.0 23.5 41.0 48-49 34.42137341895776 39.0 34.0 41.0 21.5 41.0 50-51 33.222090344132056 37.5 32.5 39.5 17.5 40.5 52-53 33.30397368284682 37.5 33.0 39.5 18.0 40.5 54-55 33.8219581006366 38.0 34.0 40.0 18.0 41.0 56-57 33.7180099297226 38.0 34.0 40.0 16.0 41.0 58-59 33.49906622339684 38.0 33.0 40.0 13.5 41.0 60-61 33.19561922244412 37.5 33.0 40.0 10.5 41.0 62-63 32.82998040763285 37.0 33.0 40.0 8.0 41.0 64-65 32.44099468634275 36.5 32.0 39.5 6.5 41.0 66-67 32.022564606976715 36.0 31.5 39.0 2.0 41.0 68-69 31.606906458903865 35.5 31.0 39.0 2.0 40.5 70-71 31.194110929072842 35.0 31.0 38.5 2.0 40.0 72-73 30.765093895871452 35.0 31.0 37.5 2.0 40.0 74-75 30.2901254868195 35.0 30.0 37.0 2.0 39.0 76-77 29.83015032109159 35.0 30.0 36.5 2.0 39.0 78-79 29.375310926681095 34.5 29.0 36.0 2.0 38.5 80-81 28.908911437717002 34.0 29.0 35.5 2.0 37.0 82-83 28.410725674970003 34.0 28.5 35.0 2.0 37.0 84-85 27.96267683954987 34.0 27.5 35.0 2.0 36.5 86-87 27.54254961871619 34.0 26.5 35.0 2.0 36.0 88-89 27.12264562729458 34.0 26.0 35.0 2.0 36.0 90-91 26.65851131892706 34.0 25.0 35.0 2.0 35.0 92-93 26.169354886930314 33.0 24.0 35.0 2.0 35.0 94-95 25.61937940230325 33.0 20.5 35.0 2.0 35.0 96-97 25.004093369687837 33.0 12.5 35.0 2.0 35.0 98-99 24.301062233171095 32.5 2.0 35.0 2.0 35.0 100-101 22.22156814675739 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3159130943597219 1101 2 0.18724526991977797 1101 3 0.33273905905004497 1101 4 0.15225228003161817 1101 5 0.06041866592043732 1101 6 -0.12958312207607747 1101 7 0.26674480792866717 1101 8 0.3825374269886197 1101 9 0.35340307748180777 1101 10-11 0.47780706699320774 1101 12-13 0.4941499776531728 1101 14-15 0.828175396920507 1101 16-17 0.7330983217434408 1101 18-19 0.712599725195119 1101 20-21 0.6549579917896935 1101 22-23 0.5733420113171235 1101 24-25 0.5873940691185098 1101 26-27 0.7391083083004659 1101 28-29 0.7920288459695541 1101 30-31 0.6901755124430693 1101 32-33 0.7003036255382113 1101 34-35 0.6622966940183446 1101 36-37 0.8115972000365801 1101 38-39 0.7235377560686445 1101 40-41 0.6220115684064922 1101 42-43 0.6172002770138576 1101 44-45 0.7392660575146905 1101 46-47 0.6394241604749595 1101 48-49 0.676537629092131 1101 50-51 0.8251757213685096 1101 52-53 0.6717718452453809 1101 54-55 0.7389453766351295 1101 56-57 0.700927755788392 1101 58-59 0.6662397857157742 1101 60-61 0.4679732662821081 1101 62-63 0.24356021698317676 1101 64-65 0.28323220036471497 1101 66-67 0.20080321941829027 1101 68-69 0.16494486316311807 1101 70-71 0.030495798855508127 1101 72-73 -0.058919208792698186 1101 74-75 -0.27621951335359185 1101 76-77 -0.094643387262618 1101 78-79 -0.2840838655004596 1101 80-81 -0.3303373313944924 1101 82-83 -0.6058553303483691 1101 84-85 -0.7364759190910668 1101 86-87 -0.3983495847056773 1101 88-89 -0.3875457136393621 1101 90-91 -0.7411552395640548 1101 92-93 -0.941159499143474 1101 94-95 -0.8556737777393657 1101 96-97 -1.1360533276605622 1101 98-99 -1.29123127774319 1101 100-101 -0.9256755574585576 1102 1 0.2108127523870813 1102 2 0.24342327567931932 1102 3 0.28510137006516345 1102 4 0.17954034698625776 1102 5 0.1274723016295809 1102 6 0.15792362738396548 1102 7 0.2396142283750322 1102 8 0.1853744500267709 1102 9 0.23676340865531387 1102 10-11 0.28629563790753565 1102 12-13 0.3591101558389198 1102 14-15 0.16222079828839497 1102 16-17 0.23456857912285045 1102 18-19 0.32832484218575786 1102 20-21 0.2480701678156052 1102 22-23 0.21126385256982871 1102 24-25 0.25133395392771973 1102 26-27 0.38492814271371145 1102 28-29 0.2734104604403953 1102 30-31 0.35474872158633985 1102 32-33 0.2596448782379923 1102 34-35 0.10160554930991594 1102 36-37 0.14389899089331237 1102 38-39 0.21828780106504553 1102 40-41 0.3168009852531455 1102 42-43 0.17339178169348202 1102 44-45 0.028284686024399264 1102 46-47 -0.08431706522698335 1102 48-49 -0.0543029036652527 1102 50-51 -0.07355875487338892 1102 52-53 0.16270844531738504 1102 54-55 0.05126314121395836 1102 56-57 -0.13234145767452787 1102 58-59 -0.030222372311584422 1102 60-61 0.040169990544171696 1102 62-63 -0.15095584673173335 1102 64-65 -0.01620309481167226 1102 66-67 -0.01279019310870666 1102 68-69 -0.1072597354689897 1102 70-71 -0.314944940885308 1102 72-73 -0.03584842945504363 1102 74-75 -0.10851567966028597 1102 76-77 -0.08664865184144688 1102 78-79 -0.20130173807065432 1102 80-81 -0.26415437603091974 1102 82-83 -0.22329143545995933 1102 84-85 -0.5209690346418192 1102 86-87 -0.5060158464047397 1102 88-89 -0.4359967125594508 1102 90-91 -0.38874005777859466 1102 92-93 -0.536275139892215 1102 94-95 -0.5964135185601016 1102 96-97 -0.5372119849679784 1102 98-99 -0.6238097964616927 1102 100-101 -0.6885152802807326 1103 1 0.16124885144709467 1103 2 0.2220250471905274 1103 3 0.304122519739785 1103 4 0.3270389912378846 1103 5 0.2871161917235767 1103 6 0.2278870221778888 1103 7 0.21890065281465354 1103 8 0.1626543668742073 1103 9 0.21820773258879456 1103 10-11 0.19745118743754375 1103 12-13 0.22368784022069832 1103 14-15 0.2571107567121089 1103 16-17 0.23451321939761982 1103 18-19 0.6540829314843819 1103 20-21 0.6316215506789504 1103 22-23 0.6093691942184165 1103 24-25 0.31479201611137597 1103 26-27 0.3685281065604187 1103 28-29 0.4259287630434301 1103 30-31 0.3822783220924819 1103 32-33 0.30178153767399607 1103 34-35 0.37047756666349585 1103 36-37 0.5457416788904155 1103 38-39 0.5800568267338875 1103 40-41 0.6490949115004341 1103 42-43 0.514968065135271 1103 44-45 0.5502003584755997 1103 46-47 0.5261527015843015 1103 48-49 0.8108182098560803 1103 50-51 0.8432355780984082 1103 52-53 0.7019110393159309 1103 54-55 0.8486397681120081 1103 56-57 0.8642477053768047 1103 58-59 0.7525743106240625 1103 60-61 0.7327992385557351 1103 62-63 0.6869678246348627 1103 64-65 0.44301373454479887 1103 66-67 0.3958459240279524 1103 68-69 0.37153748976600554 1103 70-71 0.2624242293966823 1103 72-73 0.42074163742854154 1103 74-75 0.383695545936245 1103 76-77 0.1449030021715636 1103 78-79 -0.1090769549903925 1103 80-81 -0.14462030155514327 1103 82-83 -0.015260885929951229 1103 84-85 0.26213495125839614 1103 86-87 0.11346738750704688 1103 88-89 -0.20335368110608698 1103 90-91 -0.36691363876196803 1103 92-93 -0.5102470307092801 1103 94-95 -0.5996944296230069 1103 96-97 -0.6371554954524292 1103 98-99 -0.7125627339165028 1103 100-101 -0.8811015962604891 1104 1 0.13938260791914558 1104 2 0.16892098533400457 1104 3 0.06838625378609464 1104 4 0.26137834910042557 1104 5 0.07196666385650019 1104 6 0.18443657664400348 1104 7 0.14471685346312313 1104 8 0.1200225296603179 1104 9 0.049228172573570816 1104 10-11 0.15744829823170647 1104 12-13 0.21278682074377997 1104 14-15 0.20883923556817052 1104 16-17 0.20981853507775128 1104 18-19 0.19067839222099536 1104 20-21 0.2829505853631744 1104 22-23 0.3819199043668675 1104 24-25 0.19436601876211057 1104 26-27 0.3098004119173794 1104 28-29 0.17281254817824276 1104 30-31 0.2688288396272114 1104 32-33 0.2861247936713909 1104 34-35 0.28046390026129586 1104 36-37 0.17638996164736653 1104 38-39 0.1817270628691361 1104 40-41 0.10155257087682656 1104 42-43 0.14340587612618094 1104 44-45 0.2737194111829737 1104 46-47 0.2823220732508247 1104 48-49 0.09616397441791236 1104 50-51 -0.02974599060277683 1104 52-53 0.22627323566269553 1104 54-55 0.22711541394123458 1104 56-57 0.08844893168417656 1104 58-59 0.11919733346713457 1104 60-61 -0.03503702143609644 1104 62-63 -0.06310899155202776 1104 64-65 -0.15238394400124378 1104 66-67 -0.001783322072768101 1104 68-69 0.23165521343869244 1104 70-71 0.015495730362044213 1104 72-73 0.13744812621796143 1104 74-75 0.08666798841582235 1104 76-77 0.0080956663403704 1104 78-79 0.017943769328923054 1104 80-81 -0.007034485262774837 1104 82-83 -0.21488322545291183 1104 84-85 -0.3264295261851622 1104 86-87 -0.24451897360699704 1104 88-89 -0.2846125952232974 1104 90-91 -0.2128822794135523 1104 92-93 0.013907647281850899 1104 94-95 -0.2928458652831445 1104 96-97 -0.19093599835769126 1104 98-99 -0.078928938463104 1104 100-101 0.1303646527707123 1105 1 0.22841777993528822 1105 2 0.23662231149199187 1105 3 0.31211583287508304 1105 4 0.39490708252345286 1105 5 0.25601913358550377 1105 6 0.16673044556578276 1105 7 0.29585561955960316 1105 8 0.2959546841865546 1105 9 0.2803928852393369 1105 10-11 0.2919860649047834 1105 12-13 0.289185052533135 1105 14-15 0.5122939732195277 1105 16-17 0.3387825942743632 1105 18-19 0.4703487746926527 1105 20-21 0.3914456843445322 1105 22-23 0.40337387323098284 1105 24-25 0.4144193533767577 1105 26-27 0.4341877845869959 1105 28-29 0.27313928275444255 1105 30-31 0.1728745824678839 1105 32-33 0.3711118203867443 1105 34-35 0.46534521873140733 1105 36-37 0.4446006093506085 1105 38-39 0.6827721330209684 1105 40-41 0.38188362988124425 1105 42-43 0.3759399910598731 1105 44-45 0.3989716694954737 1105 46-47 0.26605741824409535 1105 48-49 0.3467645894476945 1105 50-51 0.5322134558704121 1105 52-53 0.33832397562040484 1105 54-55 0.2932311164205714 1105 56-57 0.1941091277249214 1105 58-59 0.129251629065827 1105 60-61 0.3207312853100106 1105 62-63 0.35628847145008535 1105 64-65 0.2908819602847501 1105 66-67 0.05441022011443408 1105 68-69 0.008154348553048862 1105 70-71 -0.19553636650514505 1105 72-73 0.1700615223355868 1105 74-75 -0.020421671395823182 1105 76-77 0.08609153410896297 1105 78-79 0.033848055317776016 1105 80-81 0.2420525798919435 1105 82-83 0.14848886757034307 1105 84-85 0.11057796949041077 1105 86-87 -0.09375258882898407 1105 88-89 -0.2301728489230861 1105 90-91 -0.17791447237914682 1105 92-93 -0.13425206826686775 1105 94-95 -0.08566971690720848 1105 96-97 -0.0917143954362949 1105 98-99 0.13145449417191557 1105 100-101 0.2912453946503959 1106 1 0.012508689026198283 1106 2 0.20769669165727933 1106 3 0.30488442791640225 1106 4 0.19965914863915657 1106 5 0.03287863771773658 1106 6 0.16776350341702084 1106 7 0.10680603278274248 1106 8 0.21331005608737996 1106 9 0.10064081912363321 1106 10-11 0.20624226325188744 1106 12-13 0.11821397815296564 1106 14-15 0.18068240297159122 1106 16-17 0.3604768091503985 1106 18-19 0.29886117138686075 1106 20-21 0.10373907277428174 1106 22-23 0.12031602199131441 1106 24-25 -0.15531205158192307 1106 26-27 -0.1339940335948313 1106 28-29 -0.14318303129513765 1106 30-31 0.0055192105670514024 1106 32-33 0.024004382370222288 1106 34-35 0.38838240881405284 1106 36-37 0.24935267546631223 1106 38-39 0.31861924045898604 1106 40-41 0.21556131583165694 1106 42-43 0.2499069332086279 1106 44-45 0.23729811577647553 1106 46-47 0.4829995670044269 1106 48-49 0.6045125233319979 1106 50-51 0.6665109765315691 1106 52-53 0.52685703347165 1106 54-55 0.5525286370817284 1106 56-57 0.622828135989387 1106 58-59 0.5662351001402044 1106 60-61 0.9157726076240564 1106 62-63 0.6977140912848014 1106 64-65 0.7939811338384644 1106 66-67 0.5864350135028644 1106 68-69 0.8165221171480894 1106 70-71 0.6634512368006398 1106 72-73 0.6158259851455057 1106 74-75 0.5906320476124414 1106 76-77 0.20695930270400353 1106 78-79 0.5911827660615785 1106 80-81 0.7410195220407054 1106 82-83 0.7528277105455494 1106 84-85 0.7593383000689222 1106 86-87 0.8912680723280424 1106 88-89 1.0797445064488151 1106 90-91 1.2869615606787015 1106 92-93 1.3915743780141199 1106 94-95 1.1705286302002307 1106 96-97 1.3788434558033735 1106 98-99 1.3277354859074535 1106 100-101 1.1786420888652707 1107 1 -0.07097891427958558 1107 2 0.09650660901264985 1107 3 -0.11014862993483732 1107 4 -0.011084653013739398 1107 5 0.011638968296246333 1107 6 0.06354862738396605 1107 7 -0.08617743829163516 1107 8 0.12958278336010665 1107 9 -0.03906992467801729 1107 10-11 -0.009162695425800393 1107 12-13 -0.04082734416108025 1107 14-15 -0.042425035044935555 1107 16-17 -0.03453558754381447 1107 18-19 0.11472067551909504 1107 20-21 -0.001492332184390932 1107 22-23 -0.09042364743017117 1107 24-25 -0.2783327127389441 1107 26-27 0.02934480938037609 1107 28-29 -0.11140203955960715 1107 30-31 0.05297788825300387 1107 32-33 0.2041448782379902 1107 34-35 0.11910554930991424 1107 36-37 0.09829482422664171 1107 38-39 0.10489196773171017 1107 40-41 0.22730098525314446 1107 42-43 0.28672511502681175 1107 44-45 0.2509096860244071 1107 46-47 0.48899543477301677 1107 48-49 0.5298637630014156 1107 50-51 0.48237874512660994 1107 52-53 0.31868761198404627 1107 54-55 0.19588814121396325 1107 56-57 0.2776793756588063 1107 58-59 0.3951317943550876 1107 60-61 0.27758665721083986 1107 62-63 -0.011893346731724819 1107 64-65 -0.08891142814501052 1107 66-67 -0.06204019310870379 1107 68-69 -0.1136555688023222 1107 70-71 -0.1825074408853098 1107 72-73 -0.2092442627883777 1107 74-75 0.013359320339713321 1107 76-77 0.026913848158550024 1107 78-79 -0.030676738070653187 1107 80-81 0.08738729063574624 1107 82-83 0.0256252312067069 1107 84-85 0.050218465358177866 1107 86-87 0.15633832026192707 1107 88-89 0.026190787440548036 1107 90-91 0.03915577555473604 1107 92-93 0.05051652677445162 1107 94-95 0.1650864814399 1107 96-97 0.24499634836535833 1107 98-99 0.362169370204974 1107 100-101 0.47502638638593453 1108 1 0.09649917336981417 1108 2 0.08385322255846717 1108 3 0.022476868073130873 1108 4 -0.11915238209740409 1108 5 0.1203839882165667 1108 6 0.08908647598953934 1108 7 0.014942083620717028 1108 8 -0.10672797361200281 1108 9 0.04381852153711918 1108 10-11 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Count 2 4765.0 3 1114.0 4 993.0 5 1114.0 6 1319.0 7 1652.0 8 2058.0 9 2543.0 10 3128.0 11 4204.0 12 5755.0 13 7685.0 14 8206.0 15 8631.0 16 10384.0 17 13431.0 18 11521.0 19 7865.0 20 7781.0 21 8170.0 22 8942.0 23 9709.0 24 11001.0 25 12544.0 26 14471.0 27 16643.0 28 19757.0 29 23091.0 30 26736.0 31 32054.0 32 38745.0 33 47216.0 34 61669.0 35 84263.0 36 119605.0 37 174677.0 38 162636.0 39 27369.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.12109622564866 19.65064020352374 14.756069931842703 21.472193638984898 2 15.83294100749276 22.150218239560807 38.64595718195674 23.370883570989697 3 19.313160892140807 25.88981690008797 31.70008238398918 23.09693982378204 4 12.954578327073952 17.994403962114525 37.8200390026883 31.23097870812323 5 12.925741537049415 37.640650098272985 34.467879199050195 14.965729165627401 6 29.344581647648084 36.67639030401155 18.214074217340244 15.764953831000126 7 25.3888035349492 34.703338840074856 22.5519509717222 17.355906653253747 8 24.975174080543656 33.160249814553374 21.524670383575412 20.339905721327558 9 25.651192910634148 17.20884136766215 22.490769885748982 34.64919583595472 10-11 23.91653410555271 26.81908660698218 28.265103617011622 20.999275670453486 12-13 25.60842039771852 25.228240218436593 28.288675705708833 20.87466367813605 14-15 22.56314540017927 26.387912614921987 28.147897778881177 22.90104420601757 16-17 20.64134750174978 29.300874791372127 28.66586838048938 21.39190932638871 18-19 20.237421032480377 27.88814729436539 31.458685629506732 20.415746043647502 20-21 21.509338956898464 26.619753769608316 31.2737026041394 20.597204669353815 22-23 21.168783250813973 26.872491386574527 31.084419048758722 20.87430631385278 24-25 21.322998101539465 26.920838633846454 31.212895369242343 20.543267895371738 26-27 20.92335066440196 26.752015447358 31.770312308316818 20.554321579923222 28-29 20.438714721740617 27.777869195377196 31.258585982490704 20.524830100391483 30-31 20.844767618105525 27.067345278172215 31.020549802958026 21.067337300764237 32-33 20.73553268264181 27.291354457376514 30.85445388675016 21.118658973231515 34-35 21.450136864300685 27.18554321158566 30.709230456292637 20.65508946782102 36-37 21.229376960671914 26.82754898613299 31.02179731471181 20.92127673848329 38-39 20.862464562010473 27.35762737654766 30.95688578155783 20.823022279884036 40-41 20.970544286656505 27.264725104362558 30.814303200385122 20.95042740859581 42-43 20.756911904348343 27.3198484894749 30.177564849237815 21.745674756938936 44-45 20.93007422551802 27.167280498568005 30.2211546981544 21.681490577759575 46-47 21.268168433657493 27.49987735024465 29.850797824143278 21.381156391954576 48-49 21.15291794427076 27.55724434065615 29.585605649430107 21.70423206564298 50-51 20.509923211634103 27.88041481137337 29.80372857630625 21.80593340068628 52-53 21.01563196246166 28.116153762195108 29.073888083920536 21.794326191422698 54-55 20.590314386441303 27.582581635442295 29.369534983614336 22.45756899450207 56-57 20.926491426260434 27.77897625660505 29.09365469694975 22.200877620184762 58-59 21.403332484290623 27.33238620867735 29.065984630158496 22.19829667687353 60-61 20.892530545761613 27.38743191486807 29.81193295740602 21.908104581964295 62-63 20.68290299846233 27.2897912929267 29.813036583803175 22.214269124807792 64-65 20.497741635036203 27.61074000260388 29.43801263883186 22.453505723528057 66-67 20.66169088209594 27.401884321771853 29.00378316529002 22.932641630842184 68-69 20.56479944311316 27.213537558562013 29.306320315101797 22.915342683223034 70-71 20.82093887403223 27.201756758110236 29.16343659545487 22.81386777240267 72-73 20.791613170719007 26.857703116017937 29.421252295902526 22.92943141736053 74-75 20.443399206992567 27.607403739321963 28.941166338792122 23.008030714893348 76-77 20.651192268875253 27.101539096166476 29.048087243483973 23.199181391474298 78-79 20.72827533466162 27.12271835272461 29.08546927699477 23.063537035618996 80-81 20.941083198653825 26.9731069749889 28.97250335718608 23.113306469171196 82-83 20.91279515044726 26.71739141316808 29.186017990599233 23.183795445785425 84-85 20.777770436133704 26.799637189298288 29.025857270430127 23.396735104137882 86-87 20.949240888636133 26.519061262066764 29.121621520145098 23.41007632915201 88-89 21.01704724955453 26.384159661067695 28.844793376761285 23.753999712616487 90-91 21.217003724653058 26.114028758787782 29.044633250101516 23.624334266457645 92-93 21.55872442056669 26.097706300288902 28.952438651919426 23.391130627224978 94-95 21.398267000147715 25.759758451566384 29.063724422479424 23.778250125806476 96-97 21.390644778797927 25.5038836546011 29.19485771282988 23.91061385377109 98-99 21.543825987860966 25.54624137665144 28.79269615270224 24.117236482785348 100-101 21.918507055749608 24.89645542889956 29.283000467095782 23.90203704825505 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2161.0 1 1384.0 2 750.5 3 850.0 4 744.5 5 770.5 6 1094.5 7 1769.5 8 2596.0 9 2930.5 10 2585.0 11 2246.5 12 2251.0 13 2375.0 14 2560.0 15 2710.5 16 2847.5 17 2973.0 18 3060.0 19 3247.0 20 3493.0 21 3832.5 22 4298.0 23 4832.0 24 5290.5 25 6005.5 26 7120.5 27 8524.0 28 9850.0 29 11269.5 30 13033.5 31 14869.5 32 16674.5 33 18134.0 34 19591.0 35 21362.5 36 23518.0 37 25556.5 38 26899.0 39 27353.5 40 27647.0 41 28884.5 42 30345.5 43 31909.0 44 33397.5 45 34435.0 46 35843.5 47 38845.5 48 44545.5 49 45882.5 50 40759.5 51 36276.0 52 33411.5 53 31481.5 54 29734.5 55 26856.0 56 24036.0 57 21183.5 58 18737.0 59 16498.5 60 13685.0 61 11063.5 62 8765.0 63 6842.0 64 5015.5 65 3721.5 66 2768.5 67 2140.0 68 1659.5 69 1158.0 70 843.0 71 629.5 72 506.0 73 412.5 74 325.0 75 269.5 76 197.0 77 122.0 78 76.0 79 49.0 80 33.5 81 24.5 82 20.5 83 16.0 84 15.0 85 11.5 86 6.0 87 7.5 88 7.0 89 4.0 90 3.0 91 3.0 92 2.0 93 2.0 94 2.5 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14998226123421948 2 0.08809589297744187 3 0.08271446965076557 4 0.095370780067208 5 0.11380713776045093 6 0.03966308303735505 7 0.04374897852612781 8 0.04663900216452805 9 0.04833315395255578 10-11 0.04539330232039001 12-13 0.0504757576844732 14-15 0.04873177790267995 16-17 0.04683831413959014 18-19 0.05062524166576976 20-21 0.052767845397687176 22-23 0.06547398380789515 24-25 0.07947565005600667 26-27 0.08181756576298617 28-29 0.07249973092883367 30-31 0.061786712269246556 32-33 0.05172145752861124 34-35 0.2998150384871424 36-37 0.47346559675998456 38-39 0.4508436875904378 40-41 0.42762384249570484 42-43 0.45617528292334864 44-45 0.46554294575126665 46-47 0.4656924297325632 48-49 0.48213566767518523 50-51 0.4716717889844258 52-53 0.4776511482362883 54-55 0.46967866923380497 56-57 0.4485017718834583 58-59 0.4272750465393462 60-61 0.395434958523178 62-63 0.45243818339093456 64-65 0.4924998903784137 66-67 0.49324731028489655 68-69 0.5027146291003456 70-71 0.5004723693808971 72-73 0.4917026424781654 74-75 0.4831820555442612 76-77 0.46678864559540467 78-79 0.44052929288097487 80-81 0.4460103721951822 82-83 0.45811857468020395 84-85 0.4568230468423004 86-87 0.4669381295767013 88-89 0.4770033843173365 90-91 0.48188652770635765 92-93 0.48303257156296464 94-95 0.48776623097068916 96-97 0.4891115868023582 98-99 0.483829819463213 100-101 0.471123681053005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1003452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.22495137201707 #Duplication Level Percentage of deduplicated Percentage of total 1 82.30226624491081 52.035567811412875 2 10.84234732153727 13.710137643254269 3 2.9761843754511554 5.645073372361689 4 1.2692612404780836 3.2099592083045163 5 0.6788824467442485 2.1461154841360535 6 0.4168328762081348 1.58125429971104 7 0.28153869934587955 1.246018940283892 8 0.19474359319242107 0.9850123367682158 9 0.1365027939563247 0.7767344259029785 >10 0.81209599621245 9.450068557231019 >50 0.05857116208921066 2.482054241496941 >100 0.02714358520895332 2.9406060059695296 >500 0.0017359265788900588 0.7893299032191351 >1k 0.0015781150717182352 1.797169791023014 >5k 3.1562301434364705E-4 1.204897978924823 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6749 0.6725782598470081 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5327 0.5308674455778652 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3369 0.33574102199208333 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3129 0.31182358498463303 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2348 0.23399225872288862 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1976 0.19692023136134068 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1500 0.14948398129656426 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1257 0.12526757632652086 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1179 0.11749440929909952 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1156 0.11520232158588552 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1082 0.10782777850858835 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1016 0.10125048333153952 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.965598753104284E-5 0.0 0.0 9.965598753104284E-5 0.0 3 9.965598753104284E-5 0.0 0.0 9.965598753104284E-5 0.0 4 9.965598753104284E-5 0.0 0.0 9.965598753104284E-5 0.0 5 9.965598753104284E-5 0.0 0.0 9.965598753104284E-5 0.0 6 9.965598753104284E-5 0.0 0.0 9.965598753104284E-5 0.0 7 9.965598753104284E-5 0.0 0.0 9.965598753104284E-5 0.0 8 9.965598753104284E-5 0.0 0.0 9.965598753104284E-5 0.0 9 9.965598753104284E-5 0.0 0.0 9.965598753104284E-5 0.0 10-11 9.965598753104284E-5 0.0 0.0 4.982799376552142E-4 0.0 12-13 9.965598753104284E-5 9.965598753104284E-5 0.0 4.982799376552142E-4 0.0 14-15 9.965598753104284E-5 9.965598753104284E-5 0.0 4.982799376552142E-4 0.0 16-17 9.965598753104284E-5 9.965598753104284E-5 0.0 4.982799376552142E-4 0.0 18-19 9.965598753104284E-5 9.965598753104284E-5 0.0 5.481079314207356E-4 0.0 20-21 9.965598753104284E-5 9.965598753104284E-5 0.0 8.969038877793856E-4 0.0 22-23 9.965598753104284E-5 9.965598753104284E-5 0.0 0.001993119750620857 0.0 24-25 9.965598753104284E-5 9.965598753104284E-5 0.0 0.004335035457600363 0.0 26-27 9.965598753104284E-5 9.965598753104284E-5 0.0 0.008221618971311033 0.0 28-29 9.965598753104284E-5 9.965598753104284E-5 0.0 0.025910556758071135 0.0 30-31 9.965598753104284E-5 9.965598753104284E-5 0.0 0.07120420309093012 0.0 32-33 9.965598753104284E-5 9.965598753104284E-5 0.0 0.16064545190004106 0.0 34-35 9.965598753104284E-5 9.965598753104284E-5 0.0 0.3179524282177922 0.0 36-37 9.965598753104284E-5 9.965598753104284E-5 0.0 0.5576250782299502 0.0 38-39 9.965598753104284E-5 9.965598753104284E-5 0.0 0.8831513615001017 0.0 40-41 9.965598753104284E-5 9.965598753104284E-5 0.0 1.2221810310807095 0.0 42-43 9.965598753104284E-5 9.965598753104284E-5 0.0 1.5670904039256486 0.0 44-45 9.965598753104284E-5 9.965598753104284E-5 0.0 1.9884359192068977 0.0 46-47 1.4948398129656425E-4 9.965598753104284E-5 0.0 2.48417462918007 0.0 48-49 2.989679625931285E-4 9.965598753104284E-5 0.0 2.965363564973711 0.0 50-51 2.989679625931285E-4 9.965598753104284E-5 0.0 3.4510868482000134 0.0 52-53 3.4879595635864993E-4 9.965598753104284E-5 0.0 3.9298342122991436 0.0 54-55 3.9862395012417136E-4 9.965598753104284E-5 0.0 4.464986865340844 0.0 56-57 3.9862395012417136E-4 9.965598753104284E-5 0.0 5.0969054822751865 0.0 58-59 3.9862395012417136E-4 9.965598753104284E-5 0.0 5.734404834511267 0.0 60-61 3.9862395012417136E-4 9.965598753104284E-5 0.0 6.411866237747296 0.0 62-63 3.9862395012417136E-4 9.965598753104284E-5 0.0 7.082102581887325 0.0 64-65 3.9862395012417136E-4 9.965598753104284E-5 0.0 7.7957391085971235 0.0 66-67 5.97935925186257E-4 9.965598753104284E-5 0.0 8.605693147255673 0.0 68-69 5.97935925186257E-4 9.965598753104284E-5 0.0 9.4863531090675 0.0 70-71 5.97935925186257E-4 9.965598753104284E-5 0.0 10.412157233230886 0.0 72-73 5.97935925186257E-4 9.965598753104284E-5 0.0 11.31807998788183 0.0 74-75 5.97935925186257E-4 9.965598753104284E-5 0.0 12.259231134125 0.0 76-77 5.97935925186257E-4 9.965598753104284E-5 0.0 13.220213821886846 0.0 78-79 5.97935925186257E-4 9.965598753104284E-5 0.0 14.303773374311875 0.0 80-81 5.97935925186257E-4 9.965598753104284E-5 0.0 15.435566424701928 0.0 82-83 5.97935925186257E-4 9.965598753104284E-5 0.0 16.559436824083264 0.0 84-85 5.97935925186257E-4 9.965598753104284E-5 0.0 17.702491001064324 0.0 86-87 5.97935925186257E-4 9.965598753104284E-5 0.0 18.857055444605223 0.0 88-89 5.97935925186257E-4 9.965598753104284E-5 0.0 20.071612792639808 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4015 0.0 24.19523 1 TATTCCG 120 1.7430993E-4 23.684166 5 GAGTACT 5650 0.0 23.377909 12-13 TACATGG 4120 0.0 22.879364 2 GTATCAA 11745 0.0 22.311749 1 ACATGGG 3985 0.0 21.990318 3 GTACTTT 6150 0.0 21.438774 14-15 AGTACTT 5795 0.0 21.322441 12-13 AGAGTAC 8220 0.0 20.503504 10-11 GCCGTAT 215 0.0 19.909048 94-95 GGTATCA 9025 0.0 19.900017 1 ATCAACG 13195 0.0 19.63659 3 TCAACGC 13270 0.0 19.596998 4 TATCAAC 13315 0.0 19.530766 2 CAACGCA 13380 0.0 19.365082 5 AACGCAG 13440 0.0 19.337542 6 CATGGGG 1540 0.0 19.07037 4 GTACTAG 155 9.778176E-4 18.343449 1 CATGGGA 2400 0.0 18.157862 4 ACTTTTT 7515 0.0 18.01943 16-17 >>END_MODULE