FastQCFastQC Report
Thu 26 May 2016
SRR938801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003452
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62580.6236471699692661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54690.5450185958072733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36440.3631464185631201No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33080.3296620067526897No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21070.20997516572790728No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT20280.2021023427129549No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20110.20040819092492715No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.13872113464321162No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.12546688830158292No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11850.11809234522428577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11560.11520232158588552No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT11240.11201332998489215RNA PCR Primer, Index 17 (95% over 21bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11000.10962158628414712No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10750.10713018659587105No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10230.10194807524425682No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGA350.00843347727.11612392-93
AGTACTT55650.021.48931712-13
GAGTACT55150.021.46902312-13
GTACATG40000.021.0820851
TACATGG41550.020.2147252
AGAGTAC84900.020.09405710-11
GTATCAA115600.020.0711121
GTACTTT60100.019.77973614-15
ACATGGG40300.019.6642343
GGTATCA91900.019.2335151
CATGGGA22450.019.0235584
ACGGGGC1507.771458E-418.9812858
TAGGGTG2854.3190084E-818.3152755
TAAGGTG2603.313162E-718.2512365
TACTTTT67350.017.9675914-15
CATGGGG14700.017.7546024
CAACGCA132350.017.4252075
ATCAACG132100.017.386343
TCAACGC132600.017.320784
TATCAAC133800.017.2718332
Produced by FastQC (version 0.11.5)