##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938801_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1003452 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9402901185109 31.0 31.0 34.0 30.0 34.0 2 32.1596259711476 33.0 31.0 34.0 30.0 34.0 3 32.235740224744184 34.0 31.0 34.0 30.0 34.0 4 35.890600646568046 37.0 35.0 37.0 35.0 37.0 5 35.79667188864041 37.0 35.0 37.0 35.0 37.0 6 35.83242447072705 37.0 35.0 37.0 35.0 37.0 7 35.76805666838075 37.0 35.0 37.0 33.0 37.0 8 35.743627996157265 37.0 35.0 37.0 33.0 37.0 9 37.37486994893627 39.0 37.0 39.0 34.0 39.0 10-11 37.311796179588065 39.0 37.0 39.0 34.0 39.0 12-13 37.244537357043484 39.0 37.0 39.0 33.0 39.0 14-15 38.43299181226406 40.0 38.0 41.0 33.0 41.0 16-17 38.37935845461467 40.0 38.0 41.0 33.0 41.0 18-19 38.42674936120512 40.0 38.0 41.0 33.5 41.0 20-21 38.47706816070924 40.0 38.0 41.0 34.0 41.0 22-23 38.41614696069169 40.0 38.0 41.0 34.0 41.0 24-25 38.32754282217785 40.0 38.0 41.0 33.0 41.0 26-27 38.15246817984318 40.0 37.5 41.0 33.0 41.0 28-29 37.9719333859517 40.0 37.0 41.0 33.0 41.0 30-31 37.70057710782379 40.0 37.0 41.0 32.5 41.0 32-33 37.420382340161765 40.0 36.0 41.0 32.0 41.0 34-35 37.29685874361704 40.0 36.0 41.0 31.5 41.0 36-37 37.16185527558867 40.0 36.0 41.0 31.0 41.0 38-39 37.02691459083245 40.0 35.5 41.0 31.0 41.0 40-41 36.83150614080195 40.0 35.0 41.0 31.0 41.0 42-43 36.60571706469268 39.5 35.0 41.0 30.0 41.0 44-45 36.28568581257499 39.0 35.0 41.0 30.0 41.0 46-47 35.87735088474585 39.0 35.0 41.0 28.5 41.0 48-49 35.50300263490431 39.0 35.0 41.0 27.0 41.0 50-51 35.20548666004951 39.0 35.0 41.0 26.0 41.0 52-53 34.99408990165947 39.0 35.0 40.0 26.0 41.0 54-55 34.74019933190626 38.0 34.0 40.0 24.0 41.0 56-57 34.44887000075739 38.0 34.0 40.0 23.5 41.0 58-59 34.117410199989635 38.0 34.0 40.0 23.0 41.0 60-61 33.75194628143648 37.5 33.0 40.0 22.0 41.0 62-63 33.37659549236037 37.0 33.0 40.0 20.5 41.0 64-65 32.99304700174996 36.0 33.0 39.5 19.5 41.0 66-67 32.56806802916333 36.0 32.0 39.0 17.5 40.5 68-69 32.10013084831163 35.0 32.0 39.0 13.5 40.0 70-71 31.620058059578334 35.0 31.0 38.0 9.0 40.0 72-73 31.29154508636188 35.0 31.0 37.0 7.5 39.5 74-75 30.883027788075562 35.0 31.0 37.0 4.0 39.0 76-77 28.831786174126915 32.5 28.0 35.0 2.0 37.0 78-79 29.847266735229987 34.0 30.0 36.0 2.0 38.0 80-81 29.86910534833754 34.0 30.0 35.5 2.0 37.0 82-83 29.56251270613841 34.0 30.0 35.0 2.0 37.0 84-85 29.186310854928784 34.0 29.5 35.0 2.0 36.0 86-87 28.68046204502059 34.0 29.0 35.0 2.0 36.0 88-89 28.17527644570941 34.0 28.5 35.0 2.0 35.5 90-91 27.767070074104193 34.0 27.0 35.0 2.0 35.0 92-93 27.492892534969286 33.5 27.0 35.0 2.0 35.0 94-95 27.136527706357654 33.0 26.5 35.0 2.0 35.0 96-97 26.664810075618963 33.0 25.5 35.0 2.0 35.0 98-99 25.988807636040388 33.0 24.5 35.0 2.0 35.0 100-101 23.22152230500313 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.009563989310674259 1101 2 0.023581257344133633 1101 3 0.04024888158784279 1101 4 0.1827239542505481 1101 5 0.08551609068069155 1101 6 0.17847573182391585 1101 7 0.0643502688967672 1101 8 0.11376544174932945 1101 9 0.32297368600249143 1101 10-11 0.1033710476129599 1101 12-13 0.2145058352148581 1101 14-15 0.2220267511863696 1101 16-17 0.09744890242740212 1101 18-19 0.13091885173958673 1101 20-21 0.19133781329821886 1101 22-23 0.1310198281372763 1101 24-25 -0.06666343756256055 1101 26-27 0.10561486453156022 1101 28-29 0.12678467987187503 1101 30-31 -0.014727254061064343 1101 32-33 0.1820309804070277 1101 34-35 -0.1131708367716655 1101 36-37 -0.092964353568604 1101 38-39 -0.0017735224056210086 1101 40-41 0.017345014593381336 1101 42-43 -0.06334377855990425 1101 44-45 -0.008163020435823398 1101 46-47 -0.05057890356805217 1101 48-49 -0.0013131866322169117 1101 50-51 -0.31431977153796 1101 52-53 -0.18370121259582817 1101 54-55 0.05173517418371887 1101 56-57 -0.24836805441413645 1101 58-59 -0.05010817585686311 1101 60-61 0.133369131142679 1101 62-63 -0.04793609248630304 1101 64-65 -0.1765749022366876 1101 66-67 -0.31147232597845687 1101 68-69 -0.21289685220460086 1101 70-71 -0.15995061173998337 1101 72-73 -0.12588126384556375 1101 74-75 -0.13523671793934255 1101 76-77 -0.24656381685685957 1101 78-79 -0.17171217861436006 1101 80-81 -0.3014747783498315 1101 82-83 -0.2620603587698902 1101 84-85 -0.06283347866043343 1101 86-87 -0.12852496979103378 1101 88-89 -0.1807354779718544 1101 90-91 0.05148557545556187 1101 92-93 -0.3164370530003673 1101 94-95 0.02321686009114643 1101 96-97 -0.20115379485765672 1101 98-99 -0.28341690072953085 1101 100-101 0.4234875719375033 1102 1 0.04844087915948592 1102 2 0.03961347476673893 1102 3 -0.004223310636774613 1102 4 -0.029557813208057837 1102 5 0.08246983438119315 1102 6 0.06030843520764506 1102 7 -0.060971455365290694 1102 8 -0.04275903629243061 1102 9 0.008453347629561847 1102 10-11 0.16625464192541273 1102 12-13 0.12255794104639506 1102 14-15 0.25464014211510033 1102 16-17 0.23776342726541344 1102 18-19 0.13578183770071206 1102 20-21 0.11533101884177199 1102 22-23 0.11946025290329487 1102 24-25 -0.03338297499956866 1102 26-27 0.13876146280369284 1102 28-29 0.1766440661209785 1102 30-31 -0.021979548877474997 1102 32-33 0.020355539442299175 1102 34-35 0.22196010275316524 1102 36-37 0.27000698372440723 1102 38-39 0.22474060646406713 1102 40-41 0.15152203043211898 1102 42-43 0.12860861524859502 1102 44-45 0.1089187380234975 1102 46-47 0.017366785776800953 1102 48-49 -0.03150239649542641 1102 50-51 0.05371028605743078 1102 52-53 0.11257983492037482 1102 54-55 -0.009471179308000899 1102 56-57 -0.05954403533566932 1102 58-59 0.03411721111218213 1102 60-61 -0.19841857584076905 1102 62-63 -0.35246722999530533 1102 64-65 -0.23299813837779482 1102 66-67 -0.17025449840465967 1102 68-69 -0.06731437380287275 1102 70-71 -0.030878437513923274 1102 72-73 -0.14640187219689693 1102 74-75 -0.22845126077591615 1102 76-77 -0.25017252815995406 1102 78-79 -0.27419485500024976 1102 80-81 -0.27935679778827094 1102 82-83 -0.2335056431471365 1102 84-85 -0.05344610465035515 1102 86-87 -0.14736608750161295 1102 88-89 0.09254453462714096 1102 90-91 0.05947356141668436 1102 92-93 0.07308553519257899 1102 94-95 0.012486343532469135 1102 96-97 0.1487224650415513 1102 98-99 3.708962467108279E-4 1102 100-101 0.31642651182231063 1103 1 0.0842326362093786 1103 2 0.11661546319768235 1103 3 0.012201580903351328 1103 4 0.08980453314045889 1103 5 0.05245808456196244 1103 6 0.12430201799867291 1103 7 0.09399284326087809 1103 8 0.07953850528385686 1103 9 0.0657246780706302 1103 10-11 0.01196256853423705 1103 12-13 0.1720495354064795 1103 14-15 0.18641888398060047 1103 16-17 0.1250804012350457 1103 18-19 0.13175865440353363 1103 20-21 0.38027701486490884 1103 22-23 0.19521495283098744 1103 24-25 0.2072680101775859 1103 26-27 0.24995316562365844 1103 28-29 0.1885283304015246 1103 30-31 0.08696500101407167 1103 32-33 0.042590942551484545 1103 34-35 -0.01848864995832855 1103 36-37 0.019680474324545116 1103 38-39 -0.0016399972958751619 1103 40-41 0.033542547424168845 1103 42-43 0.1657267461235037 1103 44-45 0.08904911582538233 1103 46-47 0.09565334579126272 1103 48-49 0.023630240887072773 1103 50-51 -0.20567601184567508 1103 52-53 -0.04400232343103738 1103 54-55 -0.34819361242441715 1103 56-57 -0.27612370814839693 1103 58-59 -0.44456477551110396 1103 60-61 -0.4034732576918145 1103 62-63 -0.44948006441323685 1103 64-65 -0.3135002171919652 1103 66-67 -0.39288984728390375 1103 68-69 -0.31322340308704355 1103 70-71 -0.30173436665347353 1103 72-73 -0.21476096474931694 1103 74-75 -0.20905502071807192 1103 76-77 -0.24848236185482264 1103 78-79 -0.3782474128455142 1103 80-81 -0.31161683032623344 1103 82-83 -0.23487042984272577 1103 84-85 -0.17030596546018728 1103 86-87 -0.17725694975758088 1103 88-89 -0.1303461468623759 1103 90-91 -0.28823861320370625 1103 92-93 -0.21696553132947471 1103 94-95 -0.442420335787844 1103 96-97 -0.18770875332431913 1103 98-99 -0.26672509073810957 1103 100-101 0.07179414558948238 1104 1 -0.020269480248547467 1104 2 0.0036154127512375567 1104 3 -0.0017193465775804384 1104 4 -0.020913961904319933 1104 5 0.05210954544532598 1104 6 -0.04589972194528258 1104 7 -0.053675384188409225 1104 8 -0.13593204545381354 1104 9 -0.047091930735483345 1104 10-11 -0.11502949302877852 1104 12-13 0.03503812075040713 1104 14-15 0.05728284824617447 1104 16-17 0.05423409322736461 1104 18-19 0.12418366116794033 1104 20-21 -0.004761255647309781 1104 22-23 0.04651715917531618 1104 24-25 -0.010451993674685411 1104 26-27 -0.045970302523997475 1104 28-29 0.045243211645995984 1104 30-31 -0.15828042097049178 1104 32-33 -0.10938701693542896 1104 34-35 0.02690856998361113 1104 36-37 0.04890387414019415 1104 38-39 0.25286120195384854 1104 40-41 0.1075451981558686 1104 42-43 0.1306148696531082 1104 44-45 0.026035765860008553 1104 46-47 -0.15431111239092132 1104 48-49 -0.06208401206976788 1104 50-51 -0.03878883304052039 1104 52-53 -0.15992941455108678 1104 54-55 -0.1330727473136406 1104 56-57 0.049151568963132775 1104 58-59 -0.10354905914856971 1104 60-61 -0.1432234560380934 1104 62-63 -0.38132117643646524 1104 64-65 -0.05635745832141481 1104 66-67 -0.053718865916991376 1104 68-69 -0.046400658158056274 1104 70-71 -0.19840398367318812 1104 72-73 -0.43800026895517874 1104 74-75 -0.25489594012757166 1104 76-77 -0.3883772497949103 1104 78-79 -0.4353195466845321 1104 80-81 -0.2747783094161811 1104 82-83 -0.38029035068765893 1104 84-85 -0.27589038759608897 1104 86-87 -0.28375284754389796 1104 88-89 0.22698670340797023 1104 90-91 -0.2544039491541419 1104 92-93 0.11637085936452607 1104 94-95 0.10787592246129307 1104 96-97 0.16355905771244394 1104 98-99 0.23936402521076872 1104 100-101 0.4174692796165296 1105 1 0.02261443287849474 1105 2 0.020312510579408638 1105 3 0.011350036470659575 1105 4 -0.02331642202348405 1105 5 -0.1200267220926321 1105 6 -0.05968984302927538 1105 7 -0.09780857657741393 1105 8 -0.06717363684339261 1105 9 0.05585348537060497 1105 10-11 -0.020672871848695706 1105 12-13 -0.10004019944948084 1105 14-15 0.014867414842370863 1105 16-17 -0.07688016777591145 1105 18-19 -0.24137467056375073 1105 20-21 -0.05284649493233218 1105 22-23 0.05460143747629331 1105 24-25 -0.11230429042656453 1105 26-27 -0.12252469422109158 1105 28-29 0.025555050969458648 1105 30-31 -0.159854032348548 1105 32-33 -0.11970558314722979 1105 34-35 0.07343221019118573 1105 36-37 0.1428160401982339 1105 38-39 0.16372993153293436 1105 40-41 0.1944541929665533 1105 42-43 -0.02474021395250503 1105 44-45 -0.03591166831258619 1105 46-47 -0.06806279411300409 1105 48-49 0.028152390005949712 1105 50-51 0.18874816484531465 1105 52-53 0.20764698368069645 1105 54-55 0.11961800801982037 1105 56-57 0.3414588916615813 1105 58-59 0.08959672213146774 1105 60-61 -0.053695779697513046 1105 62-63 0.10144117220855264 1105 64-65 -0.04090963975520623 1105 66-67 0.1283035250113116 1105 68-69 0.030141721238450714 1105 70-71 0.059428036315278376 1105 72-73 -0.21968871792692113 1105 74-75 -0.07879561339299457 1105 76-77 -0.11890531052910092 1105 78-79 -0.3370349032096165 1105 80-81 -0.08253345068083817 1105 82-83 0.02128085823027348 1105 84-85 0.13932679479868426 1105 86-87 0.23436170175458315 1105 88-89 0.2153708087318229 1105 90-91 0.3770226316646159 1105 92-93 0.34008794566089406 1105 94-95 -0.07871114269342172 1105 96-97 -0.043004463333105036 1105 98-99 0.09845973922191575 1105 100-101 0.32869837821349535 1106 1 -0.08275746794795324 1106 2 -0.16977013404869012 1106 3 -0.10331938501693827 1106 4 -0.014018901362327085 1106 5 -0.050811846059573895 1106 6 -0.2714667025334023 1106 7 -0.09884163442865201 1106 8 -0.22523148808306104 1106 9 -0.1404275063649294 1106 10-11 -0.2081728718486886 1106 12-13 -0.10365590192881768 1106 14-15 -0.06777721325680375 1106 16-17 -0.20084710992466626 1106 18-19 -0.10656062097697117 1106 20-21 -0.17319773460175725 1106 22-23 -0.12101839723444385 1106 24-25 -0.20734561274061747 1106 26-27 -0.2108511405020863 1106 28-29 -0.06018875068342311 1106 30-31 0.13560051310600585 1106 32-33 0.04971590445607177 1106 34-35 -0.10838597162699415 1106 36-37 0.008001990611461451 1106 38-39 0.0707547249213647 1106 40-41 0.018834358255809036 1106 42-43 -0.06296335444837808 1106 44-45 0.159336265571703 1106 46-47 0.15714381745723927 1106 48-49 0.28899949744396736 1106 50-51 0.4299671731097803 1106 52-53 0.5712833473170704 1106 54-55 0.48118825595370396 1106 56-57 0.5671820321574401 1106 58-59 0.3778198626273266 1106 60-61 0.24330835253389438 1106 62-63 0.08439571766309228 1106 64-65 0.2969002776001659 1106 66-67 0.29772501261462736 1106 68-69 0.2667119691723343 1106 70-71 0.012940433009493546 1106 72-73 0.25655095149456386 1106 74-75 0.44806389073923825 1106 76-77 0.4756194828593294 1106 78-79 0.40986592323666926 1106 80-81 0.49339630138527824 1106 82-83 0.6261362301310989 1106 84-85 0.4905664642201728 1106 86-87 0.49159310671326395 1106 88-89 0.7463625442690116 1106 90-91 0.8372499043918893 1106 92-93 0.8328565407022204 1106 94-95 0.8102351382983137 1106 96-97 0.49160297468342407 1106 98-99 0.6940175904615842 1106 100-101 1.117954576560603 1107 1 -0.09855912084051255 1107 2 -0.04880319189992832 1107 3 -0.08784831063677956 1107 4 -0.07034947987472151 1107 5 -0.1066134989521359 1107 6 -0.08748323145902503 1107 7 -0.05526312203195971 1107 8 0.007074297040901456 1107 9 -0.14912998570377312 1107 10-11 -0.15397452474125117 1107 12-13 -0.08031705895361085 1107 14-15 -0.12085985788490206 1107 16-17 -0.03811157273457866 1107 18-19 -0.0451556622992868 1107 20-21 0.05135185217510241 1107 22-23 -0.11495641376337318 1107 24-25 -0.1408621416662328 1107 26-27 -0.1983635371963004 1107 28-29 -0.2541267672123553 1107 30-31 -0.2972920488774733 1107 32-33 -0.31954029389103056 1107 34-35 -0.33541489724682805 1107 36-37 -0.10409718294226167 1107 38-39 -0.19055106020260126 1107 40-41 -0.14518630290121592 1107 42-43 -0.34309971808473705 1107 44-45 -0.27656042864317243 1107 46-47 -0.4606123808898701 1107 48-49 -0.7517732298287569 1107 50-51 -0.5702063806092283 1107 52-53 -0.7952951650796294 1107 54-55 -0.6536795126413324 1107 56-57 -0.6131898686690036 1107 58-59 -0.7238619555544901 1107 60-61 -0.9164602425074335 1107 62-63 -0.695550563328645 1107 64-65 -0.5687898050444673 1107 66-67 -0.4162336650713243 1107 68-69 -0.4036268738028781 1107 70-71 -0.6657951041805852 1107 72-73 -0.5171102055302299 1107 74-75 -0.5321179274425809 1107 76-77 -0.4398391948266216 1107 78-79 -0.37234068833358336 1107 80-81 -0.19723179778827316 1107 82-83 -0.1538389764804684 1107 84-85 -0.23392527131701968 1107 86-87 -0.3218035875016163 1107 88-89 -0.23597629870619485 1107 90-91 -0.20408893858331467 1107 92-93 -0.3646227981407577 1107 94-95 -0.24690948980085992 1107 96-97 -0.2797358682917839 1107 98-99 -0.21687910375328912 1107 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2316 66-67 -0.7902731722930767 2316 68-69 -0.6700397879914917 2316 70-71 -0.5991222069843261 2316 72-73 -0.6082053627094979 2316 74-75 -0.6687585391673032 2316 76-77 -0.6169984981401306 2316 78-79 -0.7601155396504886 2316 80-81 -0.815792120642989 2316 82-83 -0.9116669289018802 2316 84-85 -1.2193759934920436 2316 86-87 -1.2120737659213283 2316 88-89 -1.3510200540163062 2316 90-91 -1.7399011730777936 2316 92-93 -1.9784800623718546 2316 94-95 -2.268904816903664 2316 96-97 -2.0312256728797173 2316 98-99 -2.4595749406266982 2316 100-101 -3.407311894436539 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 2.0 4 14.0 5 41.0 6 130.0 7 272.0 8 602.0 9 1074.0 10 1754.0 11 2826.0 12 3805.0 13 5123.0 14 5825.0 15 7429.0 16 9625.0 17 12885.0 18 12212.0 19 7865.0 20 7048.0 21 7286.0 22 7556.0 23 8276.0 24 9207.0 25 10787.0 26 12367.0 27 14435.0 28 17647.0 29 21038.0 30 25516.0 31 30799.0 32 37717.0 33 46865.0 34 61573.0 35 85426.0 36 124536.0 37 200346.0 38 183315.0 39 20223.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.368430529879234 20.530019287252006 15.359375125047018 21.74217505782174 2 15.980335880540375 22.63586100780107 38.17601639141683 23.207786720241725 3 19.144313828663453 26.034130182609633 32.0208639775495 22.800692011177414 4 13.147714090958013 18.563917357282662 37.14597210429597 31.142396447463355 5 12.820842451856192 38.060515101868354 34.54744222942403 14.571200216851429 6 29.410275728186303 37.082989520176355 17.861242989201276 15.64549176243607 7 25.422641043119153 34.766087466067134 22.021980124609847 17.789291366203862 8 25.11480369763576 33.710132622188205 21.148296081925196 20.02676759825084 9 25.231600515022144 17.366351355122116 22.238133961564678 35.16391416829106 10-11 23.984953939002562 27.06816070923173 28.053060834001027 20.893824517764674 12-13 25.571427432503 25.29448344315423 28.163579324172954 20.970509800169815 14-15 22.43052590090483 26.647579175633652 27.805634463916718 23.116260459544797 16-17 20.701215531940974 29.558693240877034 28.433152719325435 21.30693850785656 18-19 20.287776738586768 27.927906829577715 31.21994436203444 20.56437206980108 20-21 21.515329084002026 26.726041704037662 31.04558065557695 20.713048556383367 22-23 21.116057369958902 27.086646894918744 30.935610273336444 20.861685461785914 24-25 21.36006505542866 27.219388670310092 31.014288675492203 20.40625759876905 26-27 21.013482471989164 27.126237354888282 31.439452449596445 20.42082772352611 28-29 20.396889935941132 27.943987355648304 31.11608726675516 20.543035441655405 30-31 21.067873699987643 27.244302667192848 30.85374287957969 20.834080753239817 32-33 20.72356226306789 27.59110550380088 30.75353878411723 20.931793449014002 34-35 21.374884951192396 27.429429671853644 30.69076126307271 20.504924113881252 36-37 21.132003255340386 27.053762454841625 30.937096477584152 20.87713781223384 38-39 20.84989898647605 27.35772142991695 30.98135111942121 20.811028464185796 40-41 20.940749326527012 27.265664787353955 30.871224390336273 20.92236149578276 42-43 20.76572004323264 27.385755651860634 30.243215998269896 21.60530830663683 44-45 20.84486043897344 27.299856891149577 30.177979175469787 21.677303494407195 46-47 21.414210067104396 27.57299540130342 29.656834288965968 21.355960242626214 48-49 21.23056209963207 27.58946118000662 29.466631189135107 21.713345531226206 50-51 20.54764282283252 28.032001722065765 29.644345585113697 21.776009869988023 52-53 21.020786245879226 28.23772337889605 28.948719021936277 21.792771353288448 54-55 20.426089140287726 27.69300375105137 29.303643821528087 22.57726328713282 56-57 20.977037267353097 27.98962979793752 28.748161347030056 22.28517158767933 58-59 21.182452313197935 27.670876980731947 28.900088046372087 22.24658265969803 60-61 20.788554219268836 27.951335600192934 29.27652165940501 21.983588521133218 62-63 20.467745343075702 27.93711109250866 29.180668332914777 22.41447523150086 64-65 20.176550547509994 28.25331954094466 28.813186878894058 22.75694303265129 66-67 20.43618929074519 28.001510802085427 28.419921260901866 23.142378646267517 68-69 20.47860121880123 27.79330207435385 28.505398900786787 23.22269780605813 70-71 20.647197523703603 27.79908793204137 28.21226906802042 23.34144547623461 72-73 20.547021681156647 27.561856471460516 28.419346416171376 23.47177543121146 74-75 20.19483742122194 28.241410650434702 27.911250363744355 23.652501564599003 76-77 20.286287022216946 27.819688754263904 28.131373412585397 23.762650810933753 78-79 20.23906189639488 28.005632609318727 27.956384662354978 23.79892083193141 80-81 20.433374208917922 27.827204403999595 27.793919287578923 23.945502099503564 82-83 20.284926433950005 27.642577821360664 27.957291429983695 24.115204314705636 84-85 20.100214061061216 27.7481633401498 27.721206395522657 24.430416203266326 86-87 20.140784836037966 27.658391673474192 27.711088677180452 24.48973481330739 88-89 20.049916971670395 27.594197914344353 27.365957485096388 24.98992762888886 90-91 20.108538433367322 27.38282180941023 27.397860507828366 25.11077924939408 92-93 20.235098440184483 27.432602655632753 27.416757353615317 24.91554155056744 94-95 19.96982415193958 27.099565848473993 27.598194830542383 25.332415169044044 96-97 19.762878543268634 27.099602173297775 27.647012512805798 25.490506770627793 98-99 19.75731749731676 27.250508495183123 27.149257910949316 25.842916096550805 100-101 19.800616576817728 26.893641744652292 27.60160387704938 25.704137801480602 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1605.0 1 1103.5 2 678.5 3 752.0 4 673.5 5 680.0 6 1010.5 7 1664.0 8 2376.0 9 2648.5 10 2311.0 11 2022.5 12 2033.0 13 2078.0 14 2181.0 15 2341.0 16 2494.5 17 2608.0 18 2720.0 19 2890.5 20 3207.5 21 3543.5 22 3988.5 23 4588.5 24 5131.0 25 5908.5 26 7197.5 27 8576.5 28 9849.5 29 11340.5 30 13121.5 31 14962.0 32 16624.0 33 18067.0 34 19700.5 35 21390.5 36 23275.0 37 25342.0 38 26605.0 39 27028.5 40 27506.5 41 28929.0 42 30632.0 43 32275.5 44 34070.5 45 35294.5 46 36338.5 47 39361.5 48 44461.0 49 45823.5 50 42348.5 51 38329.5 52 34329.5 53 31715.5 54 29969.5 55 27146.0 56 24312.0 57 21366.0 58 18983.5 59 16668.5 60 13789.5 61 11075.0 62 8675.5 63 6636.0 64 4856.5 65 3614.5 66 2579.5 67 1901.0 68 1405.5 69 969.5 70 675.0 71 484.0 72 374.0 73 300.5 74 248.5 75 192.5 76 117.5 77 65.5 78 44.0 79 24.0 80 16.0 81 8.0 82 4.0 83 5.0 84 5.5 85 3.5 86 1.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.381483120268832 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.491399688276071E-4 16-17 0.002292087713213985 18-19 4.982799376552142E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.965598753104284E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0073247150835316485 36-37 0.017688937786760103 38-39 0.012058374491256183 40-41 0.007075575114704041 42-43 0.004335035457600363 44-45 0.001993119750620857 46-47 0.001544667806731164 48-49 0.0 50-51 5.97935925186257E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.4879595635864993E-4 60-61 7.972479002483427E-4 62-63 0.0 64-65 0.0 66-67 0.0011460438566069926 68-69 6.975919127172999E-4 70-71 0.0017938077755587712 72-73 0.0 74-75 0.0 76-77 0.09930719157468419 78-79 0.03632460745506511 80-81 4.982799376552142E-5 82-83 0.0 84-85 0.0 86-87 0.14923484132773665 88-89 0.3180022562115577 90-91 0.2691209943275812 92-93 0.0 94-95 4.982799376552142E-5 96-97 0.0 98-99 9.965598753104284E-5 100-101 8.470758940138641E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1003452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.435499065516844 #Duplication Level Percentage of deduplicated Percentage of total 1 81.41212802384217 50.01594715130494 2 11.236726775628034 13.806678346471285 3 3.1719533599777985 5.846116130483373 4 1.372333789429105 3.372400449521958 5 0.7464813630301387 2.2930227540431907 6 0.43639296261907734 1.608601166830947 7 0.2823022572741546 1.2140366044071735 8 0.21375955046963577 1.0505939730498086 9 0.15637082967187224 0.8646047964162361 >10 0.8733651209655521 9.890891044207875 >50 0.06476116072219966 2.6858863308157415 >100 0.02904388579727868 3.123367088167287 >500 0.0019470758102261303 0.8247769635549032 >1k 0.002109332127744974 2.2340949126411034 >5k 3.2451263503768834E-4 1.1689822880841612 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6258 0.6236471699692661 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5469 0.5450185958072733 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3644 0.3631464185631201 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3308 0.3296620067526897 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2107 0.20997516572790728 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2028 0.2021023427129549 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2011 0.20040819092492715 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1392 0.13872113464321162 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1259 0.12546688830158292 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1185 0.11809234522428577 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1156 0.11520232158588552 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1124 0.11201332998489215 RNA PCR Primer, Index 17 (95% over 21bp) ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1100 0.10962158628414712 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1075 0.10713018659587105 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1023 0.10194807524425682 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.965598753104284E-5 0.0 12-13 4.982799376552142E-5 0.0 0.0 9.965598753104284E-5 0.0 14-15 9.965598753104284E-5 0.0 0.0 1.9931197506208568E-4 0.0 16-17 9.965598753104284E-5 0.0 0.0 1.9931197506208568E-4 0.0 18-19 9.965598753104284E-5 0.0 0.0 2.491399688276071E-4 0.0 20-21 9.965598753104284E-5 0.0 0.0 7.474199064828213E-4 0.0 22-23 9.965598753104284E-5 0.0 0.0 0.001893463763089814 0.0 24-25 9.965598753104284E-5 0.0 0.0 0.004235379470069321 0.0 26-27 9.965598753104284E-5 0.0 0.0 0.008271446965076555 0.0 28-29 1.9931197506208568E-4 0.0 0.0 0.026458664689491877 0.0 30-31 2.989679625931285E-4 0.0 0.0 0.07279869889142679 0.0 32-33 2.989679625931285E-4 0.0 0.0 0.16343581955091024 0.0 34-35 2.989679625931285E-4 0.0 0.0 0.3228853996005788 0.0 36-37 2.989679625931285E-4 0.0 0.0 0.5659961811825578 0.0 38-39 2.989679625931285E-4 0.0 0.0 0.8962561238604339 0.0 40-41 2.989679625931285E-4 0.0 0.0 1.2390727209672212 0.0 42-43 2.989679625931285E-4 0.0 0.0 1.5881178172946986 0.0 44-45 2.989679625931285E-4 0.0 0.0 2.013947852014845 0.0 46-47 2.989679625931285E-4 0.0 0.0 2.5147690173521005 0.0 48-49 3.9862395012417136E-4 0.0 0.0 3.001588516441245 0.0 50-51 3.9862395012417136E-4 0.0 0.0 3.491547179137617 0.0 52-53 3.9862395012417136E-4 0.0 0.0 3.9741811267504574 0.0 54-55 3.9862395012417136E-4 0.0 0.0 4.514117267193647 0.0 56-57 3.9862395012417136E-4 0.0 0.0 5.151516963442198 0.0 58-59 3.9862395012417136E-4 0.0 0.0 5.796590170730638 0.0 60-61 3.9862395012417136E-4 0.0 0.0 6.483120268831992 0.0 62-63 3.9862395012417136E-4 0.0 0.0 7.160232876111662 0.0 64-65 3.9862395012417136E-4 0.0 0.0 7.883536033611971 0.0 66-67 4.982799376552142E-4 0.0 0.0 8.70420309093011 0.0 68-69 4.982799376552142E-4 0.0 0.0 9.593832091619728 0.0 70-71 4.982799376552142E-4 0.0 0.0 10.527658522779365 0.0 72-73 4.982799376552142E-4 0.0 0.0 11.443546876183415 0.0 74-75 4.982799376552142E-4 0.0 0.0 12.394663621179689 0.0 76-77 4.982799376552142E-4 0.0 0.0 13.366658295563713 0.0 78-79 4.982799376552142E-4 0.0 0.0 14.461229834610922 0.0 80-81 5.97935925186257E-4 0.0 0.0 15.608120767111929 0.0 82-83 5.97935925186257E-4 0.0 0.0 16.744647476909705 0.0 84-85 6.975919127172999E-4 0.0 0.0 17.892534969286025 0.0 86-87 6.975919127172999E-4 0.0 0.0 19.053278084053847 0.0 88-89 6.975919127172999E-4 0.0 0.0 20.278996902691908 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTCGA 35 0.008433477 27.116123 92-93 AGTACTT 5565 0.0 21.489317 12-13 GAGTACT 5515 0.0 21.469023 12-13 GTACATG 4000 0.0 21.082085 1 TACATGG 4155 0.0 20.214725 2 AGAGTAC 8490 0.0 20.094057 10-11 GTATCAA 11560 0.0 20.071112 1 GTACTTT 6010 0.0 19.779736 14-15 ACATGGG 4030 0.0 19.664234 3 GGTATCA 9190 0.0 19.233515 1 CATGGGA 2245 0.0 19.023558 4 ACGGGGC 150 7.771458E-4 18.981285 8 TAGGGTG 285 4.3190084E-8 18.315275 5 TAAGGTG 260 3.313162E-7 18.251236 5 TACTTTT 6735 0.0 17.96759 14-15 CATGGGG 1470 0.0 17.754602 4 CAACGCA 13235 0.0 17.425207 5 ATCAACG 13210 0.0 17.38634 3 TCAACGC 13260 0.0 17.32078 4 TATCAAC 13380 0.0 17.271833 2 >>END_MODULE