FastQCFastQC Report
Thu 26 May 2016
SRR938800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003199
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66550.6633778542442726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54680.5450563646893588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.3463918923364158No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29960.2986446358100437No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24070.23993245607302238No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19820.197567980031878No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15140.1509172158265708No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12240.12200969099849582No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.11911893851568832No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11540.1150320125917191No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11200.11164285450842755No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10340.10307027818010185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG39650.022.2944951
GAGTACT53800.022.18913812-13
GTATCAA108300.021.7222391
TACATGG40650.021.3578832
ACCGTGC1353.8266342E-421.0855628
CATTCCG1805.2243868E-621.0845099
GGTATCA84450.020.822361
ACATGGG39200.020.211483
AGTACTT55000.020.15157112-13
GTACTTT59300.020.09110314-15
TATCAAC121550.019.047262
GTCCTAC1507.692137E-419.010251
AGAGTAC81100.018.87387310-11
ATCAACG123000.018.784153
CAACGCA123350.018.730855
TCAACGC123600.018.654584
TAGGCAT2803.5355697E-818.638135
AACGCAG124900.018.4984026
GCCGTAT1303.273144E-718.27451594-95
AGCGTAC650.0075994118.25487310-11
Produced by FastQC (version 0.11.5)