##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938800_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1003199 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.477824439617663 33.0 31.0 34.0 30.0 34.0 2 31.51972739207276 33.0 31.0 34.0 28.0 34.0 3 31.51528061730524 33.0 31.0 34.0 28.0 34.0 4 35.08864841372449 37.0 35.0 37.0 32.0 37.0 5 35.12506292370706 37.0 35.0 37.0 32.0 37.0 6 35.19454265803694 37.0 35.0 37.0 32.0 37.0 7 35.11464026578974 37.0 35.0 37.0 32.0 37.0 8 35.09171061773387 37.0 35.0 37.0 32.0 37.0 9 36.63561765910851 39.0 37.0 39.0 32.0 39.0 10-11 36.53492228361472 39.0 37.0 39.0 32.0 39.0 12-13 36.430808344107206 39.0 37.0 39.0 32.0 39.0 14-15 37.53538480401197 40.0 37.0 41.0 32.0 41.0 16-17 37.44343146275066 40.0 37.0 41.0 32.0 41.0 18-19 37.421563917029424 40.0 37.0 41.0 32.0 41.0 20-21 37.32443662722949 40.0 37.0 41.0 31.5 41.0 22-23 37.29444207978676 40.0 37.0 41.0 31.5 41.0 24-25 37.20951027662508 40.0 37.0 41.0 31.0 41.0 26-27 37.06233110280213 40.0 36.5 41.0 31.0 41.0 28-29 36.883685091392636 40.0 36.0 41.0 30.5 41.0 30-31 36.61691199851675 39.0 36.0 41.0 30.0 41.0 32-33 36.37972127165199 39.0 35.0 41.0 30.0 41.0 34-35 36.04179280481739 39.0 35.0 41.0 29.5 41.0 36-37 35.795192180215494 39.0 35.0 41.0 28.0 41.0 38-39 35.60707446877439 38.5 35.0 40.0 27.0 41.0 40-41 35.464786149109 38.5 35.0 40.5 27.0 41.0 42-43 35.382758555381336 39.0 35.0 41.0 26.0 41.0 44-45 35.13693743713859 39.0 35.0 41.0 24.5 41.0 46-47 34.73430246640996 39.0 34.5 41.0 23.5 41.0 48-49 34.35279640430264 38.5 34.0 41.0 21.0 41.0 50-51 33.162062561864595 37.5 32.5 39.5 17.0 40.5 52-53 33.21809082744301 37.5 33.0 39.5 17.0 40.5 54-55 33.69952222839137 38.0 33.0 40.0 16.5 41.0 56-57 33.58653318035604 38.0 33.0 40.0 14.0 41.0 58-59 33.378007254791925 38.0 33.0 40.0 12.5 41.0 60-61 33.05752198716307 37.5 33.0 40.0 8.5 41.0 62-63 32.66356076909965 37.0 32.5 40.0 7.5 41.0 64-65 32.26788354055377 36.0 32.0 39.5 3.5 41.0 66-67 31.86257113493933 36.0 31.0 39.0 2.0 41.0 68-69 31.423314317498324 35.0 31.0 39.0 2.0 40.5 70-71 30.975553703701856 35.0 31.0 38.0 2.0 40.0 72-73 30.52055275174716 35.0 30.0 37.5 2.0 40.0 74-75 30.034410421063022 35.0 30.0 37.0 2.0 39.0 76-77 29.540843342148467 34.5 29.0 36.0 2.0 39.0 78-79 29.061040232296882 34.0 29.0 36.0 2.0 38.5 80-81 28.550171002961527 34.0 28.5 35.0 2.0 37.0 82-83 28.101260069039142 34.0 27.5 35.0 2.0 37.0 84-85 27.700352572121783 34.0 26.5 35.0 2.0 36.5 86-87 27.2258041525161 34.0 26.0 35.0 2.0 36.0 88-89 26.72339286622096 34.0 25.0 35.0 2.0 35.5 90-91 26.20847359297607 33.0 24.0 35.0 2.0 35.0 92-93 25.694159384130167 33.0 21.5 35.0 2.0 35.0 94-95 25.12399035485482 33.0 18.0 35.0 2.0 35.0 96-97 24.483873588390736 32.0 4.5 35.0 2.0 35.0 98-99 23.695576351252342 32.0 2.0 35.0 2.0 35.0 100-101 21.379223862862702 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27709757265084534 1101 2 0.3677261068887212 1101 3 0.34909385153320116 1101 4 0.10745119419033244 1101 5 0.25340000807788243 1101 6 0.4126890140998114 1101 7 0.48760769234510803 1101 8 0.4168437647239571 1101 9 0.7899073834183952 1101 10-11 0.5051730031524215 1101 12-13 0.5726177429797161 1101 14-15 0.5022422038337524 1101 16-17 0.6612943729475873 1101 18-19 0.7370684867136532 1101 20-21 0.7843039185205285 1101 22-23 0.6600461202947727 1101 24-25 0.578266479394479 1101 26-27 0.5058395682629637 1101 28-29 0.684680829609313 1101 30-31 0.7202089934300773 1101 32-33 0.5999276988150228 1101 34-35 0.4689948895251277 1101 36-37 0.7268916895988582 1101 38-39 0.7193098055527898 1101 40-41 0.743788168330525 1101 42-43 0.7462077109981493 1101 44-45 0.7155097514382334 1101 46-47 0.6182099142199533 1101 48-49 0.5337168729928266 1101 50-51 0.6882429479307106 1101 52-53 0.7761331213577876 1101 54-55 0.8223134564664463 1101 56-57 0.8185332828579988 1101 58-59 0.7977252649153002 1101 60-61 0.7187052342835685 1101 62-63 0.9192717111896442 1101 64-65 0.7809662412237728 1101 66-67 0.6560939624171205 1101 68-69 0.9499907801081129 1101 70-71 0.6101849988748889 1101 72-73 0.49072391196639487 1101 74-75 0.5397128634806307 1101 76-77 0.5789135419610822 1101 78-79 0.5571018276040078 1101 80-81 0.5943833736657105 1101 82-83 0.49466105331078225 1101 84-85 0.6124491469780224 1101 86-87 0.5468368571621092 1101 88-89 0.5699607121376218 1101 90-91 0.3098102142763324 1101 92-93 0.504804677384513 1101 94-95 0.32415988786000227 1101 96-97 -0.0832273686172762 1101 98-99 0.1262463857877627 1101 100-101 0.1078224028355308 1102 1 0.26070915344768864 1102 2 0.4543153360333889 1102 3 0.4902659519800565 1102 4 0.4440806558680208 1102 5 0.656764770551618 1102 6 0.5744921370261835 1102 7 0.6243101237128101 1102 8 0.7154496247852862 1102 9 0.7522861071322211 1102 10-11 0.8149640585234152 1102 12-13 0.8677217192766946 1102 14-15 0.862004269735742 1102 16-17 0.9671172880756842 1102 18-19 0.9773790598233347 1102 20-21 0.9376471869012875 1102 22-23 0.8437621954513475 1102 24-25 0.6706381706819826 1102 26-27 0.7802375683202811 1102 28-29 0.8270389630843269 1102 30-31 0.7767935710325702 1102 32-33 0.9369853889605935 1102 34-35 0.9930219786958432 1102 36-37 1.0022177935685406 1102 38-39 0.9396011111370726 1102 40-41 1.1046084049639546 1102 42-43 1.1369829974207164 1102 44-45 1.1074225713970947 1102 46-47 0.9631559364900397 1102 48-49 1.0039607813464215 1102 50-51 1.1359830920623892 1102 52-53 1.0041220678826832 1102 54-55 0.8652333022380532 1102 56-57 0.9794964827662866 1102 58-59 0.7415182117664756 1102 60-61 0.7482579587425207 1102 62-63 0.6929659174844431 1102 64-65 0.7422118404460107 1102 66-67 0.8860493428846148 1102 68-69 0.8484521337289301 1102 70-71 0.8195071523405062 1102 72-73 0.6404126334095608 1102 74-75 0.7732131158984394 1102 76-77 0.6981197816411644 1102 78-79 0.7041274860397202 1102 80-81 0.6212460297183355 1102 82-83 0.5764582019815627 1102 84-85 0.5529358614240323 1102 86-87 0.28776745218474176 1102 88-89 0.37804932511829037 1102 90-91 0.5197253842560308 1102 92-93 0.4697290732902708 1102 94-95 0.0975912048637042 1102 96-97 0.16313627175458123 1102 98-99 -0.3301266678945609 1102 100-101 -0.20496570068002384 1103 1 0.1256416919662975 1103 2 0.09687731950446477 1103 3 0.2389417400371343 1103 4 0.28535422856596426 1103 5 0.20305180103868992 1103 6 0.16232793474525664 1103 7 0.17620456633604675 1103 8 0.15203842031315418 1103 9 0.1260395362804161 1103 10-11 0.007884916371196482 1103 12-13 0.21047478609978754 1103 14-15 0.17102443916585486 1103 16-17 0.14796279955793779 1103 18-19 0.17419922580273095 1103 20-21 0.1623523664247486 1103 22-23 -0.0026604912754706334 1103 24-25 0.07134815532630512 1103 26-27 -0.12448171600139801 1103 28-29 0.05282141274701502 1103 30-31 0.24376914859602294 1103 32-33 0.1276255397568491 1103 34-35 -0.07895993955671088 1103 36-37 0.022441141107321982 1103 38-39 -0.10151847339682973 1103 40-41 -0.05312378503241888 1103 42-43 -0.02401982353892862 1103 44-45 0.15230559215314088 1103 46-47 0.0880750603534679 1103 48-49 -0.12646654659229029 1103 50-51 -0.19266086314733144 1103 52-53 -0.2312220978206554 1103 54-55 -0.018270553936133638 1103 56-57 -0.018923736699200333 1103 58-59 -0.043679082872841946 1103 60-61 -0.16619881242329626 1103 62-63 -0.05023617364894761 1103 64-65 -0.36226826618108277 1103 66-67 -0.3406432329017193 1103 68-69 -0.34299893513008683 1103 70-71 -0.30335340425984825 1103 72-73 -0.15456743871038725 1103 74-75 -0.21699655818150987 1103 76-77 -0.14002981548784277 1103 78-79 -0.422907357556614 1103 80-81 -0.32232183722208774 1103 82-83 -0.49157156906348476 1103 84-85 -0.5277450940789947 1103 86-87 -0.606662230867073 1103 88-89 -0.6329293408352292 1103 90-91 -0.6068339076572187 1103 92-93 -0.6852921486079673 1103 94-95 -0.5034705370573072 1103 96-97 -0.28163611044662673 1103 98-99 -0.14294154565297745 1103 100-101 0.2599006272414357 1104 1 0.06443091456926808 1104 2 -0.01519685908856161 1104 3 -0.0582429673471303 1104 4 -0.005800108597242115 1104 5 0.10360948430147232 1104 6 0.26480223428163896 1104 7 0.07736696638979623 1104 8 -0.04145415510846817 1104 9 0.22897577225434418 1104 10-11 0.25220309818938347 1104 12-13 0.13209257149252096 1104 14-15 0.2407054510586164 1104 16-17 0.2719465393953371 1104 18-19 0.3598556794747054 1104 20-21 0.27223864607266535 1104 22-23 0.15852173005087167 1104 24-25 0.14300289563404078 1104 26-27 0.00808564273004464 1104 28-29 0.2067638302703685 1104 30-31 0.013464438524131594 1104 32-33 -0.1036618351003753 1104 34-35 0.13553814550678567 1104 36-37 0.2441874288181296 1104 38-39 -0.10774621645938254 1104 40-41 0.028815340143957258 1104 42-43 -0.07410633163541291 1104 44-45 0.02059491554224735 1104 46-47 0.07880273622526346 1104 48-49 0.0968881198056124 1104 50-51 0.12436611394062425 1104 52-53 0.1537011367422707 1104 54-55 0.15008847986081264 1104 56-57 0.17865907590579155 1104 58-59 0.23990789555892889 1104 60-61 0.15851730663872132 1104 62-63 0.3543571543091204 1104 64-65 0.4468302018697088 1104 66-67 0.26497223440997786 1104 68-69 0.24363094459846124 1104 70-71 0.20780446041797163 1104 72-73 0.13654490423122922 1104 74-75 0.13890446983703342 1104 76-77 -0.08577748060912072 1104 78-79 -0.07099578058960176 1104 80-81 0.03956756679592033 1104 82-83 -0.08494152135805066 1104 84-85 0.15785676710490648 1104 86-87 0.2767912059400217 1104 88-89 0.5142982006550625 1104 90-91 0.5200054392486493 1104 92-93 0.2338983707765685 1104 94-95 0.2631885706494721 1104 96-97 0.1671981299618892 1104 98-99 0.01906796183878967 1104 100-101 0.36035500471909643 1105 1 0.0012694253085001606 1105 2 0.09516040645592483 1105 3 0.16228344314836107 1105 4 0.16205867009114883 1105 5 0.2588784967501496 1105 6 0.17941184315439074 1105 7 0.15352717970219487 1105 8 0.19817058520332154 1105 9 0.23705064425055156 1105 10-11 0.3069063175052804 1105 12-13 0.3651320686379975 1105 14-15 0.4291808154400272 1105 16-17 0.48926058627825597 1105 18-19 0.5657884304521161 1105 20-21 0.6325693293593773 1105 22-23 0.5036074885040236 1105 24-25 0.5697268572901493 1105 26-27 0.4315798600665772 1105 28-29 0.7024984002921215 1105 30-31 0.6081984932590458 1105 32-33 0.7755596319130831 1105 34-35 0.5494177646852663 1105 36-37 0.5723126844167581 1105 38-39 0.7274345837892682 1105 40-41 0.6088688318257525 1105 42-43 0.44778518003624157 1105 44-45 0.2915356003012093 1105 46-47 0.34114201580518255 1105 48-49 0.6309093201785885 1105 50-51 0.6484997368160847 1105 52-53 0.6071126628092429 1105 54-55 0.4382370869505081 1105 56-57 0.4538478099359864 1105 58-59 0.37717662991629197 1105 60-61 0.5163676607961847 1105 62-63 0.4695145621709358 1105 64-65 0.32089164369137535 1105 66-67 0.44683577336730096 1105 68-69 0.607537963950346 1105 70-71 0.5238889756393803 1105 72-73 0.39962133026711655 1105 74-75 0.29415610157870375 1105 76-77 0.24270356828587225 1105 78-79 0.19588702028065796 1105 80-81 0.3337759062636181 1105 82-83 0.22216228031377128 1105 84-85 0.36595699448545105 1105 86-87 0.5240767484634539 1105 88-89 0.6969039980410017 1105 90-91 0.7287456856048351 1105 92-93 0.6476788176856623 1105 94-95 0.5906697176943432 1105 96-97 0.39649361833930996 1105 98-99 0.26323622344092357 1105 100-101 0.6288030916144507 1106 1 0.08559122289125298 1106 2 0.025324150920752686 1106 3 0.04386480201655729 1106 4 0.1271587711874389 1106 5 0.05576264197409131 1106 6 0.036546817545300314 1106 7 0.2296706221393805 1106 8 0.09957607987556827 1106 9 -0.07092847433954574 1106 10-11 0.16889016968791282 1106 12-13 0.04915688354112291 1106 14-15 0.2591429585238032 1106 16-17 0.3917189210075023 1106 18-19 0.29156916776531716 1106 20-21 0.4559547939589237 1106 22-23 0.4112704102066189 1106 24-25 0.3445752621167628 1106 26-27 0.42936579211331605 1106 28-29 0.04164394939477489 1106 30-31 -0.036767778416056274 1106 32-33 0.12082465988700619 1106 34-35 0.17835544561906147 1106 36-37 0.06630208605676557 1106 38-39 0.32592464265371746 1106 40-41 0.11247913221772166 1106 42-43 0.10137124096597461 1106 44-45 0.07682963680228738 1106 46-47 0.03833398249267361 1106 48-49 -0.20592953863541652 1106 50-51 -0.19736881435381548 1106 52-53 -0.16259963645155295 1106 54-55 -0.36665691959611735 1106 56-57 -0.2713515744210824 1106 58-59 -0.22899812675301234 1106 60-61 -0.2778176272297159 1106 62-63 -0.35178788522647864 1106 64-65 -0.3028532743969805 1106 66-67 -0.2584286239864575 1106 68-69 -0.16079879509560513 1106 70-71 -0.06600001550631873 1106 72-73 -0.121444831640666 1106 74-75 -0.26468568567764805 1106 76-77 -0.2919110735317325 1106 78-79 -0.066890427954295 1106 80-81 -0.0793458211626401 1106 82-83 -0.202849100409356 1106 84-85 -0.13156437269904586 1106 86-87 -0.18866739514452036 1106 88-89 -0.05495978012259073 1106 90-91 -0.05976451369084401 1106 92-93 2.565761069064365E-4 1106 94-95 -0.10286415941749638 1106 96-97 -0.15537488947545341 1106 98-99 -0.03332965567313195 1106 100-101 0.3477677118530238 1107 1 0.07579690359644431 1107 2 0.01846812741438697 1107 3 0.07324775990098331 1107 4 0.2544849220199552 1107 5 0.15664413071061745 1107 6 -0.09175876521669579 1107 7 0.03378423624319993 1107 8 0.12700017390103113 1107 9 0.3678570300679027 1107 10-11 0.2467550080816494 1107 12-13 0.31115492467726824 1107 14-15 0.32868262551694727 1107 16-17 0.46566603168330545 1107 18-19 0.3885329287839312 1107 20-21 0.2076687998355098 1107 22-23 0.3990275110881143 1107 24-25 0.39648515437924914 1107 26-27 0.19577127693212049 1107 28-29 0.15183004146137336 1107 30-31 0.4196475007220428 1107 32-33 0.4592183915226542 1107 34-35 0.6279147012507948 1107 36-37 0.6906899411008425 1107 38-39 0.31808918721787194 1107 40-41 0.5532235004841297 1107 42-43 0.41576889032933906 1107 44-45 0.842255689691612 1107 46-47 0.9555719844515309 1107 48-49 1.0947560637152236 1107 50-51 0.8883314794757737 1107 52-53 0.8119351333819438 1107 54-55 0.7219816700219042 1107 56-57 0.8339373579981171 1107 58-59 0.9193858105633481 1107 60-61 0.9724272307526505 1107 62-63 0.9611406162426661 1107 64-65 0.8744239048390554 1107 66-67 0.6127760772867994 1107 68-69 0.7490934673921537 1107 70-71 0.8615220217120978 1107 72-73 0.8633739734523793 1107 74-75 0.7181742555564377 1107 76-77 0.5802730596710113 1107 78-79 0.8003965456010427 1107 80-81 0.6978334148804528 1107 82-83 0.2849080004721323 1107 84-85 0.5765649123156464 1107 86-87 0.2843982267947567 1107 88-89 0.46560829039650997 1107 90-91 0.5166311768086658 1107 92-93 0.6363976143047516 1107 94-95 0.5513963694757464 1107 96-97 0.5517749636097591 1107 98-99 0.6679436058351946 1107 100-101 0.919266242705131 1108 1 0.06371898900081518 1108 2 0.29174849111360146 1108 3 0.06422127333805605 1108 4 0.1713411026544378 1108 5 0.21061135378540996 1108 6 0.11710430810452266 1108 7 0.09475099317124602 1108 8 0.07831455059998405 1108 9 0.1348266048268414 1108 10-11 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-1.5754926799116475 2316 92-93 -1.2298575447676647 2316 94-95 -1.0833612008183913 2316 96-97 -1.6359381196841873 2316 98-99 -2.1994128050584294 2316 100-101 -1.9788736487614678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6373.0 3 1323.0 4 1046.0 5 1198.0 6 1435.0 7 1700.0 8 2239.0 9 2589.0 10 3159.0 11 4164.0 12 5680.0 13 7195.0 14 7917.0 15 8941.0 16 10257.0 17 13299.0 18 11869.0 19 8311.0 20 8295.0 21 8833.0 22 9531.0 23 10448.0 24 11755.0 25 12997.0 26 15474.0 27 17483.0 28 20122.0 29 23607.0 30 27773.0 31 32663.0 32 39295.0 33 48471.0 34 62304.0 35 85042.0 36 118739.0 37 172505.0 38 155082.0 39 24082.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98492708427039 19.636670764740572 14.851419832678642 21.526982318310395 2 15.819395755973996 22.185135225535646 38.657020275593105 23.338448742897253 3 19.316380676728 25.8764215852242 31.7216624270044 23.085535311043394 4 13.04385592691511 17.91039113396401 37.83481017397599 31.210942765144896 5 13.021449973873175 37.58026630378854 34.48611081970292 14.912172902635362 6 29.412933832709115 36.543820224719106 18.22721598002497 15.816029962546816 7 25.431950983605244 34.636813454728575 22.475240006075094 17.455995555591084 8 25.00272296594444 33.10517043768305 21.59476870777529 20.297337888597216 9 25.525328264652238 17.22988858019361 22.568675271311868 34.676107883842285 10-11 23.98920850195049 26.77917632422838 28.230794624427602 21.000820549393527 12-13 25.605423652964525 25.222493535837952 28.315452106764933 20.85663070443259 14-15 22.558345627791216 26.431212196102223 28.109499309762924 22.90094286634364 16-17 20.62355429647593 29.249443710277518 28.734088514541504 21.39291347870505 18-19 20.30720627920523 27.870917012941472 31.41620815853075 20.405668549322552 20-21 21.575460301622474 26.69252515809486 31.213266634605734 20.518747905676932 22-23 21.173510554480565 26.764879716741934 31.20535060779879 20.856259120978713 24-25 21.366910787614778 26.83905002647696 31.291076763216 20.50296242269226 26-27 20.890005070826547 26.703152613876064 31.802233764103548 20.604608551193845 28-29 20.47884972077932 27.69554196809335 31.235370286119956 20.590238025007373 30-31 20.93173901590902 26.987952650311968 31.077793700070327 21.00251463370869 32-33 20.741964366415004 27.212317900835387 30.95050358318323 21.095214149566377 34-35 21.447402445081202 27.128267481460327 30.730318336708578 20.694011736749893 36-37 21.237006627089258 26.79445516019844 31.031089029152685 20.937449183559615 38-39 20.86983665487922 27.322251564392335 30.919115826312805 20.88879595441564 40-41 21.057369089041554 27.21648752474099 30.842448785278727 20.883694600938732 42-43 20.73918632978005 27.392213457122992 30.159722673816585 21.708877539280373 44-45 20.97824189204941 27.136577364023708 30.21091752449932 21.674263219427566 46-47 21.314879862159295 27.463079633125194 29.868287882555233 21.353752622160275 48-49 21.16275881256211 27.581320360638774 29.579364849028302 21.676555977770818 50-51 20.55431608115052 27.87981829344138 29.813314973879308 21.75255065152879 52-53 21.09174002539745 28.090698309738794 29.055511136150535 21.762050528713225 54-55 20.56697350798223 27.588501430642005 29.459017697534414 22.385507363841356 56-57 20.964840670532354 27.724537898523753 29.061070626793285 22.24955080415061 58-59 21.42426695966109 27.314695113931254 29.168081545002135 22.092956381405525 60-61 20.917763348657886 27.33137826979877 29.853180261123963 21.897678120419382 62-63 20.68420057370234 27.269571063950703 29.828419577117142 22.217808785229817 64-65 20.536885816057833 27.60208416945987 29.44079523515465 22.42023477932765 66-67 20.65731764197192 27.437879464241032 29.00397955772461 22.90082333606244 68-69 20.59557162404147 27.198998655732353 29.32380234561191 22.88162737461426 70-71 20.844243588973637 27.14601725622085 29.148950013012232 22.860789141793287 72-73 20.776507408117276 26.85508194292995 29.39391406914243 22.974496579810346 74-75 20.471837706083722 27.63016794944353 28.92885755046915 22.9691367940036 76-77 20.672494094938283 27.01839037098447 29.06761314428464 23.24150238979261 78-79 20.711098661437806 27.11493827407511 29.13858820704696 23.035374857440125 80-81 20.9621697443999 26.889486748755115 28.986854308843203 23.16148919800178 82-83 20.87189837041247 26.696077823160834 29.21250277668267 23.219521029744026 84-85 20.82431118119817 26.698682799141878 29.025797684992476 23.451208334667477 86-87 20.9610825310888 26.490153691758998 29.165613989096844 23.383149788055356 88-89 20.983510922256627 26.38807795200621 28.838349801397595 23.790061324339565 90-91 21.04656062309025 26.18458308020125 29.041464286107477 23.727392010601022 92-93 21.528443958039027 26.070880078545468 29.010230026727278 23.390445936688227 94-95 21.34211951326685 25.684562327526926 29.046342144536563 23.926976014669656 96-97 21.34705275906593 25.535498013152232 29.15851742120172 23.958931806580118 98-99 21.540954547661073 25.48589908032367 28.728962973231447 24.244183398783807 100-101 21.92319449732506 24.807866167484764 29.285844010492628 23.983095324697544 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2362.0 1 1598.0 2 889.5 3 927.0 4 846.0 5 885.5 6 1204.0 7 1875.5 8 2620.0 9 2916.5 10 2598.0 11 2204.0 12 2180.5 13 2260.0 14 2410.0 15 2523.0 16 2560.0 17 2771.5 18 2948.5 19 3106.0 20 3360.5 21 3760.5 22 4253.5 23 4701.5 24 5303.5 25 6143.0 26 7239.5 27 8478.0 28 9704.0 29 11100.0 30 12882.0 31 14885.0 32 16706.0 33 18142.5 34 19803.5 35 21522.5 36 23417.0 37 25610.5 38 26762.0 39 27026.5 40 27544.0 41 28903.0 42 30428.0 43 31827.5 44 33351.0 45 34580.5 46 36153.5 47 39516.0 48 44803.5 49 45858.5 50 40592.0 51 35790.5 52 33283.5 53 31453.5 54 29544.5 55 27031.5 56 24123.5 57 20929.0 58 18549.5 59 16582.5 60 13900.5 61 11158.5 62 8823.0 63 6777.0 64 5104.0 65 3893.0 66 2832.0 67 2098.5 68 1628.5 69 1193.0 70 858.0 71 630.0 72 479.0 73 394.0 74 347.0 75 283.5 76 201.5 77 128.5 78 84.0 79 56.0 80 39.5 81 29.0 82 24.0 83 24.0 84 19.0 85 10.0 86 6.5 87 5.5 88 5.5 89 5.0 90 2.0 91 2.0 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 0.0 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2720297767441953 2 0.164473848159737 3 0.193381372987812 4 0.16188213903721993 5 0.2304627496638254 6 0.1942785030686833 7 0.23833755815147345 8 0.24441810647737885 9 0.14872423118444097 10-11 0.20339932555754142 12-13 0.20957955500354367 14-15 0.17259785944762704 16-17 0.2812004397931019 18-19 0.2300640251834382 20-21 0.1974184583517328 22-23 0.2407797455938453 24-25 0.2321573287054712 26-27 0.33512792576547623 28-29 0.3084133855795311 30-31 0.284539757316345 32-33 0.19183631562631143 34-35 0.35072802106062706 36-37 0.3879090788567373 38-39 0.3677734925971816 40-41 0.33293494112334643 42-43 0.3642348128337448 44-45 0.37205978076134444 46-47 0.3772431990063786 48-49 0.3959334090245305 50-51 0.38830780333712456 52-53 0.3873608326962048 54-55 0.3810310815700574 56-57 0.3623408715519054 58-59 0.3396634167298811 60-61 0.3051239086163363 62-63 0.3714118534807152 64-65 0.40350917415188814 66-67 0.40799482455624464 68-69 0.41287919944098833 70-71 0.4125801560806978 72-73 0.40455582591290457 74-75 0.39867463982719276 76-77 0.38322406621218724 78-79 0.3607459736303565 80-81 0.3696175933189726 82-83 0.3792866619683632 84-85 0.3794860242085568 86-87 0.3751498954843456 88-89 0.3828253417318 90-91 0.4037085363920817 92-93 0.40196411679038757 94-95 0.41168302599982654 96-97 0.4115833448797297 98-99 0.4084433895966802 100-101 0.3818285305308319 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1003199.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.15519412395466 #Duplication Level Percentage of deduplicated Percentage of total 1 82.38614707737575 52.03113111796346 2 10.65762841176478 13.461691824919583 3 3.077666006967198 5.831117823561294 4 1.2722603024144779 3.2139938550075047 5 0.6720547310994447 2.1221873502253787 6 0.40343788785619167 1.5287518874709622 7 0.2695916987636807 1.1918281248738873 8 0.19290528845488097 0.9746376751924382 9 0.1495912213218403 0.850271635963826 >10 0.8291098762066845 9.52684632301314 >50 0.05900570828868229 2.4973182812858563 >100 0.0269549447918018 2.9295348399942354 >500 0.001744143114812038 0.8141211787061585 >1k 0.0015855846498291255 1.812596243020932 >5k 3.171169299658251E-4 1.2139718388013234 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6655 0.6633778542442726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5468 0.5450563646893588 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3475 0.3463918923364158 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2996 0.2986446358100437 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2407 0.23993245607302238 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1982 0.197567980031878 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1514 0.1509172158265708 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1224 0.12200969099849582 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1195 0.11911893851568832 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1154 0.1150320125917191 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1120 0.11164285450842755 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1034 0.10307027818010185 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.96811200968103E-5 0.0 5 0.0 0.0 0.0 9.96811200968103E-5 0.0 6 0.0 0.0 0.0 9.96811200968103E-5 0.0 7 0.0 0.0 0.0 9.96811200968103E-5 0.0 8 0.0 0.0 0.0 9.96811200968103E-5 0.0 9 0.0 0.0 0.0 9.96811200968103E-5 0.0 10-11 0.0 0.0 0.0 1.993622401936206E-4 0.0 12-13 0.0 0.0 0.0 2.9904336029043093E-4 0.0 14-15 0.0 0.0 0.0 2.9904336029043093E-4 4.984056004840515E-5 16-17 0.0 0.0 0.0 3.488839203388361E-4 9.96811200968103E-5 18-19 4.984056004840515E-5 0.0 0.0 3.987244803872412E-4 9.96811200968103E-5 20-21 9.96811200968103E-5 0.0 0.0 4.984056004840515E-4 9.96811200968103E-5 22-23 1.993622401936206E-4 0.0 0.0 0.0015450573615005598 9.96811200968103E-5 24-25 1.993622401936206E-4 0.0 0.0 0.003787882563678792 9.96811200968103E-5 26-27 1.993622401936206E-4 0.0 0.0 0.008871619688616116 9.96811200968103E-5 28-29 2.9904336029043093E-4 0.0 0.0 0.025169482824444603 9.96811200968103E-5 30-31 2.9904336029043093E-4 0.0 0.0 0.06628794486437885 9.96811200968103E-5 32-33 2.9904336029043093E-4 0.0 0.0 0.1558015907113145 9.96811200968103E-5 34-35 2.9904336029043093E-4 0.0 0.0 0.3086127478197247 9.96811200968103E-5 36-37 3.987244803872412E-4 0.0 0.0 0.5344901659590968 9.96811200968103E-5 38-39 4.984056004840515E-4 0.0 0.0 0.841557856417321 9.96811200968103E-5 40-41 4.984056004840515E-4 0.0 0.0 1.1720506100982955 9.96811200968103E-5 42-43 4.984056004840515E-4 0.0 0.0 1.5074277386640138 9.96811200968103E-5 44-45 4.984056004840515E-4 0.0 0.0 1.9327670781171036 9.96811200968103E-5 46-47 4.984056004840515E-4 0.0 0.0 2.4153732210658108 9.96811200968103E-5 48-49 4.984056004840515E-4 0.0 0.0 2.8808342113578664 9.96811200968103E-5 50-51 4.984056004840515E-4 0.0 0.0 3.373009741835867 9.96811200968103E-5 52-53 4.984056004840515E-4 0.0 0.0 3.843155744772473 9.96811200968103E-5 54-55 4.984056004840515E-4 0.0 0.0 4.357211281111724 9.96811200968103E-5 56-57 4.984056004840515E-4 0.0 0.0 4.984753772681193 9.96811200968103E-5 58-59 4.984056004840515E-4 0.0 0.0 5.606165875364709 9.96811200968103E-5 60-61 4.984056004840515E-4 0.0 0.0 6.281106739540211 9.96811200968103E-5 62-63 4.984056004840515E-4 0.0 0.0 6.9511133882709215 9.96811200968103E-5 64-65 4.984056004840515E-4 0.0 0.0 7.63367985813383 9.96811200968103E-5 66-67 4.984056004840515E-4 0.0 0.0 8.412637971130355 9.96811200968103E-5 68-69 4.984056004840515E-4 0.0 0.0 9.2846982502973 9.96811200968103E-5 70-71 4.984056004840515E-4 0.0 0.0 10.195135760701517 9.96811200968103E-5 72-73 5.482461605324567E-4 0.0 0.0 11.094957231815423 9.96811200968103E-5 74-75 5.980867205808619E-4 0.0 0.0 12.024084952237791 9.96811200968103E-5 76-77 5.980867205808619E-4 0.0 0.0 12.99507874310082 9.96811200968103E-5 78-79 5.980867205808619E-4 0.0 0.0 14.083447052877844 9.96811200968103E-5 80-81 6.977678406776721E-4 0.0 0.0 15.21422967925606 9.96811200968103E-5 82-83 6.977678406776721E-4 0.0 0.0 16.342420596511758 9.96811200968103E-5 84-85 6.977678406776721E-4 0.0 0.0 17.477489511054138 9.96811200968103E-5 86-87 6.977678406776721E-4 0.0 0.0 18.630052462173506 9.96811200968103E-5 88-89 6.977678406776721E-4 0.0 0.0 19.810974691960418 9.96811200968103E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 3965 0.0 22.294495 1 GAGTACT 5380 0.0 22.189138 12-13 GTATCAA 10830 0.0 21.722239 1 TACATGG 4065 0.0 21.357883 2 ACCGTGC 135 3.8266342E-4 21.085562 8 CATTCCG 180 5.2243868E-6 21.084509 9 GGTATCA 8445 0.0 20.82236 1 ACATGGG 3920 0.0 20.21148 3 AGTACTT 5500 0.0 20.151571 12-13 GTACTTT 5930 0.0 20.091103 14-15 TATCAAC 12155 0.0 19.04726 2 GTCCTAC 150 7.692137E-4 19.01025 1 AGAGTAC 8110 0.0 18.873873 10-11 ATCAACG 12300 0.0 18.78415 3 CAACGCA 12335 0.0 18.73085 5 TCAACGC 12360 0.0 18.65458 4 TAGGCAT 280 3.5355697E-8 18.63813 5 AACGCAG 12490 0.0 18.498402 6 GCCGTAT 130 3.273144E-7 18.274515 94-95 AGCGTAC 65 0.00759941 18.254873 10-11 >>END_MODULE