FastQCFastQC Report
Thu 26 May 2016
SRR938800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003199
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62550.6235054062055484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55680.5550244766990398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36310.36194214707151817No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34440.3433017776134147No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22530.2245815635781136No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT19920.19856479123284612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19830.19766766115197482No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.1384570758144695No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12770.12729279036362676No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11930.1189195762754947No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11270.1123406223491052No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11150.11114444890794348No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.11064604330745943No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT10920.10885178314571685RNA PCR Primer, Index 17 (95% over 21bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10070.10037888793748798No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC700.008337934527.1782251
GAGTACT56700.022.08892312-13
AGTACTT58200.020.70448312-13
GGTATCA94100.020.5714041
GTACATG41350.020.359021
GTACTTT62950.019.85813914-15
AGAGTAC84950.019.57394410-11
GTACTAC1951.02088725E-519.5125711
TACATGG41000.019.328342
GTATCAA119200.019.3120421
ACATGGG41150.018.2200343
TACTCCG1901.8796093E-417.4826135
TACTTTT69750.017.17397714-15
TATCAAC134650.017.0569322
ACTTTTT73700.017.02554516-17
TCAACGC135350.016.863544
ATCAACG135650.016.8612273
CAACGCA135700.016.8200455
AGTACAT34500.016.7804152
GAGTACA40050.016.7446351
Produced by FastQC (version 0.11.5)