##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938800_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1003199 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00269936473222 31.0 31.0 34.0 30.0 34.0 2 32.19976594873001 33.0 31.0 34.0 30.0 34.0 3 32.2648278158172 34.0 31.0 34.0 30.0 34.0 4 35.91037471129856 37.0 35.0 37.0 35.0 37.0 5 35.81123386287267 37.0 35.0 37.0 35.0 37.0 6 35.844119661203806 37.0 35.0 37.0 35.0 37.0 7 35.76427309038386 37.0 35.0 37.0 33.0 37.0 8 35.759373763331105 37.0 35.0 37.0 33.0 37.0 9 37.379348464262826 39.0 37.0 39.0 34.0 39.0 10-11 37.328392970886135 39.0 37.0 39.0 34.0 39.0 12-13 37.254715166183374 39.0 37.0 39.0 33.0 39.0 14-15 38.43798588315977 40.0 38.0 41.0 33.0 41.0 16-17 38.38221379806001 40.0 38.0 41.0 33.0 41.0 18-19 38.521462840373644 40.0 38.0 41.0 34.0 41.0 20-21 38.52089166755549 40.0 38.0 41.0 34.0 41.0 22-23 38.435253125252316 40.0 38.0 41.0 34.0 41.0 24-25 38.340079585406286 40.0 38.0 41.0 33.5 41.0 26-27 38.14477835404541 40.0 37.5 41.0 33.0 41.0 28-29 37.95961867984318 40.0 37.0 41.0 33.0 41.0 30-31 37.696140546392094 40.0 37.0 41.0 33.0 41.0 32-33 37.4679704624905 40.0 36.0 41.0 32.0 41.0 34-35 37.358886920740545 40.0 36.0 41.0 32.0 41.0 36-37 37.19751764106623 40.0 36.0 41.0 31.0 41.0 38-39 37.0355253543913 40.0 36.0 41.0 31.0 41.0 40-41 36.8315483767428 40.0 35.0 41.0 31.0 41.0 42-43 36.57943588460515 39.0 35.0 41.0 30.0 41.0 44-45 36.25812475889629 39.0 35.0 41.0 30.0 41.0 46-47 35.84006712526627 39.0 35.0 41.0 28.5 41.0 48-49 35.445516791783085 39.0 35.0 41.0 27.0 41.0 50-51 35.16170321142664 39.0 35.0 40.5 26.0 41.0 52-53 34.93421694000891 38.5 35.0 40.0 25.5 41.0 54-55 34.650776167041634 38.0 34.0 40.0 24.0 41.0 56-57 34.35377128565719 38.0 34.0 40.0 23.5 41.0 58-59 34.01416717919376 38.0 34.0 40.0 22.5 41.0 60-61 33.62209292473378 37.0 33.0 40.0 21.5 41.0 62-63 33.254168913645245 37.0 33.0 40.0 20.5 41.0 64-65 32.834570708304135 36.0 32.5 39.0 18.5 41.0 66-67 32.39990221282118 36.0 32.0 39.0 15.5 40.0 68-69 32.0645574806195 35.0 32.0 39.0 12.5 40.0 70-71 31.632108883681106 35.0 31.0 38.0 8.0 40.0 72-73 31.1623745637705 35.0 31.0 37.0 6.5 39.5 74-75 30.671645904750704 35.0 30.5 37.0 2.0 39.0 76-77 28.72486714998719 32.5 28.0 35.0 2.0 37.0 78-79 29.630362968862606 34.0 29.5 36.0 2.0 37.5 80-81 29.660505044363084 34.0 30.0 35.0 2.0 37.0 82-83 29.33758157653666 34.0 29.5 35.0 2.0 37.0 84-85 28.9541790811195 34.0 29.0 35.0 2.0 36.0 86-87 28.40482795537077 34.0 29.0 35.0 2.0 36.0 88-89 27.81886146218248 34.0 27.5 35.0 2.0 35.5 90-91 27.31130712849594 33.0 26.0 35.0 2.0 35.0 92-93 27.022861864894203 33.0 26.0 35.0 2.0 35.0 94-95 26.704728573294034 33.0 25.0 35.0 2.0 35.0 96-97 26.142171194349274 33.0 24.5 35.0 2.0 35.0 98-99 25.38726015476491 33.0 21.0 35.0 2.0 35.0 100-101 22.146197314789987 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11140002279555006 1101 2 0.21221670600721865 1101 3 0.10151416150299752 1101 4 0.1234901935365329 1101 5 0.14838778277871967 1101 6 0.07504723742889752 1101 7 0.19454921862974572 1101 8 0.2139596689648755 1101 9 0.3039636885014403 1101 10-11 0.3801011456010386 1101 12-13 0.2671963976502738 1101 14-15 0.3612310712021838 1101 16-17 0.3799194118667302 1101 18-19 0.2995599356587846 1101 20-21 0.30610295146596656 1101 22-23 0.37056294754683705 1101 24-25 0.15223762062655055 1101 26-27 0.17025964988360442 1101 28-29 0.17324199856783906 1101 30-31 0.28576939474351093 1101 32-33 0.25338855643351366 1101 34-35 0.4748550300887828 1101 36-37 0.5358110494729829 1101 38-39 0.41505110597251615 1101 40-41 0.4321220985476515 1101 42-43 0.343197540988335 1101 44-45 0.413348129208849 1101 46-47 0.3469421384938727 1101 48-49 0.10374693186355444 1101 50-51 0.4058022427081198 1101 52-53 0.27168773447069583 1101 54-55 0.2167072858095267 1101 56-57 0.27173654141944326 1101 58-59 0.23476119687963148 1101 60-61 0.4245873196091381 1101 62-63 0.4271424582269603 1101 64-65 0.41104931588531457 1101 66-67 0.6197356881777623 1101 68-69 0.4132545160822545 1101 70-71 0.6307465508169692 1101 72-73 0.4227628059550028 1101 74-75 0.43573584519925035 1101 76-77 0.4811390021880193 1101 78-79 0.4330058240878536 1101 80-81 0.7235078851791812 1101 82-83 0.8103529741218196 1101 84-85 0.6126571757788497 1101 86-87 0.6904291505157012 1101 88-89 0.6645544879533176 1101 90-91 0.5007025298364525 1101 92-93 0.3438453846556584 1101 94-95 0.5891894729224738 1101 96-97 0.798137193692579 1101 98-99 0.4887108190566991 1101 100-101 0.962487000781767 1102 1 0.41372711659577277 1102 2 0.3503366452505787 1102 3 0.30589322471326597 1102 4 0.23049660649207482 1102 5 0.06722278089385014 1102 6 0.07890625381511285 1102 7 0.2398345168906033 1102 8 0.19537664775516106 1102 9 0.37943220461539795 1102 10-11 0.4432987391444314 1102 12-13 0.5542351289035636 1102 14-15 0.5249846452840572 1102 16-17 0.5446738390987562 1102 18-19 0.5090485305623673 1102 20-21 0.4428869501608901 1102 22-23 0.4438605602695773 1102 24-25 0.45262749040541195 1102 26-27 0.373042087776696 1102 28-29 0.6057461375585689 1102 30-31 0.5284443180812488 1102 32-33 0.5201721362691956 1102 34-35 0.6089627386421128 1102 36-37 0.6476448152601222 1102 38-39 0.7293625344368735 1102 40-41 0.7657060125889075 1102 42-43 0.7944764946338481 1102 44-45 0.6736311237438315 1102 46-47 0.6897679613869556 1102 48-49 0.6491369449363731 1102 50-51 0.4197860791501924 1102 52-53 0.39823834038366357 1102 54-55 0.45039326923592427 1102 56-57 0.4237146593305283 1102 58-59 0.6015474221421329 1102 60-61 0.42157208415513026 1102 62-63 0.5951733568123245 1102 64-65 0.7098585431340965 1102 66-67 0.5281576249388351 1102 68-69 0.6812125552037074 1102 70-71 0.622557301390696 1102 72-73 0.44913330239139526 1102 74-75 0.4472136908518216 1102 76-77 0.5733580303022912 1102 78-79 0.4615488773072265 1102 80-81 0.5185925719055327 1102 82-83 0.6286115998666695 1102 84-85 0.6338751523359605 1102 86-87 0.6243717403444755 1102 88-89 0.568297326011308 1102 90-91 0.750039519737534 1102 92-93 0.9093637891105573 1102 94-95 0.8808080993727749 1102 96-97 0.8843940910919059 1102 98-99 1.1996147173135796 1102 100-101 1.8165817908340784 1103 1 0.03844890268593559 1103 2 0.016001166722659832 1103 3 0.04260150125357853 1103 4 -0.1274719574928156 1103 5 -0.07840386897933627 1103 6 -0.11985925283614307 1103 7 -0.04126505349535847 1103 8 -0.06147733523280863 1103 9 0.03877544725243354 1103 10-11 0.06533867254562864 1103 12-13 -0.07938087551334405 1103 14-15 0.03796796107625511 1103 16-17 -0.15008344622362557 1103 18-19 -0.22809863381245066 1103 20-21 -0.16348565806216442 1103 22-23 -0.019641656814911812 1103 24-25 0.030923139053598447 1103 26-27 -0.04629960203851624 1103 28-29 -0.15751323544647988 1103 30-31 -0.14969885209077205 1103 32-33 0.14535437716361344 1103 34-35 0.19883877169996111 1103 36-37 0.09627984174436222 1103 38-39 0.016508404793498244 1103 40-41 0.13507487678601393 1103 42-43 -0.14221474400036271 1103 44-45 0.09402743838004568 1103 46-47 0.06604149095095835 1103 48-49 0.14099864889952585 1103 50-51 0.043605287556140127 1103 52-53 0.11024380976719073 1103 54-55 0.05830144570549578 1103 56-57 -0.03470637967981105 1103 58-59 -0.14122440794465518 1103 60-61 -0.058570128443427905 1103 62-63 -0.25604258894242804 1103 64-65 -0.11135451264672014 1103 66-67 -0.18594307555633804 1103 68-69 -0.23970568010055615 1103 70-71 -0.4373179983901849 1103 72-73 -0.34982156225430217 1103 74-75 -0.40865640697595396 1103 76-77 -0.4295707641903661 1103 78-79 -0.5331165327240015 1103 80-81 -0.39909600967800074 1103 82-83 -0.4944247275371829 1103 84-85 -0.43924334522265696 1103 86-87 -0.48457488571877505 1103 88-89 -0.7325155330787361 1103 90-91 -0.753837143075831 1103 92-93 -0.7352211761234884 1103 94-95 -0.4287684978770052 1103 96-97 -0.3420472294967851 1103 98-99 -0.3593860418437558 1103 100-101 0.05144069971794707 1104 1 0.04675535971745148 1104 2 0.12507695789378914 1104 3 -0.0266805655340292 1104 4 0.02832660462503611 1104 5 -0.15159139837528812 1104 6 -0.06179804205874717 1104 7 -0.12121365256813021 1104 8 -0.1989832144238335 1104 9 -0.02927198942028042 1104 10-11 7.683574209877975E-4 1104 12-13 -0.1253225539383962 1104 14-15 -0.2639620933482547 1104 16-17 -0.09228259962012686 1104 18-19 -0.00360592400931381 1104 20-21 -0.0778636396518948 1104 22-23 -0.022950801476604 1104 24-25 0.016808881175485624 1104 26-27 0.12243318033062423 1104 28-29 -0.010789624615334503 1104 30-31 -0.1126641480660382 1104 32-33 0.032528502004041115 1104 34-35 -0.15882181420341368 1104 36-37 -0.001923645455804035 1104 38-39 0.03992018333276803 1104 40-41 0.13585596930768418 1104 42-43 0.055691424110911214 1104 44-45 0.05590088459383935 1104 46-47 0.12117570448317849 1104 48-49 0.007284847918541004 1104 50-51 0.19115618455271743 1104 52-53 -0.2138801907703325 1104 54-55 -0.07198142737116342 1104 56-57 -0.11841346158534094 1104 58-59 -0.050798250583902416 1104 60-61 0.08618395339490803 1104 62-63 0.08466492970300266 1104 64-65 -0.1909839556353674 1104 66-67 -0.22321294722200236 1104 68-69 0.04333957287263246 1104 70-71 -0.16625789626023746 1104 72-73 -0.0743520668568678 1104 74-75 -0.13343047806377228 1104 76-77 -0.015230577400068057 1104 78-79 -0.2660668787563871 1104 80-81 -0.03022716603088682 1104 82-83 -0.20650814485896163 1104 84-85 -0.29023524873673523 1104 86-87 -0.029080361765274176 1104 88-89 -0.08229800301087309 1104 90-91 -0.13589217230380868 1104 92-93 -0.08595271546367655 1104 94-95 -0.43000732741405656 1104 96-97 -0.2498615142512044 1104 98-99 0.10846318210302996 1104 100-101 1.0519404309549252 1105 1 0.11959279080805629 1105 2 0.027104979683066688 1105 3 0.03514976604895992 1105 4 0.10529655440502239 1105 5 -0.058813184375843264 1105 6 0.03478347959148209 1105 7 0.04227336711092278 1105 8 -0.019597959806702647 1105 9 0.18232891683022956 1105 10-11 0.05565765254130639 1105 12-13 0.02402883897190833 1105 14-15 0.059938628053330945 1105 16-17 0.17818471647154865 1105 18-19 0.07090925650466318 1105 20-21 0.022477226684330276 1105 22-23 -0.16128282614494083 1105 24-25 0.08587163166213685 1105 26-27 -0.03140652456541915 1105 28-29 -0.05824406671508342 1105 30-31 -0.03120690869485543 1105 32-33 -0.026271452192688116 1105 34-35 0.03652813099623842 1105 36-37 0.09191376927649486 1105 38-39 0.03675280507951584 1105 40-41 -0.09820081039492123 1105 42-43 -0.12220522407086776 1105 44-45 -0.13814244039675572 1105 46-47 -0.06703485971501522 1105 48-49 -0.05102435690389484 1105 50-51 -0.06222078457915359 1105 52-53 -0.0998535512584624 1105 54-55 -0.17089605333835323 1105 56-57 -0.00456062914353339 1105 58-59 0.16904111078758177 1105 60-61 0.22257854042931058 1105 62-63 0.015377252419789045 1105 64-65 -0.06666384799766689 1105 66-67 -0.019224790640230083 1105 68-69 -0.26005089186702435 1105 70-71 -0.016430067499054957 1105 72-73 -0.06394306814917528 1105 74-75 0.04597011247130922 1105 76-77 -0.03429414580235246 1105 78-79 -0.45475899077811377 1105 80-81 -0.2443462300082473 1105 82-83 -0.08301515439585572 1105 84-85 -0.005564827837353903 1105 86-87 0.08115523883017062 1105 88-89 -0.18710775583677375 1105 90-91 -0.04926570619005588 1105 92-93 0.23044056290572712 1105 94-95 0.1660067652721402 1105 96-97 0.2105483023721142 1105 98-99 0.11580601961590986 1105 100-101 0.4513265852792472 1106 1 0.095512954296332 1106 2 -0.002759811414158264 1106 3 -0.028052168378366105 1106 4 0.08109847661408054 1106 5 0.01581282831144648 1106 6 0.05809179175633972 1106 7 0.12263802321601247 1106 8 0.10164664789829203 1106 9 0.12099815064645014 1106 10-11 0.13178877817857426 1106 12-13 0.14332049488190535 1106 14-15 0.09690183727929735 1106 16-17 0.2870755738587647 1106 18-19 0.08542632956003615 1106 20-21 0.05865314605954808 1106 22-23 0.1497646536407231 1106 24-25 0.16102923743389397 1106 26-27 0.3106881591096027 1106 28-29 0.16327702851446446 1106 30-31 0.14069866776093676 1106 32-33 0.09750943095143327 1106 34-35 0.01493727341195239 1106 36-37 0.09872791566314021 1106 38-39 0.2360524225689744 1106 40-41 0.1416698533524965 1106 42-43 0.11125626967120894 1106 44-45 0.16510505633683437 1106 46-47 0.13459312563094272 1106 48-49 0.09656046643378602 1106 50-51 -0.05494549720357611 1106 52-53 -0.13095445639545744 1106 54-55 -0.3235859439060462 1106 56-57 -0.18645660834883415 1106 58-59 -0.05709090165928643 1106 60-61 -0.21789830694287815 1106 62-63 -0.3015507603187402 1106 64-65 -0.09512255321624963 1106 66-67 -0.4174072251099261 1106 68-69 -0.4677372647566642 1106 70-71 -0.4686704441369436 1106 72-73 -0.2215291330202831 1106 74-75 -0.03157005507658184 1106 76-77 0.0909770701318422 1106 78-79 -0.24405106053258407 1106 80-81 -0.3027904309632419 1106 82-83 -0.23292095551008174 1106 84-85 -0.19135094512876094 1106 86-87 -0.04903678500727082 1106 88-89 0.011953408406274235 1106 90-91 -0.10440851209502355 1106 92-93 -0.13886045377220313 1106 94-95 -0.0075581304686132 1106 96-97 0.1047371376363948 1106 98-99 0.2533265394045756 1106 100-101 0.9527361198618642 1107 1 0.07004772412982874 1107 2 0.09616281346341538 1107 3 0.031693179319965736 1107 4 -0.15102689067288821 1107 5 0.055969537420160975 1107 6 -0.012427307166284152 1107 7 0.06681040323559984 1107 8 0.044839595988399594 1107 9 -0.05334073280115348 1107 10-11 -0.04206038930428946 1107 12-13 0.06496594052342886 1107 14-15 0.10277842885618327 1107 16-17 0.08090515270303911 1107 18-19 0.150558552870514 1107 20-21 0.004294673973355145 1107 22-23 0.07385867910596033 1107 24-25 -0.041713171968659424 1107 26-27 0.10745603374231649 1107 28-29 0.10206253292191292 1107 30-31 -0.031191635862960254 1107 32-33 -0.07193229284877134 1107 34-35 -0.1456895630229198 1107 36-37 -0.21175200598230504 1107 38-39 -0.2717830328668782 1107 40-41 -0.3470666794584716 1107 42-43 -0.30696116421713526 1107 44-45 -0.16349435600401563 1107 46-47 -0.21604350512321702 1107 48-49 -0.2859077020285099 1107 50-51 -0.3801168977912326 1107 52-53 -0.3689564152593121 1107 54-55 -0.19968780482670923 1107 56-57 -0.23787678464658057 1107 58-59 -0.1334865921587962 1107 60-61 -0.06314806208489898 1107 62-63 -0.12819130880061635 1107 64-65 -0.16123420845620728 1107 66-67 0.004238220531597392 1107 68-69 -0.23806047729339497 1107 70-71 -0.1802277409048152 1107 72-73 -0.30576027895564195 1107 74-75 -0.39347015301977706 1107 76-77 -0.43350872810518126 1107 78-79 -0.26168083526323827 1107 80-81 -0.2856503721973276 1107 82-83 -0.30735778215062837 1107 84-85 -0.32210510771446366 1107 86-87 -0.3639241503353823 1107 88-89 -0.4145891968826554 1107 90-91 -0.2464261418697582 1107 92-93 -0.060995615378473644 1107 94-95 -0.1128470628878091 1107 96-97 -0.03825992406781964 1107 98-99 0.2454910839687301 1107 100-101 0.7842738279911465 1108 1 0.11834681226299182 1108 2 0.029443072410550997 1108 3 0.010163484724650118 1108 4 0.029941751792556204 1108 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pass #Quality Count 2 1.0 3 7.0 4 11.0 5 40.0 6 123.0 7 315.0 8 643.0 9 1036.0 10 1798.0 11 2764.0 12 3773.0 13 5233.0 14 5987.0 15 7643.0 16 9567.0 17 12929.0 18 12354.0 19 8286.0 20 7302.0 21 7593.0 22 8011.0 23 8616.0 24 9837.0 25 11315.0 26 12698.0 27 14780.0 28 18207.0 29 21721.0 30 26239.0 31 31793.0 32 38565.0 33 48079.0 34 63122.0 35 87127.0 36 127318.0 37 199829.0 38 172085.0 39 16451.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.364219486851574 20.617556550235793 15.322216449524417 21.696007513388217 2 15.986658678886242 22.67007841913718 38.1604248010614 23.182838100915173 3 19.148543808357065 25.920081658773586 32.16450574611817 22.766868786751182 4 13.110559320732976 18.68313265862506 37.1481630264783 31.058144994163673 5 12.77116987046862 38.136327514674605 34.506537391205505 14.585965223651268 6 29.40895592614117 37.14463438923319 17.826122929910273 15.620286754715362 7 25.436229501823664 34.77565268705411 22.066509236950992 17.721608574171228 8 25.08575068356328 33.7822306441693 21.107477180499583 20.024541491767835 9 25.23975801411285 17.304443086566078 22.228690419348503 35.22710847997257 10-11 23.970518311920166 27.119046171298017 28.021010786494006 20.889424730287807 12-13 25.518865150383924 25.28959857416126 28.19585147114381 20.99568480431101 14-15 22.4241152552983 26.661759032853897 27.810434420289493 23.103691291558302 16-17 20.63229877437909 29.60435222520722 28.406642974984177 21.356706025429514 18-19 20.27633600113238 27.965039837559647 31.191568173413252 20.567055987894726 20-21 21.533314925553153 26.666344364378354 31.011145346038028 20.789195364030466 22-23 21.058085185491613 27.137985584116414 30.920535207870024 20.883394022521955 24-25 21.4009240476677 27.264589636112618 30.95534766421284 20.379138652006837 26-27 21.04523146041431 27.062320041826805 31.449100339967373 20.44334815779151 28-29 20.389523913002307 28.05839120653031 31.002174045229314 20.549910835238073 30-31 21.093920548166416 27.1975948939343 30.824243245856504 20.884241312042775 32-33 20.667434875832214 27.629812230674073 30.72939666008439 20.973356233409323 34-35 21.425267441385202 27.474561790897774 30.600817252623575 20.499353515093453 36-37 21.233251612401506 27.14981051976706 30.858707865448597 20.758230002382835 38-39 20.857507313880497 27.423973776205475 30.89231585196676 20.826203057947268 40-41 21.011089162408602 27.317162406607938 30.780488218232243 20.891260212751217 42-43 20.79491939053422 27.461998109874507 30.162704314989014 21.580378184602257 44-45 20.92176859389618 27.273207573877905 30.141846976325276 21.663176855900637 46-47 21.447197650703654 27.52533408293909 29.671130409472145 21.356337856885112 48-49 21.260032466739535 27.600317191173474 29.446246213916638 21.693404128170354 50-51 20.475259881269757 27.95005175944741 29.787474761973826 21.78721359730901 52-53 21.089634260002253 28.261092764247174 28.861521991150312 21.78775098460026 54-55 20.467773592278302 27.805749407644942 29.211003998209726 22.51547300186703 56-57 20.969921285665798 27.984981825377087 28.70543753417243 22.33965935478469 58-59 21.20255025702931 27.75732087444789 28.81748576794296 22.222643100579848 60-61 20.789833857171196 28.034745751729762 29.210415352087825 21.965005039011217 62-63 20.460650984011984 28.040179866406028 29.176356598806407 22.322812550775577 64-65 20.24252815414942 28.305717055470964 28.758924722072006 22.69283006830761 66-67 20.44255815460377 28.09469219305285 28.273778965154587 23.188970687188792 68-69 20.40611166101972 27.85983211672284 28.52834488141412 23.205711340843322 70-71 20.660467494718144 27.834521283674167 28.22497242569641 23.280038795911278 72-73 20.592275311279217 27.56681376277289 28.354543814337934 23.486367111609958 74-75 20.225300390552576 28.248574054470023 27.842471147155983 23.683654407821418 76-77 20.26683662502986 27.790037587329326 28.074049582160754 23.86907620548006 78-79 20.302524246259747 27.983068544346 27.959161672343093 23.75524553705116 80-81 20.47393813734188 27.764580986652575 27.73796588881468 24.02351498719086 82-83 20.307360135886793 27.69301466923448 27.91116919261395 24.08845600226478 84-85 20.124172771304597 27.71763129747936 27.67083101159391 24.487364919622127 86-87 20.098310696527093 27.680617964353893 27.655621182502454 24.565450156616564 88-89 20.0937898251644 27.574418665135514 27.327640189580286 25.0041513201198 90-91 20.10948751716215 27.372329609363426 27.419162767239207 25.099020106235216 92-93 20.32736341914898 27.36082743469129 27.35679034127893 24.955018804880797 94-95 19.98422043936557 27.072662235165296 27.499647377158908 25.443469948310227 96-97 19.797088909666886 27.092557999517542 27.56325271780845 25.547100373007126 98-99 19.810526126919985 27.19839234289508 27.106037785125388 25.885043745059555 100-101 19.81839912518977 26.82086825114709 27.574564713129828 25.786167910533308 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1617.0 1 1104.0 2 668.5 3 753.0 4 683.0 5 658.5 6 969.0 7 1661.0 8 2370.0 9 2626.0 10 2293.5 11 1942.5 12 1980.0 13 2076.5 14 2155.0 15 2289.5 16 2428.0 17 2610.5 18 2743.5 19 2885.0 20 3148.0 21 3521.5 22 3989.0 23 4537.5 24 5101.0 25 5985.0 26 7177.5 27 8481.5 28 9841.5 29 11338.5 30 12965.5 31 14875.5 32 16703.0 33 18074.0 34 19653.0 35 21130.0 36 23136.0 37 25457.5 38 26757.0 39 27025.5 40 27470.5 41 29006.0 42 30668.5 43 32233.5 44 33967.0 45 35482.5 46 36587.5 47 39502.5 48 44968.5 49 46222.0 50 42284.5 51 38020.5 52 34148.0 53 31855.0 54 30084.5 55 27194.0 56 24241.5 57 21254.0 58 18911.0 59 16683.0 60 13727.0 61 10899.5 62 8692.5 63 6690.5 64 4780.0 65 3485.5 66 2531.5 67 1914.0 68 1399.5 69 981.0 70 726.5 71 513.0 72 386.0 73 311.0 74 243.0 75 207.5 76 149.5 77 75.0 78 35.5 79 22.5 80 17.0 81 6.5 82 5.0 83 4.5 84 3.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2311605175045031 2 0.0 3 0.0 4 0.0 5 0.027511989146719644 6 0.004186607044066032 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.00338915808329155 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.987244803872412E-4 26-27 0.0014453762414037494 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008173851847938444 36-37 0.01913877505858758 38-39 0.014005197373601848 40-41 0.010217314809923056 42-43 0.009070981928809738 44-45 0.004037085363920817 46-47 4.984056004840515E-4 48-49 0.0018441007217909907 50-51 4.984056004840515E-5 52-53 0.0 54-55 0.0 56-57 0.0014453762414037494 58-59 5.980867205808619E-4 60-61 0.002591709122517068 62-63 9.96811200968103E-4 64-65 0.00164473848159737 66-67 0.005382780485227757 68-69 1.993622401936206E-4 70-71 4.984056004840515E-5 72-73 0.0 74-75 4.984056004840515E-4 76-77 0.05985851261813459 78-79 0.13905516253505038 80-81 8.971300808712927E-4 82-83 0.0010964923210649133 84-85 0.0 86-87 0.10665879850358703 88-89 0.35057849938048186 90-91 0.3894541362182379 92-93 1.993622401936206E-4 94-95 2.4920280024202577E-4 96-97 9.96811200968103E-5 98-99 0.0 100-101 9.96811200968103E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1003199.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.35725513812107 #Duplication Level Percentage of deduplicated Percentage of total 1 81.30803812394993 49.88838039951271 2 11.34342010315856 13.920022428167828 3 3.2226794891504476 5.932043029325811 4 1.3681228312775062 3.3577704647593007 5 0.6993280427368244 2.145442457172308 6 0.44226523630238246 1.628170856551601 7 0.2940303623617535 1.262862717324901 8 0.19828963291992405 0.973320607864972 9 0.1464903806032117 0.808942289215655 >10 0.8784737132910555 9.983269292036693 >50 0.06586148265546142 2.7345949959793963 >100 0.028611359555426916 3.088907366962441 >500 0.0019507742388599814 0.8414498934149589 >1k 0.00211333875876498 2.2555365623272245 >5k 3.2512903980999694E-4 1.1792866393841939 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6255 0.6235054062055484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5568 0.5550244766990398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3631 0.36194214707151817 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3444 0.3433017776134147 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2253 0.2245815635781136 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 1992 0.19856479123284612 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1983 0.19766766115197482 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1389 0.1384570758144695 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1277 0.12729279036362676 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1193 0.1189195762754947 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1127 0.1123406223491052 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1115 0.11114444890794348 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1110 0.11064604330745943 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1092 0.10885178314571685 RNA PCR Primer, Index 17 (95% over 21bp) TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1007 0.10037888793748798 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.9904336029043093E-4 0.0 12-13 0.0 0.0 0.0 2.9904336029043093E-4 0.0 14-15 9.96811200968103E-5 0.0 0.0 3.987244803872412E-4 0.0 16-17 9.96811200968103E-5 0.0 0.0 4.4856504043564637E-4 0.0 18-19 1.993622401936206E-4 0.0 0.0 4.984056004840515E-4 0.0 20-21 1.993622401936206E-4 0.0 0.0 4.984056004840515E-4 0.0 22-23 1.993622401936206E-4 0.0 0.0 0.0015450573615005598 0.0 24-25 1.993622401936206E-4 0.0 0.0 0.003837723123727197 0.0 26-27 2.9904336029043093E-4 0.0 0.0 0.008921460248664522 0.0 28-29 2.9904336029043093E-4 0.0 0.0 0.025568207304831843 0.0 30-31 2.9904336029043093E-4 0.0 0.0 0.0676834805457342 0.0 32-33 2.9904336029043093E-4 0.0 0.0 0.1597888355151869 0.0 34-35 2.9904336029043093E-4 0.0 0.0 0.3151418611860658 0.0 36-37 2.9904336029043093E-4 0.0 0.0 0.5451062052494071 0.0 38-39 4.4856504043564637E-4 0.0 0.0 0.8572077922725202 0.0 40-41 4.984056004840515E-4 0.0 0.0 1.1934322103590613 0.0 42-43 4.984056004840515E-4 0.0 0.0 1.5345410033303462 0.0 44-45 7.974489607744824E-4 0.0 0.0 1.9661104127894866 0.0 46-47 8.971300808712927E-4 0.0 0.0 2.4538501334231793 0.0 48-49 9.96811200968103E-4 0.0 0.0 2.9223015573181392 0.0 50-51 9.96811200968103E-4 0.0 0.0 3.4209065200423847 0.0 52-53 9.96811200968103E-4 0.0 0.0 3.896734346824508 0.0 54-55 9.96811200968103E-4 0.0 0.0 4.418415488851165 0.0 56-57 0.0010964923210649133 0.0 0.0 5.054181672828621 0.0 58-59 0.0010964923210649133 0.0 0.0 5.684764438561043 0.0 60-61 0.0010964923210649133 0.0 0.0 6.367629951784242 0.0 62-63 0.0010964923210649133 0.0 0.0 7.048152958685166 0.0 64-65 0.0010964923210649133 0.0 0.0 7.742132916799159 0.0 66-67 0.0010964923210649133 0.0 0.0 8.531607387965899 0.0 68-69 0.0010964923210649133 0.0 0.0 9.412987851861892 0.0 70-71 0.0010964923210649133 0.0 0.0 10.335985183398309 0.0 72-73 0.0010964923210649133 0.0 0.0 11.248914721804947 0.0 74-75 0.0010964923210649133 0.0 0.0 12.191200350080093 0.0 76-77 0.0010964923210649133 0.0 0.0 13.174504759275079 0.0 78-79 0.0010964923210649133 0.0 0.0 14.275233527944106 0.0 80-81 0.0010964923210649133 0.0 0.0 15.41668203417268 0.0 82-83 0.0010964923210649133 0.0 0.0 16.555488990718693 0.0 84-85 0.0010964923210649133 0.0 0.0 17.700775220070994 0.0 86-87 0.0010964923210649133 0.0 0.0 18.866097354562754 0.0 88-89 0.0010964923210649133 0.0 0.0 20.06595899716806 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 70 0.0083379345 27.178225 1 GAGTACT 5670 0.0 22.088923 12-13 AGTACTT 5820 0.0 20.704483 12-13 GGTATCA 9410 0.0 20.571404 1 GTACATG 4135 0.0 20.35902 1 GTACTTT 6295 0.0 19.858139 14-15 AGAGTAC 8495 0.0 19.573944 10-11 GTACTAC 195 1.02088725E-5 19.512571 1 TACATGG 4100 0.0 19.32834 2 GTATCAA 11920 0.0 19.312042 1 ACATGGG 4115 0.0 18.220034 3 TACTCCG 190 1.8796093E-4 17.482613 5 TACTTTT 6975 0.0 17.173977 14-15 TATCAAC 13465 0.0 17.056932 2 ACTTTTT 7370 0.0 17.025545 16-17 TCAACGC 13535 0.0 16.86354 4 ATCAACG 13565 0.0 16.861227 3 CAACGCA 13570 0.0 16.820045 5 AGTACAT 3450 0.0 16.780415 2 GAGTACA 4005 0.0 16.744635 1 >>END_MODULE