FastQCFastQC Report
Thu 26 May 2016
SRR938799_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938799_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122014
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71930.3389704309208139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57220.2696494933586677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35750.16847202704600442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38000.052.2665861
GTATCAA74000.044.9675371
ATCAACG93450.035.337193
TCAACGC93450.035.286494
TATCAAC93900.035.066932
CAACGCA96100.034.313455
AACGCAG98950.033.3054966
ACGCAGA113050.028.900897
CGCAGAG113050.028.4820378
GCAGAGT119100.025.8822449
GTACATG75550.023.3400731
TACATGG76200.023.0051612
GAGTACT68050.021.8493912-13
AGAGTAC108650.021.2678110-11
AGTACTT71250.021.2668412-13
ACATGGG79800.021.2548793
CAGAGTA113850.020.98267410-11
GTACTTT75250.019.5702714-15
GTGGTAT19650.019.2983931
CATGGGG50050.018.8376854
Produced by FastQC (version 0.11.5)