FastQCFastQC Report
Thu 26 May 2016
SRR938799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938799_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122014
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68510.3228536663754339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57940.27304249642085304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.17026277866215775No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT22650.10673822133124475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.054.8788761
GTATCAA74150.040.024181
ATCAACG93300.031.3803143
TCAACGC93150.031.3799084
TATCAAC93050.031.3626332
CAACGCA96900.030.1655145
AACGCAG99550.029.314856
ACGCAGA113450.025.5140347
CGCAGAG115900.024.892818
GTGGTAT17600.024.6305581
GTATACG1000.001626580723.8185631
GTACATG74700.023.404051
GCAGAGT124800.022.6993589
GAGTACT68850.022.19299712-13
TACATGG78000.022.0833932
ACATGGG78150.021.2516613
CAGAGTA118850.020.60224510-11
GTACTTT75150.020.07992714-15
TGGTATC20200.019.9673922
CATGGGG49100.019.9085454
Produced by FastQC (version 0.11.5)