##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938799_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2122014 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96730888674627 31.0 31.0 34.0 30.0 34.0 2 32.192417203656525 34.0 31.0 34.0 30.0 34.0 3 32.28294959411201 34.0 31.0 34.0 30.0 34.0 4 35.928877943312344 37.0 35.0 37.0 35.0 37.0 5 35.8275275280936 37.0 35.0 37.0 35.0 37.0 6 35.84750948862732 37.0 35.0 37.0 35.0 37.0 7 35.779746976221645 37.0 35.0 37.0 33.0 37.0 8 35.74795500877939 37.0 35.0 37.0 33.0 37.0 9 37.39379287789807 39.0 37.0 39.0 34.0 39.0 10-11 37.32354404824851 39.0 37.0 39.0 34.0 39.0 12-13 37.25100706215888 39.0 37.0 39.0 33.0 39.0 14-15 38.48053170242986 40.0 38.0 41.0 33.5 41.0 16-17 38.405851469405945 40.0 38.0 41.0 33.0 41.0 18-19 38.4158627134411 40.0 38.0 41.0 33.5 41.0 20-21 38.46602143058434 40.0 38.0 41.0 34.0 41.0 22-23 38.41518364157824 40.0 38.0 41.0 34.0 41.0 24-25 38.342200145710635 40.0 38.0 41.0 33.5 41.0 26-27 38.17532683573247 40.0 38.0 41.0 33.0 41.0 28-29 38.00578860459922 40.0 37.0 41.0 33.0 41.0 30-31 37.77445789707325 40.0 37.0 41.0 32.5 41.0 32-33 37.55035357919411 40.0 36.5 41.0 32.0 41.0 34-35 37.51498152227082 40.0 37.0 41.0 32.0 41.0 36-37 37.43944219972158 40.0 36.5 41.0 32.0 41.0 38-39 37.32990121648585 40.0 36.0 41.0 31.5 41.0 40-41 37.16525032351342 40.0 36.0 41.0 31.0 41.0 42-43 36.98466009178073 40.0 36.0 41.0 31.0 41.0 44-45 36.731780987307346 39.5 35.5 41.0 30.5 41.0 46-47 36.432680934244544 39.0 35.0 41.0 30.0 41.0 48-49 36.152919113634496 39.0 35.0 41.0 29.5 41.0 50-51 35.89665313235445 39.0 35.0 41.0 28.5 41.0 52-53 35.673275011380696 39.0 35.0 40.0 28.0 41.0 54-55 35.41145463696281 38.0 35.0 40.0 28.0 41.0 56-57 35.13392984212168 38.0 34.0 40.0 27.0 41.0 58-59 34.81227008869875 38.0 34.0 40.0 26.5 41.0 60-61 34.45258655220937 37.0 34.0 40.0 26.0 41.0 62-63 34.05403616564264 37.0 33.0 40.0 26.0 41.0 64-65 33.67837535473376 36.0 33.0 39.0 25.0 41.0 66-67 33.275250775913825 36.0 33.0 39.0 23.5 40.0 68-69 32.8197559017047 35.0 32.0 38.5 22.5 40.0 70-71 32.360262232011664 35.0 32.0 37.5 22.0 40.0 72-73 32.018489746061995 35.0 31.5 37.0 21.5 39.0 74-75 31.60677050198538 35.0 31.0 36.5 20.0 39.0 76-77 29.551004140406235 32.5 28.5 35.0 17.5 36.5 78-79 30.629120967156673 34.0 30.5 35.0 18.0 37.0 80-81 30.70244329208007 34.0 31.0 35.0 18.0 37.0 82-83 30.421066731887727 34.0 31.0 35.0 17.0 37.0 84-85 30.07360436830294 34.0 30.5 35.0 11.5 36.0 86-87 29.569315046931827 34.0 30.0 35.0 6.5 36.0 88-89 29.082019958398014 34.0 29.0 35.0 2.0 35.5 90-91 28.70214263430873 34.0 29.0 35.0 2.0 35.0 92-93 28.44246315057299 34.0 29.0 35.0 2.0 35.0 94-95 28.089495639519814 34.0 29.0 35.0 2.0 35.0 96-97 27.60378253866374 33.0 27.5 35.0 2.0 35.0 98-99 26.901829347025988 33.0 26.5 35.0 2.0 35.0 100-101 23.91202861998083 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16479756762013764 1101 2 0.14633244188136985 1101 3 0.12569587338681032 1101 4 0.10562149292790224 1101 5 0.01635002736475144 1101 6 0.19725659907593496 1101 7 0.15220108011322964 1101 8 0.15586013952579947 1101 9 0.36128336373632663 1101 10-11 0.33444856432177517 1101 12-13 0.3381526682049767 1101 14-15 0.35010765274275 1101 16-17 0.2973222302559577 1101 18-19 0.28507058108189653 1101 20-21 0.3677474058736081 1101 22-23 0.38951955968422425 1101 24-25 0.47582732334410593 1101 26-27 0.36818747181262523 1101 28-29 0.42410390726560365 1101 30-31 0.417574803608332 1101 32-33 0.4820353103221464 1101 34-35 0.41163402508907865 1101 36-37 0.40335034621935506 1101 38-39 0.5460874185651647 1101 40-41 0.5940299218801499 1101 42-43 0.3993366750953413 1101 44-45 0.5536938844511994 1101 46-47 0.6520684229417171 1101 48-49 0.5718015741412898 1101 50-51 0.5576847152185991 1101 52-53 0.6740691656007058 1101 54-55 0.7281308481880373 1101 56-57 0.7313298311703065 1101 58-59 0.7182524752872723 1101 60-61 0.6921637847007389 1101 62-63 0.7199082177647185 1101 64-65 0.6781483071638448 1101 66-67 0.598020538705768 1101 68-69 0.5200026505697863 1101 70-71 0.5210402105657153 1101 72-73 0.4816969954381065 1101 74-75 0.5821131476503716 1101 76-77 0.45429825829876336 1101 78-79 0.5194698771215798 1101 80-81 0.5995597364631209 1101 82-83 0.6719477706614647 1101 84-85 0.5943806550645228 1101 86-87 0.7525650538678512 1101 88-89 0.6709441570827543 1101 90-91 0.9647602944169833 1101 92-93 0.9535240399198059 1101 94-95 0.98544582716384 1101 96-97 0.8899028805474565 1101 98-99 0.8535186093579235 1101 100-101 1.5310652714272273 1102 1 0.06686300883514562 1102 2 0.10413987102761979 1102 3 0.17936769076801085 1102 4 0.12971886098904406 1102 5 0.08605071158449107 1102 6 0.15173738838917217 1102 7 0.07028976409267074 1102 8 -0.0026814869308680045 1102 9 0.015460325228033867 1102 10-11 0.14977506099654647 1102 12-13 0.07175331548477715 1102 14-15 0.15867746347987577 1102 16-17 0.2127231180927467 1102 18-19 0.19359936164202907 1102 20-21 0.19766607409285797 1102 22-23 0.16849370300352717 1102 24-25 -0.011898518381663337 1102 26-27 0.05325964529998117 1102 28-29 0.02813866915636254 1102 30-31 0.04862339944319416 1102 32-33 0.13347615976184812 1102 34-35 0.05700317525313636 1102 36-37 0.04872614301643807 1102 38-39 0.046862901692307446 1102 40-41 0.09250017372363573 1102 42-43 0.15341572656967628 1102 44-45 0.051051080901395096 1102 46-47 -0.04656773908786249 1102 48-49 0.022199796933868754 1102 50-51 -0.10585287852129 1102 52-53 0.0645113072992487 1102 54-55 0.05133897344127547 1102 56-57 0.06357929464642353 1102 58-59 0.019200131773935425 1102 60-61 -0.06788534925575362 1102 62-63 -0.10087963832545199 1102 64-65 -0.11042651411202087 1102 66-67 -0.027134922206279555 1102 68-69 -0.1229458469715965 1102 70-71 -0.02074755477234902 1102 72-73 0.06112477146191253 1102 74-75 0.18247193801428807 1102 76-77 0.031108922565827868 1102 78-79 -0.12875502448908094 1102 80-81 -0.09783235481049246 1102 82-83 0.010617881647455363 1102 84-85 0.025876109150988924 1102 86-87 -0.14262227253679427 1102 88-89 0.20934137265479436 1102 90-91 0.28143899745436585 1102 92-93 0.19448455508499407 1102 94-95 0.10762257391817442 1102 96-97 0.21675451108157873 1102 98-99 0.293031564540442 1102 100-101 0.4934559375093279 1103 1 0.13519218589249604 1103 2 0.13805508299769542 1103 3 0.09507841396003158 1103 4 0.10178868642545069 1103 5 0.1583699135795058 1103 6 0.09687454549640506 1103 7 0.17353165935451642 1103 8 0.20779232853048057 1103 9 0.17755508832030387 1103 10-11 0.07994962458757016 1103 12-13 0.168760796781541 1103 14-15 0.12201910936515503 1103 16-17 0.13466825524985637 1103 18-19 0.17863676812581986 1103 20-21 0.24754138581355534 1103 22-23 0.0829575434025358 1103 24-25 0.07862517239139777 1103 26-27 0.20313495702067286 1103 28-29 0.2578144796301842 1103 30-31 0.13440893560279932 1103 32-33 0.21452354130799023 1103 34-35 0.14129245206112273 1103 36-37 0.1307710307969927 1103 38-39 0.2012269914678626 1103 40-41 0.11594157023235852 1103 42-43 0.24294191110833196 1103 44-45 0.28371941007844725 1103 46-47 0.21228512874256467 1103 48-49 0.40299780192140844 1103 50-51 0.3567406376881834 1103 52-53 0.45678063398254665 1103 54-55 0.3186706442642162 1103 56-57 0.36308054152922153 1103 58-59 0.2999981367614666 1103 60-61 0.3465784911432479 1103 62-63 0.40934480057729417 1103 64-65 0.5411944335189034 1103 66-67 0.48084512766903487 1103 68-69 0.5506202378164318 1103 70-71 0.42339209610047135 1103 72-73 0.7267108063746335 1103 74-75 0.6243671998596731 1103 76-77 0.4730041844112094 1103 78-79 0.3298484667827424 1103 80-81 0.371993081598486 1103 82-83 0.30338596144795105 1103 84-85 0.3163997999240564 1103 86-87 0.4823153833235523 1103 88-89 0.42929149734307614 1103 90-91 0.6275736607960098 1103 92-93 0.6391229590750171 1103 94-95 0.5208395315241603 1103 96-97 0.5828392991115052 1103 98-99 0.8710864273833359 1103 100-101 1.2737551893796528 1104 1 -0.09604043621921932 1104 2 -0.08177146559613391 1104 3 -0.03139949434156364 1104 4 -0.03676260630407313 1104 5 -0.028934619933096428 1104 6 -0.06738774229254574 1104 7 -0.177613520312768 1104 8 -0.08887877863718074 1104 9 -0.07557324051074232 1104 10-11 -0.06353087139744673 1104 12-13 -0.040245058253816524 1104 14-15 -0.09716113625468381 1104 16-17 0.021970564392759684 1104 18-19 -0.0753778484546288 1104 20-21 0.01580951628025673 1104 22-23 -0.04210974378089816 1104 24-25 -0.0714660267663092 1104 26-27 0.06473034911526554 1104 28-29 0.08272272438004791 1104 30-31 -0.11820123866421284 1104 32-33 -0.06543278690023868 1104 34-35 -0.09533414299732357 1104 36-37 -0.16507495878801137 1104 38-39 0.02306016868340066 1104 40-41 0.024934202625608748 1104 42-43 0.029908834730889566 1104 44-45 -0.029538698366984306 1104 46-47 0.053200926463333076 1104 48-49 0.012490423192801359 1104 50-51 0.011041332927291592 1104 52-53 -0.0885758706923383 1104 54-55 -0.07396360262598733 1104 56-57 -0.18070049151750567 1104 58-59 -0.17042256569448 1104 60-61 -0.17777712131223922 1104 62-63 -0.3462237853908974 1104 64-65 -0.09417576338724842 1104 66-67 -0.23849947415643413 1104 68-69 -0.3542355612389514 1104 70-71 -0.34090652306025504 1104 72-73 -0.46846515362626207 1104 74-75 -0.5412148806180213 1104 76-77 -0.3974631062323475 1104 78-79 -0.36050197030590425 1104 80-81 -0.3984229249658746 1104 82-83 -0.40252320023654065 1104 84-85 -0.4072762675880348 1104 86-87 -0.3674886249906457 1104 88-89 -0.041517851929928185 1104 90-91 -0.009521781964778597 1104 92-93 -0.2033150489771991 1104 94-95 -0.03629266276712073 1104 96-97 -0.15491492780880733 1104 98-99 -0.0989464932882278 1104 100-101 0.38134103151366716 1105 1 -0.03228945031838393 1105 2 -0.060992024561556946 1105 3 -0.09740076727987201 1105 4 -0.029658097683793017 1105 5 -0.01660441530417245 1105 6 -0.1369247675966534 1105 7 -0.05857523917456575 1105 8 -0.06966473372394688 1105 9 -0.04570785301376645 1105 10-11 0.05409908322100421 1105 12-13 -0.08699771651227906 1105 14-15 -0.08275626382074108 1105 16-17 -0.07617071441397627 1105 18-19 -0.08334961254379891 1105 20-21 0.011984919662459959 1105 22-23 -0.04783640027498137 1105 24-25 -0.06772201263581934 1105 26-27 -0.021562172299923077 1105 28-29 -0.0024386309089834413 1105 30-31 -0.018517329192192733 1105 32-33 -0.12082282782024123 1105 34-35 0.10310583304715948 1105 36-37 -0.05424055302905373 1105 38-39 -0.09029590280350419 1105 40-41 0.06727289190386898 1105 42-43 0.20059723803166207 1105 44-45 0.18302935107428198 1105 46-47 0.19010346573915626 1105 48-49 0.09882883687655664 1105 50-51 0.09155600568530531 1105 52-53 0.13465243479165423 1105 54-55 0.18627218004557733 1105 56-57 0.14849595292779583 1105 58-59 0.1691104798706391 1105 60-61 0.22086215485708038 1105 62-63 0.04840672282563219 1105 64-65 -0.014813456333520492 1105 66-67 -0.08140554123478694 1105 68-69 -0.07844928574946408 1105 70-71 -0.2216825047091291 1105 72-73 -0.43977453593318927 1105 74-75 -0.3202504732642737 1105 76-77 -0.21484801299997258 1105 78-79 -0.311380861452335 1105 80-81 -0.01998455816210054 1105 82-83 -0.21100332057305238 1105 84-85 -0.008594471810315696 1105 86-87 0.10549576489271928 1105 88-89 0.23829827063832454 1105 90-91 0.16615683339441034 1105 92-93 0.21561318707817279 1105 94-95 0.1106931667603277 1105 96-97 0.006926746996409605 1105 98-99 0.2312683740722754 1105 100-101 0.535268518356677 1106 1 -0.06976138349143568 1106 2 -0.07502141835592369 1106 3 -0.06276554617853236 1106 4 0.007751648669646727 1106 5 -0.008421764812062804 1106 6 -0.1511629483176975 1106 7 -0.04313848435236167 1106 8 -0.11358588934894698 1106 9 -0.17781984982072174 1106 10-11 -0.05951931007965072 1106 12-13 0.010126982484599978 1106 14-15 0.05145294209849993 1106 16-17 -0.11077176823229706 1106 18-19 -0.22033346194103842 1106 20-21 -0.08942286697858748 1106 22-23 -0.1072241036471624 1106 24-25 -0.19670728925603953 1106 26-27 -0.14914824547891214 1106 28-29 -0.21083612149347175 1106 30-31 -0.09644013335903878 1106 32-33 -0.12570189218997996 1106 34-35 -0.24117671548693664 1106 36-37 -0.26317455271504286 1106 38-39 -0.1436575141940466 1106 40-41 -0.06659345892626334 1106 42-43 -0.0235179120963096 1106 44-45 0.11637063706933759 1106 46-47 0.19505121935262082 1106 48-49 0.03597676016780582 1106 50-51 0.05094966764248454 1106 52-53 -0.07116761310401643 1106 54-55 0.08394932499430041 1106 56-57 0.11533075257747072 1106 58-59 0.04192578359768362 1106 60-61 -0.22019159725443416 1106 62-63 -0.215883713740034 1106 64-65 -0.03198836823158757 1106 66-67 0.02351544603653366 1106 68-69 -0.026282228779727745 1106 70-71 -0.09198822740982138 1106 72-73 -0.12771599141688483 1106 74-75 0.031061432177711623 1106 76-77 -0.021580244594094466 1106 78-79 0.012223269076617527 1106 80-81 -0.04979959138830736 1106 82-83 0.14075654277673166 1106 84-85 0.02209070398685853 1106 86-87 0.2276712300879673 1106 88-89 0.2219337972353692 1106 90-91 0.2529257717637279 1106 92-93 0.12572745949711717 1106 94-95 0.264014061153393 1106 96-97 0.20932876610549656 1106 98-99 0.19073817350441047 1106 100-101 0.6631790033854976 1107 1 -0.08482652030549431 1107 2 -0.031162461098439564 1107 3 -0.09025081867199702 1107 4 -0.0901373779773138 1107 5 -0.03643539179149258 1107 6 -0.05573350289849088 1107 7 -0.06823589899001803 1107 8 0.06674996052919369 1107 9 -0.056917998758109434 1107 10-11 -0.014810511582197705 1107 12-13 -0.04375249088597144 1107 14-15 -0.04841516530851209 1107 16-17 0.019499207740608426 1107 18-19 0.05070316448674106 1107 20-21 0.05404716971795409 1107 22-23 -0.08659498589536696 1107 24-25 -0.09471400219465664 1107 26-27 -0.11782473478820776 1107 28-29 -0.1443577805680647 1107 30-31 -0.18855865726898458 1107 32-33 -0.05746079372924129 1107 34-35 -0.16553531020699097 1107 36-37 -0.16801401228520518 1107 38-39 -0.1246739981160232 1107 40-41 -0.13191188763553896 1107 42-43 -0.0855104288809585 1107 44-45 -0.17609040630046735 1107 46-47 -0.24800665010808132 1107 48-49 -0.4172255846098949 1107 50-51 -0.28928571416570037 1107 52-53 -0.2552797792328718 1107 54-55 -0.2907116609968057 1107 56-57 -0.41136279536326015 1107 58-59 -0.31734269797010484 1107 60-61 -0.4014997485349383 1107 62-63 -0.3926463098401243 1107 64-65 -0.3584814597257022 1107 66-67 -0.46680743367299016 1107 68-69 -0.18432145016371493 1107 70-71 -0.2033679360145939 1107 72-73 -0.2637684246611016 1107 74-75 -0.24815869261634305 1107 76-77 -0.24431394747576007 1107 78-79 -0.15522382167581839 1107 80-81 -0.14583338116825573 1107 82-83 -0.07105572002877025 1107 84-85 -0.10205749448808987 1107 86-87 -0.10978319904934253 1107 88-89 0.09963472415040187 1107 90-91 0.14404173669445797 1107 92-93 0.0028881549047881094 1107 94-95 0.1067370447349667 1107 96-97 0.0990535051800201 1107 98-99 0.036941585599695514 1107 100-101 0.3806142863030644 1108 1 -0.17987414086257303 1108 2 -0.1343856813866111 1108 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7 401.0 8 949.0 9 1657.0 10 2718.0 11 4125.0 12 5617.0 13 7681.0 14 8722.0 15 11161.0 16 13744.0 17 18188.0 18 17491.0 19 12714.0 20 12019.0 21 13031.0 22 13711.0 23 15166.0 24 17589.0 25 21102.0 26 25103.0 27 30113.0 28 37776.0 29 45519.0 30 55593.0 31 67555.0 32 82802.0 33 104770.0 34 139180.0 35 192347.0 36 290392.0 37 449595.0 38 368344.0 39 34875.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.20639747779732 19.541866710065914 14.598184801306518 23.653551010830256 2 16.398666549796562 21.30834198077864 37.97628102359362 24.316710445831177 3 18.547615614223094 24.868356193691465 30.84673333917684 25.737294852908605 4 13.846609871565411 17.652286931188954 36.048819659059745 32.452283538185895 5 14.023941406607118 36.919313444680384 34.113347037295696 14.943398111416794 6 31.56817061527398 35.93939531030427 17.565953853273353 14.9264802211484 7 27.70075974993567 32.90680457339113 21.35141426965138 18.04102140702182 8 26.18371038079862 34.312874467369205 20.466076095633674 19.037339056198498 9 26.46773301212904 16.31516097443278 20.561928432140412 36.655177581297764 10-11 24.81001539103889 26.236726053645263 27.67314447501289 21.28011408030296 12-13 25.55466175058223 23.91324939420758 27.857662579040475 22.674426276169715 14-15 23.035342418111878 25.43801610830481 25.86277827966019 25.663863193923124 16-17 22.398410535106024 28.260104280358085 26.712416563460785 22.629068621075113 18-19 22.34855160908761 26.880551929538505 28.812022563512897 21.95887389786099 20-21 23.09562990630599 26.240684557217815 28.70867015957482 21.955015376901375 22-23 22.965494101358427 26.07949334924275 28.557964273562757 22.397048275836067 24-25 22.74551440282675 26.312620934640396 28.648420792699763 22.293443869833094 26-27 22.54102358564588 26.402478592241042 28.654634683797585 22.401863138315495 28-29 22.458829206593357 26.5658709131985 28.595193057161733 22.380106823046408 30-31 22.84308680338584 26.257578884964943 28.52879387223647 22.370540439412746 32-33 22.628620734830214 26.416248903164636 28.34276776684791 22.612362595157244 34-35 22.898493077118662 26.503429451930998 28.315535498544776 22.28254197240556 36-37 23.06616218330845 26.232152683593018 28.209229405990875 22.492455727107654 38-39 22.733084767394786 26.26918739109923 28.311019426695903 22.68670841481008 40-41 22.888788765530837 26.14650016236008 28.362868414814212 22.601842657294867 42-43 22.814443316282084 26.262567235164667 28.066509059059968 22.856480389493278 44-45 22.645000743409284 26.330449313742516 28.076153385952416 22.948396556895787 46-47 22.886654974786584 26.348766285160764 27.706056426870024 23.058522313182632 48-49 22.824708036799002 26.469476638702666 27.596095030475766 23.109720294022566 50-51 22.753473309539782 26.627547975297873 27.48925720433543 23.129721510826915 52-53 22.852134811551668 26.655809056867675 27.378542271634398 23.11351385994626 54-55 22.53540740070518 26.58759555780499 27.42029505931629 23.456701982173538 56-57 22.539436591841525 26.74725520189782 27.236083268065148 23.477224938195505 58-59 22.605232359090323 26.52802901404873 27.40736185303862 23.459376773822324 60-61 22.64673234208364 26.636432632116424 27.451629982353925 23.26520504344601 62-63 22.37438113037897 26.582506053211713 27.49852262991667 23.54459018649264 64-65 22.237624256955893 26.51320867817083 27.4595973447866 23.78956972008667 66-67 22.19351491276002 26.652463028244927 27.243181699832448 23.91084035916261 68-69 22.19327153311571 26.49299080725297 27.326780078595704 23.986957581035618 70-71 22.187440724512868 26.395920786153482 27.15292692783029 24.263711561503364 72-73 22.147921738499367 26.505786483972305 26.985448729367477 24.360843048160852 74-75 22.046202334197606 26.586252494092875 26.862499493405796 24.505045678303723 76-77 22.011511454493952 26.330276332929774 27.083806530390632 24.574405682185642 78-79 21.83645323634005 26.395824341961198 27.11596695622126 24.651755465477493 80-81 21.970351722798927 26.410312477026523 26.951025724642463 24.66831007553209 82-83 21.93548204677255 26.314859374160587 26.9629700840805 24.786688494986368 84-85 21.772759274915245 26.2008403337584 26.885590764245666 25.14080962708069 86-87 21.659294149851863 26.107865682930253 27.08606435033162 25.14677581688627 88-89 21.626578484012324 25.991369283462344 26.95881426228981 25.42323797023552 90-91 21.650900574138756 25.889527555966595 27.016019080933763 25.443552788960883 92-93 21.476743320260848 25.859725713402455 26.95705589124294 25.706475075093753 94-95 21.39531628804435 25.66244276956768 27.088965268128597 25.85327567425938 96-97 21.359354839317742 25.41639687579818 27.12185216497158 26.1023961199125 98-99 21.172943137705364 25.533025842213462 27.03466638391621 26.25936463616496 100-101 21.22593750566977 25.204683033769314 27.355200905937583 26.214178554623334 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 562.0 1 431.5 2 357.0 3 452.0 4 447.0 5 448.5 6 656.0 7 1121.0 8 1741.5 9 2121.5 10 1957.0 11 1758.5 12 1870.0 13 2098.0 14 2253.5 15 2385.5 16 2644.0 17 2853.5 18 3084.5 19 3396.5 20 3761.0 21 4271.0 22 4889.0 23 5633.5 24 6606.5 25 8130.5 26 10089.0 27 12545.5 28 15038.0 29 17707.0 30 21030.0 31 24558.0 32 28563.5 33 31714.5 34 34438.0 35 37705.0 36 41890.5 37 46138.5 38 49940.5 39 52585.0 40 54538.0 41 57294.0 42 60030.5 43 63925.0 44 68229.5 45 72282.0 46 77322.5 47 81079.5 48 83303.5 49 85393.5 50 86176.0 51 86201.0 52 85694.0 53 85124.0 54 83236.0 55 77456.5 56 69839.0 57 62367.0 58 56221.0 59 49783.0 60 41803.0 61 34296.5 62 27945.0 63 21857.0 64 16057.0 65 11730.5 66 8569.0 67 6591.0 68 5014.0 69 3578.5 70 2621.0 71 1968.5 72 1440.0 73 1053.5 74 793.5 75 586.5 76 399.0 77 232.5 78 134.5 79 80.0 80 53.5 81 35.5 82 24.0 83 18.5 84 14.0 85 8.5 86 3.5 87 3.0 88 6.0 89 5.5 90 2.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39142060325709443 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.4137512759105264E-4 16-17 0.001979251786274737 18-19 1.8850017012140354E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.4137512759105264E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008176194879015879 36-37 0.017506953300025354 38-39 0.011451385334875264 40-41 0.008812382953175615 42-43 0.0036521907961021938 44-45 0.0015786889247667547 46-47 0.0013430637121150002 48-49 0.0 50-51 4.948129465686843E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.534378189776316E-4 60-61 5.890630316293861E-4 62-63 0.0 64-65 0.0 66-67 0.0010603134569328947 68-69 7.068756379552632E-4 70-71 0.0017200640523578074 72-73 0.0 74-75 0.0 76-77 0.10016427789826081 78-79 0.0361684701420443 80-81 9.425008506070177E-5 82-83 0.0 84-85 0.0 86-87 0.15181332451152538 88-89 0.3303465481377597 90-91 0.28157212911884655 92-93 0.0 94-95 2.3562521265175442E-5 96-97 0.0 98-99 7.068756379552632E-5 100-101 6.361880741597369E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2122014.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.06751628980082 #Duplication Level Percentage of deduplicated Percentage of total 1 76.02238671767915 43.384087924006934 2 13.255890384988051 15.129614809622394 3 4.369603396712513 7.480872390655811 4 2.0508532955637055 4.6814841541029395 5 1.0994308601150056 3.13708942595614 6 0.7129815706639588 2.441285243891595 7 0.48663636956415385 1.9439790263118322 8 0.33675903693261244 1.5374401460711598 9 0.25151927328172846 1.2918222202683514 >10 1.3318264078630697 13.159529200223869 >50 0.05845352076723518 2.2471028418618935 >100 0.02233669884960843 2.2665724852925213 >500 8.265418870824331E-4 0.31376614321031493 >1k 3.306167548329539E-4 0.3889058705305064 >5k 1.6530837741647695E-4 0.596448117993724 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6851 0.3228536663754339 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5794 0.27304249642085304 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3613 0.17026277866215775 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2265 0.10673822133124475 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.068756379552632E-5 0.0 12-13 0.0 0.0 0.0 9.425008506070177E-5 0.0 14-15 0.0 0.0 0.0 1.8850017012140354E-4 0.0 16-17 0.0 0.0 0.0 1.8850017012140354E-4 4.7125042530350884E-5 18-19 0.0 0.0 0.0 1.8850017012140354E-4 4.7125042530350884E-5 20-21 0.0 0.0 0.0 3.5343781897763165E-4 4.7125042530350884E-5 22-23 0.0 0.0 0.0 8.01125723015965E-4 4.7125042530350884E-5 24-25 0.0 0.0 0.0 0.0019556892650095616 4.7125042530350884E-5 26-27 0.0 0.0 0.0 0.00450044156164851 4.7125042530350884E-5 28-29 0.0 0.0 0.0 0.013807637461392809 4.7125042530350884E-5 30-31 0.0 0.0 0.0 0.0385718473110922 4.7125042530350884E-5 32-33 0.0 0.0 0.0 0.0908570819985165 4.7125042530350884E-5 34-35 0.0 0.0 0.0 0.17982916229581897 9.425008506070177E-5 36-37 0.0 0.0 0.0 0.31347578291189404 9.425008506070177E-5 38-39 0.0 0.0 0.0 0.5089504593277896 9.425008506070177E-5 40-41 0.0 0.0 0.0 0.7251365919357742 9.425008506070177E-5 42-43 0.0 0.0 0.0 0.9372699708861487 1.178126063258772E-4 44-45 0.0 0.0 0.0 1.197305955568625 1.4137512759105264E-4 46-47 0.0 0.0 0.0 1.5020636056124042 1.4137512759105264E-4 48-49 0.0 0.0 0.0 1.8207938307664322 1.4137512759105264E-4 50-51 0.0 0.0 0.0 2.161507888260869 1.4137512759105264E-4 52-53 0.0 0.0 0.0 2.4980042544488397 1.4137512759105264E-4 54-55 0.0 0.0 0.0 2.8627756461550207 1.649376488562281E-4 56-57 0.0 0.0 0.0 3.286948153970709 1.8850017012140354E-4 58-59 0.0 0.0 0.0 3.750163759522793 1.8850017012140354E-4 60-61 0.0 0.0 0.0 4.250938023971567 1.8850017012140354E-4 62-63 0.0 0.0 0.0 4.742522905126922 1.8850017012140354E-4 64-65 0.0 0.0 0.0 5.263820125597664 1.8850017012140354E-4 66-67 0.0 0.0 0.0 5.839641020276021 1.8850017012140354E-4 68-69 0.0 0.0 0.0 6.489424669205764 1.8850017012140354E-4 70-71 0.0 0.0 0.0 7.183505858114037 2.356252126517544E-4 72-73 0.0 0.0 0.0 7.884797178529453 3.063127764472807E-4 74-75 0.0 0.0 0.0 8.605032766042072 3.298752977124562E-4 76-77 0.0 0.0 0.0 9.373972085009807 3.298752977124562E-4 78-79 0.0 0.0 0.0 10.214588593666207 3.298752977124562E-4 80-81 0.0 0.0 0.0 11.098088891025224 3.5343781897763165E-4 82-83 0.0 0.0 0.0 12.003196020384408 3.770003402428071E-4 84-85 0.0 0.0 0.0 12.930051356871349 3.770003402428071E-4 86-87 0.0 0.0 0.0 13.88763222108808 3.770003402428071E-4 88-89 0.0 0.0 0.0 14.880886742500286 3.770003402428071E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4210 0.0 54.878876 1 GTATCAA 7415 0.0 40.02418 1 ATCAACG 9330 0.0 31.380314 3 TCAACGC 9315 0.0 31.379908 4 TATCAAC 9305 0.0 31.362633 2 CAACGCA 9690 0.0 30.165514 5 AACGCAG 9955 0.0 29.31485 6 ACGCAGA 11345 0.0 25.514034 7 CGCAGAG 11590 0.0 24.89281 8 GTGGTAT 1760 0.0 24.630558 1 GTATACG 100 0.0016265807 23.818563 1 GTACATG 7470 0.0 23.40405 1 GCAGAGT 12480 0.0 22.699358 9 GAGTACT 6885 0.0 22.192997 12-13 TACATGG 7800 0.0 22.083393 2 ACATGGG 7815 0.0 21.251661 3 CAGAGTA 11885 0.0 20.602245 10-11 GTACTTT 7515 0.0 20.079927 14-15 TGGTATC 2020 0.0 19.967392 2 CATGGGG 4910 0.0 19.908545 4 >>END_MODULE