FastQCFastQC Report
Thu 26 May 2016
SRR938798_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938798_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121728
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73560.34669854005791506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55470.2614378468870656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36710.17301935026544402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43550.053.4623571
GTATCAA74550.039.9631841
TCAACGC95200.031.2964694
ATCAACG95050.031.1961153
TATCAAC95450.031.0156782
CAACGCA96950.030.6336775
AACGCAG100200.029.543286
ACGCAGA113450.025.967437
CGCAGAG114850.025.4030558
GTACATG79000.024.1790451
GCAGAGT121950.023.378919
TACATGG80100.023.3365332
ACATGGG81950.022.230793
GAGTACT68700.021.83145112-13
CAGAGTA117900.020.97226710-11
CATGGGG49950.020.2309724
GTGGTAT19850.019.8681661
GTACTTT74800.019.60737414-15
AGAGTAC112350.019.24140210-11
AGTACTT72100.019.15622712-13
Produced by FastQC (version 0.11.5)