##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938798_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2121728 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6174434234737 33.0 31.0 34.0 30.0 34.0 2 31.680845047055985 33.0 31.0 34.0 30.0 34.0 3 31.69936579995174 33.0 31.0 34.0 28.0 34.0 4 35.26160987647804 37.0 35.0 37.0 33.0 37.0 5 35.288259381032816 37.0 35.0 37.0 33.0 37.0 6 35.343009094473935 37.0 35.0 37.0 33.0 37.0 7 35.25470277057191 37.0 35.0 37.0 33.0 37.0 8 35.227928367820944 37.0 35.0 37.0 32.0 37.0 9 36.811205300585186 39.0 37.0 39.0 33.0 39.0 10-11 36.7033592430321 39.0 37.0 39.0 32.0 39.0 12-13 36.59914395247647 39.0 37.0 39.0 32.0 39.0 14-15 37.77969136477438 40.0 38.0 41.0 32.0 41.0 16-17 37.66053047327462 40.0 37.0 41.0 32.0 41.0 18-19 37.594554768566 40.0 37.0 41.0 32.0 41.0 20-21 37.480472284854606 40.0 37.0 41.0 32.0 41.0 22-23 37.47221368620295 40.0 37.0 41.0 32.0 41.0 24-25 37.40244343289997 40.0 37.0 41.0 32.0 41.0 26-27 37.27228325214165 40.0 37.0 41.0 31.0 41.0 28-29 37.09724220069679 40.0 37.0 41.0 31.0 41.0 30-31 36.870577661227074 39.5 36.0 41.0 30.0 41.0 32-33 36.67941814407879 39.0 36.0 41.0 30.0 41.0 34-35 36.39392207672237 39.0 35.0 41.0 30.0 41.0 36-37 36.18708783595258 39.0 35.0 41.0 29.5 41.0 38-39 36.01808125263936 39.0 35.0 41.0 29.0 41.0 40-41 35.905721421407456 39.0 35.0 41.0 28.5 41.0 42-43 35.9143238435841 39.0 35.0 41.0 28.5 41.0 44-45 35.73915082423383 39.0 35.0 41.0 27.5 41.0 46-47 35.43232732942205 39.0 35.0 41.0 26.5 41.0 48-49 35.140720205417466 39.0 35.0 41.0 25.0 41.0 50-51 33.98148372458675 38.0 33.5 40.0 23.5 40.5 52-53 34.02953771642737 38.0 33.5 39.5 23.5 40.5 54-55 34.51992809634412 38.0 34.0 40.0 24.0 41.0 56-57 34.40452169175313 38.0 34.0 40.0 23.0 41.0 58-59 34.18895871666868 38.0 34.0 40.0 23.0 41.0 60-61 33.87891473365106 37.5 33.5 40.0 22.0 41.0 62-63 33.46694863809122 37.0 33.0 40.0 21.5 41.0 64-65 33.06870107761221 36.0 33.0 39.5 20.0 41.0 66-67 32.68050829324022 36.0 32.0 39.0 18.5 41.0 68-69 32.247548931814066 35.0 32.0 39.0 17.5 40.0 70-71 31.832068012487934 35.0 31.0 38.0 14.0 40.0 72-73 31.400626046316965 35.0 31.0 37.0 10.0 39.5 74-75 30.93487973010678 35.0 31.0 37.0 7.5 39.0 76-77 30.4752197265625 35.0 30.0 36.0 4.5 39.0 78-79 30.039820608485158 34.0 30.0 36.0 2.0 38.0 80-81 29.587084913806105 34.0 29.5 35.0 2.0 37.0 82-83 29.17438286151665 34.0 29.0 35.0 2.0 37.0 84-85 28.825092801716337 34.0 29.0 35.0 2.0 36.0 86-87 28.394619385708253 34.0 29.0 35.0 2.0 36.0 88-89 27.934917435222612 34.0 27.5 35.0 2.0 35.5 90-91 27.479090156702462 34.0 26.5 35.0 2.0 35.0 92-93 26.999864497239987 33.5 25.5 35.0 2.0 35.0 94-95 26.46511239895029 33.0 25.0 35.0 2.0 35.0 96-97 25.851946385210546 33.0 23.5 35.0 2.0 35.0 98-99 25.068654653188347 32.5 17.0 35.0 2.0 35.0 100-101 22.669984324098095 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30446455093668945 1101 2 0.3434244676336675 1101 3 0.31955762234951024 1101 4 0.11882165211352458 1101 5 0.05745984169820417 1101 6 0.1710290392317262 1101 7 0.22737130207644896 1101 8 0.2567984375445391 1101 9 0.31608917291661953 1101 10-11 0.29468092128376355 1101 12-13 0.18713257327814858 1101 14-15 0.26364255663573033 1101 16-17 0.40460363352062245 1101 18-19 0.4385995466627506 1101 20-21 0.34726274996231155 1101 22-23 0.3404263384715591 1101 24-25 0.41698357975256783 1101 26-27 0.4156258170623133 1101 28-29 0.455325440709764 1101 30-31 0.39642537326483307 1101 32-33 0.46958310408124504 1101 34-35 0.388249350631348 1101 36-37 0.3204480861572563 1101 38-39 0.2878956459921227 1101 40-41 0.4650793637481314 1101 42-43 0.576133762652411 1101 44-45 0.5484362438169725 1101 46-47 0.5799746319474224 1101 48-49 0.32923603553934555 1101 50-51 0.395208354967167 1101 52-53 0.41476117535784596 1101 54-55 0.46298578050069494 1101 56-57 0.3488503885970644 1101 58-59 0.3915026980491305 1101 60-61 0.20213482352769319 1101 62-63 0.21908317694194324 1101 64-65 0.19898037646704125 1101 66-67 0.0990030778848876 1101 68-69 0.282048113532106 1101 70-71 0.24347760402514496 1101 72-73 0.3520030225879829 1101 74-75 0.29209720618098345 1101 76-77 0.12456347270479284 1101 78-79 -0.07518553153156304 1101 80-81 -0.18811008433189613 1101 82-83 -0.057393830182913774 1101 84-85 -0.0029819501935826054 1101 86-87 -0.21840794068203806 1101 88-89 -0.14300475153940795 1101 90-91 -0.13570497654591307 1101 92-93 -0.19044060458966783 1101 94-95 -0.13585993777411787 1101 96-97 -0.06675224626993881 1101 98-99 0.1285604726145273 1101 100-101 0.3908487816311421 1102 1 0.06893715663410305 1102 2 0.11326061294041168 1102 3 0.18061118781773544 1102 4 0.37030846037731635 1102 5 0.2977562685763573 1102 6 0.2923178458946296 1102 7 0.2087024835858884 1102 8 0.3784308760014099 1102 9 0.4284565292418847 1102 10-11 0.31139175945457964 1102 12-13 0.40000225680768864 1102 14-15 0.4155615719936705 1102 16-17 0.6720258255502358 1102 18-19 0.5018710511043736 1102 20-21 0.43197075064765045 1102 22-23 0.4422512876296665 1102 24-25 0.3365486284101067 1102 26-27 0.2741116360029636 1102 28-29 0.466746511971067 1102 30-31 0.5433598051946191 1102 32-33 0.41817807057233836 1102 34-35 0.3783587625139475 1102 36-37 0.4767419187355202 1102 38-39 0.46643447073774524 1102 40-41 0.5518978163429296 1102 42-43 0.6852202764021271 1102 44-45 0.7163091461911719 1102 46-47 0.7064026563457588 1102 48-49 0.6442907607047985 1102 50-51 0.6856780275389411 1102 52-53 0.5848531461246367 1102 54-55 0.4607000283159337 1102 56-57 0.5138790770458215 1102 58-59 0.5064057205911396 1102 60-61 0.4682018326887274 1102 62-63 0.11125644618514485 1102 64-65 0.12611445606609806 1102 66-67 0.09922595794705558 1102 68-69 0.10829946793399614 1102 70-71 0.08770426547886245 1102 72-73 -0.04603409493240562 1102 74-75 -0.0838020938434525 1102 76-77 0.08207640515519188 1102 78-79 0.01753377584131144 1102 80-81 -0.07556322244712277 1102 82-83 -0.14722566927864733 1102 84-85 -0.07883914931571212 1102 86-87 -0.14199492314071804 1102 88-89 -0.25124408075091864 1102 90-91 -0.40857241084916396 1102 92-93 -0.44463910025137565 1102 94-95 -0.8199922710988794 1102 96-97 -0.9216507174929376 1102 98-99 -1.0582126590317742 1102 100-101 -0.7660078381229809 1103 1 0.2872398585707252 1103 2 0.2731172115311189 1103 3 0.44279078471079814 1103 4 0.35648201440957195 1103 5 0.35343708272872476 1103 6 0.22148538461067346 1103 7 0.2135828211460975 1103 8 0.30828891303217176 1103 9 0.39827283032268923 1103 10-11 0.3862135965285205 1103 12-13 0.3673181205885925 1103 14-15 0.3430448880900485 1103 16-17 0.481057251581106 1103 18-19 0.6073293072677117 1103 20-21 0.519810470286032 1103 22-23 0.5186424604574142 1103 24-25 0.4756781532281451 1103 26-27 0.5264336808385011 1103 28-29 0.5789971009428214 1103 30-31 0.7392622756624547 1103 32-33 0.4982768167752454 1103 34-35 0.542953855761823 1103 36-37 0.6421864214343032 1103 38-39 0.6033711395883614 1103 40-41 0.591283176319024 1103 42-43 0.6342832539448082 1103 44-45 0.5550542473139828 1103 46-47 0.5023336837333261 1103 48-49 0.44614328787448443 1103 50-51 0.5515461990979844 1103 52-53 0.595196730834985 1103 54-55 0.6941994610620341 1103 56-57 0.6710194723272664 1103 58-59 0.565009557953708 1103 60-61 0.51374752999903 1103 62-63 0.5327711777183097 1103 64-65 0.33693373831982143 1103 66-67 0.3923855751195333 1103 68-69 0.32131549064957454 1103 70-71 0.38846195377620063 1103 72-73 0.3299717831316613 1103 74-75 0.2235339269322374 1103 76-77 0.2639007615003486 1103 78-79 0.3177916591873533 1103 80-81 0.515768893116821 1103 82-83 0.5883131230485112 1103 84-85 0.5575292062271195 1103 86-87 0.4947531711616229 1103 88-89 0.6402416253689509 1103 90-91 0.4375881901598184 1103 92-93 0.07948690615639364 1103 94-95 0.1313818629237069 1103 96-97 0.09439071428933588 1103 98-99 0.3259486562533347 1103 100-101 0.62053761505274 1104 1 -0.06800329256158477 1104 2 0.012993542444885264 1104 3 0.11566542387007672 1104 4 0.12504299286597842 1104 5 0.0018521679533805013 1104 6 0.16183984325736134 1104 7 0.09549204648681808 1104 8 0.1388811988080505 1104 9 0.1656894207470927 1104 10-11 0.07096903463399684 1104 12-13 0.21245111857199106 1104 14-15 0.2880678559021632 1104 16-17 0.2171704671049639 1104 18-19 0.2664772068910466 1104 20-21 0.3116578425209795 1104 22-23 0.23127553443170967 1104 24-25 0.1881079660677969 1104 26-27 0.14462979859723646 1104 28-29 0.17568176333558938 1104 30-31 0.2846183968274403 1104 32-33 0.21449735696258898 1104 34-35 0.21331487862939724 1104 36-37 0.38577157195543066 1104 38-39 0.3707689353188073 1104 40-41 0.24127759591915776 1104 42-43 0.2795655421268677 1104 44-45 0.391654454241241 1104 46-47 0.45338533967348127 1104 48-49 0.3964506971373041 1104 50-51 0.5353671000506495 1104 52-53 0.5969158652488389 1104 54-55 0.5179584833852573 1104 56-57 0.2883241183060079 1104 58-59 0.395014103485245 1104 60-61 0.23956589390004268 1104 62-63 0.3246331680485568 1104 64-65 0.6183598008059121 1104 66-67 0.3736923957139666 1104 68-69 0.27308296919287756 1104 70-71 0.5324663187858647 1104 72-73 0.35491060176217104 1104 74-75 0.28490730394660346 1104 76-77 0.3399027913272974 1104 78-79 0.0965666256759512 1104 80-81 0.1539130261442736 1104 82-83 -0.06309542324688167 1104 84-85 0.16349113838662888 1104 86-87 0.08766044811907747 1104 88-89 0.17258926595035717 1104 90-91 0.08426209781104532 1104 92-93 0.13935937603673665 1104 94-95 0.13613448414542617 1104 96-97 -0.0024028877184001374 1104 98-99 0.2966809091315348 1104 100-101 0.6539956507937461 1105 1 0.045864143866932494 1105 2 0.21359326286672697 1105 3 0.17163705358403192 1105 4 0.19433681102908906 1105 5 0.1444668497448589 1105 6 0.11359178217356458 1105 7 0.14058534960877722 1105 8 0.14114636920117363 1105 9 0.13230894473579724 1105 10-11 0.2720709122146374 1105 12-13 0.21428287680267033 1105 14-15 0.2988571782108025 1105 16-17 0.2749661155466967 1105 18-19 0.34536368363487213 1105 20-21 0.30968119624334633 1105 22-23 0.17171391353395649 1105 24-25 0.22263514048682964 1105 26-27 0.2311718956883979 1105 28-29 0.34577099752770835 1105 30-31 0.3491339824790387 1105 32-33 0.263144912975541 1105 34-35 0.1662513029380861 1105 36-37 0.42781653528042796 1105 38-39 0.3609943984199333 1105 40-41 0.2773407202153422 1105 42-43 0.1267278875331428 1105 44-45 0.20849627268753324 1105 46-47 0.22571727693707544 1105 48-49 0.06551649588700315 1105 50-51 0.14688445918657322 1105 52-53 0.08219931429912464 1105 54-55 0.11296941770942937 1105 56-57 -0.06474483360823768 1105 58-59 0.03622065101365024 1105 60-61 -0.03129121279606295 1105 62-63 -0.13250887937319789 1105 64-65 -0.09453616866584724 1105 66-67 -0.14630966663592204 1105 68-69 -0.03842621793851464 1105 70-71 -0.11443570616401644 1105 72-73 -0.02666635382241722 1105 74-75 -0.09656937954892797 1105 76-77 -0.11496907778769128 1105 78-79 -0.10334462043637416 1105 80-81 0.01696001835719585 1105 82-83 -0.2303673645668809 1105 84-85 -0.1976103931927078 1105 86-87 -0.15348789379486405 1105 88-89 -0.02817924392444837 1105 90-91 0.11109188133032077 1105 92-93 -0.15530364486880188 1105 94-95 -0.04077188630769868 1105 96-97 0.07688604317626258 1105 98-99 0.0660899320716446 1105 100-101 0.6557175763729646 1106 1 0.13072392278728273 1106 2 0.1348101280706544 1106 3 0.1604095661730689 1106 4 0.17152886635992814 1106 5 0.2185355523786896 1106 6 0.18260897637452445 1106 7 0.1691672982142478 1106 8 0.23219735844386946 1106 9 0.16848338733482393 1106 10-11 0.19494931840677765 1106 12-13 0.2865469962587923 1106 14-15 0.37972833163414776 1106 16-17 0.36695052129911687 1106 18-19 0.38585753679411283 1106 20-21 0.30645678688838984 1106 22-23 0.3024865930055469 1106 24-25 0.3376864237606938 1106 26-27 0.2608044759310886 1106 28-29 0.0864338523849284 1106 30-31 0.11104224475484159 1106 32-33 0.2913103174273246 1106 34-35 0.38142814546717574 1106 36-37 0.5209820324447492 1106 38-39 0.5865341840155835 1106 40-41 0.6857109398910595 1106 42-43 0.6011034686420373 1106 44-45 0.6891630612049511 1106 46-47 0.7191535609129005 1106 48-49 0.6966832036120749 1106 50-51 0.5439682527713927 1106 52-53 0.24456809295722337 1106 54-55 0.2536173061153306 1106 56-57 0.5337589186003058 1106 58-59 0.5626172328351018 1106 60-61 0.6473433170586986 1106 62-63 0.6179146182202473 1106 64-65 0.5692471383106366 1106 66-67 0.3837199219058647 1106 68-69 0.5697858606499224 1106 70-71 0.5360552729067933 1106 72-73 0.5075574833637866 1106 74-75 0.45444370351355445 1106 76-77 0.4469503099917347 1106 78-79 0.42650980764651436 1106 80-81 0.3954936902237094 1106 82-83 0.14095890142271728 1106 84-85 0.44542418063428 1106 86-87 0.47292365447790274 1106 88-89 0.6331097648726463 1106 90-91 0.6770041991170892 1106 92-93 0.6103273566119505 1106 94-95 0.6520847456844798 1106 96-97 0.6614941738002678 1106 98-99 0.5782028241463131 1106 100-101 1.1844094555499964 1107 1 0.04126085636891119 1107 2 0.10832812931212032 1107 3 0.05732142216313818 1107 4 0.09672801802555853 1107 5 0.1456667338394766 1107 6 0.08742482574344734 1107 7 0.0550689121186565 1107 8 0.07255014139441585 1107 9 0.04430844423529834 1107 10-11 0.13748501052347706 1107 12-13 0.12307192901277375 1107 14-15 0.11440529211417783 1107 16-17 0.2473095115743007 1107 18-19 0.21625661604095825 1107 20-21 0.2250037556448632 1107 22-23 0.262945935029137 1107 24-25 0.10690197310892557 1107 26-27 0.19046902176183522 1107 28-29 -0.0037288312484307085 1107 30-31 -0.03997420465546497 1107 32-33 0.08381755930607682 1107 34-35 0.21858027664863755 1107 36-37 0.17981195174953513 1107 38-39 0.09766857259410955 1107 40-41 0.16436162269677368 1107 42-43 0.233843226556381 1107 44-45 0.6466739240230766 1107 46-47 0.6017614131785791 1107 48-49 0.5100030877420139 1107 50-51 0.634274480787127 1107 52-53 0.5180097958332865 1107 54-55 0.523845425295967 1107 56-57 0.5327075529888887 1107 58-59 0.8413274025019817 1107 60-61 0.7022742605720467 1107 62-63 0.6179172046055186 1107 64-65 0.624488448056141 1107 66-67 0.5119011757854466 1107 68-69 0.5418968683056278 1107 70-71 0.6111457963911704 1107 72-73 0.5248371233389584 1107 74-75 0.4849759816016963 1107 76-77 0.4058125591123627 1107 78-79 0.4633813160264033 1107 80-81 0.3459320825266303 1107 82-83 0.21667145056403925 1107 84-85 0.3661734564464041 1107 86-87 0.4546689472567138 1107 88-89 0.4792327733559887 1107 90-91 0.6640425293467551 1107 92-93 0.797608807056811 1107 94-95 0.8791124717345511 1107 96-97 1.0582624854079654 1107 98-99 1.0459622385886895 1107 100-101 1.4254010756617248 1108 1 0.12296756307561552 1108 2 0.13656887005285867 1108 3 0.06100010084181662 1108 4 0.16683562563316912 1108 5 0.2125085756813192 1108 6 0.1743367376553593 1108 7 0.20428062383036405 1108 8 0.2156682595125332 1108 9 0.12179843422529046 1108 10-11 0.34311564115410675 1108 12-13 0.357531388472232 1108 14-15 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-0.9696715532542939 2316 96-97 -1.1279406605090365 2316 98-99 -1.4364479577522289 2316 100-101 -1.6656129805631714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11074.0 3 2304.0 4 2189.0 5 2469.0 6 2806.0 7 3347.0 8 4187.0 9 4926.0 10 5921.0 11 7293.0 12 9246.0 13 11768.0 14 11960.0 15 13326.0 16 15872.0 17 19337.0 18 17760.0 19 13713.0 20 14119.0 21 15345.0 22 16896.0 23 18857.0 24 21938.0 25 24846.0 26 28735.0 27 34013.0 28 40251.0 29 47494.0 30 56552.0 31 68282.0 32 84103.0 33 106166.0 34 139395.0 35 191090.0 36 279665.0 37 397269.0 38 329871.0 39 47340.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.4839113970173 18.814452356118924 14.127025459817835 23.57461078704594 2 16.16746339653354 20.728604191589064 38.781923878385946 24.322008533491452 3 18.585222942208144 24.596507234016833 30.603069274482745 26.215200549292277 4 13.546198525635218 17.007641847185713 36.575689597873044 32.87047002930602 5 14.157783644152511 36.56997541539665 34.198345405173534 15.073895535277298 6 31.633245264219152 35.35882851550418 17.97927424438825 15.028651975888424 7 27.745197761199318 32.68730254943026 21.673204454735245 17.894295234635173 8 26.1514881489538 33.79232533373147 20.871505233586042 19.184681283728683 9 26.686698564773977 16.272377173642667 20.76204285712263 36.27888140446073 10-11 24.901607272100435 25.923163113382756 27.881879137577435 21.29335047693937 12-13 25.64371593680904 23.792959559513747 28.014687598076133 22.548636905601082 14-15 23.154731076849284 25.193153593282386 26.14120548615219 25.51090984371614 16-17 22.404974146161628 27.973947649522152 26.92282605613166 22.698252148184558 18-19 22.404813131428845 26.705207746038916 28.98195949643708 21.908019626095157 20-21 23.14562276941458 26.13465150626128 28.868388524150827 21.85133720017331 22-23 23.03945758663186 25.917355286000355 28.677801271795122 22.36538585557266 24-25 22.725603224598785 26.088851254397742 28.79600727967584 22.38953824132763 26-27 22.536719604944437 26.224380928600006 28.74644484989156 22.492454616563997 28-29 22.569266984789937 26.421850747454158 28.663598587876866 22.345283679879042 30-31 22.8838600704606 26.172352191441828 28.536187806067215 22.40759993203036 32-33 22.62292643538128 26.2330655938198 28.444915074159592 22.699092896639325 34-35 22.9244986727765 26.327956643401627 28.315078886078247 22.432465797743625 36-37 23.045707473212445 26.106618357705084 28.289890677867252 22.557783491215215 38-39 22.77015134100206 26.217246094771035 28.340902472943057 22.671700091283846 40-41 22.910577710684972 26.094073069363493 28.372855978101512 22.622493241850023 42-43 22.78037758985121 26.215661823005465 28.08769209226105 22.91626849488227 44-45 22.69007176000672 26.262672140629267 28.114711925029894 22.93254417433412 46-47 22.90696582346068 26.29876266861222 27.75130166656376 23.04296984136334 48-49 22.820270866922744 26.407135160408128 27.66218629045577 23.11040768221335 50-51 22.82366029179064 26.51630438897343 27.584138710336838 23.075896608899086 52-53 22.928324896347632 26.4899111064423 27.490543288881824 23.091220708328247 54-55 22.55474538899036 26.484418620056594 27.561275089665095 23.39956090128796 56-57 22.58672632956561 26.53541964253825 27.493020524141308 23.38483350375483 58-59 22.736545933824154 26.292056926201724 27.59744536327096 23.373951776703166 60-61 22.68063000502349 26.250524511687008 27.80400106379835 23.264844419491148 62-63 22.488437707208753 26.218343115340954 27.851576850481358 23.441642326968935 64-65 22.4127413216949 26.14535075501692 27.863217161957955 23.578690761330222 66-67 22.3558618966248 26.27583806203383 27.632980428989505 23.735319612351866 68-69 22.347537699953758 26.126052571917562 27.80401467057238 23.722395057556295 70-71 22.393899055158172 26.0723333510824 27.644356093549398 23.88941150021003 72-73 22.383886437606513 26.158461641125 27.482177786254592 23.97547413501389 74-75 22.329183449112687 26.288894220781035 27.37553541740907 24.006386912697206 76-77 22.40010939785283 26.053486654360984 27.484365144670615 24.062038803115566 78-79 22.319868131899323 26.1334041019797 27.552159354340173 23.994568411780804 80-81 22.461401916822872 26.071766839853794 27.471417500825563 23.995413742497774 82-83 22.46366457188117 25.966784039002565 27.544568209540376 24.02498317957589 84-85 22.433990260594395 25.92428302947372 27.44204334470796 24.199683365223926 86-87 22.416329659073302 25.72541106252314 27.673600802430947 24.184658475972608 88-89 22.568169515777363 25.593330771909635 27.526890217127974 24.311609495185028 90-91 22.549157644029915 25.575581319681163 27.647614912167572 24.22764612412135 92-93 22.595024038342086 25.43083140694522 27.57226351332964 24.401881041383053 94-95 22.639288470444797 25.29207087938497 27.577923235061714 24.490717415108527 96-97 22.68426420925835 24.99293589770896 27.65377142600205 24.66902846703064 98-99 22.63948096671275 24.983754178595653 27.599887545311997 24.776877309379604 100-101 23.08241907860915 24.513721687242672 27.832955471084965 24.570903763063217 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1957.0 1 1335.0 2 722.5 3 743.5 4 719.0 5 773.5 6 996.5 7 1510.5 8 2214.0 9 2547.5 10 2350.0 11 2166.0 12 2228.0 13 2381.0 14 2579.5 15 2800.5 16 3030.0 17 3258.0 18 3530.0 19 3799.0 20 4138.5 21 4630.0 22 5204.0 23 5962.5 24 6869.0 25 8318.5 26 10443.0 27 12738.0 28 14988.5 29 17762.5 30 21160.5 31 24972.0 32 29032.0 33 31896.0 34 34590.0 35 37870.5 36 41799.5 37 46027.5 38 49746.0 39 52645.0 40 54597.0 41 57202.5 42 60115.5 43 63594.0 44 67842.0 45 72380.0 46 76926.5 47 79842.5 48 82068.5 49 84038.0 50 84234.0 51 84216.0 52 84537.5 53 83715.5 54 81299.0 55 76189.0 56 68979.0 57 61224.0 58 55398.5 59 49142.5 60 41429.0 61 34521.5 62 28092.0 63 22194.0 64 16517.0 65 12176.0 66 9186.5 67 7204.5 68 5474.0 69 3923.0 70 2988.5 71 2295.0 72 1695.0 73 1289.0 74 1006.5 75 738.5 76 573.5 77 395.5 78 234.5 79 175.0 80 146.5 81 107.5 82 71.5 83 54.0 84 41.5 85 40.0 86 36.0 87 24.0 88 18.5 89 14.0 90 14.5 91 13.0 92 9.5 93 7.0 94 4.5 95 4.5 96 4.0 97 4.5 98 4.0 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26992149794884174 2 0.16430004222972974 3 0.19295593026061778 4 0.15986969111969113 5 0.2335831925675676 6 0.1919661709700772 7 0.23843772623069495 8 0.24536604126447875 9 0.14643724360521235 10-11 0.2036311911800193 12-13 0.2088627760014479 14-15 0.169437364261583 16-17 0.282222791988417 18-19 0.22870509320704635 20-21 0.1956188540661197 22-23 0.2410063872466216 24-25 0.22978911528716214 26-27 0.338874728523166 28-29 0.3117741765202703 30-31 0.2864410518219112 32-33 0.18843131636100385 34-35 0.35537071669884174 36-37 0.395267442386583 38-39 0.37665054144546334 40-41 0.34141982384169883 42-43 0.3723380188223938 44-45 0.3810808925555019 46-47 0.38588829482384174 48-49 0.4047172870415058 50-51 0.3974119208494209 52-53 0.3965635557432432 54-55 0.3915676278957529 56-57 0.37005214617519305 58-59 0.34766473365106176 60-61 0.31415431195704635 62-63 0.38027965884411197 64-65 0.4114099451013513 66-67 0.4162880444618726 68-69 0.420741961269305 70-71 0.42137823509893824 72-73 0.413577989261583 74-75 0.4080164846766409 76-77 0.3931229639237452 78-79 0.36750705085666024 80-81 0.37808804898648646 82-83 0.387702853523166 84-85 0.3889282697876448 86-87 0.38414443321669883 88-89 0.3921332046332046 90-91 0.412658927063224 92-93 0.4123761386944981 94-95 0.4207655269666989 96-97 0.4210247496380309 98-99 0.41838539152992277 100-101 0.3913555366192085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2121728.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.85659763149067 #Duplication Level Percentage of deduplicated Percentage of total 1 77.26215934390022 45.47387824644052 2 12.630135156319067 14.867335658536318 3 4.173122228730288 7.368473276502244 4 1.8931515980619047 4.456978474501723 5 1.0495350453147487 3.0886030931119244 6 0.6704275375484332 2.367545029113554 7 0.4624431879704416 1.9052482849260062 8 0.32708396913499443 1.5400839650471425 9 0.22822752377378028 1.208942598166637 >10 1.230347575925899 12.474549621870953 >50 0.05240546597562916 2.0829890550985857 >100 0.01983801164886174 2.037135123923817 >500 8.023969254655217E-4 0.2897809019028799 >1k 1.6047938509307927E-4 0.22909503831986378 >5k 1.6047938509307927E-4 0.609361632537869 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7356 0.34669854005791506 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5547 0.2614378468870656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3671 0.17301935026544402 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.713139478764479E-5 0.0 12-13 0.0 0.0 0.0 9.426278957528958E-5 0.0 14-15 0.0 0.0 0.0 1.6495988175675675E-4 0.0 16-17 0.0 0.0 0.0 1.8852557915057915E-4 0.0 18-19 0.0 0.0 0.0 2.1209127654440155E-4 0.0 20-21 0.0 0.0 0.0 4.241825530888031E-4 0.0 22-23 0.0 0.0 0.0 7.77668013996139E-4 0.0 24-25 0.0 0.0 0.0 0.0016260331201737453 0.0 26-27 0.0 0.0 0.0 0.0033227633325289578 0.0 28-29 0.0 0.0 0.0 0.011900677183880309 0.0 30-31 0.0 0.0 0.0 0.035890557130791506 0.0 32-33 0.0 0.0 0.0 0.08610905827702703 0.0 34-35 0.0 0.0 0.0 0.1648891846645753 0.0 36-37 0.0 0.0 0.0 0.29110705990588803 0.0 38-39 0.0 0.0 0.0 0.4734112949444981 0.0 40-41 0.0 2.3565697393822394E-5 0.0 0.6758642012548263 0.0 42-43 0.0 4.713139478764479E-5 0.0 0.8806972430019305 0.0 44-45 0.0 4.713139478764479E-5 0.0 1.1305878981660231 0.0 46-47 0.0 4.713139478764479E-5 0.0 1.4209644214527026 0.0 48-49 0.0 4.713139478764479E-5 0.0 1.728190418375965 0.0 50-51 0.0 4.713139478764479E-5 0.0 2.055140904017857 0.0 52-53 0.0 4.713139478764479E-5 0.0 2.379946911196911 0.0 54-55 0.0 4.713139478764479E-5 0.0 2.739842241795367 2.3565697393822394E-5 56-57 0.0 4.713139478764479E-5 0.0 3.153679453728282 4.713139478764479E-5 58-59 0.0 4.713139478764479E-5 0.0 3.605221781491313 4.713139478764479E-5 60-61 0.0 4.713139478764479E-5 0.0 4.0882478809724905 4.713139478764479E-5 62-63 0.0 4.713139478764479E-5 0.0 4.575185886221043 4.713139478764479E-5 64-65 0.0 4.713139478764479E-5 0.0 5.0914396190275095 4.713139478764479E-5 66-67 0.0 4.713139478764479E-5 0.0 5.654989706503379 4.713139478764479E-5 68-69 0.0 4.713139478764479E-5 0.0 6.285183586208976 4.713139478764479E-5 70-71 0.0 4.713139478764479E-5 0.0 6.96590232112693 4.713139478764479E-5 72-73 0.0 4.713139478764479E-5 0.0 7.657178488477316 7.069709218146718E-5 74-75 0.0 4.713139478764479E-5 0.0 8.357079701073841 9.426278957528958E-5 76-77 0.0 4.713139478764479E-5 0.0 9.1041594398528 9.426278957528958E-5 78-79 0.0 4.713139478764479E-5 0.0 9.920404500482626 9.426278957528958E-5 80-81 0.0 4.713139478764479E-5 0.0 10.792971577883687 1.1782848696911196E-4 82-83 0.0 4.713139478764479E-5 0.0 11.68304796844836 1.4139418436293435E-4 84-85 0.0 4.713139478764479E-5 0.0 12.584860076315156 1.4139418436293435E-4 86-87 0.0 4.713139478764479E-5 0.0 13.527770760436777 1.4139418436293435E-4 88-89 0.0 4.713139478764479E-5 0.0 14.510177553390443 1.4139418436293435E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4355 0.0 53.462357 1 GTATCAA 7455 0.0 39.963184 1 TCAACGC 9520 0.0 31.296469 4 ATCAACG 9505 0.0 31.196115 3 TATCAAC 9545 0.0 31.015678 2 CAACGCA 9695 0.0 30.633677 5 AACGCAG 10020 0.0 29.54328 6 ACGCAGA 11345 0.0 25.96743 7 CGCAGAG 11485 0.0 25.403055 8 GTACATG 7900 0.0 24.179045 1 GCAGAGT 12195 0.0 23.37891 9 TACATGG 8010 0.0 23.336533 2 ACATGGG 8195 0.0 22.23079 3 GAGTACT 6870 0.0 21.831451 12-13 CAGAGTA 11790 0.0 20.972267 10-11 CATGGGG 4995 0.0 20.230972 4 GTGGTAT 1985 0.0 19.868166 1 GTACTTT 7480 0.0 19.607374 14-15 AGAGTAC 11235 0.0 19.241402 10-11 AGTACTT 7210 0.0 19.156227 12-13 >>END_MODULE