FastQCFastQC Report
Thu 26 May 2016
SRR938798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938798_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121728
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66160.3118213079150579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57950.2731264327944015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.17160540842181465No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT22470.10590424408783783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42600.053.5920221
GTATCAA73900.040.8693581
ATCAACG95050.031.552913
TCAACGC95550.031.38784
TATCAAC96450.031.1441122
CAACGCA98900.030.3246145
AACGCAG101750.029.4731436
ACGCAGA114700.025.7306217
CGCAGAG116950.025.0733038
CGTATAC1201.7227152E-423.727093
GCAGAGT126000.022.9711449
GTACATG72600.022.3401581
TACATGG74300.021.3958952
GAGTACT73600.021.33947612-13
ACATGGG75050.020.549783
CAGAGTA120450.020.30745110-11
GTGGTAT20150.020.2998071
GTACTTT78450.019.83875714-15
AGAGTAC115050.019.59027510-11
AGTACTT76050.019.40415412-13
Produced by FastQC (version 0.11.5)