##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938798_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2121728 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.036089451616796 33.0 31.0 34.0 30.0 34.0 2 32.24071322997104 34.0 31.0 34.0 30.0 34.0 3 32.320986478945464 34.0 31.0 34.0 30.0 34.0 4 35.95344455085666 37.0 35.0 37.0 35.0 37.0 5 35.84934779575893 37.0 35.0 37.0 35.0 37.0 6 35.86755842407698 37.0 35.0 37.0 35.0 37.0 7 35.78322763332529 37.0 35.0 37.0 33.0 37.0 8 35.771851999879345 37.0 35.0 37.0 33.0 37.0 9 37.41230261371863 39.0 37.0 39.0 34.0 39.0 10-11 37.34477039469715 39.0 37.0 39.0 34.0 39.0 12-13 37.26519091985401 39.0 37.0 39.0 33.0 39.0 14-15 38.492872554823236 40.0 38.0 41.0 33.5 41.0 16-17 38.416458660111005 40.0 38.0 41.0 33.0 41.0 18-19 38.52903930192748 40.0 38.0 41.0 34.0 41.0 20-21 38.5182283025911 40.0 38.0 41.0 34.0 41.0 22-23 38.438659196654804 40.0 38.0 41.0 34.0 41.0 24-25 38.355013696383324 40.0 38.0 41.0 34.0 41.0 26-27 38.170565454195824 40.0 38.0 41.0 33.0 41.0 28-29 37.99309525066361 40.0 37.0 41.0 33.0 41.0 30-31 37.76260529153596 40.0 37.0 41.0 32.5 41.0 32-33 37.612864608470076 40.0 37.0 41.0 32.0 41.0 34-35 37.58726872624578 40.0 37.0 41.0 32.0 41.0 36-37 37.476294558020626 40.0 37.0 41.0 32.0 41.0 38-39 37.34167833954211 40.0 36.0 41.0 31.5 41.0 40-41 37.16362276408663 40.0 36.0 41.0 31.0 41.0 42-43 36.964266390413854 40.0 36.0 41.0 31.0 41.0 44-45 36.71354504441663 39.5 35.5 41.0 30.5 41.0 46-47 36.39817544944498 39.0 35.0 41.0 30.0 41.0 48-49 36.08360685252775 39.0 35.0 41.0 29.5 41.0 50-51 35.834927945523646 39.0 35.0 41.0 28.5 41.0 52-53 35.59764941594776 39.0 35.0 40.0 28.0 41.0 54-55 35.30540436851472 38.0 34.5 40.0 27.5 41.0 56-57 35.01370416000543 38.0 34.0 40.0 27.0 41.0 58-59 34.68546180283241 38.0 34.0 40.0 26.0 41.0 60-61 34.29909771657819 37.0 33.5 40.0 26.0 41.0 62-63 33.909305292667106 36.5 33.0 39.5 25.5 41.0 64-65 33.495020096826735 36.0 33.0 39.0 24.5 40.5 66-67 33.07886331329935 35.5 32.0 39.0 23.0 40.0 68-69 32.734518750754106 35.0 32.0 38.5 22.5 40.0 70-71 32.313771133717424 35.0 32.0 38.0 22.0 40.0 72-73 31.83931776363417 35.0 31.0 37.0 20.0 39.0 74-75 31.353630154289334 35.0 31.0 36.5 18.5 39.0 76-77 29.406019763136463 32.5 28.5 35.0 15.5 36.5 78-79 30.373019067477074 34.0 30.0 35.0 15.5 37.0 80-81 30.452469166641528 34.0 30.0 35.0 16.0 37.0 82-83 30.161107361546815 34.0 30.0 35.0 11.5 37.0 84-85 29.792049687801644 34.0 30.0 35.0 7.0 36.0 86-87 29.24631456058458 34.0 29.0 35.0 2.0 36.0 88-89 28.677080191240346 34.0 29.0 35.0 2.0 35.0 90-91 28.18068739254042 33.5 28.0 35.0 2.0 35.0 92-93 27.899512567115107 33.0 27.0 35.0 2.0 35.0 94-95 27.58672176640927 33.0 27.0 35.0 2.0 35.0 96-97 27.013223419778598 33.0 26.5 35.0 2.0 35.0 98-99 26.214709896838805 33.0 25.0 35.0 2.0 35.0 100-101 22.71001678820282 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.188860494069786 1101 2 0.24562519586734055 1101 3 0.07482374076246145 1101 4 0.1530847680473144 1101 5 0.08419153082469677 1101 6 0.180471926902527 1101 7 0.2768358757233571 1101 8 0.18212250517188977 1101 9 0.20495045410365265 1101 10-11 0.2605895876675177 1101 12-13 0.2858597890505621 1101 14-15 0.3868846997385518 1101 16-17 0.28229781744910554 1101 18-19 0.31150366295187837 1101 20-21 0.26793265599134486 1101 22-23 0.3119053015155302 1101 24-25 0.29995545137904145 1101 26-27 0.13519746807455846 1101 28-29 0.2510121244882981 1101 30-31 0.23877979051555798 1101 32-33 0.3103371653007798 1101 34-35 0.3336775681282731 1101 36-37 0.26489817891854983 1101 38-39 0.3752597580052779 1101 40-41 0.43036269718896136 1101 42-43 0.592009029332722 1101 44-45 0.5880512943550187 1101 46-47 0.6148837050776876 1101 48-49 0.54231522530538 1101 50-51 0.49588216608078994 1101 52-53 0.39033949160060644 1101 54-55 0.5588845174172761 1101 56-57 0.5189183429319044 1101 58-59 0.43321267383613105 1101 60-61 0.45491700059695717 1101 62-63 0.48970942627215663 1101 64-65 0.34289818785071446 1101 66-67 0.3512736588452299 1101 68-69 0.373317743719916 1101 70-71 0.513259587632831 1101 72-73 0.4014758611693061 1101 74-75 0.10855308871328262 1101 76-77 0.11842767363608075 1101 78-79 0.1559786806538348 1101 80-81 0.21285186872340844 1101 82-83 0.31231089760894903 1101 84-85 0.3512023724109383 1101 86-87 0.4091596329084517 1101 88-89 0.4736303014959624 1101 90-91 0.3262856393085052 1101 92-93 0.4166712323840045 1101 94-95 0.3981002095059125 1101 96-97 0.1480454088884784 1101 98-99 -0.01132324835923626 1101 100-101 0.5297454603749863 1102 1 0.2519623453320037 1102 2 0.13304132748168485 1102 3 0.09191930236134027 1102 4 0.11885015019590384 1102 5 -0.0039031654468502097 1102 6 -0.07417148782668193 1102 7 0.1106307890596625 1102 8 0.1639253994166836 1102 9 0.14889954277050776 1102 10-11 0.2310571984485179 1102 12-13 0.2106160808165498 1102 14-15 0.28012466935477676 1102 16-17 0.3567371148927023 1102 18-19 0.36904060156746255 1102 20-21 0.32467059061047365 1102 22-23 0.2585013162037484 1102 24-25 0.3601194911047969 1102 26-27 0.35907658561849587 1102 28-29 0.33235469631034675 1102 30-31 0.39443357883981633 1102 32-33 0.3308140156919066 1102 34-35 0.3061914701970565 1102 36-37 0.21897239040973915 1102 38-39 0.26644298319872917 1102 40-41 0.2219519365635918 1102 42-43 0.4048526160700803 1102 44-45 0.26572214844343733 1102 46-47 0.33627918177788985 1102 48-49 0.3641665307817803 1102 50-51 0.2082895086511698 1102 52-53 0.05175728274023328 1102 54-55 0.21817046977356824 1102 56-57 0.16172955378701914 1102 58-59 0.31006489125845604 1102 60-61 0.15283689882426899 1102 62-63 0.039655466468431655 1102 64-65 0.2620853616858696 1102 66-67 0.3927828175937478 1102 68-69 0.29939137079676215 1102 70-71 0.2830564435701248 1102 72-73 0.34924911512930024 1102 74-75 0.3561083167105714 1102 76-77 0.25557809136498477 1102 78-79 0.19505177834546927 1102 80-81 0.37082144697665953 1102 82-83 0.47886514301155003 1102 84-85 0.5166657670941603 1102 86-87 0.5815858968546728 1102 88-89 0.6069081330880302 1102 90-91 0.7426522689606614 1102 92-93 0.7412503254892364 1102 94-95 0.759756572425303 1102 96-97 0.7860629562177515 1102 98-99 1.022028500113823 1102 100-101 1.7532408156986712 1103 1 0.25487194937959856 1103 2 0.2358535366595902 1103 3 0.143643346934617 1103 4 0.061589170790085745 1103 5 0.09474933874545854 1103 6 0.11383098035754102 1103 7 0.173121710525308 1103 8 0.11544102320645777 1103 9 0.12685538995873458 1103 10-11 0.2674219520055985 1103 12-13 0.18910245577573193 1103 14-15 0.3217567973726432 1103 16-17 0.226243292062847 1103 18-19 0.13970346346992102 1103 20-21 0.207633833640692 1103 22-23 0.2317511572337594 1103 24-25 0.3601053656704707 1103 26-27 0.40207111177727484 1103 28-29 0.32296893375936975 1103 30-31 0.3596000223638569 1103 32-33 0.355130950887002 1103 34-35 0.42127067739293267 1103 36-37 0.4261987507302578 1103 38-39 0.35416381376484907 1103 40-41 0.518208437500455 1103 42-43 0.4661446412971628 1103 44-45 0.3623727254556499 1103 46-47 0.41701863413950946 1103 48-49 0.46505662148354787 1103 50-51 0.40297283642458126 1103 52-53 0.4106467834696943 1103 54-55 0.42744960883776884 1103 56-57 0.29276002540937185 1103 58-59 0.4222534460311138 1103 60-61 0.42476198599585757 1103 62-63 0.2494902059495132 1103 64-65 0.5057757067396196 1103 66-67 0.39775037971093496 1103 68-69 0.2612908367847169 1103 70-71 0.2741530646014425 1103 72-73 0.2989309044074808 1103 74-75 0.36199579773834856 1103 76-77 0.43243912322325073 1103 78-79 0.2882260859696508 1103 80-81 0.35404019557147137 1103 82-83 0.43671708350486327 1103 84-85 0.5173407274311188 1103 86-87 0.5113388171116533 1103 88-89 0.33593863154871073 1103 90-91 0.24874221454713208 1103 92-93 0.3288204847558589 1103 94-95 0.4491687589554303 1103 96-97 0.48549384246868854 1103 98-99 0.7542414589285293 1103 100-101 1.3451730267976387 1104 1 0.08569371604834686 1104 2 0.05681574609884166 1104 3 0.022263500832998773 1104 4 -0.010656240578200027 1104 5 -0.12346146671855251 1104 6 -0.07629243117177253 1104 7 -0.0058234987652099335 1104 8 -0.0342090590377353 1104 9 -0.016255298954973796 1104 10-11 0.019918223811210112 1104 12-13 5.719525675260684E-4 1104 14-15 -0.025501045177477977 1104 16-17 0.051475725578931986 1104 18-19 0.1407053830810625 1104 20-21 0.0846180370484646 1104 22-23 0.04116507138655834 1104 24-25 0.023079261696828723 1104 26-27 0.05664830425373424 1104 28-29 0.040420758434109416 1104 30-31 -0.17122049132529327 1104 32-33 -0.006879450235857121 1104 34-35 -0.09824424779838381 1104 36-37 0.017806409405622503 1104 38-39 0.04647274655804523 1104 40-41 0.11695436734705567 1104 42-43 0.15591466617345162 1104 44-45 0.18531232232571426 1104 46-47 0.16040597630758668 1104 48-49 0.21868069094316667 1104 50-51 0.11695471973180105 1104 52-53 -0.005153683706062395 1104 54-55 0.02811874634925715 1104 56-57 -0.01151632434549299 1104 58-59 0.1837009808749741 1104 60-61 0.09772978650851627 1104 62-63 0.06235236636013752 1104 64-65 -0.0722923472229624 1104 66-67 -0.2675227338760777 1104 68-69 -0.2462429350760118 1104 70-71 -0.22203335277487923 1104 72-73 0.016668362023704475 1104 74-75 -0.012427570609574445 1104 76-77 -0.09548515133056767 1104 78-79 -0.3677083850536853 1104 80-81 -0.08295952622065172 1104 82-83 0.12309431807215532 1104 84-85 0.07571205814417681 1104 86-87 0.17066306201162362 1104 88-89 0.06051840191467761 1104 90-91 0.30944304316110305 1104 92-93 0.3738559359572946 1104 94-95 0.321950956064434 1104 96-97 0.23873563713456747 1104 98-99 0.6480324113484848 1104 100-101 1.4834146137937516 1105 1 0.02036912471117347 1105 2 0.04058737431042658 1105 3 0.07037338902677703 1105 4 -0.01920361100241763 1105 5 -0.04781397193873005 1105 6 -0.09388705285462606 1105 7 -0.06882487343750654 1105 8 0.05126555573419722 1105 9 0.0812690389362416 1105 10-11 0.09892602864461963 1105 12-13 0.06337050673732847 1105 14-15 -0.032829785343466256 1105 16-17 0.041672620809059424 1105 18-19 -0.005779163977045698 1105 20-21 -0.008435941203984498 1105 22-23 -0.11701375073850073 1105 24-25 -0.162451532760862 1105 26-27 -0.02743604540152944 1105 28-29 -0.002029845895876292 1105 30-31 -0.04214641130749186 1105 32-33 -0.04017171867732827 1105 34-35 0.001234656023953562 1105 36-37 -0.17179115237779996 1105 38-39 -0.08645867894230008 1105 40-41 -0.07897672558228663 1105 42-43 0.014428813643299065 1105 44-45 0.017806147226743008 1105 46-47 -0.2014520916803093 1105 48-49 -0.2214922687360641 1105 50-51 -0.16781323289879424 1105 52-53 -0.11436489860998478 1105 54-55 -0.09027197425992739 1105 56-57 -0.07980332423418446 1105 58-59 -0.11683723326205353 1105 60-61 5.82521751276488E-4 1105 62-63 -0.189117285569381 1105 64-65 -0.14418128899845328 1105 66-67 -0.14385890783459843 1105 68-69 -0.07989028735791948 1105 70-71 -0.10088088160851072 1105 72-73 -0.03985914610447949 1105 74-75 -0.0767346449477273 1105 76-77 0.11202150634917274 1105 78-79 -0.15489049350825113 1105 80-81 -0.1031100049202891 1105 82-83 -0.1673962002400735 1105 84-85 -0.22971274154496868 1105 86-87 -0.2379703990007478 1105 88-89 -0.22072375187971716 1105 90-91 -0.34857563954363613 1105 92-93 -0.3554119275552061 1105 94-95 -0.02681130393414577 1105 96-97 -0.045763140137786706 1105 98-99 0.13467437079030375 1105 100-101 0.944315783300933 1106 1 0.043131375347577716 1106 2 0.027689074658226787 1106 3 0.03025204156934791 1106 4 0.06589589603181167 1106 5 -0.03670568635234872 1106 6 0.007614625242169382 1106 7 0.07230743793884642 1106 8 0.04943382102977978 1106 9 0.011071084439556955 1106 10-11 0.02995949521911001 1106 12-13 0.02389847810769652 1106 14-15 -0.027777945794426273 1106 16-17 0.04964291836329693 1106 18-19 -0.10258594576618663 1106 20-21 0.01484285067228086 1106 22-23 -0.016329267372455547 1106 24-25 0.02426123852261952 1106 26-27 0.11899246926427054 1106 28-29 0.17050883509620718 1106 30-31 0.06983774279662924 1106 32-33 -0.08173209705040563 1106 34-35 -0.0369069477968651 1106 36-37 -0.15009542695523237 1106 38-39 0.06398627497957676 1106 40-41 0.10727248305004622 1106 42-43 -0.03262544437831849 1106 44-45 -0.010416871440263265 1106 46-47 -0.1564686342446322 1106 48-49 -0.0739459058486247 1106 50-51 -0.13185302246784403 1106 52-53 -0.1400773944048055 1106 54-55 -0.008992554587386792 1106 56-57 -0.10418075959368167 1106 58-59 0.05225883281284638 1106 60-61 0.028382042824837583 1106 62-63 2.603087258847836E-4 1106 64-65 -0.08522737772170075 1106 66-67 -0.015419604079212945 1106 68-69 -0.09512825393372992 1106 70-71 -0.023464263284807885 1106 72-73 0.014554169004217243 1106 74-75 0.06342948272007476 1106 76-77 -0.07135626277543494 1106 78-79 -0.03806111635103093 1106 80-81 -0.06002814770288367 1106 82-83 0.13223712170286106 1106 84-85 0.22068307473588078 1106 86-87 0.28215285568587234 1106 88-89 0.41324221202597045 1106 90-91 0.42098703545126526 1106 92-93 0.5847196182805448 1106 94-95 0.7477661255977068 1106 96-97 0.7576975537227852 1106 98-99 0.6435257684979199 1106 100-101 1.355086595277271 1107 1 -0.03938207385581194 1107 2 -0.032240058385426096 1107 3 0.018447779206425707 1107 4 0.035586047076712646 1107 5 0.024395079217690352 1107 6 -0.015880098531887654 1107 7 0.01602510812300295 1107 8 0.01928950073182989 1107 9 0.04877799526099125 1107 10-11 -0.00402560720174705 1107 12-13 0.1038669242074306 1107 14-15 -0.015024480038164256 1107 16-17 -0.009412016459791062 1107 18-19 0.04621526466211634 1107 20-21 -0.02283948948910819 1107 22-23 0.013028791804046591 1107 24-25 -4.043745503423679E-5 1107 26-27 -0.11707338010465662 1107 28-29 0.021436054214767353 1107 30-31 0.11316745519059168 1107 32-33 -0.06127637596619451 1107 34-35 -0.148276697434774 1107 36-37 -0.08615222397571642 1107 38-39 -0.12626822533079718 1107 40-41 -0.33458298974118605 1107 42-43 -0.2336742236044742 1107 44-45 -0.1576778894415085 1107 46-47 -0.1101374217472113 1107 48-49 -0.03369657830879902 1107 50-51 -0.15792637235404072 1107 52-53 -0.09814045047763642 1107 54-55 -0.06710604517294172 1107 56-57 -0.19531851047305082 1107 58-59 -0.2311637308122343 1107 60-61 -0.21440582185548607 1107 62-63 -0.15548793253212523 1107 64-65 -0.21158652318000293 1107 66-67 -0.26230300983134214 1107 68-69 -0.21243763733948384 1107 70-71 -0.31756730487388296 1107 72-73 -0.3788208051319266 1107 74-75 -0.12353901510736165 1107 76-77 -0.28958898572184566 1107 78-79 -0.048012233669464166 1107 80-81 0.046166245013857576 1107 82-83 0.052899753608510736 1107 84-85 -0.10091453544412943 1107 86-87 -0.058258120933206925 1107 88-89 -0.10806701619666725 1107 90-91 0.07622317242623211 1107 92-93 0.26887024935854953 1107 94-95 0.23089772088014016 1107 96-97 0.5594601752828936 1107 98-99 0.6403445146183451 1107 100-101 1.1828915301003633 1108 1 0.07234215036841363 1108 2 0.007211893566520189 1108 3 0.020349681108328355 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-0.305216042910903 2316 84-85 -0.4299389665016946 2316 86-87 -0.7467426144628782 2316 88-89 -0.9620775025866699 2316 90-91 -1.4427332086536424 2316 92-93 -1.2155563459114163 2316 94-95 -1.1064182095080781 2316 96-97 -1.3399904048152749 2316 98-99 -1.4220718173763025 2316 100-101 -1.5885191124699212 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 23.0 5 61.0 6 165.0 7 438.0 8 916.0 9 1704.0 10 2814.0 11 4166.0 12 5709.0 13 7702.0 14 9058.0 15 11247.0 16 14258.0 17 19011.0 18 18179.0 19 13464.0 20 12931.0 21 13729.0 22 14793.0 23 16126.0 24 18715.0 25 22046.0 26 26310.0 27 31564.0 28 38610.0 29 47172.0 30 56725.0 31 68822.0 32 84691.0 33 106656.0 34 142434.0 35 198193.0 36 298198.0 37 446554.0 38 340794.0 39 27739.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.08693417533278 19.595666423854887 14.661674380900822 23.65572501991151 2 16.371372767857142 21.299337144063706 37.97084263392857 24.35844745415058 3 18.50420977618243 24.934063178692085 30.825958841095556 25.735768204029924 4 13.855121862934364 17.587079965009654 36.07271997164575 32.485078200410236 5 14.063798681935808 36.916452041613454 34.10598352490475 14.913765751545984 6 31.618219241937982 35.921055670429624 17.5567848939998 14.90394019363259 7 27.67833577159749 32.87933231780888 21.38751998371139 18.054811926882238 8 26.2451171875 34.307507842664094 20.421750573117762 19.025624396718147 9 26.429212415540544 16.337579557794403 20.547497134411195 36.68571089225386 10-11 24.861952144666986 26.214646740769787 27.647229051037648 21.27617206352558 12-13 25.53489419944498 23.952858236305502 27.86893513211873 22.64331243213079 14-15 23.02314434272442 25.45981388754826 25.847799529440152 25.66924224028716 16-17 22.421491586216973 28.240098884485903 26.704985583273533 22.633423946023587 18-19 22.318789213320464 26.914241599300194 28.81382533482143 21.953143852557915 20-21 23.07246734736969 26.196996033421815 28.76334761100386 21.96718900820463 22-23 22.965997526544403 26.044903022442085 28.569236961570947 22.41986248944257 24-25 22.749813417755465 26.29901186231821 28.622859253006467 22.328315466919857 26-27 22.577168036400984 26.443075626584516 28.546458714022382 22.433297622992118 28-29 22.434190433458014 26.57958513061052 28.585803646838805 22.400420789092664 30-31 22.79408576405647 26.286569249215734 28.52531050162886 22.39403448509894 32-33 22.57344013935811 26.48402622767857 28.305348282154924 22.637185350808398 34-35 22.902987320555614 26.52433901376024 28.242442496371222 22.330231169312924 36-37 23.024897737437115 26.268403431261056 28.217207922682313 22.489490908619516 38-39 22.734093775388104 26.31635140540053 28.282726627154226 22.666828192057146 40-41 22.87180739292458 26.215059612300863 28.298096384610815 22.615036610163738 42-43 22.769324749400262 26.31767983991029 27.995760667678343 22.9172347430111 44-45 22.65234917676004 26.33929528821352 28.06913023958975 22.93922529543668 46-47 22.858041725679463 26.391739701137993 27.700633685485563 23.04958488769698 48-49 22.84677310836839 26.511039412620153 27.506140731448724 23.136046747562737 50-51 22.783421241281275 26.614781260737125 27.438991915548044 23.162805582433556 52-53 22.847980513996138 26.64738364201255 27.390504343629345 23.11413150036197 54-55 22.51568061504585 26.617973651665057 27.385084233228763 23.481261500060327 56-57 22.55776028656266 26.76005090257812 27.210797945044067 23.47139086581515 58-59 22.608258967497093 26.559420243406525 27.35526116100255 23.477059628093837 60-61 22.622524771724954 26.621797986777416 27.454207128054136 23.301470113443497 62-63 22.401639999085642 26.562484903086357 27.516880853331173 23.51899424449683 64-65 22.267419335681772 26.48843329905255 27.45450097893476 23.789646386330922 66-67 22.16520829852284 26.652963214432084 27.281684893027997 23.90014359401708 68-69 22.17847107208202 26.500832814101 27.33680472748079 23.983891386336193 70-71 22.224165084425348 26.37776358074427 27.183946658534925 24.21412467629546 72-73 22.10907807221284 26.48968199505309 26.982110807794403 24.419129124939673 74-75 22.057327101279668 26.597230830284314 26.851317257374795 24.49412481106122 76-77 21.998314542096832 26.370908260913033 27.043582338438437 24.587194858551694 78-79 21.863590282838274 26.43014374119788 27.087069710894053 24.619196265069792 80-81 21.93972103528205 26.388295811570146 26.974405453734786 24.697577699413017 82-83 21.920698758122587 26.307378917070075 26.951035180635213 24.820887144172122 84-85 21.802582611908782 26.21276148497828 26.848234080900095 25.13642182221284 86-87 21.67839558245437 26.106320781600605 27.015193805471732 25.20008983047329 88-89 21.730420072048023 25.92511053843058 26.937254276935125 25.40721511258627 90-91 21.663348545593728 25.871834734860993 26.984580199333287 25.480236520211996 92-93 21.489005958616076 25.814723213622926 26.976517844623523 25.719752983137482 94-95 21.36467957740919 25.686382496477506 27.03712527775205 25.91181264836125 96-97 21.36540689858 25.429502812921456 27.09234672800665 26.112743560491893 98-99 21.19786325108591 25.53343312620656 27.012628385919403 26.256075236788128 100-101 21.318574158514238 25.11294553422395 27.340560684938573 26.22791962232324 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 558.0 1 408.5 2 322.5 3 419.5 4 438.0 5 461.0 6 672.5 7 1186.5 8 1735.0 9 2013.5 10 1882.0 11 1692.5 12 1786.5 13 1982.0 14 2205.0 15 2398.0 16 2567.5 17 2808.0 18 3088.5 19 3389.5 20 3774.0 21 4272.0 22 4859.5 23 5659.5 24 6584.0 25 7964.0 26 10039.5 27 12584.0 28 14889.0 29 17527.0 30 21089.5 31 24890.0 32 28804.5 33 31928.5 34 34640.0 35 37764.5 36 41829.5 37 45872.5 38 49653.0 39 52473.0 40 54426.5 41 57533.5 42 60512.5 43 64024.5 44 68710.5 45 73021.0 46 77341.0 47 80872.0 48 83432.0 49 85177.0 50 85794.5 51 85851.0 52 85547.0 53 84908.0 54 82761.0 55 77513.5 56 69897.0 57 62022.0 58 56168.5 59 49582.0 60 41627.0 61 34699.5 62 28036.5 63 21778.0 64 16220.5 65 11862.0 66 8637.5 67 6768.5 68 5077.5 69 3545.5 70 2608.5 71 1933.0 72 1445.5 73 1063.5 74 793.0 75 584.0 76 410.5 77 245.0 78 149.0 79 93.0 80 59.0 81 40.0 82 33.0 83 28.5 84 19.5 85 13.5 86 11.5 87 7.5 88 2.5 89 2.0 90 2.5 91 1.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22915284145752896 2 0.0 3 0.0 4 0.0 5 0.027666128740347493 6 0.0038176429777992274 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0028514493846525098 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.477482504826255E-4 26-27 0.0012725476592664092 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006810486546814672 36-37 0.016519553873069498 38-39 0.01350314460666023 40-41 0.00876643943050193 42-43 0.007894508626930502 44-45 0.0037940772804054054 46-47 6.127081322393822E-4 48-49 0.0016260331201737453 50-51 4.713139478764479E-5 52-53 0.0 54-55 0.0 56-57 0.001319679054054054 58-59 4.7131394787644786E-4 60-61 0.002592226713320463 62-63 9.190621983590734E-4 64-65 0.0016024674227799226 66-67 0.005726464466698842 68-69 2.827883687258687E-4 70-71 7.069709218146718E-5 72-73 0.0 74-75 4.241825530888031E-4 76-77 0.05825440395752896 78-79 0.1376236727799228 80-81 0.0012254162644787644 82-83 9.897592905405406E-4 84-85 0.0 86-87 0.10274644063706564 88-89 0.3442948389237452 90-91 0.3809159326737452 92-93 4.948796452702703E-4 94-95 3.063540661196911E-4 96-97 7.069709218146718E-5 98-99 0.0 100-101 9.190621983590734E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2121728.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.95734585291166 #Duplication Level Percentage of deduplicated Percentage of total 1 76.08447641115632 43.335698369879296 2 13.0993713879571 14.922108531992162 3 4.424937650230844 7.560981123653053 4 2.0366796595105057 4.640154710333209 5 1.1461342836545065 3.2640383393994443 6 0.7137000249488908 2.4390275493747398 7 0.456692917684975 1.8208411522810906 8 0.3469465892168947 1.5808925499611794 9 0.26473550587847955 1.3570768591079478 >10 1.3441415814772661 13.265139348400986 >50 0.058738888640433654 2.27895866766534 >100 0.02220340240540303 2.2824232232284576 >500 7.450183431300405E-4 0.2862427084516755 >1k 3.31119263613331E-4 0.38124831832319833 >5k 1.655596318066655E-4 0.5851685479482086 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6616 0.3118213079150579 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5795 0.2731264327944015 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3641 0.17160540842181465 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2247 0.10590424408783783 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.713139478764479E-5 0.0 12-13 0.0 0.0 0.0 9.426278957528958E-5 0.0 14-15 0.0 0.0 0.0 1.6495988175675675E-4 0.0 16-17 0.0 0.0 0.0 1.8852557915057915E-4 4.713139478764479E-5 18-19 0.0 0.0 0.0 2.1209127654440155E-4 4.713139478764479E-5 20-21 0.0 0.0 0.0 4.241825530888031E-4 4.713139478764479E-5 22-23 0.0 0.0 0.0 7.77668013996139E-4 4.713139478764479E-5 24-25 0.0 0.0 0.0 0.0016260331201737453 4.713139478764479E-5 26-27 0.0 0.0 0.0 0.0033698947273166024 4.713139478764479E-5 28-29 0.0 0.0 0.0 0.011994939973455597 4.713139478764479E-5 30-31 0.0 0.0 0.0 0.036291173986486486 4.713139478764479E-5 32-33 0.0 0.0 0.0 0.08735804023889962 4.713139478764479E-5 34-35 0.0 0.0 0.0 0.1667980061534749 4.713139478764479E-5 36-37 0.0 0.0 0.0 0.2952781883445946 4.713139478764479E-5 38-39 0.0 0.0 0.0 0.4801510843991313 4.713139478764479E-5 40-41 0.0 0.0 0.0 0.6841357610400579 4.713139478764479E-5 42-43 0.0 0.0 0.0 0.8917259893822393 4.713139478764479E-5 44-45 0.0 0.0 0.0 1.1452457619449807 9.426278957528958E-5 46-47 0.0 0.0 0.0 1.4386386944980696 9.426278957528958E-5 48-49 0.0 0.0 0.0 1.748951797779923 9.426278957528958E-5 50-51 2.3565697393822394E-5 0.0 0.0 2.079578532215251 9.426278957528958E-5 52-53 4.713139478764479E-5 0.0 0.0 2.4080607881877416 1.1782848696911196E-4 54-55 4.713139478764479E-5 0.0 0.0 2.772339338501448 1.4139418436293435E-4 56-57 4.713139478764479E-5 0.0 0.0 3.190701164333977 1.6495988175675675E-4 58-59 4.713139478764479E-5 0.0 0.0 3.6465088833252897 1.8852557915057915E-4 60-61 4.713139478764479E-5 0.0 0.0 4.134719436233108 1.8852557915057915E-4 62-63 4.713139478764479E-5 0.0 0.0 4.62726607746139 1.8852557915057915E-4 64-65 4.713139478764479E-5 0.0 0.0 5.149694022985038 1.8852557915057915E-4 66-67 4.713139478764479E-5 0.0 0.0 5.720195991192085 1.8852557915057915E-4 68-69 4.713139478764479E-5 0.0 0.0 6.356823306286197 1.8852557915057915E-4 70-71 4.713139478764479E-5 0.0 0.0 7.046402743424228 1.8852557915057915E-4 72-73 4.713139478764479E-5 0.0 0.0 7.746704572876448 2.3565697393822393E-4 74-75 4.713139478764479E-5 0.0 0.0 8.456102761522683 2.3565697393822393E-4 76-77 4.713139478764479E-5 0.0 0.0 9.212585213561777 2.3565697393822393E-4 78-79 4.713139478764479E-5 0.0 0.0 10.036984005489865 2.3565697393822393E-4 80-81 4.713139478764479E-5 0.0 0.0 10.916997843267374 2.3565697393822393E-4 82-83 4.713139478764479E-5 0.0 0.0 11.817254615106178 2.3565697393822393E-4 84-85 4.713139478764479E-5 0.0 0.0 12.728587264720076 2.3565697393822393E-4 86-87 4.713139478764479E-5 0.0 0.0 13.68167833011583 2.3565697393822393E-4 88-89 4.713139478764479E-5 0.0 0.0 14.67617432583253 2.3565697393822393E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4260 0.0 53.592022 1 GTATCAA 7390 0.0 40.869358 1 ATCAACG 9505 0.0 31.55291 3 TCAACGC 9555 0.0 31.3878 4 TATCAAC 9645 0.0 31.144112 2 CAACGCA 9890 0.0 30.324614 5 AACGCAG 10175 0.0 29.473143 6 ACGCAGA 11470 0.0 25.730621 7 CGCAGAG 11695 0.0 25.073303 8 CGTATAC 120 1.7227152E-4 23.72709 3 GCAGAGT 12600 0.0 22.971144 9 GTACATG 7260 0.0 22.340158 1 TACATGG 7430 0.0 21.395895 2 GAGTACT 7360 0.0 21.339476 12-13 ACATGGG 7505 0.0 20.54978 3 CAGAGTA 12045 0.0 20.307451 10-11 GTGGTAT 2015 0.0 20.299807 1 GTACTTT 7845 0.0 19.838757 14-15 AGAGTAC 11505 0.0 19.590275 10-11 AGTACTT 7605 0.0 19.404154 12-13 >>END_MODULE