FastQCFastQC Report
Thu 26 May 2016
SRR938797_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938797_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104128
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98910.4700759649603066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80770.38386447972746907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50340.2392440003649968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50550.053.0757331
GTATCAA87050.040.9496151
ATCAACG111650.031.8270653
TCAACGC113200.031.3075564
TATCAAC115650.030.685292
CAACGCA116000.030.5110135
AACGCAG119600.029.6464586
GTGGTAT23550.026.7708071
GTACATG86000.026.4571781
ACGCAGA134150.026.1852077
TACATGG87500.025.7746582
CGCAGAG135450.025.7591388
ACATGGG90450.024.0435243
GCAGAGT142300.023.9214489
TGGTATC25850.023.4608352
GAGTACT85250.021.79786112-13
CATGGGA46000.021.6299484
CAGAGTA139050.020.61610610-11
CGTATAG1404.9213297E-420.3153341
AGAGTAC130400.020.02310410-11
Produced by FastQC (version 0.11.5)