##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938797_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2104128 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63486441889467 33.0 31.0 34.0 30.0 34.0 2 31.69446630623232 33.0 31.0 34.0 30.0 34.0 3 31.697251307905223 33.0 31.0 34.0 28.0 34.0 4 35.26801363795359 37.0 35.0 37.0 33.0 37.0 5 35.304248600845575 37.0 35.0 37.0 33.0 37.0 6 35.3914272325638 37.0 35.0 37.0 33.0 37.0 7 35.31112603415762 37.0 35.0 37.0 33.0 37.0 8 35.29014156933418 37.0 35.0 37.0 32.0 37.0 9 36.861795955379144 39.0 37.0 39.0 33.0 39.0 10-11 36.76615158393406 39.0 37.0 39.0 32.0 39.0 12-13 36.65152785381878 39.0 37.0 39.0 32.0 39.0 14-15 37.8124289491894 40.0 38.0 41.0 32.0 41.0 16-17 37.74636167571555 40.0 37.0 41.0 32.0 41.0 18-19 37.707417752151954 40.0 37.0 41.0 32.0 41.0 20-21 37.61051133771329 40.0 37.0 41.0 32.0 41.0 22-23 37.506093260486054 40.0 37.0 41.0 32.0 41.0 24-25 37.49963785473127 40.0 37.0 41.0 32.0 41.0 26-27 37.406193919761535 40.0 37.0 41.0 32.0 41.0 28-29 37.286847568208785 40.0 37.0 41.0 31.0 41.0 30-31 37.06485679578429 39.5 36.5 41.0 31.0 41.0 32-33 36.83865762919366 39.0 36.0 41.0 30.5 41.0 34-35 36.50274745642851 39.0 35.0 41.0 30.0 41.0 36-37 36.251518681373 39.0 35.0 41.0 30.0 41.0 38-39 36.074940307813975 39.0 35.0 41.0 29.5 41.0 40-41 35.87364647017672 39.0 35.0 41.0 28.5 41.0 42-43 35.59316210800864 39.0 35.0 40.0 27.0 41.0 44-45 35.50553222047328 39.0 35.0 41.0 26.5 41.0 46-47 35.2700104271223 39.0 35.0 41.0 25.5 41.0 48-49 35.00364664126897 39.0 35.0 41.0 24.0 41.0 50-51 33.83227541290264 38.0 33.5 40.0 23.0 40.5 52-53 33.917655437311794 38.0 33.5 39.5 23.0 40.5 54-55 34.43877368677191 38.0 34.0 40.0 23.0 41.0 56-57 34.35345901009825 38.0 34.0 40.0 23.0 41.0 58-59 34.13240639352739 38.0 34.0 40.0 22.0 41.0 60-61 33.85415288423518 38.0 34.0 40.0 21.5 41.0 62-63 33.49714893770721 37.0 33.0 40.0 20.0 41.0 64-65 33.111206637619006 36.5 33.0 40.0 18.5 41.0 66-67 32.7219287514828 36.0 32.5 39.0 17.0 41.0 68-69 32.32508383520394 35.5 32.0 39.0 13.0 41.0 70-71 31.93517385824436 35.0 31.5 38.5 10.0 40.0 72-73 31.51987901876692 35.0 31.0 37.5 7.5 40.0 74-75 31.070344817425557 35.0 31.0 37.0 6.0 39.0 76-77 30.631700637984 35.0 31.0 36.5 2.0 39.0 78-79 30.210030711059403 35.0 30.0 36.0 2.0 38.5 80-81 29.784423286035832 34.0 30.0 35.5 2.0 37.5 82-83 29.330303099431212 34.0 29.0 35.0 2.0 37.0 84-85 28.923189796438237 34.0 29.0 35.0 2.0 36.5 86-87 28.536005889375552 34.0 29.0 35.0 2.0 36.0 88-89 28.167961027085802 34.0 29.0 35.0 2.0 36.0 90-91 27.755655311844144 34.0 27.0 35.0 2.0 35.0 92-93 27.30681213310217 34.0 26.5 35.0 2.0 35.0 94-95 26.81019667054476 34.0 25.5 35.0 2.0 35.0 96-97 26.233622194087054 33.0 24.5 35.0 2.0 35.0 98-99 25.547271601347447 33.0 21.0 35.0 2.0 35.0 100-101 23.40850414043252 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32939052434027616 1101 2 0.06413936157644784 1101 3 0.2966214543911718 1101 4 0.2798348284468304 1101 5 0.22159289550818784 1101 6 0.23876605038187648 1101 7 0.28370990756065595 1101 8 0.28440158947947225 1101 9 0.36436629946975785 1101 10-11 0.41787303435684464 1101 12-13 0.3600947188264527 1101 14-15 0.4589626652951253 1101 16-17 0.5222397206307576 1101 18-19 0.539375818300492 1101 20-21 0.50946217984378 1101 22-23 0.46368794702149785 1101 24-25 0.46240713192934635 1101 26-27 0.4642496615973144 1101 28-29 0.45272204140691485 1101 30-31 0.3717873143527797 1101 32-33 0.2748601226190317 1101 34-35 0.12411149107493458 1101 36-37 0.22670794762088065 1101 38-39 0.20638780143237767 1101 40-41 0.3207988670627486 1101 42-43 0.29769110703975343 1101 44-45 0.32923351024559366 1101 46-47 0.22646310446811668 1101 48-49 0.30633657133002856 1101 50-51 0.3540436567283578 1101 52-53 0.33834380075837345 1101 54-55 0.2114203526316416 1101 56-57 0.17924595164377166 1101 58-59 0.2669643835966937 1101 60-61 0.14051367987204344 1101 62-63 0.06275465326795882 1101 64-65 0.15849450660815734 1101 66-67 0.04239123794581445 1101 68-69 0.016291208973910898 1101 70-71 -0.05691327672927571 1101 72-73 -0.2264848735831997 1101 74-75 -0.1200293776847623 1101 76-77 -0.11979350941821565 1101 78-79 -0.28309762729401555 1101 80-81 -0.36263793856192805 1101 82-83 -0.4029741903171953 1101 84-85 -0.40324314034894115 1101 86-87 -0.41139550580528805 1101 88-89 -0.5413957228059054 1101 90-91 -0.6129193039554011 1101 92-93 -0.8358470026720681 1101 94-95 -0.8215065047147263 1101 96-97 -1.0900289124327287 1101 98-99 -1.1035216517851616 1101 100-101 -0.7059040402531913 1102 1 0.26683366137268294 1102 2 0.2995712972374598 1102 3 0.34007185367305226 1102 4 0.2992913349887303 1102 5 0.24980351290071212 1102 6 0.2875167367900815 1102 7 0.2516903578444385 1102 8 0.31855533681235926 1102 9 0.36210039252949144 1102 10-11 0.25603824242718076 1102 12-13 0.3281759774002282 1102 14-15 0.20462613581553768 1102 16-17 0.4086756359396375 1102 18-19 0.34559923581297625 1102 20-21 0.33340632698538286 1102 22-23 0.3675500189915226 1102 24-25 0.361969907632421 1102 26-27 0.352041929652124 1102 28-29 0.4145795278661808 1102 30-31 0.2595950329244019 1102 32-33 0.1603842041491248 1102 34-35 0.2909026061665969 1102 36-37 0.41762137204303684 1102 38-39 0.3308270236584292 1102 40-41 0.3926518754050079 1102 42-43 0.35632961763019466 1102 44-45 0.429070697258652 1102 46-47 0.545484868927268 1102 48-49 0.4862408693091993 1102 50-51 0.5085847915764177 1102 52-53 0.3730469279429727 1102 54-55 0.6120129939304135 1102 56-57 0.4015717964577661 1102 58-59 0.32512215515598797 1102 60-61 0.35269917320451327 1102 62-63 0.40240133794166155 1102 64-65 0.3819591304969876 1102 66-67 0.19353173937333423 1102 68-69 0.29923778969855874 1102 70-71 0.301574933259964 1102 72-73 0.29342020452043016 1102 74-75 0.3035179191333377 1102 76-77 0.24045377990258743 1102 78-79 7.681325370718639E-4 1102 80-81 -0.23288500499003462 1102 82-83 -0.07629099581899368 1102 84-85 -0.15134905896068673 1102 86-87 -0.18187655891251353 1102 88-89 -0.13600683583073803 1102 90-91 0.06527201295546803 1102 92-93 0.01665349883651146 1102 94-95 -0.03987524421597399 1102 96-97 0.009254335559713667 1102 98-99 -0.11786834314563421 1102 100-101 0.225194759976187 1103 1 0.046019168064457716 1103 2 0.13731260900672382 1103 3 0.08798728252069665 1103 4 0.23723355107920696 1103 5 0.18367275893045587 1103 6 0.27153200350441864 1103 7 0.1855822622562755 1103 8 0.18127625603774788 1103 9 0.2151124467887655 1103 10-11 0.18712856377339193 1103 12-13 0.2854850679431209 1103 14-15 0.3713895621657244 1103 16-17 0.3251127815877197 1103 18-19 0.350924207364109 1103 20-21 0.3846188021870418 1103 22-23 0.4595367562852104 1103 24-25 0.36518009685508446 1103 26-27 0.31677340264837284 1103 28-29 0.32587699717665686 1103 30-31 0.26468989601742265 1103 32-33 0.18667120025188666 1103 34-35 0.29870615295867253 1103 36-37 0.34264201634671565 1103 38-39 0.2203344304317767 1103 40-41 0.23073809810165358 1103 42-43 0.18966513288179954 1103 44-45 0.26090018538062765 1103 46-47 0.24656945015001241 1103 48-49 0.32177676522163523 1103 50-51 0.32027953785289043 1103 52-53 0.2368421062392585 1103 54-55 0.3090193181366132 1103 56-57 0.42933385323463824 1103 58-59 0.37034224357402934 1103 60-61 0.3798008640233377 1103 62-63 0.46072275464440793 1103 64-65 0.2551562785619623 1103 66-67 0.2772912181298395 1103 68-69 0.14393321069129428 1103 70-71 0.25918135212785387 1103 72-73 0.2472104483246227 1103 74-75 0.30171280136010736 1103 76-77 0.3022512441778744 1103 78-79 0.2206196446064368 1103 80-81 0.09556536308279462 1103 82-83 0.3507308871429089 1103 84-85 0.3744890494168729 1103 86-87 0.2557300613632165 1103 88-89 -0.1066700214985552 1103 90-91 -0.20331465787362646 1103 92-93 -0.16521350149581338 1103 94-95 -0.12238221796246407 1103 96-97 -0.20219998010679419 1103 98-99 -0.1039022199444588 1103 100-101 0.024405241240220477 1104 1 0.21532548115922978 1104 2 0.292959762350403 1104 3 0.26755193797273336 1104 4 0.3193931075507379 1104 5 0.14046915401412008 1104 6 0.2743235181780861 1104 7 0.16264633136048445 1104 8 0.2676894541035466 1104 9 0.30625036078775736 1104 10-11 0.3185460783572296 1104 12-13 0.26505022241459386 1104 14-15 0.423306545293876 1104 16-17 0.3820471555428995 1104 18-19 0.26030128141360365 1104 20-21 0.3702789799377584 1104 22-23 0.4116608324754907 1104 24-25 0.25786621862580006 1104 26-27 0.348096103541117 1104 28-29 0.493305500168276 1104 30-31 0.38848463934677824 1104 32-33 0.33966257220599516 1104 34-35 0.4350996397571194 1104 36-37 0.6349137024834519 1104 38-39 0.5987718009986125 1104 40-41 0.34827513781861796 1104 42-43 0.473254629520909 1104 44-45 0.6994172493499278 1104 46-47 0.7488240199204128 1104 48-49 0.9007645542162521 1104 50-51 0.9145839796248083 1104 52-53 0.8882618165085816 1104 54-55 0.9776397290292138 1104 56-57 0.7862592448401315 1104 58-59 0.80205168452256 1104 60-61 0.8240015097544742 1104 62-63 0.7610513589369816 1104 64-65 0.6669705634920717 1104 66-67 0.6356647641119864 1104 68-69 0.705974798970324 1104 70-71 0.5146283986813671 1104 72-73 0.47508467546828115 1104 74-75 0.47495612822281785 1104 76-77 0.4147681484609578 1104 78-79 0.6045350009312571 1104 80-81 0.595640964525959 1104 82-83 0.4905805824915461 1104 84-85 0.4895379782716738 1104 86-87 0.5405606193168353 1104 88-89 0.4595041961637385 1104 90-91 0.41331691109335367 1104 92-93 0.40654723864980014 1104 94-95 0.4571786948438721 1104 96-97 0.5692753209266748 1104 98-99 0.5798090374347886 1104 100-101 0.7416860344815 1105 1 0.12135508142300111 1105 2 0.08557257287320752 1105 3 0.03980432450862992 1105 4 0.182215336232872 1105 5 0.13012505972717037 1105 6 0.19570147561941553 1105 7 0.2109408824693233 1105 8 0.20390363655526755 1105 9 0.1700768562624546 1105 10-11 0.3059081197063023 1105 12-13 0.19738000431457436 1105 14-15 0.4178408757079026 1105 16-17 0.19351545309852014 1105 18-19 0.25130750894076215 1105 20-21 0.18781750690907728 1105 22-23 0.32456178318789597 1105 24-25 0.29545623195099324 1105 26-27 0.2591616701153754 1105 28-29 0.24641658114759224 1105 30-31 0.30514585952850126 1105 32-33 0.3816149761056167 1105 34-35 0.3058696719287681 1105 36-37 0.16462175788347366 1105 38-39 0.19970475948371558 1105 40-41 0.20849475960169173 1105 42-43 0.28981791052245853 1105 44-45 0.2606336266537923 1105 46-47 0.062484487023965585 1105 48-49 0.013528253507850252 1105 50-51 0.04583906436561591 1105 52-53 0.18934553694879952 1105 54-55 0.20625796717749978 1105 56-57 0.05523422937861966 1105 58-59 0.0921065168065951 1105 60-61 0.39687938037720727 1105 62-63 0.2653364419414075 1105 64-65 0.3072625861312446 1105 66-67 0.18106820720093708 1105 68-69 0.18932001702157208 1105 70-71 0.16409368392435653 1105 72-73 0.04385378287556918 1105 74-75 0.066508044767712 1105 76-77 2.705647492931007E-4 1105 78-79 0.00836633483557847 1105 80-81 0.0730539849741767 1105 82-83 0.05788206543120822 1105 84-85 -0.020104251073792767 1105 86-87 0.03738613149777592 1105 88-89 -0.05989439483873227 1105 90-91 0.06654075869843012 1105 92-93 0.08522039687620264 1105 94-95 0.22678774777980593 1105 96-97 0.35632076704744264 1105 98-99 0.24405835898160788 1105 100-101 0.44440282246658924 1106 1 0.07650656121326094 1106 2 0.043477317652190095 1106 3 0.1607145997299142 1106 4 0.14258161564742267 1106 5 0.08579926292070894 1106 6 0.03848703181050439 1106 7 0.10354011383158479 1106 8 0.21442936334644003 1106 9 0.1705512767711994 1106 10-11 0.22313943605104214 1106 12-13 0.10571450138100857 1106 14-15 0.25064734083512263 1106 16-17 0.1507905863858099 1106 18-19 0.26805880929294545 1106 20-21 0.23644560905487566 1106 22-23 0.2501279791962645 1106 24-25 0.23047688263402932 1106 26-27 0.03775220121765699 1106 28-29 0.11330121661856651 1106 30-31 0.17479715088963843 1106 32-33 0.2188031354879456 1106 34-35 0.22925984645047492 1106 36-37 0.29908171239267034 1106 38-39 0.10841994074869632 1106 40-41 0.11523286991594262 1106 42-43 0.18791147657691454 1106 44-45 0.015263530832321237 1106 46-47 -0.0445169458024921 1106 48-49 0.09476463543931857 1106 50-51 0.10791880527079911 1106 52-53 -0.08258756402050693 1106 54-55 -0.20480540430715166 1106 56-57 -0.15411601538793462 1106 58-59 -0.12005007830801873 1106 60-61 0.05506227330929647 1106 62-63 -0.04562348482949119 1106 64-65 -0.04261201764155231 1106 66-67 -0.06446234031476905 1106 68-69 0.13169920717810868 1106 70-71 0.03170047158100431 1106 72-73 -0.03897755106864764 1106 74-75 -0.13673307945585478 1106 76-77 -0.3228100408255301 1106 78-79 -0.24414111146212392 1106 80-81 -0.22385721230632427 1106 82-83 -0.28595228529180616 1106 84-85 -0.19104676010786648 1106 86-87 -0.10694602598687553 1106 88-89 -0.01705642283337383 1106 90-91 0.04703714985048535 1106 92-93 0.06599536660320382 1106 94-95 0.16094875041398993 1106 96-97 0.37732128737961546 1106 98-99 0.3655810288212784 1106 100-101 0.630268295557574 1107 1 0.15769683350007213 1107 2 0.1364940584224783 1107 3 0.07374377583061786 1107 4 0.04042420313466266 1107 5 0.08268831865679971 1107 6 -0.01425355077299173 1107 7 -0.14848203513429326 1107 8 -0.005408977358150935 1107 9 0.056234926657985795 1107 10-11 -4.28353911061663E-5 1107 12-13 0.10740793529191706 1107 14-15 0.12572025175826695 1107 16-17 0.08172112450713342 1107 18-19 0.22060816934268246 1107 20-21 0.1546823730534257 1107 22-23 0.013997009357794354 1107 24-25 0.07404429737575668 1107 26-27 0.08750900838791864 1107 28-29 -0.10807711071218762 1107 30-31 0.028433311891980395 1107 32-33 0.15624134010779045 1107 34-35 0.1912804703286568 1107 36-37 0.26774221614086713 1107 38-39 0.1028784759606225 1107 40-41 0.29839203041382234 1107 42-43 0.4901307137984361 1107 44-45 0.4830936829891499 1107 46-47 0.6110666348687843 1107 48-49 0.566795231044722 1107 50-51 0.6729598066668387 1107 52-53 0.5633087769421721 1107 54-55 0.5668833370262476 1107 56-57 0.4878390297194741 1107 58-59 0.4979695366659982 1107 60-61 0.3937307544392681 1107 62-63 0.2984487339384714 1107 64-65 0.4486399620395858 1107 66-67 0.5184451807138473 1107 68-69 0.44891111224726643 1107 70-71 0.44661066737880617 1107 72-73 0.24238693315842852 1107 74-75 0.24486591633256083 1107 76-77 0.09774861052769523 1107 78-79 -0.012874936734000642 1107 80-81 0.1044095841883248 1107 82-83 0.21414191820181827 1107 84-85 -0.015807614626233146 1107 86-87 0.0510040215254719 1107 88-89 0.07844773596345433 1107 90-91 0.04671864115162094 1107 92-93 0.1670399343581579 1107 94-95 0.1968252652366651 1107 96-97 0.31674292720082775 1107 98-99 0.5545321204321247 1107 100-101 0.4433971889304793 1108 1 0.1765965834432386 1108 2 0.1678852837009508 1108 3 0.0597565059965639 1108 4 0.15333054548519698 1108 5 0.15817024636764643 1108 6 0.21459164131611885 1108 7 0.30254206125125194 1108 8 0.20070832202807765 1108 9 0.2258382455941117 1108 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2316 90-91 -2.2721838307628985 2316 92-93 -2.5465016066532655 2316 94-95 -2.5924412023430534 2316 96-97 -2.8898384164889492 2316 98-99 -3.2165918381617153 2316 100-101 -3.414352343999777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7632.0 3 1963.0 4 1876.0 5 2164.0 6 2402.0 7 3094.0 8 3788.0 9 4632.0 10 5804.0 11 7839.0 12 10472.0 13 13493.0 14 14336.0 15 14408.0 16 17870.0 17 23403.0 18 19619.0 19 13665.0 20 13544.0 21 14406.0 22 15838.0 23 18056.0 24 20438.0 25 23466.0 26 27233.0 27 32127.0 28 37693.0 29 44777.0 30 53197.0 31 64886.0 32 79247.0 33 98976.0 34 130013.0 35 181299.0 36 263988.0 37 392006.0 38 362793.0 39 61679.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08017990399281 19.46597190618639 14.570119333014706 22.883728856806094 2 15.819185657091534 21.31151908176158 39.329969097745504 23.539326163401377 3 18.25822520356683 25.561657988695963 31.283077529018797 24.897039278718406 4 13.087818589064115 17.586887061568355 37.40492044431673 31.920373905050802 5 13.611619735735141 37.10148044660837 34.37156202534537 14.915337792311117 6 30.289559190325903 36.362590371355786 18.380937075957107 14.966913362361204 7 26.772855325876353 33.66610006299999 22.02422527844806 17.5368193326756 8 25.554329339122173 34.07483238054573 21.24831263161949 19.122525648712607 9 26.476741587458573 16.593093085953658 21.377578918839244 35.55258640774853 10-11 24.227240016746237 26.660055977685005 28.160370147865628 20.952333857703138 12-13 25.260233587922375 24.828601147136617 28.295426312817618 21.615738952123394 14-15 22.6995874911708 25.990742741748935 26.956433257184077 24.353236509896195 16-17 21.49001569330979 28.883226417063558 27.65888198302954 21.967875906597108 18-19 21.332807111601408 27.310693911321337 30.315459625756493 21.041039351320766 20-21 22.407391336909967 26.558812935315103 30.077282361766095 20.95651336600884 22-23 22.141819211643092 26.513518905447437 29.970507535285503 21.374154347623968 24-25 21.876127443274367 26.652313082539607 30.124309205026595 21.34725026915943 26-27 21.607834640716092 26.85651879522754 30.026082648724252 21.509563915332112 28-29 21.525943551584025 26.95317098157521 30.073773842496426 21.447111624344345 30-31 21.746834413673707 26.719491233038468 29.969880771415575 21.563793581872254 32-33 21.557607882902428 26.913302576160852 29.784252026537867 21.744837514398853 34-35 22.00644950627671 26.924924982184013 29.567434998462698 21.501190513076576 36-37 22.02996164365246 26.7698654961196 29.45110908698045 21.749063773247492 38-39 21.70884187459868 26.858187703346886 29.664046480651763 21.76892394140268 40-41 21.903680945578362 26.64837354621956 29.648935322252772 21.7990101859493 42-43 21.825299538103422 26.809289876685146 29.3051642228648 22.060246362346632 44-45 21.739990937422856 26.899763026499684 29.269259289499683 22.09098674657778 46-47 21.972967307803412 27.067022607936675 28.83876309294565 22.121246991314266 48-49 22.042520108442083 27.102426384453643 28.590305445838414 22.26474806126586 50-51 21.864839686396824 27.363328636320876 28.43982945595035 22.332002221331948 52-53 21.948210464588218 27.33906979209595 28.375796467297743 22.33692327601809 54-55 21.782072790744813 27.259225021111238 28.353714802363694 22.60498738578025 56-57 21.779571486335463 27.537452338703478 28.258834436339974 22.42414173862108 58-59 21.808878126520618 27.07842668855202 28.380188534482205 22.732506650445156 60-61 21.792080474018768 27.04098040731336 28.71088214327635 22.456056975391526 62-63 21.643179319835717 26.952342189974782 28.797138311369768 22.607340178819733 64-65 21.576835520994695 26.999634401417694 28.63081062351632 22.79271945407129 66-67 21.547322166509893 27.091833164859057 28.42460674809311 22.936237920537934 68-69 21.477482512138213 26.92495444438872 28.58554304915707 23.012019994315995 70-71 21.527805474045607 26.951126111011735 28.487252008281256 23.0338164066614 72-73 21.627883929989288 26.890053956340616 28.313336015827513 23.168726097842583 74-75 21.48908730608815 27.080381888770113 28.204830033804363 23.225700771337376 76-77 21.64543266418731 26.900997488016632 28.285071198327316 23.16849864946874 78-79 21.51424461406969 26.950667090875633 28.361273285647215 23.173815009407463 80-81 21.534700553015785 26.88421946580236 28.284648578745376 23.296431402436475 82-83 21.642466755708014 26.77375500260798 28.274614979715047 23.30916326196896 84-85 21.556067886074835 26.578989080325872 28.2809868192391 23.583956214360192 86-87 21.61567960480123 26.4448177789789 28.44344407553984 23.49605854068003 88-89 21.74849106433597 26.29875672631098 28.34681202048509 23.60594018886796 90-91 21.729695655703647 26.347701300375835 28.369826314105474 23.552776729815044 92-93 21.84162085481653 26.162814714456644 28.328593160923344 23.666971269803483 94-95 21.764347835846255 25.994870284975942 28.347967659059492 23.89281422011831 96-97 21.96925303207227 25.71321455776757 28.357749902691914 23.959782507468248 98-99 21.898654835940608 25.6381321238358 28.333832780359913 24.12938025986368 100-101 22.281643843988956 25.277162074277236 28.592912443619756 23.848281638114045 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2223.0 1 1392.0 2 683.0 3 807.5 4 737.0 5 774.0 6 1200.5 7 2054.0 8 3148.5 9 3698.5 10 3435.0 11 3250.0 12 3456.5 13 3690.5 14 3967.5 15 4221.5 16 4393.5 17 4705.0 18 5069.0 19 5338.0 20 5664.5 21 6221.5 22 7023.5 23 7863.5 24 8930.0 25 10620.0 26 12953.0 27 15769.0 28 18364.5 29 21254.0 30 25319.5 31 29821.0 32 34164.5 33 37871.5 34 41613.5 35 45362.0 36 49815.5 37 54190.5 38 57024.5 39 58451.5 40 59531.5 41 62665.5 42 65427.5 43 68599.0 44 72565.0 45 75395.5 46 77484.0 47 78818.5 48 79683.0 49 79356.5 50 77520.5 51 74956.0 52 72876.5 53 71227.5 54 68259.5 55 63451.0 56 57349.0 57 50750.5 58 45961.0 59 40838.0 60 34226.5 61 27915.5 62 22526.0 63 17592.0 64 13029.0 65 9823.5 66 7496.5 67 5891.0 68 4394.5 69 3167.0 70 2424.5 71 1794.0 72 1460.5 73 1167.5 74 842.5 75 658.0 76 497.0 77 331.0 78 187.0 79 115.5 80 99.0 81 80.0 82 53.5 83 42.5 84 34.5 85 21.5 86 16.5 87 16.5 88 16.0 89 10.0 90 9.5 91 9.5 92 7.5 93 6.5 94 4.0 95 4.0 96 3.5 97 3.5 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16405846032180552 2 0.09581166164796058 3 0.08887292027861422 4 0.10474647930163944 5 0.12356662712534598 6 0.04096708945463394 7 0.04529192140402105 8 0.0475731514432582 9 0.048381087082154696 10-11 0.04602856860419138 12-13 0.05063855430848314 14-15 0.048547426772515744 16-17 0.047644439881984364 18-19 0.05158906682483195 20-21 0.053490091857529586 22-23 0.06594180582169906 24-25 0.08314608236761262 26-27 0.08423917176141375 28-29 0.07383105970739423 30-31 0.06266253764029564 32-33 0.05206432308300636 34-35 0.2990787632691547 36-37 0.47290373969644434 38-39 0.4511607658849652 40-41 0.42711279922134016 42-43 0.4567925525443319 44-45 0.4657986586367369 46-47 0.4645629923654835 48-49 0.48034150013687377 50-51 0.46998091370867173 52-53 0.4770147063296529 54-55 0.4688165358761444 56-57 0.4466933570581258 58-59 0.4261860495179 60-61 0.39664887307236063 62-63 0.4543687456276424 64-65 0.4895852343583661 66-67 0.4912486312619765 68-69 0.5005874167351035 70-71 0.4982824238829577 72-73 0.4897753368616358 74-75 0.483216800498829 76-77 0.46418278735894397 78-79 0.43954075037260093 80-81 0.4439606335736229 82-83 0.45603214253125285 84-85 0.4543449828147337 86-87 0.46525211393983634 88-89 0.47618300787784773 90-91 0.4808642820208656 92-93 0.4822187623566627 94-95 0.4871139018158591 96-97 0.4877554977643946 98-99 0.4821712367308452 100-101 0.4688878243148706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2104128.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.42510544998215 #Duplication Level Percentage of deduplicated Percentage of total 1 79.77504705036789 46.608655361950305 2 11.672919029095011 13.639830503679583 3 3.4454344401064505 6.038996114526587 4 1.527512327897995 3.569802753343523 5 0.8396351048128784 2.4527884769099613 6 0.5158546160938052 1.8083316205284374 7 0.3699621512947002 1.5130554391326578 8 0.2812419510972076 1.3145272519850462 9 0.20548357954442978 1.0804859822810793 >10 1.2410130962978914 13.49819663639225 >50 0.09166148850538122 3.64898225481314 >100 0.033092073460898444 3.211385164603555 >500 5.715457127244352E-4 0.23952048238041979 >1k 3.265975501282393E-4 0.2781605123999601 >5k 2.449481625961795E-4 1.0972814450735522 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9891 0.4700759649603066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8077 0.38386447972746907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5034 0.2392440003649968 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.7525625817440767E-5 0.0 12-13 4.7525625817440767E-5 0.0 0.0 1.4257687745232228E-4 0.0 14-15 4.7525625817440767E-5 0.0 0.0 1.6633969036104269E-4 0.0 16-17 4.7525625817440767E-5 0.0 0.0 2.3762812908720383E-4 4.7525625817440767E-5 18-19 4.7525625817440767E-5 0.0 0.0 2.8515375490464456E-4 9.505125163488153E-5 20-21 4.7525625817440767E-5 0.0 0.0 5.227818839918484E-4 9.505125163488153E-5 22-23 4.7525625817440767E-5 0.0 0.0 0.0014495315874319434 1.188140645436019E-4 24-25 4.7525625817440767E-5 0.0 0.0 0.0027564862974115644 1.4257687745232228E-4 26-27 4.7525625817440767E-5 0.0 0.0 0.005441684156096967 1.4257687745232228E-4 28-29 4.7525625817440767E-5 0.0 0.0 0.014519078687228154 1.4257687745232228E-4 30-31 4.7525625817440767E-5 0.0 0.0 0.040420544757733365 1.4257687745232228E-4 32-33 4.7525625817440767E-5 0.0 0.0 0.09203337439547404 1.4257687745232228E-4 34-35 4.7525625817440767E-5 0.0 0.0 0.17776960337013717 1.4257687745232228E-4 36-37 4.7525625817440767E-5 0.0 0.0 0.30722940809684585 1.4257687745232228E-4 38-39 4.7525625817440767E-5 0.0 0.0 0.49112981719743287 1.4257687745232228E-4 40-41 4.7525625817440767E-5 0.0 0.0 0.6893116768561609 1.4257687745232228E-4 42-43 4.7525625817440767E-5 0.0 0.0 0.8906539906317487 1.4257687745232228E-4 44-45 4.7525625817440767E-5 0.0 0.0 1.1379298217598928 1.4257687745232228E-4 46-47 4.7525625817440767E-5 0.0 0.0 1.4310203561760502 1.6633969036104269E-4 48-49 4.7525625817440767E-5 0.0 0.0 1.738083424582535 2.3762812908720383E-4 50-51 4.7525625817440767E-5 0.0 0.0 2.0591903154180735 2.3762812908720383E-4 52-53 4.7525625817440767E-5 0.0 0.0 2.3728594458131824 2.851537549046446E-4 54-55 4.7525625817440767E-5 0.0 0.0 2.729919472579615 3.3267938072208537E-4 56-57 4.7525625817440767E-5 0.0 0.0 3.1381408355385223 3.3267938072208537E-4 58-59 4.7525625817440767E-5 0.0 0.0 3.5806994631505304 3.3267938072208537E-4 60-61 4.7525625817440767E-5 0.0 0.0 4.06206276424248 3.3267938072208537E-4 62-63 4.7525625817440767E-5 0.0 0.0 4.543235962831158 3.3267938072208537E-4 64-65 4.7525625817440767E-5 0.0 0.0 5.04681749399276 3.3267938072208537E-4 66-67 4.7525625817440767E-5 2.3762812908720383E-5 0.0 5.612229864342854 3.3267938072208537E-4 68-69 4.7525625817440767E-5 4.7525625817440767E-5 0.0 6.243655328953372 3.3267938072208537E-4 70-71 4.7525625817440767E-5 4.7525625817440767E-5 0.0 6.922630182194239 3.8020500653952613E-4 72-73 4.7525625817440767E-5 4.7525625817440767E-5 0.0 7.599157465705508 3.8020500653952613E-4 74-75 4.7525625817440767E-5 4.7525625817440767E-5 0.0 8.305863521610853 3.8020500653952613E-4 76-77 4.7525625817440767E-5 4.7525625817440767E-5 0.0 9.061829888675973 3.8020500653952613E-4 78-79 4.7525625817440767E-5 7.128843872616115E-5 0.0 9.885330170027679 3.8020500653952613E-4 80-81 9.505125163488153E-5 9.505125163488153E-5 0.0 10.770067220245156 3.8020500653952613E-4 82-83 9.505125163488153E-5 9.505125163488153E-5 0.0 11.675810597073943 4.277306323569669E-4 84-85 9.505125163488153E-5 9.505125163488153E-5 0.0 12.584072832071053 4.277306323569669E-4 86-87 9.505125163488153E-5 9.505125163488153E-5 0.0 13.503265961006175 4.277306323569669E-4 88-89 9.505125163488153E-5 9.505125163488153E-5 0.0 14.474879855217933 4.277306323569669E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5055 0.0 53.075733 1 GTATCAA 8705 0.0 40.949615 1 ATCAACG 11165 0.0 31.827065 3 TCAACGC 11320 0.0 31.307556 4 TATCAAC 11565 0.0 30.68529 2 CAACGCA 11600 0.0 30.511013 5 AACGCAG 11960 0.0 29.646458 6 GTGGTAT 2355 0.0 26.770807 1 GTACATG 8600 0.0 26.457178 1 ACGCAGA 13415 0.0 26.185207 7 TACATGG 8750 0.0 25.774658 2 CGCAGAG 13545 0.0 25.759138 8 ACATGGG 9045 0.0 24.043524 3 GCAGAGT 14230 0.0 23.921448 9 TGGTATC 2585 0.0 23.460835 2 GAGTACT 8525 0.0 21.797861 12-13 CATGGGA 4600 0.0 21.629948 4 CAGAGTA 13905 0.0 20.616106 10-11 CGTATAG 140 4.9213297E-4 20.315334 1 AGAGTAC 13040 0.0 20.023104 10-11 >>END_MODULE