FastQCFastQC Report
Thu 26 May 2016
SRR938797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938797_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104128
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94780.45044788149770354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82920.3940824892782188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52480.24941448428992913No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT21970.10441379992091736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54650.056.6635171
GTATCAA92700.039.315341
ATCAACG121400.029.745373
TCAACGC122350.029.514414
TATCAAC123850.029.3485162
CAACGCA123800.029.1687245
AACGCAG127650.028.2889776
GTGGTAT22600.027.193281
ACGCAGA144200.024.6802437
CGCAGAG145050.024.3720468
GTACATG88550.023.6725181
TGGTATC25550.023.2152042
GCAGAGT155500.022.3374779
TACATGG91250.022.3088842
AATACCG1503.058599E-522.144215
GAGTACT91250.022.05096812-13
ACATGGG91800.021.6583213
CAGAGTA150150.020.98612610-11
AGTACTT94200.020.37803512-13
AGAGTAC140050.020.04292910-11
Produced by FastQC (version 0.11.5)