##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938797_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2104128 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.968967667366243 31.0 31.0 34.0 30.0 34.0 2 32.18745675168051 34.0 31.0 34.0 30.0 34.0 3 32.26718479103933 34.0 31.0 34.0 30.0 34.0 4 35.9217908796423 37.0 35.0 37.0 35.0 37.0 5 35.82869910955988 37.0 35.0 37.0 35.0 37.0 6 35.85177422666302 37.0 35.0 37.0 35.0 37.0 7 35.78454162484412 37.0 35.0 37.0 33.0 37.0 8 35.75564699485963 37.0 35.0 37.0 33.0 37.0 9 37.396175993095476 39.0 37.0 39.0 34.0 39.0 10-11 37.328936500060834 39.0 37.0 39.0 34.0 39.0 12-13 37.26197431905283 39.0 37.0 39.0 33.0 39.0 14-15 38.48202699645649 40.0 38.0 41.0 33.5 41.0 16-17 38.41446219051312 40.0 38.0 41.0 33.0 41.0 18-19 38.44532105461265 40.0 38.0 41.0 33.5 41.0 20-21 38.50599987263132 40.0 38.0 41.0 34.0 41.0 22-23 38.44732307160021 40.0 38.0 41.0 34.0 41.0 24-25 38.37160025435715 40.0 38.0 41.0 34.0 41.0 26-27 38.20993708557654 40.0 38.0 41.0 33.0 41.0 28-29 38.03660685091401 40.0 37.0 41.0 33.0 41.0 30-31 37.78651512645619 40.0 37.0 41.0 33.0 41.0 32-33 37.53738698406181 40.0 36.5 41.0 32.0 41.0 34-35 37.472805836907256 40.0 36.0 41.0 32.0 41.0 36-37 37.371882318946376 40.0 36.0 41.0 32.0 41.0 38-39 37.2598140417313 40.0 36.0 41.0 31.0 41.0 40-41 37.08152831006478 40.0 36.0 41.0 31.0 41.0 42-43 36.88188242350275 40.0 36.0 41.0 31.0 41.0 44-45 36.5922729035496 39.5 35.0 41.0 30.0 41.0 46-47 36.238202476275205 39.0 35.0 41.0 30.0 41.0 48-49 35.91703665366366 39.0 35.0 41.0 28.5 41.0 50-51 35.65909274530827 39.0 35.0 41.0 28.0 41.0 52-53 35.44377528363293 39.0 35.0 40.5 27.0 41.0 54-55 35.19035462671472 38.5 35.0 40.0 26.5 41.0 56-57 34.93040893899991 38.0 34.0 40.0 26.0 41.0 58-59 34.621482153177 38.0 34.0 40.0 26.0 41.0 60-61 34.27906809851872 37.5 34.0 40.0 25.5 41.0 62-63 33.9027730252152 37.0 33.0 40.0 24.0 41.0 64-65 33.53458154636981 36.5 33.0 39.5 23.0 41.0 66-67 33.1376496581957 36.0 33.0 39.0 22.0 40.5 68-69 32.691770652735954 35.5 32.0 39.0 21.5 40.0 70-71 32.236030792803476 35.0 32.0 38.0 20.0 40.0 72-73 31.900888871779664 35.0 31.5 37.5 19.5 39.5 74-75 31.48884787427381 35.0 31.0 37.0 18.0 39.0 76-77 29.424942541518387 33.0 28.5 35.0 12.0 37.5 78-79 30.477906287069988 34.0 30.5 36.0 12.0 38.0 80-81 30.529536225933022 34.0 31.0 35.5 11.0 37.0 82-83 30.229389086595493 34.0 31.0 35.0 7.0 37.0 84-85 29.84965648477659 34.0 30.0 35.0 4.0 36.5 86-87 29.319849600404538 34.0 29.5 35.0 2.0 36.0 88-89 28.819707261155216 34.0 29.0 35.0 2.0 35.5 90-91 28.420937557030754 34.0 29.0 35.0 2.0 35.0 92-93 28.139353451881256 34.0 29.0 35.0 2.0 35.0 94-95 27.76412604176172 34.0 27.5 35.0 2.0 35.0 96-97 27.266315309714997 33.0 27.0 35.0 2.0 35.0 98-99 26.566233137907957 33.0 25.5 35.0 2.0 35.0 100-101 23.640089623825165 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07020105734742899 1101 2 0.07319764106178184 1101 3 0.18266034873034442 1101 4 0.21238768649146067 1101 5 0.11067460874200208 1101 6 0.19449852876563511 1101 7 0.124488851447353 1101 8 0.13212743403718719 1101 9 0.17301191353207912 1101 10-11 0.24482387482166956 1101 12-13 0.1822331324445443 1101 14-15 0.1972510948101558 1101 16-17 0.04286110586841119 1101 18-19 0.07169447326841549 1101 20-21 0.12091885311436101 1101 22-23 0.07920836346988125 1101 24-25 0.024013407661733766 1101 26-27 0.13621198834550086 1101 28-29 0.15268753234252586 1101 30-31 -0.10245900373020334 1101 32-33 0.011586132477496847 1101 34-35 0.014238168619421288 1101 36-37 -0.04856149732130888 1101 38-39 0.025594355230410315 1101 40-41 0.1090553183699896 1101 42-43 0.13769153178473204 1101 44-45 0.10806577812508777 1101 46-47 0.03277670983599279 1101 48-49 -0.1764257096790729 1101 50-51 -0.2207565035322716 1101 52-53 -0.19373665073035085 1101 54-55 -0.1240489597525638 1101 56-57 -0.2770484647451923 1101 58-59 -0.08668782185753798 1101 60-61 -0.14222691240927077 1101 62-63 -0.20936619605000573 1101 64-65 -0.2033277590676903 1101 66-67 -0.16256311367326504 1101 68-69 -0.17884349481632 1101 70-71 -0.20257714090357837 1101 72-73 -0.30228326929437443 1101 74-75 -0.1491387479092765 1101 76-77 -0.09944899495137705 1101 78-79 -0.020529985380917992 1101 80-81 0.026366522373738377 1101 82-83 0.04564998115215246 1101 84-85 0.11097516196054968 1101 86-87 0.03408397248480455 1101 88-89 0.14993480301806628 1101 90-91 0.253287018023979 1101 92-93 0.13779060620865735 1101 94-95 0.09245602806490893 1101 96-97 0.04380976334771702 1101 98-99 0.22543885762238958 1101 100-101 0.4476071480664565 1102 1 0.009574749119245496 1102 2 0.025631831991674403 1102 3 0.11584978118473543 1102 4 -0.007803960705864199 1102 5 -0.03269808203895508 1102 6 0.04265338245683381 1102 7 -0.05262827461467623 1102 8 0.008271532142394733 1102 9 0.07092502995703853 1102 10-11 0.21361965647597714 1102 12-13 0.07058516512466895 1102 14-15 0.21740768706443703 1102 16-17 0.24390196412689136 1102 18-19 0.22888119251074102 1102 20-21 0.26411069686253086 1102 22-23 0.18162401377960435 1102 24-25 0.11883094972260011 1102 26-27 0.09801856408545717 1102 28-29 0.10937162854302329 1102 30-31 0.14613626567614801 1102 32-33 0.143692863634449 1102 34-35 0.05092960001608304 1102 36-37 0.17315367214806088 1102 38-39 0.20597027390294897 1102 40-41 0.06461887217121642 1102 42-43 0.20007945221609447 1102 44-45 0.10585002204265237 1102 46-47 -0.02036648251432638 1102 48-49 0.08974946169502829 1102 50-51 0.09923129815805964 1102 52-53 0.1963225374133728 1102 54-55 0.2509089945787437 1102 56-57 0.26899863450892525 1102 58-59 0.035271212490229686 1102 60-61 -0.006297571421939097 1102 62-63 -0.12899843322405502 1102 64-65 0.21347070135163193 1102 66-67 0.1209447513996551 1102 68-69 0.08633651130309516 1102 70-71 0.08592200095952052 1102 72-73 -0.030912165104194145 1102 74-75 0.13403781755734556 1102 76-77 0.11770016687075113 1102 78-79 0.06069160846399768 1102 80-81 0.02912355311874748 1102 82-83 0.05790578700045046 1102 84-85 0.10124437581799484 1102 86-87 -0.05153392660964329 1102 88-89 0.13433991252930255 1102 90-91 0.3298357170398063 1102 92-93 0.23775798282234817 1102 94-95 0.30290954379201906 1102 96-97 0.4123592992243239 1102 98-99 0.5242125931622255 1102 100-101 0.7461412739522046 1103 1 -0.010539348422572203 1103 2 0.017270889000158718 1103 3 0.02358031763451862 1103 4 0.06330811268715308 1103 5 0.050650524722307466 1103 6 0.06297996527070637 1103 7 0.10789729914591106 1103 8 0.12016214754405041 1103 9 0.1040787746323204 1103 10-11 0.06910071910373716 1103 12-13 0.11385814324170695 1103 14-15 0.22563721143981752 1103 16-17 0.06581841008413392 1103 18-19 0.13727058435976147 1103 20-21 0.15745517476304371 1103 22-23 0.02784207804884886 1103 24-25 0.06689642741164192 1103 26-27 0.14425752441818673 1103 28-29 0.19533123620054482 1103 30-31 0.02817622499176764 1103 32-33 0.17948731686251307 1103 34-35 0.022189959931900205 1103 36-37 0.012340840454527324 1103 38-39 0.06731305997760728 1103 40-41 0.11342880359185159 1103 42-43 0.384350255128453 1103 44-45 0.361609279250537 1103 46-47 0.13291948640844709 1103 48-49 0.36202844176572313 1103 50-51 0.35192210691124615 1103 52-53 0.16624932566324446 1103 54-55 0.10138356180365093 1103 56-57 0.13280413495706256 1103 58-59 0.06354006174653648 1103 60-61 0.15318848612028546 1103 62-63 0.21841070666376083 1103 64-65 0.20979777761471752 1103 66-67 0.20523645037550153 1103 68-69 0.09529362150339438 1103 70-71 0.12593015797707352 1103 72-73 0.17306236687432985 1103 74-75 0.32848953506270817 1103 76-77 0.26493282313806077 1103 78-79 0.19383125459042105 1103 80-81 0.2210911566675584 1103 82-83 0.25535269085841605 1103 84-85 0.173563889216652 1103 86-87 0.29785505235330945 1103 88-89 0.427027800868796 1103 90-91 0.5136860810844759 1103 92-93 0.5129279206880284 1103 94-95 0.4701160673919844 1103 96-97 0.2721598047580045 1103 98-99 0.37170431026477146 1103 100-101 0.7857559304007786 1104 1 -0.030106898187284514 1104 2 0.05587752695657855 1104 3 8.448694183016414E-5 1104 4 0.022216357985143986 1104 5 -0.034481191455405735 1104 6 0.005240423470340261 1104 7 -0.011087894947699795 1104 8 -0.031940470810880583 1104 9 -0.009972614388253476 1104 10-11 0.04568423249991582 1104 12-13 0.03168760834278572 1104 14-15 0.009795839735218692 1104 16-17 0.11066403842444572 1104 18-19 0.06523438549054816 1104 20-21 0.13041331202333595 1104 22-23 0.10470154918805719 1104 24-25 0.03817012459667524 1104 26-27 0.1344899676672 1104 28-29 0.10257974288491312 1104 30-31 0.05190659481506543 1104 32-33 0.05298140098720694 1104 34-35 -0.07812916299156569 1104 36-37 0.05922745825056097 1104 38-39 0.15198991994776634 1104 40-41 0.2113938023692512 1104 42-43 0.1968040334540362 1104 44-45 0.20769571939565168 1104 46-47 0.08831413592214687 1104 48-49 0.296300846490432 1104 50-51 0.27422428313496994 1104 52-53 0.16034293172732816 1104 54-55 0.17998885240761098 1104 56-57 0.15533486207826996 1104 58-59 0.05599364046435085 1104 60-61 0.19125368799859643 1104 62-63 0.012494831358729641 1104 64-65 0.2784793183870917 1104 66-67 0.12803440239976993 1104 68-69 0.16380724223865428 1104 70-71 0.032869023107096496 1104 72-73 -0.10773835969795087 1104 74-75 -0.24152164297245093 1104 76-77 -0.009647875788548532 1104 78-79 0.14294399804952462 1104 80-81 0.055664470106645325 1104 82-83 0.028482740311240207 1104 84-85 0.1835445272628924 1104 86-87 0.4547460116183508 1104 88-89 0.60536935970568 1104 90-91 0.49339744824800036 1104 92-93 0.6179642742532714 1104 94-95 0.5599984485130776 1104 96-97 0.7482535305867515 1104 98-99 0.757069817043952 1104 100-101 1.1801037868628335 1105 1 0.028818541222111804 1105 2 -0.04365999330922676 1105 3 -0.007378010351189346 1105 4 0.012454631134005467 1105 5 0.09086535131104512 1105 6 -0.013010991477671041 1105 7 0.0010852427934153752 1105 8 0.029842178346392245 1105 9 0.07591536426022572 1105 10-11 0.035959818135246735 1105 12-13 0.04352810404770224 1105 14-15 0.10238884781422541 1105 16-17 -0.00900807992978514 1105 18-19 -0.004157084436080538 1105 20-21 0.19844104715052424 1105 22-23 0.07209897155369305 1105 24-25 -0.047181822383500105 1105 26-27 0.056792201734033654 1105 28-29 0.05555820602018002 1105 30-31 0.008416818924096958 1105 32-33 0.12983902629375876 1105 34-35 0.14078514607873416 1105 36-37 0.20595503125133519 1105 38-39 0.17454543995214067 1105 40-41 0.12599161766220135 1105 42-43 0.23069864738442192 1105 44-45 0.11727463945035765 1105 46-47 0.24129337944928153 1105 48-49 0.13727673628349635 1105 50-51 0.1376081500937687 1105 52-53 0.18640338673480983 1105 54-55 0.12781014461949525 1105 56-57 0.2187434089300737 1105 58-59 0.36613234536501693 1105 60-61 0.32645536076857695 1105 62-63 0.1380474463543564 1105 64-65 0.2094075652562637 1105 66-67 0.2375443553226333 1105 68-69 0.13438939506669811 1105 70-71 0.14677258227933265 1105 72-73 0.09282942416471585 1105 74-75 0.12426729419234306 1105 76-77 0.18214684017807414 1105 78-79 0.0471916222440143 1105 80-81 0.28480288821329935 1105 82-83 0.1913558895631411 1105 84-85 0.17347478949406892 1105 86-87 0.36379328970904723 1105 88-89 0.38941883264851995 1105 90-91 0.4067439539458384 1105 92-93 0.47142472691957593 1105 94-95 0.2113378936154362 1105 96-97 0.15596438759359543 1105 98-99 0.17881797288040602 1105 100-101 0.48907458654989355 1106 1 -0.06673414910563835 1106 2 -0.1170961751423647 1106 3 -0.15388881967813717 1106 4 -0.07951408006825034 1106 5 -0.13591912978196063 1106 6 -0.12320950202683179 1106 7 -0.0643015725250109 1106 8 -0.1533979606894249 1106 9 -0.10520327338772262 1106 10-11 -0.12666144125990542 1106 12-13 -0.03940892236872173 1106 14-15 0.03152228432126947 1106 16-17 -0.09897341662487236 1106 18-19 -0.18375298528984985 1106 20-21 -0.12584545692509153 1106 22-23 -0.0775847640486873 1106 24-25 -0.11727075709902834 1106 26-27 -0.12860435068272835 1106 28-29 -0.13855582324468685 1106 30-31 -0.11465991285423627 1106 32-33 -0.1724703781363388 1106 34-35 -0.10363634784848585 1106 36-37 -0.17794848347769232 1106 38-39 -0.19188629610171404 1106 40-41 -0.226978197332933 1106 42-43 -0.1778446042707813 1106 44-45 -0.2878316938721355 1106 46-47 -0.3718162174271882 1106 48-49 -0.2131911322484612 1106 50-51 -0.2094000833976395 1106 52-53 -0.2864711902561794 1106 54-55 -0.35666764848343036 1106 56-57 -0.46480782944136934 1106 58-59 -0.36620481649080716 1106 60-61 -0.28971860043223785 1106 62-63 -0.37112460377353074 1106 64-65 -0.33440913817161544 1106 66-67 -0.2891451460193295 1106 68-69 -0.2737600206117534 1106 70-71 -0.26873082953105865 1106 72-73 -0.2637700424948086 1106 74-75 -0.24949165619053915 1106 76-77 -0.32400651816436365 1106 78-79 -0.3279791117495563 1106 80-81 -0.39005048141575216 1106 82-83 -0.22693043851777617 1106 84-85 -0.2871131571642316 1106 86-87 -0.21937520800397792 1106 88-89 -0.14755201153304043 1106 90-91 -0.11247270701375456 1106 92-93 -0.05192996171326669 1106 94-95 -0.08470624279576455 1106 96-97 -0.178696717385332 1106 98-99 -0.0776613104385504 1106 100-101 0.5650861256809776 1107 1 -0.1661571967001052 1107 2 -0.11023773847455942 1107 3 -0.15373396462013034 1107 4 -0.049051274610782514 1107 5 -0.02115064982513104 1107 6 -0.12542228695427582 1107 7 -0.03553794938590471 1107 8 0.049530676938012164 1107 9 -0.0694043034084828 1107 10-11 -0.08583238012734995 1107 12-13 -0.12378791839176273 1107 14-15 -0.04867773679532661 1107 16-17 -0.09690000124964371 1107 18-19 -0.046170718540274436 1107 20-21 -0.11401664215285479 1107 22-23 -0.2300982258804396 1107 24-25 -0.07537577698382592 1107 26-27 -0.04296352056577035 1107 28-29 -0.1949424212432973 1107 30-31 -0.24824140532851402 1107 32-33 -0.076648179898406 1107 34-35 -0.20982069575618567 1107 36-37 -0.11713666756751451 1107 38-39 -0.05810196696432968 1107 40-41 -0.10930958715707817 1107 42-43 -0.1337529113061322 1107 44-45 -0.2311309278088558 1107 46-47 -0.17663854471100393 1107 48-49 -0.28755804488439907 1107 50-51 -0.2530194684353688 1107 52-53 -0.12064054537871272 1107 54-55 -0.1757260828049354 1107 56-57 -0.16131162456343162 1107 58-59 -0.005222425387934493 1107 60-61 -0.226402238587589 1107 62-63 -0.14865231930947687 1107 64-65 -0.054848949999261265 1107 66-67 -0.1604607101258111 1107 68-69 0.07596323462926335 1107 70-71 -0.001964132753691672 1107 72-73 -0.012224307461199402 1107 74-75 0.0703627565920435 1107 76-77 0.028547886406208534 1107 78-79 0.07760857492704787 1107 80-81 -0.005232025508384197 1107 82-83 0.07705215201964677 1107 84-85 -0.09460708614206936 1107 86-87 -0.02863765213297853 1107 88-89 0.13249550081430428 1107 90-91 0.3512722044725045 1107 92-93 0.356997667009999 1107 94-95 0.40278604377994753 1107 96-97 0.31462938155766906 1107 98-99 0.3382950955163331 1107 100-101 0.7363565237288654 1108 1 -0.007614346052232435 1108 2 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quality scores pass #Quality Count 2 3.0 3 7.0 4 26.0 5 65.0 6 171.0 7 458.0 8 952.0 9 1842.0 10 2933.0 11 4691.0 12 6497.0 13 8884.0 14 10069.0 15 12974.0 16 16556.0 17 22447.0 18 20890.0 19 13911.0 20 12258.0 21 12899.0 22 13948.0 23 15165.0 24 17550.0 25 20658.0 26 24428.0 27 29897.0 28 36310.0 29 44259.0 30 54172.0 31 65403.0 32 79526.0 33 100049.0 34 131362.0 35 182558.0 36 273425.0 37 435817.0 38 390252.0 39 40814.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.74144758814829 20.32725797986545 15.133975857626794 22.797318574359462 2 16.095646272470116 21.9251395352374 38.639949660857134 23.33926453143535 3 18.199130471150042 25.808648523283757 31.507683943182162 24.48453706238404 4 13.35888310977279 18.316898971925664 36.95725735316483 31.36696056513672 5 13.409355324390912 37.5065585363628 34.41344823128631 14.670637907959971 6 30.258425342944918 36.89575919335706 17.925477917693218 14.920337546004806 7 26.6011858594154 33.979064011314904 21.787220169115187 17.632529960154518 8 25.528722587219026 34.77549844876358 20.86161108069471 18.83416788332269 9 26.15249642607294 16.682160020683153 21.204080740335186 35.96126281290872 10-11 24.21157363050157 26.957509238981658 28.013053388387014 20.817863742129756 12-13 25.160446512759684 24.872369931867265 28.29977548894364 21.66740806642942 14-15 22.538727499554447 26.2141032495693 26.752545594962278 24.494623655913976 16-17 21.411923501007564 29.126530360062354 27.56166590623931 21.899880232690773 18-19 21.253760170788212 27.439058440640334 30.24732590935003 21.059855479221426 20-21 22.2976453903945 26.68067246859507 30.041328284210845 20.980353856799585 22-23 22.07163727681966 26.693338047875415 29.915765580801168 21.319259094503757 24-25 21.89821626821182 26.83712682878608 30.0537324725492 21.210924430452902 26-27 21.60506148636607 27.09323352937682 29.985956157547673 21.31574882670944 28-29 21.47706793503057 27.033526477476656 30.10049768835356 21.388907899139216 30-31 21.75689881984366 26.91654214952702 29.900723720229948 21.425835310399364 32-33 21.704097849560483 26.950641786051037 29.717536195516626 21.627724168871858 34-35 21.921857336132184 26.950875242608046 29.64120744719658 21.486059974063192 36-37 22.00660724896019 26.883469994058228 29.509708853238266 21.600213903743317 38-39 21.708296269080414 26.87132392521192 29.709992174026084 21.710387631681577 40-41 21.853538345713496 26.688373244776958 29.731833217243302 21.72625519226624 42-43 21.79468564651187 26.84842350953979 29.364040366786732 21.992850477161614 44-45 21.753966980935914 26.99317400060596 29.240252603563306 22.012606414894815 46-47 21.96536324770584 27.057244814812265 28.83537312606444 22.14201881141745 48-49 22.037252486540744 27.162035769687016 28.50453964777808 22.29617209599416 50-51 21.853516840064653 27.37687364640498 28.4652151327943 22.30439438073607 52-53 21.981409876205248 27.40555707637558 28.32926513976336 22.283767907655808 54-55 21.79235293670347 27.32825664598351 28.202942026340605 22.67644839097241 56-57 21.78275276028835 27.57907313623506 28.07897143139581 22.559202672080787 58-59 21.739074643591927 27.32226646876911 28.272640473281218 22.666018414357744 60-61 21.784817318639266 27.337984696175045 28.367610222732655 22.509587762453044 62-63 21.4755233521915 27.327519998783345 28.405116038567996 22.791840610457157 64-65 21.4215580040758 27.379109065608176 28.243980404233966 22.955352526082063 66-67 21.399950477709503 27.48667722064937 28.05133588992554 23.062036411715585 68-69 21.342322068898326 27.27296274205545 28.167471834951986 23.21724335409424 70-71 21.392109946416 27.235187643812885 28.04031406607973 23.332388343691385 72-73 21.443918811022904 27.28600636463181 27.80541392919062 23.46466089515467 74-75 21.32384531739514 27.39244000364997 27.599390341272013 23.68432433768288 76-77 21.340124551118123 27.246562070875825 27.873575453425108 23.539737924580947 78-79 21.218892351270075 27.276984446383633 27.801777264112438 23.702345938233858 80-81 21.16756232690272 27.269568978115633 27.689054014074717 23.873814680906932 82-83 21.224207842868875 27.206971248897403 27.65554186817532 23.9132790400584 84-85 21.109837424339204 26.977113559631356 27.677569995741703 24.23547902028774 86-87 21.11402669144249 26.970765450103357 27.669536924082315 24.245670934371834 88-89 21.145133636193727 26.831823799334014 27.54670687004848 24.476335694423785 90-91 20.973540823022947 26.887597466316603 27.63708907590899 24.501772634751458 92-93 20.979403344283238 26.785965492593604 27.550510235118775 24.68412092800438 94-95 20.883506556400825 26.60252921995678 27.591062706766273 24.92290151687612 96-97 20.981328132128844 26.497294841378473 27.499586527055385 25.021790499437298 98-99 20.80699616562522 26.457423611916408 27.452754685560354 25.282825536898017 100-101 20.77044451098089 26.240999757617235 27.811449971722013 25.17710575967986 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1279.0 1 911.5 2 613.5 3 711.5 4 649.5 5 655.0 6 997.5 7 1750.5 8 2657.0 9 3132.0 10 2974.0 11 2749.0 12 2829.0 13 3005.5 14 3244.0 15 3476.0 16 3716.5 17 4043.0 18 4290.5 19 4587.5 20 5120.0 21 5853.5 22 6666.0 23 7649.0 24 8865.5 25 10639.5 26 12982.5 27 15738.0 28 18379.0 29 21365.5 30 25408.0 31 29852.0 32 34410.5 33 38380.5 34 41940.5 35 45196.0 36 49461.0 37 54055.0 38 56816.5 39 58228.5 40 59964.5 41 62984.5 42 65880.5 43 69675.0 44 74001.0 45 76950.0 46 78819.0 47 79825.5 48 80887.5 49 80707.0 50 78740.5 51 75992.0 52 73467.0 53 72021.5 54 69241.0 55 63995.0 56 57490.5 57 50855.5 58 45967.0 59 40959.0 60 34303.5 61 27731.0 62 22181.0 63 17149.5 64 12608.5 65 9380.0 66 7103.0 67 5564.0 68 4064.5 69 2846.5 70 2142.5 71 1563.0 72 1223.0 73 993.5 74 733.5 75 554.0 76 384.5 77 208.5 78 111.5 79 68.0 80 44.0 81 29.0 82 24.0 83 15.5 84 7.0 85 6.5 86 5.5 87 5.0 88 6.0 89 3.5 90 1.0 91 2.0 92 1.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3910408492259026 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.4257687745232228E-4 16-17 0.0022812300392371565 18-19 7.128843872616114E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.4257687745232228E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008008067950238769 36-37 0.01796468655899261 38-39 0.01123981050582474 40-41 0.00748528606624692 42-43 0.0033980822459470147 44-45 0.001924787845606351 46-47 0.0015921084648842655 48-49 0.0 50-51 4.2773063235696684E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.2773063235696684E-4 60-61 6.415959485354503E-4 62-63 0.0 64-65 0.0 66-67 0.0011881406454360192 68-69 6.178331356267299E-4 70-71 0.002304992852145877 72-73 0.0 74-75 0.0 76-77 0.09978005140371687 78-79 0.03452736715637072 80-81 2.3762812908720383E-5 82-83 0.0 84-85 0.0 86-87 0.1499433494540256 88-89 0.3227940505520577 90-91 0.2775021291480366 92-93 0.0 94-95 4.7525625817440767E-5 96-97 0.0 98-99 1.9010250326976307E-4 100-101 8.554612647139337E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2104128.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.887333271671345 #Duplication Level Percentage of deduplicated Percentage of total 1 78.80906594034658 44.8323759897762 2 12.06799815037342 13.730324654044123 3 3.6842064715312524 6.287540441629402 4 1.6185294217250654 3.6829529049471725 5 0.9312169291906887 2.6487223899546546 6 0.5657751182183548 1.931126262414409 7 0.3897071441171109 1.5518580130018942 8 0.28461064462028557 1.295259247454352 9 0.19923323213739175 1.020046254785286 >10 1.3143987777331587 13.956091673431361 >50 0.0953345036112775 3.695291388864925 >100 0.03841819908323338 3.552609624769662 >500 7.527336561364665E-4 0.28238077388348803 >1k 5.018224374243079E-4 0.438249251599123 >5k 2.5091121871215395E-4 1.0951711294439923 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9478 0.45044788149770354 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8292 0.3940824892782188 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5248 0.24941448428992913 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 2197 0.10441379992091736 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 9.505125163488153E-5 3 0.0 0.0 0.0 0.0 9.505125163488153E-5 4 0.0 0.0 0.0 0.0 9.505125163488153E-5 5 0.0 0.0 0.0 0.0 9.505125163488153E-5 6 0.0 0.0 0.0 0.0 9.505125163488153E-5 7 0.0 0.0 0.0 0.0 9.505125163488153E-5 8 0.0 0.0 0.0 0.0 9.505125163488153E-5 9 0.0 0.0 0.0 0.0 9.505125163488153E-5 10-11 0.0 0.0 0.0 0.0 1.4257687745232228E-4 12-13 0.0 4.7525625817440767E-5 0.0 9.505125163488153E-5 1.4257687745232228E-4 14-15 0.0 4.7525625817440767E-5 0.0 9.505125163488153E-5 1.9010250326976307E-4 16-17 0.0 4.7525625817440767E-5 0.0 1.4257687745232228E-4 1.9010250326976307E-4 18-19 2.3762812908720383E-5 4.7525625817440767E-5 0.0 1.4257687745232228E-4 1.9010250326976307E-4 20-21 4.7525625817440767E-5 4.7525625817440767E-5 0.0 3.802050065395261E-4 1.9010250326976307E-4 22-23 4.7525625817440767E-5 4.7525625817440767E-5 0.0 0.001306954709979621 1.9010250326976307E-4 24-25 9.505125163488153E-5 4.7525625817440767E-5 0.0 0.002613909419959242 1.9010250326976307E-4 26-27 9.505125163488153E-5 4.7525625817440767E-5 0.0 0.005370395717370807 1.9010250326976307E-4 28-29 9.505125163488153E-5 4.7525625817440767E-5 0.0 0.014780469629224078 2.1386531617848345E-4 30-31 9.505125163488153E-5 4.7525625817440767E-5 0.0 0.041394820086990906 2.3762812908720383E-4 32-33 9.505125163488153E-5 4.7525625817440767E-5 0.0 0.09398192505398911 2.3762812908720383E-4 34-35 1.4257687745232228E-4 4.7525625817440767E-5 0.0 0.18085876904827083 2.8515375490464456E-4 36-37 1.4257687745232228E-4 4.7525625817440767E-5 0.0 0.3125998038142166 2.8515375490464456E-4 38-39 1.9010250326976307E-4 4.7525625817440767E-5 0.0 0.4998032439091158 2.8515375490464456E-4 40-41 1.9010250326976307E-4 4.7525625817440767E-5 0.0 0.7000524682909024 2.8515375490464456E-4 42-43 1.9010250326976307E-4 4.7525625817440767E-5 0.0 0.9040799799251757 2.8515375490464456E-4 44-45 1.9010250326976307E-4 4.7525625817440767E-5 0.0 1.1538746692216444 2.8515375490464456E-4 46-47 1.9010250326976307E-4 4.7525625817440767E-5 0.0 1.450244471819205 3.3267938072208537E-4 48-49 1.9010250326976307E-4 4.7525625817440767E-5 0.0 1.7604679943425494 3.3267938072208537E-4 50-51 1.9010250326976307E-4 7.128843872616115E-5 0.0 2.0839036408431424 3.3267938072208537E-4 52-53 1.9010250326976307E-4 9.505125163488153E-5 0.0 2.400614411290568 3.564421936308057E-4 54-55 1.9010250326976307E-4 9.505125163488153E-5 0.0 2.7609061806125865 3.8020500653952613E-4 56-57 1.9010250326976307E-4 9.505125163488153E-5 0.0 3.171575113301092 3.8020500653952613E-4 58-59 1.9010250326976307E-4 9.505125163488153E-5 0.0 3.6171516181525076 3.8020500653952613E-4 60-61 1.9010250326976307E-4 9.505125163488153E-5 0.0 4.103006090884205 3.8020500653952613E-4 62-63 1.9010250326976307E-4 9.505125163488153E-5 0.0 4.589478396751529 3.8020500653952613E-4 64-65 1.9010250326976307E-4 9.505125163488153E-5 0.0 5.098050118623962 3.8020500653952613E-4 66-67 1.9010250326976307E-4 9.505125163488153E-5 0.0 5.667953660613803 3.8020500653952613E-4 68-69 1.9010250326976307E-4 1.425768774523223E-4 0.0 6.304868335006235 3.8020500653952613E-4 70-71 1.9010250326976307E-4 1.9010250326976307E-4 0.0 6.990235384919549 4.277306323569669E-4 72-73 1.9010250326976307E-4 1.9010250326976307E-4 0.0 7.67396280074216 4.7525625817440765E-4 74-75 1.9010250326976307E-4 1.9010250326976307E-4 0.0 8.389532385862458 4.7525625817440765E-4 76-77 1.9010250326976307E-4 1.9010250326976307E-4 0.0 9.153174141497095 4.7525625817440765E-4 78-79 1.9010250326976307E-4 1.9010250326976307E-4 4.7525625817440767E-5 9.980666575417466 4.7525625817440765E-4 80-81 1.9010250326976307E-4 1.9010250326976307E-4 4.7525625817440767E-5 10.871082937920127 4.7525625817440765E-4 82-83 1.9010250326976307E-4 1.9010250326976307E-4 4.7525625817440767E-5 11.783931395808619 4.7525625817440765E-4 84-85 1.9010250326976307E-4 1.9010250326976307E-4 4.7525625817440767E-5 12.69704124463911 4.7525625817440765E-4 86-87 1.9010250326976307E-4 1.9010250326976307E-4 4.7525625817440767E-5 13.622388942117588 4.7525625817440765E-4 88-89 1.9010250326976307E-4 1.9010250326976307E-4 4.7525625817440767E-5 14.601535648021414 4.7525625817440765E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5465 0.0 56.663517 1 GTATCAA 9270 0.0 39.31534 1 ATCAACG 12140 0.0 29.74537 3 TCAACGC 12235 0.0 29.51441 4 TATCAAC 12385 0.0 29.348516 2 CAACGCA 12380 0.0 29.168724 5 AACGCAG 12765 0.0 28.288977 6 GTGGTAT 2260 0.0 27.19328 1 ACGCAGA 14420 0.0 24.680243 7 CGCAGAG 14505 0.0 24.372046 8 GTACATG 8855 0.0 23.672518 1 TGGTATC 2555 0.0 23.215204 2 GCAGAGT 15550 0.0 22.337477 9 TACATGG 9125 0.0 22.308884 2 AATACCG 150 3.058599E-5 22.14421 5 GAGTACT 9125 0.0 22.050968 12-13 ACATGGG 9180 0.0 21.658321 3 CAGAGTA 15015 0.0 20.986126 10-11 AGTACTT 9420 0.0 20.378035 12-13 AGAGTAC 14005 0.0 20.042929 10-11 >>END_MODULE