FastQCFastQC Report
Thu 26 May 2016
SRR938796_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938796_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103102
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99480.473015574137631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82650.3929909248338882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51150.2432121694525515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48400.055.3895531
GTATCAA87200.042.6269651
ATCAACG113600.032.820653
TCAACGC115050.032.4074
TATCAAC115350.032.3227162
CAACGCA116800.031.8402275
AACGCAG120000.030.9896766
ACGCAGA136800.026.8725057
CGCAGAG136450.026.5242798
GTGGTAT20400.024.9314611
CGTATAC1000.001667245223.717723
GCAGAGT147050.023.7073969
GAGTACT90550.022.40806212-13
GTACATG83800.021.9513421
TGGTATC23350.021.1275622
TACATGG86250.021.0090892
CAGAGTA142200.020.8922310-11
AGTACTT93100.020.77468912-13
AGAGTAC134050.020.40997710-11
GTACTTT99850.020.20214514-15
Produced by FastQC (version 0.11.5)