##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938796_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2103102 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.590526755240592 33.0 31.0 34.0 30.0 34.0 2 31.658156380432334 33.0 31.0 34.0 30.0 34.0 3 31.65865754490272 33.0 31.0 34.0 28.0 34.0 4 35.22632140523855 37.0 35.0 37.0 33.0 37.0 5 35.26196922450742 37.0 35.0 37.0 33.0 37.0 6 35.321019617688535 37.0 35.0 37.0 33.0 37.0 7 35.240636450348106 37.0 35.0 37.0 33.0 37.0 8 35.21355692686327 37.0 35.0 37.0 32.0 37.0 9 36.79261110492977 39.0 37.0 39.0 33.0 39.0 10-11 36.6819108155477 39.0 37.0 39.0 32.0 39.0 12-13 36.57592047366224 39.0 37.0 39.0 32.0 39.0 14-15 37.73888380116608 40.0 37.5 41.0 32.0 41.0 16-17 37.624632091073096 40.0 37.0 41.0 32.0 41.0 18-19 37.584090548152204 40.0 37.0 41.0 32.0 41.0 20-21 37.489749189530514 40.0 37.0 41.0 32.0 41.0 22-23 37.46981292395709 40.0 37.0 41.0 32.0 41.0 24-25 37.40067909212202 40.0 37.0 41.0 32.0 41.0 26-27 37.268439666739894 40.0 37.0 41.0 31.0 41.0 28-29 37.09872417029702 40.0 36.5 41.0 31.0 41.0 30-31 36.85517107586793 39.5 36.0 41.0 30.0 41.0 32-33 36.64923123081999 39.0 35.5 41.0 30.0 41.0 34-35 36.34762745696595 39.0 35.0 41.0 30.0 41.0 36-37 36.13334517298733 39.0 35.0 41.0 29.5 41.0 38-39 35.96413844882464 39.0 35.0 41.0 29.0 41.0 40-41 35.8332532135864 39.0 35.0 41.0 28.0 41.0 42-43 35.802272310139976 39.0 35.0 41.0 27.5 41.0 44-45 35.598762922578175 39.0 35.0 41.0 27.0 41.0 46-47 35.245853743660554 39.0 35.0 41.0 25.5 41.0 48-49 34.92039615767567 39.0 35.0 41.0 24.0 41.0 50-51 33.761462116435624 38.0 33.0 40.0 22.0 40.5 52-53 33.81556458031993 38.0 33.5 39.5 22.5 40.5 54-55 34.307933471605274 38.0 34.0 40.0 23.0 41.0 56-57 34.205962430733265 38.0 34.0 40.0 22.0 41.0 58-59 33.99860444239034 38.0 34.0 40.0 21.5 41.0 60-61 33.6996888405793 38.0 33.5 40.0 20.0 41.0 62-63 33.30927767649881 37.0 33.0 40.0 18.5 41.0 64-65 32.91615290176131 36.5 32.5 39.5 16.0 41.0 66-67 32.53810181341656 36.0 32.0 39.0 13.0 41.0 68-69 32.115017959186005 35.5 32.0 39.0 9.5 40.5 70-71 31.703883834450252 35.0 31.0 38.5 7.5 40.0 72-73 31.27869713404295 35.0 31.0 37.5 4.5 40.0 74-75 30.80758208589027 35.0 31.0 37.0 2.0 39.0 76-77 30.3410191231809 35.0 30.0 36.5 2.0 39.0 78-79 29.89709961761246 34.5 30.0 36.0 2.0 38.5 80-81 29.425267295642342 34.0 29.0 35.5 2.0 37.0 82-83 29.004757020819724 34.0 29.0 35.0 2.0 37.0 84-85 28.647450527839354 34.0 29.0 35.0 2.0 36.5 86-87 28.19512153951639 34.0 28.5 35.0 2.0 36.0 88-89 27.73960559212059 34.0 27.0 35.0 2.0 36.0 90-91 27.27244208792536 34.0 26.0 35.0 2.0 35.0 92-93 26.79731344461657 33.5 25.0 35.0 2.0 35.0 94-95 26.270067975780535 33.0 24.5 35.0 2.0 35.0 96-97 25.664987718142058 33.0 21.5 35.0 2.0 35.0 98-99 24.900318672132876 32.5 10.0 35.0 2.0 35.0 100-101 22.507412859671096 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13664725322790972 1101 2 0.18277537581856862 1101 3 0.26496247395120065 1101 4 0.15898513609116094 1101 5 0.15945385637387943 1101 6 0.2379419044707376 1101 7 0.2957122483773986 1101 8 0.31369815976196236 1101 9 0.4245338327640198 1101 10-11 0.27637621291726333 1101 12-13 0.2381302274730075 1101 14-15 0.25089693150587067 1101 16-17 0.44697053770221373 1101 18-19 0.4191684462454859 1101 20-21 0.5485494214558599 1101 22-23 0.4910590908964849 1101 24-25 0.3550663243055041 1101 26-27 0.44782838615528675 1101 28-29 0.5615061509956618 1101 30-31 0.6160616906721543 1101 32-33 0.47263706428252306 1101 34-35 0.25354036482264775 1101 36-37 0.30485056726550397 1101 38-39 0.31123571784223003 1101 40-41 0.4270512854377486 1101 42-43 0.4870076961321672 1101 44-45 0.6820940264418951 1101 46-47 0.41765786101391456 1101 48-49 0.4771259623951636 1101 50-51 0.44286281790422777 1101 52-53 0.5111255476188958 1101 54-55 0.5892464970874656 1101 56-57 0.5888779699436597 1101 58-59 0.5674435563978548 1101 60-61 0.47699779676000986 1101 62-63 0.31405030663969313 1101 64-65 0.22189694357902567 1101 66-67 0.16755512810136253 1101 68-69 0.23753813462158746 1101 70-71 -0.004474398236755661 1101 72-73 -0.013234545884532878 1101 74-75 -0.07823471069740862 1101 76-77 0.06375089760188857 1101 78-79 -0.07239550467703992 1101 80-81 -0.1800189108179282 1101 82-83 -0.1974417704097604 1101 84-85 -0.22355641914274216 1101 86-87 -0.48985813744604556 1101 88-89 -0.31864025068174584 1101 90-91 -0.3886531830289357 1101 92-93 -0.40160372099753516 1101 94-95 -0.26642522975107497 1101 96-97 -0.3796094500939944 1101 98-99 -0.2933482930302489 1101 100-101 -0.0038077445166067037 1102 1 0.20077183931807951 1102 2 0.15453891379526397 1102 3 0.11294162044325873 1102 4 0.2751195864432461 1102 5 0.28391677848568975 1102 6 0.21969901853247364 1102 7 0.2202736557679259 1102 8 0.1823021788758794 1102 9 0.39142891349072073 1102 10-11 0.39899456426976343 1102 12-13 0.3340298373916539 1102 14-15 0.4647844129353942 1102 16-17 0.5791174504001546 1102 18-19 0.5400061463905033 1102 20-21 0.4861303810997626 1102 22-23 0.4191143164058957 1102 24-25 0.3017577868413923 1102 26-27 0.38278421897418724 1102 28-29 0.4440066494452495 1102 30-31 0.5166724419899396 1102 32-33 0.5402462493254419 1102 34-35 0.5593080226602964 1102 36-37 0.48014600466331103 1102 38-39 0.465134576951975 1102 40-41 0.3318943715425533 1102 42-43 0.5201566215584563 1102 44-45 0.5861344304822964 1102 46-47 0.48071572812173 1102 48-49 0.43444183782382595 1102 50-51 0.5016122613739071 1102 52-53 0.4432415085030854 1102 54-55 0.38647991137737137 1102 56-57 0.4590020451691075 1102 58-59 0.35568312097786503 1102 60-61 0.3502155782942964 1102 62-63 0.22765160056809464 1102 64-65 0.3305664507322419 1102 66-67 0.2763060097609511 1102 68-69 0.3167243797240573 1102 70-71 0.318899039698465 1102 72-73 0.2159979302630255 1102 74-75 0.10429015093519922 1102 76-77 0.10117100123514788 1102 78-79 0.006415335980616277 1102 80-81 0.02588120799868676 1102 82-83 -0.03071610107986089 1102 84-85 -0.08830498298103961 1102 86-87 -0.22188598768646983 1102 88-89 -0.19586136291636436 1102 90-91 -0.19908364925077038 1102 92-93 -0.3635143059032302 1102 94-95 -0.5326081237481155 1102 96-97 -0.8138329123555224 1102 98-99 -0.9813326219228955 1102 100-101 -0.5936367179218927 1103 1 0.05541187152503824 1103 2 0.22774747412683283 1103 3 0.2972279843297585 1103 4 0.3336619608954763 1103 5 0.3561166991268152 1103 6 0.30227207069211204 1103 7 0.2588677003768822 1103 8 0.30076050243416574 1103 9 0.4024920906840279 1103 10-11 0.27778764324921923 1103 12-13 0.39369887613557353 1103 14-15 0.46461135845871837 1103 16-17 0.5130797896911972 1103 18-19 0.6351375953850535 1103 20-21 0.4951165425342481 1103 22-23 0.4028718142264225 1103 24-25 0.44310165984402516 1103 26-27 0.3775502261512358 1103 28-29 0.5304372234647659 1103 30-31 0.5260532255574404 1103 32-33 0.44078155967946486 1103 34-35 0.48832817228363723 1103 36-37 0.5933192879484182 1103 38-39 0.6116298979644128 1103 40-41 0.6002182339446946 1103 42-43 0.6116166365613367 1103 44-45 0.6723441609227976 1103 46-47 0.6557060254857916 1103 48-49 0.5984558472715804 1103 50-51 0.7330400404550161 1103 52-53 0.667537488136233 1103 54-55 0.6803050525000387 1103 56-57 0.6147043711950531 1103 58-59 0.6712718537556839 1103 60-61 0.6233849660434814 1103 62-63 0.42722106420059447 1103 64-65 0.3217294492232625 1103 66-67 0.2912947720811303 1103 68-69 0.3101823412436957 1103 70-71 0.25436456348150926 1103 72-73 0.4438936602343233 1103 74-75 0.40378373126578637 1103 76-77 0.2188404425660586 1103 78-79 0.10137735088501998 1103 80-81 0.2502097711107112 1103 82-83 0.26074420355394423 1103 84-85 0.19184233778810622 1103 86-87 0.3813040797106453 1103 88-89 0.36092258979269687 1103 90-91 0.20692459943792585 1103 92-93 -0.035060167511780094 1103 94-95 0.096417746085951 1103 96-97 0.1272333494071134 1103 98-99 0.024192449977981312 1103 100-101 0.5443662623803043 1104 1 -0.0040484377680485295 1104 2 0.1272729579332541 1104 3 0.04476821815761767 1104 4 0.15632582620148838 1104 5 0.19736673485845557 1104 6 0.11234410568053477 1104 7 0.17482259275318057 1104 8 0.11456292075163788 1104 9 0.25897238111080156 1104 10-11 0.22046980315545994 1104 12-13 0.22981927747336783 1104 14-15 0.2976016680376503 1104 16-17 0.4419502410532843 1104 18-19 0.46422458049210746 1104 20-21 0.42820618607539274 1104 22-23 0.3658252487517615 1104 24-25 0.24206615688544986 1104 26-27 0.1901785027427323 1104 28-29 0.18733700335786097 1104 30-31 0.24887359545138565 1104 32-33 0.19077084018713464 1104 34-35 0.5243828274018298 1104 36-37 0.5936108581396553 1104 38-39 0.3611410891143976 1104 40-41 0.30982218443492826 1104 42-43 0.2794002743282533 1104 44-45 0.42405713579630344 1104 46-47 0.3713028868184409 1104 48-49 0.26361249478608784 1104 50-51 0.3893881238097947 1104 52-53 0.45972940992681544 1104 54-55 0.3888570634767987 1104 56-57 0.32761278385262216 1104 58-59 0.3373618117352777 1104 60-61 0.21240034494180549 1104 62-63 0.19320311262410428 1104 64-65 0.3784691513642997 1104 66-67 0.3631103682278294 1104 68-69 0.36521764410507984 1104 70-71 0.46594090055951654 1104 72-73 0.3040121463561505 1104 74-75 0.3248353849261356 1104 76-77 0.3681079439238637 1104 78-79 0.21368618660522642 1104 80-81 0.1379638230788096 1104 82-83 0.13897148537654047 1104 84-85 0.04882072729157727 1104 86-87 0.09517438814617307 1104 88-89 0.3259820472434569 1104 90-91 0.21729678021144494 1104 92-93 0.2012253140078144 1104 94-95 0.25860152106237067 1104 96-97 0.19676212052451447 1104 98-99 0.2835134058708455 1104 100-101 0.6333844998099174 1105 1 0.03236035956366479 1105 2 0.13652761957521875 1105 3 0.13349569648232062 1105 4 0.18586304752318483 1105 5 0.25151063192046763 1105 6 0.22023954446420646 1105 7 0.15381391351563423 1105 8 0.23441026465872739 1105 9 0.29024453801646644 1105 10-11 0.40297066378382596 1105 12-13 0.4515484907336571 1105 14-15 0.326517071119504 1105 16-17 0.388878424751276 1105 18-19 0.41645982043073815 1105 20-21 0.3683226387818692 1105 22-23 0.3300447671190554 1105 24-25 0.26920946347660646 1105 26-27 0.4695611529309289 1105 28-29 0.3788856328499435 1105 30-31 0.4789080433839388 1105 32-33 0.5758068497897 1105 34-35 0.44689595340922494 1105 36-37 0.5977904342317402 1105 38-39 0.5626166962848558 1105 40-41 0.4869133973623647 1105 42-43 0.419274155226411 1105 44-45 0.6654695466272358 1105 46-47 0.7718151601768071 1105 48-49 0.6081196340118211 1105 50-51 0.6037988042647555 1105 52-53 0.5944200282797496 1105 54-55 0.5305987928952405 1105 56-57 0.5113824728637084 1105 58-59 0.6287188231776426 1105 60-61 0.582870874974887 1105 62-63 0.5798541397926087 1105 64-65 0.59570816438886 1105 66-67 0.5482208136343516 1105 68-69 0.6906684195825292 1105 70-71 0.5904129535058331 1105 72-73 0.5511210050147568 1105 74-75 0.5812652477043585 1105 76-77 0.5253756369610976 1105 78-79 0.5263207809015995 1105 80-81 0.6883851320090528 1105 82-83 0.5835455370324851 1105 84-85 0.5847783501790467 1105 86-87 0.5599457345792942 1105 88-89 0.6810618064043048 1105 90-91 0.7355635316870526 1105 92-93 0.7016428612511802 1105 94-95 0.795125106839091 1105 96-97 0.4508151671489138 1105 98-99 0.6305576319094683 1105 100-101 0.9277216791831044 1106 1 0.10742247024275642 1106 2 0.10531077583791415 1106 3 0.23563094611305146 1106 4 0.2497497252341816 1106 5 0.2904689463204022 1106 6 0.22426784218814788 1106 7 0.19276872409585621 1106 8 0.1597762769505664 1106 9 0.20875773591986757 1106 10-11 0.1748013767344858 1106 12-13 0.2945406870701959 1106 14-15 0.36165392265280616 1106 16-17 0.43192580624825894 1106 18-19 0.3810184650656723 1106 20-21 0.36795309529672693 1106 22-23 0.359261477273229 1106 24-25 0.2998001089349387 1106 26-27 0.3590975428359826 1106 28-29 0.13539118585671162 1106 30-31 0.16401308680393356 1106 32-33 0.2578645555104515 1106 34-35 0.20203282684140333 1106 36-37 0.44253651132234495 1106 38-39 0.44543275998429976 1106 40-41 0.619550711509369 1106 42-43 0.6464099249553712 1106 44-45 0.6467428347101887 1106 46-47 0.4371087309961226 1106 48-49 0.4769171340364551 1106 50-51 0.4608363903957553 1106 52-53 0.2874892539027414 1106 54-55 0.25227210769482866 1106 56-57 0.32807850226951984 1106 58-59 0.539594570066086 1106 60-61 0.5966780050290055 1106 62-63 0.49705888002269916 1106 64-65 0.4134187685897146 1106 66-67 0.5004053904769492 1106 68-69 0.6541855538083965 1106 70-71 0.6480592188172523 1106 72-73 0.5778131035728293 1106 74-75 0.38128090539868964 1106 76-77 0.4023166709601895 1106 78-79 0.4442717252357582 1106 80-81 0.3883841465597584 1106 82-83 0.4885993589880755 1106 84-85 0.6215467772596348 1106 86-87 0.6249607517315887 1106 88-89 0.8411787025894135 1106 90-91 1.0034193801643667 1106 92-93 1.026237923712241 1106 94-95 1.1483069583669199 1106 96-97 1.3026550557274454 1106 98-99 1.170826922353129 1106 100-101 1.6312683111911532 1107 1 0.03172395833574626 1107 2 0.05677257914793188 1107 3 0.055281590291155425 1107 4 0.16223802443771973 1107 5 0.09428834540420183 1107 6 -0.11430327328238121 1107 7 0.0558410644386953 1107 8 0.07152976073788153 1107 9 -0.12429092751717974 1107 10-11 0.1540157957865702 1107 12-13 0.13417148712936466 1107 14-15 0.18335742905896524 1107 16-17 0.1634507479737053 1107 18-19 0.17953717392395419 1107 20-21 0.07869321183504496 1107 22-23 0.03272120979869442 1107 24-25 -0.05062036397696801 1107 26-27 0.01126695417390522 1107 28-29 0.005405512921903721 1107 30-31 -0.12621784130270441 1107 32-33 -0.15645840698546465 1107 34-35 -0.14674451415462642 1107 36-37 0.004230559922078214 1107 38-39 -0.11333001642171325 1107 40-41 -0.10963018415353787 1107 42-43 -0.0813937586227027 1107 44-45 0.09401923478404228 1107 46-47 0.18782557010221268 1107 48-49 0.03810978933719866 1107 50-51 0.05901673435329258 1107 52-53 -0.018553377483570443 1107 54-55 -0.16278340293494864 1107 56-57 0.09905113401212162 1107 58-59 0.2186588086532879 1107 60-61 0.10127259874820282 1107 62-63 0.23131071463200925 1107 64-65 0.04889040110859355 1107 66-67 -0.020160695945371288 1107 68-69 0.1414902841151573 1107 70-71 0.185623013125074 1107 72-73 -0.10564901499740387 1107 74-75 0.014929642900298745 1107 76-77 -0.1184090969197591 1107 78-79 -0.06803152054088812 1107 80-81 -0.06347702234305785 1107 82-83 -0.07005653124954492 1107 84-85 0.23951542182425456 1107 86-87 0.14045114871113284 1107 88-89 0.17262839213367798 1107 90-91 0.25868643903489286 1107 92-93 0.4770431868303575 1107 94-95 0.3234355186750122 1107 96-97 0.3375512303902397 1107 98-99 0.6488143097852586 1107 100-101 1.091341576801124 1108 1 0.18918609127210928 1108 2 0.24497685108471856 1108 3 0.00999435320472486 1108 4 0.23178432621241285 1108 5 0.2669822287706296 1108 6 0.09825575015995014 1108 7 0.23594354057489397 1108 8 0.10208122796304053 1108 9 0.14626553694616007 1108 10-11 0.3754111107821885 1108 12-13 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sequence quality scores pass #Quality Count 2 11122.0 3 2323.0 4 1956.0 5 2339.0 6 2804.0 7 3368.0 8 4161.0 9 4937.0 10 6078.0 11 7713.0 12 10013.0 13 12465.0 14 13310.0 15 15312.0 16 18474.0 17 22860.0 18 21183.0 19 14657.0 20 14840.0 21 15472.0 22 17028.0 23 19042.0 24 21803.0 25 24546.0 26 28481.0 27 33353.0 28 39202.0 29 46281.0 30 54841.0 31 65990.0 32 80478.0 33 100689.0 34 131702.0 35 182047.0 36 264701.0 37 385907.0 38 347051.0 39 54568.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0445487200074 19.518087339101015 14.605751599335187 22.831612341556397 2 15.932648348000363 21.275124344698792 39.303307714253194 23.48891959304765 3 18.31892865599382 25.539943143568923 31.272132868915293 24.86899533152197 4 13.107553763069571 17.643497970355714 37.29568414229371 31.953264124281006 5 13.558334052238767 37.11429929121485 34.43968510512145 14.887681551424933 6 30.329096218135287 36.35141837197015 18.33156978757889 14.987915622315676 7 26.705947736031977 33.762908464177556 22.045554855431874 17.485588944358597 8 25.53390371405269 34.09633552076198 21.27559169968783 19.094169065497496 9 26.527448937414405 16.540411015004008 21.38404702538891 35.54809302219268 10-11 24.20798320528923 26.644069153346077 28.21538092088837 20.93256672047632 12-13 25.18654285035617 24.829050533284192 28.317775289930914 21.666631326428725 14-15 22.680801274410502 26.01860317349935 26.9446179662064 24.355977585883746 16-17 21.489052765598288 28.858450101449378 27.689219185496196 21.963277947456135 18-19 21.32934647427244 27.287711788979884 30.361450491641396 21.021491245106283 20-21 22.38277132413108 26.567188000624125 30.131054090333585 20.918986584911213 22-23 22.176736182691975 26.459067926781167 30.0226291524018 21.341566738125056 24-25 21.821700835879355 26.700837070845406 30.128878932251524 21.348583161023722 26-27 21.55313309246538 26.882557192222407 30.00397205486445 21.560337660447757 28-29 21.57442655698561 26.922761379306397 30.10117323943729 21.4016388242707 30-31 21.76359786745 26.726664504868815 29.961945999561284 21.547791628119903 32-33 21.527381276501075 26.872270096153596 29.828837693676235 21.7715109336691 34-35 22.042802679808336 26.881260668577724 29.53720861092911 21.53872804068483 36-37 22.04005746842471 26.719809921681488 29.52315004113924 21.716982568754563 38-39 21.716505302939723 26.852819050441944 29.67947051982675 21.751205126791582 40-41 21.92485456191085 26.62252989037215 29.664455851852345 21.78815969586466 42-43 21.76149704315554 26.811029945015413 29.354434025773386 22.073038986055664 44-45 21.739563139061808 26.897713595777024 29.297338992164494 22.06538427299668 46-47 21.960386899121826 27.052316731052795 28.852105075485234 22.13519129434015 48-49 21.958869671521235 27.13063356518787 28.585165304082594 22.325331459208297 50-51 21.863851900122576 27.303307088117805 28.5273915788544 22.30544943290522 52-53 22.014740290461443 27.309854024529912 28.386922599152992 22.288483085855653 54-55 21.83739454719741 27.24002470908584 28.34131191115422 22.581268832562525 56-57 21.794255621456536 27.507778378613146 28.273469607885716 22.424496392044603 58-59 21.80171741870437 27.11591944026074 28.392615285661083 22.68974785537381 60-61 21.83405561561656 27.036896854941823 28.707960178003173 22.421087351438448 62-63 21.679315529462304 26.94195842581323 28.758716975728504 22.620009068995966 64-65 21.583756621487282 27.017936149142585 28.59644523897517 22.801861990394965 66-67 21.538458967240977 27.122870861788094 28.42373398930802 22.914936181662902 68-69 21.520627264238094 26.916644043685395 28.55960970522315 23.003118986853362 70-71 21.560456546845764 26.853011408418322 28.52338569364621 23.06314635108971 72-73 21.62001816838343 26.83252976236662 28.34518496058941 23.20226710866054 74-75 21.48602905700223 27.056596863875843 28.20126809557198 23.256105983549947 76-77 21.62016688459337 26.882082319484844 28.31536213784902 23.182388658072764 78-79 21.55756239995342 26.88266546523271 28.322715788006725 23.237056346807147 80-81 21.536508569902516 26.856991206115598 28.20811389592205 23.398386328059832 82-83 21.641436050681616 26.79537960491063 28.237373454090225 23.32581089031753 84-85 21.57770772150787 26.56335329364185 28.269224526738284 23.58971445811199 86-87 21.627530374035317 26.45071595727288 28.35629957671465 23.565454091977152 88-89 21.75941396627776 26.24940327292864 28.34265023200749 23.648532528786113 90-91 21.720517749656924 26.28601810443042 28.37087343037797 23.622590715534685 92-93 21.843522695829403 26.14483054495345 28.264209349446467 23.747437409770683 94-95 21.767482972049738 25.980448687414658 28.33077976754553 23.92128857299008 96-97 21.962680195366445 25.712544099753785 28.318679576489554 24.006096128390215 98-99 21.915468718774942 25.635484008831178 28.301001135771354 24.148046136622526 100-101 22.275169303889623 25.183076389876806 28.60588880603641 23.93586550019716 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2804.0 1 1841.5 2 965.5 3 1025.0 4 931.5 5 974.0 6 1381.5 7 2207.5 8 3268.5 9 3791.0 10 3436.5 11 3107.5 12 3221.5 13 3460.0 14 3673.0 15 3851.0 16 4062.0 17 4373.5 18 4721.5 19 5006.5 20 5422.0 21 6136.0 22 6829.0 23 7730.0 24 8974.5 25 10609.0 26 12943.0 27 15836.0 28 18575.0 29 21339.0 30 25256.5 31 29724.5 32 34148.5 33 37966.5 34 41728.0 35 45579.5 36 49672.0 37 53942.5 38 56920.5 39 58416.0 40 59476.5 41 62524.5 42 65647.0 43 68800.5 44 72662.0 45 75528.5 46 77522.5 47 78658.0 48 79685.0 49 79288.5 50 77268.0 51 74647.0 52 72352.0 53 71120.5 54 68514.5 55 63401.0 56 56999.0 57 50569.5 58 45866.5 59 40827.0 60 34260.5 61 28108.0 62 22635.5 63 17555.0 64 13180.0 65 9932.0 66 7557.0 67 5993.0 68 4591.5 69 3255.5 70 2462.5 71 1862.5 72 1457.5 73 1186.0 74 883.0 75 688.0 76 495.5 77 319.5 78 230.0 79 152.5 80 103.0 81 79.5 82 63.5 83 53.5 84 41.5 85 31.0 86 20.5 87 20.0 88 19.0 89 15.0 90 10.0 91 6.5 92 5.5 93 5.0 94 3.5 95 4.0 96 3.5 97 1.0 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26950666206394175 2 0.1661830952564355 3 0.19642413920009585 4 0.16513702140932776 5 0.2379818002170128 6 0.19547316297545245 7 0.24059698483478217 8 0.24749156246344686 9 0.1488277791566933 10-11 0.2066471336150125 12-13 0.21185372844493514 14-15 0.17219801987730504 16-17 0.2851502209593258 18-19 0.23182422916244672 20-21 0.19777928032021272 22-23 0.24461485938390054 24-25 0.23263255895339363 26-27 0.3428031545783324 28-29 0.31377460532109236 30-31 0.28919186991406026 32-33 0.19078960506908366 34-35 0.36037244032861937 36-37 0.3987681053985969 38-39 0.3799387761506574 40-41 0.34201859919300154 42-43 0.37461330929265435 44-45 0.38455101084017795 46-47 0.38937721518024326 48-49 0.4095616855482996 50-51 0.40105044833774106 52-53 0.40107422274335724 54-55 0.3958914023190506 56-57 0.3742566932084131 58-59 0.3490320488497467 60-61 0.31489200238504833 62-63 0.3828630280414359 64-65 0.41766875786338464 66-67 0.4239214265404151 68-69 0.42758268500529223 70-71 0.42748758738282777 72-73 0.4186435084936441 74-75 0.41374598093673065 76-77 0.3975556107121766 78-79 0.3722120943254298 80-81 0.3835524858043024 82-83 0.3927294063721113 84-85 0.3936090593799064 86-87 0.38892550147353766 88-89 0.39665218329876534 90-91 0.4188099293329567 92-93 0.4177400810802329 94-95 0.4265841599694166 96-97 0.4272736177322831 98-99 0.4246346587088976 100-101 0.3969374761661584 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2103102.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.34958103670608 #Duplication Level Percentage of deduplicated Percentage of total 1 79.71135015003694 46.511238851248336 2 11.720899143883953 13.678191088382313 3 3.4378920836121005 6.017986881845237 4 1.5409338497292107 3.5965137814791213 5 0.8281111728045265 2.4159969992479713 6 0.5334622439166923 1.8676379058864063 7 0.3669361710174935 1.4987400292258712 8 0.2760435626479702 1.288562098271103 9 0.22350164859276062 1.173710480076062 >10 1.2359376698628557 13.486994881275985 >50 0.09011430711761462 3.6192328169758627 >100 0.033590081652484075 3.1805645801078537 >500 6.548880668495433E-4 0.265289894737482 >1k 3.274440334247601E-4 0.2850648346764947 >5k 2.4558302506857006E-4 1.1142748765638855 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9948 0.473015574137631 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8265 0.3929909248338882 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5115 0.2432121694525515 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 4.754881123217039E-5 5 0.0 0.0 0.0 0.0 4.754881123217039E-5 6 0.0 0.0 0.0 0.0 4.754881123217039E-5 7 0.0 0.0 0.0 0.0 4.754881123217039E-5 8 0.0 0.0 0.0 0.0 4.754881123217039E-5 9 0.0 0.0 0.0 0.0 4.754881123217039E-5 10-11 0.0 0.0 0.0 4.754881123217039E-5 4.754881123217039E-5 12-13 0.0 0.0 0.0 4.754881123217039E-5 4.754881123217039E-5 14-15 0.0 0.0 0.0 9.509762246434077E-5 4.754881123217039E-5 16-17 0.0 4.754881123217039E-5 0.0 1.1887202808042596E-4 9.509762246434077E-5 18-19 0.0 4.754881123217039E-5 0.0 2.852928673930223E-4 9.509762246434077E-5 20-21 0.0 4.754881123217039E-5 0.0 4.99262517937789E-4 9.509762246434077E-5 22-23 0.0 4.754881123217039E-5 0.0 0.0012124946864203448 9.509762246434077E-5 24-25 0.0 4.754881123217039E-5 0.0 0.0023298917503763485 9.509762246434077E-5 26-27 0.0 4.754881123217039E-5 0.0 0.004231844199663164 1.1887202808042596E-4 28-29 0.0 4.754881123217039E-5 0.0 0.01319479511692728 1.4264643369651115E-4 30-31 0.0 4.754881123217039E-5 0.0 0.03832434185312933 1.4264643369651115E-4 32-33 0.0 4.754881123217039E-5 0.0 0.09091332707590978 1.4264643369651115E-4 34-35 0.0 4.754881123217039E-5 0.0 0.1778801028195494 1.4264643369651115E-4 36-37 0.0 4.754881123217039E-5 0.0 0.3024579882478358 1.4264643369651115E-4 38-39 0.0 4.754881123217039E-5 0.0 0.4786263338630271 1.4264643369651115E-4 40-41 0.0 4.754881123217039E-5 0.0 0.6653742899773762 1.6642083931259633E-4 42-43 0.0 4.754881123217039E-5 0.0 0.864270967361545 1.9019524492868155E-4 44-45 0.0 4.754881123217039E-5 0.0 1.1081963689825791 1.9019524492868155E-4 46-47 0.0 9.509762246434077E-5 0.0 1.3947255054676377 2.3774405616085192E-4 48-49 0.0 9.509762246434077E-5 0.0 1.6867940784612443 2.3774405616085192E-4 50-51 0.0 9.509762246434077E-5 0.0 1.9946013079726996 2.3774405616085192E-4 52-53 0.0 9.509762246434077E-5 0.0 2.307306065041068 2.852928673930223E-4 54-55 0.0 9.509762246434077E-5 0.0 2.6559814978065734 3.566160842412779E-4 56-57 0.0 9.509762246434077E-5 0.0 3.0616204064282186 3.803904898573631E-4 58-59 0.0 9.509762246434077E-5 0.0 3.4946474303195947 3.803904898573631E-4 60-61 0.0 9.509762246434077E-5 0.0 3.963573806691259 3.803904898573631E-4 62-63 0.0 9.509762246434077E-5 0.0 4.431858274111288 3.803904898573631E-4 64-65 0.0 9.509762246434077E-5 0.0 4.9352813130318935 3.803904898573631E-4 66-67 0.0 9.509762246434077E-5 0.0 5.501492557184578 3.803904898573631E-4 68-69 0.0 9.509762246434077E-5 0.0 6.126379034397761 3.803904898573631E-4 70-71 0.0 9.509762246434077E-5 0.0 6.788496230805734 3.803904898573631E-4 72-73 0.0 9.509762246434077E-5 0.0 7.459742798970282 3.803904898573631E-4 74-75 0.0 9.509762246434077E-5 0.0 8.158686549677572 3.803904898573631E-4 76-77 0.0 9.509762246434077E-5 0.0 8.90256392699926 3.803904898573631E-4 78-79 0.0 1.1887202808042596E-4 0.0 9.70949102801481 3.803904898573631E-4 80-81 0.0 1.4264643369651115E-4 0.0 10.578635748527653 3.803904898573631E-4 82-83 0.0 1.4264643369651115E-4 0.0 11.453961814500676 4.2793930108953347E-4 84-85 0.0 1.4264643369651115E-4 0.0 12.349424802030525 4.2793930108953347E-4 86-87 0.0 1.4264643369651115E-4 0.0 13.284591046939235 4.517137067056187E-4 88-89 0.0 1.4264643369651115E-4 0.0 14.2604828486683 4.7548811232170384E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4840 0.0 55.389553 1 GTATCAA 8720 0.0 42.626965 1 ATCAACG 11360 0.0 32.82065 3 TCAACGC 11505 0.0 32.407 4 TATCAAC 11535 0.0 32.322716 2 CAACGCA 11680 0.0 31.840227 5 AACGCAG 12000 0.0 30.989676 6 ACGCAGA 13680 0.0 26.872505 7 CGCAGAG 13645 0.0 26.524279 8 GTGGTAT 2040 0.0 24.931461 1 CGTATAC 100 0.0016672452 23.71772 3 GCAGAGT 14705 0.0 23.707396 9 GAGTACT 9055 0.0 22.408062 12-13 GTACATG 8380 0.0 21.951342 1 TGGTATC 2335 0.0 21.127562 2 TACATGG 8625 0.0 21.009089 2 CAGAGTA 14220 0.0 20.89223 10-11 AGTACTT 9310 0.0 20.774689 12-13 AGAGTAC 13405 0.0 20.409977 10-11 GTACTTT 9985 0.0 20.202145 14-15 >>END_MODULE