FastQCFastQC Report
Thu 26 May 2016
SRR938796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938796_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103102
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94180.44781470418458075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86000.4089197765966653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52840.2512479185507883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.056.7005461
GTATCAA87850.042.3453181
ATCAACG117350.031.5433243
TATCAAC118800.031.4778962
TCAACGC118250.031.3032464
CAACGCA121850.030.417355
AACGCAG125900.029.352326
ACGCAGA142200.025.85437
CGCAGAG144100.025.282968
GTGGTAT20800.025.157631
GTACATG83450.023.714141
GCAGAGT153600.023.2559689
TACATGG86300.022.3260122
TGGTATC22150.021.6393182
GAGTACT93000.021.57839212-13
ACATGGG88600.020.889443
AGAGTAC140450.020.55419710-11
AGTACTT95550.020.53082812-13
CAGAGTA148400.020.38017710-11
GTACTTT100900.019.9594314-15
Produced by FastQC (version 0.11.5)