##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938796_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2103102 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.033693087639115 33.0 31.0 34.0 30.0 34.0 2 32.232450922494486 34.0 31.0 34.0 30.0 34.0 3 32.30255213489408 34.0 31.0 34.0 30.0 34.0 4 35.94562936082035 37.0 35.0 37.0 35.0 37.0 5 35.84546683898356 37.0 35.0 37.0 35.0 37.0 6 35.86787706920539 37.0 35.0 37.0 35.0 37.0 7 35.78361819826142 37.0 35.0 37.0 33.0 37.0 8 35.77265629531996 37.0 35.0 37.0 33.0 37.0 9 37.41291054832338 39.0 37.0 39.0 34.0 39.0 10-11 37.34502368406287 39.0 37.0 39.0 34.0 39.0 12-13 37.272521969928235 39.0 37.0 39.0 33.0 39.0 14-15 38.486600982738835 40.0 38.0 41.0 33.5 41.0 16-17 38.41898633542263 40.0 38.0 41.0 33.0 41.0 18-19 38.54689667928612 40.0 38.0 41.0 34.0 41.0 20-21 38.54817621779638 40.0 38.0 41.0 34.0 41.0 22-23 38.463796572871885 40.0 38.0 41.0 34.0 41.0 24-25 38.38218759717789 40.0 38.0 41.0 34.0 41.0 26-27 38.20419551690788 40.0 38.0 41.0 33.0 41.0 28-29 38.021094792359094 40.0 37.0 41.0 33.0 41.0 30-31 37.77274925324592 40.0 37.0 41.0 33.0 41.0 32-33 37.58409744272983 40.0 37.0 41.0 32.0 41.0 34-35 37.52997762352943 40.0 36.5 41.0 32.0 41.0 36-37 37.39889720042109 40.0 36.0 41.0 32.0 41.0 38-39 37.258249481004725 40.0 36.0 41.0 31.0 41.0 40-41 37.065985149555274 40.0 36.0 41.0 31.0 41.0 42-43 36.83919277334147 40.0 36.0 41.0 31.0 41.0 44-45 36.55500113641659 39.5 35.0 41.0 30.0 41.0 46-47 36.187531798267514 39.0 35.0 41.0 29.5 41.0 48-49 35.83705497878847 39.0 35.0 41.0 28.5 41.0 50-51 35.57441888220353 39.0 35.0 41.0 27.5 41.0 52-53 35.34383068438906 39.0 35.0 40.0 27.0 41.0 54-55 35.06019703276398 38.0 34.5 40.0 26.0 41.0 56-57 34.78695469834558 38.0 34.0 40.0 26.0 41.0 58-59 34.471906498115644 38.0 34.0 40.0 25.5 41.0 60-61 34.11515228457773 37.5 33.5 40.0 24.5 41.0 62-63 33.745146455093476 37.0 33.0 40.0 23.0 41.0 64-65 33.34100509628159 36.0 33.0 39.0 22.5 41.0 66-67 32.938897400126095 36.0 32.0 39.0 22.0 40.0 68-69 32.61266025138106 35.5 32.0 39.0 20.5 40.0 70-71 32.20149640863829 35.0 32.0 38.0 20.0 40.0 72-73 31.72520115524592 35.0 31.0 37.5 18.0 39.5 74-75 31.233335568127462 35.0 31.0 37.0 15.0 39.0 76-77 29.27760850400979 32.5 28.5 35.0 9.5 37.0 78-79 30.2174961081298 34.0 30.0 36.0 8.0 38.0 80-81 30.267703135653903 34.0 30.0 35.5 7.0 37.0 82-83 29.953578571082144 34.0 30.0 35.0 4.0 37.0 84-85 29.564349232704835 34.0 30.0 35.0 2.0 36.0 86-87 28.990313118431725 34.0 29.0 35.0 2.0 36.0 88-89 28.40118239628891 34.0 29.0 35.0 2.0 35.5 90-91 27.88770563672138 33.5 27.5 35.0 2.0 35.0 92-93 27.593726552492463 33.0 27.0 35.0 2.0 35.0 94-95 27.258810556977267 33.0 26.5 35.0 2.0 35.0 96-97 26.660820302581616 33.0 25.5 35.0 2.0 35.0 98-99 25.85937534175708 33.0 24.5 35.0 2.0 35.0 100-101 22.434569745071805 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02986042490669405 1101 2 0.09440768244866149 1101 3 0.03737173771782665 1101 4 0.05572833714973768 1101 5 -0.04747784096652197 1101 6 0.08004490392580266 1101 7 0.1328861146141449 1101 8 0.10771626832532633 1101 9 0.15585632925609616 1101 10-11 0.2437246817460874 1101 12-13 0.11298157401846254 1101 14-15 0.21095560421359494 1101 16-17 0.2632369785096458 1101 18-19 0.22497828330425307 1101 20-21 0.1242910902907397 1101 22-23 0.12238858757923055 1101 24-25 0.11822996394402452 1101 26-27 0.09697314376760602 1101 28-29 0.09549611731210206 1101 30-31 0.17687247610965784 1101 32-33 0.23031309786234289 1101 34-35 0.39629272431478313 1101 36-37 0.40860808880933774 1101 38-39 0.28201490258486217 1101 40-41 0.33554422078071156 1101 42-43 0.23091295572788795 1101 44-45 0.3430065491425438 1101 46-47 0.5165001417161008 1101 48-49 0.4108129209609288 1101 50-51 0.5583629975041404 1101 52-53 0.46731231249124505 1101 54-55 0.5744495659248514 1101 56-57 0.5737935957098728 1101 58-59 0.5352876914110709 1101 60-61 0.5474943214213965 1101 62-63 0.4479344325793164 1101 64-65 0.43331352416306146 1101 66-67 0.5045834229604367 1101 68-69 0.3587038832380216 1101 70-71 0.3808895227784248 1101 72-73 0.11508926694604682 1101 74-75 -0.016376578545486353 1101 76-77 0.06552106704648608 1101 78-79 -0.00834892210120941 1101 80-81 0.20112422639737204 1101 82-83 0.18114336419572297 1101 84-85 0.13716876473496598 1101 86-87 0.10776366886585009 1101 88-89 0.19125947084270223 1101 90-91 0.13862558788515855 1101 92-93 -0.05876066051585482 1101 94-95 0.21716964519643867 1101 96-97 0.19473932996353227 1101 98-99 0.1556173182420224 1101 100-101 0.6939605637833814 1102 1 0.2315491536348233 1102 2 0.1459713390698738 1102 3 0.14147923270321172 1102 4 0.10104508680119295 1102 5 -0.04974477245680475 1102 6 0.04577419036480279 1102 7 0.08185077767329574 1102 8 0.185431380357862 1102 9 0.2333845995217274 1102 10-11 0.23056106465664783 1102 12-13 0.20779823922234897 1102 14-15 0.29383455557892546 1102 16-17 0.321419258878997 1102 18-19 0.2984294695405083 1102 20-21 0.26671897121255483 1102 22-23 0.18097159436970145 1102 24-25 0.22933837076640629 1102 26-27 0.1831772639727447 1102 28-29 0.3083162651481999 1102 30-31 0.1776778624627866 1102 32-33 0.2432994611176511 1102 34-35 0.2782539630068612 1102 36-37 0.20884940970196197 1102 38-39 0.28038040589110125 1102 40-41 0.2656211479613475 1102 42-43 0.27973839708624837 1102 44-45 0.11000749048086789 1102 46-47 0.1318671886795144 1102 48-49 0.007348899186986557 1102 50-51 0.15993141121508359 1102 52-53 -0.12522094964554498 1102 54-55 -0.03917075636243794 1102 56-57 -0.05138743798237044 1102 58-59 -0.06603389449200847 1102 60-61 0.06160542294457372 1102 62-63 -0.11140872518912914 1102 64-65 -0.06685217498284857 1102 66-67 0.13417674956254189 1102 68-69 -0.05405797488368336 1102 70-71 -0.04798559390548007 1102 72-73 -0.0013159810458382992 1102 74-75 0.03973458542217401 1102 76-77 -0.04367171997627395 1102 78-79 -0.38810542794382386 1102 80-81 0.027114432446968095 1102 82-83 -0.08292428432442378 1102 84-85 0.13648236708602823 1102 86-87 0.24014339623912662 1102 88-89 -0.058480118444673934 1102 90-91 -0.1419045412102129 1102 92-93 0.11881240260494863 1102 94-95 0.1225810007014978 1102 96-97 0.09142849385399643 1102 98-99 0.41956007779166526 1102 100-101 1.1021200912507183 1103 1 0.1857860195965344 1103 2 0.1459054480348243 1103 3 0.09899544479414146 1103 4 0.019092568894762962 1103 5 -0.13999376816158104 1103 6 -0.014919417770350663 1103 7 0.1051037963515924 1103 8 0.13341004614404994 1103 9 0.2905801901805489 1103 10-11 0.08453458370133404 1103 12-13 0.1722554563905092 1103 14-15 0.12542612094717498 1103 16-17 0.20149612692603824 1103 18-19 -0.014484159621851234 1103 20-21 0.010250788617632622 1103 22-23 0.024123416003391185 1103 24-25 0.024928450328026486 1103 26-27 0.11422446802063035 1103 28-29 0.1338940064324774 1103 30-31 0.2119665433966702 1103 32-33 0.17593167039336777 1103 34-35 0.21533888568988857 1103 36-37 0.24234957044712502 1103 38-39 0.25995780541089175 1103 40-41 0.24542554571728914 1103 42-43 0.2401657619951436 1103 44-45 0.20189905555948684 1103 46-47 0.23819649324670422 1103 48-49 0.13477600632435127 1103 50-51 0.18714332257643207 1103 52-53 0.07417980397411839 1103 54-55 0.048105177914507635 1103 56-57 -0.042601483923618844 1103 58-59 0.07670780975426794 1103 60-61 0.10115814590970018 1103 62-63 0.0900523904112589 1103 64-65 0.02542278903050743 1103 66-67 0.12125377980291319 1103 68-69 0.009282497851906157 1103 70-71 0.00984284032077909 1103 72-73 0.12528603999874477 1103 74-75 0.17507766773238842 1103 76-77 -0.03933775524752292 1103 78-79 -0.2972340877925781 1103 80-81 0.08668716890889172 1103 82-83 0.06841257960986269 1103 84-85 0.11266674033918989 1103 86-87 0.074292040621593 1103 88-89 -0.18645317987249754 1103 90-91 -0.28484536671626515 1103 92-93 -0.2732631505177068 1103 94-95 0.005611742127701547 1103 96-97 0.19868534145310335 1103 98-99 0.32261300494659295 1103 100-101 1.0117251939683243 1104 1 0.13756545532245212 1104 2 0.11526628043913689 1104 3 0.035045812115413355 1104 4 -0.015109757949709035 1104 5 -0.1422834517221645 1104 6 0.046719092363140646 1104 7 0.0762069035750983 1104 8 0.061227843359837664 1104 9 0.14498518689323703 1104 10-11 0.1407325941635591 1104 12-13 0.06946267132347828 1104 14-15 0.02424912068990892 1104 16-17 0.09855326896646233 1104 18-19 0.13551155258122094 1104 20-21 0.1032945527316258 1104 22-23 0.08926076843171415 1104 24-25 0.047926763794563954 1104 26-27 0.019846484428605038 1104 28-29 0.00663076428629239 1104 30-31 -0.03786194472613147 1104 32-33 -0.03263320397417857 1104 34-35 -0.07818797021155177 1104 36-37 -0.022787781981200794 1104 38-39 0.11108120820653511 1104 40-41 0.022153527880050206 1104 42-43 0.054837888456567896 1104 44-45 0.16114711890453748 1104 46-47 0.12571042858672854 1104 48-49 0.3591557050351568 1104 50-51 0.13716118839697344 1104 52-53 0.151062861459188 1104 54-55 0.12247558639862888 1104 56-57 0.13563080032872676 1104 58-59 0.24132633875406384 1104 60-61 0.23243476598231183 1104 62-63 0.2024977210004053 1104 64-65 0.2375243812324328 1104 66-67 0.07194197121016543 1104 68-69 0.11177871029796194 1104 70-71 0.016460340249317085 1104 72-73 0.23523087034491041 1104 74-75 0.3350696638447843 1104 76-77 0.1020187607745413 1104 78-79 -0.11763042315547168 1104 80-81 0.030368385499809136 1104 82-83 0.13990659098681846 1104 84-85 0.256355103258322 1104 86-87 0.4712176902228258 1104 88-89 0.4289850329737419 1104 90-91 0.308815840155642 1104 92-93 0.38105037319381196 1104 94-95 0.3496266922463285 1104 96-97 0.30334617671594444 1104 98-99 0.4004758240301456 1104 100-101 1.2392106869220463 1105 1 0.12242158789798196 1105 2 0.12297796651838411 1105 3 0.09727414422904701 1105 4 0.0665690031102244 1105 5 -0.0585571930959361 1105 6 -0.03421371478999191 1105 7 0.09239218831199736 1105 8 0.0960163795620872 1105 9 0.04809462006402043 1105 10-11 0.043264209431903566 1105 12-13 0.03220088299474355 1105 14-15 0.08400146490478022 1105 16-17 0.061989102567672205 1105 18-19 0.08964393660157555 1105 20-21 -0.03377094393643176 1105 22-23 -0.0064848777007000535 1105 24-25 -0.007525174386096012 1105 26-27 0.05281880981222997 1105 28-29 0.1888213427425356 1105 30-31 0.12474893954386346 1105 32-33 0.13959528772616636 1105 34-35 0.09332317920036814 1105 36-37 0.014266657607251432 1105 38-39 0.06495471977543588 1105 40-41 0.09049790689393689 1105 42-43 0.30150184214905806 1105 44-45 0.362026897671754 1105 46-47 0.2724893987052539 1105 48-49 0.050070775264714484 1105 50-51 0.10345453613870603 1105 52-53 0.24799788458785343 1105 54-55 0.29777543843894705 1105 56-57 0.21414153709500283 1105 58-59 0.2037633874941278 1105 60-61 0.21557169366445095 1105 62-63 0.3298592054304379 1105 64-65 0.34067792873113945 1105 66-67 0.250718875820624 1105 68-69 0.20935116836654544 1105 70-71 0.30688365541256957 1105 72-73 0.40762168453354164 1105 74-75 0.4627435962666482 1105 76-77 0.5010825567041692 1105 78-79 0.28237379449344857 1105 80-81 0.3768760582011339 1105 82-83 0.5747366197352228 1105 84-85 0.5660766700351019 1105 86-87 0.651904847823829 1105 88-89 0.4888608289124896 1105 90-91 0.24680659780497294 1105 92-93 0.4245956604840977 1105 94-95 0.5082420951001936 1105 96-97 0.5981208516720002 1105 98-99 0.7797695616194318 1105 100-101 1.6380139005425427 1106 1 0.13581690964169724 1106 2 0.11311646594189995 1106 3 -0.0033437324781075972 1106 4 0.08991955260769657 1106 5 -0.033695402359377624 1106 6 0.014272361358962371 1106 7 0.09612463225909806 1106 8 0.10734685655238962 1106 9 0.17973641143564834 1106 10-11 0.17945111193483854 1106 12-13 0.1779156567410567 1106 14-15 0.15561702142631617 1106 16-17 0.17624866334197264 1106 18-19 0.08060889949940986 1106 20-21 0.1300803670943651 1106 22-23 0.1909443040807517 1106 24-25 0.16060988472442972 1106 26-27 0.09128746540756083 1106 28-29 0.23227834266260317 1106 30-31 0.20990697823697957 1106 32-33 0.3184007917889602 1106 34-35 0.20857968617680456 1106 36-37 0.26199426628314626 1106 38-39 0.27453996606658393 1106 40-41 0.38168142054625065 1106 42-43 0.268705761171411 1106 44-45 0.2560281393378716 1106 46-47 0.34052262805543876 1106 48-49 0.2915783374930996 1106 50-51 0.4320797577279265 1106 52-53 0.3129355987177789 1106 54-55 0.24774319234816744 1106 56-57 0.2748273660056668 1106 58-59 0.3099976682092205 1106 60-61 0.22807573126919323 1106 62-63 0.3487282600232291 1106 64-65 0.26522930294017755 1106 66-67 0.2686785797040585 1106 68-69 0.22569758226182302 1106 70-71 0.22974312267867703 1106 72-73 0.32640631897017514 1106 74-75 0.6398804061920949 1106 76-77 0.43472272974906545 1106 78-79 0.29925256999605665 1106 80-81 0.3527721874657992 1106 82-83 0.47628530804746916 1106 84-85 0.47793282947641913 1106 86-87 0.6426743632784095 1106 88-89 0.8017917850501384 1106 90-91 0.7100678436975691 1106 92-93 0.7155942381522813 1106 94-95 0.908441155857524 1106 96-97 1.0242547479523694 1106 98-99 1.0821794231200066 1106 100-101 1.7361938851914331 1107 1 0.07955937227742282 1107 2 0.002436810266509326 1107 3 0.024514905705736112 1107 4 0.012326888237822686 1107 5 6.863151393972089E-4 1107 6 -0.004631159298682519 1107 7 0.03951620595818639 1107 8 0.028860019206149445 1107 9 0.013461480776697954 1107 10-11 -0.06101577548596282 1107 12-13 0.025188062079323004 1107 14-15 -0.055198347293178074 1107 16-17 -0.05867579836741044 1107 18-19 -0.07953184475222486 1107 20-21 -0.06739139714282061 1107 22-23 0.038791951343441156 1107 24-25 -0.035355540299242705 1107 26-27 -0.038580308844537115 1107 28-29 -0.0377763636217594 1107 30-31 -0.07254609171731374 1107 32-33 -0.05335264881202306 1107 34-35 -0.04640158715056231 1107 36-37 -0.10161941471454128 1107 38-39 -0.17525149793922878 1107 40-41 -0.15139736535643777 1107 42-43 -0.11806367456679112 1107 44-45 0.0077817742773333975 1107 46-47 -0.2933506783656128 1107 48-49 -0.1840502125582404 1107 50-51 -0.3093780400499142 1107 52-53 -0.4669700175565552 1107 54-55 -0.1606236193885877 1107 56-57 -0.20772989097135053 1107 58-59 -0.06126884651354203 1107 60-61 -0.078539442895881 1107 62-63 -0.27407952085795984 1107 64-65 -0.28286472233893534 1107 66-67 -0.15532146780090272 1107 68-69 -0.1951677854319982 1107 70-71 -0.2965124389109697 1107 72-73 -0.40729964041955924 1107 74-75 -0.2674294201016849 1107 76-77 -0.4109328873350151 1107 78-79 -0.23911924055846612 1107 80-81 -0.13341703991994436 1107 82-83 -0.15167201090193672 1107 84-85 -0.13508277482450737 1107 86-87 -0.08618716946924465 1107 88-89 -0.00572339224594387 1107 90-91 0.11563240240344541 1107 92-93 0.26665473248461424 1107 94-95 0.4472171904670468 1107 96-97 0.5209991464153667 1107 98-99 0.7224922433061636 1107 100-101 1.3339491676894148 1108 1 0.1491421707354874 1108 2 0.11586522681812994 1108 3 0.05783637761697946 1108 4 0.073333200058471 1108 5 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sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 17.0 5 74.0 6 169.0 7 447.0 8 1051.0 9 1872.0 10 3175.0 11 4761.0 12 6696.0 13 9009.0 14 10291.0 15 13461.0 16 17041.0 17 22782.0 18 21744.0 19 14626.0 20 13029.0 21 13848.0 22 14512.0 23 16059.0 24 18563.0 25 22117.0 26 25852.0 27 31233.0 28 37632.0 29 45876.0 30 55275.0 31 66685.0 32 81407.0 33 103027.0 34 134815.0 35 187096.0 36 279452.0 37 433477.0 38 363245.0 39 32673.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.67789441075195 20.338095294832538 15.1488996651932 22.835110629222317 2 16.08947164711935 21.885101150586134 38.670449650088294 23.35497755220622 3 18.23649066949677 25.701654032947523 31.58386992166809 24.477985375887616 4 13.407005461456459 18.25703175594907 36.84852185010523 31.487440932489246 5 13.425588585017836 37.530891795481566 34.389155766944114 14.654363852556479 6 30.28341066634459 36.84956819364098 17.94775718417176 14.919263955842677 7 26.59000847319816 33.99540298093007 21.797991728408796 17.616596817462966 8 25.552208119244813 34.72451645236417 20.885815333730843 18.837460094660173 9 26.1794720370196 16.707320900270172 21.141723035782384 35.97148402692784 10-11 24.212663009212108 26.965453886687378 27.976341613483317 20.845541490617194 12-13 25.1894820127602 24.90604354900523 28.24104584561281 21.663428592621756 14-15 22.52413339914089 26.191525660666958 26.773095170847633 24.51124576934452 16-17 21.398198852760647 29.176669011675195 27.534196191861483 21.890935943702676 18-19 21.313778409225993 27.37278077810777 30.29177852524509 21.021662287421155 20-21 22.256267171064454 26.667584358723445 30.117821199352196 20.9583272708599 22-23 22.04277300863201 26.648469736608117 29.93171515218948 21.377042102570393 24-25 21.82805730218687 26.864798373445627 30.08376697972893 21.22337734463857 26-27 21.57097647950233 27.026497559175695 30.039815404117913 21.36271055720406 28-29 21.50737339415777 27.052278015997324 30.078545881274422 21.361802708570483 30-31 21.777688386012663 26.87696554898431 29.90998534545638 21.43536071954665 32-33 21.682757184387633 26.94771818009778 29.736551056487038 21.63297357902755 34-35 21.98636497862306 26.951041076726355 29.62046004525987 21.44213389939072 36-37 21.98838146331929 26.890913159154362 29.495275377295705 21.62543000023065 38-39 21.696881936345232 26.88006981076669 29.71743997070611 21.705608282181966 40-41 21.893925146206062 26.661204471169995 29.728030692276214 21.716839690347726 42-43 21.84635049312905 26.817917794943625 29.34444186341225 21.991289848515073 44-45 21.702940467410638 27.01039403566183 29.24975499671189 22.03691050021564 46-47 21.981666530755763 27.03184618277789 28.80076420758278 22.18572307888356 48-49 22.033743567431856 27.134554644584636 28.514895503829525 22.31680628415398 50-51 21.85229766608982 27.41657975138147 28.42898928083119 22.302133301697516 52-53 22.027581163443333 27.38240941238228 28.296416436292677 22.29359298788171 54-55 21.778948429510315 27.34330051514382 28.179398811850305 22.69835224349556 56-57 21.782243186184072 27.625221104285142 28.01769309774236 22.574842611788423 58-59 21.80139485067823 27.327162262240012 28.18713394679502 22.684308940286744 60-61 21.753059410321292 27.350758573041528 28.36204664582878 22.5341353708084 62-63 21.51885883859691 27.343800335269297 28.408545444300763 22.728795381833024 64-65 21.455708601512455 27.31380570898191 28.249532113023236 22.980953576482403 66-67 21.419297194059205 27.461139157231067 28.048304683177644 23.071258965532078 68-69 21.352095812938817 27.26273335812216 28.190246008507447 23.194924820431574 70-71 21.432470432946396 27.187923927571717 28.104380151024603 23.27522548845728 72-73 21.435194298707337 27.215251566495585 27.805070795425042 23.544483339372032 74-75 21.342685918651622 27.426114092569957 27.60390006992091 23.62729991885751 76-77 21.359891678338712 27.244410739297244 27.813851166189636 23.58184641617441 78-79 21.270497589177115 27.26216559102359 27.789801670446835 23.67753514935246 80-81 21.205713602364906 27.281497931730343 27.66305617957226 23.849732286332493 82-83 21.23690585242214 27.235993384009642 27.632125449904226 23.894975313663988 84-85 21.14265023760141 26.97791167523021 27.58099702249344 24.298441064674943 86-87 21.118240661475035 26.977426845553566 27.607733995341476 24.296598497629923 88-89 21.184146298985144 26.772946177597372 27.567072230972684 24.475835292444803 90-91 21.03087404912554 26.862410825499722 27.56964924955113 24.537065875823604 92-93 20.99861085454437 26.769900406140202 27.49851825277217 24.732970486543262 94-95 20.87912897812888 26.576451921051518 27.5641162801645 24.9803028206551 96-97 21.00579716209135 26.486283568335857 27.512697934190417 24.99522133538237 98-99 20.802937755753167 26.480574884147323 27.391039521620918 25.32544783847859 100-101 20.82172735062161 26.139494323093444 27.843612374600706 25.195165951684245 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1292.0 1 885.5 2 566.5 3 662.0 4 633.0 5 658.0 6 982.0 7 1746.5 8 2641.0 9 3160.0 10 2920.0 11 2581.0 12 2747.0 13 2949.0 14 3148.0 15 3430.0 16 3649.5 17 3966.5 18 4320.0 19 4668.0 20 5145.0 21 5851.0 22 6721.0 23 7683.5 24 8868.5 25 10498.5 26 12821.5 27 15798.5 28 18546.0 29 21503.5 30 25562.0 31 30039.5 32 34386.0 33 38053.0 34 41805.5 35 45296.0 36 49611.0 37 54152.5 38 56874.5 39 58213.5 40 59360.0 41 62788.0 42 66149.0 43 69600.5 44 73755.5 45 76727.5 46 78618.5 47 79664.0 48 80955.5 49 80764.5 50 78697.0 51 75910.0 52 73243.0 53 71426.5 54 68749.5 55 63934.0 56 57755.5 57 51339.0 58 46537.5 59 41138.5 60 33967.5 61 27661.5 62 22448.0 63 17398.5 64 12725.5 65 9432.0 66 7066.0 67 5512.0 68 4127.0 69 2908.0 70 2206.5 71 1602.5 72 1205.5 73 974.0 74 728.0 75 577.0 76 402.0 77 230.0 78 146.0 79 87.0 80 60.5 81 42.0 82 27.5 83 18.5 84 10.0 85 8.5 86 8.5 87 6.0 88 3.5 89 2.0 90 2.5 91 1.5 92 0.5 93 0.5 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23189555237929496 2 0.0 3 0.0 4 0.0 5 0.028624384361766574 6 0.003613709653644949 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002543861400921116 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.852928673930223E-4 26-27 0.0010222994414916633 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0064190895163430016 36-37 0.016237919035786186 38-39 0.01305214868323077 40-41 0.007726681825227687 42-43 0.0072987425241381534 44-45 0.0032808679750197566 46-47 4.0416489547344826E-4 48-49 0.0016404339875098783 50-51 2.3774405616085194E-5 52-53 0.0 54-55 0.0 56-57 0.0010460738471077483 58-59 4.5171370670561863E-4 60-61 0.0019732756661350707 62-63 7.845553853308115E-4 64-65 0.0014264643369651115 66-67 0.00558698531978002 68-69 1.9019524492868155E-4 70-71 4.754881123217039E-5 72-73 0.0 74-75 5.468113291699594E-4 76-77 0.057724256835854845 78-79 0.13720209481042764 80-81 7.370065740986409E-4 82-83 7.370065740986409E-4 84-85 0.0 86-87 0.1056296841522665 88-89 0.3489607256328985 90-91 0.38621521923330393 92-93 4.99262517937789E-4 94-95 4.2793930108953347E-4 96-97 1.9019524492868155E-4 98-99 0.0 100-101 9.509762246434078E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2103102.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.567735682068175 #Duplication Level Percentage of deduplicated Percentage of total 1 78.67729650130467 44.5059651266551 2 12.146912390039075 13.742466588659388 3 3.6690491345569907 6.2264940494442245 4 1.6094904181649865 3.641809142303133 5 0.9204665519706439 2.603435430803002 6 0.5750103901241581 1.9516221457791767 7 0.40417834113090606 1.6004417498656918 8 0.2826598610710574 1.2791542647198149 9 0.22009463931187526 1.120522984347086 >10 1.3574999750014098 14.21466077604952 >50 0.09772713501779201 3.76577852724261 >100 0.038101793690302116 3.5331249908541182 >500 7.564343080324465E-4 0.2819846238243649 >1k 5.042895386882909E-4 0.4246647546786715 >5k 2.5214476934414546E-4 1.1078748447741158 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9418 0.44781470418458075 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8600 0.4089197765966653 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5284 0.2512479185507883 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 4.754881123217039E-5 0.0 0.0 0.0 5 0.0 4.754881123217039E-5 0.0 0.0 0.0 6 0.0 4.754881123217039E-5 0.0 0.0 0.0 7 0.0 4.754881123217039E-5 0.0 0.0 0.0 8 0.0 4.754881123217039E-5 0.0 0.0 0.0 9 0.0 4.754881123217039E-5 0.0 0.0 0.0 10-11 0.0 4.754881123217039E-5 0.0 4.754881123217039E-5 0.0 12-13 0.0 4.754881123217039E-5 0.0 4.754881123217039E-5 0.0 14-15 0.0 9.509762246434077E-5 0.0 9.509762246434077E-5 7.132321684825559E-5 16-17 0.0 9.509762246434077E-5 0.0 1.1887202808042596E-4 9.509762246434077E-5 18-19 0.0 9.509762246434077E-5 0.0 2.852928673930223E-4 9.509762246434077E-5 20-21 0.0 9.509762246434077E-5 0.0 4.99262517937789E-4 9.509762246434077E-5 22-23 0.0 9.509762246434077E-5 0.0 0.0011411714695720894 9.509762246434077E-5 24-25 4.754881123217039E-5 9.509762246434077E-5 0.0 0.002353666155992434 9.509762246434077E-5 26-27 4.754881123217039E-5 1.1887202808042596E-4 0.0 0.004445813850207931 9.509762246434077E-5 28-29 4.754881123217039E-5 1.4264643369651115E-4 0.0 0.013884252879793753 9.509762246434077E-5 30-31 4.754881123217039E-5 1.4264643369651115E-4 0.0 0.0398221294069427 9.509762246434077E-5 32-33 4.754881123217039E-5 1.4264643369651115E-4 0.0 0.09374248134422392 9.509762246434077E-5 34-35 4.754881123217039E-5 1.4264643369651115E-4 0.0 0.18256366072591818 9.509762246434077E-5 36-37 4.754881123217039E-5 1.4264643369651115E-4 0.0 0.30944766349896485 9.509762246434077E-5 38-39 4.754881123217039E-5 1.4264643369651115E-4 0.0 0.4888255538723276 1.4264643369651115E-4 40-41 4.754881123217039E-5 1.4264643369651115E-4 0.0 0.6783551154437588 1.4264643369651115E-4 42-43 4.754881123217039E-5 1.4264643369651115E-4 0.0 0.8798194286344647 1.6642083931259633E-4 44-45 4.754881123217039E-5 1.6642083931259633E-4 0.0 1.126883051796822 2.1396965054476673E-4 46-47 4.754881123217039E-5 1.9019524492868155E-4 0.0 1.4167168306625166 2.3774405616085192E-4 48-49 4.754881123217039E-5 1.9019524492868155E-4 0.0 1.7125417597434647 2.3774405616085192E-4 50-51 4.754881123217039E-5 1.9019524492868155E-4 0.0 2.0240577965310287 2.3774405616085192E-4 52-53 4.754881123217039E-5 1.9019524492868155E-4 0.0 2.3405426840923553 2.3774405616085192E-4 54-55 4.754881123217039E-5 1.9019524492868155E-4 0.0 2.6940205467923093 2.615184617769371E-4 56-57 4.754881123217039E-5 1.9019524492868155E-4 0.0 3.10436678772594 2.852928673930223E-4 58-59 4.754881123217039E-5 1.9019524492868155E-4 0.0 3.542196241551765 2.852928673930223E-4 60-61 4.754881123217039E-5 1.9019524492868155E-4 0.0 4.017779451495933 2.852928673930223E-4 62-63 4.754881123217039E-5 1.9019524492868155E-4 0.0 4.492910947733396 2.852928673930223E-4 64-65 4.754881123217039E-5 1.9019524492868155E-4 0.0 5.003062143443351 2.852928673930223E-4 66-67 4.754881123217039E-5 1.9019524492868155E-4 0.0 5.575502281867451 2.852928673930223E-4 68-69 4.754881123217039E-5 1.9019524492868155E-4 0.0 6.2065463301352 2.852928673930223E-4 70-71 4.754881123217039E-5 1.9019524492868155E-4 0.0 6.876485305990865 3.3284167862519267E-4 72-73 7.132321684825559E-5 1.9019524492868155E-4 0.0 7.556314434582822 4.2793930108953347E-4 74-75 9.509762246434077E-5 1.9019524492868155E-4 0.0 8.265314758865713 4.2793930108953347E-4 76-77 9.509762246434077E-5 1.9019524492868155E-4 0.0 9.018297733538365 4.2793930108953347E-4 78-79 9.509762246434077E-5 1.9019524492868155E-4 0.0 9.834449303932953 4.2793930108953347E-4 80-81 9.509762246434077E-5 1.9019524492868155E-4 0.0 10.713151335503461 4.517137067056187E-4 82-83 9.509762246434077E-5 2.1396965054476673E-4 0.0 11.597416577988135 4.7548811232170384E-4 84-85 9.509762246434077E-5 2.3774405616085192E-4 0.0 12.50376824329015 4.7548811232170384E-4 86-87 9.509762246434077E-5 2.3774405616085192E-4 0.0 13.446542297996007 4.7548811232170384E-4 88-89 9.509762246434077E-5 2.3774405616085192E-4 0.0 14.431611020292882 5.230369235538743E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4950 0.0 56.700546 1 GTATCAA 8785 0.0 42.345318 1 ATCAACG 11735 0.0 31.543324 3 TATCAAC 11880 0.0 31.477896 2 TCAACGC 11825 0.0 31.303246 4 CAACGCA 12185 0.0 30.41735 5 AACGCAG 12590 0.0 29.35232 6 ACGCAGA 14220 0.0 25.8543 7 CGCAGAG 14410 0.0 25.28296 8 GTGGTAT 2080 0.0 25.15763 1 GTACATG 8345 0.0 23.71414 1 GCAGAGT 15360 0.0 23.255968 9 TACATGG 8630 0.0 22.326012 2 TGGTATC 2215 0.0 21.639318 2 GAGTACT 9300 0.0 21.578392 12-13 ACATGGG 8860 0.0 20.88944 3 AGAGTAC 14045 0.0 20.554197 10-11 AGTACTT 9555 0.0 20.530828 12-13 CAGAGTA 14840 0.0 20.380177 10-11 GTACTTT 10090 0.0 19.95943 14-15 >>END_MODULE