##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938795_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1376631 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52877132652105 33.0 31.0 34.0 30.0 34.0 2 31.56211068906628 33.0 31.0 34.0 30.0 34.0 3 31.551741171018232 33.0 31.0 34.0 28.0 34.0 4 35.1225767834663 37.0 35.0 37.0 32.0 37.0 5 35.176459777529345 37.0 35.0 37.0 33.0 37.0 6 35.2900951671145 37.0 35.0 37.0 33.0 37.0 7 35.21620172726025 37.0 35.0 37.0 32.0 37.0 8 35.19574163301567 37.0 35.0 37.0 32.0 37.0 9 36.72037241642822 39.0 37.0 39.0 32.0 39.0 10-11 36.62446472584156 39.0 37.0 39.0 32.0 39.0 12-13 36.511463129916436 39.0 37.0 39.0 32.0 39.0 14-15 37.57729667572501 40.0 37.0 41.0 32.0 41.0 16-17 37.53337967835971 40.0 37.0 41.0 32.0 41.0 18-19 37.53185348869813 40.0 37.0 41.0 32.0 41.0 20-21 37.44537134497189 40.0 37.0 41.0 32.0 41.0 22-23 37.333640242011114 40.0 37.0 41.0 32.0 41.0 24-25 37.289056036076474 40.0 37.0 41.0 31.5 41.0 26-27 37.16757468050625 40.0 36.5 41.0 31.0 41.0 28-29 37.040310729600016 40.0 36.0 41.0 31.0 41.0 30-31 36.772712876580584 39.0 36.0 41.0 30.5 41.0 32-33 36.46891323818801 39.0 35.0 41.0 30.0 41.0 34-35 36.078641625824204 39.0 35.0 41.0 29.5 41.0 36-37 35.769619455031886 39.0 35.0 41.0 28.5 41.0 38-39 35.5597411361505 38.0 35.0 40.0 27.0 41.0 40-41 35.3537447580361 38.0 35.0 40.0 26.5 41.0 42-43 35.01444795300992 38.0 35.0 40.0 25.5 41.0 44-45 34.78596552017207 38.0 35.0 40.5 24.0 41.0 46-47 34.422956841739 38.0 34.0 41.0 22.0 41.0 48-49 34.05957406160402 38.0 34.0 40.0 18.5 41.0 50-51 32.869388020464456 37.0 32.5 39.5 14.0 40.5 52-53 32.97406095024738 37.5 33.0 39.5 13.0 40.5 54-55 33.480295010064424 38.0 33.0 40.0 11.0 41.0 56-57 33.36100959516385 38.0 33.0 40.0 8.5 41.0 58-59 33.13182254358648 38.0 33.0 40.0 7.5 41.0 60-61 32.82300594712744 37.0 33.0 40.0 3.5 41.0 62-63 32.464746907486465 37.0 32.0 40.0 2.0 41.0 64-65 32.05997031884361 36.0 32.0 39.5 2.0 41.0 66-67 31.620921292633973 36.0 31.0 39.0 2.0 41.0 68-69 31.18582612188742 35.0 31.0 39.0 2.0 40.5 70-71 30.726775003613895 35.0 30.5 38.5 2.0 40.0 72-73 30.277185389548833 35.0 30.0 37.0 2.0 40.0 74-75 29.81069473228483 35.0 29.5 37.0 2.0 39.0 76-77 29.34407767949436 35.0 29.0 36.0 2.0 39.0 78-79 28.883425188013348 34.0 29.0 36.0 2.0 38.5 80-81 28.396061108604993 34.0 28.0 35.5 2.0 37.0 82-83 27.887217417012984 34.0 26.5 35.0 2.0 37.0 84-85 27.42855202301851 34.0 26.0 35.0 2.0 36.0 86-87 27.027595266996023 34.0 25.5 35.0 2.0 36.0 88-89 26.595650541067286 34.0 24.5 35.0 2.0 36.0 90-91 26.12024427751518 33.0 23.5 35.0 2.0 35.0 92-93 25.61638231305266 33.0 19.5 35.0 2.0 35.0 94-95 25.06092845504714 33.0 14.5 35.0 2.0 35.0 96-97 24.45165915920824 32.5 2.0 35.0 2.0 35.0 98-99 23.757431730071456 32.0 2.0 35.0 2.0 35.0 100-101 21.713085423762795 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.413990451282622 1101 2 0.33288173420525524 1101 3 0.3403160520743924 1101 4 0.39216325255618756 1101 5 0.29668275826889356 1101 6 0.2667752531599348 1101 7 0.4633527474708288 1101 8 0.35832757376941515 1101 9 0.4559889370979704 1101 10-11 0.4132761009141248 1101 12-13 0.36925136516622814 1101 14-15 0.4576990539356274 1101 16-17 0.4888867159054655 1101 18-19 0.32820174076707787 1101 20-21 0.5233706169846286 1101 22-23 0.4982702751301389 1101 24-25 0.3711894078051188 1101 26-27 0.49868855809751267 1101 28-29 0.22774945133176772 1101 30-31 0.25303936155772533 1101 32-33 0.2933734766231595 1101 34-35 0.3329680986741721 1101 36-37 0.39957182589536444 1101 38-39 0.3128042762342389 1101 40-41 0.12988130219824967 1101 42-43 0.25853553223198844 1101 44-45 0.34620615787059705 1101 46-47 0.3693207566097385 1101 48-49 0.3173831016681774 1101 50-51 0.030411735762498893 1101 52-53 0.08123471702826635 1101 54-55 0.16606337152717288 1101 56-57 0.1612045812274019 1101 58-59 0.10904256583250316 1101 60-61 -0.08545944182593246 1101 62-63 -0.19231714329124117 1101 64-65 -0.2134624548239259 1101 66-67 -0.1835799654450021 1101 68-69 -0.27457952337931957 1101 70-71 -0.4691445396013414 1101 72-73 -0.3134443354361558 1101 74-75 -0.44074571325203493 1101 76-77 -0.41858279190958925 1101 78-79 -0.5143270866707184 1101 80-81 -0.6001217517493451 1101 82-83 -0.6504144055518033 1101 84-85 -0.7175877218315883 1101 86-87 -0.7566835437848844 1101 88-89 -0.7396483055412908 1101 90-91 -0.8180702029157452 1101 92-93 -0.8260389218891859 1101 94-95 -1.0132039187117918 1101 96-97 -1.1720339508853925 1101 98-99 -1.2133881367823776 1101 100-101 -0.9594993905410725 1102 1 0.30254670892332314 1102 2 0.3083817857569606 1102 3 0.3010502220182758 1102 4 0.20333487555119234 1102 5 0.1441173244244638 1102 6 0.13931365918117677 1102 7 0.30785623089327174 1102 8 0.2387552319963291 1102 9 0.32648920958556005 1102 10-11 0.3639392767938219 1102 12-13 0.3971482028372719 1102 14-15 0.28535617617208686 1102 16-17 0.3599328841407754 1102 18-19 0.41356952536931857 1102 20-21 0.5544912774356376 1102 22-23 0.48783473685666934 1102 24-25 0.47406820237893754 1102 26-27 0.3361303783146212 1102 28-29 0.253316335988508 1102 30-31 0.26326040791008154 1102 32-33 0.2671603545045329 1102 34-35 0.3833792235324154 1102 36-37 0.40954063711301103 1102 38-39 0.3700358759573348 1102 40-41 0.30153375532302107 1102 42-43 0.17353689466994382 1102 44-45 0.374663620348322 1102 46-47 0.4442152597249347 1102 48-49 0.4014418411553109 1102 50-51 0.3883895464351781 1102 52-53 0.13682494724346128 1102 54-55 0.14371294512091737 1102 56-57 0.01915109264978554 1102 58-59 -0.12390210445751393 1102 60-61 -0.060413597629917604 1102 62-63 0.12251557142161573 1102 64-65 0.18359062870456455 1102 66-67 0.09923545735263062 1102 68-69 0.054060763542747026 1102 70-71 0.14004473617082525 1102 72-73 0.2530260665198618 1102 74-75 -0.08445714152888328 1102 76-77 0.013099229801042611 1102 78-79 -0.12918244731172734 1102 80-81 -0.17390052864829286 1102 82-83 -0.21685534938989903 1102 84-85 -0.4363455466441586 1102 86-87 -0.3591250694208128 1102 88-89 -0.3840409527950577 1102 90-91 -0.41365719273796486 1102 92-93 -0.3241066534668171 1102 94-95 -0.3531294264960998 1102 96-97 -0.4713680133071456 1102 98-99 -0.7246586285856544 1102 100-101 -0.6088594129292417 1103 1 0.17988409771835734 1103 2 0.23486929188969796 1103 3 0.20065398069171891 1103 4 0.3055806055947201 1103 5 0.1156777346109692 1103 6 0.37456971155898344 1103 7 0.35672901555502534 1103 8 0.19695473267667296 1103 9 0.3172907070611686 1103 10-11 0.26385148499029754 1103 12-13 0.2938916284214841 1103 14-15 0.4772703879310001 1103 16-17 0.6007032647692938 1103 18-19 0.5804482699646556 1103 20-21 0.6202756153295468 1103 22-23 0.5841957575376 1103 24-25 0.41468396550903464 1103 26-27 0.5359485713381673 1103 28-29 0.5043682666831373 1103 30-31 0.34289687854664663 1103 32-33 0.436770433221092 1103 34-35 0.29487324305566176 1103 36-37 0.3772464856301596 1103 38-39 0.4438477561882834 1103 40-41 0.4625039242361382 1103 42-43 0.5813627515010324 1103 44-45 0.7092591496730805 1103 46-47 0.6736548785405319 1103 48-49 0.39130681216528274 1103 50-51 0.202057032411453 1103 52-53 0.3736084440761829 1103 54-55 0.3892644444091573 1103 56-57 0.37750055840675856 1103 58-59 0.44198525134761724 1103 60-61 0.40922361014361286 1103 62-63 0.42339614798355996 1103 64-65 0.2671876999748335 1103 66-67 0.24222250549371793 1103 68-69 0.06023851505750244 1103 70-71 0.33515794107315244 1103 72-73 0.24292577964065742 1103 74-75 0.2868182020857013 1103 76-77 0.4084892235897577 1103 78-79 0.2870923887659309 1103 80-81 0.24648022071788844 1103 82-83 0.4461011619462987 1103 84-85 0.3331453817375447 1103 86-87 0.27732472188483825 1103 88-89 0.2814783178985536 1103 90-91 0.3668765910948082 1103 92-93 0.291436543967464 1103 94-95 0.3071528486227493 1103 96-97 0.33682925179389045 1103 98-99 0.40690340088532295 1103 100-101 0.3351683275888817 1104 1 -0.02874563782390993 1104 2 0.013491701078361729 1104 3 0.016619523196386154 1104 4 0.08022196876915899 1104 5 0.18772769561586244 1104 6 0.11235833444084165 1104 7 0.03576500922127224 1104 8 0.06684814604845002 1104 9 0.1140222080183193 1104 10-11 0.009491713761597964 1104 12-13 0.16513450886012038 1104 14-15 0.08880112316603572 1104 16-17 0.1820831007535091 1104 18-19 0.16760233609090136 1104 20-21 0.21263198334883526 1104 22-23 0.22405643865214842 1104 24-25 -0.027077431697492216 1104 26-27 -0.06645199113673073 1104 28-29 0.2833936253199596 1104 30-31 0.2605067147671889 1104 32-33 0.18811458277324533 1104 34-35 0.03940683793194211 1104 36-37 0.16422507377092188 1104 38-39 0.17993063851759672 1104 40-41 -0.025827085146310935 1104 42-43 0.22138000388208923 1104 44-45 0.10954393212764302 1104 46-47 0.1766332776141084 1104 48-49 -0.03615283380640477 1104 50-51 0.006293319131842168 1104 52-53 -0.07474312636315972 1104 54-55 -0.117437396632603 1104 56-57 0.016176615410508077 1104 58-59 0.02305608064357756 1104 60-61 0.07774510471974594 1104 62-63 0.06386834328996827 1104 64-65 -0.025867034976599257 1104 66-67 -0.0937250756279262 1104 68-69 -0.22873118692534078 1104 70-71 -0.3207462727618413 1104 72-73 -0.2007516953670212 1104 74-75 -0.5538572404496911 1104 76-77 -0.43763135401050945 1104 78-79 -0.24549156888301837 1104 80-81 -0.13997784181607997 1104 82-83 -0.6027224856324587 1104 84-85 -0.6824048037846318 1104 86-87 -0.578070192207754 1104 88-89 -0.5714333168236365 1104 90-91 -0.5863470044904737 1104 92-93 -0.7247192473968909 1104 94-95 -0.8067063672210821 1104 96-97 -0.7185550272692822 1104 98-99 -0.5450357194795785 1104 100-101 -0.04610220392625308 1105 1 0.19205486991739562 1105 2 0.2250765876226346 1105 3 0.25109818583372956 1105 4 0.2730279964503737 1105 5 0.35295731463519786 1105 6 0.2622957401855359 1105 7 0.401699177532862 1105 8 0.30995782865190336 1105 9 0.46500013408140717 1105 10-11 0.3770172318382379 1105 12-13 0.32477285963250324 1105 14-15 0.5443947205015576 1105 16-17 0.33214423932845705 1105 18-19 0.13652356049276904 1105 20-21 0.25748003943328257 1105 22-23 0.25944926855298434 1105 24-25 0.3212887126710626 1105 26-27 0.315982896280957 1105 28-29 0.4356735118933486 1105 30-31 0.3066145166316261 1105 32-33 0.180348528074596 1105 34-35 0.35279938834215585 1105 36-37 0.2908788722232458 1105 38-39 0.30214519415660135 1105 40-41 0.4323293830237773 1105 42-43 0.368379846668617 1105 44-45 0.37427687300955625 1105 46-47 0.19673447651244658 1105 48-49 0.30284717201632105 1105 50-51 0.2847734141586571 1105 52-53 0.36504964298137565 1105 54-55 0.5245170580404235 1105 56-57 0.4582763469830482 1105 58-59 0.5606755192167796 1105 60-61 0.42333497551356203 1105 62-63 0.5561977812226715 1105 64-65 0.37530566111810515 1105 66-67 0.37266850540397556 1105 68-69 0.5233728534610584 1105 70-71 0.5244555136490909 1105 72-73 0.46507882850322346 1105 74-75 0.4218969475426917 1105 76-77 0.5597545352063982 1105 78-79 0.42943433698686917 1105 80-81 0.45857695872659576 1105 82-83 0.3100633372054169 1105 84-85 0.15643362151858398 1105 86-87 0.302950647312219 1105 88-89 0.42356787683425523 1105 90-91 0.487597190547401 1105 92-93 0.46449707252671146 1105 94-95 0.49130167977992656 1105 96-97 0.6401170428828671 1105 98-99 0.7907594604716266 1105 100-101 0.8988040793724323 1106 1 -0.14130246087906073 1106 2 -0.06913402439838023 1106 3 -0.07249046336621134 1106 4 -0.0933978385327876 1106 5 -0.11234663894684616 1106 6 0.07805007472731518 1106 7 -0.030532843653467978 1106 8 -0.13770627542852054 1106 9 -0.07676316522695714 1106 10-11 -0.08889865705351241 1106 12-13 -0.03625354614745646 1106 14-15 -0.07705644037572768 1106 16-17 -0.18102265445705257 1106 18-19 -0.165811049848422 1106 20-21 -0.11815825023323612 1106 22-23 -0.09167389842650664 1106 24-25 -0.131796246918924 1106 26-27 -0.28270409966518883 1106 28-29 -0.09789979607123911 1106 30-31 -0.09553582747484768 1106 32-33 -0.19655391366728026 1106 34-35 -0.10333410611700344 1106 36-37 0.03507020056852639 1106 38-39 -0.09041987753581537 1106 40-41 0.10756485813069361 1106 42-43 0.12394168727404065 1106 44-45 0.14371781782396198 1106 46-47 0.21170203708462054 1106 48-49 0.18910948142931971 1106 50-51 0.15474658842308742 1106 52-53 0.108558757732105 1106 54-55 0.07099500180064666 1106 56-57 0.1553666545791046 1106 58-59 0.01030484411672461 1106 60-61 0.20599080903972578 1106 62-63 0.14334199740552833 1106 64-65 -0.12778829752055287 1106 66-67 -0.2229013914926341 1106 68-69 0.1096283064560275 1106 70-71 0.04141743481742566 1106 72-73 -0.0823281599008574 1106 74-75 -0.10378719441944284 1106 76-77 -0.19768939214985437 1106 78-79 0.11046091417727766 1106 80-81 0.11293121270545825 1106 82-83 0.1321504308555177 1106 84-85 0.1636097200453115 1106 86-87 0.29867738477140193 1106 88-89 0.45191059488977814 1106 90-91 0.36011066769410505 1106 92-93 0.36446793275005973 1106 94-95 0.3297833260058951 1106 96-97 0.5132630245933711 1106 98-99 0.5764526871876612 1106 100-101 0.7533760246239325 1107 1 0.02695243146036219 1107 2 0.08077462975432326 1107 3 0.021403087533801823 1107 4 -0.09260053760158371 1107 5 0.09761463263170356 1107 6 0.105907924509836 1107 7 0.004179132771263028 1107 8 0.04715055139139679 1107 9 0.08620170751261469 1107 10-11 0.0397390284450978 1107 12-13 0.034654118309724424 1107 14-15 -0.013633036433020607 1107 16-17 0.029556275840100454 1107 18-19 0.24938230117189875 1107 20-21 0.1039183361211613 1107 22-23 0.1810512573050005 1107 24-25 -0.01558326000674981 1107 26-27 0.07622575393195063 1107 28-29 0.060740911186805135 1107 30-31 0.014274633357331368 1107 32-33 0.05344899720190455 1107 34-35 0.007329100773716846 1107 36-37 -0.07169770890066474 1107 38-39 0.006469928045845563 1107 40-41 -0.04664994183715265 1107 42-43 -0.11067880469057911 1107 44-45 -0.3252004754641291 1107 46-47 -0.15130192446973467 1107 48-49 -0.026464223164580858 1107 50-51 0.0948081879595648 1107 52-53 0.13273366493082506 1107 54-55 0.024452975780256736 1107 56-57 -0.027640800018261302 1107 58-59 -0.014632710808605509 1107 60-61 -0.06081807437857378 1107 62-63 -0.04109122864148418 1107 64-65 -3.7955326952499036E-4 1107 66-67 -0.07661080912474105 1107 68-69 -0.05416103532714445 1107 70-71 -0.09318060187061761 1107 72-73 0.07686228579479604 1107 74-75 0.010734438883115871 1107 76-77 0.0890073812209593 1107 78-79 -0.06899832949573792 1107 80-81 -0.04640339032128793 1107 82-83 0.032844326624221765 1107 84-85 -0.04405972719449025 1107 86-87 0.03129408983381765 1107 88-89 -0.030863990172750988 1107 90-91 -0.24876415661414697 1107 92-93 -0.06256794301958735 1107 94-95 -0.027853269995954832 1107 96-97 0.036428276023166006 1107 98-99 0.21834453037775248 1107 100-101 0.09132017474716037 1108 1 0.05107227558892191 1108 2 0.10868270540827041 1108 3 0.0011800293949058016 1108 4 0.01714855791091452 1108 5 0.04387325512876572 1108 6 0.1396790054941235 1108 7 0.16223177210810036 1108 8 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52-53 -2.769840159106117 2316 54-55 -2.3899159816169018 2316 56-57 -2.39539361500988 2316 58-59 -2.175441689557644 2316 60-61 -2.1006955430756484 2316 62-63 -2.4681793042370046 2316 64-65 -2.536334138969515 2316 66-67 -2.505225845579563 2316 68-69 -2.7110806931673643 2316 70-71 -2.5014880634762697 2316 72-73 -2.446225462855754 2316 74-75 -2.568987849676869 2316 76-77 -2.5895917874438332 2316 78-79 -2.56167585810228 2316 80-81 -2.3701679515871668 2316 82-83 -2.0907246612756154 2316 84-85 -2.4306826550780123 2316 86-87 -2.6143455099599677 2316 88-89 -2.7948252481072196 2316 90-91 -2.9456306904632257 2316 92-93 -3.131587121812366 2316 94-95 -3.2757755027561153 2316 96-97 -3.4578300954347228 2316 98-99 -3.6475979866692754 2316 100-101 -3.6086347561628855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6845.0 3 1608.0 4 1448.0 5 1598.0 6 1878.0 7 2280.0 8 2887.0 9 3619.0 10 4594.0 11 6132.0 12 8548.0 13 11472.0 14 12096.0 15 12994.0 16 16639.0 17 21562.0 18 17308.0 19 11661.0 20 11592.0 21 12097.0 22 12794.0 23 14038.0 24 15857.0 25 17924.0 26 20417.0 27 23550.0 28 27309.0 29 31791.0 30 36789.0 31 43470.0 32 52309.0 33 65022.0 34 84378.0 35 120330.0 36 161487.0 37 230318.0 38 213705.0 39 36277.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.483742372131566 20.045314286005322 15.141578040178958 20.329365301684152 2 15.165940182281537 23.048471474430052 39.20543572703576 22.58015261625265 3 19.656063421713096 27.396272287607932 31.693116615337193 21.254547675341783 4 12.287235899106479 18.117088607594937 38.729828857967235 30.865846635331344 5 12.047507527601205 39.060395362706736 34.21102011724148 14.681076992450578 6 27.758253577216273 37.49809239355556 18.41403416977334 16.329619859454823 7 24.820915838256315 35.82228281526044 22.444149069532692 16.912652276950556 8 24.87877625061048 32.76696841787018 21.359435684550803 20.994819646968534 9 26.05715946874035 17.703155943967005 22.834135794618362 33.40554879267428 10-11 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31.837405612725373 19.96232341605885 36-37 20.546290940343745 26.996823126036677 32.40710700098354 20.049778932636038 38-39 20.15229893235318 27.723389645482293 32.21949551845195 19.904815903712574 40-41 20.109423206415837 27.869760193998395 31.97354991818246 20.047266681403308 42-43 19.80888842508095 28.061977714352853 30.930512245867508 21.19862161469869 44-45 20.18843743408631 27.466951012397793 31.041343256240317 21.30326829727558 46-47 20.820917144886064 28.12740158825036 30.642934139646634 20.40874712721694 48-49 20.587808052487087 28.03439136252251 30.411544472483044 20.966256112507363 50-51 19.63706378279589 28.544021872808102 30.889174025602458 20.92974031879355 52-53 20.354430915643004 29.043561345665214 29.567758808583072 21.03424893010871 54-55 19.689122574486067 28.152456496574757 30.114581037309087 22.04383989163009 56-57 20.56426794354037 28.24327829831575 29.563958186861722 21.628495571282162 58-59 21.204998405990267 27.674197541942153 29.688413044762857 21.432391007304723 60-61 20.382574374977438 27.972473956405675 30.71596228296093 20.928989385655957 62-63 20.022009345214684 27.977117870406115 30.590326250595663 21.410546533783542 64-65 19.732034949487353 28.671844287382402 30.0564569203438 21.53966384278644 66-67 20.113690951593192 27.999271408401142 29.32927959958124 22.55775804042442 68-69 19.676753059576498 27.848534718034163 29.89156227138053 22.58314995100881 70-71 20.36263053892631 27.797659051508095 29.48694875401423 22.352761655551365 72-73 20.100992255848997 27.13650380199269 30.310269760433577 22.452234181724737 74-75 19.489697707191 28.5005951105672 29.505111089130807 22.504596093110987 76-77 19.796732584494357 27.47733759169969 29.61280954745978 23.113120276346176 78-79 19.864987966461506 27.476342431785323 29.751022312664784 22.907647289088388 80-81 20.149139378443635 27.380746506837472 29.412103792659856 23.05801032205904 82-83 19.851811903431805 27.08523150832436 29.885341412428286 23.17761517581555 84-85 19.67714498701202 27.208020401290078 29.60128234178437 23.513552269913536 86-87 19.742382144100475 27.152307489076165 29.49614244666169 23.60916792016167 88-89 19.7418829290689 26.769116900492957 29.151747246438592 24.337252923999554 90-91 19.89753461135857 26.258827119129062 29.386550806393924 24.457087463118445 92-93 20.357695161614693 26.508247442623034 29.400409297110503 23.73364809865177 94-95 19.987261309238104 25.974080961862406 29.482075494080522 24.556582234818965 96-97 19.5823998212913 25.728650406219177 29.977435106768958 24.711514665720564 98-99 20.02995783347343 25.949111831263817 29.10417391310695 24.916756422155803 100-101 20.099488035489006 24.973861673604652 29.618838425547644 25.307811865358698 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6611.0 1 4169.0 2 2026.5 3 2242.5 4 1839.0 5 1762.0 6 2685.5 7 4663.0 8 6739.5 9 7204.0 10 5725.0 11 4357.0 12 4134.5 13 4159.5 14 4287.0 15 4324.0 16 4393.0 17 4588.0 18 4665.0 19 4769.5 20 5086.0 21 5630.5 22 6235.5 23 6975.0 24 7762.0 25 8805.5 26 10468.0 27 12493.5 28 14490.0 29 16433.0 30 18554.0 31 20863.0 32 23615.5 33 26261.0 34 29087.0 35 31582.5 36 34141.5 37 37047.0 38 37960.5 39 37568.5 40 37461.0 41 38427.0 42 39358.0 43 40443.0 44 41984.5 45 43344.5 46 46258.0 47 54278.5 48 69654.0 49 70486.5 50 56141.5 51 46239.0 52 40089.0 53 37460.5 54 35298.5 55 32077.0 56 28546.5 57 24952.0 58 22390.5 59 19866.0 60 16777.5 61 14004.5 62 11508.5 63 8827.0 64 6440.5 65 4906.5 66 3649.0 67 2882.0 68 2284.0 69 1631.0 70 1240.5 71 983.5 72 803.5 73 713.5 74 582.0 75 441.5 76 317.5 77 211.0 78 151.5 79 105.0 80 76.5 81 58.5 82 45.0 83 38.0 84 24.5 85 13.0 86 11.5 87 13.0 88 11.5 89 7.5 90 2.5 91 3.0 92 2.5 93 1.5 94 1.5 95 0.0 96 0.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16198966898173875 2 0.0934164638163749 3 0.08738725192153889 4 0.10162490892621188 5 0.122836112218888 6 0.04075166112051813 7 0.043729946514352795 8 0.048161054051521435 9 0.04765256630135454 10-11 0.046526629140270705 12-13 0.04986812006993886 14-15 0.047688886854937884 16-17 0.046780873015354156 18-19 0.05157518608835628 20-21 0.052882726017356864 22-23 0.0650137909141956 24-25 0.0799778589905356 26-27 0.08201180999120317 28-29 0.0720599783093654 30-31 0.06138173555586064 32-33 0.05190207107060643 34-35 0.3022233263670512 36-37 0.4775063179602958 38-39 0.4528446620772015 40-41 0.42843725006919064 42-43 0.45996349057953806 44-45 0.4698426811542091 46-47 0.46809929458220834 48-49 0.48625957137388304 50-51 0.47420114758421106 52-53 0.4817558227295477 54-55 0.4728209665480437 56-57 0.4504111849871171 58-59 0.42669386349718985 60-61 0.39375112139709184 62-63 0.45792953957887045 64-65 0.495811876966304 66-67 0.4986812006993886 68-69 0.5082698268453928 70-71 0.5064901197198087 72-73 0.4956302741983872 74-75 0.4879666373923005 76-77 0.4696610783862924 78-79 0.4421300987701134 80-81 0.44805034900419943 82-83 0.46094414552628843 84-85 0.4594913233829545 86-87 0.4694068345112089 88-89 0.4817921432831311 90-91 0.4870586235527168 92-93 0.48825720182096727 94-95 0.4931241560011361 96-97 0.49425009316221996 98-99 0.48709494410630005 100-101 0.473365774851794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1376631.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.75710900758688 #Duplication Level Percentage of deduplicated Percentage of total 1 80.72217211400238 45.00834949889621 2 11.56621986999713 12.897979641942946 3 3.298760949125791 5.517881214911326 4 1.407066757722547 3.1381589836515165 5 0.721716844494338 2.012042238554122 6 0.44613802513873546 1.4925219900054008 7 0.30271570865574987 1.1814986936079293 8 0.21296943501224233 0.949964800260943 9 0.1615094480963915 0.8104769912939128 >10 1.0287161186840432 10.903733491490472 >50 0.08557718440525827 3.273990659039762 >100 0.04094354952097512 4.062449317320657 >500 0.0013080940820227602 0.5232395949769493 >1k 0.0034010446132591767 4.1066358231359255 >5k 3.9242822460682804E-4 1.5835036028079934 >10k+ 3.9242822460682804E-4 2.5375734581039895 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13454 0.9773134558207682 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11174 0.8116917314806946 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10164 0.7383242132423286 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7524 0.5465516903222432 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7327 0.5322413922104036 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6860 0.49831799516355507 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4546 0.33022647317981363 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4485 0.325795365642645 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3954 0.2872229377371278 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3216 0.23361380064810394 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3142 0.2282383587177682 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2823 0.20506584553159124 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2673 0.1941696794565864 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2666 0.19366119170641952 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2526 0.18349143670308166 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2310 0.16780095755507465 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2288 0.1662028531974073 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2039 0.14811521751289924 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1986 0.1442652388330642 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1910 0.13874451468839508 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1854 0.1346766126870599 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1613 0.11717010585988548 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1586 0.1152087959663846 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1390 0.1009711389617116 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.264110716669899E-5 0.0 0.0 0.0 0.0 8 7.264110716669899E-5 0.0 0.0 0.0 0.0 9 7.264110716669899E-5 0.0 0.0 0.0 0.0 10-11 1.4528221433339798E-4 0.0 0.0 7.264110716669899E-5 0.0 12-13 1.4528221433339798E-4 0.0 0.0 2.9056442866679595E-4 0.0 14-15 1.4528221433339798E-4 0.0 0.0 3.6320553583349494E-4 0.0 16-17 1.4528221433339798E-4 0.0 0.0 5.448083037502424E-4 0.0 18-19 2.1792332150009696E-4 0.0 0.0 7.627316252503393E-4 0.0 20-21 2.9056442866679595E-4 0.0 0.0 0.0013438604825839313 0.0 22-23 2.9056442866679595E-4 0.0 0.0 0.0035957348047516 0.0 24-25 3.6320553583349494E-4 0.0 0.0 0.00791788068117019 0.0 26-27 3.6320553583349494E-4 0.0 0.0 0.014491900879756449 0.0 28-29 3.6320553583349494E-4 0.0 0.0 0.034940372547182214 0.0 30-31 3.6320553583349494E-4 0.0 0.0 0.08804102188603918 0.0 32-33 3.6320553583349494E-4 0.0 0.0 0.18817678811533373 0.0 34-35 4.3584664300019393E-4 0.0 0.0 0.33149769255523087 0.0 36-37 5.08487750166893E-4 0.0 0.0 0.5350017542827381 0.0 38-39 5.811288573335919E-4 0.0 0.0 0.8072606239435258 0.0 40-41 5.811288573335919E-4 0.0 0.0 1.0937571506089867 0.0 42-43 6.537699645002908E-4 0.0 0.0 1.375931531398029 0.0 44-45 7.264110716669899E-4 0.0 0.0 1.7097537393825941 0.0 46-47 7.990521788336889E-4 0.0 0.0 2.095804903420016 0.0 48-49 7.990521788336889E-4 0.0 0.0 2.472739608508017 0.0 50-51 7.990521788336889E-4 0.0 0.0 2.874735495568529 0.0 52-53 7.990521788336889E-4 0.0 0.0 3.264927202714453 0.0 54-55 7.990521788336889E-4 0.0 0.0 3.6991394208033963 0.0 56-57 7.990521788336889E-4 0.0 0.0 4.217760605420043 0.0 58-59 8.353727324170384E-4 0.0 0.0 4.737108201108358 0.0 60-61 8.716932860003879E-4 0.0 0.0 5.308575791188779 0.0 62-63 8.716932860003879E-4 0.0 0.0 5.867004302532777 0.0 64-65 8.716932860003879E-4 0.0 0.0 6.438689815934699 0.0 66-67 9.443343931670869E-4 0.0 0.0 7.098597953990575 0.0 68-69 9.443343931670869E-4 0.0 0.0 7.827297220533317 0.0 70-71 9.443343931670869E-4 0.0 0.0 8.604629708324161 0.0 72-73 9.443343931670869E-4 0.0 0.0 9.369141040700086 0.0 74-75 9.443343931670869E-4 0.0 0.0 10.169682362230692 0.0 76-77 9.443343931670869E-4 0.0 0.0 10.9745821501913 0.0 78-79 9.443343931670869E-4 0.0 0.0 11.920442006608887 0.0 80-81 9.443343931670869E-4 0.0 0.0 12.911775196112828 0.0 82-83 9.443343931670869E-4 0.0 0.0 13.866497267604753 0.0 84-85 9.443343931670869E-4 0.0 0.0 14.855687544447278 0.0 86-87 9.443343931670869E-4 0.0 0.0 15.84800138889797 0.0 88-89 9.443343931670869E-4 0.0 0.0 16.88095793280843 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 8775 0.0 23.392448 12-13 GTACATG 9420 0.0 22.948433 1 TACATGG 9420 0.0 22.432932 2 CATGGGG 2515 0.0 21.853556 4 AGTACTT 8985 0.0 21.739 12-13 ACATGGG 9530 0.0 21.179651 3 GTACTTT 9790 0.0 20.773714 14-15 GTATAGG 450 0.0 18.962652 1 CATGGGA 6265 0.0 18.755674 4 ATGGGAG 1780 0.0 18.100517 5 ACTTTTT 11490 0.0 18.092306 16-17 AGAGTAC 16010 0.0 18.085186 10-11 TACTTTT 11100 0.0 17.46886 14-15 TATAGGG 245 3.85402E-6 17.40518 2 GAGTAGG 465 1.2732926E-11 16.311958 1 GTAAGGT 320 1.6500417E-7 16.28714 3 CCGACGC 280 0.0 16.13724 94-95 ATGGGGA 1430 0.0 15.904035 5 GCCGTAT 255 3.6379788E-12 15.854129 94-95 GTATCAA 26385 0.0 15.811193 1 >>END_MODULE