##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938795_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1376631 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.930571809003283 31.0 31.0 34.0 30.0 34.0 2 32.128381534339994 33.0 31.0 34.0 30.0 34.0 3 32.195642114698856 34.0 31.0 34.0 30.0 34.0 4 35.860011869556914 37.0 35.0 37.0 35.0 37.0 5 35.77728309183797 37.0 35.0 37.0 35.0 37.0 6 35.82687662852282 37.0 35.0 37.0 35.0 37.0 7 35.75898043847625 37.0 35.0 37.0 33.0 37.0 8 35.740243391293674 37.0 35.0 37.0 33.0 37.0 9 37.350852189148725 39.0 37.0 39.0 34.0 39.0 10-11 37.28177340187749 39.0 37.0 39.0 33.0 39.0 12-13 37.217422461066185 39.0 37.0 39.0 33.0 39.0 14-15 38.34231903828986 40.0 38.0 41.0 33.0 41.0 16-17 38.29691435104977 40.0 38.0 41.0 33.0 41.0 18-19 38.3666781439616 40.0 38.0 41.0 33.5 41.0 20-21 38.42768795704877 40.0 38.0 41.0 34.0 41.0 22-23 38.36289753753911 40.0 38.0 41.0 34.0 41.0 24-25 38.26056437781802 40.0 37.5 41.0 33.0 41.0 26-27 38.05949234035846 40.0 37.0 41.0 33.0 41.0 28-29 37.87902894820762 40.0 37.0 41.0 33.0 41.0 30-31 37.5900931331635 40.0 36.5 41.0 32.5 41.0 32-33 37.260436892674946 39.0 35.5 41.0 32.0 41.0 34-35 37.10641994841029 39.0 35.0 41.0 31.0 41.0 36-37 36.942648393069746 39.0 35.0 41.0 31.0 41.0 38-39 36.78915373836561 39.0 35.0 41.0 31.0 41.0 40-41 36.60178471936198 39.0 35.0 41.0 30.5 41.0 42-43 36.33556958981746 39.0 35.0 41.0 30.0 41.0 44-45 35.96999994915122 39.0 35.0 41.0 29.5 41.0 46-47 35.50575753415403 39.0 35.0 41.0 27.5 41.0 48-49 35.08391645982111 39.0 35.0 41.0 24.5 41.0 50-51 34.78070993606856 39.0 35.0 40.5 24.0 41.0 52-53 34.57922093865386 38.5 34.0 40.0 23.5 41.0 54-55 34.33115773217369 38.0 34.0 40.0 23.0 41.0 56-57 34.03283305402827 38.0 34.0 40.0 22.0 41.0 58-59 33.7165580318909 38.0 33.5 40.0 20.0 41.0 60-61 33.32876166525379 37.0 33.0 40.0 18.5 41.0 62-63 32.95219379775699 37.0 33.0 40.0 15.5 41.0 64-65 32.580504507017494 36.0 32.0 39.0 12.0 41.0 66-67 32.1370650522907 36.0 32.0 39.0 7.5 40.0 68-69 31.673968187553527 35.0 31.0 38.5 4.5 40.0 70-71 31.17176607238977 35.0 31.0 37.5 2.0 40.0 72-73 30.82597442597181 35.0 31.0 37.0 2.0 39.5 74-75 30.417355485965373 35.0 30.5 37.0 2.0 39.0 76-77 28.395752020693998 32.5 27.5 35.0 2.0 37.0 78-79 29.407207523294186 34.0 29.0 36.0 2.0 37.5 80-81 29.43261375052574 34.0 30.0 35.0 2.0 37.0 82-83 29.11621778094493 34.0 29.5 35.0 2.0 37.0 84-85 28.732406505446992 34.0 29.0 35.0 2.0 36.0 86-87 28.23545706874246 34.0 29.0 35.0 2.0 36.0 88-89 27.736252488865937 34.0 27.5 35.0 2.0 35.5 90-91 27.32325837497485 33.0 26.5 35.0 2.0 35.0 92-93 27.055841398312253 33.0 26.0 35.0 2.0 35.0 94-95 26.692730659123615 33.0 25.0 35.0 2.0 35.0 96-97 26.198767135129167 33.0 24.5 35.0 2.0 35.0 98-99 25.532752059193783 33.0 21.5 35.0 2.0 35.0 100-101 22.72559967049994 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03438444196915569 1101 2 0.04903552137429301 1101 3 0.16168302132820855 1101 4 0.049999040972025455 1101 5 0.12644546011132007 1101 6 0.14558464114876557 1101 7 0.11481188835124811 1101 8 0.1849089915628781 1101 9 0.2577916173351582 1101 10-11 0.13319205852916838 1101 12-13 0.14383191580639476 1101 14-15 0.2007326309327766 1101 16-17 0.25330199903293504 1101 18-19 0.29788888596532814 1101 20-21 0.3126251285017716 1101 22-23 0.15623911591254114 1101 24-25 0.03366344219390527 1101 26-27 0.21897249727990697 1101 28-29 0.15253561587260833 1101 30-31 0.15229309709901884 1101 32-33 0.14427021156829056 1101 34-35 0.16746750236059427 1101 36-37 0.10011249613258855 1101 38-39 0.10143789032635908 1101 40-41 -0.006105772032874768 1101 42-43 -0.10296131309647194 1101 44-45 -0.20658538506594937 1101 46-47 -0.2913441016470202 1101 48-49 -0.4331157762307143 1101 50-51 -0.441425745117364 1101 52-53 -0.2492332823169292 1101 54-55 -0.37831805336847424 1101 56-57 -0.5563768989847873 1101 58-59 -0.4122579063875378 1101 60-61 -0.5001949351477961 1101 62-63 -0.5524360055679196 1101 64-65 -0.7065281171707412 1101 66-67 -0.7607282551817818 1101 68-69 -0.7674320850812784 1101 70-71 -0.685785610402025 1101 72-73 -0.4634859795235684 1101 74-75 -0.4892837772063423 1101 76-77 -0.6111564680272501 1101 78-79 -0.6701837101534025 1101 80-81 -0.48094453970152884 1101 82-83 -0.5238142829690098 1101 84-85 -0.5126724766556521 1101 86-87 -0.195117501240766 1101 88-89 -0.07771610080321878 1101 90-91 0.07537627832244809 1101 92-93 -0.03334960259066477 1101 94-95 -0.06642676530379887 1101 96-97 -0.05557524642991041 1101 98-99 -0.05945771031243652 1101 100-101 0.44273120789527454 1102 1 0.07198772949511678 1102 2 0.12812688572702058 1102 3 0.17231371963288922 1102 4 0.07320651449654036 1102 5 0.08013177534264315 1102 6 0.1855022320638824 1102 7 0.10857597301387756 1102 8 0.11510982228180211 1102 9 0.22611125264366194 1102 10-11 0.175428555664368 1102 12-13 0.16222758988178043 1102 14-15 0.2014009619831043 1102 16-17 0.2999783868785144 1102 18-19 0.19468144926346298 1102 20-21 0.2498461969477077 1102 22-23 0.21260354228934375 1102 24-25 0.12596677243413978 1102 26-27 0.1950156828807792 1102 28-29 0.2871500091090269 1102 30-31 0.2542458020907006 1102 32-33 0.31502971549358705 1102 34-35 0.49479874416755365 1102 36-37 0.378251442710301 1102 38-39 0.19249753565062377 1102 40-41 0.27676488473586147 1102 42-43 0.3213377678102489 1102 44-45 0.16091292109226885 1102 46-47 0.2751502350298125 1102 48-49 0.2683096284501758 1102 50-51 0.13028912239474977 1102 52-53 0.055264235836631315 1102 54-55 0.11385069009550364 1102 56-57 0.19752814277209296 1102 58-59 0.19137114962707358 1102 60-61 0.197036369994116 1102 62-63 0.06742999681818418 1102 64-65 -0.0076655687489619595 1102 66-67 0.21571261501102157 1102 68-69 0.08958005166330807 1102 70-71 0.13709009106940684 1102 72-73 0.17843955035433012 1102 74-75 0.19082775859263634 1102 76-77 0.171806245359992 1102 78-79 0.17472574295664245 1102 80-81 0.22938550168712624 1102 82-83 0.22033459835896352 1102 84-85 0.25165889771284 1102 86-87 0.23636475760771347 1102 88-89 0.41463583533158044 1102 90-91 0.4048938648188454 1102 92-93 0.50301460285025 1102 94-95 0.30628194546166654 1102 96-97 0.3849359255613116 1102 98-99 0.49583517702499336 1102 100-101 0.9428840218819907 1103 1 0.16315948724048823 1103 2 0.07787287558111444 1103 3 0.16747580517461103 1103 4 0.1348438527795679 1103 5 0.19880901327837108 1103 6 0.08931431772644771 1103 7 0.09333234320647676 1103 8 0.045052204756792946 1103 9 0.15453646224432305 1103 10-11 0.037096346162790894 1103 12-13 0.036917327726875726 1103 14-15 0.24529082163706306 1103 16-17 0.23815313909173597 1103 18-19 0.14866507032188991 1103 20-21 0.22758073338523133 1103 22-23 0.09845244613043036 1103 24-25 0.21738474600837776 1103 26-27 0.3652767985436114 1103 28-29 0.13261136017231223 1103 30-31 0.21705301298195678 1103 32-33 0.22836536899571058 1103 34-35 0.20850864572955885 1103 36-37 0.40656414829295784 1103 38-39 0.40015203834902735 1103 40-41 0.6161548253540374 1103 42-43 0.5112064245050192 1103 44-45 0.6149494105802162 1103 46-47 0.5069088142164517 1103 48-49 0.6811359323971189 1103 50-51 0.6730681408183443 1103 52-53 0.528520967347184 1103 54-55 0.5175250157360338 1103 56-57 0.6839092353299137 1103 58-59 0.38632967660991113 1103 60-61 0.40078253627770977 1103 62-63 0.4601170543849591 1103 64-65 0.5176599195136404 1103 66-67 0.3978041746719896 1103 68-69 0.5090686840446459 1103 70-71 0.3893842788049007 1103 72-73 0.3896815536267333 1103 74-75 0.2769664418485114 1103 76-77 0.4548484095941028 1103 78-79 0.31477330641688184 1103 80-81 0.34246925978052545 1103 82-83 0.3157621136273647 1103 84-85 0.24250208572143706 1103 86-87 0.5825807677391204 1103 88-89 0.7775519249771001 1103 90-91 0.8868004756081156 1103 92-93 0.8130686071957349 1103 94-95 0.8097597818075961 1103 96-97 0.7317395305235728 1103 98-99 0.8295691020013685 1103 100-101 1.08552576365204 1104 1 -0.11392320602162798 1104 2 -0.014407274727297192 1104 3 -0.03471382921670596 1104 4 -0.0010871849629694452 1104 5 0.012581376381909593 1104 6 0.051361442723496964 1104 7 -0.1361255066269038 1104 8 -0.07961042847649225 1104 9 -0.18539216489154597 1104 10-11 0.020638047294831097 1104 12-13 0.07207318999142842 1104 14-15 -0.050960921326087316 1104 16-17 0.05980015064847066 1104 18-19 0.1232975709718076 1104 20-21 -0.013494704423486326 1104 22-23 -0.023344637508166954 1104 24-25 0.0449985999067124 1104 26-27 -0.05572383955816207 1104 28-29 -0.07789191305340637 1104 30-31 -0.17833847426782512 1104 32-33 -0.030434527017341395 1104 34-35 0.013381852545428785 1104 36-37 -0.017042072017581233 1104 38-39 0.06650162358288014 1104 40-41 0.24631813213918718 1104 42-43 0.3360149939753967 1104 44-45 0.2639898945922212 1104 46-47 0.2836186906137712 1104 48-49 0.19181941028808325 1104 50-51 0.27358157640551184 1104 52-53 0.07823323071777821 1104 54-55 0.2196709283397169 1104 56-57 0.18519134721042718 1104 58-59 0.24024041326294565 1104 60-61 0.28787338306238297 1104 62-63 0.13253746891476936 1104 64-65 0.27945416714440086 1104 66-67 0.020924728638853196 1104 68-69 0.035548134804461284 1104 70-71 0.024387705647715308 1104 72-73 -0.12042822351031646 1104 74-75 -0.14543565679729653 1104 76-77 -0.04724137368762982 1104 78-79 -0.1871894473467357 1104 80-81 -0.31627568858049315 1104 82-83 -0.2056905132249014 1104 84-85 -0.1619835333554427 1104 86-87 -0.16821390529280578 1104 88-89 0.18175866994909384 1104 90-91 -0.06845263809216817 1104 92-93 0.07713697861822766 1104 94-95 -0.06382162343428988 1104 96-97 0.2652646882765062 1104 98-99 0.2090789453119335 1104 100-101 0.31554195253016104 1105 1 -0.004718647995296976 1105 2 0.01295835141905144 1105 3 -0.015018474001585957 1105 4 -0.008614216947172793 1105 5 0.0584917879900857 1105 6 0.002627011224902276 1105 7 0.07010131166342859 1105 8 0.06175476678846792 1105 9 0.022710734241456976 1105 10-11 -0.045204995426473715 1105 12-13 -0.02923109960744341 1105 14-15 -0.057307105405605796 1105 16-17 -0.11783914340478674 1105 18-19 -0.11851422702634551 1105 20-21 -0.09354256719358744 1105 22-23 -0.0754830319509594 1105 24-25 -0.08033926350388754 1105 26-27 0.02625629331635082 1105 28-29 0.19111519648944864 1105 30-31 0.051306165043698115 1105 32-33 0.11926589396542653 1105 34-35 0.15705488344661234 1105 36-37 0.07909746367861459 1105 38-39 0.19053432398639103 1105 40-41 0.10110120665255096 1105 42-43 0.020062484091326382 1105 44-45 0.08109559445421866 1105 46-47 0.25628625397953186 1105 48-49 0.1476971564290679 1105 50-51 0.07211803537570916 1105 52-53 0.1455791178385013 1105 54-55 0.19709157920352283 1105 56-57 0.0781973853725404 1105 58-59 -0.01339325604141095 1105 60-61 -0.08982297366081582 1105 62-63 -0.009814968767557275 1105 64-65 -0.05876355605507655 1105 66-67 -0.04138683353907879 1105 68-69 -0.06648545065270994 1105 70-71 -0.025386663897123185 1105 72-73 -0.20300140056180638 1105 74-75 -0.08694525555356236 1105 76-77 -0.07615060328335588 1105 78-79 -0.034466160536943846 1105 80-81 0.01196829509259345 1105 82-83 0.1177599150362596 1105 84-85 0.14990326129463583 1105 86-87 0.30233331406657626 1105 88-89 0.2058172814629451 1105 90-91 0.14566775249598862 1105 92-93 0.09532395784858139 1105 94-95 0.2903538500985299 1105 96-97 0.21758329594736026 1105 98-99 0.47621288537283846 1105 100-101 0.790314429690536 1106 1 -0.1759611166486117 1106 2 -0.1657867526699448 1106 3 -0.16841025829867817 1106 4 -0.190374430867422 1106 5 -0.23182780679560722 1106 6 -0.18629473133374574 1106 7 -0.0851262785627469 1106 8 -0.10789187056700911 1106 9 -0.23065619554025574 1106 10-11 -0.20483440603164382 1106 12-13 -0.13672687862509747 1106 14-15 -0.07979701361531255 1106 16-17 -0.3234416939922937 1106 18-19 -0.3331681064733729 1106 20-21 -0.25661732786173985 1106 22-23 -0.24344641867865136 1106 24-25 -0.2088953875707773 1106 26-27 -0.3007434238564102 1106 28-29 -0.28672486041882195 1106 30-31 -0.22882307843627814 1106 32-33 -0.2413474126278814 1106 34-35 -0.07696751989931983 1106 36-37 0.037245030935785906 1106 38-39 -0.056216162390761326 1106 40-41 -0.2158427164184289 1106 42-43 -0.06574044531328127 1106 44-45 0.17008675824548192 1106 46-47 0.04592534927517988 1106 48-49 0.1524135149854473 1106 50-51 0.24926130880108133 1106 52-53 0.20876615235199125 1106 54-55 0.19529776883489802 1106 56-57 0.043448201714817 1106 58-59 0.09166849457352555 1106 60-61 -0.04695664810094513 1106 62-63 -0.13982293935352175 1106 64-65 0.13744227361436145 1106 66-67 0.16567270588104321 1106 68-69 0.03168988509751003 1106 70-71 0.05370983161620657 1106 72-73 0.33662355337163774 1106 74-75 0.4909110853475447 1106 76-77 0.19021180983297015 1106 78-79 0.3330395602868492 1106 80-81 0.40634346114646647 1106 82-83 0.45242767872981204 1106 84-85 0.2777840281849926 1106 86-87 0.22518318422315886 1106 88-89 0.3136096005606426 1106 90-91 0.16173148290162942 1106 92-93 0.19667124399261482 1106 94-95 0.16509309195010147 1106 96-97 0.2178806930796675 1106 98-99 0.46549458987836445 1106 100-101 0.83027449105548 1107 1 -0.09552631595998307 1107 2 -0.17212024956640448 1107 3 -0.2659166136963975 1107 4 -0.13179443602381724 1107 5 -0.22225908671865113 1107 6 -0.042290983243134406 1107 7 -0.06588293821560853 1107 8 -0.13103854787913605 1107 9 -0.21667348887547178 1107 10-11 -0.26702249253713006 1107 12-13 -0.2966957739086524 1107 14-15 -0.4033767173719909 1107 16-17 -0.2849738646413016 1107 18-19 -0.23171793916770156 1107 20-21 -0.17237899143734836 1107 22-23 -0.23540299405491538 1107 24-25 -0.3343763047440689 1107 26-27 -0.4058244153261725 1107 28-29 -0.2826282971850418 1107 30-31 -0.4950453524072813 1107 32-33 -0.38939283327619734 1107 34-35 -0.4229104613187715 1107 36-37 -0.361160367201812 1107 38-39 -0.37252007931177644 1107 40-41 -0.30590611792155187 1107 42-43 -0.35922065278894877 1107 44-45 -0.558920191801235 1107 46-47 -0.5832545895688455 1107 48-49 -0.9044909281098299 1107 50-51 -0.6101096107840718 1107 52-53 -0.4140622699291825 1107 54-55 -0.594291022378151 1107 56-57 -0.7607986178140678 1107 58-59 -0.6558094451964323 1107 60-61 -0.7321539080027577 1107 62-63 -0.8545727293551053 1107 64-65 -0.6583998962861415 1107 66-67 -0.7272682977312073 1107 68-69 -0.7071663373705199 1107 70-71 -0.8204843427642636 1107 72-73 -0.6810432047794919 1107 74-75 -0.7890940156041744 1107 76-77 -0.7740363403789665 1107 78-79 -0.7452728009270615 1107 80-81 -0.6808631301702697 1107 82-83 -0.47935152768408074 1107 84-85 -0.5278406027583777 1107 86-87 -0.5258130261023872 1107 88-89 -0.3166318352463797 1107 90-91 -0.41842664660155293 1107 92-93 -0.43983687733241084 1107 94-95 -0.31564554803636824 1107 96-97 -0.3183197077886071 1107 98-99 -0.5638918820650147 1107 100-101 -0.029391119381511288 1108 1 -0.05386481538608123 1108 2 -0.08702898133630299 1108 3 -0.06385152465247046 1108 4 -0.0645688912427076 1108 5 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-0.6966286287254562 2316 64-65 -0.888632397081814 2316 66-67 -0.7685462415943931 2316 68-69 -0.8180826463079427 2316 70-71 -0.8059983061911744 2316 72-73 -0.90008172172789 2316 74-75 -0.8763024763554803 2316 76-77 -0.8948046062601129 2316 78-79 -0.9614083113972924 2316 80-81 -0.8989621369607725 2316 82-83 -1.1183789394625947 2316 84-85 -1.2318195595573478 2316 86-87 -1.6398469562320557 2316 88-89 -1.7724664102247694 2316 90-91 -2.0928944820203768 2316 92-93 -2.120448858039005 2316 94-95 -2.0787641007477973 2316 96-97 -2.2126532400591223 2316 98-99 -2.8327981826507305 2316 100-101 -3.6479317301932532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 29.0 5 80.0 6 242.0 7 530.0 8 902.0 9 1636.0 10 2737.0 11 4176.0 12 5873.0 13 8135.0 14 9282.0 15 11963.0 16 15135.0 17 20545.0 18 18910.0 19 11854.0 20 10420.0 21 10518.0 22 11108.0 23 11784.0 24 13268.0 25 14943.0 26 17024.0 27 20399.0 28 24285.0 29 29830.0 30 35438.0 31 42887.0 32 51716.0 33 64466.0 34 84998.0 35 121559.0 36 170120.0 37 267069.0 38 237251.0 39 25513.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09608084202175 20.97988429055409 15.608962158898498 20.315072708525662 2 15.360761162577335 23.64148417404519 38.50835844899614 22.48939621438134 3 19.51822964904902 27.546742736434094 32.06422055002393 20.870807064492954 4 12.495577972601227 18.930272527641755 37.978078366679235 30.596071133077785 5 11.921059456019805 39.6098155569648 33.99676456508679 14.472360421928606 6 27.665510946651644 38.179076310209496 17.93203843295698 16.22337431018189 7 24.641679578623467 36.073501177875556 22.048973181629645 17.23584606187134 8 24.986797478772452 33.39900089421203 20.970252740204167 20.64394888681135 9 25.533131245773195 17.855619988217615 22.53407049528886 34.07717827072033 10-11 23.86888716003054 27.587567038661774 27.453798439814296 21.089747361493387 12-13 26.239311769094257 26.13666988466771 27.780247575421445 19.84377077081658 14-15 22.61812447130875 27.10237060707285 28.672908234998424 21.606596686619977 16-17 19.643542060148192 30.26184076710737 29.11354060729333 20.98107656545111 18-19 19.380312342686373 28.78328147961433 32.25120929458845 19.585196883110846 20-21 21.209068083265624 26.855390753001622 31.843875317305553 20.0916658464272 22-23 20.46303620941269 27.376108775699514 31.949919767897132 20.210935246990662 24-25 21.034867005028943 27.54957573961359 31.979956865710562 19.435600389646897 26-27 20.404974466632282 27.072198048858443 33.10304874948243 19.419778735026842 28-29 19.50286605488326 28.546248050494288 32.36960376455274 19.581282130069713 30-31 19.958071552943384 27.67179440242156 32.10450004394787 20.265634000687186 32-33 20.055810162636174 27.932612297703596 32.15295166242806 19.858625877232168 34-35 20.961568079319324 27.49438435698345 31.828818056072162 19.71522950762507 36-37 20.411380736966038 27.246245405178023 32.3794412071663 19.962932650689634 38-39 20.05168253382449 27.78150507762369 32.308340001910665 19.858472386641147 40-41 20.003988338649908 27.92262041840506 32.007652670189096 20.065738572755944 42-43 19.832746376558923 28.106367028139058 31.00915158594558 21.051735009356438 44-45 20.15977684270934 27.59679138752331 30.960567917870563 21.28286385189678 46-47 20.908632605414137 28.177533636782314 30.518015891118672 20.395817866684876 48-49 20.67957934987662 28.0562837826549 30.29308507508548 20.971051792383 50-51 19.61676591899325 28.610639345762756 30.79285877209932 20.979735963144673 52-53 20.275440550154688 29.19188221099191 29.509941298721298 21.022735940132105 54-55 19.63372900944407 28.26378310527658 29.90260280351089 22.19988508176846 56-57 20.539164089723393 28.429368509063067 29.29434249265054 21.737124908563008 58-59 21.10095741744272 28.054546643227617 29.392206999753018 21.452288939576643 60-61 20.323110226496784 28.537940753439585 30.163879647251967 20.97506937281167 62-63 19.853359396962585 28.56233805573171 30.03706149287645 21.547241054429257 64-65 19.549283722362784 29.27752607634144 29.484662193427287 21.688528007868484 66-67 19.951722351948124 28.592956446602386 28.832129116194334 22.62319208525516 68-69 19.72348991826009 28.469969072857666 29.214072848584127 22.59246816029812 70-71 20.45620880040883 28.398856172769776 28.79958797230727 22.345347054514125 72-73 20.21405881459883 27.803202165286123 29.61211101595126 22.37062800416379 74-75 19.562686006635037 29.23670177411376 28.654446979619085 22.546165239632117 76-77 19.948817211494205 28.15304228197143 28.946669992081535 22.95147051445283 78-79 19.955389940173543 28.212211340794262 29.036033214474905 22.79636550455729 80-81 20.27075519874244 28.15474880341936 28.684738321307595 22.889757676530603 82-83 19.98734591913156 27.923423197647008 29.09149220088753 22.997738682333903 84-85 19.841555217047997 28.009938756282548 28.779643927820892 23.368862098848567 86-87 19.859901304517685 28.129958509484325 28.576765456726694 23.433374729271293 88-89 19.797672379678033 27.85007408184787 28.168994068350617 24.18325947012348 90-91 19.94899982917383 27.354113905723626 28.496390978484282 24.200495286618263 92-93 20.291385273177777 27.805381398501122 28.407249291930807 23.49598403639029 94-95 20.014339359762843 27.1931720240108 28.61824578200178 24.17424283422458 96-97 19.510239127260682 27.232969474027534 29.037084011619672 24.21970738709211 98-99 19.842492058499424 27.460012828438163 28.07822586913395 24.619269243928464 100-101 19.761685021358417 26.789459863208787 28.776640817187854 24.672214298244945 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5992.0 1 3870.0 2 1903.5 3 2037.0 4 1751.0 5 1679.5 6 2559.5 7 4661.5 8 6531.0 9 6678.5 10 5278.0 11 3953.5 12 3830.0 13 3865.5 14 3872.0 15 3902.5 16 3991.5 17 4143.5 18 4314.0 19 4533.0 20 4792.5 21 5292.5 22 5933.0 23 6703.0 24 7494.5 25 8695.5 26 10464.0 27 12535.0 28 14348.5 29 16297.5 30 18735.0 31 21169.5 32 23971.0 33 26730.0 34 29597.0 35 31842.5 36 34513.5 37 37354.0 38 38216.5 39 38073.0 40 37984.5 41 38929.5 42 40228.5 43 41728.0 44 43452.5 45 44582.5 46 46455.5 47 55829.0 48 72257.0 49 72867.5 50 58770.5 51 47609.5 52 39659.5 53 35918.5 54 33868.5 55 30875.5 56 27586.0 57 24340.0 58 21697.5 59 19141.0 60 16407.5 61 13568.5 62 10973.0 63 8458.0 64 6024.0 65 4454.0 66 3260.5 67 2591.0 68 2015.5 69 1358.0 70 975.5 71 828.0 72 707.5 73 600.0 74 481.5 75 368.5 76 259.5 77 149.0 78 101.5 79 67.0 80 36.0 81 21.5 82 13.0 83 9.5 84 6.0 85 2.5 86 1.0 87 2.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3824554292326702 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.6320553583349494E-5 16-17 0.002251874322167669 18-19 1.4528221433339798E-4 20-21 3.6320553583349494E-5 22-23 0.0 24-25 0.0 26-27 7.264110716669899E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00864429175283718 36-37 0.01579944080875703 38-39 0.010932486628588199 40-41 0.00857165064567048 42-43 0.002833003179501261 44-45 0.0017070660184174264 46-47 9.443343931670869E-4 48-49 0.0 50-51 3.6320553583349494E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.990521788336889E-4 60-61 7.990521788336889E-4 62-63 0.0 64-65 0.0 66-67 7.627316252503395E-4 68-69 6.174494109169415E-4 70-71 0.0017797071255841253 72-73 0.0 74-75 0.0 76-77 0.09908247017537741 78-79 0.03515829586868231 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1525463250500679 88-89 0.3268849822501455 90-91 0.28261022743204245 92-93 0.0 94-95 3.6320553583349494E-5 96-97 0.0 98-99 1.4528221433339798E-4 100-101 5.448083037502424E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1376631.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.87971240929457 #Duplication Level Percentage of deduplicated Percentage of total 1 79.56398201191315 42.86884468940167 2 12.09212310501528 13.030402306320187 3 3.6296382607882793 5.866915969231339 4 1.5249815525574564 3.286622699251011 5 0.7613070942202829 2.0509503645872282 6 0.47023729675501563 1.5201750187970609 7 0.3187024024449132 1.2020115651518226 8 0.23724035191999623 1.0225953546663387 9 0.16958400041705177 0.8223423454519594 >10 1.085580625135267 11.155625515420484 >50 0.09622954481971589 3.5702527700437545 >100 0.04392613111710698 4.225689969743512 >500 0.001751647867796794 0.6379971955084982 >1k 0.003907522166623618 4.553752172083585 >5k 4.042264310300294E-4 1.6092949286341467 >10k+ 4.042264310300294E-4 2.576527135707397 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12990 0.9436079820954199 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11895 0.8640659697478845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10605 0.7703589415028428 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7767 0.564203479363751 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7352 0.534057419889571 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7048 0.5119745233108944 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4360 0.3167152272468076 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4338 0.31511712288914023 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3918 0.2846078578791266 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3504 0.25453443951211324 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3221 0.23397700618393744 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3172 0.2304175919327692 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3127 0.22714874211026775 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2791 0.2027413301022569 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2789 0.20259604788792346 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2477 0.1799320224519134 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2301 0.1671471875905744 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2255 0.16380569666090622 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2089 0.15174727287123418 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2071 0.15043973294223362 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1782 0.1294464529710576 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1730 0.12566911539838926 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1636 0.11884085132471955 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1610 0.11695218253838537 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1510 0.10968807182171547 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 1382 0.10039001010437802 TruSeq Adapter, Index 3 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.6320553583349494E-5 0.0 12-13 7.264110716669899E-5 0.0 0.0 2.1792332150009696E-4 0.0 14-15 7.264110716669899E-5 0.0 0.0 2.9056442866679595E-4 0.0 16-17 7.264110716669899E-5 0.0 0.0 4.7216719658354345E-4 0.0 18-19 7.264110716669899E-5 0.0 0.0 6.53769964500291E-4 0.0 20-21 7.264110716669899E-5 0.0 0.0 0.001234898821833883 0.0 22-23 7.264110716669899E-5 0.0 0.0 0.003523093697584901 0.0 24-25 7.264110716669899E-5 0.0 0.0 0.00799052178833689 0.0 26-27 7.264110716669899E-5 0.0 0.0 0.014709824201256546 0.0 28-29 7.264110716669899E-5 0.0 0.0 0.03544886029734911 0.0 30-31 7.264110716669899E-5 0.0 0.0 0.08949384402937316 0.0 32-33 7.264110716669899E-5 0.0 0.0 0.1914456379378352 0.0 34-35 7.264110716669899E-5 0.0 0.0 0.33716369891423337 0.0 36-37 7.264110716669899E-5 0.0 0.0 0.5433918021604918 0.0 38-39 7.264110716669899E-5 0.0 0.0 0.8205902671086152 0.0 40-41 7.264110716669899E-5 0.0 0.0 1.112280632936495 0.0 42-43 7.264110716669899E-5 0.0 0.0 1.399430929566456 0.0 44-45 7.264110716669899E-5 0.0 0.0 1.7377568861953567 0.0 46-47 7.264110716669899E-5 0.0 0.0 2.1283481194306972 0.0 48-49 7.264110716669899E-5 0.0 0.0 2.50931440596645 0.0 50-51 7.264110716669899E-5 0.0 0.0 2.914906027831714 0.0 52-53 7.264110716669899E-5 0.0 0.0 3.308911393103889 0.0 54-55 7.264110716669899E-5 0.0 0.0 3.7471915131941675 0.0 56-57 7.264110716669899E-5 0.0 0.0 4.271914550812818 0.0 58-59 7.264110716669899E-5 0.0 0.0 4.797363999503135 0.0 60-61 7.264110716669899E-5 0.0 0.0 5.376277303068142 0.0 62-63 7.264110716669899E-5 0.0 0.0 5.9417156812537275 0.0 64-65 7.264110716669899E-5 0.0 0.0 6.521827563086985 0.0 66-67 7.264110716669899E-5 0.0 0.0 7.188927170752366 0.0 68-69 7.264110716669899E-5 0.0 0.0 7.923256123100526 0.0 70-71 7.264110716669899E-5 0.0 0.0 8.710395160358875 0.0 72-73 7.264110716669899E-5 0.0 0.0 9.485112568291722 0.0 74-75 7.264110716669899E-5 0.0 0.0 10.295932606486415 0.0 76-77 7.264110716669899E-5 0.0 0.0 11.110820546682444 0.0 78-79 7.264110716669899E-5 0.0 0.0 12.065942144263786 0.0 80-81 3.6320553583349494E-4 0.0 0.0 13.064176238948564 0.0 82-83 3.6320553583349494E-4 0.0 0.0 14.026779870568074 0.0 84-85 4.7216719658354345E-4 0.0 0.0 15.020728139930018 0.0 86-87 5.08487750166893E-4 0.0 0.0 16.021867878901464 0.0 88-89 5.08487750166893E-4 0.0 0.0 17.06379559954701 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTACGC 80 4.52126E-4 29.656967 3 TTACGCC 95 0.0012340579 24.974289 4 CGTATAG 100 0.0016254226 23.820747 1 TACGCCG 120 1.7230347E-4 23.725573 5 GAGTACT 9995 0.0 23.027828 12-13 GTACATG 10105 0.0 22.34742 1 AGTACTT 10110 0.0 22.08526 12-13 TACATGG 10115 0.0 21.720098 2 TGAACCG 175 4.0864998E-6 21.691954 5 GTACTTT 10820 0.0 21.14043 14-15 ACATGGG 10010 0.0 21.094667 3 GGATATG 465 0.0 20.490963 1 ATGGGAG 1840 0.0 20.11516 5 CATGGGG 2575 0.0 19.164736 4 CATGGGA 6655 0.0 19.1088 4 TTTTACG 150 7.77574E-4 18.980457 2 ATGGGAT 2300 0.0 18.56784 5 CCCTATA 205 1.6039185E-5 18.51752 2 ACTTTTT 12455 0.0 18.440472 16-17 TACTTTT 12035 0.0 18.394993 14-15 >>END_MODULE