##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938794_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1375901 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.491013524955648 33.0 31.0 34.0 30.0 34.0 2 31.526475378679134 33.0 31.0 34.0 28.0 34.0 3 31.51668252294315 33.0 31.0 34.0 28.0 34.0 4 35.09119260760767 37.0 35.0 37.0 32.0 37.0 5 35.133694212010894 37.0 35.0 37.0 33.0 37.0 6 35.224001581509135 37.0 35.0 37.0 33.0 37.0 7 35.141427326530035 37.0 35.0 37.0 32.0 37.0 8 35.125450886364646 37.0 35.0 37.0 32.0 37.0 9 36.65307751066392 39.0 37.0 39.0 32.0 39.0 10-11 36.541983398514866 39.0 37.0 39.0 32.0 39.0 12-13 36.44115637680328 39.0 37.0 39.0 32.0 39.0 14-15 37.5097714152399 40.0 37.0 41.0 32.0 41.0 16-17 37.4202093755292 40.0 37.0 41.0 32.0 41.0 18-19 37.42257328107182 40.0 37.0 41.0 32.0 41.0 20-21 37.34091878703482 40.0 37.0 41.0 32.0 41.0 22-23 37.30659982077199 40.0 37.0 41.0 31.5 41.0 24-25 37.205540950984116 40.0 37.0 41.0 31.0 41.0 26-27 37.04687328521456 40.0 36.0 41.0 31.0 41.0 28-29 36.87086752607927 39.5 36.0 41.0 30.5 41.0 30-31 36.58447700815684 39.0 35.5 41.0 30.0 41.0 32-33 36.3085596274732 39.0 35.0 41.0 30.0 41.0 34-35 35.96119415568417 39.0 35.0 41.0 29.5 41.0 36-37 35.70115764142914 38.5 35.0 40.5 28.0 41.0 38-39 35.48980922319265 38.0 35.0 40.0 27.0 41.0 40-41 35.33082322056602 38.5 35.0 40.0 26.5 41.0 42-43 35.196235048887964 39.0 35.0 41.0 25.5 41.0 44-45 34.89515124998092 39.0 35.0 41.0 24.0 41.0 46-47 34.43824701050439 38.0 34.0 41.0 22.0 41.0 48-49 34.00935786804429 38.0 34.0 40.0 18.0 41.0 50-51 32.82594641620291 37.0 32.0 39.5 14.0 40.5 52-53 32.902554762297584 37.5 33.0 39.5 12.5 40.5 54-55 33.38096236575161 38.0 33.0 40.0 9.5 41.0 56-57 33.25709916629176 38.0 33.0 40.0 8.0 41.0 58-59 33.04772363709307 38.0 33.0 40.0 6.5 41.0 60-61 32.709024123101884 37.0 33.0 40.0 2.0 41.0 62-63 32.320833766382904 37.0 32.0 40.0 2.0 41.0 64-65 31.913414555262335 36.0 31.5 39.5 2.0 41.0 66-67 31.482120079860398 36.0 31.0 39.0 2.0 41.0 68-69 31.021643635697625 35.0 31.0 39.0 2.0 40.0 70-71 30.543116110824833 35.0 30.0 38.0 2.0 40.0 72-73 30.089024573715697 35.0 29.5 37.0 2.0 39.5 74-75 29.599756450500436 35.0 29.0 37.0 2.0 39.0 76-77 29.102028052890432 34.5 29.0 36.0 2.0 39.0 78-79 28.623201087868967 34.0 28.5 36.0 2.0 38.5 80-81 28.090964756912015 34.0 27.0 35.0 2.0 37.0 82-83 27.627264606973903 34.0 26.0 35.0 2.0 37.0 84-85 27.224200360345694 34.0 26.0 35.0 2.0 36.0 86-87 26.771922907244054 34.0 25.0 35.0 2.0 36.0 88-89 26.25817409828178 33.5 23.5 35.0 2.0 35.5 90-91 25.735193157065808 33.0 20.5 35.0 2.0 35.0 92-93 25.211458527902806 33.0 18.0 35.0 2.0 35.0 94-95 24.629642685047834 32.5 5.0 35.0 2.0 35.0 96-97 23.97211863353541 32.0 2.0 35.0 2.0 35.0 98-99 23.198045498913075 32.0 2.0 35.0 2.0 35.0 100-101 20.90922275657914 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16636184308615398 1101 2 0.2641822421096549 1101 3 0.5437784677130715 1101 4 0.19847064999296293 1101 5 0.21359048682801074 1101 6 0.2935124861753593 1101 7 0.2668141076881341 1101 8 0.40148911547039745 1101 9 0.49400658528920616 1101 10-11 0.5133812353748723 1101 12-13 0.479416969548744 1101 14-15 0.6220109949881802 1101 16-17 0.7146063417642026 1101 18-19 0.5504186526914623 1101 20-21 0.7925834905573836 1101 22-23 0.7185237341464443 1101 24-25 0.7847962319406108 1101 26-27 0.6150736186463917 1101 28-29 0.6946271074775581 1101 30-31 0.7666141834903328 1101 32-33 0.6185908678152146 1101 34-35 0.6375796779988079 1101 36-37 0.801452298222884 1101 38-39 0.7587562515123736 1101 40-41 0.7766477534325986 1101 42-43 0.9892430315769545 1101 44-45 1.2271901007475776 1101 46-47 1.0054968265954685 1101 48-49 0.959396869864996 1101 50-51 0.8524577476347517 1101 52-53 0.9796399628301486 1101 54-55 1.0460321159942438 1101 56-57 0.9651961665836097 1101 58-59 0.8709386926726665 1101 60-61 0.745657764505971 1101 62-63 0.7266025124532831 1101 64-65 0.6930099667978418 1101 66-67 0.5831737864110274 1101 68-69 0.5981076606956819 1101 70-71 0.3538894249679707 1101 72-73 0.3685994822025229 1101 74-75 0.35666136751188304 1101 76-77 0.01920657551501037 1101 78-79 0.14175539525786363 1101 80-81 0.08399034206009048 1101 82-83 0.3146843930871448 1101 84-85 0.28422728576554235 1101 86-87 0.18793516051873738 1101 88-89 0.20335802038309225 1101 90-91 0.24582103946613643 1101 92-93 0.2515853328529012 1101 94-95 0.3808418954184951 1101 96-97 0.06379182290364227 1101 98-99 0.3556822512287141 1101 100-101 0.6901359841897197 1102 1 0.2283973502173069 1102 2 0.21159156734050555 1102 3 0.23630060781967543 1102 4 0.3948107054382888 1102 5 0.4689840772008935 1102 6 0.3916168367667936 1102 7 0.45288490994005315 1102 8 0.4707502592664383 1102 9 0.6854876852544081 1102 10-11 0.5950665912181776 1102 12-13 0.5236767306683348 1102 14-15 0.7381281629902787 1102 16-17 0.912563552537101 1102 18-19 0.8595626377032488 1102 20-21 0.9448757952716207 1102 22-23 0.918224845587865 1102 24-25 0.8599616922220719 1102 26-27 0.9275535986436765 1102 28-29 0.9239186471108312 1102 30-31 0.9251100965292025 1102 32-33 0.7891964340096962 1102 34-35 0.9129431490697542 1102 36-37 1.0086619188856005 1102 38-39 0.8918319461344524 1102 40-41 0.9205388584445728 1102 42-43 0.9241688048963752 1102 44-45 0.7964719846903279 1102 46-47 1.0176563137146886 1102 48-49 0.9061828384644244 1102 50-51 1.016557533089454 1102 52-53 0.9511648936387118 1102 54-55 0.7120091727830626 1102 56-57 0.8093564326105565 1102 58-59 0.6687395457840353 1102 60-61 0.6303834530212313 1102 62-63 0.5450521980096141 1102 64-65 0.3714501234089873 1102 66-67 0.3376556745018071 1102 68-69 0.592209105362123 1102 70-71 0.5449819352401057 1102 72-73 0.3360066297179678 1102 74-75 0.5283886299738292 1102 76-77 0.6478090237741085 1102 78-79 0.4102633836710332 1102 80-81 0.3153524396724414 1102 82-83 0.40796291482166325 1102 84-85 0.3862915640890954 1102 86-87 0.40186862151919556 1102 88-89 0.31759734093588676 1102 90-91 0.19406890035045166 1102 92-93 0.16493703273837212 1102 94-95 2.9649869405545815E-4 1102 96-97 -0.03610136534084418 1102 98-99 0.07840463745172599 1102 100-101 0.33663314624631013 1103 1 0.11930065305164916 1103 2 0.24363083091580862 1103 3 0.279546038077914 1103 4 0.2245981809524622 1103 5 0.36950693328665807 1103 6 0.23345372201083592 1103 7 0.2760700080308922 1103 8 0.32242970887728006 1103 9 0.325851902695355 1103 10-11 0.40025219548183344 1103 12-13 0.43841243128641594 1103 14-15 0.4488492663108161 1103 16-17 0.3801213935625185 1103 18-19 0.37814406002614476 1103 20-21 0.48368909240868163 1103 22-23 0.4644397851762534 1103 24-25 0.45028091021912786 1103 26-27 0.5202039353734307 1103 28-29 0.5322067161863941 1103 30-31 0.574709414217331 1103 32-33 0.4488593410759165 1103 34-35 0.32920105660129195 1103 36-37 0.4085094995838432 1103 38-39 0.38395675972439847 1103 40-41 0.4163481788338004 1103 42-43 0.48070893214322297 1103 44-45 0.38309279315953404 1103 46-47 0.5135002490607619 1103 48-49 0.40911035587971156 1103 50-51 0.396632880203704 1103 52-53 0.3060850976513265 1103 54-55 0.2508851740632494 1103 56-57 0.20552234380106427 1103 58-59 0.002578673623311545 1103 60-61 0.08869285852920683 1103 62-63 0.0017020716933942026 1103 64-65 -0.15686604620202615 1103 66-67 -0.2647901275551128 1103 68-69 -0.27926584362337437 1103 70-71 -0.08634465744512809 1103 72-73 0.07936878615033294 1103 74-75 -0.005799598217045343 1103 76-77 0.001428897072017321 1103 78-79 -0.07415562981404022 1103 80-81 0.1663730701867152 1103 82-83 -2.8900715962620893E-4 1103 84-85 0.16434414931565655 1103 86-87 0.024059419161293505 1103 88-89 -0.14345714414109167 1103 90-91 -0.026177841871401597 1103 92-93 0.005099348513031288 1103 94-95 -0.23300206836022497 1103 96-97 -0.38937650771613264 1103 98-99 -0.2598070403862991 1103 100-101 0.38011719272341793 1104 1 0.019494996843320678 1104 2 0.014013502435989977 1104 3 0.02442392862992193 1104 4 0.12120617483105178 1104 5 0.24721007245178583 1104 6 0.19162018121111402 1104 7 0.20253597291593906 1104 8 0.1994952007944093 1104 9 0.21106153615908596 1104 10-11 0.12403477440454225 1104 12-13 0.1930926234677557 1104 14-15 0.20344850501545153 1104 16-17 0.3436551791633349 1104 18-19 0.4525186805567216 1104 20-21 0.36933288047691804 1104 22-23 0.32464283423905016 1104 24-25 0.19097500311046645 1104 26-27 0.1871050529195415 1104 28-29 0.2154393659433964 1104 30-31 0.24821156696989277 1104 32-33 0.248326767004869 1104 34-35 0.40031452731525974 1104 36-37 0.3872714104185846 1104 38-39 0.4935253497754388 1104 40-41 0.5100579273512693 1104 42-43 0.6374426257010484 1104 44-45 0.50343678998793 1104 46-47 0.2640471008853993 1104 48-49 0.19456368148734526 1104 50-51 0.32238662083652514 1104 52-53 0.2677555670983196 1104 54-55 0.16341272458293332 1104 56-57 0.1277224485970052 1104 58-59 0.2430185393292632 1104 60-61 0.11523367765640558 1104 62-63 0.3159357444583719 1104 64-65 0.304886762911341 1104 66-67 0.18567715698249643 1104 68-69 0.30687142796722355 1104 70-71 0.5043739710033641 1104 72-73 0.3432671396342428 1104 74-75 0.48230107242151377 1104 76-77 0.5458806729196013 1104 78-79 0.4662346103017292 1104 80-81 0.3744944782227364 1104 82-83 0.42700534467194373 1104 84-85 0.5405064000441016 1104 86-87 0.5405839089741882 1104 88-89 0.656019153401747 1104 90-91 0.485254896047266 1104 92-93 0.41316447724846483 1104 94-95 0.45969215760532833 1104 96-97 0.5707530732941777 1104 98-99 0.5503126986773523 1104 100-101 0.8802488807420268 1105 1 -0.10801865682838852 1105 2 0.1317650149764944 1105 3 -0.018047084912701905 1105 4 0.18965087378130363 1105 5 0.19515994788962843 1105 6 0.2687192074588154 1105 7 0.20480297745690024 1105 8 0.22224659292036364 1105 9 0.19888896338186868 1105 10-11 0.28745363774473276 1105 12-13 0.32977089452892017 1105 14-15 0.29073754541854413 1105 16-17 0.45276574380051926 1105 18-19 0.29007678057572406 1105 20-21 0.3274850791127548 1105 22-23 0.2499004544729786 1105 24-25 0.3105281848641894 1105 26-27 0.20896537739660914 1105 28-29 0.173294809617488 1105 30-31 0.18072162158458127 1105 32-33 0.18675399246639302 1105 34-35 -0.024520839240004477 1105 36-37 0.25328453828010566 1105 38-39 0.2860096089182491 1105 40-41 0.1741099036215772 1105 42-43 -0.07784921902305797 1105 44-45 -0.13022683598843798 1105 46-47 0.06751182878866047 1105 48-49 0.09082066218623908 1105 50-51 0.17857097818409073 1105 52-53 0.11582184326507416 1105 54-55 -0.13022457074578142 1105 56-57 -0.25520116309016316 1105 58-59 -0.02962434591032803 1105 60-61 0.015397441440526904 1105 62-63 0.008644242246759859 1105 64-65 0.09900785713170634 1105 66-67 0.14947117853402148 1105 68-69 0.11154779380267144 1105 70-71 -0.09334430712213049 1105 72-73 -0.005718568496948961 1105 74-75 0.06073616496678369 1105 76-77 0.053614765488955385 1105 78-79 -0.17027053139943504 1105 80-81 -0.14000182772654668 1105 82-83 -0.19248067252325285 1105 84-85 -0.21861473431444978 1105 86-87 -0.02250956750082267 1105 88-89 -0.07124484077554172 1105 90-91 0.16017655969464784 1105 92-93 -0.028724800237494463 1105 94-95 0.17504822342123916 1105 96-97 -0.17693911501276816 1105 98-99 0.0932926643725871 1105 100-101 0.22954204735026806 1106 1 0.2251020846266698 1106 2 0.10469583420645279 1106 3 0.23148518740318735 1106 4 0.22834310666548419 1106 5 0.30142266954604224 1106 6 0.12151364082224347 1106 7 0.13652930016422005 1106 8 0.19667475398528467 1106 9 0.15743508023279418 1106 10-11 0.26517569532749263 1106 12-13 0.20245007856450314 1106 14-15 0.492143053694555 1106 16-17 0.43942866966779803 1106 18-19 0.37345853477556545 1106 20-21 0.39572119510001613 1106 22-23 0.31217914300594884 1106 24-25 0.24040199420912955 1106 26-27 0.21685912847298283 1106 28-29 0.18209693103912628 1106 30-31 0.1786675036645704 1106 32-33 0.3297759451330151 1106 34-35 0.36771322032967646 1106 36-37 0.5692908669767789 1106 38-39 0.4390706508944149 1106 40-41 0.7149725156092046 1106 42-43 0.6381239432964705 1106 44-45 0.5359533223414132 1106 46-47 0.6667539080803238 1106 48-49 0.6538089272167653 1106 50-51 0.6250471400421347 1106 52-53 0.48830223345293433 1106 54-55 0.4366317421853765 1106 56-57 0.7247594971915916 1106 58-59 0.6454624411467123 1106 60-61 0.7087517988841299 1106 62-63 0.7729383631103559 1106 64-65 0.7257803291408713 1106 66-67 0.6674896178132279 1106 68-69 1.093502780538678 1106 70-71 0.762178047987053 1106 72-73 0.8082635380126035 1106 74-75 0.6445740207323958 1106 76-77 0.6947701512435636 1106 78-79 0.7114954223178849 1106 80-81 0.6906976148841224 1106 82-83 0.8278346278062152 1106 84-85 0.8014591004801979 1106 86-87 0.9931215569722305 1106 88-89 1.0741125563230902 1106 90-91 1.0550777274679675 1106 92-93 0.9526470919477639 1106 94-95 1.158261130610068 1106 96-97 1.1862005195233962 1106 98-99 1.476000972473411 1106 100-101 1.6753868215506103 1107 1 0.18649201713199304 1107 2 0.1881701866779899 1107 3 0.31756745879133774 1107 4 0.4336131078175782 1107 5 0.19510246857319657 1107 6 0.14009076145831756 1107 7 0.16541989474878704 1107 8 0.17483436819882314 1107 9 0.21201734189548205 1107 10-11 0.15840513084695118 1107 12-13 0.11756777904732729 1107 14-15 0.3182324171857829 1107 16-17 0.386674794072853 1107 18-19 0.3430462801002392 1107 20-21 0.3537610814468266 1107 22-23 0.2506558969174506 1107 24-25 0.16747602289773766 1107 26-27 0.22421381891166448 1107 28-29 0.2837100104332819 1107 30-31 0.36999793133035297 1107 32-33 0.530477225452131 1107 34-35 0.5320914119630871 1107 36-37 0.5203942575706222 1107 38-39 0.527775055329819 1107 40-41 0.536662231610876 1107 42-43 0.5237226349979593 1107 44-45 0.7807520726948951 1107 46-47 0.801164223648982 1107 48-49 0.6992058680366071 1107 50-51 0.7194536552011357 1107 52-53 0.49640728036704473 1107 54-55 0.6500045459151593 1107 56-57 0.7623356737101474 1107 58-59 0.6686636429140478 1107 60-61 0.6341620261755594 1107 62-63 0.6619551791750098 1107 64-65 0.6504102754713053 1107 66-67 0.44774289865901906 1107 68-69 0.6613071761463587 1107 70-71 0.9461732361297734 1107 72-73 0.7983398304885405 1107 74-75 0.8512676792850371 1107 76-77 0.9395344884386496 1107 78-79 0.8219212627096546 1107 80-81 0.8914370489223273 1107 82-83 0.9027445925102846 1107 84-85 0.9474412146151785 1107 86-87 0.8241312540017773 1107 88-89 1.0398647307110238 1107 90-91 1.1193839507136047 1107 92-93 1.1710322470089665 1107 94-95 1.1521198780608763 1107 96-97 1.1473360939014547 1107 98-99 1.3335641617652314 1107 100-101 1.6791745133104534 1108 1 0.1253662872164547 1108 2 0.05111903179244237 1108 3 -0.03741482103170668 1108 4 0.04169798978780648 1108 5 0.15257131139235725 1108 6 0.17748976191464294 1108 7 0.21966686237154676 1108 8 0.22927479588765465 1108 9 0.07705763241348507 1108 10-11 0.3760528969023724 1108 12-13 0.44100896363501363 1108 14-15 0.5133489167608829 1108 16-17 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92-93 -2.199717921320353 2316 94-95 -2.243590540470745 2316 96-97 -2.3788254644110047 2316 98-99 -2.6879065334542105 2316 100-101 -2.6364201772000726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9101.0 3 1780.0 4 1595.0 5 1725.0 6 2020.0 7 2437.0 8 3202.0 9 3674.0 10 4569.0 11 6061.0 12 8234.0 13 10378.0 14 11572.0 15 13496.0 16 16785.0 17 21130.0 18 18136.0 19 12268.0 20 12209.0 21 12547.0 22 13641.0 23 14810.0 24 16372.0 25 18493.0 26 21076.0 27 23925.0 28 27716.0 29 32313.0 30 37804.0 31 44530.0 32 53790.0 33 65994.0 34 85503.0 35 121243.0 36 161991.0 37 227520.0 38 204269.0 39 31992.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51097615280396 20.011354766487553 15.146587994703054 20.331081086005433 2 15.215656696554678 22.949579636160315 39.25007207485301 22.584691592431994 3 19.65784889479265 27.480089372065112 31.622723459293177 21.239338273849057 4 12.257775182753832 18.116963909348566 38.79229947676455 30.832961431133054 5 12.078960923901679 39.08352986862918 34.155976530880636 14.681532676588512 6 27.745566240950065 37.50537061230077 18.48721320930314 16.261849937446023 7 24.829258910083887 35.766014399517395 22.46664228375588 16.938084406642837 8 24.94318541991057 32.70210070042778 21.44213947788148 20.91257440178018 9 26.070515858024944 17.705101077485338 22.849269241771463 33.37511382271825 10-11 23.861574709671782 27.287866483614525 27.73269046587255 21.11786834084114 12-13 26.35355856528437 25.985083269298702 27.958418156458148 19.702940008958777 14-15 22.822291533795216 26.72128593353661 28.999721880032737 21.45670065263544 16-17 19.665220882129017 30.075270451363945 29.24034537011829 21.019163296388747 18-19 19.423073630745122 28.621644136703107 32.452909638157045 19.502372594394725 20-21 21.332285616075225 26.785792313115014 31.915917048758182 19.966005022051583 22-23 20.56521799319051 27.092008215682807 32.07675499472858 20.266018796398097 24-25 20.970433349640054 27.269005193988683 32.017394161416156 19.743167294955104 26-27 20.347810167869625 26.808121283907973 33.25267821195801 19.591390336264393 28-29 19.565585923814393 28.373847367022254 32.41809301560833 19.642473693555026 30-31 20.04499119420567 27.415834684332687 32.100218395362674 20.438955726098975 32-33 19.963662446704316 27.684190979693934 32.20271839852611 20.14942817507564 34-35 20.880505622014063 27.348420441817474 31.79438699458132 19.97668694158715 36-37 20.580528075202885 27.021840039171817 32.37527300961125 20.02235887601405 38-39 20.15250016142312 27.76827884284611 32.17058830610785 19.90863268962292 40-41 20.1092588905493 27.91414779013483 31.920574526290764 20.05601879302511 42-43 19.79352666968462 28.07153792755523 30.925037143823292 21.20989825893686 44-45 20.228686542640048 27.522315598081377 30.944624337682384 21.304373521596187 46-47 20.895101466163535 28.155214061627248 30.59238669037123 20.35729778183799 48-49 20.65695937072259 28.023075353970366 30.371371030570938 20.948594244736103 50-51 19.71976935575266 28.52958271897648 30.87316938078303 20.87747854448783 52-53 20.376763123932513 29.013803604312976 29.55163006296656 21.057803208787952 54-55 19.714399236140732 28.10316916221027 30.12672489500401 22.055706706644983 56-57 20.58891227926824 28.22510307715822 29.5179859290514 21.667998714522145 58-59 21.25591426710497 27.738942955357544 29.597957476355695 21.407185301181798 60-61 20.414306562211422 27.976046603108358 30.697927409949642 20.911719424730578 62-63 20.013526868234514 28.02287018401721 30.596623171291814 21.366979776456464 64-65 19.801128909296402 28.648107943785906 30.019301743287908 21.531461403629777 66-67 20.085947964073387 28.042174720714737 29.343109382014067 22.528767933197813 68-69 19.762687021474303 27.874329452693246 29.852229585762537 22.510753940069915 70-71 20.395848806632273 27.83763118468139 29.45394690020019 22.31257310848615 72-73 20.187287308292355 27.117647595500287 30.223617104746957 22.4714479914604 74-75 19.551956454625273 28.513338252477638 29.40874658889456 22.525958704002534 76-77 19.813019458805062 27.47890786083767 29.565846551609017 23.14222612874825 78-79 19.87248326064658 27.453378584424847 29.684574990406514 22.989563164522057 80-81 20.18658680686153 27.405087742787025 29.33425071421093 23.074074736140517 82-83 19.866919285065123 27.05683877480806 29.83969093284561 23.236551007281204 84-85 19.683990503734766 27.122914026838952 29.632959205973876 23.560136263452407 86-87 19.776694555525193 27.116938021502378 29.430291656436214 23.676075766536215 88-89 19.743536417172006 26.80535183928428 29.059268664667208 24.39184307887651 90-91 19.887678255754036 26.28857250879874 29.342404323244885 24.481344912202335 92-93 20.367041983054378 26.469457994866758 29.327740507273802 23.835759514805066 94-95 19.972218379884822 25.951799692037163 29.470817072338416 24.605164855739595 96-97 19.55894461460301 25.74998321123767 29.84308346788675 24.847988706272574 98-99 20.035693560925406 25.961737086631704 28.981529129668353 25.021040222774538 100-101 20.064646514769127 24.872343383255174 29.624909377028413 25.438100724947287 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6898.0 1 4475.0 2 2254.5 3 2308.5 4 1933.0 5 1905.0 6 2812.5 7 4740.0 8 6686.5 9 7013.5 10 5472.5 11 4158.0 12 3993.5 13 3994.5 14 4046.5 15 4135.0 16 4144.0 17 4216.0 18 4360.5 19 4602.0 20 4982.0 21 5446.5 22 6092.0 23 6929.0 24 7846.0 25 8966.5 26 10691.0 27 12689.5 28 14397.5 29 16343.5 30 18668.5 31 21025.5 32 23796.0 33 26584.0 34 29242.0 35 31388.0 36 34142.5 37 37225.0 38 37993.5 39 37508.0 40 37410.0 41 38375.0 42 39376.5 43 40566.5 44 42050.0 45 43369.5 46 46036.5 47 54051.0 48 69437.5 49 70204.5 50 56022.0 51 46038.0 52 40060.5 53 37338.0 54 34887.0 55 31965.5 56 28672.0 57 25115.0 58 22383.5 59 19880.0 60 17007.5 61 14108.0 62 11472.0 63 8867.0 64 6565.5 65 4980.0 66 3782.5 67 3060.0 68 2387.0 69 1684.0 70 1236.5 71 1000.0 72 858.5 73 736.0 74 590.5 75 443.5 76 345.5 77 245.0 78 159.5 79 117.5 80 87.5 81 63.0 82 49.0 83 35.0 84 31.0 85 23.5 86 15.0 87 14.0 88 10.0 89 7.0 90 3.5 91 1.5 92 1.5 93 4.0 94 5.5 95 2.5 96 1.5 97 1.5 98 2.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27545586492051394 2 0.16927089957780392 3 0.2009592259908235 4 0.16948893852101277 5 0.24522113146222005 6 0.19529021346739336 7 0.24384021815523063 8 0.2510355032811227 9 0.149283996450326 10-11 0.20975346336691375 12-13 0.21411424223109077 14-15 0.17392239703292606 16-17 0.2893740174620122 18-19 0.23602715602358018 20-21 0.2003777888089332 22-23 0.24740152089430856 24-25 0.23668127285320673 26-27 0.35155145610040256 28-29 0.32135306246597684 30-31 0.2963149238208272 32-33 0.19376394086493143 34-35 0.36307118026660345 36-37 0.40177309268617434 38-39 0.38371220022370794 40-41 0.34551904533829103 42-43 0.3790607027685858 44-45 0.3877459206730717 46-47 0.3938873509067876 48-49 0.41387425403426553 50-51 0.40449857947628504 52-53 0.4048619777149664 54-55 0.3991202855438 56-57 0.3782612266434867 58-59 0.3526416508164468 60-61 0.32011750845446 62-63 0.3848750745874885 64-65 0.42281385070582833 66-67 0.4277924065757638 68-69 0.4315717482580505 70-71 0.43124468984323727 72-73 0.42252313211488324 74-75 0.41747189659721157 76-77 0.402972306873823 78-79 0.37640789562621146 80-81 0.387564221553731 82-83 0.3964311385775575 84-85 0.3973759739981292 86-87 0.3925791172475345 88-89 0.40199113162938327 90-91 0.42292287017743285 92-93 0.42245045246714696 94-95 0.4310266509000284 96-97 0.43149906861031423 98-99 0.42924599953048953 100-101 0.40159139356683365 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1375901.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.758862207073555 #Duplication Level Percentage of deduplicated Percentage of total 1 80.54657983818805 44.91185646448576 2 11.66195247666932 13.005144024240897 3 3.3709507744450113 5.638811392173217 4 1.407959729732791 3.140249302531169 5 0.7075005406553962 1.9724712578917134 6 0.45722714737952685 1.529667930484101 7 0.3127450228163772 1.2206814643216517 8 0.2173224328651821 0.9694121270908546 9 0.16614349721105165 0.8337575130833103 >10 1.0206247503764165 10.848821471373652 >50 0.08515411591376987 3.249897888481806 >100 0.0402079718668015 3.9184389060706355 >500 0.0014406679228694084 0.5462440285424982 >1k 0.0034052150904186015 4.110924478441557 >5k 3.929094335098387E-4 1.5760746823535021 >10k+ 3.929094335098387E-4 2.5275470684337438 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13180 0.9579177571642147 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11181 0.8126311413393842 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10250 0.7449663892969044 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7498 0.5449519987266526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7211 0.5240929398263393 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6873 0.4995272188914755 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4563 0.3316372326206609 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4416 0.3209533244034273 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3953 0.28730264750152806 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3234 0.23504598077914038 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3144 0.22850481248287485 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2726 0.19812471972910842 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2717 0.19747060289948187 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2673 0.1942726983990854 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2530 0.18387950877279688 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2203 0.16011326396303222 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2185 0.15880503030377913 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2065 0.15008347257542512 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1945 0.14136191484707114 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1939 0.14092583696065342 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1933 0.14048975907423572 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1583 0.11505188236653655 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1526 0.11090914244556839 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1419 0.10313242013778606 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1379 0.10022523422833474 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.267964773628335E-5 0.0 16-17 0.0 0.0 0.0 2.907185909451334E-4 0.0 18-19 3.6339823868141676E-5 0.0 0.0 5.450973580221252E-4 0.0 20-21 7.267964773628335E-5 0.0 0.0 0.0013082336592531003 0.0 22-23 7.267964773628335E-5 0.0 0.0 0.003270584148132751 0.0 24-25 7.267964773628335E-5 0.0 0.0 0.006577508120133644 0.0 26-27 7.267964773628335E-5 0.0 0.0 0.013118676416399145 0.0 28-29 7.267964773628335E-5 0.0 0.0 0.034268453907657606 0.0 30-31 7.267964773628335E-5 0.0 0.0 0.08140120546463736 0.0 32-33 7.267964773628335E-5 0.0 0.0 0.17134226953828802 0.0 34-35 1.816991193407084E-4 0.0 0.0 0.30594497714588476 0.0 36-37 2.907185909451334E-4 0.0 0.0 0.48731703807177984 0.0 38-39 3.633982386814168E-4 0.0 0.0 0.7383162015290343 0.0 40-41 3.633982386814168E-4 0.0 0.0 1.007012859210074 0.0 42-43 3.633982386814168E-4 0.0 0.0 1.2742922637602558 0.0 44-45 3.633982386814168E-4 0.0 0.0 1.5894675561686489 0.0 46-47 3.9973806254955843E-4 0.0 0.0 1.966638588096091 0.0 48-49 5.087575341539835E-4 0.0 0.0 2.332907672863091 0.0 50-51 5.087575341539835E-4 0.0 0.0 2.728975413201967 0.0 52-53 5.087575341539835E-4 0.0 0.0 3.1078180770273445 0.0 54-55 5.450973580221252E-4 0.0 0.0 3.5239090603175667 0.0 56-57 5.814371818902668E-4 0.0 0.0 4.045494552297004 0.0 58-59 5.814371818902668E-4 0.0 0.0 4.55010934653002 0.0 60-61 5.814371818902668E-4 0.0 0.0 5.122425232629382 0.0 62-63 5.814371818902668E-4 0.0 0.0 5.681295383897533 0.0 64-65 5.814371818902668E-4 7.267964773628335E-5 0.0 6.2498319283146095 0.0 66-67 5.814371818902668E-4 7.267964773628335E-5 0.0 6.904166796884369 0.0 68-69 5.814371818902668E-4 7.267964773628335E-5 0.0 7.637359083247995 0.0 70-71 6.177770057584084E-4 1.0901947160442502E-4 0.0 8.415249352969436 0.0 72-73 6.541168296265501E-4 1.453592954725667E-4 0.0 9.168101484045728 0.0 74-75 6.541168296265501E-4 1.453592954725667E-4 0.0 9.962926111689722 0.0 76-77 6.541168296265501E-4 1.453592954725667E-4 0.0 10.768361967903214 0.0 78-79 6.541168296265501E-4 1.453592954725667E-4 0.0 11.721228489549757 0.0 80-81 7.267964773628335E-4 1.453592954725667E-4 0.0 12.706619153558286 0.0 82-83 7.267964773628335E-4 1.453592954725667E-4 0.0 13.650691437828739 0.0 84-85 7.631363012309753E-4 1.453592954725667E-4 0.0 14.634047071700653 0.0 86-87 7.994761250991169E-4 1.816991193407084E-4 0.0 15.615294995788215 0.0 88-89 7.994761250991169E-4 2.1803894320885004E-4 0.0 16.662499700196452 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATGCG 65 0.0058834166 29.191055 5 GAGTACT 9370 0.0 23.475302 12-13 GTACATG 9550 0.0 23.142387 1 TACATGG 9630 0.0 22.412394 2 AGTACTT 9510 0.0 22.331278 12-13 CATGGGG 2620 0.0 22.269321 4 GTACTTT 10270 0.0 21.37147 14-15 ACATGGG 9635 0.0 21.12072 3 GTACACG 160 4.966785E-5 20.793934 1 GGTAAGG 260 1.5141268E-8 20.06885 3 ACTTTTT 11720 0.0 19.153948 16-17 AGAGTAC 16550 0.0 18.667408 10-11 TACTTTT 11370 0.0 18.594322 14-15 CATGGGA 6455 0.0 18.371597 4 ATGGGAG 2060 0.0 18.19127 5 ATGGGGA 1465 0.0 17.808537 5 GTAAGGT 275 5.9931153E-7 17.24926 4 TAAGGTG 370 2.7957867E-9 16.666515 5 GTATCAA 26420 0.0 16.568584 1 TAGGACA 580 0.0 16.357058 4 >>END_MODULE