##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938794_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1375901 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.992510362300777 31.0 31.0 34.0 30.0 34.0 2 32.17128994019192 33.0 31.0 34.0 30.0 34.0 3 32.23103188383467 34.0 31.0 34.0 30.0 34.0 4 35.88161648258123 37.0 35.0 37.0 35.0 37.0 5 35.79524471600791 37.0 35.0 37.0 35.0 37.0 6 35.84001247182755 37.0 35.0 37.0 35.0 37.0 7 35.76130186692211 37.0 35.0 37.0 33.0 37.0 8 35.763039637299485 37.0 35.0 37.0 33.0 37.0 9 37.369833294691986 39.0 37.0 39.0 34.0 39.0 10-11 37.30359778792224 39.0 37.0 39.0 34.0 39.0 12-13 37.23373047915511 39.0 37.0 39.0 33.0 39.0 14-15 38.35797524676558 40.0 38.0 41.0 33.0 41.0 16-17 38.307490873253236 40.0 38.0 41.0 33.0 41.0 18-19 38.47576424466586 40.0 38.0 41.0 34.0 41.0 20-21 38.484432019454886 40.0 38.0 41.0 34.0 41.0 22-23 38.38996446692022 40.0 38.0 41.0 34.0 41.0 24-25 38.28311084881834 40.0 38.0 41.0 33.5 41.0 26-27 38.06650151428046 40.0 37.0 41.0 33.0 41.0 28-29 37.87295197837635 40.0 37.0 41.0 33.0 41.0 30-31 37.58829305306123 40.0 36.5 41.0 32.5 41.0 32-33 37.317539197951014 39.5 36.0 41.0 32.0 41.0 34-35 37.180878202719526 40.0 35.0 41.0 31.5 41.0 36-37 36.99171924433517 39.5 35.0 41.0 31.0 41.0 38-39 36.81139268014196 39.0 35.0 41.0 31.0 41.0 40-41 36.60561115952383 39.0 35.0 41.0 30.5 41.0 42-43 36.319029130729604 39.0 35.0 41.0 30.0 41.0 44-45 35.95210629253123 39.0 35.0 41.0 29.5 41.0 46-47 35.48513773883441 39.0 35.0 41.0 27.0 41.0 48-49 35.04200483901094 39.0 35.0 41.0 24.5 41.0 50-51 34.73719875194509 39.0 35.0 40.0 24.0 41.0 52-53 34.518626340121855 38.0 34.0 40.0 23.0 41.0 54-55 34.24819118526696 38.0 34.0 40.0 22.5 41.0 56-57 33.944614111044324 38.0 34.0 40.0 21.5 41.0 58-59 33.614671404410636 38.0 33.0 40.0 19.5 41.0 60-61 33.20109731732152 37.0 33.0 40.0 18.0 41.0 62-63 32.84464434577779 36.5 32.5 40.0 14.0 41.0 64-65 32.43448947271642 36.0 32.0 39.0 10.0 41.0 66-67 31.982247632642174 35.5 31.5 39.0 7.5 40.0 68-69 31.6407365064783 35.0 31.0 38.5 4.0 40.0 70-71 31.196784870423087 35.0 31.0 38.0 2.0 40.0 72-73 30.711724535413524 35.0 30.5 37.0 2.0 39.5 74-75 30.238091258019292 35.0 30.0 37.0 2.0 39.0 76-77 28.310821054712513 32.5 27.0 35.0 2.0 37.0 78-79 29.215295286506805 34.0 29.0 35.0 2.0 37.5 80-81 29.23612163956564 34.0 29.0 35.0 2.0 37.0 82-83 28.913195789522646 34.0 29.0 35.0 2.0 37.0 84-85 28.522310108067366 34.0 29.0 35.0 2.0 36.0 86-87 27.981483406146225 34.0 28.0 35.0 2.0 36.0 88-89 27.393885897313833 33.5 26.5 35.0 2.0 35.0 90-91 26.874759157817316 33.0 25.5 35.0 2.0 35.0 92-93 26.595028276016954 33.0 25.0 35.0 2.0 35.0 94-95 26.262829229719287 33.0 25.0 35.0 2.0 35.0 96-97 25.677431370425637 33.0 23.5 35.0 2.0 35.0 98-99 24.93724330456915 32.0 10.5 34.5 2.0 35.0 100-101 21.653420558601237 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11225601091744863 1101 2 0.1280606577522292 1101 3 0.06391177653444657 1101 4 0.15200582636687443 1101 5 -0.0951989076602544 1101 6 0.0861353553284232 1101 7 0.1688094630642638 1101 8 0.3155859603772271 1101 9 0.3570972898424216 1101 10-11 0.22799891293951902 1101 12-13 0.1931341570807703 1101 14-15 0.38316424797095294 1101 16-17 0.32836921333774427 1101 18-19 0.38563695918082175 1101 20-21 0.41174246853129404 1101 22-23 0.28193149619532676 1101 24-25 0.34631492329527447 1101 26-27 0.2941620650173533 1101 28-29 0.35685113217008535 1101 30-31 0.34160482390134916 1101 32-33 0.38740092781893765 1101 34-35 0.47826348426798404 1101 36-37 0.39475715507712295 1101 38-39 0.530531860621636 1101 40-41 0.6048439545234601 1101 42-43 0.5054334899345392 1101 44-45 0.33768058410186796 1101 46-47 0.2506174331509996 1101 48-49 0.14972000747820857 1101 50-51 0.2216493715511234 1101 52-53 0.04511577877311623 1101 54-55 0.1507426546792061 1101 56-57 0.17648230639016305 1101 58-59 0.17713738223004327 1101 60-61 0.18606550266152766 1101 62-63 0.13022789155336767 1101 64-65 0.052828643180305335 1101 66-67 0.03871768310909829 1101 68-69 0.10502981323309868 1101 70-71 0.04504576371734004 1101 72-73 -0.13221283487768787 1101 74-75 -0.16270636006364825 1101 76-77 -0.19798236477068087 1101 78-79 -0.3630161229044866 1101 80-81 -0.056749683733091416 1101 82-83 0.003415041981813971 1101 84-85 0.09987602161489306 1101 86-87 0.07567861343769167 1101 88-89 0.25827707204252803 1101 90-91 0.06954285806292049 1101 92-93 0.11992336680485138 1101 94-95 0.2522337507991672 1101 96-97 5.175087007174284E-4 1101 98-99 -0.3198715080129553 1101 100-101 0.2133013496435865 1102 1 0.33836163357122473 1102 2 0.2835927462158665 1102 3 0.24131723027124252 1102 4 0.1817155104022632 1102 5 -0.0160988687148631 1102 6 0.14711253886259357 1102 7 0.19953022925137276 1102 8 0.24439310236196832 1102 9 0.3074315518734352 1102 10-11 0.30229299900190654 1102 12-13 0.34656284899971723 1102 14-15 0.38620008694444863 1102 16-17 0.37725834803578095 1102 18-19 0.32810955338140957 1102 20-21 0.2991721536728207 1102 22-23 0.3156993356020692 1102 24-25 0.30682049893804475 1102 26-27 0.2534825717221807 1102 28-29 0.3023952844857192 1102 30-31 0.3686796451328078 1102 32-33 0.44129563931198135 1102 34-35 0.40877045410950785 1102 36-37 0.19616650565703964 1102 38-39 0.3930788428150649 1102 40-41 0.3597605215221975 1102 42-43 0.43147326262253927 1102 44-45 0.46939491853904514 1102 46-47 0.5403183948024619 1102 48-49 0.39187216641225575 1102 50-51 0.4105272950476291 1102 52-53 0.3670086167395681 1102 54-55 0.2847023980544563 1102 56-57 0.21200470459637444 1102 58-59 0.2926836200826557 1102 60-61 0.5744858178656642 1102 62-63 0.5358801000333244 1102 64-65 0.4931079668671643 1102 66-67 0.36818269102491996 1102 68-69 0.2219230083909629 1102 70-71 0.10420443966637549 1102 72-73 0.1888610553516301 1102 74-75 0.3097726174932127 1102 76-77 0.17036004813798655 1102 78-79 0.13259618383767702 1102 80-81 0.12938074644059583 1102 82-83 0.2049991859092124 1102 84-85 0.5432983416998809 1102 86-87 0.5258210291117145 1102 88-89 0.3184318511484463 1102 90-91 0.5630261311346061 1102 92-93 0.8681028428165654 1102 94-95 0.7228219183150308 1102 96-97 0.7144025760255346 1102 98-99 0.8682375897676167 1102 100-101 1.4212979436506004 1103 1 0.15318977314780824 1103 2 0.1301117299661314 1103 3 0.06741963152781949 1103 4 0.025017005541414505 1103 5 0.04434735780087351 1103 6 0.04265594828830643 1103 7 0.07492319413024262 1103 8 0.1225450495384095 1103 9 0.0325206995737517 1103 10-11 0.19420751254260438 1103 12-13 0.09620251297359772 1103 14-15 0.1638894988533579 1103 16-17 0.1187572551110847 1103 18-19 0.046081638972282235 1103 20-21 0.18858105505083955 1103 22-23 0.11078616807248665 1103 24-25 0.21970032715707077 1103 26-27 0.15160337678663183 1103 28-29 0.07883628811519117 1103 30-31 0.2037640602308315 1103 32-33 0.3142709480660457 1103 34-35 0.23344030686542538 1103 36-37 0.3820899646105147 1103 38-39 0.08862838079051016 1103 40-41 0.26121514415106617 1103 42-43 0.1642806945105164 1103 44-45 0.07335044342662655 1103 46-47 -0.08844044903528214 1103 48-49 -0.17706210148708124 1103 50-51 -0.16969967692674004 1103 52-53 -0.2569288175771334 1103 54-55 -0.2396951882048839 1103 56-57 -0.2138469368335265 1103 58-59 0.03405030937199882 1103 60-61 -0.0023775449830623074 1103 62-63 0.003525613367457936 1103 64-65 0.03188409548499749 1103 66-67 -0.21688923999030862 1103 68-69 -0.29259881719050895 1103 70-71 -0.15341567423385527 1103 72-73 -0.1710190180093818 1103 74-75 -0.04040136522428028 1103 76-77 -0.06822410933396483 1103 78-79 -0.2592587465805849 1103 80-81 0.13938588761287463 1103 82-83 0.11940349087492663 1103 84-85 0.27122336229862043 1103 86-87 0.4366432535028899 1103 88-89 0.08500645716214095 1103 90-91 0.12068642108310357 1103 92-93 0.15825732766656486 1103 94-95 0.27989800993458047 1103 96-97 0.46195058867304795 1103 98-99 0.44159349280942095 1103 100-101 1.244755421729689 1104 1 0.10226504601924091 1104 2 0.09574032539558885 1104 3 0.06986875143600457 1104 4 0.06990349046937183 1104 5 -0.08662372351024317 1104 6 0.003835429354339226 1104 7 0.15278158653836016 1104 8 0.003953196452336272 1104 9 0.17183886059909526 1104 10-11 0.03157974050981238 1104 12-13 -0.08571103869384444 1104 14-15 0.08000533995166137 1104 16-17 0.07694062582532268 1104 18-19 0.004835565354525784 1104 20-21 0.05730612096415655 1104 22-23 0.16241286164972024 1104 24-25 0.2095969451315156 1104 26-27 0.10660461049544523 1104 28-29 -0.05952013451740612 1104 30-31 -0.16380131940493925 1104 32-33 -0.00642874503156321 1104 34-35 -0.09811380582070939 1104 36-37 0.02214308339865312 1104 38-39 0.11364494575705919 1104 40-41 0.08357909656595552 1104 42-43 0.151438201697907 1104 44-45 0.24733998046699668 1104 46-47 0.1745072555732463 1104 48-49 0.10855146764635748 1104 50-51 -0.02945637291593073 1104 52-53 0.03556080679319251 1104 54-55 0.027436314064310352 1104 56-57 -0.03839746483317441 1104 58-59 -0.002410637506443436 1104 60-61 0.02045743468153205 1104 62-63 -0.14756517928351798 1104 64-65 -0.4987800834332745 1104 66-67 -0.31564998642572206 1104 68-69 -0.2696094159967224 1104 70-71 -0.279260348909002 1104 72-73 -0.3777717023656777 1104 74-75 -0.17958201623439507 1104 76-77 -0.06487673851991005 1104 78-79 -0.22004759939773777 1104 80-81 -0.2037429088140854 1104 82-83 -0.10356844432902435 1104 84-85 -0.00120199831862422 1104 86-87 0.12628869390920627 1104 88-89 0.16604754105156871 1104 90-91 0.12213221690845799 1104 92-93 0.4466019676869699 1104 94-95 0.575485768216371 1104 96-97 0.35030964506601237 1104 98-99 0.7247202376756654 1104 100-101 1.2206842381177765 1105 1 0.07631811939987188 1105 2 0.03428395158601916 1105 3 -0.005359835512670941 1105 4 0.013155057865397168 1105 5 -0.21086049597223422 1105 6 -0.07206747905835442 1105 7 0.013281601255691555 1105 8 0.0033398850255323964 1105 9 0.03727212551912373 1105 10-11 0.12080558200680258 1105 12-13 -0.020538404371414742 1105 14-15 0.06684376236054135 1105 16-17 0.05325829545597571 1105 18-19 0.0904784051041645 1105 20-21 -0.1042072490643946 1105 22-23 -0.0528045936430388 1105 24-25 -0.07499159836088864 1105 26-27 -0.03690036262535301 1105 28-29 0.00672178098368903 1105 30-31 0.006808165515145959 1105 32-33 0.17533246489380616 1105 34-35 -0.027264294569967262 1105 36-37 0.005762155992570683 1105 38-39 0.06346151426357238 1105 40-41 -0.28403046060286385 1105 42-43 -0.10606242044391934 1105 44-45 0.029486516943244112 1105 46-47 0.055931037178723386 1105 48-49 0.20547072835455538 1105 50-51 0.11206925263763878 1105 52-53 0.19169111138847938 1105 54-55 0.3218902876105787 1105 56-57 0.22231554983866175 1105 58-59 0.08966367700029565 1105 60-61 0.14039460504670842 1105 62-63 0.19173732614182626 1105 64-65 0.0756840049349421 1105 66-67 0.29751132357751686 1105 68-69 -0.016093177426068195 1105 70-71 0.006091890400192312 1105 72-73 0.2181762143288637 1105 74-75 0.07658227202197665 1105 76-77 0.07287188407146417 1105 78-79 -0.24819115494496913 1105 80-81 0.13621037034162242 1105 82-83 0.19898728753721429 1105 84-85 0.32142443026618395 1105 86-87 0.3300001378401838 1105 88-89 0.3218602188302704 1105 90-91 0.43135944986243757 1105 92-93 0.5378178602664896 1105 94-95 0.5221335610177213 1105 96-97 0.4825836871682938 1105 98-99 0.7177163924377972 1105 100-101 1.3265299629999703 1106 1 0.1097241734646417 1106 2 -0.05013393250132481 1106 3 -0.009511509597054157 1106 4 0.10583285187715319 1106 5 0.08307792699464045 1106 6 0.036872571565083945 1106 7 -0.007651544220010464 1106 8 0.10313899592132003 1106 9 0.1061728466450802 1106 10-11 0.07523477536882694 1106 12-13 0.11618806909216062 1106 14-15 0.07173695282804715 1106 16-17 0.15157922844813498 1106 18-19 0.02734245004282343 1106 20-21 -0.07032260775442012 1106 22-23 0.06298157981047581 1106 24-25 0.008027574258100856 1106 26-27 0.14368545064845506 1106 28-29 0.17862428921012707 1106 30-31 0.1692181397537169 1106 32-33 0.032845313670968324 1106 34-35 0.07456925927633051 1106 36-37 0.05357028773412509 1106 38-39 0.21415619399850527 1106 40-41 0.4547398589135341 1106 42-43 0.22995667398622288 1106 44-45 0.26640049031099977 1106 46-47 0.42429986977845857 1106 48-49 0.3304714310320378 1106 50-51 0.3785329860474249 1106 52-53 0.37287100653367133 1106 54-55 0.4743276075015572 1106 56-57 0.24388723750644914 1106 58-59 0.2764862000877244 1106 60-61 0.30014896944042846 1106 62-63 0.26133433677295415 1106 64-65 0.27852501942747665 1106 66-67 0.4495357246280065 1106 68-69 0.35589942682954856 1106 70-71 0.3205766029488899 1106 72-73 0.39413278834942034 1106 74-75 0.5061131747555692 1106 76-77 0.42846195173802926 1106 78-79 0.3343381875890934 1106 80-81 0.33416279371017055 1106 82-83 0.3585632996099797 1106 84-85 0.4625552861886817 1106 86-87 0.5348732956114617 1106 88-89 0.5306060238456993 1106 90-91 0.47834429911384646 1106 92-93 0.5782190047875737 1106 94-95 0.6886918875925723 1106 96-97 0.7828318984069398 1106 98-99 1.0112444517550188 1106 100-101 1.4531695650852114 1107 1 0.10529385337738262 1107 2 0.029704594974589327 1107 3 0.059461437171776765 1107 4 0.08816094692928544 1107 5 0.022461392818627246 1107 6 -0.04425570123849809 1107 7 0.03950346012652517 1107 8 0.01276854575232278 1107 9 -0.04244860711634857 1107 10-11 0.05179268149272076 1107 12-13 0.13086827359609288 1107 14-15 0.12179912094679679 1107 16-17 0.12078468846977586 1107 18-19 0.13968121317405746 1107 20-21 0.08655351534892475 1107 22-23 0.10931889758905555 1107 24-25 0.0926188581531946 1107 26-27 0.08888773268972017 1107 28-29 0.1404728144566647 1107 30-31 0.16801891599487107 1107 32-33 0.2790048800053242 1107 34-35 0.13453792833994527 1107 36-37 0.12183701664371682 1107 38-39 0.1457937067351196 1107 40-41 0.07343084221716367 1107 42-43 0.20314807548454183 1107 44-45 0.3534276273307313 1107 46-47 0.062394637631776106 1107 48-49 0.28476626801091953 1107 50-51 0.11590522719688323 1107 52-53 0.2614576581183883 1107 54-55 0.3473236021091637 1107 56-57 0.2296005399876364 1107 58-59 0.26670613009435584 1107 60-61 0.2529655368326189 1107 62-63 0.24787280694347658 1107 64-65 0.5117998503915402 1107 66-67 0.6153734830446425 1107 68-69 0.44204828019807607 1107 70-71 0.4036260277254655 1107 72-73 0.4230099169154826 1107 74-75 0.37354171882461173 1107 76-77 0.3358194850028333 1107 78-79 0.4719204986271919 1107 80-81 0.4594483855192415 1107 82-83 0.608893591119358 1107 84-85 0.43747183427957737 1107 86-87 0.6461415102717645 1107 88-89 0.7519230303797109 1107 90-91 0.9006947477566953 1107 92-93 0.9632813000853879 1107 94-95 1.111071512608845 1107 96-97 1.1848090091679353 1107 98-99 1.2907092224062815 1107 100-101 1.684966874574716 1108 1 0.20261410356387444 1108 2 0.023053722081677108 1108 3 0.08017805916228582 1108 4 0.05456775410042525 1108 5 0.10434637151889348 1108 6 0.06999394422133776 1108 7 0.05002189484877562 1108 8 -0.009858084517617272 1108 9 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-1.5298872034963367 2316 96-97 -1.471275053162497 2316 98-99 -1.2479297737576154 2316 100-101 -1.240908889356632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 24.0 5 66.0 6 224.0 7 496.0 8 907.0 9 1653.0 10 2772.0 11 4338.0 12 5902.0 13 8101.0 14 9378.0 15 12065.0 16 15499.0 17 20649.0 18 18995.0 19 12076.0 20 10428.0 21 11125.0 22 11422.0 23 12128.0 24 13694.0 25 15418.0 26 17916.0 27 20937.0 28 25377.0 29 30549.0 30 36199.0 31 43640.0 32 52968.0 33 66618.0 34 87216.0 35 124706.0 36 174231.0 37 266614.0 38 221459.0 39 20105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03612849832733 20.95055133988414 15.652719982282523 20.360600179506008 2 15.367093998768807 23.60991088748391 38.473843684974426 22.549151428772856 3 19.526041481182148 27.48141036310025 32.01095136932091 20.981596786396693 4 12.494067523753525 18.918076227868138 37.9844189371183 30.60343731126004 5 11.920331921247946 39.57239039859485 34.086994831723864 14.420282848433342 6 27.806091299721487 38.059866382142424 17.895954832745097 16.238087485391 7 24.621320865382028 36.04808776212823 22.09047017190917 17.240121200580568 8 25.079566044359296 33.43220188080392 20.920981960184633 20.567250114652143 9 25.48468240084134 17.860005916123324 22.54319169765848 34.11211998537686 10-11 23.920580041732652 27.600023548205865 27.438274992168765 21.04112141789271 12-13 26.24691020647561 26.036248247511995 27.826747709319203 19.890093836693193 14-15 22.625428719072087 27.043188427074334 28.656930985586897 21.674451868266683 16-17 19.68713454755306 30.224375537389985 29.095956501483066 20.99253341357388 18-19 19.413969464372798 28.774926393686755 32.18567324247893 19.625430899461517 20-21 21.258651603567408 26.85767362622747 31.776050747837235 20.107624022367887 22-23 20.507652803508392 27.339648710190634 31.927442454071915 20.225256032229062 24-25 21.094036177152926 27.488305802182722 31.909788239808822 19.50786978085553 26-27 20.44984929677887 27.037655091312267 33.1192405168172 19.39325509509166 28-29 19.520408808482586 28.59300923540284 32.33975409567985 19.546827860434725 30-31 19.984104961040074 27.61463215740086 32.08504827018805 20.316214611371024 32-33 20.04791769175253 27.97225236408724 32.06611522195274 19.913714722207484 34-35 20.956613605860976 27.523755979652737 31.73378039070032 19.78585002378596 36-37 20.443434351878338 27.25043733187756 32.407845913795555 19.89828240244855 38-39 20.094467446674884 27.81556023353725 32.18202647065665 19.907945849131213 40-41 20.032832129113057 27.920974657041626 31.9595736693157 20.08661954452962 42-43 19.862587790629004 28.07668335407903 30.96630111845034 21.094427736841627 44-45 20.21500827849467 27.535516123077013 30.99653015508913 21.25294544333919 46-47 20.98400057853315 28.15890183360849 30.48302793784548 20.37406965001288 48-49 20.719315229984055 28.00451784242498 30.31547855260423 20.960688374986734 50-51 19.637393837708466 28.590475837460634 30.78369402438621 20.98843630044469 52-53 20.349719928977446 29.164852703791915 29.45589835315186 21.029529014078776 54-55 19.643891529986533 28.313774028800037 29.82452952647029 22.217804914743137 56-57 20.54523511619988 28.456006631375402 29.25858184940803 21.74017640301668 58-59 21.14282537137591 28.0357832017949 29.383994586804103 21.437396840025087 60-61 20.396979210540565 28.51121712115029 30.109774538281542 20.98202913002761 62-63 19.88461992713794 28.525697050085853 30.033233095002316 21.55644992777389 64-65 19.585872916427427 29.23782708110143 29.48323330053013 21.69306670194101 66-67 20.021100090236864 28.52262663052346 28.823028535273604 22.63324474396607 68-69 19.778530175009276 28.376766164073874 29.285009032279635 22.559694628637214 70-71 20.465884317858972 28.368351031452516 28.849127425367914 22.316637225320598 72-73 20.236848436043 27.786083446410753 29.542314454310304 22.434753663235945 74-75 19.62013034443812 29.17588795058918 28.573987948190737 22.62999375678196 76-77 19.92952451043133 28.15765245569738 28.891169146992773 23.02165388687851 78-79 20.009148544857347 28.16125165485313 28.977342613368812 22.85225718692071 80-81 20.27670099960789 28.147681917770285 28.609455919243516 22.96616116337831 82-83 19.964452048422547 27.937739118300325 29.040808554184643 23.057000279092485 84-85 19.807675116160247 28.054089647438296 28.6561315094618 23.482103726939656 86-87 19.840875168384862 28.126083383565348 28.512527324456848 23.520514123592942 88-89 19.830159738536732 27.847719964144346 28.0896872695658 24.23243302775312 90-91 20.012002436968963 27.30277150497426 28.42534593650018 24.259880121556595 92-93 20.23922610373611 27.801903488274903 28.393627420697076 23.56524298729191 94-95 20.02966792923741 27.244818196717556 28.509448203407743 24.216065670637292 96-97 19.539013960695502 27.207166807726008 28.958422441771685 24.295396789806805 98-99 19.923817193242826 27.48889636681709 27.932133198536814 24.65515324140327 100-101 19.87286645017429 26.69415703159296 28.702804613206425 24.730171905026324 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5879.0 1 3779.0 2 1875.0 3 2053.0 4 1727.5 5 1643.0 6 2554.0 7 4408.5 8 6144.5 9 6536.0 10 5243.5 11 3872.5 12 3667.0 13 3804.0 14 3851.0 15 3864.0 16 3879.5 17 3992.0 18 4223.0 19 4452.5 20 4799.0 21 5313.5 22 5939.5 23 6670.5 24 7570.0 25 8858.0 26 10685.5 27 12757.5 28 14452.5 29 16395.0 30 18836.0 31 21142.5 32 23933.5 33 26690.5 34 29592.0 35 31834.5 36 34322.0 37 37391.5 38 38412.5 39 37994.5 40 37834.5 41 38916.5 42 40058.5 43 41723.5 44 43529.0 45 44517.5 46 46296.5 47 55357.5 48 72487.5 49 73105.0 50 58305.5 51 47250.0 52 39530.5 53 35996.0 54 33902.5 55 31022.0 56 27712.5 57 24149.5 58 21648.0 59 19487.0 60 16619.0 61 13644.0 62 11076.5 63 8489.5 64 6110.0 65 4598.5 66 3432.5 67 2706.0 68 2064.5 69 1418.0 70 1048.5 71 839.5 72 685.5 73 596.5 74 486.0 75 381.0 76 275.5 77 159.5 78 106.0 79 80.5 80 51.5 81 34.5 82 23.5 83 12.5 84 10.5 85 6.5 86 4.0 87 3.5 88 2.0 89 2.5 90 2.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23584545690423947 2 0.0 3 0.0 4 0.0 5 0.02863578120809564 6 0.002689146966242484 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0027618266139787673 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.633982386814168E-4 26-27 0.0013082336592531005 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00650482847239736 36-37 0.016679979155477028 38-39 0.013736453422157555 40-41 0.008321819665804444 42-43 0.00824914001806816 44-45 0.0034159434436053173 46-47 5.450973580221251E-4 48-49 0.0016716318979345174 50-51 3.6339823868141676E-5 52-53 0.0 54-55 0.0 56-57 0.0012718938353849585 58-59 2.907185909451334E-4 60-61 0.002580127494638059 62-63 9.81175244439825E-4 64-65 0.0013809133069893837 66-67 0.00563267269956196 68-69 1.816991193407084E-4 70-71 1.0901947160442503E-4 72-73 0.0 74-75 5.814371818902668E-4 76-77 0.05908855360959837 78-79 0.13896348647177378 80-81 8.358159489672587E-4 82-83 9.448354205716836E-4 84-85 0.0 86-87 0.10505843080279757 88-89 0.3523509322255017 90-91 0.3885453967981708 92-93 4.3607788641770014E-4 94-95 3.270584148132751E-4 96-97 1.816991193407084E-4 98-99 0.0 100-101 0.0018169911934070838 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1375901.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.72638680879803 #Duplication Level Percentage of deduplicated Percentage of total 1 79.16096412260265 42.53032578608333 2 12.469365861543439 13.398679470754079 3 3.6333070002402836 5.856133718700693 4 1.5156449210555982 3.257205011736929 5 0.7670978362114566 2.0606697534244356 6 0.47812756739630075 1.5412839977929984 7 0.3212083774665302 1.2080155873795244 8 0.23211792414030893 0.997668590209398 9 0.17246979787348576 0.8339561166047498 >10 1.106203739058939 11.276254401996992 >50 0.09205758230214213 3.407407099358762 >100 0.04480285361603492 4.233499015502551 >500 0.0018949761408238609 0.6676568858882945 >1k 0.003925307720277997 4.592150024309785 >5k 4.060663158908273E-4 1.5795855809148944 >10k+ 4.060663158908273E-4 2.5595089593426006 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12581 0.9143826481701808 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11921 0.8664140806642339 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10694 0.7772361528918141 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7571 0.5502576130114013 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7150 0.519659481314426 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7000 0.5087575341539835 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4442 0.32284299524457066 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4332 0.3148482339935795 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3899 0.2833779465237688 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3495 0.2540153688383103 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3259 0.2368629719725474 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3188 0.23170271698327133 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3008 0.2186203803907403 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2826 0.20539268450273676 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2742 0.19928759409288893 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2346 0.17050645358932076 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2342 0.1702157349983756 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2292 0.16658175261156144 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2232 0.16222097374738445 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2046 0.14870255926843573 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1879 0.1365650580964764 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1827 0.13278571641418968 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1731 0.1258084702315065 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1698 0.12341004185620913 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1522 0.11061842385462325 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 7.267964773628335E-5 0.0 0.0 7.267964773628335E-5 0.0 16-17 7.267964773628335E-5 0.0 0.0 2.5437876707699175E-4 0.0 18-19 7.267964773628335E-5 0.0 0.0 4.724177102858418E-4 0.0 20-21 7.267964773628335E-5 0.0 0.0 0.0013809133069893837 0.0 22-23 1.453592954725667E-4 0.0 0.0 0.0034159434436053177 0.0 24-25 2.1803894320885004E-4 0.0 0.0 0.006868226711078777 0.0 26-27 2.1803894320885004E-4 0.0 0.0 0.013336715359607995 0.0 28-29 2.1803894320885004E-4 0.0 0.0 0.03484989108954786 0.0 30-31 2.1803894320885004E-4 0.0 0.0 0.08372695419219842 0.0 32-33 2.1803894320885004E-4 0.0 0.0 0.17642984487982782 0.0 34-35 2.1803894320885004E-4 0.0 0.0 0.3143394764594255 0.0 36-37 2.907185909451334E-4 0.0 0.0 0.500435714488179 0.0 38-39 2.907185909451334E-4 0.0 0.0 0.7562680745198964 0.0 40-41 2.907185909451334E-4 0.0 0.0 1.0292164915935085 0.0 42-43 3.633982386814168E-4 0.0 0.0 1.2993304024054058 0.0 44-45 4.360778864177001E-4 0.0 0.0 1.6203200666327011 0.0 46-47 5.814371818902668E-4 0.0 0.0 2.0016338384811116 0.0 48-49 6.541168296265501E-4 0.0 0.0 2.3737536348908823 0.0 50-51 6.541168296265501E-4 0.0 0.0 2.7746545718042217 0.0 52-53 6.541168296265501E-4 0.0 0.0 3.1574219366073577 0.0 54-55 6.541168296265501E-4 0.0 0.0 3.577328601403735 0.0 56-57 6.541168296265501E-4 0.0 0.0 4.105309902383965 0.0 58-59 6.541168296265501E-4 0.0 0.0 4.616175146322301 0.0 60-61 6.541168296265501E-4 0.0 0.0 5.194923181246326 0.0 62-63 6.541168296265501E-4 0.0 0.0 5.762006132708676 0.0 64-65 6.541168296265501E-4 0.0 0.0 6.339300574677974 0.0 66-67 7.267964773628335E-4 0.0 0.0 7.001739223970329 0.0 68-69 8.721557728354002E-4 0.0 0.0 7.742853591937211 0.0 70-71 9.084955967035419E-4 0.0 0.0 8.530519274279182 0.0 72-73 9.448354205716836E-4 0.0 0.0 9.294055313572706 0.0 74-75 9.448354205716836E-4 0.0 0.0 10.102143976928573 0.0 76-77 9.448354205716836E-4 0.0 0.0 10.91786400329675 0.0 78-79 9.448354205716836E-4 0.0 0.0 11.879415742847776 0.0 80-81 0.001017515068307967 0.0 0.0 12.871529274271914 0.0 82-83 0.001017515068307967 0.0 0.0 13.82824781724848 0.0 84-85 0.001017515068307967 0.0 0.0 14.822287359337626 0.0 86-87 0.001017515068307967 0.0 0.0 15.81545474565394 0.0 88-89 0.001017515068307967 0.0 0.0 16.87781315661519 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCC 125 7.4612763E-6 26.568201 8 GAGTACT 9720 0.0 22.916246 12-13 GTACATG 9600 0.0 21.501423 1 AGTACTT 9880 0.0 21.392666 12-13 TACATGG 9675 0.0 20.895803 2 GTACTTT 10620 0.0 20.70615 14-15 ATGGGAG 1925 0.0 20.215454 5 ACATGGG 9775 0.0 20.050892 3 TAACGTG 60 0.0047891675 19.774477 34-35 CATGGGG 2415 0.0 18.8649 4 CATGGGA 6665 0.0 18.22805 4 ACTTTTT 12255 0.0 18.137209 16-17 AGAGTAC 16970 0.0 18.032337 10-11 TACTTTT 11845 0.0 17.70359 14-15 GCTTATA 580 0.0 17.22027 1 TAATACC 490 1.8189894E-12 16.46468 4 ATAGTGC 235 5.1883904E-5 16.150883 6 TCTATAC 265 8.007417E-6 16.117477 3 GTATCAA 26815 0.0 16.087114 1 GTAAGCG 105 4.0422057E-4 15.814406 94-95 >>END_MODULE