##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938793_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3949932 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.481759939158447 33.0 31.0 34.0 30.0 34.0 2 31.496056640975084 33.0 31.0 34.0 28.0 34.0 3 31.520353008608755 33.0 31.0 34.0 28.0 34.0 4 35.0607719322763 37.0 35.0 37.0 32.0 37.0 5 35.11414576250933 37.0 35.0 37.0 32.0 37.0 6 35.226700864723746 37.0 35.0 37.0 32.0 37.0 7 35.144687047777026 37.0 35.0 37.0 32.0 37.0 8 35.12582241922139 37.0 35.0 37.0 32.0 37.0 9 36.63728843939592 39.0 37.0 39.0 32.0 39.0 10-11 36.518713866466555 39.0 37.0 39.0 32.0 39.0 12-13 36.39669594312004 39.0 37.0 39.0 32.0 39.0 14-15 37.44613438914898 40.0 37.0 41.0 32.0 41.0 16-17 37.42142117889624 40.0 37.0 41.0 32.0 41.0 18-19 37.41498385288658 40.0 37.0 41.0 32.0 41.0 20-21 37.30732136654504 40.0 37.0 41.0 31.0 41.0 22-23 37.216882467850084 40.0 37.0 41.0 31.0 41.0 24-25 37.197954800234534 40.0 37.0 41.0 31.0 41.0 26-27 37.08458905115329 40.0 36.5 41.0 31.0 41.0 28-29 36.987700041418435 40.0 36.0 41.0 30.0 41.0 30-31 36.79052272292283 39.0 36.0 41.0 30.0 41.0 32-33 36.538680286141634 39.0 35.0 41.0 30.0 41.0 34-35 36.152916303369274 39.0 35.0 41.0 29.5 41.0 36-37 35.86078216030048 39.0 35.0 41.0 28.0 41.0 38-39 35.6787792802509 39.0 35.0 40.0 27.0 41.0 40-41 35.54491026680965 38.5 35.0 40.0 27.0 41.0 42-43 35.26489886408171 38.0 35.0 40.0 26.0 41.0 44-45 35.14331335324253 38.5 35.0 40.5 25.0 41.0 46-47 34.87655141911304 39.0 34.5 41.0 24.0 41.0 48-49 34.57240162615458 38.5 34.0 40.0 23.0 41.0 50-51 33.399083199406974 37.5 32.5 39.5 21.0 40.5 52-53 33.54503596517611 37.5 33.0 39.5 21.5 40.5 54-55 34.097096228492035 38.0 34.0 40.0 21.5 41.0 56-57 33.973785244910545 38.0 33.5 40.0 20.5 41.0 58-59 33.73894208811696 38.0 33.0 40.0 19.5 41.0 60-61 33.39171332569776 37.5 33.0 40.0 18.0 41.0 62-63 33.073705066315064 37.0 33.0 40.0 15.0 41.0 64-65 32.68656738900822 36.5 32.0 39.5 13.0 41.0 66-67 32.23060169137089 36.0 31.5 39.0 8.0 41.0 68-69 31.802064693771946 35.5 31.0 39.0 7.5 40.0 70-71 31.330640628750064 35.0 31.0 38.5 6.0 40.0 72-73 30.909157550054026 35.0 30.5 37.0 2.0 39.5 74-75 30.479867121763107 35.0 30.0 37.0 2.0 39.0 76-77 30.057601244780923 35.0 30.0 36.0 2.0 39.0 78-79 29.623233007555573 34.0 29.0 36.0 2.0 38.5 80-81 29.130531361046216 34.0 29.0 35.5 2.0 37.0 82-83 28.65124538852821 34.0 28.5 35.0 2.0 37.0 84-85 28.237124588473925 34.0 27.5 35.0 2.0 36.0 86-87 27.919293927085327 34.0 27.0 35.0 2.0 36.0 88-89 27.517192954207818 34.0 26.5 35.0 2.0 36.0 90-91 27.06098003712469 33.5 25.5 35.0 2.0 35.0 92-93 26.591308787087982 33.0 25.0 35.0 2.0 35.0 94-95 26.0563807174402 33.0 24.0 35.0 2.0 35.0 96-97 25.452702097150027 33.0 19.5 35.0 2.0 35.0 98-99 24.770206803560164 32.0 10.0 35.0 2.0 35.0 100-101 22.54840475734772 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3567691457345532 1101 2 0.29320466950610324 1101 3 0.39880630104093484 1101 4 0.33894358261245117 1101 5 0.17593822946253113 1101 6 0.10365193490705593 1101 7 0.24411328945085842 1101 8 0.3401964603544343 1101 9 0.4247544952504967 1101 10-11 0.47477793869409624 1101 12-13 0.4242130396635062 1101 14-15 0.6360621254954353 1101 16-17 0.6319510313984438 1101 18-19 0.628025339657782 1101 20-21 0.6889038108102667 1101 22-23 0.5863272412138869 1101 24-25 0.69562503282922 1101 26-27 0.6326745966097533 1101 28-29 0.5820976582153534 1101 30-31 0.5279287684623171 1101 32-33 0.4923390724855423 1101 34-35 0.5575124951823156 1101 36-37 0.6633934380886259 1101 38-39 0.6297424397730467 1101 40-41 0.638577035593002 1101 42-43 0.6024046375048897 1101 44-45 0.7468313502951744 1101 46-47 0.7907686275192916 1101 48-49 0.7822402881396116 1101 50-51 0.6592353962306206 1101 52-53 0.648170250810864 1101 54-55 0.7298679386829221 1101 56-57 0.6732559744292459 1101 58-59 0.6618012714421297 1101 60-61 0.4550791600089994 1101 62-63 0.5781253732364391 1101 64-65 0.6665332563594575 1101 66-67 0.6073805345671062 1101 68-69 0.4841547503496493 1101 70-71 0.43693445834173517 1101 72-73 0.31709764280277497 1101 74-75 0.34905015026142294 1101 76-77 0.36981245762175874 1101 78-79 0.25979699596792827 1101 80-81 0.1510400727469765 1101 82-83 0.09394812076162751 1101 84-85 0.13757855057412272 1101 86-87 0.22238070297225931 1101 88-89 0.06764413254952473 1101 90-91 0.10002413189173254 1101 92-93 -0.09896659385019646 1101 94-95 -0.0983957275423144 1101 96-97 -0.21897958260860761 1101 98-99 -0.1457779793837446 1101 100-101 0.10417130426333543 1102 1 0.3074170859386278 1102 2 0.31619428988475917 1102 3 0.35912245717954505 1102 4 0.3342608849793578 1102 5 0.23176056204726336 1102 6 0.24619438423801654 1102 7 0.2921648704006117 1102 8 0.28893584590844057 1102 9 0.3611570830891111 1102 10-11 0.3412701690244475 1102 12-13 0.37893837428440946 1102 14-15 0.3794105187132786 1102 16-17 0.47674252266513406 1102 18-19 0.4395546381322504 1102 20-21 0.4862697511265992 1102 22-23 0.4993278031700612 1102 24-25 0.4496668231634402 1102 26-27 0.4188072290870295 1102 28-29 0.45536088565612687 1102 30-31 0.4828219122588564 1102 32-33 0.4628735665187307 1102 34-35 0.5133600515520627 1102 36-37 0.48805780283131384 1102 38-39 0.4697369486970615 1102 40-41 0.519782103612684 1102 42-43 0.49654774901092225 1102 44-45 0.6982220058633715 1102 46-47 0.6130406548827025 1102 48-49 0.6623150533318736 1102 50-51 0.624886247651709 1102 52-53 0.596504128518113 1102 54-55 0.6148452136079783 1102 56-57 0.5668022357300799 1102 58-59 0.4785306594508896 1102 60-61 0.477541075812951 1102 62-63 0.5026688599209237 1102 64-65 0.486568650807385 1102 66-67 0.4767727224971523 1102 68-69 0.3663531486054872 1102 70-71 0.3486210504460807 1102 72-73 0.3335486900632212 1102 74-75 0.3261120256261698 1102 76-77 0.20127037210839305 1102 78-79 0.011494732522059792 1102 80-81 0.038825843692016804 1102 82-83 0.10390691740449753 1102 84-85 0.06896062464549146 1102 86-87 0.06347893845691743 1102 88-89 -0.05588590573247387 1102 90-91 -0.0256301142586004 1102 92-93 -0.1386589921587813 1102 94-95 -0.14163818762550662 1102 96-97 -0.03420971076301882 1102 98-99 -0.07502627677825302 1102 100-101 0.11170188894796595 1103 1 0.14826322539587977 1103 2 0.30137911534735906 1103 3 0.14220434442809804 1103 4 0.3349641426270651 1103 5 0.1461753653509561 1103 6 0.27291277901196764 1103 7 0.25817288501973934 1103 8 0.2450550867499004 1103 9 0.40140824139582776 1103 10-11 0.322543910714856 1103 12-13 0.37438878292926603 1103 14-15 0.5007944074582795 1103 16-17 0.449167088839701 1103 18-19 0.5453121779175731 1103 20-21 0.5288914812483299 1103 22-23 0.5072461965051218 1103 24-25 0.54616690230241 1103 26-27 0.5592474216105572 1103 28-29 0.4915031127705731 1103 30-31 0.4442416653452739 1103 32-33 0.4287484837825417 1103 34-35 0.5579197870840247 1103 36-37 0.5458040689109183 1103 38-39 0.4526150531029458 1103 40-41 0.49379574429299566 1103 42-43 0.5299452928251327 1103 44-45 0.6659333069080091 1103 46-47 0.5757518120383125 1103 48-49 0.6022296911631813 1103 50-51 0.5888195622794612 1103 52-53 0.6004592783066016 1103 54-55 0.6243616745133309 1103 56-57 0.6632870266593116 1103 58-59 0.6719876654078973 1103 60-61 0.5628555812663407 1103 62-63 0.7016188297042234 1103 64-65 0.5142457062622157 1103 66-67 0.5334263312340966 1103 68-69 0.5663741923765322 1103 70-71 0.5389835428919056 1103 72-73 0.649949881464412 1103 74-75 0.6410842190705885 1103 76-77 0.7296335983882862 1103 78-79 0.6157324443986631 1103 80-81 0.36382440480168654 1103 82-83 0.44157400854381024 1103 84-85 0.5732602088061896 1103 86-87 0.532386317086516 1103 88-89 0.3655452865320541 1103 90-91 0.28832676219827746 1103 92-93 0.26837430376340166 1103 94-95 0.21660053866877504 1103 96-97 0.25839493253607415 1103 98-99 0.3537324644804869 1103 100-101 0.5829636518763976 1104 1 0.2314261191591953 1104 2 0.3124813139849998 1104 3 0.28936824835924924 1104 4 0.3043551778600744 1104 5 0.19553813006060494 1104 6 0.21144539210193614 1104 7 0.205121419488826 1104 8 0.2027055461032461 1104 9 0.33306788046559177 1104 10-11 0.3576921006033089 1104 12-13 0.34434006359526137 1104 14-15 0.38578074210315805 1104 16-17 0.48001839019208603 1104 18-19 0.4700069289826061 1104 20-21 0.4995702967057909 1104 22-23 0.5519123567752828 1104 24-25 0.42036658449856645 1104 26-27 0.454892825136902 1104 28-29 0.4028268565974713 1104 30-31 0.5172033209704452 1104 32-33 0.6130314615094932 1104 34-35 0.4698908993817099 1104 36-37 0.47197768021892017 1104 38-39 0.4674682676837989 1104 40-41 0.4385063816122923 1104 42-43 0.4346487358488247 1104 44-45 0.5565500868247781 1104 46-47 0.40350323304623004 1104 48-49 0.3776951152289101 1104 50-51 0.4320348795005131 1104 52-53 0.5583557166774327 1104 54-55 0.5445372174260328 1104 56-57 0.5505774457109567 1104 58-59 0.5972431869411636 1104 60-61 0.5845152872781014 1104 62-63 0.6046129876369335 1104 64-65 0.5374346759015225 1104 66-67 0.5313890303668458 1104 68-69 0.5854145631249246 1104 70-71 0.6158803049469803 1104 72-73 0.5054484969464426 1104 74-75 0.3821872669527515 1104 76-77 0.29919332088615036 1104 78-79 0.3587206969973238 1104 80-81 0.3377066572449401 1104 82-83 0.40525722143405574 1104 84-85 0.4721017644541483 1104 86-87 0.46256496295758964 1104 88-89 0.43094860023956727 1104 90-91 0.3736831220028414 1104 92-93 0.40115309485922523 1104 94-95 0.5094752458303624 1104 96-97 0.684931769797192 1104 98-99 0.6433950059451448 1104 100-101 0.7764398000497472 1105 1 0.03243334759611116 1105 2 0.06154820088752189 1105 3 0.07609488254891872 1105 4 0.061286276890250235 1105 5 0.027961562065058843 1105 6 0.11450837163597782 1105 7 0.19418072279814425 1105 8 0.14321933454307612 1105 9 0.16210193975916098 1105 10-11 0.11472007224534053 1105 12-13 0.1068195544556616 1105 14-15 0.19304164303142812 1105 16-17 0.11301887001017974 1105 18-19 0.1308116988549628 1105 20-21 0.27941098930600106 1105 22-23 0.23745600236696163 1105 24-25 0.24323264382849885 1105 26-27 0.16016215631982078 1105 28-29 0.17885487823409107 1105 30-31 0.045488013474574984 1105 32-33 0.0892065775845694 1105 34-35 0.09727890340984402 1105 36-37 0.08785712705812188 1105 38-39 0.286160234204317 1105 40-41 0.2389281540564525 1105 42-43 0.20110081539604607 1105 44-45 0.2602552369805764 1105 46-47 0.38459797796674167 1105 48-49 0.4906279335531494 1105 50-51 0.4046084056181414 1105 52-53 0.3382113751871856 1105 54-55 0.3590210688525346 1105 56-57 0.2405810297378821 1105 58-59 0.24029767388966405 1105 60-61 0.4302457502733503 1105 62-63 0.41376306681665653 1105 64-65 0.34520571631467334 1105 66-67 0.14733230145368026 1105 68-69 0.18585221047096567 1105 70-71 0.0824625976464084 1105 72-73 0.04397730583534809 1105 74-75 0.04423680443797551 1105 76-77 0.07231513170277282 1105 78-79 0.05313604286566331 1105 80-81 -0.046693768163471816 1105 82-83 -0.06526068344249225 1105 84-85 -0.011772029751284663 1105 86-87 0.05367044048048086 1105 88-89 -0.08069328664672426 1105 90-91 -0.01462418493205675 1105 92-93 0.04081642357617454 1105 94-95 0.2846592356184807 1105 96-97 0.1886593201212854 1105 98-99 0.2924411158799778 1105 100-101 0.4764774583036875 1106 1 0.18633940910874003 1106 2 0.22803086366823422 1106 3 0.2094713216877011 1106 4 0.2679596346073154 1106 5 0.1806954030175021 1106 6 0.12541735726630066 1106 7 0.17223118232533352 1106 8 0.24787025794019968 1106 9 0.24845091020595333 1106 10-11 0.2449652284717203 1106 12-13 0.23721670689902652 1106 14-15 0.3483660142174543 1106 16-17 0.3292634858055692 1106 18-19 0.4581613673584499 1106 20-21 0.4318852303703906 1106 22-23 0.33033357222893045 1106 24-25 0.3526408075533496 1106 26-27 0.2029104087654261 1106 28-29 0.3146058360515127 1106 30-31 0.2929122807563047 1106 32-33 0.3929361948114476 1106 34-35 0.32182412107807323 1106 36-37 0.5057582603547885 1106 38-39 0.4997872982164395 1106 40-41 0.4340510860232598 1106 42-43 0.48918592041015074 1106 44-45 0.46903611473057794 1106 46-47 0.5253923150219819 1106 48-49 0.4866535139535202 1106 50-51 0.5525084451854028 1106 52-53 0.46544636294707686 1106 54-55 0.39844309720586324 1106 56-57 0.49455416259704776 1106 58-59 0.4534861202470566 1106 60-61 0.498443477246326 1106 62-63 0.3066829382712868 1106 64-65 0.2884620703999161 1106 66-67 0.28804123111079605 1106 68-69 0.40219236674559156 1106 70-71 0.34914229256024143 1106 72-73 0.25168584625347634 1106 74-75 0.3279008300698436 1106 76-77 0.192706107791917 1106 78-79 0.3985525125001921 1106 80-81 0.2996197204150981 1106 82-83 0.30861607255613066 1106 84-85 0.28506660977802767 1106 86-87 0.39550607225396206 1106 88-89 0.39761693361107575 1106 90-91 0.5197877585929014 1106 92-93 0.6006374701465305 1106 94-95 0.6681358796706824 1106 96-97 0.7085238585192251 1106 98-99 0.7893677362883267 1106 100-101 0.9084896308684556 1107 1 0.07510493246258321 1107 2 0.07149540554399181 1107 3 0.10981631829369931 1107 4 0.1751562078443385 1107 5 0.10920178119452828 1107 6 0.08187169890066315 1107 7 -0.08984563247534538 1107 8 0.047754431138365305 1107 9 0.10450856705238465 1107 10-11 -0.06300305183668797 1107 12-13 0.03175451740147395 1107 14-15 0.12707315570693112 1107 16-17 0.03719711868302511 1107 18-19 0.08289608027995854 1107 20-21 0.08262155115907177 1107 22-23 0.10893177238606455 1107 24-25 -0.01128568330013735 1107 26-27 0.1790063224122136 1107 28-29 -0.041757422454466564 1107 30-31 0.10334656523811958 1107 32-33 0.24390250285728854 1107 34-35 0.1637991378280006 1107 36-37 0.1933254919338765 1107 38-39 0.28370262140434477 1107 40-41 0.2972043921018752 1107 42-43 0.3564678357402613 1107 44-45 0.3168708232189843 1107 46-47 0.3479910232575989 1107 48-49 0.457249037895302 1107 50-51 0.6056873784155314 1107 52-53 0.5167093435504881 1107 54-55 0.49075133609455435 1107 56-57 0.3614982183146509 1107 58-59 0.4116340358519537 1107 60-61 0.21630098952933707 1107 62-63 0.28950369189377767 1107 64-65 0.21563772941637183 1107 66-67 0.28232207840711254 1107 68-69 0.400802759528613 1107 70-71 0.5359403951889554 1107 72-73 0.3529685243301053 1107 74-75 0.43688143159577564 1107 76-77 0.2949792873791104 1107 78-79 0.32287107896431166 1107 80-81 0.3251983592363814 1107 82-83 0.3991572610258949 1107 84-85 0.3082201170758374 1107 86-87 0.36247773273052175 1107 88-89 0.32044314530436324 1107 90-91 0.5078680362502475 1107 92-93 0.4643766699417924 1107 94-95 0.5959814099791707 1107 96-97 0.5426045744571404 1107 98-99 0.694086232199961 1107 100-101 0.7472542896085521 1108 1 0.06389019420326747 1108 2 0.0862214638207277 1108 3 0.1006975668328316 1108 4 0.024002196870505088 1108 5 0.10022217643721376 1108 6 0.12888920178625796 1108 7 0.1707536480121874 1108 8 0.06320488727845941 1108 9 0.011013198743277997 1108 10-11 0.10057118891177907 1108 12-13 0.2363857284744313 1108 14-15 0.007191291445195702 1108 16-17 0.05882788813812567 1108 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-2.4435496976905036 2316 86-87 -2.7343668443333975 2316 88-89 -2.8703727923271245 2316 90-91 -2.9721761138949283 2316 92-93 -3.2513735652537044 2316 94-95 -3.2486474253795947 2316 96-97 -3.4229296367254136 2316 98-99 -3.780903600722141 2316 100-101 -3.9917347636170533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13263.0 3 4349.0 4 4344.0 5 4996.0 6 5666.0 7 7121.0 8 8671.0 9 10991.0 10 13417.0 11 17354.0 12 22434.0 13 28165.0 14 29766.0 15 30176.0 16 36947.0 17 46394.0 18 40076.0 19 29326.0 20 29685.0 21 31573.0 22 34409.0 23 38165.0 24 43232.0 25 49060.0 26 56722.0 27 65533.0 28 75422.0 29 88764.0 30 105034.0 31 126390.0 32 153150.0 33 191505.0 34 252020.0 35 357040.0 36 496949.0 37 697138.0 38 606523.0 39 98130.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.98920934459803 18.894044041048716 14.186623157813589 20.93012345653967 2 14.599801676650834 22.478428597892442 39.45273804567114 23.46903167978559 3 21.608390881869177 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29.11104735116865 20.85034057729371 48-49 22.091345520894006 27.308623670787036 29.1477098619566 21.45232094636236 50-51 20.326511736519542 27.839794606774387 30.468361878117854 21.36533177858822 52-53 21.461352861319103 28.73912893606829 28.266324387599752 21.53319381501285 54-55 20.48275840140413 27.302539578570496 29.150902540850428 23.063799479174946 56-57 21.934686223177994 27.1138437578043 28.62047147014109 22.330998548876615 58-59 23.3683674972899 26.70765010823244 28.269256024167994 21.654726370309668 60-61 21.77810617836651 27.281459511854894 30.261987161621256 20.678447148157343 62-63 21.06031028825706 27.482089241540624 29.862933532930246 21.594666937272066 64-65 20.59496535483576 28.934637187840817 28.870533424979193 21.59986403234422 66-67 21.366395228869006 27.55253091045097 27.97023386225751 23.110839998422513 68-69 20.634132817830743 27.635912640225108 28.740626478652754 22.989328063291396 70-71 22.102125606618245 27.40320316825717 28.30814323926001 22.186527985864572 72-73 21.678964688988287 26.392497712368122 29.878895764258594 22.049641834384992 74-75 20.450845827253488 29.039910030800026 28.5035904106632 22.00565373128329 76-77 21.11232427572059 27.152883541072757 28.51845045317739 23.216341730029256 78-79 21.30650309344467 27.330732141728703 28.816302699464213 22.54646206536241 80-81 21.985889551439815 27.216953391778254 28.440494711283844 22.35666234549809 82-83 21.4734360552309 26.752957367440978 29.406738503238444 22.366868074089684 84-85 20.890246250391993 27.57950471169647 29.020672662037928 22.50957637587361 86-87 20.933107747273578 27.79407891281187 28.655606370215924 22.61720696969863 88-89 20.65441381762169 27.604321886274786 27.828785736681404 23.91247855942212 90-91 21.182086485719147 26.526571371948325 28.3447471769404 23.946594965392126 92-93 21.982176661648886 27.327982447860922 28.46423362418141 22.225607266308785 94-95 21.357357261090073 26.73396602669687 28.720711726605636 23.18796498560742 96-97 20.130475622992286 26.758188469255302 29.8711145192453 23.240221388507113 98-99 20.9707890666341 27.465400740831196 28.015147352139046 23.548662840395654 100-101 20.668644840616242 25.9540449756883 28.95020267167951 24.427107512015944 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8159.0 1 5073.5 2 2832.5 3 3597.0 4 2880.0 5 2575.5 6 4430.5 7 8792.0 8 13385.5 9 14085.5 10 10928.5 11 8351.0 12 8105.5 13 8332.0 14 8615.5 15 8932.5 16 9251.0 17 9529.0 18 9459.5 19 9582.0 20 10273.5 21 11442.0 22 12867.5 23 14645.5 24 16528.5 25 19430.5 26 23870.5 27 28813.0 28 33380.0 29 38568.5 30 45245.0 31 52156.0 32 60413.5 33 68827.5 34 77414.5 35 84726.5 36 94247.5 37 103639.0 38 106371.0 39 106986.0 40 106664.0 41 108399.0 42 111205.5 43 115260.0 44 120734.5 45 126082.5 46 133889.0 47 172398.0 48 259282.5 49 257441.5 50 174886.0 51 134742.0 52 116941.0 53 110064.0 54 105564.0 55 97441.0 56 87309.0 57 77247.0 58 69072.0 59 61226.5 60 52328.0 61 43503.5 62 35493.5 63 27668.0 64 20675.5 65 15543.0 66 11401.5 67 9063.5 68 6808.5 69 4569.5 70 3449.0 71 2691.0 72 2043.0 73 1639.0 74 1303.0 75 998.5 76 707.5 77 451.5 78 286.5 79 184.0 80 133.5 81 103.5 82 86.5 83 67.5 84 39.5 85 30.5 86 29.5 87 25.5 88 21.5 89 15.5 90 10.0 91 9.5 92 11.5 93 10.0 94 7.0 95 4.0 96 2.0 97 1.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16506613278405805 2 0.0974953492870257 3 0.08995091561069912 4 0.10635626132297973 5 0.1287110765451152 6 0.04010195618557484 7 0.043722271674550346 8 0.04746917162118234 9 0.048431213499371635 10-11 0.04662105575488388 12-13 0.05048188171340671 14-15 0.04872235775198155 16-17 0.04648181285146175 18-19 0.051152779338986085 20-21 0.05382371139553795 22-23 0.06786192774964228 24-25 0.08425461501615725 26-27 0.08654579369973964 28-29 0.07569750567857876 30-31 0.06346944706896221 32-33 0.05314015532419292 34-35 0.30043555180190445 36-37 0.4769196026665775 38-39 0.4554382202022718 40-41 0.4300074026590837 42-43 0.46028640493051526 44-45 0.46952707033943875 46-47 0.4688435142680938 48-49 0.4854640535583904 50-51 0.47446386418804176 52-53 0.4816918367202271 54-55 0.472653706443554 56-57 0.45079257060627875 58-59 0.4294124557080983 60-61 0.39779165818550805 62-63 0.45589392424983516 64-65 0.49584397908622224 66-67 0.4968946300847711 68-69 0.507489749190619 70-71 0.5050213522663175 72-73 0.4951351061233459 74-75 0.4874387710978315 76-77 0.46898275717151583 78-79 0.4431342109180614 80-81 0.4491089973194475 82-83 0.46034969715934354 84-85 0.45876739143863743 86-87 0.46943846121907923 88-89 0.481261449564195 90-91 0.4855526626787499 92-93 0.4871729437367529 94-95 0.4920084700192307 96-97 0.49281861054823217 98-99 0.4863881200992827 100-101 0.47334992096066464 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3949932.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.178311156900676 #Duplication Level Percentage of deduplicated Percentage of total 1 71.60034664589637 29.4838135312667 2 14.17458867827146 11.673712462298875 3 5.543741014999338 6.8484567746674525 4 2.751847563134749 4.532657408444863 5 1.5560806162979706 3.20383859015698 6 0.9753852736367428 2.40988309774035 7 0.6714754054853759 1.9355156226897998 8 0.4708258568782432 1.5510250908197343 9 0.3392832496694931 1.2574000102693323 >10 1.6616298101393219 12.51491349061137 >50 0.14838801832616016 4.250731037286368 >100 0.09577156727371063 7.380092251815107 >500 0.006678606976467702 1.8647715156000702 >1k 0.0025972360408825163 2.4578590478486553 >5k 4.94711626834765E-4 1.2845943451940607 >10k+ 8.657453469608388E-4 7.350735723290271 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 60285 1.5262288059642546 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 34490 0.873179588914442 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27229 0.6893536395056927 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 22810 0.5774782958288902 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 22468 0.5688199189251866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19343 0.4897046328898827 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17195 0.4353239498806562 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14048 0.3556516922316637 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 13529 0.34251222552692046 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 13233 0.33501842563365647 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12585 0.3186130799213759 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11243 0.28463781148637496 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10191 0.2580044415954503 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10020 0.25367525314359846 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8693 0.22007973808156697 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7061 0.17876257110248986 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6380 0.16152176796967643 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6255 0.1583571565282643 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6151 0.15572419980900937 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5755 0.14569871076261565 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5132 0.1299262873386175 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5020 0.1270907954871122 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4743 0.12007801653294284 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4728 0.1196982631599734 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4695 0.11886280573944058 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4642 0.11752101048828183 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4598 0.11640706726090473 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4493 0.11374879365011854 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4233 0.10716640185198126 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.531689153129725E-5 2 0.0 0.0 0.0 0.0 2.531689153129725E-5 3 0.0 0.0 0.0 0.0 2.531689153129725E-5 4 0.0 0.0 0.0 0.0 2.531689153129725E-5 5 0.0 0.0 0.0 0.0 2.531689153129725E-5 6 0.0 0.0 0.0 0.0 2.531689153129725E-5 7 0.0 0.0 0.0 0.0 2.531689153129725E-5 8 0.0 0.0 0.0 0.0 2.531689153129725E-5 9 0.0 0.0 0.0 0.0 2.531689153129725E-5 10-11 0.0 0.0 0.0 3.7975337296945875E-5 2.531689153129725E-5 12-13 0.0 0.0 0.0 6.329222882824311E-5 2.531689153129725E-5 14-15 2.531689153129725E-5 0.0 0.0 1.6455979495343212E-4 2.531689153129725E-5 16-17 2.531689153129725E-5 0.0 0.0 2.784858068442697E-4 2.531689153129725E-5 18-19 3.7975337296945875E-5 0.0 0.0 3.9241181873510733E-4 2.531689153129725E-5 20-21 5.06337830625945E-5 0.0 0.0 8.35457420532809E-4 2.531689153129725E-5 22-23 5.06337830625945E-5 0.0 0.0 0.0018354746360190505 2.531689153129725E-5 24-25 7.595067459389174E-5 0.0 0.0 0.0033291712363655883 2.531689153129725E-5 26-27 7.595067459389174E-5 0.0 0.0 0.004759575607883882 2.531689153129725E-5 28-29 7.595067459389174E-5 0.0 0.0 0.009455858986939522 2.531689153129725E-5 30-31 7.595067459389174E-5 0.0 0.0 0.021658600705024796 2.531689153129725E-5 32-33 7.595067459389174E-5 0.0 0.0 0.046836249332899905 2.531689153129725E-5 34-35 7.595067459389174E-5 0.0 0.0 0.08584957918262896 2.531689153129725E-5 36-37 7.595067459389174E-5 0.0 0.0 0.14277460979075082 2.531689153129725E-5 38-39 7.595067459389174E-5 0.0 0.0 0.2262064258321409 2.531689153129725E-5 40-41 7.595067459389174E-5 0.0 0.0 0.3250688872618567 2.531689153129725E-5 42-43 1.139260118908376E-4 0.0 0.0 0.4231718419456335 2.531689153129725E-5 44-45 1.2658445765648623E-4 0.0 0.0 0.541427042288323 2.531689153129725E-5 46-47 1.7721824071908072E-4 0.0 0.0 0.690606825636492 2.531689153129725E-5 48-49 2.2785202378167524E-4 0.0 0.0 0.8442676987857005 2.531689153129725E-5 50-51 2.2785202378167524E-4 0.0 0.0 1.0153845686457388 2.531689153129725E-5 52-53 2.2785202378167524E-4 0.0 0.0 1.1872356283601846 2.531689153129725E-5 54-55 2.2785202378167524E-4 0.0 0.0 1.373833777391611 2.531689153129725E-5 56-57 2.784858068442697E-4 0.0 0.0 1.6156480668527964 2.531689153129725E-5 58-59 2.784858068442697E-4 0.0 0.0 1.8654118602548095 2.531689153129725E-5 60-61 2.784858068442697E-4 0.0 0.0 2.1417583897646844 2.531689153129725E-5 62-63 2.784858068442697E-4 2.531689153129725E-5 0.0 2.41664919801151 2.531689153129725E-5 64-65 2.784858068442697E-4 2.531689153129725E-5 0.0 2.6863120681571226 2.531689153129725E-5 66-67 3.0380269837556695E-4 2.531689153129725E-5 0.0 3.0041782997783253 2.531689153129725E-5 68-69 3.0380269837556695E-4 2.531689153129725E-5 0.0 3.3760581194815504 2.531689153129725E-5 70-71 3.4177803567251284E-4 2.531689153129725E-5 0.0 3.7745966259672317 2.531689153129725E-5 72-73 3.544364814381615E-4 2.531689153129725E-5 0.0 4.158881722520793 2.531689153129725E-5 74-75 3.544364814381615E-4 2.531689153129725E-5 0.0 4.561508906988778 2.531689153129725E-5 76-77 3.544364814381615E-4 2.531689153129725E-5 0.0 4.974756527454144 2.531689153129725E-5 78-79 3.544364814381615E-4 2.531689153129725E-5 0.0 5.49194264610125 2.531689153129725E-5 80-81 4.3038715603205323E-4 2.531689153129725E-5 0.0 6.0500155445714 2.531689153129725E-5 82-83 4.557040475633505E-4 2.531689153129725E-5 0.0 6.573505569209798 2.531689153129725E-5 84-85 4.683624933289991E-4 2.531689153129725E-5 0.0 7.115350340208389 2.531689153129725E-5 86-87 4.810209390946477E-4 2.531689153129725E-5 0.0 7.657891325724089 2.531689153129725E-5 88-89 4.810209390946477E-4 2.531689153129725E-5 0.0 8.250711657820945 2.531689153129725E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 27030 0.0 24.413078 1 TACATGG 27175 0.0 23.799135 2 ACATGGG 27210 0.0 22.6541 3 TACCTGG 2805 0.0 22.634453 2 GAGTACT 19335 0.0 22.063301 12-13 AGTACTT 20125 0.0 21.526583 12-13 CATGGGG 7935 0.0 21.25696 4 GTACTTT 21195 0.0 20.205801 14-15 TATAACG 165 6.45169E-5 20.100744 2 CATGGGA 18480 0.0 19.946913 4 GTATAGG 1120 0.0 19.893408 1 GTAAGCG 490 0.0 18.926449 94-95 ATGGGAT 7110 0.0 18.792196 5 ACTTTTT 24120 0.0 18.276827 16-17 TACTTTT 23850 0.0 18.194702 14-15 ATGGGAG 6040 0.0 17.728401 5 ATGGGGA 4425 0.0 17.238943 5 AGAGTAC 40820 0.0 17.21857 10-11 GTACCGG 325 1.9593062E-7 16.04496 1 GTACCTG 4285 0.0 15.7096615 1 >>END_MODULE