##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938793_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3949932 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.994159139954814 33.0 31.0 34.0 30.0 34.0 2 32.194623603646846 33.0 31.0 34.0 30.0 34.0 3 32.28778495427263 34.0 31.0 34.0 30.0 34.0 4 35.90341504613244 37.0 35.0 37.0 35.0 37.0 5 35.82844464157864 37.0 35.0 37.0 35.0 37.0 6 35.882513673653115 37.0 35.0 37.0 35.0 37.0 7 35.82030121024868 37.0 35.0 37.0 33.0 37.0 8 35.797388917075025 37.0 35.0 37.0 33.0 37.0 9 37.42325361550528 39.0 37.0 39.0 34.0 39.0 10-11 37.345241006680624 39.0 37.0 39.0 34.0 39.0 12-13 37.286211762632874 39.0 37.0 39.0 33.5 39.0 14-15 38.42646708854735 40.0 38.0 41.0 33.0 41.0 16-17 38.40060968138186 40.0 38.0 41.0 33.0 41.0 18-19 38.47217382476458 40.0 38.0 41.0 33.5 41.0 20-21 38.55478410767577 40.0 38.0 41.0 34.0 41.0 22-23 38.5070446782375 40.0 38.0 41.0 34.0 41.0 24-25 38.41650514489869 40.0 38.0 41.0 34.0 41.0 26-27 38.23263438459193 40.0 38.0 41.0 33.5 41.0 28-29 38.09666368940022 40.0 37.0 41.0 33.0 41.0 30-31 37.88246671081932 40.0 37.0 41.0 33.0 41.0 32-33 37.636418297833984 40.0 36.5 41.0 33.0 41.0 34-35 37.53543959744117 40.0 36.0 41.0 32.0 41.0 36-37 37.42762052612551 40.0 36.0 41.0 32.0 41.0 38-39 37.32078514263031 40.0 36.0 41.0 32.0 41.0 40-41 37.20743281150156 40.0 36.0 41.0 31.0 41.0 42-43 37.00768089172168 40.0 36.0 41.0 31.0 41.0 44-45 36.717013482763754 40.0 35.5 41.0 30.5 41.0 46-47 36.38266671426242 39.0 35.0 41.0 30.0 41.0 48-49 36.048228551782664 39.0 35.0 41.0 29.0 41.0 50-51 35.79700308764809 39.0 35.0 41.0 28.0 41.0 52-53 35.63366090859286 39.0 35.0 41.0 28.0 41.0 54-55 35.424192619012175 39.0 35.0 40.0 27.5 41.0 56-57 35.14967232347291 38.0 34.5 40.0 27.0 41.0 58-59 34.85641841429169 38.0 34.0 40.0 26.0 41.0 60-61 34.447636187154615 38.0 34.0 40.0 26.0 41.0 62-63 34.1376254072222 37.0 34.0 40.0 25.5 41.0 64-65 33.79129653371248 37.0 33.0 39.5 24.5 41.0 66-67 33.35210555523487 36.0 33.0 39.0 23.0 40.5 68-69 32.89825672948294 35.5 32.0 39.0 22.0 40.0 70-71 32.385449546979544 35.0 32.0 38.0 21.5 40.0 72-73 32.021869743580396 35.0 31.5 37.0 20.5 39.5 74-75 31.62762143753361 35.0 31.0 37.0 19.5 39.0 76-77 29.584140182666438 33.0 28.5 35.0 15.5 37.0 78-79 30.651768562091704 34.0 30.5 36.0 17.5 38.0 80-81 30.66763440484545 34.0 31.0 35.5 17.0 37.0 82-83 30.35858427436219 34.0 30.5 35.0 12.5 37.0 84-85 29.96777539461439 34.0 30.0 35.0 7.5 36.5 86-87 29.507029108349208 34.0 30.0 35.0 2.0 36.0 88-89 28.98458783594249 34.0 29.0 35.0 2.0 35.5 90-91 28.548822612642446 34.0 29.0 35.0 2.0 35.0 92-93 28.298879828817306 34.0 29.0 35.0 2.0 35.0 94-95 27.925180737288642 34.0 28.5 35.0 2.0 35.0 96-97 27.390580268217278 33.0 27.0 35.0 2.0 35.0 98-99 26.712168082893577 33.0 25.5 35.0 2.0 35.0 100-101 23.60125351525039 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06422000003993844 1101 2 0.13021252612288947 1101 3 0.1469545143853992 1101 4 0.07126850413899177 1101 5 0.10145396780603022 1101 6 0.12461876905371838 1101 7 0.11428323074879643 1101 8 0.08237187767235099 1101 9 0.223379591453174 1101 10-11 0.203158605765708 1101 12-13 0.18239191760725504 1101 14-15 0.2884819973477235 1101 16-17 0.2276998427208028 1101 18-19 0.1585993535825807 1101 20-21 0.2504230474035509 1101 22-23 0.21362183702257198 1101 24-25 0.17217572016402727 1101 26-27 0.1929177608946091 1101 28-29 0.21027644460273365 1101 30-31 0.30580318295036335 1101 32-33 0.3029469017794142 1101 34-35 0.2417377042136195 1101 36-37 0.23104230440394957 1101 38-39 0.30406694689140323 1101 40-41 0.25677927342911033 1101 42-43 0.2670276269532721 1101 44-45 0.36180678107152886 1101 46-47 0.37520377902069413 1101 48-49 0.32151064665279705 1101 50-51 0.3113649330017054 1101 52-53 0.4379786980645761 1101 54-55 0.43280857374884363 1101 56-57 0.30361002465503617 1101 58-59 0.3798091781855888 1101 60-61 0.3771832187566915 1101 62-63 0.3090406323259671 1101 64-65 0.28878535762157753 1101 66-67 0.3110420116730026 1101 68-69 0.29310610833262274 1101 70-71 0.3731191696112859 1101 72-73 0.4648163526996427 1101 74-75 0.4133458166769195 1101 76-77 0.34873738317051206 1101 78-79 0.41482968815027377 1101 80-81 0.5523183324022085 1101 82-83 0.5275751902078376 1101 84-85 0.5324786473295937 1101 86-87 0.7252105618334319 1101 88-89 0.7660437015052288 1101 90-91 0.8234404215610134 1101 92-93 0.8356366081461424 1101 94-95 0.7467631477023282 1101 96-97 0.6487567649119264 1101 98-99 0.9067937736553517 1101 100-101 1.4878156940411351 1102 1 0.09990982998564135 1102 2 0.1109322222472855 1102 3 0.09957146517467663 1102 4 0.09887137221183906 1102 5 0.14517659272970462 1102 6 0.12445315766095177 1102 7 0.14629633900211303 1102 8 0.11459766368403734 1102 9 0.16963440037116584 1102 10-11 0.20564865995288528 1102 12-13 0.21819207625336645 1102 14-15 0.218012625311772 1102 16-17 0.3280529418989673 1102 18-19 0.21194285757882625 1102 20-21 0.18543491204987816 1102 22-23 0.10646095322983484 1102 24-25 0.11281570101289162 1102 26-27 0.1754109325522748 1102 28-29 0.16100242300495182 1102 30-31 0.17914590250718732 1102 32-33 0.2378616920466854 1102 34-35 0.21831015023776956 1102 36-37 0.18021749339636983 1102 38-39 0.19882682389670947 1102 40-41 0.2169215342241131 1102 42-43 0.15859662753010895 1102 44-45 0.13260815357002542 1102 46-47 0.06975009915448283 1102 48-49 0.08675653121983373 1102 50-51 0.10436705707432736 1102 52-53 0.13544945571810274 1102 54-55 0.052106189391103896 1102 56-57 -0.05332901831533832 1102 58-59 -0.01630145617569667 1102 60-61 -0.04655942965958815 1102 62-63 -0.1761355588554423 1102 64-65 -0.09957045057757341 1102 66-67 -0.0981001226649596 1102 68-69 -0.06664291838767866 1102 70-71 -0.18705964200355396 1102 72-73 -0.12069555826919043 1102 74-75 -0.008784470596967964 1102 76-77 -0.20483012561892977 1102 78-79 -0.1325727691738514 1102 80-81 -0.13317220935465102 1102 82-83 -0.05792863968241946 1102 84-85 0.032713167668557475 1102 86-87 -0.009726442946917047 1102 88-89 0.044483045740918215 1102 90-91 0.23690001583000964 1102 92-93 0.3145800561496479 1102 94-95 0.24201847259211817 1102 96-97 0.3324348458484927 1102 98-99 0.3878909812538609 1102 100-101 0.7738453012195698 1103 1 0.0585309413845323 1103 2 0.09072300759362406 1103 3 0.024245556515431588 1103 4 0.05276932610978946 1103 5 0.021050718603831342 1103 6 0.017315182467420698 1103 7 0.08470463852152221 1103 8 0.02254825219018386 1103 9 0.06330400237410316 1103 10-11 0.07004943407588371 1103 12-13 0.0859040967431639 1103 14-15 0.18810888707470497 1103 16-17 0.03608770548928675 1103 18-19 0.06690810837741168 1103 20-21 0.1332526550065083 1103 22-23 0.04629645209310951 1103 24-25 0.09258040647203813 1103 26-27 0.0972558057860411 1103 28-29 0.0490954256812941 1103 30-31 0.041989977156823954 1103 32-33 0.08130502854557164 1103 34-35 0.07772337076210079 1103 36-37 0.047855770756875415 1103 38-39 0.18844433087533474 1103 40-41 0.026764982956450467 1103 42-43 0.16143124147583165 1103 44-45 0.1680237410689429 1103 46-47 0.08585038261587385 1103 48-49 0.2625835334357234 1103 50-51 0.3004779163796272 1103 52-53 0.19590803006556 1103 54-55 0.23355835348215948 1103 56-57 0.24901511388990372 1103 58-59 0.02149639412215265 1103 60-61 0.14167710232694475 1103 62-63 0.067255438007777 1103 64-65 0.18092143408097883 1103 66-67 0.1401234593086258 1103 68-69 0.1305507058892772 1103 70-71 0.049191760914510496 1103 72-73 0.07562573874655243 1103 74-75 0.32388517457266985 1103 76-77 0.15063154234273668 1103 78-79 0.08430307617421562 1103 80-81 0.12785778431423012 1103 82-83 0.16955002876847303 1103 84-85 0.022837883487714095 1103 86-87 0.20538676174962234 1103 88-89 0.24658058811623818 1103 90-91 0.28342743388520475 1103 92-93 0.36113805062430515 1103 94-95 0.3948448129184605 1103 96-97 0.3509587602057387 1103 98-99 0.4949471195600026 1103 100-101 0.9710677033030812 1104 1 -0.00679827672246347 1104 2 0.06642673859916215 1104 3 0.04819503972323247 1104 4 0.0316282564869681 1104 5 0.015515009380955291 1104 6 0.034076342354310896 1104 7 0.09734039951287343 1104 8 -0.005959455852412532 1104 9 -0.04043364485890777 1104 10-11 0.025839299740354704 1104 12-13 0.06490780559221321 1104 14-15 0.12330391667094176 1104 16-17 0.151143406479612 1104 18-19 0.10028440272057537 1104 20-21 0.07117383172602842 1104 22-23 0.06388739738758886 1104 24-25 0.09852029439801413 1104 26-27 0.07719243665579967 1104 28-29 0.12545409222824588 1104 30-31 0.06359101047871007 1104 32-33 0.11117920647162549 1104 34-35 0.1426967462242601 1104 36-37 -0.012122747425628688 1104 38-39 0.21491847892686877 1104 40-41 0.26885217661150307 1104 42-43 0.3251519353204273 1104 44-45 0.2919255970160748 1104 46-47 0.23055905761590623 1104 48-49 0.1847425282872237 1104 50-51 0.15914561185287823 1104 52-53 0.16744127477294768 1104 54-55 0.14049503396775265 1104 56-57 0.20871166845104483 1104 58-59 0.17567463781622195 1104 60-61 0.1792454422284706 1104 62-63 0.05047311263712828 1104 64-65 0.29903881253794395 1104 66-67 0.12751838006505523 1104 68-69 0.19920297057634428 1104 70-71 0.18575500613643214 1104 72-73 0.09550413139312042 1104 74-75 0.15494622094693966 1104 76-77 0.09572394592263933 1104 78-79 0.18770328981106132 1104 80-81 0.149924458278889 1104 82-83 0.13726334665527062 1104 84-85 0.08106813271145086 1104 86-87 0.26676026579292156 1104 88-89 0.4233240595653456 1104 90-91 0.4271140621297924 1104 92-93 0.47160248237339886 1104 94-95 0.47830750181301696 1104 96-97 0.6233274278867746 1104 98-99 0.6727433851417359 1104 100-101 1.161136471108339 1105 1 -0.1373326278675009 1105 2 -0.10227639554148737 1105 3 -0.05329661670104002 1105 4 -0.08805623227301851 1105 5 -0.058635456120512686 1105 6 -0.08150404600617378 1105 7 -0.12485933597035626 1105 8 -0.08682802400119272 1105 9 -0.10134353320218992 1105 10-11 -0.01391100006479462 1105 12-13 -0.04004146289620536 1105 14-15 0.05111661490431629 1105 16-17 -0.07933182769954072 1105 18-19 -0.10174352671824494 1105 20-21 -0.04754066481883257 1105 22-23 -0.12477032609304928 1105 24-25 -0.10626886158312487 1105 26-27 -0.11951256351625972 1105 28-29 -0.09304539516499943 1105 30-31 -0.12474246244988052 1105 32-33 -0.1740530118527488 1105 34-35 -0.09070404732604942 1105 36-37 -0.10903369928993101 1105 38-39 -0.09379245956226612 1105 40-41 -0.048729001273748906 1105 42-43 -0.18580590236860672 1105 44-45 -0.28388877895744 1105 46-47 -0.21886308297015233 1105 48-49 -0.22507118022620176 1105 50-51 -0.2596839046865611 1105 52-53 -0.27881552724917213 1105 54-55 -0.2736733321899436 1105 56-57 -0.1722059852839024 1105 58-59 -0.12582831608423817 1105 60-61 -0.29287371757693137 1105 62-63 -0.3898761157257695 1105 64-65 -0.3566893000628397 1105 66-67 -0.39941746520366905 1105 68-69 -0.4443403270011217 1105 70-71 -0.48076771510093863 1105 72-73 -0.5903354885961392 1105 74-75 -0.628429640242139 1105 76-77 -0.5625399306569783 1105 78-79 -0.5786455541779354 1105 80-81 -0.5633009581856889 1105 82-83 -0.564656023127359 1105 84-85 -0.40305407611395694 1105 86-87 -0.3740808897441106 1105 88-89 -0.3343303376526201 1105 90-91 -0.2692727907244752 1105 92-93 -0.2551286772995489 1105 94-95 -0.3552718113393887 1105 96-97 -0.24449524978389192 1105 98-99 -0.20445032177446265 1105 100-101 0.2828335987574846 1106 1 -0.035622549794524616 1106 2 -0.09661473751206273 1106 3 -0.09962795880350939 1106 4 -0.033328908661012235 1106 5 -0.07899844560462554 1106 6 -0.08816968328047636 1106 7 -0.058292713374548555 1106 8 -0.02437768414086605 1106 9 -0.05922530105490864 1106 10-11 -0.084225575939044 1106 12-13 -0.012571162474479536 1106 14-15 -0.044908000440713636 1106 16-17 -0.13647034209333953 1106 18-19 -0.19748393326389646 1106 20-21 -0.10684540677910803 1106 22-23 -0.12006375117097434 1106 24-25 -0.11995045122228731 1106 26-27 -0.035100941676411423 1106 28-29 -0.07488627040586238 1106 30-31 -0.05002626914286168 1106 32-33 -0.040577011170782384 1106 34-35 -0.025296456200699424 1106 36-37 -4.6802888206798343E-4 1106 38-39 -0.059898750758065944 1106 40-41 -0.006826137311172431 1106 42-43 0.030891163541461708 1106 44-45 -0.0490287280668511 1106 46-47 0.07008912427227187 1106 48-49 -0.07139914463495955 1106 50-51 0.03398758403998414 1106 52-53 -0.052129173453444366 1106 54-55 0.016634777813500534 1106 56-57 -0.1703649813689978 1106 58-59 -0.20333094586005274 1106 60-61 -0.18214793161977383 1106 62-63 -0.14735722476736868 1106 64-65 -0.08299574373738494 1106 66-67 -0.07024931074334972 1106 68-69 -0.08874107588406588 1106 70-71 -0.11290341474997945 1106 72-73 -0.020185776343474515 1106 74-75 0.1043769337148781 1106 76-77 0.01162618110286573 1106 78-79 -0.018342784147407087 1106 80-81 -0.16082446532549 1106 82-83 0.05084678679212473 1106 84-85 -0.04993083532943032 1106 86-87 0.02062288651746158 1106 88-89 0.27385976538312207 1106 90-91 0.2768023291836492 1106 92-93 0.24476822262099773 1106 94-95 0.1819093585007039 1106 96-97 0.3058109944458778 1106 98-99 0.3177642872333628 1106 100-101 0.7383278196843897 1107 1 -0.07767616779433695 1107 2 -0.04370729299904497 1107 3 -0.12062197536196351 1107 4 -0.06239458189172353 1107 5 -0.025088992358064388 1107 6 -0.01115334899753151 1107 7 -0.038446400826423144 1107 8 0.0020671718946587703 1107 9 -0.08210889166061008 1107 10-11 -0.0364881443575058 1107 12-13 -0.049275996860068005 1107 14-15 -0.0966549761004174 1107 16-17 -0.0727109574971152 1107 18-19 0.03276578919509632 1107 20-21 -0.02478585910100861 1107 22-23 -0.14279649025790064 1107 24-25 -0.0627530448120126 1107 26-27 -0.01468128017112491 1107 28-29 0.005070096468301699 1107 30-31 -0.04801297672829463 1107 32-33 -0.03939814962499355 1107 34-35 -0.1232828179406269 1107 36-37 -0.0070408854810111166 1107 38-39 -0.03397634151028228 1107 40-41 -0.059268168103365326 1107 42-43 -0.10128108260872892 1107 44-45 -0.058653199591347516 1107 46-47 -0.10046125111158233 1107 48-49 -0.3326530357898392 1107 50-51 -0.20146120237244247 1107 52-53 -0.15154318541529932 1107 54-55 -0.1530780540920702 1107 56-57 -0.1479872684075545 1107 58-59 -0.10172072065489601 1107 60-61 -0.22684274025873918 1107 62-63 -0.21248480560011274 1107 64-65 -0.18169124595377895 1107 66-67 -0.1913869789460918 1107 68-69 -0.21551218998762067 1107 70-71 -0.2670676303676771 1107 72-73 -0.2181372076879562 1107 74-75 -0.19223217692455918 1107 76-77 -0.2295605279917119 1107 78-79 -0.19116215753195576 1107 80-81 -0.1791895215992696 1107 82-83 -0.06170114818011996 1107 84-85 -0.2407927483392278 1107 86-87 -0.25363460677549554 1107 88-89 0.003497655713001535 1107 90-91 0.0239096617056056 1107 92-93 0.10043853250799728 1107 94-95 0.28033315191649777 1107 96-97 0.20687971992032317 1107 98-99 0.22393215875239036 1107 100-101 0.6071474269862556 1108 1 -0.12155648268588237 1108 2 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-0.9450720616230299 2316 50-51 -0.9116037655525986 2316 52-53 -0.951768339106664 2316 54-55 -0.9926265035190838 2316 56-57 -0.9606376807745534 2316 58-59 -0.9130834908341185 2316 60-61 -0.8779878181402765 2316 62-63 -0.8991448674431979 2316 64-65 -0.8329351371910718 2316 66-67 -0.9043362355571674 2316 68-69 -0.9749141681803266 2316 70-71 -1.0152715779809398 2316 72-73 -1.178605277827085 2316 74-75 -1.215558913504534 2316 76-77 -1.1697598956200146 2316 78-79 -1.1334315101276822 2316 80-81 -1.0361745307404888 2316 82-83 -1.2788762663352742 2316 84-85 -1.3213119232820496 2316 86-87 -1.5780924550530209 2316 88-89 -1.8749702778328476 2316 90-91 -2.38533711195387 2316 92-93 -2.5334631080583385 2316 94-95 -2.469588374513936 2316 96-97 -2.532567675255457 2316 98-99 -3.0660013727969684 2316 100-101 -3.796160410624495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 18.0 4 55.0 5 118.0 6 321.0 7 802.0 8 1575.0 9 2796.0 10 4689.0 11 7286.0 12 10201.0 13 14469.0 14 17076.0 15 22715.0 16 29862.0 17 40700.0 18 38540.0 19 24909.0 20 22190.0 21 22934.0 22 24494.0 23 26716.0 24 31338.0 25 37360.0 26 45304.0 27 55217.0 28 67402.0 29 82643.0 30 100362.0 31 122209.0 32 149561.0 33 189100.0 34 251840.0 35 357300.0 36 526926.0 37 822412.0 38 720298.0 39 78190.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.67175146455447 19.792536382890415 14.7702881681994 20.765423984355717 2 15.02286621643107 23.134018509685735 38.51914412703813 23.32397114684506 3 21.652372749708096 26.265869893456394 30.023504202097655 22.05825315473785 4 12.483227559360516 17.379615649079525 38.57856287146209 31.558593920097866 5 12.380111860153542 39.716810314709214 33.646452647792415 14.256625177344825 6 29.104880792884536 37.62325528642012 17.387742371261076 15.88412154943427 7 26.965805993622165 34.42920030015706 21.170541670084447 17.43445203613632 8 25.63342862611306 32.80360269493247 19.77854302301913 21.784425655935344 9 25.70345514808863 18.69832189516174 21.930276268047148 33.667946688702486 10-11 24.99997468310847 26.73257413039009 26.775916648691673 21.491534537809763 12-13 27.80157987529912 24.683855317002926 26.901728941156456 20.612835866541502 14-15 23.15191380897455 25.9796517603883 28.23182167555629 22.636612755080858 16-17 20.67853690195695 29.42324540555512 28.231048686260735 21.667169006227198 18-19 20.363817034500656 29.171984278182496 30.144800217725653 20.319398469591196 20-21 22.453034945036386 26.315114578569275 29.483258127387778 21.74859234900656 22-23 21.255289964485467 26.76051132019488 29.9645285032755 22.019670212044158 24-25 22.57055311331942 26.779980009782445 29.871071198187714 20.778395678710417 26-27 21.847610232500674 25.818066372922615 31.816333681356905 20.517989713219798 28-29 20.303590036486703 28.603353171649538 30.452714122673502 20.640342669190254 30-31 21.080945190955187 26.720282779551646 30.16374712273528 22.03502490675789 32-33 21.268277529841022 27.11849216644742 30.39900433728986 21.2142259664217 34-35 22.599428428558316 26.408841317715865 30.147386943738507 20.84434330998731 36-37 21.962021830768812 25.93041576960645 31.29288718026305 20.81467521936169 38-39 21.17951507021559 27.332984932949383 31.034270596197626 20.4532294006374 40-41 21.206506923407726 27.529386773397917 30.325372755299675 20.938733547894685 42-43 20.579469406448876 27.84160374580707 29.03549873246843 22.543428115275624 44-45 21.55627876136609 26.53940281046009 29.21655409070442 22.687764337469396 46-47 22.536929100885956 27.656305302508997 29.054289436654308 20.752476159950742 48-49 22.13940219960832 27.44276133466146 29.096701828044825 21.321134637685397 50-51 20.36177758851904 27.92608343525541 30.35363057363542 21.358508402590132 52-53 21.513117187840194 28.874598347515857 28.112192817496606 21.500091647147343 54-55 20.578139066697858 27.389623416301852 28.95994158886786 23.072295928132434 56-57 21.96455533918052 27.24707665853488 28.53014937978679 22.258218622497804 58-59 23.37568401762417 26.871213606667382 28.12508821389009 21.628014161818356 60-61 21.757676297073157 27.522755308517112 30.01285474768048 20.706713646729256 62-63 21.05012187551583 27.64989371968935 29.65691561272447 21.643068792070345 64-65 20.603038229518887 29.084437402973013 28.63202455130873 21.680499816199365 66-67 21.383871274406214 27.738363886290013 27.755908676455714 23.121856162848058 68-69 20.702571238729636 27.86880597523156 28.45671877466436 22.97190401137445 70-71 22.09942485412485 27.74337367012944 28.01061048250353 22.146590993242178 72-73 21.730450043190615 26.707219263521498 29.53392615366543 22.028404539622453 74-75 20.498960488433728 29.362948526708816 28.10176225818571 22.03632872667175 76-77 21.146443063998554 27.47587264277953 28.184225114752643 23.19345917846927 78-79 21.33384103680918 27.697930276796324 28.520698624971157 22.44753006142334 80-81 21.944234208677848 27.673221085788725 28.10206407429194 22.28048063124149 82-83 21.431128434616088 27.282444356004103 29.03508212293275 22.251345086447056 84-85 20.812029169109746 28.073242779875706 28.629644763504793 22.48508328750976 86-87 20.889094436015913 28.313664577768634 28.166324813565502 22.63091617264995 88-89 20.59131066048089 28.196441777584617 27.35077243244431 23.86147512949018 90-91 21.138641537064014 27.181635478925998 27.82033972490336 23.85938325910663 92-93 21.84566721654955 28.114281461047934 27.899923340452442 22.14012798195007 94-95 21.21512740157646 27.52700901268794 28.171222716140775 23.086640869594827 96-97 19.89897289319051 27.61880590288942 29.298569848073257 23.183651355846813 98-99 20.773326369485385 28.304155764029353 27.374416238694245 23.54810162779102 100-101 20.33989704048354 27.100811615072985 28.34485513817811 24.214436206265372 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6834.0 1 4414.5 2 2683.0 3 3361.0 4 2732.0 5 2492.5 6 4390.0 7 8867.0 8 12841.5 9 13279.5 10 10497.5 11 7648.5 12 6967.0 13 7159.5 14 7552.5 15 7691.5 16 7671.0 17 7839.5 18 8307.5 19 8874.0 20 9664.5 21 10874.0 22 12538.5 23 14685.5 24 16763.0 25 19517.0 26 23910.0 27 29008.5 28 33388.0 29 38633.0 30 45638.0 31 52614.5 32 61054.5 33 69603.5 34 77714.5 35 84772.5 36 94641.0 37 104285.0 38 107187.5 39 107820.5 40 107692.0 41 109762.0 42 112510.5 43 116379.0 44 121939.5 45 126823.5 46 132949.0 47 176484.5 48 270978.5 49 264707.0 50 174744.0 51 133122.5 52 114873.5 53 108230.0 54 104441.5 55 96643.0 56 87040.0 57 77219.5 58 68867.5 59 60675.0 60 51552.5 61 42273.5 62 34237.0 63 26750.0 64 19771.5 65 14888.5 66 10798.0 67 8414.5 68 6277.5 69 4051.0 70 2934.0 71 2243.0 72 1737.5 73 1479.5 74 1181.0 75 864.0 76 570.0 77 313.0 78 193.5 79 128.0 80 69.5 81 39.5 82 28.5 83 18.0 84 7.5 85 4.0 86 5.5 87 6.0 88 3.5 89 4.0 90 3.5 91 1.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3913738261823242 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.02535132250378E-4 16-17 0.0020380097682694285 18-19 1.7721824071908072E-4 20-21 1.2658445765648624E-5 22-23 0.0 24-25 0.0 26-27 2.02535132250378E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007455824555967039 36-37 0.017455996710829452 38-39 0.011468551863677652 40-41 0.008190014410374659 42-43 0.003265879007537345 44-45 0.0020380097682694285 46-47 0.0016709148410656183 48-49 3.797533729694587E-5 50-51 4.8102093909464767E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.9241181873510733E-4 60-61 7.468483001732688E-4 62-63 0.0 64-65 0.0 66-67 0.0010506509985488358 68-69 6.70897625579377E-4 70-71 0.0016329395037686725 72-73 0.0 74-75 0.0 76-77 0.09986247864520198 78-79 0.036279105564348955 80-81 3.797533729694587E-5 82-83 0.0 84-85 0.0 86-87 0.151559571152111 88-89 0.33039809292919475 90-91 0.28096686221433687 92-93 0.0 94-95 1.2658445765648624E-5 96-97 5.06337830625945E-5 98-99 2.1519357801602661E-4 100-101 7.595067459389175E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3949932.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.09027031070307 #Duplication Level Percentage of deduplicated Percentage of total 1 69.23041041523109 27.062354568522963 2 15.347402750558661 11.998682441731317 3 6.023684903103105 7.064024133864047 4 2.958789294639458 4.6263949327948355 5 1.7108153341597487 3.343811693200019 6 1.0613499668694946 2.4893074259510573 7 0.6935977543257625 1.8979046592443471 8 0.5159722794412955 1.6135596700951889 9 0.36004245954040975 1.2666741360088491 >10 1.8195692551811118 12.974586311441353 >50 0.16049133095602802 4.348956580541455 >100 0.10607846945897327 7.8003323724103995 >500 0.007518193619894401 2.0175933057864732 >1k 0.0027221045822496083 2.3250358511523856 >5k 6.48120138630859E-4 1.6311556287361373 >10k+ 9.073681940832028E-4 7.539626288519186 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 64707 1.638180100315651 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 32732 0.8286724936024216 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26029 0.658973369668136 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 21997 0.5568956630139456 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 21533 0.5451486253434237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20468 0.5181861358625921 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17925 0.4538052806985032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15243 0.38590537761156396 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 14694 0.37200640416088177 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 14383 0.3641328508946483 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 13519 0.3422590566116075 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12635 0.3198789244979407 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11270 0.28532136755772 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10460 0.2648146854173692 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9890 0.2503840572445298 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7681 0.19445904385189416 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7353 0.18615510342962865 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6165 0.15607863629044755 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6112 0.15473684103928878 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5946 0.15053423704509342 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5601 0.14179990946679588 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5369 0.13592639063153492 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5178 0.13109086434905715 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5088 0.1288123441112404 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4867 0.1232173110828237 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4832 0.1223312198792283 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4766 0.12066030503816269 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4664 0.11807798210197037 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4478 0.11336904027714907 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.531689153129725E-5 0.0 0.0 0.0 0.0 3 2.531689153129725E-5 0.0 0.0 0.0 0.0 4 2.531689153129725E-5 0.0 0.0 0.0 0.0 5 2.531689153129725E-5 0.0 0.0 0.0 0.0 6 2.531689153129725E-5 0.0 0.0 0.0 0.0 7 2.531689153129725E-5 0.0 0.0 0.0 0.0 8 2.531689153129725E-5 0.0 0.0 0.0 0.0 9 2.531689153129725E-5 0.0 0.0 0.0 0.0 10-11 2.531689153129725E-5 0.0 0.0 3.7975337296945875E-5 0.0 12-13 3.7975337296945875E-5 0.0 0.0 6.329222882824311E-5 0.0 14-15 8.860912035954036E-5 0.0 0.0 1.6455979495343212E-4 0.0 16-17 1.3924290342213485E-4 0.0 0.0 2.784858068442697E-4 0.0 18-19 1.6455979495343212E-4 0.0 0.0 4.05070264500756E-4 0.0 20-21 1.7721824071908075E-4 0.0 0.0 8.860912035954036E-4 0.0 22-23 2.02535132250378E-4 0.0 0.0 0.0019240837563785909 0.0 24-25 2.02535132250378E-4 0.0 0.0 0.00340512191095948 0.0 26-27 2.02535132250378E-4 0.0 0.0 0.004822867836712126 0.0 28-29 2.784858068442697E-4 0.0 0.0 0.009595101890361658 0.0 30-31 3.291195899068642E-4 0.0 0.0 0.022038354077994253 0.0 32-33 3.291195899068642E-4 0.0 0.0 0.04748183006694799 0.0 34-35 3.4177803567251284E-4 0.0 0.0 0.08734327578297552 0.0 36-37 3.544364814381615E-4 0.0 0.0 0.14545820029306833 0.0 38-39 3.7975337296945874E-4 0.0 0.0 0.23073814941624313 0.0 40-41 3.7975337296945874E-4 0.0 0.0 0.33130950102432144 0.0 42-43 3.7975337296945874E-4 0.0 0.0 0.4307289340677257 0.0 44-45 3.7975337296945874E-4 0.0 0.0 0.5504018803361679 0.0 46-47 4.557040475633505E-4 0.0 0.0 0.700872825152433 0.0 48-49 4.557040475633505E-4 0.0 0.0 0.8561539793596447 0.0 50-51 4.557040475633505E-4 0.0 0.0 1.0286759366996696 0.0 52-53 4.557040475633505E-4 0.0 0.0 1.201767524099149 0.0 54-55 4.557040475633505E-4 0.0 0.0 1.3904289997903763 0.0 56-57 4.557040475633505E-4 0.0 0.0 1.6347749784046917 0.0 58-59 4.557040475633505E-4 0.0 0.0 1.8872856545378505 0.0 60-61 4.557040475633505E-4 0.0 0.0 2.1662271654296834 0.0 62-63 4.557040475633505E-4 0.0 0.0 2.443371177022794 0.0 64-65 4.557040475633505E-4 0.0 0.0 2.715654345441896 0.0 66-67 5.063378306259449E-4 0.0 0.0 3.036001632433166 0.0 68-69 5.063378306259449E-4 0.0 0.0 3.4108308699997876 0.0 70-71 5.189962763915935E-4 0.0 0.0 3.8129770335286786 0.0 72-73 5.443131679228908E-4 0.0 0.0 4.2019837303528265 0.0 74-75 5.569716136885394E-4 0.0 0.0 4.61061101811373 0.0 76-77 5.569716136885394E-4 0.0 0.0 5.028630872632744 0.0 78-79 5.569716136885394E-4 0.0 0.0 5.549867693924858 0.0 80-81 6.076053967511339E-4 0.0 0.0 6.111788759907766 0.0 82-83 6.329222882824312E-4 0.0 0.0 6.6392029027335155 0.0 84-85 6.708976255793771E-4 0.0 0.0 7.183997091595501 0.0 86-87 7.08872962876323E-4 0.0 0.0 7.729626737878019 0.0 88-89 7.08872962876323E-4 0.0 0.0 8.327067402679337 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATACG 35 0.0081829345 27.285263 82-83 TACCTGG 2905 0.0 22.70544 2 GTACATG 28340 0.0 22.675318 1 TACATGG 28660 0.0 21.98787 2 GAGTACT 20075 0.0 21.381138 12-13 ACATGGG 28310 0.0 21.287523 3 CATGGGG 7860 0.0 21.190727 4 TATAACG 180 5.2188116E-6 21.090107 2 AGTACTT 20560 0.0 21.084496 12-13 GTACTTT 21850 0.0 19.731098 14-15 CATGGGA 19245 0.0 18.394255 4 ATGGGAG 6305 0.0 18.13816 5 TACTTTT 24305 0.0 17.767385 14-15 ACTTTTT 24795 0.0 17.396908 16-17 GTAAGCG 375 0.0 17.083094 94-95 ATGGGAT 7125 0.0 16.383682 5 AGAGTAC 43590 0.0 16.37863 10-11 GTACCTG 4255 0.0 16.0095 1 AGCGTAC 240 6.004097E-5 15.878886 1 CGTCGAG 90 0.0026345528 15.820585 38-39 >>END_MODULE