##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938792_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3957465 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06259739504961 33.0 31.0 34.0 30.0 34.0 2 32.24107882192262 34.0 31.0 34.0 30.0 34.0 3 32.328071379026724 34.0 31.0 34.0 30.0 34.0 4 35.926887287695536 37.0 35.0 37.0 35.0 37.0 5 35.84782505972889 37.0 35.0 37.0 35.0 37.0 6 35.90061036547386 37.0 35.0 37.0 35.0 37.0 7 35.82698773078221 37.0 35.0 37.0 35.0 37.0 8 35.821231015309046 37.0 35.0 37.0 33.0 37.0 9 37.44702505265366 39.0 37.0 39.0 34.0 39.0 10-11 37.365972409105325 39.0 37.0 39.0 34.0 39.0 12-13 37.29847730807474 39.0 37.0 39.0 34.0 39.0 14-15 38.439860996875524 40.0 38.0 41.0 33.5 41.0 16-17 38.409690799539604 40.0 38.0 41.0 33.5 41.0 18-19 38.60327014894636 40.0 38.0 41.0 34.0 41.0 20-21 38.61252898004152 40.0 38.0 41.0 34.0 41.0 22-23 38.53383504339268 40.0 38.0 41.0 34.0 41.0 24-25 38.43495823715434 40.0 38.0 41.0 34.0 41.0 26-27 38.241370675419745 40.0 38.0 41.0 33.5 41.0 28-29 38.096466803875714 40.0 37.0 41.0 33.0 41.0 30-31 37.88277546358591 40.0 37.0 41.0 33.0 41.0 32-33 37.69833820387546 40.0 37.0 41.0 33.0 41.0 34-35 37.607585411368134 40.0 36.0 41.0 33.0 41.0 36-37 37.4688361109953 40.0 36.0 41.0 32.5 41.0 38-39 37.341531889732444 40.0 36.0 41.0 32.0 41.0 40-41 37.2150537023069 40.0 36.0 41.0 31.5 41.0 42-43 36.98983326447612 40.0 36.0 41.0 31.0 41.0 44-45 36.70348720708837 39.5 35.5 41.0 30.5 41.0 46-47 36.34858008851626 39.0 35.0 41.0 30.0 41.0 48-49 35.99066763698478 39.0 35.0 41.0 29.0 41.0 50-51 35.74450033038826 39.0 35.0 41.0 28.0 41.0 52-53 35.56340068705598 39.0 35.0 40.0 28.0 41.0 54-55 35.32131136977838 38.5 35.0 40.0 27.0 41.0 56-57 35.03102920682811 38.0 34.5 40.0 26.5 41.0 58-59 34.732303633765554 38.0 34.0 40.0 26.0 41.0 60-61 34.30461734974283 37.5 34.0 40.0 25.5 41.0 62-63 33.999954389994606 37.0 33.0 40.0 25.0 41.0 64-65 33.618437813095 36.5 33.0 39.5 23.5 41.0 66-67 33.16324035714782 36.0 32.5 39.0 22.5 40.0 68-69 32.816648283686646 35.5 32.0 39.0 22.0 40.0 70-71 32.35352416256366 35.0 32.0 38.0 21.5 40.0 72-73 31.846809257946692 35.0 31.0 37.0 19.5 39.5 74-75 31.378640619689627 35.0 31.0 37.0 18.0 39.0 76-77 29.44409337795786 33.0 28.5 35.0 13.0 37.0 78-79 30.40250779223569 34.0 30.0 36.0 13.0 38.0 80-81 30.415411759800783 34.0 30.5 35.5 11.5 37.0 82-83 30.089101609237225 34.0 30.0 35.0 7.5 37.0 84-85 29.684834104660432 34.0 30.0 35.0 4.5 36.0 86-87 29.172725848491396 34.0 29.0 35.0 2.0 36.0 88-89 28.562726265424963 34.0 29.0 35.0 2.0 35.5 90-91 28.022494197674522 33.0 27.5 35.0 2.0 35.0 92-93 27.755412366249608 33.0 27.0 35.0 2.0 35.0 94-95 27.410618287211637 33.0 27.0 35.0 2.0 35.0 96-97 26.777864870567395 33.0 25.5 35.0 2.0 35.0 98-99 25.99236784153492 33.0 24.5 35.0 2.0 35.0 100-101 22.33999807957872 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11965722222516462 1101 2 0.1603106751547756 1101 3 0.10530967917221545 1101 4 0.10948744608632666 1101 5 -0.16979150763688722 1101 6 0.010435707243821923 1101 7 0.14763669948224134 1101 8 0.15153075155294005 1101 9 0.15042767620239061 1101 10-11 0.20874746176908587 1101 12-13 0.165621868595359 1101 14-15 0.2597680539579912 1101 16-17 0.38781068737812063 1101 18-19 0.2880605059534318 1101 20-21 0.27602735638473064 1101 22-23 0.1786456754712944 1101 24-25 0.1805383621212684 1101 26-27 0.17780098609517125 1101 28-29 0.27349905972589994 1101 30-31 0.2583644887992733 1101 32-33 0.354859530951785 1101 34-35 0.388876179551211 1101 36-37 0.3677276494072217 1101 38-39 0.36699011851089836 1101 40-41 0.5050276035961261 1101 42-43 0.4559132260456522 1101 44-45 0.4977116707092364 1101 46-47 0.4785570082891937 1101 48-49 0.4467959686827001 1101 50-51 0.46095533064508487 1101 52-53 0.4842841510399509 1101 54-55 0.5177806901828532 1101 56-57 0.49163630145082493 1101 58-59 0.5458589401621907 1101 60-61 0.48093379714587314 1101 62-63 0.5479611823957597 1101 64-65 0.559118503105644 1101 66-67 0.47722191417147286 1101 68-69 0.461716590917149 1101 70-71 0.6188143642683741 1101 72-73 0.45621685295524017 1101 74-75 0.4136299528396492 1101 76-77 0.5437073934135874 1101 78-79 0.330720853708133 1101 80-81 0.5530872990986673 1101 82-83 0.6625919483218787 1101 84-85 0.5870681660756567 1101 86-87 0.5415272122742643 1101 88-89 0.7714966438823296 1101 90-91 0.6782143402464484 1101 92-93 0.7422164131812963 1101 94-95 0.780969942446653 1101 96-97 0.5970986652618677 1101 98-99 0.6688597842824677 1101 100-101 1.1961362368896111 1102 1 0.26006309850908593 1102 2 0.21091499386390922 1102 3 0.18013617861429054 1102 4 0.08398066494002876 1102 5 -0.13269504822541478 1102 6 -0.04921742756330616 1102 7 0.03972539204932701 1102 8 0.15103520969853435 1102 9 0.24519343602339916 1102 10-11 0.203231136521282 1102 12-13 0.2331789248318259 1102 14-15 0.2860030809515237 1102 16-17 0.32347253741301074 1102 18-19 0.3138823142574054 1102 20-21 0.27676704669062957 1102 22-23 0.27514214519239744 1102 24-25 0.31860322149099574 1102 26-27 0.24269119494724833 1102 28-29 0.2952541100981918 1102 30-31 0.3036218089571392 1102 32-33 0.3502850173316645 1102 34-35 0.3563034870426023 1102 36-37 0.2843065217952869 1102 38-39 0.3706292640220923 1102 40-41 0.3715851835207573 1102 42-43 0.3816091965960169 1102 44-45 0.4038196007381387 1102 46-47 0.47021252328727314 1102 48-49 0.4096314516080781 1102 50-51 0.390810159770659 1102 52-53 0.2118243528265893 1102 54-55 0.18729011336419177 1102 56-57 0.2677992956751254 1102 58-59 0.27242456775321955 1102 60-61 0.2570328690996533 1102 62-63 0.16186422494195796 1102 64-65 0.19373043786158206 1102 66-67 0.3167844204066128 1102 68-69 0.29631550399491857 1102 70-71 0.33731934225526317 1102 72-73 0.24344375354963788 1102 74-75 0.2846461592189513 1102 76-77 0.19789884144728376 1102 78-79 0.23932787523294863 1102 80-81 0.4240402884682055 1102 82-83 0.39565748345964025 1102 84-85 0.5238634342632409 1102 86-87 0.5687227723628645 1102 88-89 0.40025882533848645 1102 90-91 0.4991395295192049 1102 92-93 0.5901720994679778 1102 94-95 0.6099419325668478 1102 96-97 0.6608221163617785 1102 98-99 0.8325920960220827 1102 100-101 1.3624623374105518 1103 1 0.2059258312887735 1103 2 0.21472570938991709 1103 3 0.17831691645265124 1103 4 0.0314916035895223 1103 5 0.020935317349561444 1103 6 0.0904536601277286 1103 7 0.09296154002052504 1103 8 0.10774838417292898 1103 9 0.1717127475410578 1103 10-11 0.2047245216300908 1103 12-13 0.15084063513245383 1103 14-15 0.21013450842478676 1103 16-17 0.13936486488574928 1103 18-19 0.030090184788782892 1103 20-21 0.1440075032879733 1103 22-23 0.19823932206372064 1103 24-25 0.2526176736985022 1103 26-27 0.20613149068989145 1103 28-29 0.0888320402678815 1103 30-31 0.1906866683580688 1103 32-33 0.1589128747535682 1103 34-35 0.10181847681957379 1103 36-37 0.18010953304175104 1103 38-39 0.08616384866613913 1103 40-41 0.2026995663978326 1103 42-43 0.17790586886074067 1103 44-45 0.17057529906268343 1103 46-47 0.21519744662656848 1103 48-49 0.24144475044348468 1103 50-51 0.2809943532865873 1103 52-53 0.35168534894039993 1103 54-55 0.30317245992612385 1103 56-57 0.1904950293433174 1103 58-59 0.30279210360190234 1103 60-61 0.44066649565192506 1103 62-63 0.2540020603743329 1103 64-65 0.32424995088897646 1103 66-67 0.31540042809778157 1103 68-69 0.2938866685824735 1103 70-71 0.2621883041825299 1103 72-73 0.3114833954827283 1103 74-75 0.4327102282174984 1103 76-77 0.5006560254962871 1103 78-79 0.35035693298705084 1103 80-81 0.4293997790596684 1103 82-83 0.4961093923671491 1103 84-85 0.5080113456867252 1103 86-87 0.5607712713927704 1103 88-89 0.3434109180258993 1103 90-91 0.5055643861862649 1103 92-93 0.4731652138374898 1103 94-95 0.60656144284523 1103 96-97 0.5983657631432067 1103 98-99 0.7922940583334608 1103 100-101 1.4329569177066332 1104 1 0.06494251489814218 1104 2 0.09397624832168816 1104 3 0.06117802563944963 1104 4 0.072693921782502 1104 5 -0.10379712356650117 1104 6 0.04883695116576092 1104 7 -0.027179509167233107 1104 8 0.044997052610071364 1104 9 0.08332175932370944 1104 10-11 0.09365846639257569 1104 12-13 -0.03294018959342537 1104 14-15 0.07761704920373091 1104 16-17 0.11721661119866411 1104 18-19 0.07774751854411477 1104 20-21 0.10719811175847838 1104 22-23 0.16776373017798818 1104 24-25 0.18667050934100615 1104 26-27 0.13802604633017523 1104 28-29 0.13909605206850273 1104 30-31 0.041723020337585126 1104 32-33 0.11361342069165659 1104 34-35 0.07177300288518751 1104 36-37 0.10950516862572357 1104 38-39 0.0939324148885774 1104 40-41 0.1285112324119808 1104 42-43 0.10687932887804408 1104 44-45 0.16287547228861143 1104 46-47 0.07967707261336443 1104 48-49 0.15933811107004914 1104 50-51 0.10034632655629849 1104 52-53 0.0709887097730828 1104 54-55 0.13238083803255307 1104 56-57 0.09900417881287638 1104 58-59 0.25900649327408587 1104 60-61 0.26628989563173633 1104 62-63 0.19641532748498491 1104 64-65 0.041480475389562343 1104 66-67 0.08527285764110815 1104 68-69 0.14800217594358145 1104 70-71 0.05837716350039557 1104 72-73 0.15642960883421964 1104 74-75 0.2199261154073966 1104 76-77 0.18921438371208055 1104 78-79 0.023198005302273117 1104 80-81 0.16875154683593863 1104 82-83 0.23120530265047634 1104 84-85 0.3092173337657087 1104 86-87 0.441306995325057 1104 88-89 0.4277112314412257 1104 90-91 0.4489890781651411 1104 92-93 0.5159984944212006 1104 94-95 0.5450007125669103 1104 96-97 0.7236286734107154 1104 98-99 0.9603585741981533 1104 100-101 1.5395206809043067 1105 1 0.09293644674902168 1105 2 0.1107055106183239 1105 3 0.06775760777250639 1105 4 0.059229375556050456 1105 5 0.002928783889437625 1105 6 0.0421032849971823 1105 7 0.08697562305158613 1105 8 0.11795535888734321 1105 9 0.17449426433583426 1105 10-11 0.12799737168485592 1105 12-13 0.12423443007671864 1105 14-15 0.1614478799535064 1105 16-17 0.17802959466836654 1105 18-19 0.18143565681709362 1105 20-21 0.10583621844847357 1105 22-23 0.040735979122310084 1105 24-25 0.07742953129743313 1105 26-27 0.17240828775744177 1105 28-29 0.19472535543604153 1105 30-31 0.13308437520141325 1105 32-33 0.1799460831578017 1105 34-35 0.16536188354159265 1105 36-37 0.17965026018610075 1105 38-39 0.23006308614562698 1105 40-41 0.222534534351702 1105 42-43 0.3040508995740012 1105 44-45 0.23335683529757745 1105 46-47 0.1601780165295068 1105 48-49 0.22034890839980648 1105 50-51 0.1620846621858547 1105 52-53 0.2366370125986137 1105 54-55 0.31111277729703346 1105 56-57 0.28510253421412557 1105 58-59 0.267657821270717 1105 60-61 0.31728126347339725 1105 62-63 0.29075442901637416 1105 64-65 0.2831022316677476 1105 66-67 0.2094050522950326 1105 68-69 0.2087577110218959 1105 70-71 0.2970311062013664 1105 72-73 0.36339714476943996 1105 74-75 0.3937530764916666 1105 76-77 0.37161529765330314 1105 78-79 0.13991987229201897 1105 80-81 0.31088122926595574 1105 82-83 0.4711679602875627 1105 84-85 0.538548738060598 1105 86-87 0.511585365942107 1105 88-89 0.41950377327725263 1105 90-91 0.4831760798563707 1105 92-93 0.5072643704825168 1105 94-95 0.6275477519578523 1105 96-97 0.7756659835516544 1105 98-99 0.8925661714253934 1105 100-101 1.6032561430846393 1106 1 0.19836146768174245 1106 2 0.12392368217249583 1106 3 0.10310859405119288 1106 4 0.08022131693218881 1106 5 0.07815898552843947 1106 6 0.11285975694558914 1106 7 0.09865586259244452 1106 8 0.08810764534080562 1106 9 0.09492550315362536 1106 10-11 0.0887788230065567 1106 12-13 0.15616658503801517 1106 14-15 0.15161339198110824 1106 16-17 0.23878793475098092 1106 18-19 0.04998679779244952 1106 20-21 0.08293216448066687 1106 22-23 0.0901717938488602 1106 24-25 0.11247645760463598 1106 26-27 0.14185726977002133 1106 28-29 0.19246147404769687 1106 30-31 0.11540949408731649 1106 32-33 0.1320628599064264 1106 34-35 0.10847238819720673 1106 36-37 0.05852416870465049 1106 38-39 0.20373763618320595 1106 40-41 0.28409360746569945 1106 42-43 0.11818375743171572 1106 44-45 0.0790181154620413 1106 46-47 0.19836492379398152 1106 48-49 0.27393512862223446 1106 50-51 0.2825698175134974 1106 52-53 0.11820295788179891 1106 54-55 0.16975173223769247 1106 56-57 0.13997680528476764 1106 58-59 0.15460122438173585 1106 60-61 0.27607406990350825 1106 62-63 0.2648612437426294 1106 64-65 0.3065070971518793 1106 66-67 0.1905019239425556 1106 68-69 0.23428102903807968 1106 70-71 0.23154006231653312 1106 72-73 0.2998709059356237 1106 74-75 0.4254384395581603 1106 76-77 0.35776822644183426 1106 78-79 0.30161930319597374 1106 80-81 0.23732229371192304 1106 82-83 0.3937369450047079 1106 84-85 0.42891595612315214 1106 86-87 0.6056200141485348 1106 88-89 0.7173156955873488 1106 90-91 0.5887960745463232 1106 92-93 0.6660644629274763 1106 94-95 0.9270872466756366 1106 96-97 0.9728971230464865 1106 98-99 1.187886568099355 1106 100-101 1.5407156437711294 1107 1 0.023646125675746532 1107 2 0.00446734927027137 1107 3 -0.02639935292620521 1107 4 -0.00643461505922005 1107 5 -0.01210909020702644 1107 6 -0.08855422554445624 1107 7 -0.031079758548003156 1107 8 0.0026904279392567787 1107 9 0.008163365778990794 1107 10-11 -0.10502708172257513 1107 12-13 0.03320155707785233 1107 14-15 -0.03154992084916586 1107 16-17 0.009766414232309728 1107 18-19 0.08964673163752224 1107 20-21 -0.05252604065415767 1107 22-23 0.011564546869401227 1107 24-25 0.036007479007345466 1107 26-27 -0.09482815888041074 1107 28-29 -0.006297344843972041 1107 30-31 -0.010597882834133543 1107 32-33 -0.06300024379209646 1107 34-35 -0.0365676027724291 1107 36-37 -0.07009786287665065 1107 38-39 -0.12833406812011816 1107 40-41 -0.25678057890966244 1107 42-43 -0.11217664734427046 1107 44-45 -0.07905643897590409 1107 46-47 -0.2186157790360994 1107 48-49 -0.24661172463704162 1107 50-51 -0.37731083781434904 1107 52-53 -0.36938354799881523 1107 54-55 -0.2651019437308335 1107 56-57 -0.25823122552523614 1107 58-59 -0.28487015138132676 1107 60-61 -0.3413454991057634 1107 62-63 -0.35453827218135814 1107 64-65 -0.39093204730862396 1107 66-67 -0.25464604250002765 1107 68-69 -0.28453493763548465 1107 70-71 -0.26777070537138314 1107 72-73 -0.22872428412122758 1107 74-75 -0.34548014705131536 1107 76-77 -0.4173402786071314 1107 78-79 -0.2991630184246894 1107 80-81 -0.19530240403074828 1107 82-83 -0.2754894068142626 1107 84-85 -0.2077307046606265 1107 86-87 -0.21025229332011364 1107 88-89 -0.19986325729141186 1107 90-91 0.009008322812725567 1107 92-93 0.03473108916258383 1107 94-95 0.17163570404244766 1107 96-97 0.2468156635175589 1107 98-99 0.45999680232284845 1107 100-101 1.1467511854316434 1108 1 0.11794337791421583 1108 2 0.08367103860508252 1108 3 0.05464141191165339 1108 4 -0.003642848381815611 1108 5 0.006511031552378199 1108 6 0.017781514468268256 1108 7 0.028835240033743048 1108 8 0.009136383610155008 1108 9 0.07569257305515009 1108 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-2.0572566805513155 2316 100-101 -2.083757185425778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 42.0 5 113.0 6 346.0 7 729.0 8 1462.0 9 2813.0 10 4782.0 11 7235.0 12 10293.0 13 14782.0 14 17482.0 15 23491.0 16 30530.0 17 41870.0 18 39505.0 19 26065.0 20 23746.0 21 24766.0 22 26321.0 23 28885.0 24 33464.0 25 39678.0 26 46959.0 27 57641.0 28 70556.0 29 85605.0 30 103268.0 31 125869.0 32 154439.0 33 193645.0 34 259135.0 35 369395.0 36 540144.0 37 818467.0 38 670693.0 39 63226.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.630192618010796 19.750811577089884 14.76767632251543 20.851319482383897 2 15.012463786792807 23.15181561934218 38.509475131176146 23.32624546268887 3 21.63766451503677 26.230907917063067 30.00754270726336 22.123884860636796 4 12.4603249807642 17.321239733010906 38.62204719435295 31.59638809187194 5 12.356490710837488 39.76556747669589 33.593754936775156 14.284186875691466 6 29.11046179450098 37.615731644851834 17.41791104744293 15.855895513204254 7 26.97757276438326 34.44424650628622 21.150761914508404 17.427418814822115 8 25.65253261873447 32.79030894777338 19.76255001623514 21.794608417257006 9 25.725761314376754 18.6817065975315 21.915544420481293 33.676987667610454 10-11 25.012716473803305 26.705631003685443 26.81247970607447 21.469172816436785 12-13 27.8145479492554 24.685133033393853 26.867375959105132 20.63294305824562 14-15 23.137551437599573 25.94727938212972 28.23648471938476 22.678684460885947 16-17 20.681841699589537 29.388969477217803 28.272125160350136 21.65706366284252 18-19 20.340394671841697 29.194673863192726 30.152420804732323 20.312510660233254 20-21 22.442182053410452 26.327863922991106 29.46422773164134 21.7657262919571 22-23 21.258570827537326 26.774033377427216 29.94449729814414 22.02289849689132 24-25 22.597964877143788 26.786771739337805 29.83565307117472 20.77961031234369 26-27 21.848806278242883 25.836600829885587 31.758007061683983 20.556585830187547 28-29 20.283426385324947 28.614997226760057 30.46168443688068 20.63989195103431 30-31 21.07165824587204 26.728751865145995 30.12686151362046 22.0727283753615 32-33 21.28684650401204 27.077409402230973 30.393180988334706 21.242563105422285 34-35 22.629568685810774 26.422594885040457 30.117091734009165 20.830744695139604 36-37 21.961157660482655 25.936816720521342 31.309845694382858 20.792179924613144 38-39 21.20192891501121 27.302249630048674 31.008472583419582 20.487348871520528 40-41 21.148662718352583 27.566998017756294 30.317108451272283 20.967230812618837 42-43 20.619993619124934 27.798275900193005 28.998853337797843 22.582877142884218 44-45 21.537515286570045 26.559179605052773 29.175196759114485 22.7281083492627 46-47 22.523963417245778 27.65710698978645 29.022838889347234 20.796090703620536 48-49 22.154478426964815 27.45008457585977 29.03452372950482 21.360913267670593 50-51 20.35126043519048 27.933353758879587 30.33923884307179 21.376146962858144 52-53 21.564296841538713 28.85293995019539 28.05940166242784 21.523361545838053 54-55 20.618994735266135 27.427570427028414 28.900836773035266 23.05259806467019 56-57 22.018889357910158 27.223842037229616 28.47726172663029 22.280006878229937 58-59 23.409085424611337 26.850317824729746 28.106316481439997 21.634280269218916 60-61 21.744372716128964 27.49178684535769 30.035508422131763 20.728332016381582 62-63 21.07173021902458 27.639890257130094 29.632937516938064 21.655442006907254 64-65 20.638492202522286 29.093244645507916 28.596163416827935 21.672099735141863 66-67 21.36811078838321 27.730250240192785 27.736997370388135 23.164641601035875 68-69 20.702686448740415 27.873936658787024 28.433816682691276 22.98956020978129 70-71 22.14113634388083 27.692469217660605 28.021063489143554 22.145330949315014 72-73 21.752472858256485 26.70741244710945 29.492591848569727 22.04752284606434 74-75 20.52587411739229 29.3584579359077 28.060753278185015 22.054914668514996 76-77 21.149930298405252 27.490910727471757 28.12150567580965 23.237653298313344 78-79 21.35362807959776 27.669417020545982 28.525420539959757 22.451534359896495 80-81 21.998566999470995 27.601280679471184 28.074390693658422 22.325761627399395 82-83 21.468343798243886 27.248911320385456 28.990899779313935 22.291845102056726 84-85 20.83990635419391 28.04220631136346 28.581503563518567 22.536383770924065 86-87 20.904015398131186 28.278015798312612 28.117766157957323 22.70020264559888 88-89 20.59570194869168 28.183021568868604 27.354082692285747 23.867193790153966 90-91 21.145680875160593 27.14898402370474 27.790651260091177 23.91468384104349 92-93 21.85572504318121 28.104760880774126 27.832350651006248 22.207163425038416 94-95 21.259465676939936 27.532232828362464 28.109952454303976 23.098349040393625 96-97 19.945449879929345 27.610704488218012 29.229229393640345 23.214616238212297 98-99 20.77766954350828 28.296700033986404 27.30803683671239 23.617593585792925 100-101 20.45362859285305 26.934222969622272 28.34452222374538 24.267626213779298 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6586.0 1 4284.0 2 2661.0 3 3358.0 4 2792.0 5 2530.5 6 4207.5 7 8559.5 8 12611.0 9 13008.5 10 10192.0 11 7449.5 12 6887.5 13 7028.0 14 7410.5 15 7600.0 16 7615.0 17 7902.5 18 8323.5 19 8805.5 20 9636.0 21 10837.0 22 12309.5 23 14302.0 24 16573.5 25 19678.0 26 24164.5 27 29075.0 28 33627.0 29 39161.0 30 45677.5 31 52489.5 32 61588.0 33 70267.5 34 78191.5 35 84994.0 36 94738.5 37 104817.5 38 107522.5 39 107921.0 40 107737.0 41 109635.0 42 112517.0 43 116880.5 44 122323.5 45 126807.5 46 133220.0 47 176328.5 48 270777.0 49 264773.0 50 174904.0 51 133402.0 52 115451.5 53 108935.5 54 104796.5 55 96932.5 56 86759.5 57 76882.0 58 69255.0 59 61352.0 60 52041.0 61 42648.5 62 34467.0 63 26903.5 64 20009.0 65 15029.0 66 11084.5 67 8739.0 68 6442.5 69 4192.5 70 2982.0 71 2220.0 72 1722.0 73 1434.5 74 1183.5 75 922.5 76 606.5 77 354.5 78 254.5 79 177.5 80 101.5 81 62.0 82 42.5 83 31.0 84 19.0 85 10.0 86 6.0 87 5.5 88 6.5 89 5.0 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23469569535043266 2 0.0 3 0.0 4 0.0 5 0.030019216847148363 6 0.003588155549069922 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0027669227649518065 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.411274641721405E-4 26-27 0.0013139724545889857 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006784646231868128 36-37 0.017043738858082132 38-39 0.013278702401663692 40-41 0.008414477449579466 42-43 0.008275499593805632 44-45 0.00382820820904291 46-47 6.696205778193868E-4 48-49 0.001794077774534961 50-51 6.317175262447046E-5 52-53 0.0 54-55 0.0 56-57 0.0013013381040640915 58-59 4.1693356732150507E-4 60-61 0.0023752578986800895 62-63 8.212327841181161E-4 64-65 0.0016045625166615496 66-67 0.0057359951383019175 68-69 2.5268701049788184E-4 70-71 2.5268701049788187E-5 72-73 0.0 74-75 4.04299216796611E-4 76-77 0.06040482985951866 78-79 0.1432861693028239 80-81 8.717701862176924E-4 82-83 8.465014851679043E-4 84-85 0.0 86-87 0.1075941290699981 88-89 0.35502524974952404 90-91 0.39086890218864856 92-93 3.663961652219287E-4 94-95 2.2741830944809365E-4 96-97 8.844045367425864E-5 98-99 0.0 100-101 0.0010739197946159978 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3957465.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.77585264561265 #Duplication Level Percentage of deduplicated Percentage of total 1 68.81463581558268 26.683461782465322 2 15.553545703474626 12.062039926294675 3 6.07235854086867 7.0638263997615285 4 3.0120056446580694 4.671723481800594 5 1.7251849650414361 3.3447758995436567 6 1.0613418690616316 2.4692661552811774 7 0.7416974257202978 2.0131965061152353 8 0.5095954780658436 1.580799933308135 9 0.37060050932628974 1.293331566602269 >10 1.858300987070298 13.04285301274736 >50 0.1618871095398865 4.389043906380016 >100 0.10663870953011498 7.777332012334402 >500 0.00776824495217339 2.040932210055347 >1k 0.002872292246434787 2.3838677460662656 >5k 5.875143231343882E-4 1.3617689775940298 >10k+ 9.791905385573138E-4 7.8217804836499205 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 65260 1.649035430509177 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 32737 0.8272214662669157 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26079 0.6589824546774261 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 22313 0.5638205265239238 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 21484 0.5428727733536494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20377 0.5149003212915338 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17840 0.45079362672822126 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15205 0.38421059946202935 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 14528 0.3671036888513227 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 14523 0.3669773453460738 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 13826 0.3493650607143714 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12705 0.3210388468375589 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11574 0.2924599459502485 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10298 0.26021708341071875 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 10258 0.2592063353687272 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7578 0.19148621655529488 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7204 0.1820357223626741 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6211 0.1569439022202344 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5948 0.15029823384414012 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5921 0.14961597891579587 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5400 0.1364509856688562 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5323 0.1345052956880225 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5166 0.13053810962320578 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5047 0.127531134198281 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4957 0.12525695110380003 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4775 0.12065804751273859 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4646 0.11739838507731591 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4632 0.11704462326261889 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4604 0.1163370996332248 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.5268701049788184E-5 0.0 3 0.0 0.0 0.0 2.5268701049788184E-5 0.0 4 0.0 0.0 0.0 2.5268701049788184E-5 0.0 5 0.0 0.0 0.0 2.5268701049788184E-5 0.0 6 0.0 0.0 0.0 2.5268701049788184E-5 0.0 7 0.0 0.0 0.0 2.5268701049788184E-5 0.0 8 0.0 0.0 0.0 2.5268701049788184E-5 0.0 9 0.0 0.0 0.0 2.5268701049788184E-5 0.0 10-11 0.0 0.0 0.0 5.053740209957637E-5 0.0 12-13 1.2634350524894092E-5 0.0 0.0 6.317175262447046E-5 2.5268701049788184E-5 14-15 3.7903051574682275E-5 0.0 0.0 1.0107480419915273E-4 2.5268701049788184E-5 16-17 5.053740209957637E-5 0.0 0.0 2.1478395892319955E-4 2.5268701049788184E-5 18-19 6.317175262447046E-5 0.0 0.0 3.411274641721405E-4 2.5268701049788184E-5 20-21 7.580610314936455E-5 0.0 0.0 6.569862272944928E-4 2.5268701049788184E-5 22-23 7.580610314936455E-5 0.0 0.0 0.001642465568236232 2.5268701049788184E-5 24-25 1.0107480419915273E-4 0.0 0.0 0.002842728868101171 2.5268701049788184E-5 26-27 1.0107480419915273E-4 0.0 0.0 0.004510463137387191 2.5268701049788184E-5 28-29 1.516122062987291E-4 0.0 0.0 0.00870506751165203 2.5268701049788184E-5 30-31 1.7688090734851728E-4 0.0 0.0 0.020657163108201843 2.5268701049788184E-5 32-33 1.7688090734851728E-4 0.0 0.0 0.045875326755890455 2.5268701049788184E-5 34-35 1.895152578734114E-4 0.0 0.0 0.08304558600012887 2.5268701049788184E-5 36-37 2.0214960839830547E-4 0.0 0.0 0.13847248175283924 2.5268701049788184E-5 38-39 2.0214960839830547E-4 0.0 0.0 0.220936887628823 2.5268701049788184E-5 40-41 2.0214960839830547E-4 0.0 0.0 0.3154923669571304 2.5268701049788184E-5 42-43 2.4005265997298776E-4 0.0 0.0 0.4132822400198106 2.5268701049788184E-5 44-45 2.7795571154767005E-4 0.0 0.0 0.53205776930434 2.5268701049788184E-5 46-47 3.790305157468228E-4 0.0 0.0 0.6776939278047942 2.5268701049788184E-5 48-49 3.790305157468228E-4 0.0 0.0 0.8257053442039285 2.5268701049788184E-5 50-51 3.790305157468228E-4 0.0 0.0 0.9919733971115348 2.5268701049788184E-5 52-53 3.790305157468228E-4 0.0 0.0 1.1620443895271342 2.5268701049788184E-5 54-55 3.790305157468228E-4 0.0 0.0 1.3458615553138182 2.5268701049788184E-5 56-57 4.2956791784639915E-4 0.0 0.0 1.5880115174739384 2.5268701049788184E-5 58-59 4.2956791784639915E-4 0.0 0.0 1.8375525746911217 2.5268701049788184E-5 60-61 4.2956791784639915E-4 0.0 0.0 2.1072201522944614 2.5268701049788184E-5 62-63 5.306427220455518E-4 0.0 0.0 2.3796799213638025 2.5268701049788184E-5 64-65 5.559114230953401E-4 0.0 0.0 2.648589437935648 2.5268701049788184E-5 66-67 5.559114230953401E-4 0.0 0.0 2.9656484643578653 2.5268701049788184E-5 68-69 5.559114230953401E-4 0.0 0.0 3.3288986762990955 2.5268701049788184E-5 70-71 5.685457736202342E-4 0.0 0.0 3.724745512594552 2.5268701049788184E-5 72-73 5.811801241451282E-4 0.0 0.0 4.1073894525914945 5.053740209957637E-5 74-75 5.938144746700223E-4 0.0 0.0 4.5117897441923045 5.053740209957637E-5 76-77 6.064488251949164E-4 0.0 0.0 4.922823069818685 5.053740209957637E-5 78-79 6.064488251949164E-4 0.0 0.0 5.438822579605884 5.053740209957637E-5 80-81 6.82254928344281E-4 0.0 0.0 5.998233717796619 5.053740209957637E-5 82-83 7.075236293940691E-4 0.0 0.0 6.525427262148876 5.053740209957637E-5 84-85 7.327923304438573E-4 0.0 0.0 7.065836337150171 5.053740209957637E-5 86-87 7.706953820185397E-4 0.0 0.0 7.615102091869416 5.053740209957637E-5 88-89 7.833297325434337E-4 0.0 0.0 8.212492087737983 5.053740209957637E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 170 1.13239366E-7 25.128355 2 GTAAGCG 515 0.0 24.413385 94-95 TACCTGG 3005 0.0 23.376944 2 GTACATG 28150 0.0 22.950203 1 TACATGG 28405 0.0 22.47491 2 ACATGGG 28185 0.0 21.286266 3 GAGTACT 20365 0.0 21.271208 12-13 AGTACTT 20970 0.0 20.725399 12-13 CATGGGG 7905 0.0 19.994606 4 GTACTTT 21900 0.0 19.693623 14-15 CATGGGA 19415 0.0 19.020096 4 ACTTTTT 24535 0.0 17.761951 16-17 TACTTTT 24515 0.0 17.699364 14-15 GTATAGG 1160 0.0 17.635008 1 ATGGGAT 7495 0.0 17.54198 5 GTGTAGC 1335 0.0 17.105082 1 ATGGGAG 6095 0.0 16.82095 5 AGAGTAC 43170 0.0 16.80472 10-11 GTACCTG 4395 0.0 16.669672 1 AGTACAT 22145 0.0 16.096622 2 >>END_MODULE