FastQCFastQC Report
Thu 26 May 2016
SRR938791_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938791_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3871740
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138780.35844349052364055No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT127440.3291543337104248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102810.2655395248647895No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74270.19182589740013534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70640.18245026783823295No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC51630.13335089649614904No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51260.13239525381353087No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG174300.024.0426251
TACATGG173300.023.4315682
ACATGGG179500.021.7247373
GAGTACT125200.021.00798812-13
TACCTGG30900.020.0877822
AGTACTT135200.019.54169712-13
GTACTTT136250.019.28709614-15
CATGGGG73900.019.0428144
GTATCAA348100.018.1940521
GTGTAGC26250.017.5173911
CATGGGA105900.017.404954
GTACCTG36800.017.2617171
AGAGTAC254850.017.25980410-11
ACTTTTT164600.016.33997316-17
TCAACGC385450.016.2633864
CAACGCA386050.016.2626555
TATCAAC387000.016.234982
TACTTTT162650.016.20024114-15
ATCAACG387000.016.1860053
CGTATAC2053.3980302E-416.1794343
Produced by FastQC (version 0.11.5)