FastQCFastQC Report
Thu 26 May 2016
SRR938791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3871740
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT141340.36505550476013365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133160.34392805302008916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110960.2865894920630001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76570.19776637894073465No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68810.17772371078636479No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47710.12322624969651888No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47480.12263220154245895No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39300.1015047498024144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG174150.021.2511841
TACATGG173800.021.0506882
GAGTACT132400.020.59181412-13
AGTACTT141100.019.67530312-13
ACATGGG177100.019.6402553
GTACTTT140300.019.3140714-15
TACCTGG29600.018.2757362
CATGGGG75250.017.719914
TAGCCCT30200.017.4412564
GGACCGA7200.017.1357296
CGCTATA1700.001756056416.8107831
CCGATCG2858.683928E-716.6503169
GTATCAA358450.016.6364771
CCTATAC10350.016.5053163
AGAGTAC273100.016.45565610-11
TACTTTT167450.016.3809114-15
CATGGGA101450.016.1839964
GTATAGG12700.016.126881
ACTTTTT170350.016.03188316-17
GTATACG2406.010633E-515.876851
Produced by FastQC (version 0.11.5)