##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938791_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3871740 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99019097356744 33.0 31.0 34.0 30.0 34.0 2 32.21068279378264 34.0 31.0 34.0 30.0 34.0 3 32.30742818474381 34.0 31.0 34.0 30.0 34.0 4 35.932214456549254 37.0 35.0 37.0 35.0 37.0 5 35.84123649831859 37.0 35.0 37.0 35.0 37.0 6 35.86952248859686 37.0 35.0 37.0 35.0 37.0 7 35.80570621994246 37.0 35.0 37.0 33.0 37.0 8 35.77149601987737 37.0 35.0 37.0 33.0 37.0 9 37.42265983769571 39.0 37.0 39.0 34.0 39.0 10-11 37.34646399293341 39.0 37.0 39.0 34.0 39.0 12-13 37.2792462820334 39.0 37.0 39.0 33.0 39.0 14-15 38.48940618429957 40.0 38.0 41.0 34.0 41.0 16-17 38.42656893283123 40.0 38.0 41.0 33.0 41.0 18-19 38.45842670220624 40.0 38.0 41.0 33.5 41.0 20-21 38.53075103183582 40.0 38.0 41.0 34.0 41.0 22-23 38.489605965276596 40.0 38.0 41.0 34.0 41.0 24-25 38.42753180740442 40.0 38.0 41.0 34.0 41.0 26-27 38.264376869314574 40.0 38.0 41.0 33.5 41.0 28-29 38.11418561163715 40.0 38.0 41.0 33.0 41.0 30-31 37.910277033065235 40.0 37.0 41.0 33.0 41.0 32-33 37.70170349765222 40.0 37.0 41.0 32.5 41.0 34-35 37.67538096566402 40.0 37.0 41.0 33.0 41.0 36-37 37.60428373289529 40.0 37.0 41.0 32.0 41.0 38-39 37.513871540960906 40.0 37.0 41.0 32.0 41.0 40-41 37.380316730978834 40.0 37.0 41.0 31.5 41.0 42-43 37.219145526300835 40.0 36.0 41.0 31.0 41.0 44-45 36.98708242288997 40.0 36.0 41.0 31.0 41.0 46-47 36.727123593009864 40.0 36.0 41.0 30.0 41.0 48-49 36.47299870342533 39.0 35.5 41.0 30.0 41.0 50-51 36.25310286847774 39.0 35.0 41.0 30.0 41.0 52-53 36.06404898056171 39.0 35.0 41.0 29.0 41.0 54-55 35.84478580689819 39.0 35.0 40.0 28.5 41.0 56-57 35.59731787258442 38.5 35.0 40.0 28.0 41.0 58-59 35.31743854184424 38.0 34.5 40.0 28.0 41.0 60-61 34.97225743464179 38.0 34.0 40.0 27.0 41.0 62-63 34.618260781974 37.5 34.0 40.0 26.0 41.0 64-65 34.26955851890881 37.0 33.5 40.0 26.0 41.0 66-67 33.87744941550827 36.5 33.0 39.0 26.0 41.0 68-69 33.434630941127246 36.0 33.0 39.0 25.0 40.0 70-71 32.96280251256541 35.0 32.0 38.5 24.0 40.0 72-73 32.60188119553482 35.0 32.0 38.0 23.5 40.0 74-75 32.17731872491438 35.0 31.5 37.0 22.0 39.0 76-77 30.110005578887012 33.0 29.0 35.5 20.0 37.5 78-79 31.189151905861447 34.0 31.0 36.0 20.0 38.5 80-81 31.236338313006556 35.0 31.0 36.0 20.0 37.5 82-83 30.91994322965902 34.0 31.0 35.5 20.0 37.0 84-85 30.51456786871019 34.0 31.0 35.0 18.0 37.0 86-87 29.984839761967486 34.0 30.0 35.0 13.0 36.0 88-89 29.461388936240553 34.0 29.5 35.0 7.0 36.0 90-91 29.035674528764844 34.0 29.0 35.0 2.0 35.0 92-93 28.748456766208477 34.0 29.0 35.0 2.0 35.0 94-95 28.356378010920153 34.0 29.0 35.0 2.0 35.0 96-97 27.83201635440396 33.5 28.5 35.0 2.0 35.0 98-99 27.099909730508763 33.0 27.0 35.0 2.0 35.0 100-101 23.921398260213753 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12857106706791654 1101 2 0.14804749962366515 1101 3 0.14606090079715983 1101 4 0.15693629489366145 1101 5 0.12523986762851536 1101 6 0.18127063344482508 1101 7 0.17982533229989883 1101 8 0.10505979429387224 1101 9 0.21744581819358189 1101 10-11 0.2619554088732272 1101 12-13 0.2572397372181854 1101 14-15 0.3436771939503842 1101 16-17 0.2524533824657098 1101 18-19 0.31223209861249757 1101 20-21 0.32467331657736054 1101 22-23 0.22253500817702587 1101 24-25 0.26794482467587954 1101 26-27 0.23454938018734595 1101 28-29 0.3387587086352397 1101 30-31 0.23622703107960064 1101 32-33 0.25770723098541026 1101 34-35 0.27290724184616977 1101 36-37 0.26432635737630505 1101 38-39 0.3670664165602062 1101 40-41 0.35329883600367396 1101 42-43 0.33720034972702706 1101 44-45 0.37982965638079946 1101 46-47 0.36981842326774483 1101 48-49 0.313799323773992 1101 50-51 0.3939392003584814 1101 52-53 0.45660015693316325 1101 54-55 0.40591217385907896 1101 56-57 0.2705238204420155 1101 58-59 0.2171132889752485 1101 60-61 0.22985247407109455 1101 62-63 0.27061385909841107 1101 64-65 0.358255896543902 1101 66-67 0.28485111016436804 1101 68-69 0.3363319098117401 1101 70-71 0.2781613002152099 1101 72-73 0.26701669679333406 1101 74-75 0.3935324129266746 1101 76-77 0.2655905097039053 1101 78-79 0.24082797042499138 1101 80-81 0.2783577511255011 1101 82-83 0.3204215210855459 1101 84-85 0.3020310388937517 1101 86-87 0.4874605152375793 1101 88-89 0.5275995779023894 1101 90-91 0.6353061304740315 1101 92-93 0.5703804056035509 1101 94-95 0.6706078464407774 1101 96-97 0.6302612059593891 1101 98-99 0.647840419908448 1101 100-101 1.1369907947008322 1102 1 0.03612045303383127 1102 2 0.07015616019386073 1102 3 0.10833543068626028 1102 4 -0.01865241672205542 1102 5 0.07144879372696522 1102 6 0.06752368072487513 1102 7 0.0668054348487388 1102 8 0.06453325863640202 1102 9 0.10235734692670917 1102 10-11 0.1535503523912496 1102 12-13 0.12371050049745236 1102 14-15 0.16661899233203314 1102 16-17 0.13617425447422704 1102 18-19 0.1489418802384037 1102 20-21 0.1541867246095876 1102 22-23 0.14384987375334646 1102 24-25 0.05632975194173895 1102 26-27 0.11899323011810026 1102 28-29 0.15417594279225 1102 30-31 0.1836123074235516 1102 32-33 0.22831814625671853 1102 34-35 0.15160804186447763 1102 36-37 0.1963138563748501 1102 38-39 0.1512424492018667 1102 40-41 0.1836526066193116 1102 42-43 0.2122592208885976 1102 44-45 0.1013614081805514 1102 46-47 0.09579247989851325 1102 48-49 0.19020672365724778 1102 50-51 0.24599303850070697 1102 52-53 0.20668370677121572 1102 54-55 0.12758105326118852 1102 56-57 0.08412191424673665 1102 58-59 0.00621976351543907 1102 60-61 0.04371001587462331 1102 62-63 -0.029840157016408853 1102 64-65 0.06805247271731929 1102 66-67 0.04514290511246344 1102 68-69 0.11934983296877988 1102 70-71 -0.0022081359950547608 1102 72-73 0.1851661107999547 1102 74-75 0.16249608019230521 1102 76-77 0.06481037739758477 1102 78-79 0.04905448204756979 1102 80-81 0.1014458332561361 1102 82-83 0.21240152348903152 1102 84-85 0.2436183284454465 1102 86-87 0.21601259940545603 1102 88-89 0.37322258214855353 1102 90-91 0.4235843314056673 1102 92-93 0.4529109070170314 1102 94-95 0.503379412027396 1102 96-97 0.6929092064858686 1102 98-99 0.7048245568844003 1102 100-101 0.8746737030363576 1103 1 0.08033085084802849 1103 2 0.07129704637056733 1103 3 0.02663907519944786 1103 4 0.03910226452416765 1103 5 0.04376078178181331 1103 6 0.008475552941106912 1103 7 0.06734320659247572 1103 8 0.10389510189298079 1103 9 0.0318062949517568 1103 10-11 0.03674881352151971 1103 12-13 0.06882885120846538 1103 14-15 0.09148714263997704 1103 16-17 -1.5363457586659024E-4 1103 18-19 0.08123087133598972 1103 20-21 0.1418756256669269 1103 22-23 0.017449275084480576 1103 24-25 0.04211881078121138 1103 26-27 0.08960094535381558 1103 28-29 0.10874259233527539 1103 30-31 0.03951640044444815 1103 32-33 0.15571825520824234 1103 34-35 -0.10567830306588633 1103 36-37 -0.023394126723552233 1103 38-39 0.16933552936873753 1103 40-41 0.11283552663308427 1103 42-43 0.17373832957479607 1103 44-45 0.04931966083519512 1103 46-47 0.06642725660434223 1103 48-49 0.09752295263609767 1103 50-51 0.14654090096210837 1103 52-53 0.06268817773535318 1103 54-55 0.0036564556197333786 1103 56-57 -0.0319400738081157 1103 58-59 -0.17147622221360592 1103 60-61 -0.10498863436840367 1103 62-63 -0.08637777775219746 1103 64-65 -0.006454387559408303 1103 66-67 -0.0778663303639533 1103 68-69 -0.08141804947647557 1103 70-71 -0.18060408587735566 1103 72-73 -0.10797647033506053 1103 74-75 -0.09229828086063208 1103 76-77 -0.11171430887283051 1103 78-79 -0.2247210834560036 1103 80-81 -0.162886796314325 1103 82-83 -0.21713368958740986 1103 84-85 -0.2975073087516229 1103 86-87 -0.09255219711486617 1103 88-89 0.03541185155236448 1103 90-91 0.06103941712737182 1103 92-93 0.02876932366346807 1103 94-95 0.04315377511258944 1103 96-97 0.19873257376541176 1103 98-99 0.4941226098698408 1103 100-101 0.9811554367204458 1104 1 -0.010145414066499825 1104 2 0.027587158070723206 1104 3 -0.009360621172618266 1104 4 -0.054851427428111776 1104 5 -0.06295345138303077 1104 6 0.02888192709971804 1104 7 -0.0449480773706199 1104 8 -0.05191996447422298 1104 9 -0.045297032618847766 1104 10-11 0.017085384238384904 1104 12-13 -0.05819535847169277 1104 14-15 -0.03733168978388335 1104 16-17 0.10625686503573206 1104 18-19 0.07594051374790922 1104 20-21 0.0024644948584935378 1104 22-23 6.046847033474023E-4 1104 24-25 8.482616765732587E-4 1104 26-27 0.05719397999386899 1104 28-29 0.04551510347346266 1104 30-31 0.011113493221081683 1104 32-33 -0.03645632634570717 1104 34-35 0.00528232022197983 1104 36-37 0.030778497021728413 1104 38-39 0.1518776978805505 1104 40-41 0.17361850082358643 1104 42-43 0.14306595847725134 1104 44-45 0.11396276563640129 1104 46-47 0.020334818517113717 1104 48-49 -0.07760706095674408 1104 50-51 -0.0948503458783918 1104 52-53 -0.19005647934253034 1104 54-55 -0.1146067996547444 1104 56-57 -0.1456345455264909 1104 58-59 -0.09366786805162519 1104 60-61 -0.11151709258632536 1104 62-63 -0.25143494176592185 1104 64-65 -0.11518740847706255 1104 66-67 -0.13855237880134297 1104 68-69 -0.03845723212585739 1104 70-71 -0.1758780664282682 1104 72-73 -0.26959430163172726 1104 74-75 -0.11363212146103763 1104 76-77 -0.2223762332542627 1104 78-79 -0.13993115286230662 1104 80-81 -0.1688318692329105 1104 82-83 -0.2992122340210166 1104 84-85 -0.17310791043064455 1104 86-87 -0.03498544685194105 1104 88-89 0.1588244590966461 1104 90-91 0.0407713373233598 1104 92-93 0.15889353790648997 1104 94-95 0.10815874075305842 1104 96-97 0.07016758612176943 1104 98-99 0.32059227608754526 1104 100-101 0.8404863131461298 1105 1 -0.031446470715692953 1105 2 -0.03832645308327187 1105 3 -0.007221538575898023 1105 4 0.023157270967111288 1105 5 -0.006639843808621038 1105 6 -0.04264744005175203 1105 7 -0.0925445528202502 1105 8 -0.02492428906217725 1105 9 0.04739747875640887 1105 10-11 -0.023320037492837287 1105 12-13 -0.022073415382642736 1105 14-15 0.022997346927013496 1105 16-17 -0.02538508570468423 1105 18-19 -0.0944224954762376 1105 20-21 -0.05461257140188991 1105 22-23 -0.07878327312017319 1105 24-25 -0.05767028729329127 1105 26-27 -0.03988930020496895 1105 28-29 -0.0013702088119060818 1105 30-31 -0.13985710606880986 1105 32-33 -0.06554801745424044 1105 34-35 0.003860693031441542 1105 36-37 -0.08406484573039563 1105 38-39 -0.11804857382348644 1105 40-41 -0.08851238228277936 1105 42-43 -0.10023839698464343 1105 44-45 -0.12086761789392142 1105 46-47 -0.017540003342524813 1105 48-49 -0.0480178632539392 1105 50-51 0.02079798435637059 1105 52-53 0.009008101991270223 1105 54-55 -0.049786500490803576 1105 56-57 -0.07716642398163742 1105 58-59 -0.09742741268347288 1105 60-61 -0.12180127260373297 1105 62-63 -0.22988261492282902 1105 64-65 -0.12445685961458963 1105 66-67 -0.0727409816216138 1105 68-69 -0.01323093514098872 1105 70-71 -0.09559521197649445 1105 72-73 -0.26607665874470143 1105 74-75 -0.1704279944215088 1105 76-77 -0.18091832919165896 1105 78-79 -0.2675789282611518 1105 80-81 -0.09035572717701967 1105 82-83 -0.20334475478821545 1105 84-85 -0.12321784288832305 1105 86-87 -0.05451502765998484 1105 88-89 0.1488193749651785 1105 90-91 0.12926738523612968 1105 92-93 0.04725798008386661 1105 94-95 0.09535472758913244 1105 96-97 -0.05635499659464216 1105 98-99 -0.0011929755685748944 1105 100-101 0.4236248666352225 1106 1 -0.09282224946710116 1106 2 -0.06933061644923555 1106 3 -0.06308525145733768 1106 4 -0.024035843930917622 1106 5 -0.04606337944970562 1106 6 -0.05058726232025634 1106 7 -0.09377805587234889 1106 8 -0.09051220274648841 1106 9 -0.04248883091866418 1106 10-11 -0.09716318518403 1106 12-13 -0.03856546569620889 1106 14-15 0.08715826823694073 1106 16-17 -0.1431853514714163 1106 18-19 -0.08800102979152058 1106 20-21 -0.04454567721109726 1106 22-23 -0.08021481818449416 1106 24-25 -0.11345294849783016 1106 26-27 -0.1535327876096062 1106 28-29 -0.07898746748001884 1106 30-31 0.0394136826860958 1106 32-33 -0.05606724760264825 1106 34-35 -0.015859352407161964 1106 36-37 0.03653718681113105 1106 38-39 0.06906201598162909 1106 40-41 0.05352357651785411 1106 42-43 0.07061570972103226 1106 44-45 0.1185103011736004 1106 46-47 0.14951584933353246 1106 48-49 0.10302035009393506 1106 50-51 0.14696271419835938 1106 52-53 0.15881108778329178 1106 54-55 0.13897913006641005 1106 56-57 -0.025776556724274258 1106 58-59 -0.0015610413070348272 1106 60-61 0.02212881215079676 1106 62-63 -0.030926167334357046 1106 64-65 -0.015409312700491284 1106 66-67 -0.04480753863742848 1106 68-69 -0.033059313906029786 1106 70-71 -0.10935037134685643 1106 72-73 0.032190959958413146 1106 74-75 0.1340163483921728 1106 76-77 0.12308709292409858 1106 78-79 0.11596419440404304 1106 80-81 0.023824839679782883 1106 82-83 0.14895632646184964 1106 84-85 -0.017289095520951037 1106 86-87 0.0593001858294393 1106 88-89 0.2471334380036545 1106 90-91 0.3553589340364134 1106 92-93 0.415676376979647 1106 94-95 0.36367340677928084 1106 96-97 0.4234363370703491 1106 98-99 0.5098636113322073 1106 100-101 1.1261051307243477 1107 1 -0.12948878002934805 1107 2 -0.1005446414611697 1107 3 -0.2018605676411056 1107 4 -0.07811622395070117 1107 5 -0.04785426983871588 1107 6 -0.11884261289984721 1107 7 -0.05287177107811658 1107 8 0.0037902834343412906 1107 9 -0.11365966004444061 1107 10-11 -0.05363221807681384 1107 12-13 -0.09228628627991498 1107 14-15 -0.14858078180702705 1107 16-17 -0.11447138181538463 1107 18-19 -0.050515028740470314 1107 20-21 -0.024141491776745738 1107 22-23 -0.16858619879725012 1107 24-25 -0.16327864342770226 1107 26-27 -0.10312580492472989 1107 28-29 -0.07482724291990905 1107 30-31 -0.1986084682065794 1107 32-33 0.04003547344971281 1107 34-35 -0.07264587425059688 1107 36-37 -0.10058567952356157 1107 38-39 -0.05653757984919139 1107 40-41 -0.110196327900276 1107 42-43 -0.09360006169342938 1107 44-45 -0.14705257896655155 1107 46-47 -0.24989597643411798 1107 48-49 -0.3132064656862923 1107 50-51 -0.3489084465143435 1107 52-53 -0.3371041545100013 1107 54-55 -0.36824354549864324 1107 56-57 -0.4292052911299109 1107 58-59 -0.36977570275948324 1107 60-61 -0.386285609961007 1107 62-63 -0.36497394729860844 1107 64-65 -0.2862086580179195 1107 66-67 -0.44141094112602985 1107 68-69 -0.39998108494548745 1107 70-71 -0.5143638081032904 1107 72-73 -0.42407503358048615 1107 74-75 -0.4080936479492543 1107 76-77 -0.43729878138733014 1107 78-79 -0.37480355843683455 1107 80-81 -0.4683428588412042 1107 82-83 -0.4909501799591318 1107 84-85 -0.4559555687974566 1107 86-87 -0.42428958244973813 1107 88-89 -0.32360886534221933 1107 90-91 -0.11344410478930556 1107 92-93 -0.087519232901208 1107 94-95 -0.053301780166233215 1107 96-97 -0.014372779934966218 1107 98-99 -0.11927144914589505 1107 100-101 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#Quality Count 2 3.0 3 18.0 4 35.0 5 90.0 6 216.0 7 569.0 8 1226.0 9 2381.0 10 3768.0 11 5932.0 12 8276.0 13 11105.0 14 13675.0 15 17926.0 16 22453.0 17 29775.0 18 28399.0 19 21039.0 20 19852.0 21 20895.0 22 22488.0 23 25619.0 24 30092.0 25 35946.0 26 43962.0 27 54039.0 28 66479.0 29 80980.0 30 98910.0 31 120356.0 32 147299.0 33 186545.0 34 245261.0 35 341269.0 36 512043.0 37 817653.0 38 749913.0 39 85246.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30183177800772 20.009655265036248 14.933563013179022 22.75494994377701 2 15.35885674141342 21.280819476514434 39.87393265043624 23.486391131635905 3 19.739651939438083 25.13433236735938 29.93173611864433 25.19427957455821 4 13.007200896754432 17.22925609674203 37.64031159117089 32.12323141533264 5 13.07306275731325 38.28604193463404 34.60862557919695 14.03226972885576 6 30.949082324742882 37.11181535950245 17.504816955684007 14.434285360070668 7 27.806490105224007 33.05978707247904 21.084086224798153 18.0496365974988 8 25.480300846647765 35.71223790853725 19.713539648840054 19.093921595974937 9 25.76763935594849 17.461658065882524 20.869299074834572 35.901403503334414 10-11 24.609025915996423 27.0264661366724 27.50144637811423 20.863061569216942 12-13 25.808267084050062 24.310116381781835 27.865313786566247 22.016302747601856 14-15 22.490924923622654 25.868381076627106 26.755193833868347 24.885500165881897 16-17 21.44369694526921 29.063328417686463 27.354044483095773 22.138930153948554 18-19 21.316699272638658 28.182046057135373 29.340325376133098 21.160929294092867 20-21 22.503528452779427 26.882025508172745 29.228528933829356 21.385917105218468 22-23 21.871393740282148 26.910148925289405 29.129383687954252 22.089073646474194 24-25 21.969954077494876 26.997719371652025 29.336280328741083 21.696046222112024 26-27 21.842597698447854 26.863589579348794 29.664349445403676 21.629463276799676 28-29 21.42567682747292 27.53138123944273 29.469592482966316 21.573349450118034 30-31 21.79584631199409 27.130321250910445 29.244022067597513 21.829810369497952 32-33 21.632483069627607 27.221365587565284 29.243557160346512 21.9025941824606 34-35 22.13832530332882 27.124289746585067 29.213563249119996 21.52382170096612 36-37 22.095874790882423 26.852053253037695 29.480838164952566 21.571233791127316 38-39 21.74913558650638 27.30720036969813 29.37346214733602 21.570201896459466 40-41 21.98942013238331 27.285914858658067 29.158282015891114 21.566382993067513 42-43 21.592498904521122 27.383045925909855 28.841088600575294 22.18336656899373 44-45 21.858009099222436 27.23811904977452 28.816554804354062 22.08731704664898 46-47 22.152247993659575 27.52500314141454 28.394526022212418 21.928222842713474 48-49 21.9912822063175 27.468424052101685 28.535516298694397 22.004777442886418 50-51 21.773149688878625 27.728150831135668 28.596253267765583 21.902446212220124 52-53 22.052345973644925 27.854930857960504 28.137181215680805 21.955541952713766 54-55 21.779781183653864 27.597811319974998 28.35127100476788 22.271136491603258 56-57 22.011666589182123 27.696836564438726 28.09756078662307 22.193936059756076 58-59 22.244179167467884 27.420975982628903 28.18311816832758 22.15172668157564 60-61 21.96849898488177 27.614353995185343 28.5723657710992 21.84478124883369 62-63 21.846262920547353 27.521088709469126 28.582032884439556 22.050615485543968 64-65 21.63426521408979 27.868051573711046 28.343212612417158 22.154470599782012 66-67 21.774705328558497 27.604931702863222 28.04065929950396 22.57970366907432 68-69 21.716458696222645 27.603737269171404 28.200425445746884 22.479378588859067 70-71 22.00209367990718 27.365527686889212 28.01049784433105 22.621880788872552 72-73 21.907088285886967 27.19096581898578 28.27408348701101 22.627862408116247 74-75 21.507565074101052 28.00406277281016 27.889437307257204 22.59893484583159 76-77 21.733342209473903 27.309540536924636 28.14616418231679 22.810953071284672 78-79 21.799780227559815 27.377830061076896 28.127203440914002 22.695186270449287 80-81 21.95279975649182 27.443873081820996 27.848884938897605 22.75444222278958 82-83 21.847851353654946 27.329249897978684 28.14384488627852 22.679053862087848 84-85 21.744461146667906 27.349034284326944 27.95018518805498 22.956319380950166 86-87 21.61969694186984 27.439813505872824 27.982112125662113 22.958377426595227 88-89 21.748363474664323 27.298381490922097 27.774837332173796 23.17841770223978 90-91 21.744327770789216 27.05586214426058 27.8753176097383 23.3244924752119 92-93 21.86696420730731 27.266435762732 27.88217958850543 22.984420441455264 94-95 21.67194121297949 27.111189571404477 27.865630921919067 23.351238293696966 96-97 21.342665054988196 26.98657451171825 28.16213123815132 23.508629195142237 98-99 21.56076781200337 27.136519627277632 27.65844406981997 23.64426849089903 100-101 21.587948812387676 26.625583268202902 28.091469988258446 23.694997931150976 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2256.0 1 1450.5 2 828.0 3 1065.5 4 920.5 5 894.0 6 1747.0 7 3626.5 8 5154.0 9 5389.0 10 4599.5 11 3727.0 12 3569.5 13 3700.5 14 3944.5 15 4173.0 16 4424.5 17 4722.5 18 5077.0 19 5645.0 20 6498.0 21 7461.0 22 8931.5 23 11215.5 24 13824.0 25 17142.5 26 22493.5 27 29114.0 28 34349.0 29 40439.5 30 47461.0 31 54806.5 32 63596.0 33 71801.5 34 80468.0 35 89679.5 36 100056.5 37 108763.0 38 112903.5 39 116053.5 40 119764.0 41 123582.0 42 125641.0 43 129762.5 44 135653.5 45 139504.5 46 141398.0 47 152098.0 48 179130.0 49 176281.0 50 150000.5 51 138036.0 52 129494.0 53 123083.5 54 117149.5 55 108621.0 56 98534.0 57 87854.0 58 78351.0 59 69525.5 60 57993.5 61 46493.0 62 36820.5 63 28059.5 64 20461.0 65 15067.0 66 11002.5 67 8085.5 68 6023.0 69 4331.5 70 3131.0 71 2120.0 72 1545.0 73 1320.5 74 1029.0 75 698.0 76 479.0 77 290.5 78 181.0 79 115.5 80 69.5 81 41.0 82 29.0 83 21.0 84 11.5 85 6.5 86 4.5 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3816630248932005 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.453677158073631E-4 16-17 0.0023116221647114733 18-19 1.4205499336215758E-4 20-21 1.2914090305650689E-5 22-23 0.0 24-25 0.0 26-27 1.291409030565069E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008329588247144694 36-37 0.01838966459524658 38-39 0.012229643519451203 40-41 0.008536213692035105 42-43 0.0037580002789443503 44-45 0.0023374503453227747 46-47 0.001975855816764555 48-49 9.039863213955482E-5 50-51 5.036495219203768E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.553058831429796E-4 60-61 6.069622443655824E-4 62-63 0.0 64-65 0.0 66-67 0.0014205499336215758 68-69 9.298145020068496E-4 70-71 0.0022728798937945213 72-73 0.0 74-75 0.0 76-77 0.09537055690723034 78-79 0.035462091979316795 80-81 3.874227091695207E-5 82-83 0.0 84-85 0.0 86-87 0.1518955301750634 88-89 0.32867651236911566 90-91 0.2858275607349667 92-93 0.0 94-95 2.5828180611301377E-5 96-97 0.0 98-99 2.8410998672431516E-4 100-101 5.682199734486303E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3871740.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.4830448389325 #Duplication Level Percentage of deduplicated Percentage of total 1 68.25631323613072 32.410175819314176 2 16.174327465797493 15.360126325960795 3 6.2802355451344045 8.946141179660238 4 3.1924268573828325 6.063445904564856 5 1.7874813579601454 4.2437528734388765 6 1.0597283867977307 3.019147830444375 7 0.7096872480771308 2.358867799544551 8 0.4928762843738112 1.8722613368774499 9 0.34739019397493537 1.4845629741405582 >10 1.5465525084126257 12.691278770079318 >50 0.09777353611134351 3.219546867240669 >100 0.05079658427494342 4.378002530757321 >500 0.002395987750841187 0.7790059537008821 >1k 0.001742536493829602 1.8011150912319889 >5k 1.0890853086434995E-4 0.3759024260864601 >10k+ 1.6336279629652493E-4 0.9966663169575383 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14134 0.36505550476013365 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13316 0.34392805302008916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11096 0.2865894920630001 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7657 0.19776637894073465 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6881 0.17772371078636479 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4771 0.12322624969651888 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4748 0.12263220154245895 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3930 0.1015047498024144 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0662544489041102E-4 2 2.5828180611301377E-5 0.0 0.0 2.5828180611301377E-5 2.0662544489041102E-4 3 2.5828180611301377E-5 0.0 0.0 2.5828180611301377E-5 2.0662544489041102E-4 4 2.5828180611301377E-5 0.0 0.0 5.1656361222602755E-5 2.0662544489041102E-4 5 2.5828180611301377E-5 0.0 0.0 5.1656361222602755E-5 2.3245362550171242E-4 6 2.5828180611301377E-5 0.0 0.0 5.1656361222602755E-5 2.8410998672431516E-4 7 2.5828180611301377E-5 0.0 0.0 5.1656361222602755E-5 2.8410998672431516E-4 8 2.5828180611301377E-5 0.0 0.0 5.1656361222602755E-5 2.8410998672431516E-4 9 2.5828180611301377E-5 0.0 0.0 7.748454183390414E-5 2.8410998672431516E-4 10-11 2.5828180611301377E-5 0.0 0.0 1.0331272244520551E-4 3.2285225764126725E-4 12-13 7.748454183390414E-5 0.0 0.0 1.1622681275085621E-4 8.910722310898976E-4 14-15 7.748454183390414E-5 0.0 0.0 1.291409030565069E-4 0.0010976976759803085 16-17 9.039863213955482E-5 0.0 0.0 1.291409030565069E-4 0.0012655808499537676 18-19 1.2914090305650688E-4 0.0 0.0 1.291409030565069E-4 0.0012655808499537676 20-21 1.5496908366780828E-4 0.0 0.0 1.9371135458476035E-4 0.0015626049269837333 22-23 1.5496908366780828E-4 0.0 0.0 4.1325088978082204E-4 0.001704659920345891 24-25 1.5496908366780828E-4 0.0 0.0 8.652440504785962E-4 0.0017304881009571923 26-27 1.5496908366780828E-4 0.0 0.0 0.0018208867330967472 0.0018983712749306514 28-29 2.0662544489041102E-4 0.0 0.0 0.004261649800864728 0.0019371135458476033 30-31 2.3245362550171242E-4 1.2914090305650689E-5 0.0 0.012617066228620723 0.0019887699070702063 32-33 2.3245362550171242E-4 2.5828180611301377E-5 0.0 0.028333514130597613 0.00205334035859846 34-35 2.453677158073631E-4 2.5828180611301377E-5 0.0 0.055595158765826214 0.0021437389907380143 36-37 2.582818061130138E-4 2.5828180611301377E-5 0.0 0.09937392490198205 0.0021437389907380143 38-39 2.582818061130138E-4 2.5828180611301377E-5 0.0 0.164887105022548 0.002156653081043665 40-41 2.9702407702996586E-4 2.5828180611301377E-5 0.0 0.23524306900773295 0.0021695671713493157 42-43 3.357663479469179E-4 2.5828180611301377E-5 0.0 0.30700666883623384 0.0022212235325719185 44-45 4.0033679947517134E-4 2.5828180611301377E-5 0.0 0.4006983940037296 0.0022728798937945213 46-47 4.907354316147262E-4 2.5828180611301377E-5 0.0 0.5136062855460335 0.0023374503453227747 48-49 4.907354316147262E-4 2.5828180611301377E-5 0.0 0.6324159163580199 0.002453677158073631 50-51 4.907354316147262E-4 2.5828180611301377E-5 0.0 0.7574113964264129 0.0025569898805188366 52-53 4.907354316147262E-4 2.5828180611301377E-5 0.0 0.8842019350472914 0.002686130783575343 54-55 4.907354316147262E-4 2.5828180611301377E-5 0.0 1.0274837669884858 0.002776529415714898 56-57 5.682199734486303E-4 3.874227091695207E-5 0.0 1.2049104536978206 0.00281527168663185 58-59 5.682199734486303E-4 7.748454183390414E-5 0.0 1.3977953065030193 0.0028410998672431516 60-61 5.682199734486303E-4 1.291409030565069E-4 0.0 1.6063062085780553 0.0028410998672431516 62-63 5.682199734486303E-4 1.291409030565069E-4 0.0 1.8173353582626932 0.00299606895091096 64-65 5.682199734486303E-4 1.291409030565069E-4 0.0 2.0350152644547412 0.0030606394024392134 66-67 5.940481540599317E-4 1.291409030565069E-4 0.0 2.2860006095450625 0.003073553492744864 68-69 5.940481540599317E-4 1.291409030565069E-4 0.0 2.569606946747457 0.003073553492744864 70-71 6.198763346712331E-4 1.291409030565069E-4 0.0 2.8830835748268218 0.0031122957636618162 72-73 6.586186055881852E-4 1.291409030565069E-4 0.0 3.1958111856684592 0.0032414366667183232 74-75 6.973608765051372E-4 1.291409030565069E-4 0.0 3.520626385036185 0.0033834916600804805 76-77 6.973608765051372E-4 1.291409030565069E-4 0.0 3.8660912148026467 0.003409319840691782 78-79 6.973608765051372E-4 1.291409030565069E-4 0.0 4.268700894171613 0.0035513748340539396 80-81 8.265017795616441E-4 1.291409030565069E-4 0.0 4.699786142664538 0.0036288593758878438 82-83 8.265017795616441E-4 1.291409030565069E-4 0.0 5.137134724955705 0.0036934298274160973 84-85 8.652440504785962E-4 1.291409030565069E-4 0.0 5.584181789066415 0.0036934298274160973 86-87 9.039863213955483E-4 1.291409030565069E-4 0.0 6.050328792739181 0.0037580002789443507 88-89 9.039863213955483E-4 1.291409030565069E-4 0.0 6.551615036133624 0.003835484820778255 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 17415 0.0 21.251184 1 TACATGG 17380 0.0 21.050688 2 GAGTACT 13240 0.0 20.591814 12-13 AGTACTT 14110 0.0 19.675303 12-13 ACATGGG 17710 0.0 19.640255 3 GTACTTT 14030 0.0 19.31407 14-15 TACCTGG 2960 0.0 18.275736 2 CATGGGG 7525 0.0 17.71991 4 TAGCCCT 3020 0.0 17.441256 4 GGACCGA 720 0.0 17.135729 6 CGCTATA 170 0.0017560564 16.810783 1 CCGATCG 285 8.683928E-7 16.650316 9 GTATCAA 35845 0.0 16.636477 1 CCTATAC 1035 0.0 16.505316 3 AGAGTAC 27310 0.0 16.455656 10-11 TACTTTT 16745 0.0 16.38091 14-15 CATGGGA 10145 0.0 16.183996 4 GTATAGG 1270 0.0 16.12688 1 ACTTTTT 17035 0.0 16.031883 16-17 GTATACG 240 6.010633E-5 15.87685 1 >>END_MODULE