FastQCFastQC Report
Thu 26 May 2016
SRR938790_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938790_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885380
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135620.34905208756929823No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT130010.3346133454128039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104790.2697033494793302No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74340.19133263670477535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69460.17877273265420626No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50770.1306693296408588No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50380.1296655668171453No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG173250.024.752132
GTACATG176700.024.7177931
ACATGGG178800.022.6841033
GAGTACT124000.021.24354212-13
CATGGGG77850.019.5600244
TACCTGG30050.019.2589552
GTACTTT138200.018.99245514-15
AGTACTT134400.018.82277112-13
GTGTAGC26500.018.6516881
CATGGGA104100.018.3644244
GTATCAA347150.017.3456341
AGTACAT132950.017.3049682
GTACCTG37300.016.309171
AGAGTAC256400.016.28849810-11
GTATAGC22550.016.2283691
ACTTTTT164700.016.1533616-17
CAACGCA382050.015.6076695
TATCAAC384400.015.5490752
TCAACGC384200.015.5450234
ATCAACG384750.015.5104713
Produced by FastQC (version 0.11.5)