FastQCFastQC Report
Thu 26 May 2016
SRR938790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885380
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT142330.3663219556388307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132660.3414337851123957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112560.2897013934286994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77750.20010912703519348No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69710.17941617036171495No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49170.12655132831280339No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48230.12413200253257081No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39080.10058218243775383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG12500.022.0738161
GTACATG177700.021.7919671
TACATGG177800.021.5682412
ACATGGG181200.020.1158393
GAGTACT129900.019.88895812-13
TACCTGG29800.018.6339422
GTACTTT139600.018.3540414-15
AGTACTT140600.018.12225712-13
CATGGGG73750.017.182424
CATGGGA104950.016.7322524
ACTTTTT162600.015.94689616-17
GTATAGA13600.015.7410261
GTACCTG37850.015.7109911
AGTACAT140450.015.6456782
AGAGTAC270650.015.63796210-11
GTGTAGG15400.015.445741
GTATCAA359700.015.4211781
TACTTTT163550.015.39069314-15
CCGATCG3555.2832183E-714.7012459
GTGTAGC25650.014.6520761
Produced by FastQC (version 0.11.5)