##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938790_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3885380 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05675892705475 33.0 31.0 34.0 30.0 34.0 2 32.25696070911983 34.0 31.0 34.0 30.0 34.0 3 32.34453515486259 34.0 31.0 34.0 30.0 34.0 4 35.95839763420824 37.0 35.0 37.0 35.0 37.0 5 35.86291147841395 37.0 35.0 37.0 35.0 37.0 6 35.89029670199569 37.0 35.0 37.0 35.0 37.0 7 35.81012719476602 37.0 35.0 37.0 33.0 37.0 8 35.796844581482375 37.0 35.0 37.0 33.0 37.0 9 37.44447724546891 39.0 37.0 39.0 34.0 39.0 10-11 37.368054990760236 39.0 37.0 39.0 34.0 39.0 12-13 37.293296665963176 39.0 37.0 39.0 34.0 39.0 14-15 38.50095280255728 40.0 38.0 41.0 34.0 41.0 16-17 38.43328220148351 40.0 38.0 41.0 33.0 41.0 18-19 38.587516665036624 40.0 38.0 41.0 34.0 41.0 20-21 38.58886646865943 40.0 38.0 41.0 34.0 41.0 22-23 38.514036593589296 40.0 38.0 41.0 34.0 41.0 24-25 38.445530552996104 40.0 38.0 41.0 34.0 41.0 26-27 38.27014835099784 40.0 38.0 41.0 33.5 41.0 28-29 38.110653012060595 40.0 37.5 41.0 33.0 41.0 30-31 37.9091386170722 40.0 37.0 41.0 33.0 41.0 32-33 37.76932809660831 40.0 37.0 41.0 33.0 41.0 34-35 37.752131580437435 40.0 37.0 41.0 33.0 41.0 36-37 37.6510865089129 40.0 37.0 41.0 32.5 41.0 38-39 37.535423948236726 40.0 37.0 41.0 32.0 41.0 40-41 37.38263065646089 40.0 37.0 41.0 31.5 41.0 42-43 37.202321909311316 40.0 36.0 41.0 31.0 41.0 44-45 36.975229192511414 40.0 36.0 41.0 31.0 41.0 46-47 36.70184229084414 39.5 36.0 41.0 30.0 41.0 48-49 36.42481893662911 39.0 35.5 41.0 30.0 41.0 50-51 36.20546278613675 39.0 35.0 41.0 29.5 41.0 52-53 36.00029559528283 39.0 35.0 40.5 29.0 41.0 54-55 35.74652891094307 39.0 35.0 40.0 28.0 41.0 56-57 35.48612349371233 38.0 35.0 40.0 28.0 41.0 58-59 35.196230355846794 38.0 34.0 40.0 27.5 41.0 60-61 34.83833537517566 38.0 34.0 40.0 26.5 41.0 62-63 34.486018870741084 37.0 34.0 40.0 26.0 41.0 64-65 34.098571954351954 37.0 33.0 39.5 26.0 41.0 66-67 33.69473809511554 36.0 33.0 39.0 25.5 40.5 68-69 33.34483692714741 36.0 32.5 39.0 24.5 40.0 70-71 32.916160324086704 35.0 32.0 38.5 23.5 40.0 72-73 32.423138406024634 35.0 31.5 37.5 22.5 40.0 74-75 31.92273226814366 35.0 31.0 37.0 21.5 39.0 76-77 29.962827188074268 33.0 28.5 35.0 19.5 37.5 78-79 30.926311583422986 34.0 30.5 36.0 19.5 38.0 80-81 30.97557845049905 34.0 31.0 36.0 20.0 37.5 82-83 30.641955484405642 34.0 31.0 35.0 18.0 37.0 84-85 30.226548111124266 34.0 30.0 35.0 15.0 36.5 86-87 29.647999166104732 34.0 29.5 35.0 7.5 36.0 88-89 29.027597043275048 34.0 29.0 35.0 2.0 36.0 90-91 28.492597249175112 33.5 29.0 35.0 2.0 35.0 92-93 28.186707220400578 33.0 28.5 35.0 2.0 35.0 94-95 27.832582656007908 33.0 27.5 35.0 2.0 35.0 96-97 27.205137721406913 33.0 26.5 35.0 2.0 35.0 98-99 26.360255110182273 33.0 25.0 35.0 2.0 35.0 100-101 22.6337919328354 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10070729948200352 1101 2 0.09317319395265855 1101 3 0.08892393245604069 1101 4 0.11713201765408598 1101 5 -0.048806058423551235 1101 6 0.04850972286729416 1101 7 0.1605715844294835 1101 8 0.16016836913187404 1101 9 0.23649069862518957 1101 10-11 0.23894450310662307 1101 12-13 0.18921318926918218 1101 14-15 0.3419520697462133 1101 16-17 0.32896459613962037 1101 18-19 0.31663432736884545 1101 20-21 0.2964772108291527 1101 22-23 0.1731251133557521 1101 24-25 0.20203551588612356 1101 26-27 0.12624985173374625 1101 28-29 0.23169327051054722 1101 30-31 0.1828483918542858 1101 32-33 0.286038987138852 1101 34-35 0.32404388820990704 1101 36-37 0.2722352769077432 1101 38-39 0.36581765481241035 1101 40-41 0.4235031274740919 1101 42-43 0.44154294920070924 1101 44-45 0.5096394821698951 1101 46-47 0.41235804892377814 1101 48-49 0.34527534076269006 1101 50-51 0.31836164583260285 1101 52-53 0.22880390722102817 1101 54-55 0.3325895815904971 1101 56-57 0.27665440373416317 1101 58-59 0.3001056246389311 1101 60-61 0.3944511927603642 1101 62-63 0.18122228163013432 1101 64-65 0.12302110820646561 1101 66-67 0.139859856722083 1101 68-69 0.14635604342180386 1101 70-71 0.2995435620239988 1101 72-73 0.1706592980277506 1101 74-75 0.1432063379352435 1101 76-77 0.13268675943168518 1101 78-79 0.06573542290011147 1101 80-81 0.18218067037571473 1101 82-83 0.17614006766072166 1101 84-85 0.33823472890817996 1101 86-87 0.3853608635482253 1101 88-89 0.4702182328573308 1101 90-91 0.3611319446663792 1101 92-93 0.4035577661429919 1101 94-95 0.6638281972485025 1101 96-97 0.638564299012085 1101 98-99 0.3872172078346452 1101 100-101 0.9487907396754913 1102 1 0.19321872960811248 1102 2 0.1517818635705055 1102 3 0.1477845417753656 1102 4 0.08833220720289603 1102 5 -0.07552829982469689 1102 6 -0.024256450816309894 1102 7 0.03687251761115107 1102 8 0.07243306671767158 1102 9 0.14496340159330146 1102 10-11 0.1569494000106033 1102 12-13 0.177369341645516 1102 14-15 0.20604363567264272 1102 16-17 0.2609103601954743 1102 18-19 0.25919933965862185 1102 20-21 0.25366657260567393 1102 22-23 0.18258605676165018 1102 24-25 0.22919310175930718 1102 26-27 0.09673869011249536 1102 28-29 0.18200079512999423 1102 30-31 0.1586354318615122 1102 32-33 0.18956692369430783 1102 34-35 0.2604271787216348 1102 36-37 0.2650195907929742 1102 38-39 0.27403702633453264 1102 40-41 0.2843216837054925 1102 42-43 0.2886641256796807 1102 44-45 0.2409687712973465 1102 46-47 0.24881367620509565 1102 48-49 0.21487845256763194 1102 50-51 0.2213999938250808 1102 52-53 0.030114115870681246 1102 54-55 0.07560898149063178 1102 56-57 0.052663477908375 1102 58-59 0.04132532916139553 1102 60-61 0.0360550527128467 1102 62-63 0.027695855626639343 1102 64-65 0.10594212874217135 1102 66-67 0.14515215192263753 1102 68-69 0.1528263654884583 1102 70-71 0.09860875099744959 1102 72-73 0.11414323508966362 1102 74-75 0.03689382611814196 1102 76-77 0.050464089135619616 1102 78-79 -0.11652434926791955 1102 80-81 0.24493986157527559 1102 82-83 0.16131456017466306 1102 84-85 0.29154994919587907 1102 86-87 0.35445979044531484 1102 88-89 0.2521366881702818 1102 90-91 0.29856108026065087 1102 92-93 0.33755530105796083 1102 94-95 0.435973347375306 1102 96-97 0.4311171420236448 1102 98-99 0.6218046726973405 1102 100-101 1.23467729620425 1103 1 0.21281699335353466 1103 2 0.170827477933841 1103 3 0.09163519131163866 1103 4 0.032087572362947014 1103 5 0.03795712469752033 1103 6 0.05885404700990193 1103 7 0.08895409143185873 1103 8 0.04670368647324352 1103 9 0.16119033086371104 1103 10-11 0.10824695665932893 1103 12-13 0.07489402679561863 1103 14-15 0.17485965800036496 1103 16-17 0.10829294169177217 1103 18-19 0.03391675651651127 1103 20-21 0.03974621821708979 1103 22-23 0.1774151469084586 1103 24-25 0.2143932725782065 1103 26-27 0.2166410842623847 1103 28-29 0.174478526738163 1103 30-31 0.2744133118534364 1103 32-33 0.40735495753585127 1103 34-35 0.2249346148009792 1103 36-37 0.29586530892966323 1103 38-39 0.2062876692875193 1103 40-41 0.35170679456886234 1103 42-43 0.28019637640925765 1103 44-45 0.2594392145169593 1103 46-47 0.35573660587049716 1103 48-49 0.4246240519453366 1103 50-51 0.37780973050237776 1103 52-53 0.22200212381221007 1103 54-55 0.19583187763326038 1103 56-57 0.1327651550000155 1103 58-59 0.1370657710556813 1103 60-61 0.08062215413612961 1103 62-63 0.1220668011681596 1103 64-65 0.14930258347386172 1103 66-67 0.25077704851742766 1103 68-69 0.251699930917205 1103 70-71 0.24059416555811453 1103 72-73 0.29855488438425937 1103 74-75 0.35186715948612957 1103 76-77 0.35888532230601555 1103 78-79 0.11774961410303675 1103 80-81 0.30038713943057616 1103 82-83 0.3444862132459825 1103 84-85 0.37904526077145917 1103 86-87 0.4020539032568209 1103 88-89 0.18668714947659382 1103 90-91 0.20136192118858176 1103 92-93 0.32268530792709527 1103 94-95 0.4921867982375865 1103 96-97 0.5312016852519967 1103 98-99 0.7406944537944788 1103 100-101 1.3678865866041754 1104 1 0.1368093511648354 1104 2 0.08400500765805674 1104 3 0.0589759868651214 1104 4 0.03952382788604325 1104 5 -0.0972294486444909 1104 6 0.023990029633559118 1104 7 0.032601208255094605 1104 8 0.01922168299781646 1104 9 0.04475444545214202 1104 10-11 0.07293344735058582 1104 12-13 0.032717696993870504 1104 14-15 0.0292627894002635 1104 16-17 0.12460708406169374 1104 18-19 0.0725589242046567 1104 20-21 0.1355185561525758 1104 22-23 0.10885548726837158 1104 24-25 0.08900811411669451 1104 26-27 0.04226632631809224 1104 28-29 0.12512938656367822 1104 30-31 -0.018595372487943962 1104 32-33 0.053765186197658466 1104 34-35 0.05068964695020384 1104 36-37 0.1702002544482184 1104 38-39 0.15533589068424902 1104 40-41 0.12896981648921013 1104 42-43 0.15139508586827333 1104 44-45 0.07176587751818886 1104 46-47 0.09313147391272025 1104 48-49 0.18088905840874503 1104 50-51 0.019643181898779005 1104 52-53 0.05555430656511362 1104 54-55 -0.034055679021484764 1104 56-57 -0.19475639663014732 1104 58-59 -0.06565165493423564 1104 60-61 0.025552372718635752 1104 62-63 -0.028637798164638184 1104 64-65 -0.09332015950317185 1104 66-67 -0.11495105309582954 1104 68-69 0.01191047033527326 1104 70-71 0.05308616310197323 1104 72-73 0.1227429216554512 1104 74-75 0.12369842139731091 1104 76-77 0.050888805662033576 1104 78-79 -0.1223364906732769 1104 80-81 -0.0012300074759501456 1104 82-83 0.0930548224234009 1104 84-85 0.16439289407272284 1104 86-87 0.3865253394600181 1104 88-89 0.3720571004635751 1104 90-91 0.2952094413157944 1104 92-93 0.27164631989473875 1104 94-95 0.39064281475663876 1104 96-97 0.40166793460833716 1104 98-99 0.6122743411331086 1104 100-101 1.1417818050036708 1105 1 0.13827946117717715 1105 2 0.10404931988040289 1105 3 0.09436308815179473 1105 4 0.09337145706276573 1105 5 -0.05200796149890152 1105 6 -0.008495781190056562 1105 7 0.01838789553885789 1105 8 0.09066313825749717 1105 9 0.09205386283232286 1105 10-11 0.09116519918345034 1105 12-13 0.08898272218259251 1105 14-15 0.13651963600715078 1105 16-17 0.1577384118997287 1105 18-19 0.1549574938806515 1105 20-21 0.10246048077300429 1105 22-23 0.07195194979100705 1105 24-25 0.053408781836537855 1105 26-27 0.0958337924162862 1105 28-29 0.07064707576717666 1105 30-31 0.08051007809189059 1105 32-33 0.09823857693393023 1105 34-35 0.15884051093991758 1105 36-37 0.1224508762181955 1105 38-39 0.1875068262368771 1105 40-41 0.12011496328350546 1105 42-43 0.18973914689113514 1105 44-45 0.1901474303042079 1105 46-47 0.027558909599136427 1105 48-49 0.08533333502352747 1105 50-51 0.10062289881857822 1105 52-53 0.07288302233337873 1105 54-55 0.16632807562053387 1105 56-57 0.23362080123558115 1105 58-59 0.17846122210829662 1105 60-61 0.146226617154241 1105 62-63 0.15477078601132632 1105 64-65 0.032833939792645594 1105 66-67 0.11146386128638852 1105 68-69 0.13547776514339205 1105 70-71 0.13518062801904307 1105 72-73 0.15546542421377296 1105 74-75 0.1761029317244187 1105 76-77 0.20622781336826534 1105 78-79 -0.087785855900826 1105 80-81 -0.0033665267007947364 1105 82-83 0.13244758095396847 1105 84-85 0.1761181536225962 1105 86-87 0.18117235604115578 1105 88-89 0.17786132940780774 1105 90-91 0.2324887269220035 1105 92-93 0.1585889686050166 1105 94-95 0.3179909096606792 1105 96-97 0.48665486595449536 1105 98-99 0.6582458062834959 1105 100-101 1.4214275745411875 1106 1 0.09050493258738612 1106 2 0.047692223837302095 1106 3 0.03683440830047857 1106 4 -0.03299774630526997 1106 5 -0.037598994073604786 1106 6 -0.028216462202067305 1106 7 0.015508778673158474 1106 8 0.042874726268244956 1106 9 -0.01707716143735638 1106 10-11 0.013510280457616375 1106 12-13 0.022970472271403253 1106 14-15 0.004863421497375953 1106 16-17 0.0629136053917918 1106 18-19 -0.04305255219722426 1106 20-21 -0.07033387231061283 1106 22-23 0.006212489917786002 1106 24-25 0.06023059653442431 1106 26-27 0.08895925832316465 1106 28-29 0.08256447329268468 1106 30-31 0.02150312888762329 1106 32-33 0.008064959395319704 1106 34-35 0.07735627547746304 1106 36-37 0.06232650097116732 1106 38-39 0.0576083001285852 1106 40-41 0.24436300835077418 1106 42-43 0.08251283747394211 1106 44-45 0.06603765639098924 1106 46-47 0.1036888432089782 1106 48-49 0.08565934475941361 1106 50-51 3.810650091224943E-4 1106 52-53 -0.047803868235412494 1106 54-55 -0.03895837268068192 1106 56-57 0.05405422595754317 1106 58-59 0.027274629219604662 1106 60-61 -0.038263436828422925 1106 62-63 0.10128930471514508 1106 64-65 0.05282934951552676 1106 66-67 -0.022798617513281272 1106 68-69 -0.03213120078099507 1106 70-71 0.11911634662880743 1106 72-73 0.06681504073176825 1106 74-75 0.13776436188966912 1106 76-77 0.16541712290053567 1106 78-79 -0.006913740771167909 1106 80-81 -0.055714947495374645 1106 82-83 0.08789862883793731 1106 84-85 0.16574906782568988 1106 86-87 0.2766246671893988 1106 88-89 0.3032741416592195 1106 90-91 0.26434506250503276 1106 92-93 0.4453652807738777 1106 94-95 0.48903695501812194 1106 96-97 0.6560469106415319 1106 98-99 0.8146197875421564 1106 100-101 1.3648222658294138 1107 1 0.06434961309993525 1107 2 0.047793803834608184 1107 3 -0.007663443833308747 1107 4 -7.864843879801242E-4 1107 5 -0.043583034999329584 1107 6 -0.09333230011466043 1107 7 0.023609783458432787 1107 8 0.06678641286335818 1107 9 0.060487121566964674 1107 10-11 -0.015175513029021204 1107 12-13 0.03503630957206383 1107 14-15 0.04622590910584279 1107 16-17 0.07203331680668867 1107 18-19 0.09629256278398657 1107 20-21 0.020343868207568505 1107 22-23 0.08515070357845644 1107 24-25 0.08356263462080449 1107 26-27 0.012970995096345916 1107 28-29 0.07413746402809807 1107 30-31 0.05715464830409189 1107 32-33 -0.031487140128604096 1107 34-35 0.01029343662250426 1107 36-37 0.047810352199306294 1107 38-39 0.08823421037102719 1107 40-41 -0.17140361466504572 1107 42-43 -0.16793188097813072 1107 44-45 -0.17878773004134985 1107 46-47 -0.27101008836527285 1107 48-49 -0.13082155085795932 1107 50-51 -0.3306728886557124 1107 52-53 -0.29127184605915346 1107 54-55 -0.1880557793311155 1107 56-57 -0.14451860567668007 1107 58-59 -0.043220359092579486 1107 60-61 -0.0013059107297053174 1107 62-63 -0.033568238517553084 1107 64-65 -0.03005916337778558 1107 66-67 -0.08333723627008993 1107 68-69 -0.07814097326627945 1107 70-71 -0.11134556699148135 1107 72-73 -0.02131662163100856 1107 74-75 -0.04056697247986207 1107 76-77 -0.03871216518278686 1107 78-79 0.0204882284121517 1107 80-81 0.02372978931452252 1107 82-83 0.12058841821242439 1107 84-85 -0.12560944225276316 1107 86-87 0.007970204400724157 1107 88-89 -0.06737489945008335 1107 90-91 0.17227396262619266 1107 92-93 0.4157511929744935 1107 94-95 0.40085220792783716 1107 96-97 0.6811434422353599 1107 98-99 0.7861568887182813 1107 100-101 1.223213477323867 1108 1 0.13718777918236214 1108 2 0.056163428390732406 1108 3 0.027457355648017767 1108 4 0.011290886457274496 1108 5 -0.005414341814720558 1108 6 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-1.5058877258019692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 10.0 4 25.0 5 78.0 6 217.0 7 579.0 8 1243.0 9 2395.0 10 3925.0 11 5880.0 12 8325.0 13 11300.0 14 13924.0 15 18383.0 16 23530.0 17 30454.0 18 29685.0 19 22283.0 20 21141.0 21 22338.0 22 24372.0 23 27804.0 24 31755.0 25 38296.0 26 46530.0 27 56501.0 28 69688.0 29 83960.0 30 101883.0 31 124157.0 32 152936.0 33 192205.0 34 253241.0 35 353625.0 36 528449.0 37 816761.0 38 698838.0 39 68651.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.273941391438676 20.028908548788788 14.911429840659876 22.78572021911266 2 15.353890739129763 21.263402807447402 39.90858037051717 23.47412608290566 3 19.770601588519014 25.14474774668115 29.89746176693142 25.187188897868417 4 12.94861763842919 17.205215448681983 37.71700580123437 32.12916111165446 5 13.07135006743914 38.26305408801664 34.6246102522422 14.040985592302027 6 30.95246429661645 37.08001583442422 17.549236771486505 14.418283097472823 7 27.80698413025238 33.08474331982972 21.07294524602484 18.035327303893055 8 25.460135173393596 35.68955932238288 19.72661618683372 19.123689317389804 9 25.80061667069888 17.414847453788305 20.85883491447426 35.92570096103856 10-11 24.608777005080583 27.03496183127519 27.49334685410436 20.862914309539864 12-13 25.800436508140773 24.281254343204527 27.885200417977135 22.03310873067757 14-15 22.491969897410293 25.86049498375963 26.74172153045519 24.90581358837488 16-17 21.437186395265186 29.033888293333916 27.386741959686145 22.14218335171475 18-19 21.336149874658332 28.14188058825649 29.373356016657315 21.148613520427862 20-21 22.46883187744828 26.875003217188535 29.21110676433193 21.44505814103125 22-23 21.857977855447857 26.902979888711016 29.139788128831672 22.099254127009456 24-25 21.976518024184614 26.978611779645817 29.326217395903548 21.718652800266025 26-27 21.848191647923098 26.878532676607126 29.621051786294768 21.65222388917501 28-29 21.43543231292692 27.542840082565927 29.45179107320262 21.56993653130453 30-31 21.808047089345187 27.130615280873428 29.225455425209372 21.83588220457201 32-33 21.621591710463324 27.211443925690666 29.23086030195245 21.93610406189356 34-35 22.121278419401254 27.14422500263828 29.179238168288673 21.555258409671797 36-37 22.119523848626844 26.839824945408058 29.464060703302238 21.576590502662864 38-39 21.779979026303607 27.297801262643297 29.33722810516502 21.584991605888078 40-41 21.963979159633844 27.328289820238133 29.168945162058773 21.538785858069247 42-43 21.60883010775992 27.359921175195872 28.8271271197969 22.20412159724731 44-45 21.86555761477314 27.251517634493926 28.83389072053583 22.049034030197102 46-47 22.170922774921326 27.492222722390764 28.39364568043554 21.94320882225237 48-49 21.987851181216662 27.460533454679105 28.527074760457065 22.024540603647168 50-51 21.80016415387019 27.732944456641167 28.5899007535687 21.876990635919945 52-53 22.058164195007954 27.866772362034087 28.121856806798824 21.953206636159138 54-55 21.818200536369673 27.556815549573017 28.318607189000815 22.30637672505649 56-57 21.99391095603241 27.722844776221585 28.075684000804046 22.207560266941957 58-59 22.27020434016466 27.442878837852714 28.151680687462772 22.135236134519857 60-61 21.96293067115368 27.590300589081245 28.5880259826332 21.85874275713187 62-63 21.89137720784258 27.505267216320572 28.572969155448895 22.03038642038795 64-65 21.639746322085912 27.848564873863047 28.355073925046852 22.15661487900419 66-67 21.79416034346076 27.590819940491514 27.999979408827436 22.615040307220294 68-69 21.73241460463601 27.583789338927655 28.237420950236647 22.446375106199696 70-71 22.035492069620048 27.365801241836373 28.001441302319595 22.59726538622398 72-73 21.92497258955366 27.17979965923539 28.27906150749734 22.616166243713614 74-75 21.52506728457547 27.994681578014717 27.878090155282788 22.602160982127025 76-77 21.74528368698438 27.33711642735829 28.080266490517158 22.83733339514018 78-79 21.845427290375593 27.37713165787078 28.09301095611061 22.68443009564302 80-81 21.957702291472266 27.463731943184904 27.801617387201972 22.776948378140858 82-83 21.88382241474052 27.34982350344693 28.083490655269994 22.68286342654256 84-85 21.76379144382274 27.388646670338552 27.8865773746712 22.960984511167503 86-87 21.665866466288076 27.409596143785635 27.962256134801578 22.962281255124704 88-89 21.736949055357428 27.300444701006853 27.73569250220026 23.226913741435453 90-91 21.792525882072987 27.054746827348374 27.841524640064204 23.31120265051444 92-93 21.8905499526878 27.23090245270614 27.86206637400407 23.016481220601996 94-95 21.654565717035958 27.1536044186268 27.83767054360331 23.354159320733935 96-97 21.38957722495841 26.946761394521406 28.147983618573853 23.515677761946336 98-99 21.592534063592236 27.068497804590542 27.655982169054248 23.682985962762974 100-101 21.696365341378296 26.460979316946137 28.172442473040583 23.670212868634984 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2232.0 1 1456.5 2 846.5 3 1057.0 4 907.0 5 879.0 6 1750.0 7 3556.0 8 5076.0 9 5467.5 10 4569.0 11 3553.5 12 3490.5 13 3673.0 14 3880.5 15 4118.0 16 4385.0 17 4728.5 18 5068.0 19 5502.0 20 6261.0 21 7331.0 22 8787.0 23 11005.0 24 13882.0 25 17403.0 26 22607.0 27 29220.5 28 34572.5 29 40489.0 30 47711.0 31 55336.0 32 63770.0 33 71879.0 34 80707.0 35 89925.0 36 100347.5 37 109163.5 38 113735.0 39 116962.0 40 120645.5 41 124202.0 42 125813.0 43 130164.5 44 136164.5 45 139802.5 46 141754.0 47 152330.5 48 179489.0 49 176932.5 50 150435.5 51 138506.5 52 129953.5 53 123548.5 54 117363.5 55 108439.0 56 98394.5 57 87802.0 58 78699.5 59 70345.5 60 58791.0 61 46978.5 62 37075.0 63 28149.0 64 20724.0 65 15296.5 66 11096.0 67 8228.0 68 6090.5 69 4408.5 70 3190.0 71 2176.5 72 1619.0 73 1384.5 74 1042.0 75 740.5 76 509.0 77 285.0 78 188.0 79 138.5 80 107.5 81 69.5 82 39.5 83 36.5 84 26.5 85 15.0 86 8.5 87 3.0 88 2.0 89 1.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23210084985252408 2 0.0 3 0.0 4 0.0 5 0.02926354693749389 6 0.004478326444260278 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0029598134545398393 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.3753764110588924E-4 26-27 0.001209662890116282 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006949127241093535 36-37 0.016703642886924833 38-39 0.013229079266378063 40-41 0.008326083935162069 42-43 0.007940021310656874 44-45 0.0035903824078983267 46-47 5.919626909079678E-4 48-49 0.0016472005312221712 50-51 2.5737508300346425E-5 52-53 0.0 54-55 0.0 56-57 0.0010552378403142035 58-59 5.276189201571018E-4 60-61 0.002380719517782044 62-63 6.691752158090071E-4 64-65 0.0015313817438706125 66-67 0.005662251826076214 68-69 2.831125913038107E-4 70-71 1.2868754150173215E-4 72-73 0.0 74-75 4.3753764110588924E-4 76-77 0.05958233171530198 78-79 0.1424828459507178 80-81 0.0010166315778636838 82-83 9.008127905121249E-4 84-85 0.0 86-87 0.10450715245355667 88-89 0.35202476977798824 90-91 0.3870149123123092 92-93 4.7614390355640894E-4 94-95 2.573750830034643E-4 96-97 1.029500332013857E-4 98-99 0.0 100-101 0.0012740066608671482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3885380.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.05798926648859 #Duplication Level Percentage of deduplicated Percentage of total 1 67.79548447056827 31.903191805323956 2 16.276241897356144 15.318544330091147 3 6.504507884968118 9.182671867038604 4 3.2265279009116514 6.073356613165058 5 1.8220384107027945 4.287073198699102 6 1.0932943469955514 3.086894018761759 7 0.7258680831291487 2.3910524725344695 8 0.4834624317580102 1.8200615939535134 9 0.35955797009829593 1.5228067587809435 >10 1.5538260305123042 12.716091610553512 >50 0.10256027907550841 3.3237549734632617 >100 0.05219742433971386 4.423837800473391 >500 0.00246270536012203 0.796318555722499 >1k 0.0016965303043894294 1.77651580627565 >5k 1.0945356802512428E-4 0.37975851786467074 >10k+ 1.6418035203768643E-4 0.9980700772986107 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14233 0.3663219556388307 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13266 0.3414337851123957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11256 0.2897013934286994 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7775 0.20010912703519348 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6971 0.17941617036171495 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4917 0.12655132831280339 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4823 0.12413200253257081 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3908 0.10058218243775383 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.3163757470311784E-4 2 0.0 0.0 0.0 5.147501660069286E-5 2.3163757470311784E-4 3 0.0 0.0 0.0 5.147501660069286E-5 2.3163757470311784E-4 4 0.0 0.0 0.0 5.147501660069286E-5 2.3163757470311784E-4 5 0.0 0.0 0.0 5.147501660069286E-5 2.3163757470311784E-4 6 0.0 0.0 0.0 5.147501660069286E-5 2.831125913038107E-4 7 0.0 0.0 0.0 5.147501660069286E-5 2.831125913038107E-4 8 0.0 0.0 0.0 5.147501660069286E-5 2.831125913038107E-4 9 0.0 0.0 0.0 7.721252490103928E-5 2.831125913038107E-4 10-11 3.860626245051964E-5 0.0 0.0 7.721252490103928E-5 2.831125913038107E-4 12-13 1.0295003320138571E-4 0.0 0.0 7.721252490103928E-5 9.394190529626445E-4 14-15 1.0295003320138571E-4 0.0 0.0 7.721252490103928E-5 0.0011067128569148964 16-17 1.1581878735155892E-4 0.0 0.0 9.00812790512125E-5 0.0012096628901162821 18-19 1.4155629565190534E-4 0.0 0.0 1.1581878735155892E-4 0.0012354003984166285 20-21 1.5442504980207856E-4 0.0 0.0 2.0590006640277143E-4 0.0013640879399183605 22-23 1.5442504980207856E-4 0.0 0.0 3.4745636205467677E-4 0.0014284317106692266 24-25 2.0590006640277143E-4 0.0 0.0 5.790939367577945E-4 0.0014413004648194 26-27 2.0590006640277143E-4 0.0 0.0 0.0011195816110650696 0.0015185129897204394 28-29 2.44506328853291E-4 0.0 0.0 0.0036547261786491923 0.001569988006321132 30-31 2.5737508300346424E-4 0.0 0.0 0.011118603585749657 0.001569988006321132 32-33 2.5737508300346424E-4 2.573750830034643E-5 0.0 0.025557345742244005 0.0016600692853723446 34-35 2.831125913038107E-4 2.573750830034643E-5 0.0 0.05146214784654268 0.001750150564423557 36-37 3.3458760790450355E-4 2.573750830034643E-5 0.0 0.0927708486685987 0.001750150564423557 38-39 4.1180013280554286E-4 2.573750830034643E-5 0.0 0.15071884860682866 0.0017630193185737301 40-41 4.1180013280554286E-4 2.573750830034643E-5 0.0 0.21799669530393423 0.0017758880727239035 42-43 4.1180013280554286E-4 2.573750830034643E-5 0.0 0.28826009296388 0.0018016255810242498 44-45 4.3753764110588924E-4 2.573750830034643E-5 0.0 0.3727563327139173 0.0018659693517751159 46-47 4.3753764110588924E-4 2.573750830034643E-5 0.0 0.4805964924923688 0.0019431818766761553 48-49 4.632751494062357E-4 2.573750830034643E-5 0.0 0.5981010866376004 0.0020332631557273676 50-51 4.632751494062357E-4 2.573750830034643E-5 0.0 0.7235457020934889 0.002110475680628407 52-53 4.632751494062357E-4 2.573750830034643E-5 0.0 0.8469055793770494 0.002213425713829793 54-55 4.632751494062357E-4 2.573750830034643E-5 0.0 0.9881272874210502 0.002290638238730832 56-57 5.147501660069285E-4 2.573750830034643E-5 0.0 1.1593331926349548 0.002303506992881005 58-59 5.147501660069285E-4 2.573750830034643E-5 0.0 1.3470111031610807 0.0023163757470311785 60-61 5.147501660069285E-4 2.573750830034643E-5 0.0 1.5507749563749234 0.002342113255331525 62-63 5.147501660069285E-4 2.573750830034643E-5 0.0 1.756623547761094 0.0024450632885329106 64-65 5.147501660069285E-4 2.573750830034643E-5 0.0 1.971235760723533 0.0024965383051336032 66-67 5.404876743072749E-4 2.573750830034643E-5 0.0 2.2134257138297926 0.0025094070592837766 68-69 5.404876743072749E-4 2.573750830034643E-5 0.0 2.495097004668784 0.00252227581343395 70-71 5.790939367577945E-4 2.573750830034643E-5 0.0 2.801540647246859 0.0025737508300346427 72-73 6.177001992083142E-4 5.147501660069286E-5 0.0 3.1068647082138683 0.0027024383715363747 74-75 6.177001992083142E-4 5.147501660069286E-5 0.0 3.4258039110717613 0.0028053884047377605 76-77 6.177001992083142E-4 5.147501660069286E-5 0.0 3.771098322429209 0.0028053884047377605 78-79 6.177001992083142E-4 5.147501660069286E-5 0.0 4.164251630471151 0.002895469683788973 80-81 7.206502324097E-4 5.147501660069286E-5 0.0 4.590336080383386 0.0029598134545398393 82-83 7.206502324097E-4 5.147501660069286E-5 0.0 5.024502107901929 0.0031657135209426104 84-85 7.721252490103928E-4 5.147501660069286E-5 0.0 5.462605974190427 0.0032171885375433034 86-87 8.236002656110857E-4 5.147501660069286E-5 0.0 5.918623146255965 0.003307269816594516 88-89 8.236002656110857E-4 5.147501660069286E-5 0.0 6.414134524808384 0.003397351095645728 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 1250 0.0 22.073816 1 GTACATG 17770 0.0 21.791967 1 TACATGG 17780 0.0 21.568241 2 ACATGGG 18120 0.0 20.115839 3 GAGTACT 12990 0.0 19.888958 12-13 TACCTGG 2980 0.0 18.633942 2 GTACTTT 13960 0.0 18.35404 14-15 AGTACTT 14060 0.0 18.122257 12-13 CATGGGG 7375 0.0 17.18242 4 CATGGGA 10495 0.0 16.732252 4 ACTTTTT 16260 0.0 15.946896 16-17 GTATAGA 1360 0.0 15.741026 1 GTACCTG 3785 0.0 15.710991 1 AGTACAT 14045 0.0 15.645678 2 AGAGTAC 27065 0.0 15.637962 10-11 GTGTAGG 1540 0.0 15.44574 1 GTATCAA 35970 0.0 15.421178 1 TACTTTT 16355 0.0 15.390693 14-15 CCGATCG 355 5.2832183E-7 14.701245 9 GTGTAGC 2565 0.0 14.652076 1 >>END_MODULE