FastQCFastQC Report
Thu 26 May 2016
SRR938789_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938789_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915664
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52470.5730267871184189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44280.48358349787695054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26170.2858035261842772No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17090.18664051442450505No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.10047353614426252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41500.038.380351
GTATCAA63900.031.8996071
TATCAAC76850.026.3233092
ATCAACG77250.026.125683
TCAACGC77200.026.0812324
CAACGCA77550.025.9635225
AACGCAG79650.025.261036
GAGTACT46800.023.06727412-13
ACGCAGA88450.022.6432067
CGCAGAG88550.022.3502868
AGTACTT48350.021.20160112-13
GCAGAGT90650.020.8401349
GTACTTT52050.020.65076414-15
GCGGTAT1404.91711E-420.3161281
GTACATG42300.020.1720431
AGAGTAC72300.020.07185710-11
TACATGG43300.019.47552
ACATGGG42350.019.353043
AGTACGG1250.006122100618.9503172
CAGAGTA87850.018.7826310-11
Produced by FastQC (version 0.11.5)