FastQCFastQC Report
Thu 26 May 2016
SRR938789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915664
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47910.5232268605077846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43870.4791058728966084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27980.3055706023170071No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT22800.24899963305317235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17570.1918826119624666No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT10190.11128536231630817RNA PCR Primer, Index 31 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.10516958185535305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.040.878041
TAGCGTA704.342741E-640.668427
ATAGCGT905.746788E-736.902826
GTATCAA56300.031.2165781
GCGTAAG856.305719E-428.016791
AATAGCG909.005223E-426.3591585
TATCAAC68250.025.7219052
TCAACGC69400.025.3640444
ATCAACG69450.025.3457853
CAACGCA70750.024.8800665
AACGCAG72200.024.3803986
TAGTACA2453.0195224E-1023.2390944
GAGTACT46750.022.32897812-13
GTACATG39650.021.862281
ACGCAGA81000.021.614517
CGCAGAG80650.021.5906528
GTCTTAG3101.0913936E-1121.5096631
TACATGG40200.021.2446942
ACATGGG39200.021.0604293
GAATAGG2308.761708E-820.7080611
Produced by FastQC (version 0.11.5)