##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938789_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 915664 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9750618130668 33.0 31.0 34.0 30.0 34.0 2 32.19235112442992 34.0 31.0 34.0 30.0 34.0 3 32.27012965454577 34.0 31.0 34.0 30.0 34.0 4 35.92438383511856 37.0 35.0 37.0 35.0 37.0 5 35.83490778276748 37.0 35.0 37.0 35.0 37.0 6 35.858721102937324 37.0 35.0 37.0 35.0 37.0 7 35.79170962274372 37.0 35.0 37.0 33.0 37.0 8 35.769806391864265 37.0 35.0 37.0 33.0 37.0 9 37.40716354470636 39.0 37.0 39.0 34.0 39.0 10-11 37.346093108389105 39.0 37.0 39.0 34.0 39.0 12-13 37.28190908455503 39.0 37.0 39.0 33.0 39.0 14-15 38.49655168271331 40.0 38.0 41.0 34.0 41.0 16-17 38.43235564573905 40.0 38.0 41.0 33.0 41.0 18-19 38.46759510038616 40.0 38.0 41.0 33.5 41.0 20-21 38.519581964563415 40.0 38.0 41.0 34.0 41.0 22-23 38.46316825822572 40.0 38.0 41.0 34.0 41.0 24-25 38.38218385783431 40.0 38.0 41.0 34.0 41.0 26-27 38.20508068461794 40.0 38.0 41.0 33.0 41.0 28-29 38.03763716821891 40.0 37.0 41.0 33.0 41.0 30-31 37.77479948976917 40.0 37.0 41.0 33.0 41.0 32-33 37.5081427248423 40.0 36.0 41.0 32.0 41.0 34-35 37.411641169686696 40.0 36.0 41.0 32.0 41.0 36-37 37.29361479756767 40.0 36.0 41.0 31.5 41.0 38-39 37.16125456499327 40.0 36.0 41.0 31.0 41.0 40-41 36.974052709290746 40.0 36.0 41.0 31.0 41.0 42-43 36.7648771820231 40.0 35.5 41.0 31.0 41.0 44-45 36.46493692009296 40.0 35.0 41.0 30.0 41.0 46-47 36.08020409233081 39.0 35.0 41.0 29.5 41.0 48-49 35.73061461409425 39.0 35.0 41.0 27.5 41.0 50-51 35.46354066557165 39.0 35.0 41.0 27.0 41.0 52-53 35.25258391724475 39.0 35.0 41.0 26.5 41.0 54-55 35.01642469290045 39.0 35.0 40.0 26.0 41.0 56-57 34.74699398469308 38.0 34.0 40.0 25.5 41.0 58-59 34.439263747400794 38.0 34.0 40.0 24.0 41.0 60-61 34.0921669957539 38.0 34.0 40.0 23.0 41.0 62-63 33.72822672945534 37.0 33.5 40.0 22.0 41.0 64-65 33.36050068584109 37.0 33.0 40.0 21.5 41.0 66-67 32.95130091387234 36.0 33.0 39.0 19.5 41.0 68-69 32.49575117073512 36.0 32.0 39.0 18.0 40.0 70-71 32.037920023065226 35.0 31.5 38.5 14.5 40.0 72-73 31.713506810358382 35.0 31.0 38.0 12.0 40.0 74-75 31.29866250065526 35.0 31.0 37.0 7.5 39.0 76-77 29.213913618969404 33.0 28.5 35.0 6.0 37.5 78-79 30.255759754669835 34.0 30.0 36.0 2.0 39.0 80-81 30.28439143615999 34.0 31.0 36.0 2.0 37.5 82-83 29.98468488441175 34.0 30.5 35.0 2.0 37.0 84-85 29.596391252686576 34.0 30.0 35.0 2.0 37.0 86-87 29.07241739327963 34.0 29.5 35.0 2.0 36.0 88-89 28.57573356602422 34.0 29.0 35.0 2.0 36.0 90-91 28.18103420031802 34.0 29.0 35.0 2.0 35.0 92-93 27.905362665781336 34.0 28.5 35.0 2.0 35.0 94-95 27.543509409565083 33.5 27.0 35.0 2.0 35.0 96-97 27.08119190008562 33.0 26.5 35.0 2.0 35.0 98-99 26.415281697216447 33.0 25.0 35.0 2.0 35.0 100-101 23.669886006220622 30.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05850244078306588 1101 2 0.053340332491004006 1101 3 0.07587898670958992 1101 4 0.026515735285443043 1101 5 -0.005757096719584354 1101 6 0.1715804111817789 1101 7 0.17705268059677337 1101 8 0.09205924064618642 1101 9 0.21063827481262365 1101 10-11 0.10441708622513346 1101 12-13 0.27769807313637784 1101 14-15 0.39134673922358587 1101 16-17 0.2291055726699227 1101 18-19 0.19118433032765836 1101 20-21 0.02600276723529049 1101 22-23 0.10632202492306675 1101 24-25 0.006112829476833781 1101 26-27 0.11509704588404901 1101 28-29 -0.05200941412136473 1101 30-31 -0.04579243010051215 1101 32-33 0.06259367753262524 1101 34-35 0.01637744918152606 1101 36-37 -0.11971418499987863 1101 38-39 -0.12861926523031286 1101 40-41 -0.11009649255385767 1101 42-43 -0.19041792209431208 1101 44-45 -0.07451308530933431 1101 46-47 -0.22588965402117367 1101 48-49 -0.25906831987379775 1101 50-51 -0.19344989479228758 1101 52-53 -0.013270239903334868 1101 54-55 -0.32383643590414835 1101 56-57 -0.10706308407082332 1101 58-59 -0.2553629348314459 1101 60-61 -0.025126283278446238 1101 62-63 0.012348622655252939 1101 64-65 -0.21365206999666952 1101 66-67 -0.355433228876457 1101 68-69 -0.26062051675830133 1101 70-71 -0.25348306569496515 1101 72-73 -0.12954453125696475 1101 74-75 0.0711481796268778 1101 76-77 -0.16099329094623016 1101 78-79 0.03613996071424097 1101 80-81 0.09885115433571912 1101 82-83 0.1664393853595314 1101 84-85 0.1296467804493595 1101 86-87 0.24203163922573978 1101 88-89 0.2926229895413677 1101 90-91 0.430466347427064 1101 92-93 0.13290445131145745 1101 94-95 0.2081126443771204 1101 96-97 0.2804065285666155 1101 98-99 0.34418467080236326 1101 100-101 0.4382672116542814 1102 1 0.12763027538019145 1102 2 0.08839641388199482 1102 3 0.08355406608129101 1102 4 0.10934375036755739 1102 5 0.008007117217672999 1102 6 0.048648096029971555 1102 7 -0.021031081115395978 1102 8 -0.07038022540417188 1102 9 0.015114362041082074 1102 10-11 0.05968674151667841 1102 12-13 0.1570851091916765 1102 14-15 0.26532964973758055 1102 16-17 0.20000311318969466 1102 18-19 0.1317069326471625 1102 20-21 0.2698250282416055 1102 22-23 0.37132893457931715 1102 24-25 0.28885774998600766 1102 26-27 0.0617400549202074 1102 28-29 0.16563358895820102 1102 30-31 0.14258356241798964 1102 32-33 0.09226797063309533 1102 34-35 0.15067421296063088 1102 36-37 0.18230182634149372 1102 38-39 0.24744175418224756 1102 40-41 0.32734505830656957 1102 42-43 0.11901629023466143 1102 44-45 0.17803708415593889 1102 46-47 0.07918060407831007 1102 48-49 0.296174679631811 1102 50-51 0.26586578119248827 1102 52-53 0.2534238767626391 1102 54-55 0.23337447539611134 1102 56-57 0.2826273811992195 1102 58-59 0.1201664580122852 1102 60-61 0.36268040717491346 1102 62-63 0.23497432181120104 1102 64-65 0.17517692196788204 1102 66-67 0.16314615175625846 1102 68-69 -0.07148207941279594 1102 70-71 -0.4180535054707555 1102 72-73 -0.08280606770242471 1102 74-75 -0.06438606784603351 1102 76-77 -0.3300296917301182 1102 78-79 -0.3227399899651111 1102 80-81 -0.11764296947817243 1102 82-83 -0.1582653161850125 1102 84-85 -0.23099409016732864 1102 86-87 -0.16153100336037696 1102 88-89 0.01149305226436681 1102 90-91 -0.050852265161612564 1102 92-93 0.03307108720235519 1102 94-95 -0.09557195959831333 1102 96-97 -0.017036302962861072 1102 98-99 0.0686591497487612 1102 100-101 0.4854539093746908 1103 1 0.10778960392418213 1103 2 -0.08566575270169352 1103 3 -0.02449771569551018 1103 4 -0.11788009201099214 1103 5 -0.0821842192927491 1103 6 -0.03275456418767675 1103 7 -0.14122028376138474 1103 8 0.0017202084815934882 1103 9 -0.04179580226648483 1103 10-11 -0.06759827164214727 1103 12-13 0.020452347259819703 1103 14-15 -0.08894448071266225 1103 16-17 -0.09031198745757507 1103 18-19 0.07611478526825977 1103 20-21 -0.14891030144045203 1103 22-23 -0.19414015995087652 1103 24-25 -0.09227604331366024 1103 26-27 -0.1648870167065084 1103 28-29 -0.016154589432872513 1103 30-31 -0.0015646700309659423 1103 32-33 -0.11528519815558269 1103 34-35 -0.10076685681674036 1103 36-37 -0.08405495579095401 1103 38-39 -0.021592316519793542 1103 40-41 0.05927905076691076 1103 42-43 -0.005768545102135647 1103 44-45 -0.16724707545712647 1103 46-47 -0.47438863982239354 1103 48-49 -0.3923572216413973 1103 50-51 -0.2990613117728742 1103 52-53 -0.19737774497433236 1103 54-55 -0.4480395654318272 1103 56-57 -0.5719326870844412 1103 58-59 -0.7335286838896948 1103 60-61 -0.6265997692245477 1103 62-63 -0.5957113599584787 1103 64-65 -0.6579783522336982 1103 66-67 -0.7667763178361682 1103 68-69 -0.7292585926783204 1103 70-71 -0.8595450767062651 1103 72-73 -0.9091678288518672 1103 74-75 -0.5534635271247872 1103 76-77 -0.7995531286744253 1103 78-79 -0.8993236730151146 1103 80-81 -0.8056229111525433 1103 82-83 -0.8734562970513622 1103 84-85 -1.0838826313153476 1103 86-87 -0.6894867612378945 1103 88-89 -0.4117134346834952 1103 90-91 -0.38623315996209584 1103 92-93 -0.5608495330409049 1103 94-95 -0.5342475410763683 1103 96-97 -0.6956670732879218 1103 98-99 -0.68527925269807 1103 100-101 -0.10174148147814321 1104 1 -0.09980645183916081 1104 2 0.0034399936430915545 1104 3 -0.066286223005946 1104 4 -0.10208274417011864 1104 5 0.012069435926562733 1104 6 0.032447747951046324 1104 7 -0.1308632623400996 1104 8 -0.1554848238203448 1104 9 -0.2393816574177876 1104 10-11 -0.25073325974143046 1104 12-13 -0.08635486117269409 1104 14-15 -0.038091029505594065 1104 16-17 -0.15074721357113674 1104 18-19 -0.10765604098131121 1104 20-21 -0.1832863640042035 1104 22-23 -0.1494376778019486 1104 24-25 -0.14741375157615266 1104 26-27 -0.24399276305128836 1104 28-29 -0.3456887614831885 1104 30-31 -0.34361839325436705 1104 32-33 -0.07631545997129052 1104 34-35 -0.2680262923828778 1104 36-37 -0.29708113736185027 1104 38-39 -0.1003240404232244 1104 40-41 -0.08381513488422598 1104 42-43 -0.05243634517013618 1104 44-45 -0.023605456960012816 1104 46-47 -0.257023797931879 1104 48-49 -0.34647826890423516 1104 50-51 -0.4072252016062663 1104 52-53 -0.41492959808552143 1104 54-55 -0.5495696572373348 1104 56-57 -0.43985516243296274 1104 58-59 -0.3933722745051327 1104 60-61 -0.34424342784407 1104 62-63 -0.5150959230322698 1104 64-65 -0.2571385018426753 1104 66-67 -0.12396094891403919 1104 68-69 -0.14434869808464867 1104 70-71 -0.20579805188477707 1104 72-73 -0.5110787435067472 1104 74-75 -0.44909086476504356 1104 76-77 -0.3246568348967962 1104 78-79 -0.42159160909127635 1104 80-81 -0.7454767023800173 1104 82-83 -0.7404301154804678 1104 84-85 -0.5035902137702948 1104 86-87 -0.17188390506652595 1104 88-89 -0.1255046621571445 1104 90-91 -0.14091524088695095 1104 92-93 -0.4170991080154032 1104 94-95 -0.2625848413143821 1104 96-97 -0.3764117179666009 1104 98-99 -0.06899907588746146 1104 100-101 0.5949331189978864 1105 1 0.07417929266681256 1105 2 -0.018096851015144466 1105 3 -0.022413525376023813 1105 4 -0.04512813808256766 1105 5 -0.13826539302097984 1105 6 0.016522825089708704 1105 7 -0.062380300215522766 1105 8 0.06536754154069513 1105 9 -0.05362906704092296 1105 10-11 0.10273629575124232 1105 12-13 0.01508281483145879 1105 14-15 -0.018982873692237945 1105 16-17 0.060254308991076755 1105 18-19 -0.03229190607960675 1105 20-21 -0.011449722803398288 1105 22-23 -0.1286513350502858 1105 24-25 -0.046825611392634414 1105 26-27 -0.02851652445366426 1105 28-29 -0.12313799818097948 1105 30-31 -0.18085851344246606 1105 32-33 -0.15590908407359194 1105 34-35 -0.05736633576916006 1105 36-37 -0.3538794450272107 1105 38-39 -0.31749935066211776 1105 40-41 -0.43479334742313114 1105 42-43 -0.542283011028367 1105 44-45 -0.10699066520071199 1105 46-47 0.07943146164476644 1105 48-49 0.0381775377168907 1105 50-51 0.05800985235711664 1105 52-53 0.006432758247939319 1105 54-55 0.2044011311265166 1105 56-57 0.0691299757429249 1105 58-59 -0.16562795716544088 1105 60-61 -0.1351929407588912 1105 62-63 -0.1571442408031274 1105 64-65 -0.1262136463513457 1105 66-67 0.06879340655708432 1105 68-69 0.06220034054531709 1105 70-71 0.05641680454973397 1105 72-73 -0.24220773012705976 1105 74-75 -0.10153976461279157 1105 76-77 -0.149298089595856 1105 78-79 -0.24323152986414698 1105 80-81 0.05103689962491487 1105 82-83 -0.024424348607649193 1105 84-85 0.286406687209265 1105 86-87 0.15335020571671976 1105 88-89 0.3452068998838982 1105 90-91 0.3759346722057373 1105 92-93 0.485168566506605 1105 94-95 0.479420359650355 1105 96-97 0.5521376709874133 1105 98-99 0.7260384492078664 1105 100-101 0.8356313417770309 1106 1 -0.21400634771282157 1106 2 -0.2785661738401224 1106 3 -0.24207684896544635 1106 4 -0.05801594311817837 1106 5 -0.1552041894055094 1106 6 -0.23765063809068465 1106 7 -0.20208701382523486 1106 8 -0.1585351915999169 1106 9 -0.2315254585631834 1106 10-11 -0.1988983667379216 1106 12-13 -0.1616655715012172 1106 14-15 -0.057704160104051994 1106 16-17 -0.2192129520004329 1106 18-19 -0.2437765338459883 1106 20-21 -0.269138531300527 1106 22-23 -0.3022477540277677 1106 24-25 -0.2602980511930255 1106 26-27 -0.2791921382932472 1106 28-29 -0.2189069328644635 1106 30-31 -0.0987566276454217 1106 32-33 -0.2564059697898742 1106 34-35 -0.15333580125363966 1106 36-37 -0.17818754647156965 1106 38-39 -0.4254912432960438 1106 40-41 -0.38625008929557936 1106 42-43 0.041392166757667326 1106 44-45 -0.05535331102294094 1106 46-47 -0.06751185677661198 1106 48-49 -0.040974257370244516 1106 50-51 0.17003120283111883 1106 52-53 0.01941073485177469 1106 54-55 -0.036852226755016204 1106 56-57 -0.09437664850273109 1106 58-59 -0.24332354871784645 1106 60-61 -0.2614102569300272 1106 62-63 -0.0015067681910565511 1106 64-65 -0.05692958168621942 1106 66-67 -0.01811852677517578 1106 68-69 0.02725818493341592 1106 70-71 0.08767269261855404 1106 72-73 0.16993555384070191 1106 74-75 0.16766947110914998 1106 76-77 0.18986678709789118 1106 78-79 0.21944551595377604 1106 80-81 0.04660368849424046 1106 82-83 0.2904453467470347 1106 84-85 -0.05453335811877125 1106 86-87 0.3016312933742533 1106 88-89 0.44322339455166215 1106 90-91 0.6332587568970283 1106 92-93 0.511860696547469 1106 94-95 0.8271313476615738 1106 96-97 0.9466342444678162 1106 98-99 0.9640523598545698 1106 100-101 1.3907894246487373 1107 1 0.005271905378684494 1107 2 0.06399829672552215 1107 3 0.00537742911077288 1107 4 -0.059489050692931755 1107 5 -0.03288572527807787 1107 6 -0.08391430160562408 1107 7 0.027442349782184294 1107 8 0.01908923924488448 1107 9 -0.011405790681976669 1107 10-11 0.044739223530974925 1107 12-13 -0.16812147176450765 1107 14-15 -0.10347788269489655 1107 16-17 -0.011004483274284382 1107 18-19 0.03497306818755419 1107 20-21 -0.02798096690576557 1107 22-23 -0.06720876594907566 1107 24-25 0.03187249575185547 1107 26-27 0.15135703014636448 1107 28-29 0.2548146472771293 1107 30-31 0.046386160191708825 1107 32-33 0.1419988795893019 1107 34-35 0.2388405572648722 1107 36-37 0.2955125561153409 1107 38-39 0.33387170716407866 1107 40-41 0.33081850243379307 1107 42-43 0.28784710684792003 1107 44-45 0.2983984387660712 1107 46-47 0.19401527316512102 1107 48-49 -0.11424935705135653 1107 50-51 0.07395452038055339 1107 52-53 0.21176371467298338 1107 54-55 0.2098958187875084 1107 56-57 0.11871733222305636 1107 58-59 -0.07699631164101817 1107 60-61 -0.1397108517319552 1107 62-63 -0.09809133164080919 1107 64-65 -0.16943027654828313 1107 66-67 -0.08696166987633092 1107 68-69 0.16578967608213446 1107 70-71 0.07772226786833158 1107 72-73 -0.08694231720300749 1107 74-75 0.10846722329354108 1107 76-77 0.23420025013880874 1107 78-79 0.052991940319937925 1107 80-81 -0.0719107216815722 1107 82-83 0.08907684749748412 1107 84-85 -0.035561097012347886 1107 86-87 0.16306569081513445 1107 88-89 0.11235787436529066 1107 90-91 0.24070338270748692 1107 92-93 0.19392971782793467 1107 94-95 0.14662387094577056 1107 96-97 0.28073529509925876 1107 98-99 0.33308475811413274 1107 100-101 0.7687476419611627 1108 1 -0.013132494644970905 1108 2 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quality scores pass #Quality Count 3 4.0 4 8.0 5 48.0 6 110.0 7 273.0 8 503.0 9 900.0 10 1514.0 11 2334.0 12 3333.0 13 4549.0 14 4926.0 15 6465.0 16 8257.0 17 10956.0 18 10598.0 19 6792.0 20 5852.0 21 5957.0 22 6501.0 23 6969.0 24 7863.0 25 9127.0 26 10464.0 27 12618.0 28 15358.0 29 18428.0 30 22400.0 31 27168.0 32 33171.0 33 41630.0 34 54787.0 35 75026.0 36 111657.0 37 182669.0 38 184070.0 39 22378.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.1419744288593 21.25033712595464 15.34389040062623 22.26379804455983 2 15.45730748396792 22.620852190323088 39.57587062503276 22.34596970067623 3 18.119528560694754 26.66775148962938 32.30082213563053 22.911897814045325 4 12.75882856593685 19.04344825176047 37.71241416065282 30.485309021649865 5 12.361739677436265 38.42883415750756 35.0412378339653 14.168188331090882 6 28.61912229813556 37.954970382148915 18.42640968739625 14.999497632319278 7 25.04630519491866 34.9011209351902 22.636360062206222 17.416213807684915 8 24.520457285641896 35.53705289276416 21.455686802145767 18.486803019448182 9 25.35537052892764 17.201615439724616 22.318667109332683 35.124346922015064 10-11 23.34535375421552 27.619792849778957 28.840273288018313 20.19458010798721 12-13 24.611920966642785 25.841629680756263 28.867084432717675 20.679364919883277 14-15 21.762822000464144 27.519582815720856 27.588713090248863 23.128882093566133 16-17 20.204412947573108 30.220778433598554 28.69600877631269 20.878799842515644 18-19 20.11434343985912 28.276957940289133 31.590351248413036 20.018347371438715 20-21 21.119123520334444 27.53938949154875 31.30671436980636 20.03477261831045 22-23 20.771210837162975 28.047569850949692 30.978939873141236 20.20227943874609 24-25 20.678272816229534 28.010765957818588 31.371824162574917 19.93913706337696 26-27 20.3428118984888 28.173426344313548 31.446793987959538 20.03696776923812 28-29 20.240721487357806 28.262659665554178 31.229523056492337 20.267095790595675 30-31 20.420263328032988 27.82734714917262 31.32355318107952 20.428836341714867 32-33 20.065165825018784 28.29951816386797 31.1259916301176 20.50932438099565 34-35 20.65480702229581 28.09906749468653 30.990838774221874 20.25528670879578 36-37 20.546555166703804 28.01911708806837 30.931026874462052 20.503300870765777 38-39 20.439269260763286 28.032659578883447 30.985969334815838 20.542101825537426 40-41 20.631828765896472 27.736912578690536 31.15014875296523 20.481109902447763 42-43 20.398586990769786 28.034384899214697 30.822888390509533 20.744139719505984 44-45 20.337236579995807 28.112659013070523 30.570372020689174 20.979732386244496 46-47 20.72128154050465 27.935680523519956 30.10141451736912 21.241623418606277 48-49 20.67324913942232 28.174199269601075 29.747593003547152 21.40495858742945 50-51 20.31145823151703 28.421341752756618 29.768019915678355 21.499180100047997 52-53 20.462855370528928 28.567793426409686 29.66328260147827 21.306068601583114 54-55 20.243943193136346 28.28461094899439 29.585470216149158 21.88597564172011 56-57 20.489557304862917 28.531153348826642 29.002123049502877 21.977166296807564 58-59 20.37201662629497 28.337490635149802 29.34212336920553 21.94836936934969 60-61 20.29551426405929 28.384942849841316 29.505230118668763 21.814312767430636 62-63 20.06183490887487 28.463661343025386 29.457857904209405 22.016645843890334 64-65 19.99614487410229 28.545787537786786 29.132356410211607 22.325711177899315 66-67 20.022617763080298 28.68415074722711 28.7692267367952 22.52400475289739 68-69 20.122556875859015 28.19108475722064 28.994879630079623 22.691478736840725 70-71 20.210135565343414 28.021770398417733 28.797724450579754 22.970369585659103 72-73 20.130855859791364 27.8850102220902 28.712934001991997 23.271199916126438 74-75 19.84821943420294 28.125491446644187 28.587178266263606 23.439110852889268 76-77 19.77724053529175 27.810716220271363 28.915604039776838 23.496439204660053 78-79 19.80971244379891 27.629823078893732 28.754473008456316 23.805991468851037 80-81 19.780792834922437 27.60277110532417 28.641471457582394 23.974964602170996 82-83 19.619205298013245 27.531387059008544 28.637961086162612 24.2114465568156 84-85 19.573500760104142 27.224232906393613 28.578004595572175 24.62426173793007 86-87 19.47498546700011 27.238040968020837 28.48861722949343 24.79835633548562 88-89 19.571842658723746 26.861430394163992 28.46056963103656 25.106157316075706 90-91 19.420991389341403 26.746978055825544 28.60496379142767 25.227066763405386 92-93 19.455062118855825 26.68811922277167 28.478022506072097 25.37879615230041 94-95 19.257729504343025 26.318183481158748 28.64163527321991 25.78245174127832 96-97 19.341821531599763 26.132744179493887 28.519701833697642 26.005732455208708 98-99 19.270200537861932 26.0073444091027 28.479434289380638 26.24302076365473 100-101 19.187097351956027 25.715524481328707 28.922948221877842 26.174429944837424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1130.0 1 754.0 2 434.5 3 512.0 4 477.0 5 486.0 6 692.5 7 1136.5 8 1651.0 9 1863.5 10 1665.0 11 1473.5 12 1494.5 13 1557.0 14 1644.5 15 1767.0 16 1895.0 17 2007.5 18 2159.0 19 2310.0 20 2549.0 21 2895.5 22 3360.0 23 3881.0 24 4470.5 25 5428.5 26 6769.5 27 8413.5 28 9815.0 29 11379.5 30 13439.0 31 15478.0 32 17759.5 33 20066.0 34 22609.0 35 25176.0 36 27947.5 37 30268.5 38 30627.0 39 29805.0 40 29325.5 41 29801.5 42 30307.0 43 31138.5 44 32382.5 45 33043.5 46 33188.5 47 33528.5 48 34524.0 49 34238.5 50 32478.5 51 30944.5 52 28995.5 53 26939.0 54 24788.5 55 22213.0 56 19619.5 57 16847.5 58 14715.5 59 12783.0 60 10538.5 61 8537.0 62 6861.5 63 5132.0 64 3583.5 65 2696.0 66 2008.0 67 1505.5 68 1127.0 69 774.0 70 553.0 71 446.0 72 361.0 73 267.0 74 213.5 75 178.0 76 123.0 77 79.0 78 59.0 79 38.0 80 23.0 81 22.5 82 18.0 83 10.0 84 7.5 85 7.5 86 5.0 87 2.0 88 0.0 89 1.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3868231141554107 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.638155480612976E-4 16-17 0.0019111813940484719 18-19 1.638155480612976E-4 20-21 1.092103653741984E-4 22-23 0.0 24-25 0.0 26-27 1.638155480612976E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007207884114697095 36-37 0.016163134075381363 38-39 0.010702615806671442 40-41 0.005678938999458316 42-43 0.003221705778538853 44-45 0.0017473658459871745 46-47 0.0017473658459871745 48-49 0.0 50-51 3.822362788096944E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.552621922451904E-4 60-61 6.552621922451904E-4 62-63 0.0 64-65 0.0 66-67 0.0013105243844903807 68-69 8.19077740306488E-4 70-71 0.0018565762113613726 72-73 0.0 74-75 0.0 76-77 0.09943603767320765 78-79 0.034128239179437 80-81 5.46051826870992E-5 82-83 0.0 84-85 0.0 86-87 0.1491267539184679 88-89 0.3260475458246693 90-91 0.2786502472522672 92-93 0.0 94-95 5.46051826870992E-5 96-97 5.46051826870992E-5 98-99 1.638155480612976E-4 100-101 7.644725576193888E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 915664.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.29941091881811 #Duplication Level Percentage of deduplicated Percentage of total 1 84.92166051160314 58.85021047701955 2 9.788486039192115 13.566726326061772 3 2.394762646796911 4.978669221402468 4 0.9161594048808213 2.539572282639035 5 0.49083038527299266 1.7007128280237462 6 0.3108542033347958 1.2925207903643912 7 0.2083876337064853 1.0108798181038114 8 0.1400210196249281 0.776269934100783 9 0.11843807287035588 0.7386919812248143 >10 0.6482914411868085 8.222203435630911 >50 0.04324330401383734 2.055132292711129 >100 0.01697880397356549 1.9210517794226223 >500 9.432667716553237E-4 0.4917839865169195 >1k 9.432667716553237E-4 1.8555748467780624 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4791 0.5232268605077846 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4387 0.4791058728966084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2798 0.3055706023170071 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2280 0.24899963305317235 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1757 0.1918826119624666 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1019 0.11128536231630817 RNA PCR Primer, Index 31 (95% over 22bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 963 0.10516958185535305 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0921036537419839E-4 0.0 10-11 0.0 0.0 0.0 1.0921036537419839E-4 0.0 12-13 0.0 0.0 0.0 2.1842073074839678E-4 0.0 14-15 0.0 0.0 0.0 3.276310961225952E-4 0.0 16-17 0.0 0.0 0.0 3.276310961225952E-4 0.0 18-19 0.0 0.0 0.0 3.276310961225952E-4 0.0 20-21 0.0 0.0 0.0 7.098673749322895E-4 0.0 22-23 0.0 0.0 0.0 0.0016927606633000752 0.0 24-25 0.0 0.0 0.0 0.0036039420573485467 0.0 26-27 0.0 0.0 0.0 0.008791434412622971 0.0 28-29 0.0 0.0 0.0 0.024190095930384944 0.0 30-31 0.0 0.0 0.0 0.07295252406996452 0.0 32-33 0.0 0.0 0.0 0.17025895961837528 0.0 34-35 0.0 0.0 0.0 0.32151531566164004 0.0 36-37 0.0 0.0 0.0 0.5435399884673854 0.0 38-39 0.0 0.0 0.0 0.845561253909731 0.0 40-41 0.0 0.0 0.0 1.1826390466371945 0.0 42-43 0.0 0.0 0.0 1.5093418546541089 0.0 44-45 0.0 0.0 0.0 1.9005333834244875 0.0 46-47 0.0 0.0 0.0 2.3574149469674466 0.0 48-49 0.0 0.0 0.0 2.8081807300494503 0.0 50-51 0.0 0.0 0.0 3.2866859459365005 0.0 52-53 0.0 0.0 0.0 3.760440510929773 0.0 54-55 0.0 0.0 0.0 4.288418022331335 0.0 56-57 0.0 0.0 0.0 4.899067780321166 0.0 58-59 0.0 0.0 0.0 5.53789381257754 0.0 60-61 0.0 0.0 0.0 6.204350067273586 0.0 62-63 0.0 0.0 0.0 6.8819457792378 0.0 64-65 0.0 0.0 0.0 7.594488808121756 0.0 66-67 0.0 0.0 0.0 8.366551486134652 0.0 68-69 0.0 0.0 0.0 9.217955494591902 0.0 70-71 0.0 0.0 0.0 10.141110713100002 0.0 72-73 0.0 0.0 0.0 11.041713991158328 0.0 74-75 0.0 0.0 0.0 11.96312184382044 0.0 76-77 0.0 0.0 0.0 12.947216446207342 0.0 78-79 0.0 0.0 0.0 14.013928689999826 0.0 80-81 1.0921036537419839E-4 0.0 0.0 15.127601390903212 0.0 82-83 1.0921036537419839E-4 0.0 0.0 16.254379335651507 0.0 84-85 1.0921036537419839E-4 0.0 0.0 17.371109926785373 0.0 86-87 1.0921036537419839E-4 0.0 0.0 18.5129589019553 0.0 88-89 1.0921036537419839E-4 0.0 0.0 19.691229533977527 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3810 0.0 40.87804 1 TAGCGTA 70 4.342741E-6 40.66842 7 ATAGCGT 90 5.746788E-7 36.90282 6 GTATCAA 5630 0.0 31.216578 1 GCGTAAG 85 6.305719E-4 28.01679 1 AATAGCG 90 9.005223E-4 26.359158 5 TATCAAC 6825 0.0 25.721905 2 TCAACGC 6940 0.0 25.364044 4 ATCAACG 6945 0.0 25.345785 3 CAACGCA 7075 0.0 24.880066 5 AACGCAG 7220 0.0 24.380398 6 TAGTACA 245 3.0195224E-10 23.239094 4 GAGTACT 4675 0.0 22.328978 12-13 GTACATG 3965 0.0 21.86228 1 ACGCAGA 8100 0.0 21.61451 7 CGCAGAG 8065 0.0 21.590652 8 GTCTTAG 310 1.0913936E-11 21.509663 1 TACATGG 4020 0.0 21.244694 2 ACATGGG 3920 0.0 21.060429 3 GAATAGG 230 8.761708E-8 20.708061 1 >>END_MODULE