FastQCFastQC Report
Thu 26 May 2016
SRR938788_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938788_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914774
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53410.5838600572381812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43390.4743248059083446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26850.29351511958144855No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18030.1970978624228498No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.101008555118532No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.10046197202806377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39000.036.3135451
GTATCAA57900.033.16041
GTACCGT700.00844163727.1091826
TCAACGC70750.026.8903394
TATCAAC70800.026.8713462
ATCAACG70700.026.7751433
CAACGCA71100.026.6912365
AACGCAG71950.026.2425966
ACGCAGA79050.023.5855057
CGCAGAG80200.022.9515428
GTACATG40100.021.688211
GAGTACT44250.021.61614412-13
GCAGAGT82300.021.2683329
TACATGG40900.020.8798542
TGAGACG1404.8896484E-420.3330024
AGTACTT45900.020.27028512-13
TAAGGCG1908.333185E-619.9762845
AGAGTAC68600.019.89327810-11
CGGTCCA600.004783689519.77795410-11
TACGCCG1200.004795559719.7681985
Produced by FastQC (version 0.11.5)