##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938788_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 914774 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.528717475573202 33.0 31.0 34.0 30.0 34.0 2 31.591290307770006 33.0 31.0 34.0 30.0 34.0 3 31.590764494836975 33.0 31.0 34.0 28.0 34.0 4 35.15264863233979 37.0 35.0 37.0 33.0 37.0 5 35.18873951380341 37.0 35.0 37.0 33.0 37.0 6 35.253697634607015 37.0 35.0 37.0 33.0 37.0 7 35.17438186918299 37.0 35.0 37.0 32.0 37.0 8 35.14015483605787 37.0 35.0 37.0 32.0 37.0 9 36.71563468135299 39.0 37.0 39.0 32.0 39.0 10-11 36.61104655357498 39.0 37.0 39.0 32.0 39.0 12-13 36.51122736326131 39.0 37.0 39.0 32.0 39.0 14-15 37.660127528766665 40.0 37.5 41.0 32.0 41.0 16-17 37.55837398089583 40.0 37.0 41.0 32.0 41.0 18-19 37.53744312802944 40.0 37.0 41.0 32.0 41.0 20-21 37.451670029974615 40.0 37.0 41.0 32.0 41.0 22-23 37.418573877263675 40.0 37.0 41.0 32.0 41.0 24-25 37.35097412038383 40.0 37.0 41.0 32.0 41.0 26-27 37.2089078832586 40.0 37.0 41.0 31.0 41.0 28-29 37.03236865061753 40.0 36.5 41.0 31.0 41.0 30-31 36.776067094167516 39.5 36.0 41.0 30.0 41.0 32-33 36.543591641214114 39.0 35.5 41.0 30.0 41.0 34-35 36.23682024193954 39.0 35.0 41.0 30.0 41.0 36-37 36.01043864386176 39.0 35.0 41.0 29.0 41.0 38-39 35.821761440530665 39.0 35.0 41.0 28.5 41.0 40-41 35.6669210099981 39.0 35.0 41.0 27.0 41.0 42-43 35.60238539792343 39.0 35.0 41.0 27.0 41.0 44-45 35.37467833585126 39.0 35.0 41.0 25.5 41.0 46-47 34.975606543255495 39.0 35.0 41.0 24.0 41.0 48-49 34.634930048296084 39.0 35.0 41.0 22.5 41.0 50-51 33.48820200399224 38.0 32.5 40.0 19.5 40.5 52-53 33.54460227334839 38.0 33.0 39.5 19.0 40.5 54-55 34.039765559580836 38.0 34.0 40.0 19.0 41.0 56-57 33.943267954707935 38.0 34.0 40.0 18.0 41.0 58-59 33.748858187924014 38.0 34.0 40.0 16.0 41.0 60-61 33.457386742517826 38.0 33.0 40.0 13.0 41.0 62-63 33.075490776956926 37.5 33.0 40.0 8.5 41.0 64-65 32.68993707735463 37.0 32.5 40.0 7.0 41.0 66-67 32.30040042677208 36.0 32.0 39.5 3.5 41.0 68-69 31.866784582858717 36.0 31.5 39.0 2.0 41.0 70-71 31.435933356216946 35.0 31.0 39.0 2.0 40.0 72-73 30.99235384914744 35.0 31.0 38.0 2.0 40.0 74-75 30.48693611755472 35.0 30.0 37.0 2.0 39.5 76-77 29.981781839011603 35.0 30.0 37.0 2.0 39.0 78-79 29.499061516833667 34.5 29.0 36.0 2.0 39.0 80-81 28.986334329572113 34.0 29.0 36.0 2.0 37.5 82-83 28.519299302341345 34.0 29.0 35.0 2.0 37.0 84-85 28.104644425836327 34.0 27.5 35.0 2.0 37.0 86-87 27.610059971096685 34.0 26.5 35.0 2.0 36.0 88-89 27.10080631937506 34.0 25.5 35.0 2.0 36.0 90-91 26.586224575687545 34.0 25.0 35.0 2.0 35.5 92-93 26.07197570110213 33.0 23.5 35.0 2.0 35.0 94-95 25.502374356944994 33.0 19.5 35.0 2.0 35.0 96-97 24.85537356767901 33.0 9.5 35.0 2.0 35.0 98-99 24.079556261983832 32.0 2.0 35.0 2.0 35.0 100-101 21.830696434310553 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.088474239820858 1101 2 0.12289244931254117 1101 3 0.15815923730143666 1101 4 -0.08044526632415483 1101 5 9.320651149451464E-4 1101 6 0.12458621717613738 1101 7 0.15805967966628742 1101 8 0.2444585692718988 1101 9 0.16129286426963318 1101 10-11 0.06307796682629885 1101 12-13 0.229641249314966 1101 14-15 0.05634496465246741 1101 16-17 0.26120519745183657 1101 18-19 0.24040141636401557 1101 20-21 0.2936383930612081 1101 22-23 0.29335925646319794 1101 24-25 0.41975723562124045 1101 26-27 0.32752814064956937 1101 28-29 0.46031562469507037 1101 30-31 0.3659569346284357 1101 32-33 0.15657758788625387 1101 34-35 0.24682349493888722 1101 36-37 0.19678365441182422 1101 38-39 0.2921256522599478 1101 40-41 0.46150761899760084 1101 42-43 0.6832676630126855 1101 44-45 0.5630740997603709 1101 46-47 0.44160741178781393 1101 48-49 0.18765057828287723 1101 50-51 0.2989998529731892 1101 52-53 0.33943051089791965 1101 54-55 0.3784843558025486 1101 56-57 0.24340402382546245 1101 58-59 0.08661161796119643 1101 60-61 0.15976890621449513 1101 62-63 0.2888824386270912 1101 64-65 0.05226902701969749 1101 66-67 0.04534129159967648 1101 68-69 0.07392888890173666 1101 70-71 -0.2304524251108404 1101 72-73 -0.19702925218912526 1101 74-75 -0.5057313376982009 1101 76-77 -0.4106746881527492 1101 78-79 -0.3473303384380664 1101 80-81 -0.3914942597958486 1101 82-83 -0.35751093403906964 1101 84-85 -0.514249924902682 1101 86-87 -0.6245710488302691 1101 88-89 -0.3747343129516558 1101 90-91 -0.31057319700388675 1101 92-93 -0.6933818161394143 1101 94-95 -0.8065998938716668 1101 96-97 -1.0377124555422093 1101 98-99 -1.0345629412914015 1101 100-101 -0.672334259944499 1102 1 0.17042198328879365 1102 2 0.10982831629591416 1102 3 0.03820674323968021 1102 4 0.3779870377138508 1102 5 0.29428123376102633 1102 6 0.3443011815466832 1102 7 0.2578221499750768 1102 8 0.34778398494885465 1102 9 0.5330268310154764 1102 10-11 0.5238855677764178 1102 12-13 0.44044885026509206 1102 14-15 0.5676276249850076 1102 16-17 0.42035009056347405 1102 18-19 0.5658170933236377 1102 20-21 0.4361562077880521 1102 22-23 0.4875397790285234 1102 24-25 0.39125367267586597 1102 26-27 0.383347618084251 1102 28-29 0.4864438907283244 1102 30-31 0.4621564595690515 1102 32-33 0.537218918052524 1102 34-35 0.5003864403070608 1102 36-37 0.6279000439605156 1102 38-39 0.849726602378702 1102 40-41 0.5256406356246828 1102 42-43 0.7034576867656526 1102 44-45 0.748941083133289 1102 46-47 0.7129850839968483 1102 48-49 0.8931137611807358 1102 50-51 0.706363273400747 1102 52-53 0.6024946439145467 1102 54-55 0.5542563272989867 1102 56-57 0.7861593682435171 1102 58-59 0.7772291951583483 1102 60-61 0.6864909964757828 1102 62-63 0.4539655740190156 1102 64-65 0.42697211490568776 1102 66-67 0.47764532960442807 1102 68-69 0.6932875587354594 1102 70-71 0.600307669901035 1102 72-73 0.28931279056622117 1102 74-75 0.15222590695737637 1102 76-77 0.16117804343869935 1102 78-79 0.20967678745267193 1102 80-81 0.12156987322077839 1102 82-83 0.3948643628730437 1102 84-85 0.4447762033633502 1102 86-87 0.23766173026711712 1102 88-89 0.34498065141889356 1102 90-91 0.08728903577521407 1102 92-93 0.15103623611711825 1102 94-95 -0.03522222166263944 1102 96-97 -0.37916138665859833 1102 98-99 -0.2952517773959116 1102 100-101 -0.301787941654716 1103 1 0.2564047385787944 1103 2 0.39830717397672544 1103 3 0.5198288016670851 1103 4 0.40016871935875997 1103 5 0.35302017693075527 1103 6 0.16676202179775856 1103 7 0.22402382438254875 1103 8 0.2728043232586117 1103 9 0.4445912347560892 1103 10-11 0.3115883046735064 1103 12-13 0.47432471991488967 1103 14-15 0.4409662095474829 1103 16-17 0.6602040628598402 1103 18-19 0.5400779079987856 1103 20-21 0.45358117370947326 1103 22-23 0.5819714355056291 1103 24-25 0.34089628980140674 1103 26-27 0.5690751581962914 1103 28-29 0.4800342622051659 1103 30-31 0.4984477585816549 1103 32-33 0.366443776772698 1103 34-35 0.6496192983089273 1103 36-37 0.6574735788539812 1103 38-39 0.7510592718260725 1103 40-41 0.8495305992469966 1103 42-43 0.7753491903857395 1103 44-45 0.7355626926214214 1103 46-47 0.7274456902553297 1103 48-49 0.9714584268298054 1103 50-51 0.9573679450900912 1103 52-53 1.0080861146124036 1103 54-55 1.024068561846633 1103 56-57 0.9753396595370845 1103 58-59 1.0020226150689382 1103 60-61 0.8604855765543107 1103 62-63 0.7074802244041578 1103 64-65 0.6199656692259765 1103 66-67 0.7370438053875574 1103 68-69 0.5662125749925089 1103 70-71 0.3898626214291987 1103 72-73 0.36592359830321186 1103 74-75 0.2376746965898775 1103 76-77 0.2936334964184333 1103 78-79 0.1524465523445322 1103 80-81 0.3035059170590202 1103 82-83 0.2829383589495862 1103 84-85 0.4153128626565312 1103 86-87 0.44690144479615057 1103 88-89 0.1265390420940129 1103 90-91 0.14143043260216714 1103 92-93 0.02048877507280622 1103 94-95 0.11295154776758665 1103 96-97 0.1482490771636975 1103 98-99 0.11306938016000956 1103 100-101 0.43809051823868117 1104 1 0.10214170912408704 1104 2 0.24838096146351774 1104 3 0.273668241144307 1104 4 0.5093322602144852 1104 5 0.4530592331848666 1104 6 0.3721769486262616 1104 7 0.4008726399819835 1104 8 0.43704242486253975 1104 9 0.35739618153211694 1104 10-11 0.44262269252570263 1104 12-13 0.5166227741794955 1104 14-15 0.5065656444113458 1104 16-17 0.6250101839447098 1104 18-19 0.5959879842502218 1104 20-21 0.6035100074477882 1104 22-23 0.454323670493217 1104 24-25 0.5362568524211682 1104 26-27 0.40449564202202737 1104 28-29 0.5016849262925476 1104 30-31 0.477485821391447 1104 32-33 0.6519574451510266 1104 34-35 0.6651081174732312 1104 36-37 0.456964274815455 1104 38-39 0.3561350121255771 1104 40-41 0.7573041044278455 1104 42-43 0.5310896733726764 1104 44-45 0.6283336158955422 1104 46-47 0.665907188390328 1104 48-49 0.6365490819001707 1104 50-51 0.8738013227221231 1104 52-53 0.8071194067925092 1104 54-55 0.6646193074541102 1104 56-57 0.8877848431670117 1104 58-59 0.9427023249517461 1104 60-61 0.7982209953343329 1104 62-63 0.8735302478641174 1104 64-65 0.9690466677030471 1104 66-67 0.6499549117804548 1104 68-69 0.6079562401467307 1104 70-71 0.6165107822466069 1104 72-73 0.6360205312077127 1104 74-75 0.3374365582887968 1104 76-77 0.5192010571849828 1104 78-79 0.6676090289828629 1104 80-81 0.4392440933678614 1104 82-83 0.45245036547119355 1104 84-85 0.39062813050641765 1104 86-87 0.22767135103493175 1104 88-89 0.44655720718401426 1104 90-91 0.31959117136824844 1104 92-93 0.49801439099517353 1104 94-95 0.4783188731481225 1104 96-97 0.06867720186061632 1104 98-99 0.3284973869017982 1104 100-101 0.4595151016604824 1105 1 0.15311207346505284 1105 2 0.2028421801653657 1105 3 0.23679644390975696 1105 4 0.24422646976813667 1105 5 0.22004124906495548 1105 6 0.29672350614933407 1105 7 0.20711460907161694 1105 8 0.06858297744036435 1105 9 0.3256001756141984 1105 10-11 0.12535245975088571 1105 12-13 0.27559819639420624 1105 14-15 0.3436642268756742 1105 16-17 0.4639529961839486 1105 18-19 0.3059319790490065 1105 20-21 0.5568181026054404 1105 22-23 0.4009344343447836 1105 24-25 0.3718222029144087 1105 26-27 0.32501202635899773 1105 28-29 0.33336344415124586 1105 30-31 0.43051107492505736 1105 32-33 0.32429374256219035 1105 34-35 0.16335731502210393 1105 36-37 0.45652529437632694 1105 38-39 0.4222674792724348 1105 40-41 0.40185153223283976 1105 42-43 0.508414989873728 1105 44-45 0.5257388959471427 1105 46-47 0.5417883488344799 1105 48-49 0.6158363722006257 1105 50-51 0.7288648277057703 1105 52-53 0.7356667014955605 1105 54-55 0.7619526612646652 1105 56-57 0.42111024157695454 1105 58-59 0.635954824452611 1105 60-61 0.5128299571903909 1105 62-63 0.5180866344535886 1105 64-65 0.7296218728592123 1105 66-67 0.5738116428991802 1105 68-69 0.5188560092660559 1105 70-71 0.4893037217005798 1105 72-73 0.4932827778660851 1105 74-75 0.21198030019389336 1105 76-77 0.42905520817377507 1105 78-79 0.3703348031498237 1105 80-81 0.42245277161439887 1105 82-83 0.48061550402519515 1105 84-85 0.6132283281116067 1105 86-87 0.6792849561009966 1105 88-89 0.5338247267269622 1105 90-91 0.45962081214658923 1105 92-93 0.7307853686289292 1105 94-95 0.9708395679402528 1105 96-97 0.9967253642859397 1105 98-99 0.4670577870267074 1105 100-101 0.9691048405247145 1106 1 0.13538186003014374 1106 2 0.22441905905768067 1106 3 0.16144485070581638 1106 4 0.2826169407760588 1106 5 0.4055337214030672 1106 6 0.3977864482861051 1106 7 0.4201589285588909 1106 8 0.41366188184812813 1106 9 0.1950336359201401 1106 10-11 0.49348112532921107 1106 12-13 0.3574187417717951 1106 14-15 0.5635209292155992 1106 16-17 0.4607538910385358 1106 18-19 0.42872779499918323 1106 20-21 0.34759807721091107 1106 22-23 0.4643113011444626 1106 24-25 0.3438658573070583 1106 26-27 0.29738883269553895 1106 28-29 0.31352548538711744 1106 30-31 0.2993362759650182 1106 32-33 0.310254804295063 1106 34-35 0.28360400466476676 1106 36-37 0.3285580654023832 1106 38-39 0.3483373746711891 1106 40-41 0.6127633165003274 1106 42-43 0.5352340236726292 1106 44-45 0.5573496342021755 1106 46-47 0.3233356007844179 1106 48-49 0.22231257995199627 1106 50-51 0.20770756305856963 1106 52-53 0.009669966505533978 1106 54-55 5.553579210584303E-4 1106 56-57 0.043240962902302726 1106 58-59 0.24041337696485954 1106 60-61 0.29853888759730296 1106 62-63 0.22168721489980925 1106 64-65 -0.019998418573166532 1106 66-67 -0.13378434234780912 1106 68-69 -0.005499755895080227 1106 70-71 -0.18559077010666414 1106 72-73 0.033886200080246454 1106 74-75 0.019812091716961078 1106 76-77 0.15049241719487938 1106 78-79 0.06823491802980541 1106 80-81 0.17056390287989132 1106 82-83 0.29156719397017383 1106 84-85 0.7122083148097111 1106 86-87 0.6322319299204544 1106 88-89 0.5732261415646214 1106 90-91 0.4366188168627154 1106 92-93 0.4665257398726581 1106 94-95 0.4472371735995999 1106 96-97 0.6532408730833268 1106 98-99 0.8960661914042412 1106 100-101 1.106419672134848 1107 1 0.060549639343733475 1107 2 0.20066468444367302 1107 3 0.1731049983398485 1107 4 0.08440266556504383 1107 5 -0.05941701823755352 1107 6 0.04652450081088233 1107 7 0.05430650377284252 1107 8 0.12920109897603282 1107 9 0.17300263865519128 1107 10-11 0.1887444844445909 1107 12-13 0.25403198178003095 1107 14-15 0.3205741304037275 1107 16-17 0.34145030017890576 1107 18-19 0.3897065321694342 1107 20-21 0.22315552802407979 1107 22-23 0.38180740442946615 1107 24-25 0.24217600641154746 1107 26-27 0.2532374342895096 1107 28-29 0.24003785194300065 1107 30-31 0.2974099753444861 1107 32-33 0.43803411788504576 1107 34-35 0.49859915215176187 1107 36-37 0.3421568655082581 1107 38-39 0.18410068967281035 1107 40-41 0.3153913199117895 1107 42-43 0.38883164741170617 1107 44-45 0.5755650857799779 1107 46-47 0.4781595869032884 1107 48-49 0.2563384012030667 1107 50-51 0.27269712280330083 1107 52-53 0.4801584528155729 1107 54-55 0.49980542409169004 1107 56-57 0.3914943523091239 1107 58-59 0.4313882909430333 1107 60-61 0.5413504042281971 1107 62-63 0.6502170505025475 1107 64-65 0.6487496330693432 1107 66-67 0.4075314239081109 1107 68-69 0.6486525247860406 1107 70-71 0.7235543053278519 1107 72-73 0.5394568850198098 1107 74-75 0.396353573350936 1107 76-77 0.3836424333699213 1107 78-79 0.2798591864767026 1107 80-81 0.4354075931425143 1107 82-83 0.31184011106572385 1107 84-85 0.567189934078602 1107 86-87 0.2065024354640812 1107 88-89 0.35614764849048086 1107 90-91 0.32290414462790906 1107 92-93 0.4426213784927775 1107 94-95 0.4238633536425418 1107 96-97 0.43975323963922364 1107 98-99 0.26385021046138135 1107 100-101 0.7235681590330607 1108 1 0.35262466985027174 1108 2 0.2654320307923008 1108 3 0.2632423182764789 1108 4 0.33373114776654944 1108 5 0.27627332698803286 1108 6 0.15187084195812872 1108 7 0.25724261017865047 1108 8 0.2590586187912578 1108 9 0.2922430102429203 1108 10-11 0.5584382473997422 1108 12-13 0.6163636660775467 1108 14-15 0.6700612840033813 1108 16-17 0.8698260559869908 1108 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-1.5242657040218077 2316 92-93 -1.1962138178669015 2316 94-95 -1.3600969787345498 2316 96-97 -1.483024483182028 2316 98-99 -1.7287618184237665 2316 100-101 -2.1036417529265776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6488.0 3 1245.0 4 1046.0 5 1119.0 6 1254.0 7 1539.0 8 1898.0 9 2259.0 10 2697.0 11 3435.0 12 4726.0 13 6334.0 14 6642.0 15 7714.0 16 9179.0 17 11731.0 18 10137.0 19 6873.0 20 6963.0 21 7160.0 22 7940.0 23 8829.0 24 9793.0 25 11441.0 26 13017.0 27 14971.0 28 17297.0 29 20655.0 30 23892.0 31 28122.0 32 34146.0 33 42351.0 34 54364.0 35 74214.0 36 105559.0 37 159888.0 38 159417.0 39 28434.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.573858024657525 20.485236068344776 14.92660511237513 22.014300794622567 2 15.48188945339874 22.04811229852838 40.14869481429572 22.321303433777153 3 18.15631811261651 26.605404102912345 32.08924327225331 23.149034512217828 4 12.761750007664716 18.452910182683 38.42025849797871 30.36508131167358 5 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19.99355709286826 26-27 20.359465025654526 27.789213306897242 31.682613333772107 20.168708333676125 28-29 20.209322689546532 28.054427408463816 31.502308109444797 20.233941792544854 30-31 20.555990431272463 27.661220122393757 31.473581848360666 20.309207597973113 32-33 20.17605348415913 27.97721403082962 31.37857084964371 20.46816163536754 34-35 20.70355590296348 27.91195951783437 31.17675292439764 20.207731654804515 36-37 20.703120284206957 27.703044557144846 31.015664526919874 20.57817063172832 38-39 20.49897347944929 27.940726256431525 31.058638409096144 20.501661855023038 40-41 20.69931542686278 27.578940902504545 31.233855053896015 20.487888616736665 42-43 20.382046796041827 27.879189866362076 30.87096991871123 20.867793418884865 44-45 20.447129736975082 27.96654016261358 30.59607605166896 20.990254048742376 46-47 20.75095170736686 27.89289958815131 30.129370748317985 21.226777956163847 48-49 20.61030328574188 28.099590818889062 29.921533375480745 21.36857251988831 50-51 20.47424188629982 28.39332740316048 29.84301870309205 21.289412007447652 52-53 20.540569313192496 28.450129808412548 29.69207611695324 21.317224761441718 54-55 20.36135309597965 28.221691062692187 29.696591580024013 21.72036426130415 56-57 20.602394501961037 28.39772015938476 29.303819100605182 21.69606623804902 58-59 20.59245867938582 28.104051049209566 29.435404693552265 21.868085577852348 60-61 20.421946342102682 27.879044095673777 29.904004904210524 21.795004658013017 62-63 20.41747365071353 27.771955909866513 29.994787751765305 21.815782687654654 64-65 20.311114184602687 27.79616384344512 29.76385705629758 22.12886491565461 66-67 20.261732430923793 28.04806093734306 29.288114792114108 22.402091839619043 68-69 20.35789738306338 27.606356789725684 29.62182117974842 22.413924647462515 70-71 20.364790023437457 27.475011938283195 29.509130728317608 22.651067309961743 72-73 20.29178475852182 27.36588021698898 29.363928545554735 22.978406478934463 74-75 20.08972060275473 27.588207239884067 29.353751520909103 22.9683206364521 76-77 20.157925783661433 27.20307291309418 29.48668632965224 23.15231497359215 78-79 20.044744422993894 27.14377958708512 29.42669973672329 23.384776253197696 80-81 20.138635514459672 27.143273340974343 29.212216911799 23.50587423276699 82-83 20.047594538864118 26.92882986790513 29.408640323778947 23.614935269451813 84-85 19.96619934703284 26.63315866004554 29.379461713627258 24.021180279294356 86-87 20.04296202003797 26.64879783159766 29.201279532959497 24.106960615404873 88-89 20.210471789119953 26.21086029164028 29.311417693985124 24.26725022525464 90-91 20.05980202106243 26.110530556330037 29.36918088355071 24.460486539056824 92-93 20.327218342229113 25.974945459035965 29.25574566759512 24.442090531139804 94-95 20.160208488393284 25.63818832895323 29.25802853761727 24.943574645036215 96-97 20.355018867924528 25.36381475128645 29.17274785591767 25.108418524871357 98-99 20.395078804444775 25.20633509247138 29.059039527091002 25.339546575992845 100-101 20.65863104742903 24.640553647800193 29.43141573739043 25.269399567380347 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1830.0 1 1206.0 2 626.5 3 673.0 4 615.0 5 631.5 6 834.0 7 1300.0 8 1904.0 9 2147.0 10 1879.5 11 1620.0 12 1669.0 13 1754.0 14 1880.5 15 1994.0 16 2024.0 17 2116.0 18 2310.0 19 2523.5 20 2813.5 21 3103.5 22 3470.5 23 4072.5 24 4700.5 25 5607.5 26 6923.0 27 8463.5 28 9954.0 29 11560.5 30 13428.5 31 15412.0 32 17662.0 33 19989.5 34 22591.5 35 25301.5 36 27913.0 37 29989.5 38 30583.0 39 29799.5 40 29252.0 41 29642.5 42 29974.0 43 30614.0 44 31609.0 45 32170.5 46 32248.5 47 32731.5 48 33889.0 49 33592.0 50 31730.0 51 29611.5 52 27482.0 53 26047.0 54 24608.5 55 22283.5 56 19637.0 57 16899.0 58 14705.0 59 12883.0 60 10818.5 61 8861.5 62 7082.5 63 5482.0 64 4058.0 65 3007.5 66 2313.5 67 1777.5 68 1314.0 69 973.5 70 763.0 71 595.0 72 464.0 73 383.5 74 296.5 75 233.5 76 186.0 77 118.0 78 91.0 79 77.5 80 55.5 81 55.5 82 52.0 83 33.0 84 19.5 85 19.0 86 14.5 87 8.0 88 10.0 89 9.0 90 4.0 91 5.0 92 8.5 93 7.0 94 3.0 95 1.0 96 0.0 97 0.0 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2751499277417154 2 0.16331902743191215 3 0.19283451431719748 4 0.16375629390428675 5 0.23546799537372073 6 0.19589537962381967 7 0.23984066009746668 8 0.24672760703736663 9 0.15205941576826626 10-11 0.20535126708892032 12-13 0.21240218895596072 14-15 0.17468795571365167 16-17 0.28515239829728434 18-19 0.23087669741378744 20-21 0.1986829533852077 22-23 0.24552512423833645 24-25 0.23421085426564375 26-27 0.3434181557411995 28-29 0.314886518418757 30-31 0.2881586052948597 32-33 0.19195998137244827 34-35 0.3565361499124374 36-37 0.39430504146379325 38-39 0.3763771160964347 40-41 0.34008399888934315 42-43 0.3709659435007991 44-45 0.3796019563301974 46-47 0.3844665458353648 48-49 0.40294105429319155 50-51 0.3951249160994956 52-53 0.39610876566233844 54-55 0.3891671604133917 56-57 0.37107526011889275 58-59 0.34795479539208596 60-61 0.3175101172530046 62-63 0.3781261819859331 64-65 0.4108118507959343 66-67 0.4160043901553827 68-69 0.4200491050248477 70-71 0.41966649686151986 72-73 0.41103048403212156 74-75 0.4070404274717033 76-77 0.39206405079287343 78-79 0.3689982443751134 80-81 0.3786181067673546 82-83 0.3864342449610505 84-85 0.3879646776143616 86-87 0.38304542980014733 88-89 0.3916814426295457 90-91 0.41190501697687076 92-93 0.41119445895926204 94-95 0.419338547007239 96-97 0.41939320531628577 98-99 0.4172068729544127 100-101 0.39146280939335837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 914774.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.13585119443636 #Duplication Level Percentage of deduplicated Percentage of total 1 85.90985103512475 61.11270379370828 2 9.24514701363567 13.153228044653492 3 2.166703897197902 4.623909780404258 4 0.854518378241664 2.4314756879004036 5 0.45107046490852176 1.6043640734968914 6 0.2715584678252561 1.159052565468393 7 0.18102253979954194 0.9014034717813403 8 0.1452480378076081 0.8265874243012697 9 0.1121453545664257 0.7179799729126105 >10 0.6091291908238285 7.963786778182716 >50 0.03820459362715622 1.8689993654720418 >100 0.014014934219291444 1.653645946782878 >500 7.70051234642962E-4 0.43066660181175764 >1k 4.620307407857772E-4 0.967055226200047 >5k 1.540102469285924E-4 0.5851412669235811 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5341 0.5838600572381812 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4339 0.4743248059083446 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2685 0.29351511958144855 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1803 0.1970978624228498 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 924 0.101008555118532 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 919 0.10046197202806377 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.0931661809364936E-4 5 0.0 0.0 0.0 0.0 1.0931661809364936E-4 6 0.0 0.0 0.0 0.0 1.0931661809364936E-4 7 0.0 0.0 0.0 0.0 1.0931661809364936E-4 8 0.0 0.0 0.0 0.0 1.0931661809364936E-4 9 0.0 0.0 0.0 1.0931661809364936E-4 1.0931661809364936E-4 10-11 0.0 0.0 0.0 2.1863323618729873E-4 1.0931661809364936E-4 12-13 0.0 0.0 0.0 2.1863323618729873E-4 1.0931661809364936E-4 14-15 0.0 0.0 0.0 2.1863323618729873E-4 1.0931661809364936E-4 16-17 0.0 0.0 0.0 3.8260816332777274E-4 1.0931661809364936E-4 18-19 0.0 0.0 0.0 5.465830904682468E-4 1.0931661809364936E-4 20-21 0.0 0.0 0.0 0.0011478244899833182 1.0931661809364936E-4 22-23 0.0 0.0 0.0 0.0020223574347325132 1.0931661809364936E-4 24-25 0.0 0.0 0.0 0.004263348105652326 1.0931661809364936E-4 26-27 0.0 0.0 0.0 0.009291912537960197 2.1863323618729873E-4 28-29 0.0 0.0 0.0 0.026454621578663146 2.1863323618729873E-4 30-31 0.0 0.0 0.0 0.06881481108995227 2.1863323618729873E-4 32-33 0.0 0.0 0.0 0.15807182976341697 2.1863323618729873E-4 34-35 0.0 0.0 0.0 0.30324429859178337 2.1863323618729873E-4 36-37 0.0 0.0 0.0 0.5130775470225433 2.1863323618729873E-4 38-39 0.0 0.0 0.0 0.8003616193726537 2.1863323618729873E-4 40-41 0.0 0.0 0.0 1.1156854042637854 2.1863323618729873E-4 42-43 0.0 0.0 0.0 1.4181098282198663 2.1863323618729873E-4 44-45 0.0 0.0 0.0 1.7938310446077392 2.1863323618729873E-4 46-47 0.0 0.0 0.0 2.223500012024828 2.1863323618729873E-4 48-49 0.0 0.0 0.0 2.6613677257989403 2.1863323618729873E-4 50-51 0.0 0.0 0.0 3.130226700802603 2.1863323618729873E-4 52-53 0.0 0.0 0.0 3.581157750438906 2.1863323618729873E-4 54-55 0.0 0.0 0.0 4.083412952270178 2.1863323618729873E-4 56-57 0.0 0.0 0.0 4.663118978020801 2.1863323618729873E-4 58-59 0.0 0.0 0.0 5.279063462669468 2.1863323618729873E-4 60-61 0.0 0.0 0.0 5.928458832454792 3.279498542809481E-4 62-63 0.0 0.0 0.0 6.571349863463544 3.279498542809481E-4 64-65 0.0 0.0 0.0 7.266986162702482 3.279498542809481E-4 66-67 0.0 0.0 0.0 8.023183868365301 3.279498542809481E-4 68-69 0.0 0.0 0.0 8.866561576957807 3.279498542809481E-4 70-71 0.0 0.0 0.0 9.778371488476935 3.279498542809481E-4 72-73 0.0 0.0 0.0 10.660228646638405 3.279498542809481E-4 74-75 0.0 0.0 0.0 11.575700664863671 3.279498542809481E-4 76-77 0.0 0.0 0.0 12.521726677846113 3.279498542809481E-4 78-79 0.0 0.0 0.0 13.576686700758877 3.279498542809481E-4 80-81 0.0 0.0 0.0 14.657226812305552 3.279498542809481E-4 82-83 0.0 0.0 0.0 15.751814109277266 3.279498542809481E-4 84-85 0.0 0.0 0.0 16.86400138176205 3.279498542809481E-4 86-87 0.0 0.0 0.0 17.99242217203375 3.279498542809481E-4 88-89 0.0 0.0 0.0 19.154020555896864 3.279498542809481E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3900 0.0 36.313545 1 GTATCAA 5790 0.0 33.1604 1 GTACCGT 70 0.008441637 27.109182 6 TCAACGC 7075 0.0 26.890339 4 TATCAAC 7080 0.0 26.871346 2 ATCAACG 7070 0.0 26.775143 3 CAACGCA 7110 0.0 26.691236 5 AACGCAG 7195 0.0 26.242596 6 ACGCAGA 7905 0.0 23.585505 7 CGCAGAG 8020 0.0 22.951542 8 GTACATG 4010 0.0 21.68821 1 GAGTACT 4425 0.0 21.616144 12-13 GCAGAGT 8230 0.0 21.268332 9 TACATGG 4090 0.0 20.879854 2 TGAGACG 140 4.8896484E-4 20.333002 4 AGTACTT 4590 0.0 20.270285 12-13 TAAGGCG 190 8.333185E-6 19.976284 5 AGAGTAC 6860 0.0 19.893278 10-11 CGGTCCA 60 0.0047836895 19.777954 10-11 TACGCCG 120 0.0047955597 19.768198 5 >>END_MODULE