FastQCFastQC Report
Thu 26 May 2016
SRR938788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914774
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48870.5342303126236644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44070.4817583359387127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27780.30368156506415794No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT21460.23459346242897156No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17770.19425563035241492No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT9960.10887935162127477RNA PCR Primer, Index 31 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.10396010380706056No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCC405.4131704E-447.45443
GGTATCA40200.036.6784171
GTATCAA58100.030.4538171
TATCAAC70350.024.823342
ATCAACG72450.024.1693213
TCAACGC72550.024.136014
CAACGCA72750.024.0696545
AACGCAG74150.023.610046
GAGTACT45950.021.01166212-13
ACCGAGC1150.003749410220.6267098
ACGCAGA84600.020.5243077
CGCAGAG84150.020.464938
TACATGG42250.019.7679862
GTACATG42600.019.6506981
GTCCTAC6550.019.606381
GTACTTT49800.019.33963214-15
GCAGAGT87550.019.12839
ACATGGG41900.018.800553
AGTACTT47650.018.17111212-13
AGAGTAC72050.018.04255910-11
Produced by FastQC (version 0.11.5)