FastQCFastQC Report
Thu 26 May 2016
SRR938787_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938787_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758225
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162910.590633468988208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124640.45188481722847124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84990.30813294781970285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27980.1014420505941321No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83550.047.719251
GTATCAA144050.041.4010541
ATCAACG185350.031.8255863
TATCAAC186400.031.6971472
TCAACGC186900.031.5362974
CAACGCA189250.031.0946275
AACGCAG195050.030.2689296
GTACATG140700.026.9550381
ACGCAGA218050.026.7292277
CGCAGAG219900.026.4397648
TACATGG143450.025.9610122
ACATGGG144300.024.7573783
GCAGAGT231050.024.3241169
GAGTACT131400.022.32267212-13
CATGGGA86700.021.3676764
AGTACTT135850.021.33005512-13
AGAGTAC211250.020.83267410-11
CATGGGG55900.020.681294
CAGAGTA227200.020.41213610-11
GTACTTT149750.019.74682814-15
Produced by FastQC (version 0.11.5)