##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938787_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2758225 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.591436521676076 33.0 31.0 34.0 30.0 34.0 2 31.640193602769898 33.0 31.0 34.0 30.0 34.0 3 31.64339711227329 33.0 31.0 34.0 28.0 34.0 4 35.22171722756483 37.0 35.0 37.0 33.0 37.0 5 35.264065476891844 37.0 35.0 37.0 33.0 37.0 6 35.35543111965122 37.0 35.0 37.0 33.0 37.0 7 35.27175012915915 37.0 35.0 37.0 32.0 37.0 8 35.24251647345666 37.0 35.0 37.0 32.0 37.0 9 36.804598247061065 39.0 37.0 39.0 32.0 39.0 10-11 36.69763942390487 39.0 37.0 39.0 32.0 39.0 12-13 36.579563124835715 39.0 37.0 39.0 32.0 39.0 14-15 37.71195152679712 40.0 37.0 41.0 32.0 41.0 16-17 37.656650744591175 40.0 37.0 41.0 32.0 41.0 18-19 37.624400112391115 40.0 37.0 41.0 32.0 41.0 20-21 37.53236592373719 40.0 37.0 41.0 32.0 41.0 22-23 37.42856909787997 40.0 37.0 41.0 32.0 41.0 24-25 37.420614163094015 40.0 37.0 41.0 32.0 41.0 26-27 37.320879188608615 40.0 37.0 41.0 31.0 41.0 28-29 37.19837703595609 40.0 36.5 41.0 31.0 41.0 30-31 36.96031995214314 39.5 36.0 41.0 30.5 41.0 32-33 36.711261409058366 39.0 35.5 41.0 30.0 41.0 34-35 36.3625409457169 39.0 35.0 41.0 30.0 41.0 36-37 36.09218646049543 39.0 35.0 41.0 29.0 41.0 38-39 35.907810820364546 39.0 35.0 41.0 29.0 41.0 40-41 35.7034491384858 39.0 35.0 40.5 27.5 41.0 42-43 35.410941819467226 38.5 35.0 40.0 26.5 41.0 44-45 35.29473538235641 39.0 35.0 41.0 25.5 41.0 46-47 35.02457921308088 39.0 35.0 41.0 24.0 41.0 48-49 34.733881391112035 39.0 34.5 41.0 23.0 41.0 50-51 33.56577074024055 38.0 32.5 40.0 21.5 40.5 52-53 33.6550301371353 38.0 33.0 39.5 21.5 40.5 54-55 34.17465725239964 38.0 34.0 40.0 22.0 41.0 56-57 34.086055887391346 38.0 34.0 40.0 20.5 41.0 58-59 33.8702556898005 38.0 34.0 40.0 20.0 41.0 60-61 33.59642269938094 38.0 33.0 40.0 18.5 41.0 62-63 33.238980141214 37.0 33.0 40.0 16.5 41.0 64-65 32.84672606476991 36.5 32.5 40.0 13.0 41.0 66-67 32.46066038847447 36.0 32.0 39.0 9.5 41.0 68-69 32.05874303220368 35.5 32.0 39.0 7.5 41.0 70-71 31.658335342475688 35.0 31.0 38.5 6.5 40.0 72-73 31.242319970270735 35.0 31.0 38.0 2.0 40.0 74-75 30.783028940713685 35.0 31.0 37.0 2.0 39.0 76-77 30.342084130192426 35.0 30.0 36.5 2.0 39.0 78-79 29.917152697840095 35.0 30.0 36.0 2.0 39.0 80-81 29.476384268868564 34.0 29.5 36.0 2.0 37.5 82-83 29.006532462000017 34.0 29.0 35.0 2.0 37.0 84-85 28.588591576104196 34.0 29.0 35.0 2.0 36.5 86-87 28.19494820038249 34.0 28.5 35.0 2.0 36.0 88-89 27.81554296694432 34.0 27.0 35.0 2.0 36.0 90-91 27.388230836860664 34.0 26.5 35.0 2.0 35.5 92-93 26.929392453480045 34.0 25.5 35.0 2.0 35.0 94-95 26.41696834014629 33.0 25.0 35.0 2.0 35.0 96-97 25.83703613737095 33.0 23.0 35.0 2.0 35.0 98-99 25.1442652430458 33.0 17.0 35.0 2.0 35.0 100-101 23.020186170453826 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33199897380714205 1101 2 0.12584944280395405 1101 3 0.29934533256084705 1101 4 0.33228633734226776 1101 5 0.18361603975125007 1101 6 0.15206364818367035 1101 7 0.26792735880974305 1101 8 0.27151391692142823 1101 9 0.3793694031989361 1101 10-11 0.35886483817922965 1101 12-13 0.3468560854530125 1101 14-15 0.4685869675369929 1101 16-17 0.5993564587973523 1101 18-19 0.5427775734250488 1101 20-21 0.5231286257219878 1101 22-23 0.47419262156464725 1101 24-25 0.4716507825550025 1101 26-27 0.4327840105804839 1101 28-29 0.3918695609917364 1101 30-31 0.4029342459703642 1101 32-33 0.2965867604999559 1101 34-35 0.30425215718342 1101 36-37 0.49852754278997224 1101 38-39 0.5165105496388236 1101 40-41 0.511019870805498 1101 42-43 0.5587963338392825 1101 44-45 0.6349252910492851 1101 46-47 0.4019975884889888 1101 48-49 0.3304655762537223 1101 50-51 0.33209255949451233 1101 52-53 0.24776182367233446 1101 54-55 0.27499752981405123 1101 56-57 0.38101079351128675 1101 58-59 0.25106869346524974 1101 60-61 0.17501199135221412 1101 62-63 0.2202165993156413 1101 64-65 0.17226576030806484 1101 66-67 -0.022609647190868998 1101 68-69 -0.056278099787803626 1101 70-71 -0.12844666256841109 1101 72-73 -0.13714441419010726 1101 74-75 -0.27620183582584446 1101 76-77 -0.4020140772221481 1101 78-79 -0.4971148556017049 1101 80-81 -0.43556526731843803 1101 82-83 -0.5270277275563053 1101 84-85 -0.48392626917768666 1101 86-87 -0.666228266355823 1101 88-89 -0.7340771315182408 1101 90-91 -0.7944788907620897 1101 92-93 -0.9346017531183399 1101 94-95 -1.0061531120646627 1101 96-97 -1.1084247027254612 1101 98-99 -1.162756761567156 1101 100-101 -0.9294032317681875 1102 1 0.3143361621983374 1102 2 0.31678099120728476 1102 3 0.3591216408607387 1102 4 0.37733645553024786 1102 5 0.3562440176095194 1102 6 0.3581876838292928 1102 7 0.43220202624002724 1102 8 0.3708437308760253 1102 9 0.3937451473447666 1102 10-11 0.3357593053803356 1102 12-13 0.4619654825961277 1102 14-15 0.37491475508574723 1102 16-17 0.40576642303268784 1102 18-19 0.5640528557342464 1102 20-21 0.5084087533805288 1102 22-23 0.5536206974016409 1102 24-25 0.5533836267254486 1102 26-27 0.46128971476602487 1102 28-29 0.5184611634112386 1102 30-31 0.583478466329332 1102 32-33 0.5511997605229411 1102 34-35 0.3897557207865461 1102 36-37 0.45325858616747894 1102 38-39 0.4692601727419543 1102 40-41 0.5760061323188808 1102 42-43 0.4990838121856953 1102 44-45 0.6427606965352055 1102 46-47 0.40523822414498767 1102 48-49 0.36832196130462336 1102 50-51 0.4488264609400332 1102 52-53 0.4073729108571129 1102 54-55 0.32296607736471117 1102 56-57 0.29839909418783606 1102 58-59 0.2169743504273569 1102 60-61 0.3090198951114118 1102 62-63 0.2543029499933098 1102 64-65 0.24921886859785047 1102 66-67 0.1877357247682525 1102 68-69 0.11108588127837038 1102 70-71 -0.09756250809999045 1102 72-73 -0.017512026223588606 1102 74-75 -0.04007440380771854 1102 76-77 0.0790631346102586 1102 78-79 -0.09581791627985581 1102 80-81 -0.2499415595118215 1102 82-83 -0.33253676250088304 1102 84-85 -0.34264212067689215 1102 86-87 -0.34049155723221247 1102 88-89 -0.31217246225961404 1102 90-91 -0.20672406958010825 1102 92-93 -0.33936996477673986 1102 94-95 -0.39277935108982476 1102 96-97 -0.4322198772580599 1102 98-99 -0.5905456347707236 1102 100-101 -0.5633048751953531 1103 1 0.17948674850403634 1103 2 0.32349479210718357 1103 3 0.33121777985676104 1103 4 0.36290971210365086 1103 5 0.24409631638322793 1103 6 0.3576090523257065 1103 7 0.21928642655608854 1103 8 0.28996008218253877 1103 9 0.3359736984113866 1103 10-11 0.4170425957650963 1103 12-13 0.5183391953729029 1103 14-15 0.5624684782084799 1103 16-17 0.6041109954003616 1103 18-19 0.7279671333020445 1103 20-21 0.54937752433311 1103 22-23 0.6527623541127952 1103 24-25 0.5917032287801547 1103 26-27 0.6005084844470545 1103 28-29 0.5696795507287362 1103 30-31 0.4149786687306829 1103 32-33 0.43676184238763227 1103 34-35 0.5074948154227243 1103 36-37 0.5112200949057453 1103 38-39 0.4307303156256381 1103 40-41 0.5588243070925216 1103 42-43 0.4869745118660731 1103 44-45 0.5277223061001592 1103 46-47 0.573613409529969 1103 48-49 0.5932149937615421 1103 50-51 0.7234702839290534 1103 52-53 0.6096450118728924 1103 54-55 0.6785410452991272 1103 56-57 0.6799621412362029 1103 58-59 0.5457081018473886 1103 60-61 0.6123223581249206 1103 62-63 0.5695023639419148 1103 64-65 0.408227419119946 1103 66-67 0.3820563548363296 1103 68-69 0.2767481705109489 1103 70-71 0.4101930688575486 1103 72-73 0.33126028168137367 1103 74-75 0.38181415786814554 1103 76-77 0.424579144362621 1103 78-79 0.5771485485240007 1103 80-81 0.5019258285593331 1103 82-83 0.6114944770181516 1103 84-85 0.4665434450101955 1103 86-87 0.4468287536012099 1103 88-89 0.3006482645960986 1103 90-91 0.2618459775930191 1103 92-93 0.21229705642887708 1103 94-95 -0.0033872761872615342 1103 96-97 -0.0582802404316034 1103 98-99 0.12881531849326677 1103 100-101 0.25612925969520006 1104 1 0.22754647099906222 1104 2 0.23514437244906716 1104 3 0.32216618081715254 1104 4 0.32923944054982 1104 5 0.24711205026457606 1104 6 0.29736121511142954 1104 7 0.350317381289301 1104 8 0.2671522571637013 1104 9 0.2382198880681301 1104 10-11 0.26671411031607306 1104 12-13 0.21958952151307187 1104 14-15 0.38910086473368466 1104 16-17 0.46540204142350206 1104 18-19 0.4262707646713011 1104 20-21 0.37330404678474594 1104 22-23 0.3838419733569651 1104 24-25 0.4241788745376951 1104 26-27 0.43078367346176805 1104 28-29 0.4302841633035257 1104 30-31 0.43580033675076635 1104 32-33 0.24012497051894144 1104 34-35 0.2917352826020263 1104 36-37 0.5543693923485122 1104 38-39 0.3899230710630235 1104 40-41 0.37132727533452936 1104 42-43 0.5104501546920162 1104 44-45 0.6577191399753559 1104 46-47 0.5749666347264579 1104 48-49 0.5612372797622882 1104 50-51 0.4981461225856876 1104 52-53 0.504873984260179 1104 54-55 0.5022695383702995 1104 56-57 0.4493619846889203 1104 58-59 0.5724870682248948 1104 60-61 0.5597608752058747 1104 62-63 0.5629865963472085 1104 64-65 0.37418674027933463 1104 66-67 0.33311905876553993 1104 68-69 0.4246976649570371 1104 70-71 0.44661669635710766 1104 72-73 0.3177130785715363 1104 74-75 0.25729941046920857 1104 76-77 0.2712289152583125 1104 78-79 0.3984177248331804 1104 80-81 0.3694664531654013 1104 82-83 0.40836690270949916 1104 84-85 0.35110536180560814 1104 86-87 0.336960265044695 1104 88-89 0.3750634842753229 1104 90-91 0.35278045346744236 1104 92-93 0.2303411828276154 1104 94-95 0.1819891848474171 1104 96-97 0.2626910856176856 1104 98-99 0.3428737921279499 1104 100-101 0.39158091619411195 1105 1 0.16903499374233633 1105 2 0.23122802263918274 1105 3 0.18550373890756333 1105 4 0.33310931803185895 1105 5 0.30033835641864215 1105 6 0.1991327965788301 1105 7 0.19849228623226622 1105 8 0.2381661988620536 1105 9 0.19525551685280362 1105 10-11 0.27600787175812513 1105 12-13 0.2246824661687583 1105 14-15 0.31755671602223856 1105 16-17 0.2193550057947249 1105 18-19 0.22977872129713006 1105 20-21 0.29234291454984884 1105 22-23 0.35256046371043936 1105 24-25 0.34922957162260815 1105 26-27 0.33339034857795724 1105 28-29 0.35051652494273355 1105 30-31 0.15389497130927765 1105 32-33 0.16375333272144843 1105 34-35 0.25950672324418633 1105 36-37 0.26092339882647764 1105 38-39 0.3184232823008699 1105 40-41 0.2964948573652322 1105 42-43 0.2849636035020424 1105 44-45 0.3876761882784052 1105 46-47 0.5176592132365272 1105 48-49 0.31299125807520056 1105 50-51 0.21136257097323607 1105 52-53 0.25961345757379206 1105 54-55 0.35000225207259916 1105 56-57 0.17333959347642036 1105 58-59 0.2453937010935121 1105 60-61 0.23333994857582496 1105 62-63 0.21126712021708016 1105 64-65 0.14895930752338415 1105 66-67 0.10469278077653144 1105 68-69 0.22657697772988428 1105 70-71 0.3135488185934072 1105 72-73 0.31804331374967987 1105 74-75 0.2760608525196204 1105 76-77 0.1270485181311507 1105 78-79 0.09375781777783487 1105 80-81 0.16466220024328138 1105 82-83 0.26393372428392325 1105 84-85 0.13815014423062166 1105 86-87 0.14347343220425302 1105 88-89 0.10894533190241518 1105 90-91 0.2240035206410802 1105 92-93 0.20274295722558477 1105 94-95 0.3071708494017038 1105 96-97 0.2015694126138854 1105 98-99 0.29530936458253265 1105 100-101 0.47606972058785857 1106 1 0.25215134241424053 1106 2 0.26490927965377153 1106 3 0.14170276621770128 1106 4 0.19182985675239905 1106 5 0.10835182443210556 1106 6 0.048201258980625994 1106 7 0.12974181859290468 1106 8 0.330792461488322 1106 9 0.2479191906275915 1106 10-11 0.3479177108901865 1106 12-13 0.2284628627269285 1106 14-15 0.33835918515804053 1106 16-17 0.3691267977887378 1106 18-19 0.34501254097538947 1106 20-21 0.3947054285790301 1106 22-23 0.2979738569016135 1106 24-25 0.2359280377655253 1106 26-27 0.10595675336657706 1106 28-29 0.26747873968272984 1106 30-31 0.1795702425401089 1106 32-33 0.19283863014008062 1106 34-35 0.3013192933900939 1106 36-37 0.2778070501545713 1106 38-39 0.37358976191180204 1106 40-41 0.33128909604835854 1106 42-43 0.5316863868914936 1106 44-45 0.4141165174965096 1106 46-47 0.39432213878833977 1106 48-49 0.6113021447194242 1106 50-51 0.5609697539137457 1106 52-53 0.4306142057967648 1106 54-55 0.44295025057615334 1106 56-57 0.5408311759680018 1106 58-59 0.5140319352872993 1106 60-61 0.4991255826947878 1106 62-63 0.39698728482613177 1106 64-65 0.2617614025477053 1106 66-67 0.3057459046074342 1106 68-69 0.5106454401316753 1106 70-71 0.4212162334830474 1106 72-73 0.420125244164943 1106 74-75 0.32614502760379693 1106 76-77 0.10799689074618968 1106 78-79 0.40384760453428825 1106 80-81 0.23529257809587634 1106 82-83 0.2841956023235781 1106 84-85 0.36171355612736633 1106 86-87 0.46498484260455086 1106 88-89 0.5060328739899624 1106 90-91 0.5701445606710074 1106 92-93 0.6527148988641933 1106 94-95 0.6208090835954962 1106 96-97 0.8252226112670833 1106 98-99 0.9164335695956254 1106 100-101 0.9812642585601736 1107 1 0.14233796884859018 1107 2 0.08294022664646761 1107 3 -0.012383328487700851 1107 4 0.10993991912475565 1107 5 0.07215377839799686 1107 6 0.05416619062861372 1107 7 -0.10899405256554928 1107 8 -0.012343819352878427 1107 9 -0.07770744611016056 1107 10-11 -0.08641775058552525 1107 12-13 0.040465708427404934 1107 14-15 -0.06119556084478717 1107 16-17 0.019695017938019532 1107 18-19 0.138691896767682 1107 20-21 0.03568535535130479 1107 22-23 0.1231190550887078 1107 24-25 0.03663394320731328 1107 26-27 0.12666903710253763 1107 28-29 0.07241087831249615 1107 30-31 0.12163631921456641 1107 32-33 0.11242060655265362 1107 34-35 0.1120477068507455 1107 36-37 0.1884704540784483 1107 38-39 0.23368739887646228 1107 40-41 0.3643121008382053 1107 42-43 0.2754979082986395 1107 44-45 0.24998644935915593 1107 46-47 0.18267439235006577 1107 48-49 0.24208354708552093 1107 50-51 0.45422838760953965 1107 52-53 0.2955667059040863 1107 54-55 0.29441633828996316 1107 56-57 0.30569842772049327 1107 58-59 0.26308040419004897 1107 60-61 0.0685888860378725 1107 62-63 0.008152366118714838 1107 64-65 -0.029498494540973752 1107 66-67 0.010148154182722635 1107 68-69 0.030271573965464427 1107 70-71 0.12350935289820697 1107 72-73 -0.09049645552779495 1107 74-75 -0.13758517055263297 1107 76-77 -0.06735038897267742 1107 78-79 -0.21059555198537083 1107 80-81 -0.18293646399473218 1107 82-83 -0.0046515823341728435 1107 84-85 -0.17823419092420778 1107 86-87 -0.1809952988218555 1107 88-89 -0.24546571849223753 1107 90-91 -0.13090540467768008 1107 92-93 -0.012967425288074708 1107 94-95 -0.1787763272831775 1107 96-97 0.05389181698546963 1107 98-99 0.29648422797050955 1107 100-101 0.3809968608428669 1108 1 0.0354637932314823 1108 2 0.06033721804148229 1108 3 0.06147117185775386 1108 4 -0.020980720282317122 1108 5 0.058162430492714634 1108 6 0.140077879592873 1108 7 0.07056125174950267 1108 8 0.13030700345975532 1108 9 -0.010346334999049134 1108 10-11 0.14771444902504527 1108 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Count 2 8935.0 3 2693.0 4 2732.0 5 3045.0 6 3522.0 7 4250.0 8 5093.0 9 6519.0 10 8464.0 11 11248.0 12 15356.0 13 20137.0 14 20953.0 15 21066.0 16 25430.0 17 33741.0 18 28206.0 19 19195.0 20 18881.0 21 20346.0 22 22499.0 23 25276.0 24 28299.0 25 32504.0 26 37259.0 27 43443.0 28 50520.0 29 59259.0 30 70276.0 31 84348.0 32 103100.0 33 128723.0 34 169878.0 35 234413.0 36 339229.0 37 497995.0 38 468867.0 39 82491.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02262920938664 19.4232957826062 14.310516503461054 22.243558504546105 2 15.440994226088673 21.5970266406984 40.090816356524414 22.871162776688507 3 18.2513426828547 26.771613828408743 31.208191408644044 23.768852080092515 4 12.516662547528862 17.55456768832172 38.2296635263231 31.699106237826314 5 12.952223583870087 38.01291694012557 34.32757424046185 14.707285235542495 6 29.41669728370918 36.88813820212616 18.786738184209295 14.908426329955363 7 26.520537948479305 34.2879951774839 22.245342605021932 16.946124269014863 8 25.794676188223892 34.19193230834408 21.301848549785777 18.711542953646244 9 27.092851681347106 16.60102663952562 21.367878196097433 34.93824348302984 10-11 24.180318429394553 27.013814469747103 28.151264390412695 20.654602710445648 12-13 25.41337966109134 25.140027026943695 28.51168450114867 20.934908810816292 14-15 22.710608161915836 25.803060169822434 27.467206831055535 24.0191248372062 16-17 21.176254665658558 29.063854120061468 27.929533026995667 21.83035818728431 18-19 21.013333323660465 27.197198108567306 31.101324989517025 20.688143578255197 20-21 22.193249372323773 26.43641866072402 30.859343756183584 20.510988210768627 22-23 21.83756425653966 26.551358159997807 30.68378445101937 20.92729313244316 24-25 21.428829310338884 26.692693805210833 30.929079585242143 20.949397299208137 26-27 21.2363001166403 26.897972120627372 30.798645891182524 21.0670818715498 28-29 21.111238501320305 26.989778628873378 30.84204041381823 21.056942455988093 30-31 21.2840203242211 26.885170907893084 30.749490116864997 21.08131865102082 32-33 21.09376686158317 27.003942765246137 30.596776033558935 21.305514339611758 34-35 21.53111579008615 26.99113419082218 30.399925087912607 21.077824931179066 36-37 21.687394623676283 26.78844660180027 30.254170971582457 21.269987802940992 38-39 21.3840020585968 27.03376124247171 30.312437228186095 21.269799470745397 40-41 21.457899413024165 26.8823827140714 30.384374499322718 21.275343373581716 42-43 21.37457756888022 26.993867600489253 30.020455901432857 21.611098929197667 44-45 21.34999337008202 27.141627433580606 29.92630226888907 21.5820769274483 46-47 21.53611278628377 27.28004760384335 29.451617785725514 21.732221824147366 48-49 21.635999263304647 27.440553840425302 29.070683587342028 21.852763308928022 50-51 21.524102524870287 27.692425389140407 28.86783843671704 21.915633649272266 52-53 21.678043718994548 27.69614098327388 28.77327668761735 21.85253861011422 54-55 21.346275183530487 27.60758646114749 28.842009320055606 22.204129035266416 56-57 21.433416768570563 27.91902077564723 28.659594247976568 21.987968207805643 58-59 21.534300058006394 27.425863423163744 28.826628612336318 22.213207906493547 60-61 21.51776535922398 27.47418773824743 29.05244501198335 21.955601890545235 62-63 21.403876770011507 27.35902281997703 29.149203451570244 22.08789695844122 64-65 21.231848848180825 27.352032005311138 29.082416153944397 22.333702992563637 66-67 21.221075085215414 27.500862220215687 28.812675848742614 22.46538684582628 68-69 21.24610116224449 27.310176390180047 28.912293961525272 22.53142848605019 70-71 21.160234131231924 27.291757578518233 28.86100166260505 22.687006627644795 72-73 21.22813710518712 27.28182834122749 28.6112650591215 22.87876949446388 74-75 21.057715166911535 27.45632627928774 28.544774046164484 22.94118450763624 76-77 21.103205037835863 27.239579519785277 28.653073951959584 23.004141490419276 78-79 21.056618450944587 27.299157202094506 28.60867693621001 23.0355474107509 80-81 21.076613475232193 27.246343980421834 28.491777085830027 23.185265458515943 82-83 21.141797597164548 27.10650596283249 28.458631341400093 23.293065098602874 84-85 20.95927154500921 26.932240412875665 28.48910205953391 23.619385982581214 86-87 21.021273448952176 26.75776070338842 28.617229460947314 23.60373638671209 88-89 21.096746422657016 26.564193252755793 28.573749835826494 23.765310488760697 90-91 20.95706946638824 26.587353852504542 28.67022151589162 23.7853551652156 92-93 21.122323484366692 26.433245349611873 28.518785638826856 23.925645527194586 94-95 20.964219653821566 26.167171219483848 28.60820849772974 24.26040062896485 96-97 21.137105832005663 25.940423779840714 28.502614805968733 24.419855582184887 98-99 20.983719623675697 25.910132934788866 28.45828654540791 24.647860896127526 100-101 21.207827511187467 25.42197989388846 28.81654375388884 24.553648841035237 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3273.0 1 2082.5 2 1285.5 3 1663.5 4 1414.0 5 1351.5 6 2118.0 7 3818.5 8 5711.5 9 6492.0 10 5878.0 11 5243.0 12 5421.5 13 5757.0 14 6086.5 15 6403.5 16 6691.5 17 6939.0 18 7249.0 19 7751.0 20 8328.5 21 9193.0 22 10367.5 23 11804.5 24 13358.5 25 15766.0 26 19568.5 27 23800.5 28 27224.5 29 31008.0 30 36224.0 31 41815.5 32 47658.5 33 52739.0 34 58242.0 35 63637.5 36 68944.0 37 74865.0 38 77880.5 39 77831.0 40 78460.0 41 81866.0 42 85062.5 43 89250.0 44 94163.0 45 97011.5 46 98316.5 47 98511.5 48 100812.5 49 101992.0 50 98736.0 51 94230.0 52 90399.0 53 87996.0 54 84848.0 55 78816.5 56 71169.0 57 62895.0 58 56664.0 59 50996.0 60 43535.0 61 35529.0 62 28716.5 63 22498.0 64 16446.0 65 12220.0 66 8912.0 67 6873.0 68 5172.0 69 3640.0 70 2718.5 71 2003.5 72 1562.5 73 1261.5 74 973.5 75 741.5 76 567.0 77 369.5 78 235.5 79 157.5 80 122.5 81 111.0 82 94.0 83 75.5 84 50.0 85 37.0 86 36.0 87 26.5 88 19.5 89 17.5 90 12.0 91 9.0 92 9.0 93 6.5 94 5.0 95 6.0 96 5.0 97 2.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16093683437718098 2 0.09259578170743867 3 0.08563478323922089 4 0.1012970297927109 5 0.12301389480553616 6 0.03792293957164481 7 0.04198352201143852 8 0.04542776604519211 9 0.04600784925087692 10-11 0.04439449283506603 12-13 0.04881762727841275 14-15 0.04664231525709469 16-17 0.045155852042527346 18-19 0.04977839008782822 20-21 0.05182680890790273 22-23 0.06502370183723227 24-25 0.08177360440138132 26-27 0.08398517162305469 28-29 0.07392435352445866 30-31 0.06203264780791995 32-33 0.0508841736986649 34-35 0.30218709496143353 36-37 0.48223041992585813 38-39 0.4591902401000643 40-41 0.43306836824407 42-43 0.46432035095033947 44-45 0.47468933825195553 46-47 0.47380108584325065 48-49 0.4907322644091762 50-51 0.47974693870151996 52-53 0.4874692963771991 54-55 0.4777347750818008 56-57 0.45618105847057433 58-59 0.4341922794550843 60-61 0.4017438751370899 62-63 0.4601510029094798 64-65 0.5005211685051075 66-67 0.5023701837232277 68-69 0.5125760226232451 70-71 0.5089323749875373 72-73 0.5000317233003109 74-75 0.49243625882587533 76-77 0.474145510246626 78-79 0.4485130835954282 80-81 0.45367944964605866 82-83 0.4656436657633079 84-85 0.46399405414714173 86-87 0.47541444225906154 88-89 0.4863816403665401 90-91 0.49029720200491256 92-93 0.4926537900280072 94-95 0.49725820047313035 96-97 0.49878091888805304 98-99 0.4920193240217894 100-101 0.47816983748606445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2758225.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.7479231378494 #Duplication Level Percentage of deduplicated Percentage of total 1 79.15194132424504 43.33404399831333 2 11.59972959423676 12.701222084902222 3 3.6799248547126515 6.044047292966096 4 1.6298425555597569 3.5692197983432643 5 0.933926342986034 2.556526382110608 6 0.5783245620354609 1.8997241202628696 7 0.41033270424007223 1.5725404346876952 8 0.28994982977103223 1.2699320795309592 9 0.22403742508340757 1.1039025355621281 >10 1.3321442529508465 13.617349678359588 >50 0.10839074445733177 4.10619643769883 >100 0.0590631095880035 5.7196813514955505 >500 0.0015286695297677753 0.5318047861892952 >1k 6.646389259596129E-4 0.6182253381502856 >5k 6.646389259596129E-5 0.30925821947847426 >10k+ 1.3292778519192257E-4 1.0463254619488795 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16291 0.590633468988208 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12464 0.45188481722847124 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8499 0.30813294781970285 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2798 0.1014420505941321 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.6255200355300965E-5 0.0 10-11 3.6255200355300965E-5 0.0 0.0 3.6255200355300965E-5 0.0 12-13 3.6255200355300965E-5 0.0 0.0 3.6255200355300965E-5 0.0 14-15 3.6255200355300965E-5 0.0 0.0 3.6255200355300965E-5 0.0 16-17 3.6255200355300965E-5 0.0 0.0 1.087656010659029E-4 0.0 18-19 7.251040071060193E-5 0.0 0.0 2.3565880230945626E-4 0.0 20-21 1.4502080142120386E-4 0.0 0.0 3.988072039083106E-4 0.0 22-23 1.8127600177650482E-4 0.0 0.0 9.78890409593126E-4 0.0 24-25 1.8127600177650482E-4 0.0 0.0 0.0017221220168767957 0.0 26-27 1.8127600177650482E-4 0.0 0.0 0.003988072039083106 0.0 28-29 1.8127600177650482E-4 0.0 0.0 0.011601664113696309 0.0 30-31 1.8127600177650482E-4 0.0 0.0 0.03322789112563333 0.0 32-33 2.175312021318058E-4 0.0 0.0 0.07448630912996583 0.0 34-35 2.175312021318058E-4 0.0 0.0 0.13896618296186858 0.0 36-37 2.175312021318058E-4 0.0 0.0 0.24122397556399494 0.0 38-39 2.175312021318058E-4 0.0 0.0 0.39351394465643663 0.0 40-41 2.175312021318058E-4 0.0 0.0 0.5710737883965231 0.0 42-43 2.3565880230945626E-4 0.0 0.0 0.7520053657696526 0.0 44-45 2.537864024871067E-4 0.0 0.0 0.9591857082000199 0.0 46-47 2.900416028424077E-4 1.8127600177650482E-5 0.0 1.2026031233855106 0.0 48-49 3.2629680319770865E-4 3.6255200355300965E-5 0.0 1.4673748515802734 0.0 50-51 3.2629680319770865E-4 3.6255200355300965E-5 0.0 1.7582503240308531 0.0 52-53 3.2629680319770865E-4 3.6255200355300965E-5 0.0 2.0451195968421723 0.0 54-55 3.2629680319770865E-4 3.6255200355300965E-5 0.0 2.356660533495273 0.0 56-57 3.2629680319770865E-4 3.6255200355300965E-5 0.0 2.7129766425871713 0.0 58-59 3.2629680319770865E-4 3.6255200355300965E-5 0.0 3.1043696580228226 0.0 60-61 3.2629680319770865E-4 7.251040071060193E-5 0.0 3.5406828666986923 0.0 62-63 3.2629680319770865E-4 7.251040071060193E-5 0.0 3.9796064498001433 0.0 64-65 3.2629680319770865E-4 7.251040071060193E-5 0.0 4.427394429388466 0.0 66-67 3.2629680319770865E-4 7.251040071060193E-5 0.0 4.925214585467103 0.0 68-69 3.988072039083106E-4 7.251040071060193E-5 0.0 5.470456543610474 0.0 70-71 3.988072039083106E-4 7.251040071060193E-5 0.0 6.074341288328545 0.0 72-73 3.988072039083106E-4 7.251040071060193E-5 0.0 6.675796934622809 0.0 74-75 3.988072039083106E-4 7.251040071060193E-5 0.0 7.291917809460795 0.0 76-77 3.988072039083106E-4 7.251040071060193E-5 0.0 7.947901277089432 0.0 78-79 3.988072039083106E-4 7.251040071060193E-5 0.0 8.658611969654398 0.0 80-81 4.350624042636116E-4 7.251040071060193E-5 0.0 9.41948173191092 0.0 82-83 4.350624042636116E-4 7.251040071060193E-5 0.0 10.20785106363694 0.0 84-85 4.350624042636116E-4 7.251040071060193E-5 0.0 11.01287965992622 0.0 86-87 4.350624042636116E-4 7.251040071060193E-5 0.0 11.826174441896509 0.0 88-89 4.350624042636116E-4 7.251040071060193E-5 0.0 12.679567475459761 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8355 0.0 47.71925 1 GTATCAA 14405 0.0 41.401054 1 ATCAACG 18535 0.0 31.825586 3 TATCAAC 18640 0.0 31.697147 2 TCAACGC 18690 0.0 31.536297 4 CAACGCA 18925 0.0 31.094627 5 AACGCAG 19505 0.0 30.268929 6 GTACATG 14070 0.0 26.955038 1 ACGCAGA 21805 0.0 26.729227 7 CGCAGAG 21990 0.0 26.439764 8 TACATGG 14345 0.0 25.961012 2 ACATGGG 14430 0.0 24.757378 3 GCAGAGT 23105 0.0 24.324116 9 GAGTACT 13140 0.0 22.322672 12-13 CATGGGA 8670 0.0 21.367676 4 AGTACTT 13585 0.0 21.330055 12-13 AGAGTAC 21125 0.0 20.832674 10-11 CATGGGG 5590 0.0 20.68129 4 CAGAGTA 22720 0.0 20.412136 10-11 GTACTTT 14975 0.0 19.746828 14-15 >>END_MODULE