FastQCFastQC Report
Thu 26 May 2016
SRR938787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758225
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153840.5577500022659501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128040.46421158534927354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91030.33003108883430465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29050.1053213570321493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83000.048.895241
GTATCAA143300.040.3864021
ATCAACG190550.030.1810553
TATCAAC191650.029.9830672
TCAACGC192950.029.7810574
CAACGCA196250.029.280285
AACGCAG201000.028.6119386
GTACATG149250.026.0104791
TACATGG150950.025.3991372
ACGCAGA230900.024.6191837
CGCAGAG231500.024.432398
GTGGTAT42450.024.3493371
ACATGGG150950.024.3303623
TGGTATC43700.023.019462
GCAGAGT244900.022.5146889
CATGGGA91950.022.4996364
CATGGGG57700.021.5459794
GAGTACT137300.021.35829412-13
AGTACTT142650.021.20591412-13
AGAGTAC223650.020.28325310-11
Produced by FastQC (version 0.11.5)