##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938787_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2758225 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98329795430032 33.0 31.0 34.0 30.0 34.0 2 32.19211558157873 34.0 31.0 34.0 30.0 34.0 3 32.27630160701175 34.0 31.0 34.0 30.0 34.0 4 35.92896192297583 37.0 35.0 37.0 35.0 37.0 5 35.83907077921489 37.0 35.0 37.0 35.0 37.0 6 35.86707429596933 37.0 35.0 37.0 35.0 37.0 7 35.79696834014629 37.0 35.0 37.0 33.0 37.0 8 35.76698057627641 37.0 35.0 37.0 33.0 37.0 9 37.41091970379501 39.0 37.0 39.0 34.0 39.0 10-11 37.3413976379737 39.0 37.0 39.0 34.0 39.0 12-13 37.273135077812725 39.0 37.0 39.0 33.0 39.0 14-15 38.47299495146335 40.0 38.0 41.0 33.5 41.0 16-17 38.41660995749078 40.0 38.0 41.0 33.0 41.0 18-19 38.459772136065766 40.0 38.0 41.0 33.5 41.0 20-21 38.52364382891171 40.0 38.0 41.0 34.0 41.0 22-23 38.46047059250061 40.0 38.0 41.0 34.0 41.0 24-25 38.38540746313299 40.0 38.0 41.0 34.0 41.0 26-27 38.21827243970307 40.0 38.0 41.0 33.0 41.0 28-29 38.04717581053032 40.0 37.0 41.0 33.0 41.0 30-31 37.78942453933236 40.0 37.0 41.0 33.0 41.0 32-33 37.52954889466958 40.0 36.0 41.0 32.0 41.0 34-35 37.4513772444235 40.0 36.0 41.0 32.0 41.0 36-37 37.33941919169031 40.0 36.0 41.0 32.0 41.0 38-39 37.22146144712632 40.0 36.0 41.0 31.0 41.0 40-41 37.042583364301315 40.0 36.0 41.0 31.0 41.0 42-43 36.83560731992495 40.0 36.0 41.0 31.0 41.0 44-45 36.53255716991906 39.5 35.0 41.0 30.0 41.0 46-47 36.152115219026726 39.0 35.0 41.0 29.5 41.0 48-49 35.80309021200229 39.0 35.0 41.0 28.0 41.0 50-51 35.541441325490126 39.0 35.0 41.0 27.0 41.0 52-53 35.32777384006019 39.0 35.0 40.5 27.0 41.0 54-55 35.08262088843368 38.5 35.0 40.0 26.0 41.0 56-57 34.82601781943097 38.0 34.0 40.0 26.0 41.0 58-59 34.51825757507092 38.0 34.0 40.0 25.0 41.0 60-61 34.18812134615559 37.5 34.0 40.0 24.5 41.0 62-63 33.82467474553381 37.0 33.0 40.0 23.0 41.0 64-65 33.4618163855378 36.5 33.0 39.5 22.5 41.0 66-67 33.07364827017375 36.0 33.0 39.0 22.0 40.5 68-69 32.634338387912514 35.5 32.0 39.0 20.5 40.0 70-71 32.17963708544444 35.0 32.0 38.0 19.5 40.0 72-73 31.850190792991867 35.0 31.5 37.5 18.0 40.0 74-75 31.43720998105666 35.0 31.0 37.0 16.5 39.0 76-77 29.37961025659618 33.0 28.5 35.0 10.5 37.5 78-79 30.425826029421096 34.0 30.5 36.0 10.0 38.0 80-81 30.474073362397917 34.0 31.0 36.0 7.5 37.0 82-83 30.169520071785296 34.0 30.5 35.0 6.0 37.0 84-85 29.794720699000262 34.0 30.0 35.0 2.0 36.5 86-87 29.27815189116189 34.0 29.5 35.0 2.0 36.0 88-89 28.782575931985242 34.0 29.0 35.0 2.0 36.0 90-91 28.381296485964704 34.0 29.0 35.0 2.0 35.0 92-93 28.108764694685895 34.0 29.0 35.0 2.0 35.0 94-95 27.743282908392175 34.0 27.5 35.0 2.0 35.0 96-97 27.262570131153186 33.0 27.0 35.0 2.0 35.0 98-99 26.588760344061853 33.0 25.5 35.0 2.0 35.0 100-101 23.673885016632074 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04587268790693955 1101 2 0.08169615916150264 1101 3 0.0784408210514016 1101 4 0.09247617354414217 1101 5 0.08542557121000272 1101 6 0.1316685625558236 1101 7 0.10041357454492328 1101 8 0.12233616250264134 1101 9 0.18482464381907704 1101 10-11 0.14187203442654805 1101 12-13 0.18369396080873912 1101 14-15 0.1688566569255343 1101 16-17 0.18142981492139398 1101 18-19 0.24648982782577633 1101 20-21 0.15455065703488913 1101 22-23 0.17874331491078266 1101 24-25 0.11957216596612597 1101 26-27 0.22209354546217241 1101 28-29 0.2590175702895152 1101 30-31 0.18370270996546623 1101 32-33 0.23014258934748 1101 34-35 0.2341781061212984 1101 36-37 0.22491982797040322 1101 38-39 0.19191682915366215 1101 40-41 0.28132672540570525 1101 42-43 0.19215628876045798 1101 44-45 0.37100895863170535 1101 46-47 0.46075780910728525 1101 48-49 0.2701476207979283 1101 50-51 0.29683737504431207 1101 52-53 0.42986702155840817 1101 54-55 0.31608065906877414 1101 56-57 0.2502896180684715 1101 58-59 0.20459926120198446 1101 60-61 0.3112939168729838 1101 62-63 0.2482008137344991 1101 64-65 0.24332607548299023 1101 66-67 0.09335845322018343 1101 68-69 0.15720344981564693 1101 70-71 0.1994002900605807 1101 72-73 0.19974492075043315 1101 74-75 0.2632385170403211 1101 76-77 0.09178394792230549 1101 78-79 0.17978806902697286 1101 80-81 0.24834606217440225 1101 82-83 0.3428270122598214 1101 84-85 0.2912385857175366 1101 86-87 0.3987568863807809 1101 88-89 0.45133469665751136 1101 90-91 0.46223645869356744 1101 92-93 0.3478065846370342 1101 94-95 0.3140895474933423 1101 96-97 0.2090410868681012 1101 98-99 0.26540806030790876 1101 100-101 0.8151098505046797 1102 1 0.05935222206042923 1102 2 0.03934898108121132 1102 3 0.12135370599962414 1102 4 0.09131644401779226 1102 5 0.046526677915927905 1102 6 0.0892306521576458 1102 7 0.12231873095694112 1102 8 0.1439811485601581 1102 9 0.22983228832132596 1102 10-11 0.2317733752096771 1102 12-13 0.2006058711457186 1102 14-15 0.16849913201182432 1102 16-17 0.18969231691377075 1102 18-19 0.2059781523576234 1102 20-21 0.25963573152291985 1102 22-23 0.1803217679947906 1102 24-25 0.13658242039263513 1102 26-27 0.28210437244614184 1102 28-29 0.2244208503237033 1102 30-31 0.1885980686572637 1102 32-33 0.2863233250800121 1102 34-35 0.22150130940446644 1102 36-37 0.24365095172822038 1102 38-39 0.21854265409844942 1102 40-41 0.20207684114269853 1102 42-43 0.13962714823451705 1102 44-45 0.11099510216261876 1102 46-47 -0.032284422676340796 1102 48-49 0.0015206641349436723 1102 50-51 -0.11944714262741485 1102 52-53 -0.11352060958670762 1102 54-55 0.0731110816454077 1102 56-57 -0.07802420857769476 1102 58-59 -0.061660358364711954 1102 60-61 -0.1432659377604466 1102 62-63 -0.16954105373450545 1102 64-65 -0.0999846158744333 1102 66-67 -0.1613367539364603 1102 68-69 -0.2254852958602953 1102 70-71 -0.2426978004047129 1102 72-73 -0.19802327735902026 1102 74-75 -0.19693654152845497 1102 76-77 -0.2226928196566611 1102 78-79 -0.23402422133532141 1102 80-81 -0.09667461368089292 1102 82-83 -0.11589480686835074 1102 84-85 -0.0994273727498971 1102 86-87 -0.10254929363183152 1102 88-89 0.09947415892846934 1102 90-91 0.07153746513723647 1102 92-93 0.03211010677123127 1102 94-95 0.03689554185484312 1102 96-97 0.06383847444718427 1102 98-99 -0.016464749119361244 1102 100-101 0.42255236678587593 1103 1 0.04971609271624189 1103 2 0.05083110648388356 1103 3 0.019945842295769012 1103 4 0.06834785497211726 1103 5 0.10656074357414269 1103 6 0.06783564759651028 1103 7 0.09422114508296886 1103 8 0.13099709443567065 1103 9 0.184305747116575 1103 10-11 0.15084754086881702 1103 12-13 0.1325138980571765 1103 14-15 0.19580124916286223 1103 16-17 0.07285041525659608 1103 18-19 0.13053630257981297 1103 20-21 0.224770266339398 1103 22-23 0.0426124815575335 1103 24-25 0.08080267255509455 1103 26-27 0.07014475637741668 1103 28-29 0.21595103445358177 1103 30-31 0.24738249670814838 1103 32-33 0.21128725036773943 1103 34-35 0.3266571135661209 1103 36-37 0.2763524577558272 1103 38-39 0.34384247886345065 1103 40-41 0.2943827774411716 1103 42-43 0.21058422987331937 1103 44-45 0.18794217174111338 1103 46-47 0.2315593510063323 1103 48-49 0.3869855843756511 1103 50-51 0.33866557622560123 1103 52-53 0.2541325430125383 1103 54-55 0.25362930090845026 1103 56-57 0.2522458782978063 1103 58-59 0.05907391723442146 1103 60-61 0.09928635075108616 1103 62-63 0.191780287505793 1103 64-65 0.23897504118317414 1103 66-67 0.11992071643859248 1103 68-69 0.09630742062313402 1103 70-71 0.1661930940476921 1103 72-73 0.16358745443032063 1103 74-75 0.21245235299516096 1103 76-77 0.08586646868043957 1103 78-79 0.07938133023603555 1103 80-81 0.29783082542532213 1103 82-83 0.2624467975445661 1103 84-85 0.27777925823549054 1103 86-87 0.4943143381215762 1103 88-89 0.6587982491171971 1103 90-91 0.67838899768795 1103 92-93 0.6835918851146765 1103 94-95 0.5851229368552175 1103 96-97 0.55495719892075 1103 98-99 0.49077869108727157 1103 100-101 0.9756736708669074 1104 1 -0.04334950763085743 1104 2 0.05944707020243811 1104 3 0.015275515041587084 1104 4 0.024468589864866885 1104 5 0.03754889699047936 1104 6 0.060927632317024916 1104 7 -0.0075383994641171626 1104 8 0.047826513598351994 1104 9 -0.011077573855189371 1104 10-11 0.07894748487532155 1104 12-13 0.09705139464919199 1104 14-15 0.05676908661167346 1104 16-17 0.10090550582828683 1104 18-19 0.12576351600886682 1104 20-21 0.07659225390356283 1104 22-23 -0.03544759272913467 1104 24-25 -0.04142498940831274 1104 26-27 -0.08767044160798321 1104 28-29 -0.04915211378848028 1104 30-31 -0.137273084612886 1104 32-33 -0.09089898812941755 1104 34-35 -0.09696144461582179 1104 36-37 -0.21232380891396474 1104 38-39 0.020118822927408075 1104 40-41 -0.08279444639505584 1104 42-43 -0.037484873732246626 1104 44-45 -0.03768892781299371 1104 46-47 -0.036114221525771484 1104 48-49 -0.24922887367466728 1104 50-51 -0.16882641318547797 1104 52-53 -0.2180567150295687 1104 54-55 -0.24023032249859 1104 56-57 -0.25099016510154826 1104 58-59 -0.17322847113769058 1104 60-61 -0.17809989595453857 1104 62-63 -0.5182397646180021 1104 64-65 -0.158733182826019 1104 66-67 -0.3608147333123668 1104 68-69 -0.3491219264222565 1104 70-71 -0.3989461128380434 1104 72-73 -0.4787442205962833 1104 74-75 -0.4555564476591698 1104 76-77 -0.3759840726389534 1104 78-79 -0.26272871605388204 1104 80-81 -0.22718463874909745 1104 82-83 -0.29892633319586537 1104 84-85 -0.34961475720229984 1104 86-87 0.009204359258390582 1104 88-89 0.020531966294303317 1104 90-91 0.0061754073903692586 1104 92-93 -0.07396196099407248 1104 94-95 -0.05598303744640276 1104 96-97 -0.10470358598503893 1104 98-99 -0.07417347990692136 1104 100-101 0.465948097666395 1105 1 -0.01553934004882862 1105 2 0.034719567033867804 1105 3 0.03541915678218999 1105 4 -0.019498457029939686 1105 5 -0.0025454559175628333 1105 6 -0.0079443575435576 1105 7 0.031232196226632425 1105 8 -0.07914996858725942 1105 9 0.069727946494055 1105 10-11 0.1118527094915649 1105 12-13 0.07511434352962709 1105 14-15 0.0239145590803318 1105 16-17 -0.039421208718955825 1105 18-19 -0.0511052348712866 1105 20-21 -0.08508638290815895 1105 22-23 -0.05529479734820342 1105 24-25 -0.09897744680867504 1105 26-27 -0.0683914668157044 1105 28-29 -0.007047480638220804 1105 30-31 -0.04421506461570601 1105 32-33 0.03157038238177989 1105 34-35 0.0813754093484107 1105 36-37 0.07454001308293101 1105 38-39 0.05643835538762687 1105 40-41 0.028935591627757162 1105 42-43 -0.00983454279294449 1105 44-45 0.07618869505702719 1105 46-47 0.24911039472545582 1105 48-49 0.1452071212556234 1105 50-51 0.2368896831389904 1105 52-53 0.19993582123293407 1105 54-55 0.19258829459759141 1105 56-57 0.14639048551103429 1105 58-59 0.19901980375316697 1105 60-61 0.09560592012770996 1105 62-63 0.08864870179517936 1105 64-65 0.06325386125227794 1105 66-67 0.07174758185450969 1105 68-69 0.1579967892515981 1105 70-71 0.07518992433981708 1105 72-73 -0.03582843408734249 1105 74-75 -0.09522762073338598 1105 76-77 -0.17951104067162404 1105 78-79 -0.16958408853663443 1105 80-81 -0.03320632583542249 1105 82-83 -0.06120136488154415 1105 84-85 0.046864611900641506 1105 86-87 0.0626686600610924 1105 88-89 0.13619666140766995 1105 90-91 0.2466739287253894 1105 92-93 0.24025997957767942 1105 94-95 0.18117722167203354 1105 96-97 0.18473874608086405 1105 98-99 0.3088099027152431 1105 100-101 0.7216479990887272 1106 1 -0.07294300634138295 1106 2 -0.08740164508733983 1106 3 -0.04244092552233525 1106 4 -0.004186073939777657 1106 5 -0.019406660556541055 1106 6 -0.07319688279608272 1106 7 -0.05901471735361241 1106 8 -0.02307813918209689 1106 9 -0.04501204602371445 1106 10-11 0.0768807678529626 1106 12-13 0.046691223439843554 1106 14-15 0.0558449743440832 1106 16-17 -0.03207740026403627 1106 18-19 -0.023744591399527337 1106 20-21 0.023046800780583965 1106 22-23 -0.07364309514094458 1106 24-25 -0.10244171916183831 1106 26-27 0.054335805911563284 1106 28-29 0.03995775692256842 1106 30-31 0.07858141873633429 1106 32-33 -0.03947900033426066 1106 34-35 0.056998304971308755 1106 36-37 0.09702037222996296 1106 38-39 0.0657107085488704 1106 40-41 0.08563779888552148 1106 42-43 0.043425838800772 1106 44-45 0.10262528316027897 1106 46-47 0.11114182009021789 1106 48-49 0.10434666483961053 1106 50-51 0.10867419492350194 1106 52-53 0.1314004676975813 1106 54-55 0.21816442628483657 1106 56-57 0.1687062356045672 1106 58-59 0.19948557255226973 1106 60-61 0.19251762981719622 1106 62-63 0.1685607855961493 1106 64-65 0.16374394729792385 1106 66-67 0.14379210112124952 1106 68-69 0.06536678551047714 1106 70-71 0.09297144323245021 1106 72-73 0.14247871144202406 1106 74-75 0.22406343800211914 1106 76-77 0.06743433905153395 1106 78-79 0.16836578052434703 1106 80-81 0.12308997046087455 1106 82-83 0.33692620713491905 1106 84-85 0.22944972226353144 1106 86-87 0.3462825770083455 1106 88-89 0.4515707728928895 1106 90-91 0.5647285490022291 1106 92-93 0.5852581090202555 1106 94-95 0.743532253471507 1106 96-97 0.7461154763464855 1106 98-99 0.6792569659400876 1106 100-101 1.2067115980412133 1107 1 -0.14933411843377087 1107 2 -0.12737515063462013 1107 3 -0.1226136301030607 1107 4 -0.12935273753995347 1107 5 -0.07548413229886108 1107 6 -0.045940714556309104 1107 7 -0.07075323182436932 1107 8 -0.06183363586109181 1107 9 0.027044279220220346 1107 10-11 0.015179971179485108 1107 12-13 -0.17037745974905505 1107 14-15 -0.2140028665420104 1107 16-17 -0.08303383774142503 1107 18-19 -0.04268689850213292 1107 20-21 -0.12828006839691852 1107 22-23 -0.1252735465892485 1107 24-25 -0.20689487243102178 1107 26-27 -0.22215588697356736 1107 28-29 -0.2519442626661572 1107 30-31 -0.2866771168318536 1107 32-33 -0.1760707833741364 1107 34-35 -0.2782724352174668 1107 36-37 -0.23486496566630422 1107 38-39 -0.30014027242051355 1107 40-41 -0.3238057562775225 1107 42-43 -0.30240588462658025 1107 44-45 -0.31966133530120544 1107 46-47 -0.2753036914919065 1107 48-49 -0.5590916036988318 1107 50-51 -0.5972815372617433 1107 52-53 -0.5530726790706453 1107 54-55 -0.5807966477217761 1107 56-57 -0.5852124288787621 1107 58-59 -0.545694828912282 1107 60-61 -0.46568430452317244 1107 62-63 -0.49629665339607953 1107 64-65 -0.3774322346797163 1107 66-67 -0.5960575183276333 1107 68-69 -0.42615621621288824 1107 70-71 -0.5647190009789398 1107 72-73 -0.5247871468985146 1107 74-75 -0.45847291520819056 1107 76-77 -0.49941693016767985 1107 78-79 -0.4944414048693595 1107 80-81 -0.5280940003952246 1107 82-83 -0.41723961624766304 1107 84-85 -0.5372580353367589 1107 86-87 -0.42002715050357153 1107 88-89 -0.34054590082669733 1107 90-91 -0.17522042464437604 1107 92-93 -0.20787602527963855 1107 94-95 0.015072182206335327 1107 96-97 -0.10036143606849635 1107 98-99 -0.017544074212501926 1107 100-101 0.24030312962910116 1108 1 -0.129271779384716 1108 2 -0.056674111127051674 1108 3 -0.07292375509520355 1108 4 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13 12859.0 14 14158.0 15 18207.0 16 22945.0 17 31486.0 18 29315.0 19 18378.0 20 16383.0 21 16947.0 22 17836.0 23 19517.0 24 22697.0 25 26513.0 26 31603.0 27 38400.0 28 46639.0 29 57013.0 30 69603.0 31 83971.0 32 103114.0 33 129365.0 34 171176.0 35 237860.0 36 354866.0 37 564049.0 38 521070.0 39 58401.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69582147780138 20.21758730627876 14.854889016933285 22.23170219898657 2 15.707783085136274 22.19503485031134 39.20822267944058 22.8889593851118 3 18.191844392680075 26.848281050313155 31.501636015916034 23.458238541090736 4 12.886330883086043 18.158489608353197 37.61038348938176 31.344796019179 5 12.836878789801412 38.41002818841828 34.248402505234345 14.504690516545967 6 29.480154809705517 37.345502995585925 18.354195179871112 14.82014701483744 7 26.414632598863395 34.49780202847846 21.92685513328318 17.16071023937496 8 25.892920265750618 34.62273744890283 20.945499370065892 18.53884291528066 9 26.752386045373385 16.601691305096576 21.230066437654653 35.41585621187539 10-11 24.150785378277696 27.241359932565327 27.958324647191578 20.649530041965395 12-13 25.391148292833254 25.17920039155616 28.45935338850166 20.97029792710892 14-15 22.519505333149638 25.98735778871881 27.385270210498074 24.107866667633473 16-17 21.056494759093795 29.246868880087927 27.87292680968117 21.823709551137107 18-19 20.968255064544355 27.30043783680015 30.957652210057972 20.773654888597516 20-21 22.112975212858853 26.500797886466458 30.788737464988802 20.597489435685894 22-23 21.726055706115346 26.721768528673334 30.628882705363054 20.923293059848273 24-25 21.412194436639506 26.82355500367084 30.842480218256306 20.921770341433348 26-27 21.233529927335447 27.0124146982341 30.770740939137433 20.983314435293018 28-29 21.050711961496976 27.073860906923837 30.800424185844154 21.07500294573503 30-31 21.333810693471346 26.93400647155326 30.712577835383264 21.01960499959213 32-33 21.1614897261826 27.0698365796844 30.555701583445877 21.212972110687126 34-35 21.523400739049904 27.064607131144463 30.413428133896275 20.998563995909354 36-37 21.637860871699914 26.868956009238694 30.250028147951802 21.243154971109597 38-39 21.32439657383931 27.05214751454686 30.37573006248419 21.247725849129637 40-41 21.429577588627744 26.889690956579027 30.41266521646672 21.268066238326504 42-43 21.390556583021922 27.03312349331958 30.051197676109165 21.525122247549337 44-45 21.338782128035557 27.25587152465762 29.82049468786024 21.584851659446585 46-47 21.572035492944266 27.304905912662765 29.403226473089884 21.71983212130309 48-49 21.627409226478843 27.467760510429805 29.017163033683445 21.887667229407903 50-51 21.564099087307948 27.734795052160564 28.819935654013797 21.881170206517698 52-53 21.694495554206057 27.768238631728735 28.70525428491149 21.832011529153714 54-55 21.353642288065693 27.69893681624958 28.681887808282504 22.265533087402225 56-57 21.440455365316463 27.993310915534448 28.482810503131546 22.083423216017547 58-59 21.499837847822764 27.526913726521695 28.701787371998122 22.271461053657415 60-61 21.449400207308887 27.691725373368094 28.847912934617213 22.010961484705803 62-63 21.349926130029274 27.58839471036627 28.858903824017258 22.202775335587198 64-65 21.141331834785053 27.571971104605318 28.73792022043162 22.548776840178014 66-67 21.08010570688853 27.80824257040751 28.504495515723523 22.607156206980438 68-69 21.14980017772454 27.63328343365839 28.547973842370446 22.66894254624663 70-71 21.119815761766507 27.648007663057506 28.40450617946405 22.827670395711934 72-73 21.192832346889755 27.692193348983494 28.125279844827745 22.989694459299006 74-75 21.07552864614018 27.851063636940427 27.971775326523396 23.101632390396 76-77 21.098150254760277 27.663850352403713 28.218681379899024 23.019318012936985 78-79 21.071777460187757 27.718046795988794 28.056158668312527 23.15401707551093 80-81 21.064688341233946 27.76347107288202 27.903216742651527 23.268623843232515 82-83 21.132376800297294 27.656790145836545 27.838301806415355 23.372531247450805 84-85 20.955777719366623 27.501853547118166 27.828132222715695 23.71423651079952 86-87 21.02236406644279 27.365703130148688 27.871923359096108 23.740009444312417 88-89 21.06591170028751 27.323828599061965 27.735229988125905 23.87502971252462 90-91 20.94445845131369 27.310007927261875 27.86168064132112 23.883852980103317 92-93 20.98724723327502 27.28760344061851 27.647907621749496 24.077241704356968 94-95 20.811467666971996 27.039205836943292 27.70981839948126 24.43950809660345 96-97 21.021494080703004 26.909194664901282 27.60293804945899 24.466373204936726 98-99 20.804490727161788 26.922772936284563 27.559506739317463 24.713229597236186 100-101 20.869558596826053 26.599899789863258 27.942748252123483 24.587793361187206 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2139.0 1 1469.0 2 1156.5 3 1528.0 4 1322.0 5 1269.0 6 1908.5 7 3432.5 8 5136.5 9 5740.0 10 5143.5 11 4516.0 12 4607.5 13 4929.5 14 5227.0 15 5516.0 16 5844.0 17 6147.5 18 6524.5 19 6971.0 20 7618.5 21 8675.5 22 9941.0 23 11441.5 24 13175.0 25 15674.5 26 19474.5 27 23825.0 28 27287.5 29 30962.0 30 36415.5 31 42123.0 32 47822.5 33 52706.5 34 58133.5 35 63719.0 36 69195.5 37 75021.5 38 77796.0 39 78114.0 40 78678.0 41 81861.5 42 85696.0 43 90326.5 44 95133.5 45 98026.5 46 99211.5 47 99575.0 48 102593.0 49 103745.5 50 100280.0 51 96250.5 52 92269.0 53 89392.5 54 86134.0 55 79466.0 56 71776.0 57 63427.5 58 56863.0 59 51110.0 60 43237.5 61 35122.5 62 28313.0 63 21914.5 64 15831.5 65 11601.5 66 8248.5 67 6335.0 68 4678.5 69 3164.0 70 2354.0 71 1737.5 72 1387.5 73 1156.0 74 890.0 75 666.5 76 448.5 77 253.5 78 156.5 79 99.5 80 62.0 81 45.5 82 31.0 83 25.0 84 17.0 85 11.5 86 12.0 87 8.0 88 4.0 89 5.0 90 5.0 91 3.0 92 2.0 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3956892566777547 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.8127600177650482E-4 16-17 0.002410970823627514 18-19 1.994036019541553E-4 20-21 1.8127600177650482E-5 22-23 0.0 24-25 0.0 26-27 9.063800088825241E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008139292479765066 36-37 0.01723934776894561 38-39 0.011873578116361066 40-41 0.007776740476212057 42-43 0.003335478432687689 44-45 0.0022296948218510095 46-47 0.0015589736152779414 48-49 1.8127600177650482E-5 50-51 3.988072039083106E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.89445204796563E-4 60-61 7.976144078166212E-4 62-63 0.0 64-65 0.0 66-67 0.0011601664113696309 68-69 8.701248085272232E-4 70-71 0.002066546420252155 72-73 0.0 74-75 0.0 76-77 0.10120639179182264 78-79 0.036309583155833915 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.15178239628746748 88-89 0.32497348838474016 90-91 0.27519509829691197 92-93 0.0 94-95 1.8127600177650482E-5 96-97 1.8127600177650482E-5 98-99 2.900416028424077E-4 100-101 7.613592074613202E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2758225.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.90505889035253 #Duplication Level Percentage of deduplicated Percentage of total 1 77.72042566437257 41.11803696756897 2 12.331502929037983 13.047977773346187 3 3.960349903174376 6.285676345615269 4 1.789980766248845 3.7879615140397394 5 0.9915047053784949 2.6227807414055455 6 0.6101627199614192 1.9368416779353939 7 0.41912247145193604 1.5521589324104343 8 0.3156427014911258 1.3359276568558378 9 0.24928318030980737 1.1869507201198255 >10 1.4293020488536265 14.171801289827894 >50 0.11615450509890139 4.2659342348990155 >100 0.06348509573486597 5.947600572602398 >500 0.0021240572327740686 0.7082059961366931 >1k 7.536977277366208E-4 0.6803663992000771 >5k 6.85179752487837E-5 0.32997897234366785 >10k+ 1.370359504975674E-4 1.0218002056929922 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15384 0.5577500022659501 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12804 0.46421158534927354 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9103 0.33003108883430465 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2905 0.1053213570321493 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.6255200355300965E-5 0.0 0.0 0.0 5 7.251040071060193E-5 3.6255200355300965E-5 0.0 0.0 0.0 6 7.251040071060193E-5 3.6255200355300965E-5 0.0 0.0 0.0 7 7.251040071060193E-5 3.6255200355300965E-5 0.0 0.0 0.0 8 7.251040071060193E-5 3.6255200355300965E-5 0.0 0.0 0.0 9 7.251040071060193E-5 3.6255200355300965E-5 0.0 3.6255200355300965E-5 0.0 10-11 7.251040071060193E-5 3.6255200355300965E-5 0.0 3.6255200355300965E-5 0.0 12-13 1.4502080142120386E-4 3.6255200355300965E-5 0.0 3.6255200355300965E-5 0.0 14-15 1.4502080142120386E-4 3.6255200355300965E-5 0.0 3.6255200355300965E-5 0.0 16-17 1.4502080142120386E-4 3.6255200355300965E-5 0.0 1.087656010659029E-4 0.0 18-19 1.4502080142120386E-4 3.6255200355300965E-5 0.0 2.3565880230945626E-4 0.0 20-21 1.4502080142120386E-4 3.6255200355300965E-5 0.0 4.1693480408596105E-4 0.0 22-23 1.4502080142120386E-4 3.6255200355300965E-5 0.0 0.0010151456099484271 0.0 24-25 1.4502080142120386E-4 3.6255200355300965E-5 0.0 0.0017583772172320967 0.0 26-27 1.6314840159885433E-4 3.6255200355300965E-5 0.0 0.004024327239438407 0.0 28-29 1.8127600177650482E-4 3.6255200355300965E-5 0.0 0.011782940115472814 0.0 30-31 1.8127600177650482E-4 3.6255200355300965E-5 0.0 0.03373546393060755 0.0 32-33 1.8127600177650482E-4 3.6255200355300965E-5 0.0 0.07604528274524378 0.0 34-35 2.3565880230945626E-4 3.6255200355300965E-5 0.0 0.14192098179082563 0.0 36-37 2.900416028424077E-4 3.6255200355300965E-5 0.0 0.24584651360929582 0.0 38-39 3.6255200355300965E-4 3.6255200355300965E-5 0.0 0.40123630233211577 0.0 40-41 3.6255200355300965E-4 3.6255200355300965E-5 0.0 0.5811708616954745 0.0 42-43 4.169348040859611E-4 3.6255200355300965E-5 0.0 0.7641871130890336 0.0 44-45 4.713176046189125E-4 3.6255200355300965E-5 0.0 0.9742497439476474 0.0 46-47 5.075728049742135E-4 3.6255200355300965E-5 0.0 1.2210207651660037 0.0 48-49 5.075728049742135E-4 3.6255200355300965E-5 0.0 1.4885297609875918 0.0 50-51 5.075728049742135E-4 3.6255200355300965E-5 0.0 1.7829038602724578 0.0 52-53 5.075728049742135E-4 3.6255200355300965E-5 0.0 2.072002827905628 0.0 54-55 5.075728049742135E-4 3.6255200355300965E-5 0.0 2.387531836597812 0.0 56-57 5.075728049742135E-4 3.6255200355300965E-5 0.0 2.747328444923819 0.0 58-59 5.075728049742135E-4 3.6255200355300965E-5 0.0 3.1434165088054815 0.0 60-61 5.075728049742135E-4 3.6255200355300965E-5 0.0 3.584297872726119 0.0 62-63 5.075728049742135E-4 3.6255200355300965E-5 0.0 4.027445186668963 0.0 64-65 5.257004051518639E-4 3.6255200355300965E-5 0.0 4.480272639106672 0.0 66-67 5.800832056848154E-4 3.6255200355300965E-5 0.0 4.982660950430077 0.0 68-69 5.800832056848154E-4 3.6255200355300965E-5 0.0 5.534247568635626 0.0 70-71 5.800832056848154E-4 3.6255200355300965E-5 0.0 6.144313825014276 0.0 72-73 5.800832056848154E-4 3.6255200355300965E-5 0.0 6.7536005945852855 0.0 74-75 5.800832056848154E-4 3.6255200355300965E-5 0.0 7.379310969917247 0.0 76-77 5.800832056848154E-4 3.6255200355300965E-5 0.0 8.04350624042636 0.0 78-79 5.800832056848154E-4 3.6255200355300965E-5 0.0 8.760344061851372 0.0 80-81 6.344660062177668E-4 3.6255200355300965E-5 0.0 9.528319843377535 0.0 82-83 6.888488067507184E-4 3.6255200355300965E-5 0.0 10.325064126385628 3.6255200355300965E-5 84-85 6.888488067507184E-4 3.6255200355300965E-5 0.0 11.136056703133356 3.6255200355300965E-5 86-87 6.888488067507184E-4 3.6255200355300965E-5 0.0 11.955442358763335 3.6255200355300965E-5 88-89 6.888488067507184E-4 3.6255200355300965E-5 0.0 12.8164489844012 3.6255200355300965E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8300 0.0 48.89524 1 GTATCAA 14330 0.0 40.386402 1 ATCAACG 19055 0.0 30.181055 3 TATCAAC 19165 0.0 29.983067 2 TCAACGC 19295 0.0 29.781057 4 CAACGCA 19625 0.0 29.28028 5 AACGCAG 20100 0.0 28.611938 6 GTACATG 14925 0.0 26.010479 1 TACATGG 15095 0.0 25.399137 2 ACGCAGA 23090 0.0 24.619183 7 CGCAGAG 23150 0.0 24.43239 8 GTGGTAT 4245 0.0 24.349337 1 ACATGGG 15095 0.0 24.330362 3 TGGTATC 4370 0.0 23.01946 2 GCAGAGT 24490 0.0 22.514688 9 CATGGGA 9195 0.0 22.499636 4 CATGGGG 5770 0.0 21.545979 4 GAGTACT 13730 0.0 21.358294 12-13 AGTACTT 14265 0.0 21.205914 12-13 AGAGTAC 22365 0.0 20.283253 10-11 >>END_MODULE