FastQCFastQC Report
Thu 26 May 2016
SRR938786_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938786_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764304
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167490.6059029687038763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123880.44814173839056776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85100.3078532607122806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28130.10176160075013457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83300.049.5842931
GTATCAA147600.040.6711431
ATCAACG191050.031.3390033
TATCAAC192000.031.0850982
TCAACGC193850.030.8863374
CAACGCA197300.030.322215
AACGCAG202100.029.6703116
ACGCAGA226350.026.2400827
GTACATG147150.026.1956961
CGCAGAG228850.025.7875928
TACATGG149250.025.4619122
ACATGGG149950.024.4571513
GCAGAGT238100.023.9472089
GAGTACT134400.022.24842812-13
CATGGGA91750.021.7178124
CATGGGG58150.021.7022514
AGTACTT139050.021.5214812-13
AGAGTAC217000.021.18178210-11
GTGGTAT41000.020.6350481
CAGAGTA234250.020.21964610-11
Produced by FastQC (version 0.11.5)