FastQCFastQC Report
Thu 26 May 2016
SRR938786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764304
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157260.5688954615700733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130270.4712578645474593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88570.32040614925131244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30100.10888816859506045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89000.048.5438421
GTATCAA152450.039.326151
ATCAACG193600.030.59693
TATCAAC194550.030.4718912
TCAACGC195900.030.2619024
CAACGCA198550.029.881915
AACGCAG204000.029.0952766
ACGCAGA231250.025.4406057
GTACATG149400.025.3831831
CGCAGAG233650.024.9965328
TACATGG152400.024.3551062
TAGCGCG1000.001661461123.732214
GCAGAGT243150.023.5711179
ACATGGG151950.023.4901263
GAGTACT138200.022.62458412-13
CATGGGG57050.020.8826834
AGTACTT143000.020.8200112-13
GTACTTT153950.020.35618414-15
AGAGTAC221550.020.35558510-11
CAGAGTA239400.020.11616110-11
Produced by FastQC (version 0.11.5)